MD02G1031500.v1.1

Embryo-specific protein 3, (ATS3)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012884
Physical Location & Seq
Forward (+)
2461897 .. 2462730
834 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1031500.v1.1.491

Sequence Viewer

Length: 462 bp
ATGATCAAACCAGAACAAGTTGTGTTTTCTCTTGTTTTTGCCCTTTTAGGGTTAGGGTTCTTGCGTTTGCATGCTGCTTCTAGTTCTCTCACTCCGGCTCTGCAGGCCAAGAGGAGCTGCCCTTACACAGTGATCATAAAAACCAGCTGCTCTTCCACCACCTCCACTAGAGACAGGATCAGCCTTGCTTATGGTGATGCTCACGGCAATGAGGTGTATGCAAAGAGGCTTGATGATCCAGTTTCAAGGACGTTTGAGATGTGCTCCTCCGATAAGTTCCACATAGACGGGCCATGTGCGCGCGACATCTGTTATCTCAACCTTCTGAGGAATGGATCCGACGGTTGGAAACCTGAGTTCGTCAAGATCTCGGGTCCACATACAAACCCTATTATCTTTGACTTCAACACCTTCCTGCCCAACCGAGTGTGGTTTGGACACAATCTGTGTCGTCATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ATS3

Protein Analysis

154

Amino Acids

17.09

Weight (kDa)

