Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr2

Orthogroup ID

OG0013114

Physical Location & Seq

Genomic Coordinates Reverse (-)

37762366 .. 37769122

Gene Structure 6757 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw2G025280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1785 bp

ATGCAGAGAGCTCTCACTGTGAAGCGCTCGCTCCAATCCCTCCACAGAATCGCCAATTCCACCACTAAAAACCCACTAAGCACAGGGCGTGCATTTTGCAATGCACCGTCACCGTCACCGGCACCGTCGGCGTCCTCCGCATCCGCATCAGCATCAGCATCTGAGGTCCGCAAGTACATGGGGTACACAGCCCTGGTCTTATTCTGCGGTGCCGCCACCTACTACTCCTTCCCATTTCCCGAGAACGCCAAGCACAAGAAGGCCCAAATCTTCCGGTACGCCCCTCTACCTGAGGAGCTCCACACGGTCTCAAACTGGAGCGGGACCCACGAGGTCCAGACCCGGGTTTTCCACCAGCCCGAGACCCTTGAGGAGCTGCAGAAAGTAGTCAAGGAGGCGAATGAGAACAAGTACCGGATTCGGCCGGTCGGCTCTGGGTTGTCCCCCAATGGGATTGGATTGTCCAGGGCTGGGATGGTCAATTTGGCGCTCATGGATCAGGTTTTGGAGGTGGATAAGGAGAAGAAGAGAGTGAGAGTGCAGGCTGGGATCAGGGTCCAGCAATTGGTGGATGGGATTAAGGATCAGGGCCTTACTTTGCAGAACTTTGCCTCCATAAGGGAGCAACAGATTGGTGGCATTGTGCAGGTTGGTGCACATGGTACTGGTGCGAGGTTGCCTCCGATTGATGAGCAGGTGATCAGCATGAAATTGGTCACTCCTGCTAAGGGAACAATTGAGGTCTCCAAGGAGAAAGATCCAGAGCTGTTTTATTTAGCTCGCTGTGGCCTCGGCGGCCTGGGAGTTGTTGCTGAAGTTACCATCCAATGTGTTGACAGACAGGAGCTTGTAGAGCACACTACTGTTTCAAACATGGAAGAAATCAAGAAAAATCACAAGAAATTGCTCTCTGAGAACAAACATGTGAAGTATCTCTATATTCCATATACTGATACTGTTGTGGTTGTGACGTGCAACCCTGTCTCGAAATGGAAAGGTCCCCCAAAGTTCAAACCCAAATATACAACGGATGAAGCCATTCAGCACGTTGGTGATCTCTACAGGGAATGCCTCAAGAAGTACAGAGTAGTACCAGACAACAGTGTAGACATAAATGAGCTCTCTTTTACAGAGTTGAGAGATAAACTACTGGCCCTAAATCCTCTCAACAAAGATCATATCGTGAAAGTGAATCAAGCTGAGGCAGAGTTCTGGAGGAAGTCGGAGGGATACAGAGTAGGATGGAGTGATGAAATTCTGGGCTTTGATTGTGGTGGCCAACAGTGGGTTTCGGAGACTTGTTTTCCAGCTGGAACCCTTGCCAAACCAAGTATGAAAGACCTTGAATACATAGCAGATCTAAAACAGCTAATAGAGAAAGAAGAGATACCTGCACCTGCTCCAATAGAGCAGCGCTGGACAGCCAGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCTCAAGAGAGGATGATATATTTTCATGGGTTGGCATAATCATGTACCTTCCAACAACAGATGCCCGCCAAAGAAAGGATATTACAGAAGAGTTTTTCCACTACAGGCATCTAACCCAGACACGGTTGTGGGATACGTATTCTTCTTATGAACACTGGGCCAAGATTGAGGTCCCAAAGGACAAAGAACAGCTTACCGCTCTGCAGGCAAGACTAAGGAAGCGTTTTCCAGTTGACGCCTACAACAAAGCACGAATGGAATTAGACCCAAACCGGATCCTTTCAAACATCAAGCTGGAAAAGCTTTTCCCATTGTCTGATACCATTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0008152 GO:0009536 GO:0016491 GO:0016627 GO:0016632 GO:0016633 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0080049

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 ko01110 map00053 map01100 map01110

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

594

Amino Acids

67.03

Weight (kDa)

8.8

Isoelectric Point (pI)

