MD00G1048800.v1.1

L-galactono-1,4-lactone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0013114
Physical Location & Seq
Reverse (-)
9125401 .. 9127461
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1048800.v1.1.491

Sequence Viewer

Length: 1113 bp
ATGCTCAGAGCTCTCACTCTCAGACGCTCCGTCCAGTCCCACCACCACCACTACAACCTGAGAGCCGTCGCTTCCACCACCTTCCCTCAAAACCCACCGAACCCAGTAAGCTCAACACGCGCATTATCCACCCTTTCATCGCCGTCATCGCCGGCACCATCTTCCTCAGCCTCCGAGCTCCGCAAGTACTTGTGCTACACCGCCCTCGTCCTGTTCAGCGCCGCCGCCACCTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCATAAAAAGGCCCAGATTTTCCGGTATGCTCCGCTGCCGGAGGACCTCCACACCGTCTCCAACTGGAGCGGGACCCACGAGGTCCAGACCCGGGTTTTCCACCAGCCCGAAACGCTTGAGGAGCTGGAGAAGGTCGTGAAGGATGCCCACGAGAAGAAAACCGGGATTCGGCCAGTTGGGTCCGGATTGTCGCCGAATGGGATTGGACTGTCCAGGGCAGGGATGGTCAATTTGGCGCTGATGGACAAGGTTTTGGAGGTGGATAAGGAGAAGAAGAGGGTGAGAGTGCAGGCTGGGATAAGAGTGCAGCAGTTGGTGGATGGGATTAAGGAACATGGAATTACTCTGCAGAACTTGCCTCCATTAGGGAGCAGTAGATTGGTGGCATTTTGCAGGTGGGTTGGTGCACATGGTACTGGTTCAAGATTGCCTCCAATCGATGAGCAGGTGATCAGCATGAAAATGGTCACTCCTGCTAAGGGAACACTAGAAGTTTCTAAAGAGAAAGATCCAGAGCTGTTTTATTTAGCTCGCTGTGGCCTTGGTGGCCTTGGAGTGGTTGCTGAAGTCACCATCCAATGCGTTGATAGACAGGAGCTTGGGGAGCACACTACTGTTTCAACCTTGGCAGAGATCAAGAAAAATCAATTCATTGATAAGAAATTGCTATCCGAGAATAAACATGTGAAGTACCTTTATATTCCATATACCAACACTGTTGTTGTTGTGAAATGTAACCCTGTATCAAAATGGAAAGGTCCCCCGAAGTTCAAACCCATATATAGCAGTGATGAAGCCATACAGCACGTCCGAAATCTCTACAGGGAGTGCCTCCAGAAGTACAGGTAA

Protein Analysis

371

Amino Acids

41.28

Weight (kDa)

9.56

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_4 PF01565 120 - 251 2.1e-21 FAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47930 AT3G47930
fragaria_vesca FvH4_6g25110
malus_domestica MD00G1048800.v1.1 MD00G1074000.v1.1
prunus_persica Prupe.7G046300_v2.0.a1 Prupe.7G046300_v2.0.a1
pyrus_communis pycom14g07300
rosa_chinensis RchiOBHm_Chr2g0124941
rosa_laevigata RLG00000018835
rosa_multiflora Rmu_sc0008068.1_g000007
rosa_roxburghii Rroxscaffold_2G00118570 Rroxscaffold_2G00118860
rosa_rugosa Rorug02G0253600
rosa_samantha Rh2AG313000 Rh2CG302400 Rh2DG338500
rosa_wichuraiana Rw2G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 648, 700
Acc36I ACCTGC 2 cut(s) 648, 700
AccB1I GGYRCC 1 cut(s) 154
AccBSI CCGCTC 1 cut(s) 333
AccII CGCG 1 cut(s) 120
AccIII TCCGGA 1 cut(s) 446