8.81

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATS3 PF06232 34 - 150 1.5e-49 Embryo-specific protein 3, (ATS3)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012884)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G41475
fragaria_vesca FvH4_6g29960 FvH4_6g29960
malus_domestica MD00G1023000.v1.1 MD02G1031500.v1.1
prunus_persica Prupe.3G084200_v2.0.a1 Prupe.7G243200_v2.0.a1
pyrus_communis pycom02g02630 pycom17g21450
rosa_chinensis RchiOBHm_Chr2g0135961
rosa_laevigata RLG00000019492
rosa_multiflora Rmu_ssc0000343.1_g000020
rosa_roxburghii Rroxscaffold_2G00108740
rosa_samantha Rh2AG378900 Rh2BG385400 Rh2CG364500 Rh2DG401700
rosa_wichuraiana Rw2G030870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 301, 303
AclWI GGATC 4 cut(s) 185, 230, 330, 343
AfiI CCNNNNNNNGG 2 cut(s) 48, 345
AgsI TTSAA 2 cut(s) 246, 406
AluBI AGCT 2 cut(s) 117, 147
AluI AGCT 2 cut(s) 117, 147
Alw21I GWGCWC 1 cut(s) 266
Alw26I GTCTC 1 cut(s) 165
AlwI GGATC 4 cut(s) 185, 230, 330, 343
Ama87I CYCGRG 1 cut(s) 370
AoxI GGCC 2 cut(s) 105, 290
ApeKI GCWGC 3 cut(s) 74, 117, 147
AspLEI GCGC 2 cut(s) 301, 303
AspS9I GGNCC 2 cut(s) 290, 374
AsuHPI GGTGA 1 cut(s) 206
AvaI CYCGRG 1 cut(s) 370
AvaII GGWCC 1 cut(s) 374
BamHI GGATCC 1 cut(s) 335
Bbv12I GWGCWC 1 cut(s) 266
BbvI GCAGC 3 cut(s) 61, 104, 134
BceAI ACGGC 1 cut(s) 220
BclI TGATCA 2 cut(s) 3, 132
BcoDI GTCTC 1 cut(s) 165
BfaI CTAG 2 cut(s) 81, 168
BfmI CTRYAG 1 cut(s) 101
BglII AGATCT 1 cut(s) 366
BisI GCNGC 3 cut(s) 75, 118, 148
BlsI GCNGC 3 cut(s) 76, 119, 149
Bme18I GGWCC 1 cut(s) 374
BmeT110I CYCGRG 1 cut(s) 370
BmgT120I GGNCC 2 cut(s) 290, 374
BmiI GGNNCC 2 cut(s) 337, 375
BmsI GCATC 1 cut(s) 187
BplI GAGNNNNNCTC 2 cut(s) 248, 280
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 345
Bse1I ACTGG 1 cut(s) 239
Bse3DI GCAATG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 48, 345
BseMI GCAATG 1 cut(s) 214
BseMII CTCAG 2 cut(s) 317, 345
BseNI ACTGG 1 cut(s) 239
BsePI GCGCGC 1 cut(s) 299
BseRI GAGGAG 2 cut(s) 127, 256
BseXI GCAGC 3 cut(s) 61, 104, 134
Bsh1236I CGCG 2 cut(s) 301, 303
BshFI GGCC 2 cut(s) 107, 292
BsiHKAI GWGCWC 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 370
BsiSI CCGG 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 48, 345
BsmAI GTCTC 1 cut(s) 165
BsnI GGCC 2 cut(s) 107, 292
BsoBI CYCGRG 1 cut(s) 370
Bsp1286I GDGCHC 1 cut(s) 266
Bsp143I GATC 6 cut(s) 3, 132, 177, 235, 335, 366
BspANI GGCC 2 cut(s) 107, 292
BspCNI CTCAG 2 cut(s) 318, 346
BspFNI CGCG 2 cut(s) 301, 303
BspLI GGNNCC 2 cut(s) 337, 375
BspMAI CTGCAG 1 cut(s) 105
BspPI GGATC 4 cut(s) 185, 230, 330, 343
BspQI GCTCTTC 1 cut(s) 157
BsrDI GCAATG 1 cut(s) 214
BsrI ACTGG 1 cut(s) 239
BssHII GCGCGC 1 cut(s) 299
BssMI GATC 6 cut(s) 3, 132, 177, 235, 335, 366
Bst4CI ACNGT 2 cut(s) 130, 344
Bst6I CTCTTC 1 cut(s) 157
BstC8I GCNNGC 3 cut(s) 72, 105, 301
BstDEI CTNAG 2 cut(s) 326, 354
BstFNI CGCG 2 cut(s) 301, 303
BstHHI GCGC 2 cut(s) 301, 303
BstKTI GATC 6 cut(s) 6, 135, 180, 238, 338, 369
BstMAI GTCTC 1 cut(s) 165
BstMBI GATC 6 cut(s) 3, 132, 177, 235, 335, 366
BstMWI GCNNNNNNNGC 2 cut(s) 104, 298
BstNSI RCATGY 1 cut(s) 74
BstSFI CTRYAG 1 cut(s) 101
BstUI CGCG 2 cut(s) 301, 303
BstV1I GCAGC 3 cut(s) 61, 104, 134
BstX2I RGATCY 2 cut(s) 335, 366
BstYI RGATCY 2 cut(s) 335, 366
BsuRI GGCC 2 cut(s) 107, 292