42.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	116 - 246	5.3e-29	FAD binding domain
ALO	PF04030	273 - 584	4.2e-19	D-arabinono-1,4-lactone oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47930 AT3G47930
fragaria_vesca	FvH4_6g25110
malus_domestica	MD00G1048800.v1.1 MD00G1074000.v1.1
prunus_persica	Prupe.7G046300_v2.0.a1 Prupe.7G046300_v2.0.a1
pyrus_communis	pycom14g07300
rosa_chinensis	RchiOBHm_Chr2g0124941
rosa_laevigata	RLG00000018835
rosa_multiflora	Rmu_sc0008068.1_g000007
rosa_roxburghii	Rroxscaffold_2G00118570 Rroxscaffold_2G00118860
rosa_rugosa	Rorug02G0253600
rosa_samantha	Rh2AG313000 Rh2CG302400 Rh2DG338500
rosa_wichuraiana	Rw2G025280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	685, 1405
Acc36I	ACCTGC	4 cut(s)	637, 685, 1399, 1405
AccB1I	GGYRCC	2 cut(s)	121, 209
AccB7I	CCANNNNNTGG	1 cut(s)	565
AccBSI	CCGCTC	2 cut(s)	321, 1655
AccI	GTMKAC	1 cut(s)	1107
AciI	CCGC	9 cut(s)	138, 144, 169, 207, 213, 321, 795, 1522, 1653
AclWI	GGATC	6 cut(s)	504, 557, 591, 752, 1726, 1739
AcoI	YGGCCR	2 cut(s)	422, 1276
AcsI	RAATTY	1 cut(s)	1254
AcuI	CTGAAG	1 cut(s)	834
AcyI	GRCGYC	2 cut(s)	131, 1692
AfaI	GTAC	8 cut(s)	176, 185, 278, 413, 664, 1082, 1092, 1502
AfeI	AGCGCT	2 cut(s)	26, 1415
AfiI	CCNNNNNNNGG	9 cut(s)	343, 450, 471, 565, 618, 728, 1285, 1531, 1578
AflIII	ACRYGT	1 cut(s)	922
AgsI	TTSAA	5 cut(s)	870, 1012, 1346, 1455, 1740
AjiI	CACGTC	1 cut(s)	972
AjnI	CCWGG	3 cut(s)	192, 464, 798
AleI	CACNNNNGTG	2 cut(s)	1050, 1582
AloI	GAACNNNNNNTCC	2 cut(s)	596, 628
Alw21I	GWGCWC	5 cut(s)	13, 300, 658, 858, 1122
Alw26I	GTCTC	5 cut(s)	313, 356, 748, 988, 1289
Alw44I	GTGCAC	1 cut(s)	654
AlwI	GGATC	6 cut(s)	504, 557, 591, 752, 1726, 1739
AlwNI	CAGNNNCTG	1 cut(s)	161
Ama87I	CYCGRG	3 cut(s)	239, 342, 359
Aor51HI	AGCGCT	2 cut(s)	26, 1415
AoxI	GGCC	8 cut(s)	261, 422, 589, 787, 796, 1152, 1276, 1614
ApaLI	GTGCAC	1 cut(s)	654
ApeKI	GCWGC	2 cut(s)	376, 1411
ApoI	RAATTY	1 cut(s)	1254
Asp700I	GAANNNNTTC	1 cut(s)	1038
AspLEI	GCGC	3 cut(s)	27, 490, 1416
AsuC2I	CCSGG	2 cut(s)	343, 344
AsuHPI	GGTGA	4 cut(s)	102, 108, 709, 1064
AvaI	CYCGRG	3 cut(s)	239, 342, 359
AvaII	GGWCC	6 cut(s)	166, 324, 334, 556, 998, 1627
AxyI	CCTNAGG	1 cut(s)	291
BaeGI	GKGCMC	1 cut(s)	658
BaeI	ACNNNNGTAYC	2 cut(s)	945, 978
BalI	TGGCCA	1 cut(s)	1278
BamHI	GGATCC	1 cut(s)	1731
BanI	GGYRCC	2 cut(s)	121, 209
BanII	GRGCYC	4 cut(s)	13, 300, 1122, 1439
BauI	CACGAG	1 cut(s)	329
Bbv12I	GWGCWC	5 cut(s)	13, 300, 658, 858, 1122