AciI CCGC 6 cut(s) 181, 201, 222, 225, 296, 333
AclWI GGATC 1 cut(s) 767
AcoI YGGCCR 1 cut(s) 434
AcuI CTGAAG 1 cut(s) 849
AfaI GTAC 4 cut(s) 188, 679, 956, 1106
AfiI CCNNNNNNNGG 6 cut(s) 355, 432, 483, 629, 743, 820
AflIII ACRYGT 1 cut(s) 946
AgsI TTSAA 3 cut(s) 687, 885, 1036
AhdI GACNNNNNGTC 1 cut(s) 29
AjiI CACGTC 1 cut(s) 1072
AjnI CCWGG 1 cut(s) 476
AluBI AGCT 7 cut(s) 11, 111, 178, 388, 781, 794, 862
AluI AGCT 7 cut(s) 11, 111, 178, 388, 781, 794, 862
Alw21I GWGCWC 4 cut(s) 13, 180, 673, 873
Alw26I GTCTC 1 cut(s) 325
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 1 cut(s) 767
Ama87I CYCGRG 1 cut(s) 354
Aor13HI TCCGGA 1 cut(s) 446
AoxI GGCC 4 cut(s) 273, 434, 802, 811
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 2 cut(s) 298, 571
AspLEI GCGC 3 cut(s) 122, 221, 502
AspS9I GGNCC 6 cut(s) 274, 307, 336, 346, 444, 1022
AsuC2I CCSGG 3 cut(s) 355, 356, 427
AsuHPI GGTGA 3 cut(s) 556, 724, 826
AvaI CYCGRG 1 cut(s) 354
AvaII GGWCC 5 cut(s) 307, 336, 346, 444, 1022
BaeGI GKGCMC 1 cut(s) 673
BanI GGYRCC 1 cut(s) 154
BanII GRGCYC 2 cut(s) 13, 180
BauI CACGAG 2 cut(s) 341, 413
Bbv12I GWGCWC 4 cut(s) 13, 180, 673, 873
BbvCI CCTCAGC 1 cut(s) 166
BbvI GCAGC 2 cut(s) 285, 583
BccI CCATC 5 cut(s) 166, 481, 499, 578, 845
BceAI ACGGC 2 cut(s) 50, 127
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 1 cut(s) 714
BcnI CCSGG 3 cut(s) 355, 356, 427
BcoDI GTCTC 1 cut(s) 325
BfaI CTAG 1 cut(s) 752
BfmI CTRYAG 2 cut(s) 611, 1084
BfoI RGCGCY 2 cut(s) 222, 503
BfuAI ACCTGC 2 cut(s) 648, 700
BglI GCCNNNNNGGC 1 cut(s) 810
BisI GCNGC 4 cut(s) 222, 225, 299, 572
BlsI GCNGC 4 cut(s) 223, 226, 300, 573
BmcAI AGTACT 1 cut(s) 188
Bme1390I CCNGG 4 cut(s) 355, 356, 427, 478
Bme18I GGWCC 5 cut(s) 307, 336, 346, 444, 1022
BmeRI GACNNNNNGTC 1 cut(s) 29
BmeT110I CYCGRG 1 cut(s) 354
BmgBI CACGTC 1 cut(s) 1072
BmgT120I GGNCC 6 cut(s) 274, 307, 336, 346, 444, 1022
BmiI GGNNCC 5 cut(s) 156, 337, 338, 445, 1024
BmrFI CCNGG 4 cut(s) 355, 356, 427, 478
BmrI ACTGGG 1 cut(s) 98
BmsI GCATC 1 cut(s) 397
BmuI ACTGGG 1 cut(s) 98
BpmI CTGGAG 3 cut(s) 349, 410, 1082
Bpu10I CCTNAGC 2 cut(s) 166, 741
BpuEI CTTGAG 1 cut(s) 401
BpuMI CCSGG 3 cut(s) 355, 356, 427
Bsa29I ATCGAT 1 cut(s) 702
BsaJI CCNNGG 5 cut(s) 354, 477, 805, 814, 888
