pycom02g02630

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012884
Physical Location & Seq
Forward (+)
1650511 .. 1651465
955 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g02630.4

Sequence Viewer

Length: 714 bp
ATGAACATTTTGGACAAAATATTCAGATTATCAACCTCGGATCTCTTTTTGATAATCCACCAAATCCGAAGGTTCAGACAATTAAAAATTAATTCATTGATTAAAGTTATTATAAATTTTAAAGTGAATCCGGATCCTGATTCTCCTAAAAAAAGTACCGGGGGACATGAAGGGAACAAAAAATGTGACCATAAACGCACCTGGTTTCTCATCATTTTCTGGAATCGAAAGAAGAGAAATTTACAGGAAAACATGATCAAACCAGAACAAGTTGTGTTTTCTCTTGTTTTTGCCCTGTTAGGGTTAGGGTTCTTGCGTTTGCATGCTGCTTCTAGTTCTCTCACTCCGGCCTTGCAGGCCGAGAGGAGCTGCCCTTATACAGTGATCATAAAAACCAGCTGCTCTTCCACCATCTCCACCAGAGACAGGATCAGCCTTGCTTACGGTGATGGTCACGGCAATGAGGTGTATGCAAAGAGGCTTGATGATCCAGTTTCAAGGACGTTTGAGAGGTGCTCCTCCGATAAGTTCCACATAGACGGGCCATGTGCGCGCGACATCTGTTATCTCAACCTTCTGAGGAATGGATCCGACGGTTGGAAACCTGAGTTCGTCAAGATCTCGGGTCCACATACAAACCCTATTATCTTTGACTTCAACACCTTCCTGCCCAACCGAGTGTGGTTTGGACACAATCTGTGTCATCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

238

Amino Acids

27.18

Weight (kDa)