BbvCI	CCTCAGC	1 cut(s)	1200
BbvI	GCAGC	2 cut(s)	363, 1423
BccI	CCATC	4 cut(s)	469, 566, 830, 1236
BciT130I	CCWGG	3 cut(s)	194, 466, 800
BciVI	GTATCC	2 cut(s)	1223, 1582
BclI	TGATCA	1 cut(s)	699
BcnI	CCSGG	2 cut(s)	343, 344
BcoDI	GTCTC	5 cut(s)	313, 356, 748, 988, 1289
BfmI	CTRYAG	4 cut(s)	377, 1060, 1558, 1658
BfoI	RGCGCY	3 cut(s)	28, 491, 1417
BfuAI	ACCTGC	4 cut(s)	637, 685, 1399, 1405
BfuI	GTATCC	2 cut(s)	1223, 1582
BglI	GCCNNNNNGGC	1 cut(s)	795
BglII	AGATCT	1 cut(s)	1357
BisI	GCNGC	4 cut(s)	213, 377, 796, 1412
BlsI	GCNGC	4 cut(s)	214, 378, 797, 1413
Bme1390I	CCNGG	5 cut(s)	194, 343, 344, 466, 800
Bme18I	GGWCC	6 cut(s)	166, 324, 334, 556, 998, 1627
BmeT110I	CYCGRG	3 cut(s)	239, 342, 359
BmgBI	CACGTC	1 cut(s)	972
BmiI	GGNNCC	9 cut(s)	123, 211, 325, 326, 557, 1000, 1315, 1629, 1733
BmrFI	CCNGG	5 cut(s)	194, 343, 344, 466, 800
BmrI	ACTGGG	1 cut(s)	1621
BmsI	GCATC	6 cut(s)	149, 155, 161, 167, 1507, 1573
BmuI	ACTGGG	1 cut(s)	1621
BplI	GAGNNNNNCTC	2 cut(s)	664, 696
BpmI	CTGGAG	2 cut(s)	337, 1234
Bpu10I	CCTNAGC	2 cut(s)	726, 1200
BpuEI	CTTGAG	3 cut(s)	389, 1058, 1444
BpuMI	CCSGG	2 cut(s)	343, 344
BsaAI	YACGTR	1 cut(s)	1593
BsaHI	GRCGYC	2 cut(s)	131, 1692
BsaI	GGTCTC	3 cut(s)	313, 356, 748
BsaJI	CCNNGG	6 cut(s)	192, 342, 465, 747, 790, 799
BsaWI	WCCGGW	3 cut(s)	273, 414, 1728
BsaXI	ACNNNNNCTCC	2 cut(s)	596, 626
Bsc4I	CCNNNNNNNGG	9 cut(s)	343, 450, 471, 565, 618, 728, 1285, 1531, 1578
Bse118I	RCCGGY	2 cut(s)	118, 424
Bse1I	ACTGG	5 cut(s)	320, 670, 1155, 1616, 1685
Bse21I	CCTNAGG	1 cut(s)	291
Bse3DI	GCAATG	1 cut(s)	106
BseBI	CCWGG	3 cut(s)	194, 466, 800
BseDI	CCNNGG	6 cut(s)	192, 342, 465, 747, 790, 799
BseGI	GGATG	7 cut(s)	140, 480, 577, 822, 1036, 1247, 1474
BseLI	CCNNNNNNNGG	9 cut(s)	343, 450, 471, 565, 618, 728, 1285, 1531, 1578
BseMI	GCAATG	1 cut(s)	106
BseMII	CTCAG	4 cut(s)	153, 282, 903, 1191
BseNI	ACTGG	5 cut(s)	320, 670, 1155, 1616, 1685
BseRI	GAGGAG	2 cut(s)	308, 386
BseSI	GKGCMC	1 cut(s)	658
BseX3I	CGGCCG	1 cut(s)	422
BseXI	GCAGC	2 cut(s)	363, 1423
BseYI	CCCAGC	2 cut(s)	470, 545
BsgI	GTGCAG	3 cut(s)	560, 665, 1377
Bsh1285I	CGRYCG	2 cut(s)	425, 429
BshFI	GGCC	8 cut(s)	263, 424, 591, 789, 798, 1154, 1278, 1616
BshNI	GGYRCC	2 cut(s)	121, 209
BsiEI	CGRYCG	2 cut(s)	425, 429
BsiHKAI	GWGCWC	5 cut(s)	13, 300, 658, 858, 1122
BsiHKCI	CYCGRG	3 cut(s)	239, 342, 359
BsiSI	CCGG	6 cut(s)	119, 274, 343, 415, 425, 1729
BslFI	GGGAC	4 cut(s)	337, 427, 984, 1613