BsaWI WCCGGW 2 cut(s) 285, 446
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 6 cut(s) 355, 432, 483, 629, 743, 820
Bse118I RCCGGY 1 cut(s) 151
Bse1I ACTGG 5 cut(s) 34, 104, 332, 437, 685
BseAI TCCGGA 1 cut(s) 446
BseBI CCWGG 1 cut(s) 478
BseCI ATCGAT 1 cut(s) 702
BseDI CCNNGG 5 cut(s) 354, 477, 805, 814, 888
BseGI GGATG 4 cut(s) 412, 492, 589, 837
BseLI CCNNNNNNNGG 6 cut(s) 355, 432, 483, 629, 743, 820
BseMII CTCAG 4 cut(s) 19, 34, 50, 180
BseNI ACTGG 5 cut(s) 34, 104, 332, 437, 685
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 2 cut(s) 285, 583
BseYI CCCAGC 1 cut(s) 557
BsgI GTGCAG 2 cut(s) 572, 590
Bsh1236I CGCG 1 cut(s) 120
BshFI GGCC 4 cut(s) 275, 436, 804, 813
BshNI GGYRCC 1 cut(s) 154
BshVI ATCGAT 1 cut(s) 702
BsiHKAI GWGCWC 4 cut(s) 13, 180, 673, 873
BsiHKCI CYCGRG 1 cut(s) 354
BsiSI CCGG 6 cut(s) 152, 286, 302, 355, 426, 447
BslFI GGGAC 3 cut(s) 22, 349, 1008
BslI CCNNNNNNNGG 6 cut(s) 355, 432, 483, 629, 743, 820
BsmAI GTCTC 1 cut(s) 325
BsmBI CGTCTC 1 cut(s) 325
BsmFI GGGAC 3 cut(s) 22, 349, 1008
BsnI GGCC 4 cut(s) 275, 436, 804, 813
BsoBI CYCGRG 1 cut(s) 354
Bsp1286I GDGCHC 4 cut(s) 13, 180, 673, 873
Bsp13I TCCGGA 1 cut(s) 446
Bsp143I GATC 3 cut(s) 714, 772, 897
BspACI CCGC 6 cut(s) 181, 201, 222, 225, 296, 333
BspANI GGCC 4 cut(s) 275, 436, 804, 813
BspCNI CTCAG 4 cut(s) 18, 33, 51, 179
BspDI ATCGAT 1 cut(s) 702
BspEI TCCGGA 1 cut(s) 446
BspFNI CGCG 1 cut(s) 120
BspLI GGNNCC 5 cut(s) 156, 337, 338, 445, 1024
BspMAI CTGCAG 1 cut(s) 615
BspMI ACCTGC 2 cut(s) 648, 700
BspPI GGATC 1 cut(s) 767
BspT107I GGYRCC 1 cut(s) 154
BsrBI CCGCTC 1 cut(s) 333
BsrFI RCCGGY 1 cut(s) 151
BsrI ACTGG 5 cut(s) 34, 104, 332, 437, 685
BssAI RCCGGY 1 cut(s) 151
BssECI CCNNGG 5 cut(s) 354, 477, 805, 814, 888
BssMI GATC 3 cut(s) 714, 772, 897
BssSI CACGAG 2 cut(s) 341, 413
BssT1I CCWWGG 3 cut(s) 805, 814, 888
Bst2BI CACGAG 2 cut(s) 341, 413
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 4 cut(s) 319, 474, 880, 982
Bst6I CTCTTC 1 cut(s) 533
BstAPI GCANNNNNTGC 1 cut(s) 619
BstC8I GCNNGC 3 cut(s) 153, 555, 796
BstDEI CTNAG 5 cut(s) 5, 20, 59, 166, 741
BstENI CCTNNNNNAGG 1 cut(s) 627
BstF5I GGATG 4 cut(s) 412, 492, 589, 837
BstFNI CGCG 1 cut(s) 120
BstH2I RGCGCY 2 cut(s) 222, 503