9.63

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41475
fragaria_vesca FvH4_6g29960 FvH4_6g29960
malus_domestica MD00G1023000.v1.1 MD02G1031500.v1.1
prunus_persica Prupe.3G084200_v2.0.a1 Prupe.7G243200_v2.0.a1
pyrus_communis pycom02g02630 pycom17g21450
rosa_chinensis RchiOBHm_Chr2g0135961
rosa_laevigata RLG00000019492
rosa_multiflora Rmu_ssc0000343.1_g000020
rosa_roxburghii Rroxscaffold_2G00108740
rosa_samantha Rh2AG378900 Rh2BG385400 Rh2CG364500 Rh2DG401700
rosa_wichuraiana Rw2G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AccII CGCG 2 cut(s) 553, 555
AccIII TCCGGA 1 cut(s) 130
AclWI GGATC 7 cut(s) 48, 128, 141, 437, 482, 582, 595
AcsI RAATTY 2 cut(s) 115, 238
AfaI GTAC 1 cut(s) 157
AfiI CCNNNNNNNGG 3 cut(s) 300, 426, 597
AgsI TTSAA 2 cut(s) 498, 658
AjnI CCWGG 1 cut(s) 200
AluBI AGCT 2 cut(s) 369, 399
AluI AGCT 2 cut(s) 369, 399
Alw21I GWGCWC 1 cut(s) 518
Alw26I GTCTC 1 cut(s) 417
AlwI GGATC 7 cut(s) 48, 128, 141, 437, 482, 582, 595
Ama87I CYCGRG 1 cut(s) 622
Aor13HI TCCGGA 1 cut(s) 130
AoxI GGCC 3 cut(s) 348, 357, 542
ApeKI GCWGC 3 cut(s) 326, 369, 399
ApoI RAATTY 2 cut(s) 115, 238
AseI ATTAAT 1 cut(s) 90
AspLEI GCGC 2 cut(s) 553, 555
AspS9I GGNCC 2 cut(s) 542, 626
AsuC2I CCSGG 1 cut(s) 160
AsuHPI GGTGA 1 cut(s) 458
AvaI CYCGRG 1 cut(s) 622
AvaII GGWCC 1 cut(s) 626
BamHI GGATCC 2 cut(s) 133, 587
Bbv12I GWGCWC 1 cut(s) 518
BbvI GCAGC 3 cut(s) 313, 356, 386
BccI CCATC 2 cut(s) 419, 443
BceAI ACGGC 1 cut(s) 472
BciT130I CCWGG 1 cut(s) 202
BclI TGATCA 2 cut(s) 255, 384
BcnI CCSGG 1 cut(s) 160
BcoDI GTCTC 1 cut(s) 417
BfaI CTAG 1 cut(s) 333
BglI GCCNNNNNGGC 1 cut(s) 356
BglII AGATCT 1 cut(s) 618
BisI GCNGC 3 cut(s) 327, 370, 400
BlsI GCNGC 3 cut(s) 328, 371, 401
Bme1390I CCNGG 2 cut(s) 160, 202
Bme18I GGWCC 1 cut(s) 626
BmeT110I CYCGRG 1 cut(s) 622
BmgT120I GGNCC 2 cut(s) 542, 626
BmiI GGNNCC 3 cut(s) 135, 589, 627
BmrFI CCNGG 2 cut(s) 160, 202
BplI GAGNNNNNCTC 2 cut(s) 500, 532
BpuMI CCSGG 1 cut(s) 160
BsaJI CCNNGG 2 cut(s) 36, 159
BsaWI WCCGGW 1 cut(s) 130
Bsc4I CCNNNNNNNGG 3 cut(s) 300, 426, 597
Bse1I ACTGG 1 cut(s) 491
Bse3DI GCAATG 1 cut(s) 466
BseAI TCCGGA 1 cut(s) 130
BseBI CCWGG 1 cut(s) 202
BseDI CCNNGG 2 cut(s) 36, 159
BseLI CCNNNNNNNGG 3 cut(s) 300, 426, 597
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 2 cut(s) 569, 597
BseNI ACTGG 1 cut(s) 491
BsePI GCGCGC 1 cut(s) 551
BseRI GAGGAG 2 cut(s) 379, 508
BseXI GCAGC 3 cut(s) 313, 356, 386
Bsh1236I CGCG 2 cut(s) 553, 555
BshFI GGCC 3 cut(s) 350, 359, 544
BsiHKAI GWGCWC 1 cut(s) 518
BsiHKCI CYCGRG 1 cut(s) 622
BsiSI CCGG 3 cut(s) 131, 159, 347
BslFI GGGAC 1 cut(s) 177
BslI CCNNNNNNNGG 3 cut(s) 300, 426, 597
BsmAI GTCTC 1 cut(s) 417
BsmFI GGGAC 1 cut(s) 177
BsnI GGCC 3 cut(s) 350, 359, 544
BsoBI CYCGRG 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 518
Bsp13I TCCGGA 1 cut(s) 130