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 3 cut(s) 72, 105, 301
CfoI GCGC 2 cut(s) 301, 303
Cfr13I GGNCC 2 cut(s) 290, 374
CviAII CATG 3 cut(s) 71, 294, 455
CviJI RGCY 7 cut(s) 98, 107, 117, 147, 183, 229, 292
CviKI_1 RGCY 7 cut(s) 98, 107, 117, 147, 183, 229, 292
DdeI CTNAG 2 cut(s) 326, 354
DpnI GATC 6 cut(s) 5, 134, 179, 237, 337, 368
DpnII GATC 6 cut(s) 3, 132, 177, 235, 335, 366
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 374
Eco88I CYCGRG 1 cut(s) 370
FaeI CATG 3 cut(s) 74, 297, 458
FaiI YATR 8 cut(s) 72, 137, 192, 219, 284, 295, 381, 456
FatI CATG 3 cut(s) 70, 293, 454
FbaI TGATCA 2 cut(s) 3, 132
Fnu4HI GCNGC 3 cut(s) 75, 118, 148
Fsp4HI GCNGC 3 cut(s) 75, 118, 148
FspBI CTAG 2 cut(s) 81, 168
GlaI GCGC 2 cut(s) 300, 302
GluI GCNGC 3 cut(s) 75, 118, 148
HaeIII GGCC 2 cut(s) 107, 292
HapII CCGG 1 cut(s) 95
HhaI GCGC 2 cut(s) 301, 303
Hin1II CATG 3 cut(s) 74, 297, 458
Hin6I GCGC 2 cut(s) 299, 301
HinP1I GCGC 2 cut(s) 299, 301
HpaII CCGG 1 cut(s) 95
HphI GGTGA 1 cut(s) 206
Hpy166II GTNNAC 1 cut(s) 377
Hpy188I TCNGA 3 cut(s) 271, 327, 340
Hpy188III TCNNGA 1 cut(s) 364
Hpy8I GTNNAC 1 cut(s) 377
Hpy99I CGWCG 1 cut(s) 344
HpyAV CCTTC 2 cut(s) 332, 421
HpyCH4III ACNGT 2 cut(s) 130, 344
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 3 cut(s) 70, 103, 221
HpyF10VI GCNNNNNNNGC 2 cut(s) 104, 298
HpyF3I CTNAG 2 cut(s) 326, 354
HpySE526I ACGT 1 cut(s) 251
Hsp92II CATG 3 cut(s) 74, 297, 458
HspAI GCGC 2 cut(s) 299, 301
Ksp22I TGATCA 2 cut(s) 3, 132
Kzo9I GATC 6 cut(s) 3, 132, 177, 235, 335, 366
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 2 cut(s) 114, 269
LpnPI CCDG 8 cut(s) 24, 89, 108, 157, 160, 252, 366, 428
Lsp1109I GCAGC 3 cut(s) 61, 104, 134
LweI GCATC 1 cut(s) 187
MaeI CTAG 2 cut(s) 81, 168
MaeII ACGT 1 cut(s) 251
MalI GATC 6 cut(s) 5, 134, 179, 237, 337, 368
MboI GATC 6 cut(s) 3, 132, 177, 235, 335, 366
MboII GAAGA 1 cut(s) 144
MflI RGATCY 2 cut(s) 335, 366
MhlI GDGCHC 1 cut(s) 266
MmeI TCCRAC 2 cut(s) 326, 363
MnlI CCTC 6 cut(s) 105, 172, 205, 219, 277, 321
MslI CAYNNNNRTG 1 cut(s) 207
MspA1I CMGCKG 1 cut(s) 147
MspI CCGG 1 cut(s) 95
MvnI CGCG 2 cut(s) 301, 303
MwoI GCNNNNNNNGC 2 cut(s) 104, 298
NdeII GATC 6 cut(s) 3, 132, 177, 235, 335, 366
NlaIII CATG 3 cut(s) 74, 297, 458
NlaIV GGNNCC 2 cut(s) 337, 375
NspI RCATGY 1 cut(s) 74
PaeI GCATGC 1 cut(s) 74
PauI GCGCGC 1 cut(s) 299
PciSI GCTCTTC 1 cut(s) 157
PkrI GCNGC 3 cut(s) 76, 119, 149
PspN4I GGNNCC 2 cut(s) 337, 375
PspPI GGNCC 2 cut(s) 290, 374
PstI CTGCAG 1 cut(s) 105
PsuI RGATCY 2 cut(s) 335, 366
PteI GCGCGC 1 cut(s) 299
PvuII CAGCTG 1 cut(s) 147
RseI CAYNNNNRTG 1 cut(s) 207
SapI GCTCTTC 1 cut(s) 157
SatI GCNGC 3 cut(s) 75, 118, 148
Sau3AI GATC 6 cut(s) 3, 132, 177, 235, 335, 366
Sau96I GGNCC 2 cut(s) 290, 374
SduI GDGCHC 1 cut(s) 266
SetI ASST 8 cut(s) 119, 149, 164, 216, 254, 324, 355, 413
SfaNI GCATC 1 cut(s) 187
SfcI CTRYAG 1 cut(s) 101
SinI GGWCC 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 207
SphI GCATGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 81, 168
TaaI ACNGT 2 cut(s) 130, 344
TaiI ACGT 1 cut(s) 254
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 74, 117, 147
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 374
XceI RCATGY 1 cut(s) 74
XspI CTAG 2 cut(s) 81, 168
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.