BstHHI GCGC 3 cut(s) 122, 221, 502
BstKTI GATC 3 cut(s) 717, 775, 900
BstMAI GTCTC 1 cut(s) 325
BstMBI GATC 3 cut(s) 714, 772, 897
BstMWI GCNNNNNNNGC 7 cut(s) 117, 148, 376, 385, 619, 810, 868
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 950
BstSCI CCNGG 4 cut(s) 353, 354, 425, 476
BstSFI CTRYAG 2 cut(s) 611, 1084
BstSLI GKGCMC 1 cut(s) 673
BstUI CGCG 1 cut(s) 120
BstV1I GCAGC 2 cut(s) 285, 583
BstX2I RGATCY 1 cut(s) 772
BstYI RGATCY 1 cut(s) 772
Bsu15I ATCGAT 1 cut(s) 702
BsuRI GGCC 4 cut(s) 275, 436, 804, 813
BsuTUI ATCGAT 1 cut(s) 702
BtgZI GCGATG 2 cut(s) 123, 132
BtrI CACGTC 1 cut(s) 1072
BtsCI GGATG 4 cut(s) 412, 492, 589, 837
BtsI GCAGTG 1 cut(s) 1057
BtsIMutI CAGTG 2 cut(s) 978, 1057
BveI ACCTGC 2 cut(s) 648, 700
Cac8I GCNNGC 3 cut(s) 153, 555, 796
CfoI GCGC 3 cut(s) 122, 221, 502
Cfr10I RCCGGY 1 cut(s) 151
Cfr13I GGNCC 6 cut(s) 274, 307, 336, 346, 444, 1022
Cfr9I CCCGGG 1 cut(s) 354
ClaI ATCGAT 1 cut(s) 702
CseI GACGC 1 cut(s) 33
Csp6I GTAC 4 cut(s) 187, 678, 955, 1105
CviAII CATG 4 cut(s) 599, 674, 721, 947
CviQI GTAC 4 cut(s) 187, 678, 955, 1105
DdeI CTNAG 5 cut(s) 5, 20, 59, 166, 741
DpnI GATC 3 cut(s) 716, 774, 899
DpnII GATC 3 cut(s) 714, 772, 897
DriI GACNNNNNGTC 1 cut(s) 29
EaeI YGGCCR 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 533
Eam1105I GACNNNNNGTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 533
Ecl136II GAGCTC 2 cut(s) 11, 178
Eco130I CCWWGG 3 cut(s) 805, 814, 888
Eco24I GRGCYC 2 cut(s) 13, 180
Eco47I GGWCC 5 cut(s) 307, 336, 346, 444, 1022
Eco53kI GAGCTC 2 cut(s) 11, 178
Eco57I CTGAAG 1 cut(s) 849
Eco88I CYCGRG 1 cut(s) 354
EcoICRI GAGCTC 2 cut(s) 11, 178
EcoNI CCTNNNNNAGG 1 cut(s) 627
EcoO109I RGGNCCY 3 cut(s) 307, 336, 1022
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 3 cut(s) 805, 814, 888
EcoT38I GRGCYC 2 cut(s) 13, 180
ErhI CCWWGG 3 cut(s) 805, 814, 888
Esp3I CGTCTC 1 cut(s) 325
FaeI CATG 4 cut(s) 602, 677, 724, 950
FaqI GGGAC 3 cut(s) 22, 349, 1008
FatI CATG 4 cut(s) 598, 673, 720, 946
FauI CCCGC 1 cut(s) 326
FbaI TGATCA 1 cut(s) 714
Fnu4HI GCNGC 4 cut(s) 222, 225, 299, 572
FokI GGATG 4 cut(s) 419, 499, 596, 824
FriOI GRGCYC 2 cut(s) 13, 180
Fsp4HI GCNGC 4 cut(s) 222, 225, 299, 572
FspBI CTAG 1 cut(s) 752
GlaI GCGC 3 cut(s) 121, 220, 501