Bsp143I GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
BspANI GGCC 3 cut(s) 350, 359, 544
BspCNI CTCAG 2 cut(s) 570, 598
BspEI TCCGGA 1 cut(s) 130
BspFNI CGCG 2 cut(s) 553, 555
BspLI GGNNCC 3 cut(s) 135, 589, 627
BspPI GGATC 7 cut(s) 48, 128, 141, 437, 482, 582, 595
BspQI GCTCTTC 1 cut(s) 409
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 1 cut(s) 491
BssECI CCNNGG 2 cut(s) 36, 159
BssHII GCGCGC 1 cut(s) 551
BssMI GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
Bst2UI CCWGG 1 cut(s) 202
Bst4CI ACNGT 3 cut(s) 382, 446, 596
Bst6I CTCTTC 2 cut(s) 227, 409
BstC8I GCNNGC 3 cut(s) 324, 357, 553
BstDEI CTNAG 2 cut(s) 578, 606
BstFNI CGCG 2 cut(s) 553, 555
BstHHI GCGC 2 cut(s) 553, 555
BstKTI GATC 8 cut(s) 43, 136, 258, 387, 432, 490, 590, 621
BstMAI GTCTC 1 cut(s) 417
BstMBI GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
BstMWI GCNNNNNNNGC 2 cut(s) 356, 550
BstNI CCWGG 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 158, 200
BstUI CGCG 2 cut(s) 553, 555
BstV1I GCAGC 3 cut(s) 313, 356, 386
BstX2I RGATCY 4 cut(s) 40, 133, 587, 618
BstYI RGATCY 4 cut(s) 40, 133, 587, 618
BsuRI GGCC 3 cut(s) 350, 359, 544
BtsIMutI CAGTG 1 cut(s) 387
Cac8I GCNNGC 3 cut(s) 324, 357, 553
CfoI GCGC 2 cut(s) 553, 555
Cfr13I GGNCC 2 cut(s) 542, 626
CsiI ACCWGGT 1 cut(s) 200
Csp6I GTAC 1 cut(s) 156
CviAII CATG 5 cut(s) 167, 253, 323, 546, 707
CviJI RGCY 7 cut(s) 350, 359, 369, 399, 435, 481, 544
CviKI_1 RGCY 7 cut(s) 350, 359, 369, 399, 435, 481, 544
CviQI GTAC 1 cut(s) 156
DdeI CTNAG 2 cut(s) 578, 606
DpnI GATC 8 cut(s) 42, 135, 257, 386, 431, 489, 589, 620
DpnII GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
DraI TTTAAA 1 cut(s) 121
Eam1104I CTCTTC 2 cut(s) 227, 409
EarI CTCTTC 2 cut(s) 227, 409
Eco47I GGWCC 1 cut(s) 626
Eco88I CYCGRG 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 200
FaeI CATG 5 cut(s) 170, 256, 326, 549, 710
FaqI GGGAC 1 cut(s) 177
FatI CATG 5 cut(s) 166, 252, 322, 545, 706
FbaI TGATCA 2 cut(s) 255, 384
Fnu4HI GCNGC 3 cut(s) 327, 370, 400
Fsp4HI GCNGC 3 cut(s) 327, 370, 400
FspBI CTAG 1 cut(s) 333
GlaI GCGC 2 cut(s) 552, 554
GluI GCNGC 3 cut(s) 327, 370, 400
HaeIII GGCC 3 cut(s) 350, 359, 544
HapII CCGG 3 cut(s) 131, 159, 347
HhaI GCGC 2 cut(s) 553, 555
Hin1II CATG 5 cut(s) 170, 256, 326, 549, 710
Hin6I GCGC 2 cut(s) 551, 553
HinP1I GCGC 2 cut(s) 551, 553
HinfI GANTC 3 cut(s) 127, 140, 223
HpaII CCGG 3 cut(s) 131, 159, 347
HphI GGTGA 1 cut(s) 458
Hpy166II GTNNAC 1 cut(s) 629
Hpy188I TCNGA 7 cut(s) 26, 40, 68, 77, 523, 579, 592
Hpy188III TCNNGA 4 cut(s) 131, 137, 220, 616
Hpy8I GTNNAC 1 cut(s) 629
Hpy99I CGWCG 1 cut(s) 596
HpyAV CCTTC 4 cut(s) 63, 164, 584, 673
HpyCH4III ACNGT 3 cut(s) 382, 446, 596
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 3 cut(s) 322, 355, 473
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 550
HpyF3I CTNAG 2 cut(s) 578, 606
HpySE526I ACGT 1 cut(s) 503