GluI GCNGC 4 cut(s) 222, 225, 299, 572
GsaI CCCAGC 1 cut(s) 561
GsuI CTGGAG 3 cut(s) 349, 410, 1082
HaeII RGCGCY 2 cut(s) 222, 503
HaeIII GGCC 4 cut(s) 275, 436, 804, 813
HapII CCGG 6 cut(s) 152, 286, 302, 355, 426, 447
HgaI GACGC 1 cut(s) 33
HhaI GCGC 3 cut(s) 122, 221, 502
Hin1II CATG 4 cut(s) 602, 677, 724, 950
Hin6I GCGC 3 cut(s) 120, 219, 500
HinP1I GCGC 3 cut(s) 120, 219, 500
HinfI GANTC 1 cut(s) 430
HpaII CCGG 6 cut(s) 152, 286, 302, 355, 426, 447
HphI GGTGA 3 cut(s) 556, 724, 826
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 5 cut(s) 8, 23, 175, 937, 1076
Hpy188III TCNNGA 7 cut(s) 349, 400, 447, 687, 776, 901, 1099
Hpy8I GTNNAC 1 cut(s) 671
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 4 cut(s) 91, 250, 388, 397
HpyCH4III ACNGT 4 cut(s) 319, 474, 880, 982
HpyCH4IV ACGT 1 cut(s) 1071
HpyCH4V TGCA 5 cut(s) 553, 571, 613, 657, 671
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 148, 376, 385, 619, 810, 868
HpyF3I CTNAG 5 cut(s) 5, 20, 59, 166, 741
HpySE526I ACGT 1 cut(s) 1071
Hsp92II CATG 4 cut(s) 602, 677, 724, 950
HspAI GCGC 3 cut(s) 120, 219, 500
KflI GGGWCCC 1 cut(s) 336
Kpn2I TCCGGA 1 cut(s) 446
KroI GCCGGC 1 cut(s) 151
KroNI GCCGGC 1 cut(s) 153
Ksp22I TGATCA 1 cut(s) 714
Kzo9I GATC 3 cut(s) 714, 772, 897
LmnI GCTCC 8 cut(s) 32, 183, 298, 330, 385, 633, 859, 868
Lsp1109I GCAGC 2 cut(s) 285, 583
LweI GCATC 1 cut(s) 397
MaeI CTAG 1 cut(s) 752
MaeII ACGT 1 cut(s) 1071
MaeIII GTNAC 3 cut(s) 730, 832, 998
MalI GATC 3 cut(s) 716, 774, 899
MbiI CCGCTC 1 cut(s) 333
MboI GATC 3 cut(s) 714, 772, 897
MboII GAAGA 4 cut(s) 153, 430, 547, 550
MflI RGATCY 1 cut(s) 772
MhlI GDGCHC 4 cut(s) 13, 180, 673, 873
MluCI AATT 4 cut(s) 493, 603, 911, 926
MmeI TCCRAC 1 cut(s) 348
MroI TCCGGA 1 cut(s) 446
MroNI GCCGGC 1 cut(s) 151
MseI TTAA 1 cut(s) 591
MslI CAYNNNNRTG 1 cut(s) 725
MspA1I CMGCKG 1 cut(s) 298
MspI CCGG 6 cut(s) 152, 286, 302, 355, 426, 447
MspR9I CCNGG 4 cut(s) 355, 356, 427, 478
MvaI CCWGG 1 cut(s) 478
MvnI CGCG 1 cut(s) 120
MwoI GCNNNNNNNGC 7 cut(s) 117, 148, 376, 385, 619, 810, 868
NaeI GCCGGC 1 cut(s) 153
NciI CCSGG 3 cut(s) 355, 356, 427
NdeII GATC 3 cut(s) 714, 772, 897
NgoMIV GCCGGC 1 cut(s) 151
NlaIII CATG 4 cut(s) 602, 677, 724, 950
NlaIV GGNNCC 5 cut(s) 156, 337, 338, 445, 1024