Hsp92II CATG 5 cut(s) 170, 256, 326, 549, 710
HspAI GCGC 2 cut(s) 551, 553
Kpn2I TCCGGA 1 cut(s) 130
Ksp22I TGATCA 2 cut(s) 255, 384
Kzo9I GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 2 cut(s) 366, 521
Lsp1109I GCAGC 3 cut(s) 313, 356, 386
MabI ACCWGGT 1 cut(s) 200
MaeI CTAG 1 cut(s) 333
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 2 cut(s) 185, 452
MalI GATC 8 cut(s) 42, 135, 257, 386, 431, 489, 589, 620
MboI GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
MboII GAAGA 2 cut(s) 244, 396
MflI RGATCY 4 cut(s) 40, 133, 587, 618
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 5 cut(s) 80, 87, 91, 115, 238
MmeI TCCRAC 2 cut(s) 578, 615
MnlI CCTC 7 cut(s) 46, 357, 457, 471, 504, 529, 573
MroI TCCGGA 1 cut(s) 130
MseI TTAA 4 cut(s) 83, 90, 102, 120
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 399
MspI CCGG 3 cut(s) 131, 159, 347
MspR9I CCNGG 2 cut(s) 160, 202
MvaI CCWGG 1 cut(s) 202
MvnI CGCG 2 cut(s) 553, 555
MwoI GCNNNNNNNGC 2 cut(s) 356, 550
NciI CCSGG 1 cut(s) 160
NdeII GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
NlaIII CATG 5 cut(s) 170, 256, 326, 549, 710
NlaIV GGNNCC 3 cut(s) 135, 589, 627
NmeAIII GCCGAG 1 cut(s) 385
NmuCI GTSAC 2 cut(s) 185, 452
NspI RCATGY 1 cut(s) 326
PaeI GCATGC 1 cut(s) 326
PauI GCGCGC 1 cut(s) 551
PciSI GCTCTTC 1 cut(s) 409
PfeI GAWTC 3 cut(s) 127, 140, 223
PkrI GCNGC 3 cut(s) 328, 371, 401
PshBI ATTAAT 1 cut(s) 90
PsiI TTATAA 1 cut(s) 113
Psp6I CCWGG 1 cut(s) 200
PspGI CCWGG 1 cut(s) 200
PspN4I GGNNCC 3 cut(s) 135, 589, 627
PspPI GGNCC 2 cut(s) 542, 626
PsuI RGATCY 4 cut(s) 40, 133, 587, 618
PteI GCGCGC 1 cut(s) 551
PvuII CAGCTG 1 cut(s) 399
RsaI GTAC 1 cut(s) 157
RsaNI GTAC 1 cut(s) 156
RseI CAYNNNNRTG 1 cut(s) 459
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 4 cut(s) 83, 90, 102, 120
SatI GCNGC 3 cut(s) 327, 370, 400
Sau3AI GATC 8 cut(s) 40, 133, 255, 384, 429, 487, 587, 618
Sau96I GGNCC 2 cut(s) 542, 626
ScrFI CCNGG 2 cut(s) 160, 202
SduI GDGCHC 1 cut(s) 518
SexAI ACCWGGT 1 cut(s) 200
SfiI GGCCNNNNNGGCC 1 cut(s) 356
SinI GGWCC 1 cut(s) 626
SmiMI CAYNNNNRTG 1 cut(s) 459
SphI GCATGC 1 cut(s) 326
Sse9I AATT 5 cut(s) 80, 87, 91, 115, 238
SspI AATATT 1 cut(s) 21
SspMI CTAG 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 158, 200
TaaI ACNGT 3 cut(s) 382, 446, 596
TaiI ACGT 1 cut(s) 506
TaqI TCGA 1 cut(s) 226
TasI AATT 5 cut(s) 80, 87, 91, 115, 238
TfiI GAWTC 3 cut(s) 127, 140, 223
Tru1I TTAA 4 cut(s) 83, 90, 102, 120
Tru9I TTAA 4 cut(s) 83, 90, 102, 120
TscAI CASTG 1 cut(s) 387
TseFI GTSAC 2 cut(s) 185, 452
TseI GCWGC 3 cut(s) 326, 369, 399
Tsp45I GTSAC 2 cut(s) 185, 452
TspDTI ATGAA 3 cut(s) 17, 84, 183
TspRI CASTG 1 cut(s) 387
VpaK11BI GGWCC 1 cut(s) 626
VspI ATTAAT 1 cut(s) 90
XapI RAATTY 2 cut(s) 115, 238
XceI RCATGY 1 cut(s) 326
XspI CTAG 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.