NmuCI GTSAC 2 cut(s) 730, 832
NspI RCATGY 1 cut(s) 950
PaqCI CACCTGC 2 cut(s) 648, 700
PciI ACATGT 1 cut(s) 946
PdiI GCCGGC 1 cut(s) 153
PfeI GAWTC 1 cut(s) 430
PkrI GCNGC 4 cut(s) 223, 226, 300, 573
PpuMI RGGWCCY 3 cut(s) 307, 336, 1022
PscI ACATGT 1 cut(s) 946
Psp124BI GAGCTC 2 cut(s) 13, 180
Psp5II RGGWCCY 3 cut(s) 307, 336, 1022
Psp6I CCWGG 1 cut(s) 476
PspFI CCCAGC 1 cut(s) 557
PspGI CCWGG 1 cut(s) 476
PspN4I GGNNCC 5 cut(s) 156, 337, 338, 445, 1024
PspPI GGNCC 6 cut(s) 274, 307, 336, 346, 444, 1022
PspPPI RGGWCCY 3 cut(s) 307, 336, 1022
PstI CTGCAG 1 cut(s) 615
PsuI RGATCY 1 cut(s) 772
RsaI GTAC 4 cut(s) 188, 679, 956, 1106
RsaNI GTAC 4 cut(s) 187, 678, 955, 1105
RseI CAYNNNNRTG 1 cut(s) 725
SacI GAGCTC 2 cut(s) 13, 180
SaqAI TTAA 1 cut(s) 591
SatI GCNGC 4 cut(s) 222, 225, 299, 572
Sau3AI GATC 3 cut(s) 714, 772, 897
Sau96I GGNCC 6 cut(s) 274, 307, 336, 346, 444, 1022
ScaI AGTACT 1 cut(s) 188
ScrFI CCNGG 4 cut(s) 355, 356, 427, 478
SduI GDGCHC 4 cut(s) 13, 180, 673, 873
SfaNI GCATC 1 cut(s) 397
SfcI CTRYAG 2 cut(s) 611, 1084
SfiI GGCCNNNNNGGCC 1 cut(s) 810
SinI GGWCC 5 cut(s) 307, 336, 346, 444, 1022
SmaI CCCGGG 1 cut(s) 356
SmiMI CAYNNNNRTG 1 cut(s) 725
SmlI CTYRAG 1 cut(s) 380
SmoI CTYRAG 1 cut(s) 380
Sse9I AATT 4 cut(s) 493, 603, 911, 926
SsiI CCGC 6 cut(s) 181, 201, 222, 225, 296, 333
SspMI CTAG 1 cut(s) 752
SstI GAGCTC 2 cut(s) 13, 180
StyD4I CCNGG 4 cut(s) 353, 354, 425, 476
StyI CCWWGG 3 cut(s) 805, 814, 888
TaaI ACNGT 4 cut(s) 319, 474, 880, 982
TaiI ACGT 1 cut(s) 1074
TaqI TCGA 1 cut(s) 702
TasI AATT 4 cut(s) 493, 603, 911, 926
TatI WGTACW 2 cut(s) 186, 1104
TauI GCSGC 2 cut(s) 224, 227
TfiI GAWTC 1 cut(s) 430
Tru1I TTAA 1 cut(s) 591
Tru9I TTAA 1 cut(s) 591
TscAI CASTG 2 cut(s) 985, 1057
TseFI GTSAC 2 cut(s) 730, 832
TseI GCWGC 2 cut(s) 298, 571
Tsp45I GTSAC 2 cut(s) 730, 832
TspDTI ATGAA 4 cut(s) 126, 737, 904, 1071
TspGWI ACGGA 1 cut(s) 19
TspMI CCCGGG 1 cut(s) 354
TspRI CASTG 2 cut(s) 985, 1057
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 5 cut(s) 307, 336, 346, 444, 1022
XagI CCTNNNNNAGG 1 cut(s) 627
XceI RCATGY 1 cut(s) 950
XcmI CCANNNNNNNNNTGG 1 cut(s) 484
XmaI CCCGGG 1 cut(s) 354
XspI CTAG 1 cut(s) 752
ZrmI AGTACT 1 cut(s) 188
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.