BslI	CCNNNNNNNGG	9 cut(s)	343, 450, 471, 565, 618, 728, 1285, 1531, 1578
BsmAI	GTCTC	5 cut(s)	313, 356, 748, 988, 1289
BsmFI	GGGAC	4 cut(s)	337, 427, 984, 1613
BsmI	GAATGC	1 cut(s)	1073
BsnI	GGCC	8 cut(s)	263, 424, 591, 789, 798, 1154, 1278, 1616
Bso31I	GGTCTC	3 cut(s)	313, 356, 748
BsoBI	CYCGRG	3 cut(s)	239, 342, 359
Bsp1286I	GDGCHC	6 cut(s)	13, 300, 658, 858, 1122, 1439
Bsp143I	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
BspACI	CCGC	9 cut(s)	138, 144, 169, 207, 213, 321, 795, 1522, 1653
BspANI	GGCC	8 cut(s)	263, 424, 591, 789, 798, 1154, 1278, 1616
BspCNI	CTCAG	4 cut(s)	154, 283, 904, 1192
BspLI	GGNNCC	9 cut(s)	123, 211, 325, 326, 557, 1000, 1315, 1629, 1733
BspMAI	CTGCAG	2 cut(s)	381, 1662
BspMI	ACCTGC	4 cut(s)	637, 685, 1399, 1405
BspPI	GGATC	6 cut(s)	504, 557, 591, 752, 1726, 1739
BspT107I	GGYRCC	2 cut(s)	121, 209
BspTNI	GGTCTC	3 cut(s)	313, 356, 748
BsrBI	CCGCTC	2 cut(s)	321, 1655
BsrDI	GCAATG	1 cut(s)	106
BsrFI	RCCGGY	2 cut(s)	118, 424
BsrI	ACTGG	5 cut(s)	320, 670, 1155, 1616, 1685
BssAI	RCCGGY	2 cut(s)	118, 424
BssECI	CCNNGG	6 cut(s)	192, 342, 465, 747, 790, 799
BssMI	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
BssNI	GRCGYC	2 cut(s)	131, 1692
BssSI	CACGAG	1 cut(s)	329
BssT1I	CCWWGG	1 cut(s)	747
Bst2BI	CACGAG	1 cut(s)	329
Bst2UI	CCWGG	3 cut(s)	194, 466, 800
Bst6I	CTCTTC	3 cut(s)	521, 1377, 1539
BstACI	GRCGYC	2 cut(s)	131, 1692
BstBAI	YACGTR	1 cut(s)	1593
BstC8I	GCNNGC	7 cut(s)	29, 90, 543, 781, 1426, 1522, 1662
BstDEI	CTNAG	8 cut(s)	77, 162, 291, 726, 912, 1200, 1431, 1670
BstENI	CCTNNNNNAGG	1 cut(s)	616
BstF5I	GGATG	7 cut(s)	140, 480, 577, 822, 1036, 1247, 1474
BstH2I	RGCGCY	3 cut(s)	28, 491, 1417
BstHHI	GCGC	3 cut(s)	27, 490, 1416
BstKTI	GATC	9 cut(s)	499, 552, 586, 702, 760, 1057, 1177, 1360, 1734
BstMAI	GTCTC	5 cut(s)	313, 356, 748, 988, 1289
BstMBI	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
BstMCI	CGRYCG	2 cut(s)	425, 429
BstMWI	GCNNNNNNNGC	7 cut(s)	128, 137, 795, 853, 1434, 1661, 1756
BstNI	CCWGG	3 cut(s)	194, 466, 800
BstNSI	RCATGY	1 cut(s)	926
BstSCI	CCNGG	5 cut(s)	192, 341, 342, 464, 798
BstSFI	CTRYAG	4 cut(s)	377, 1060, 1558, 1658
BstSLI	GKGCMC	1 cut(s)	658
BstSNI	TACGTA	1 cut(s)	1593
BstV1I	GCAGC	2 cut(s)	363, 1423
BstX2I	RGATCY	3 cut(s)	757, 1357, 1731
BstYI	RGATCY	3 cut(s)	757, 1357, 1731
BstZI	CGGCCG	1 cut(s)	422
Bsu36I	CCTNAGG	1 cut(s)	291
BsuI	GTATCC	2 cut(s)	1223, 1582
BsuRI	GGCC	8 cut(s)	263, 424, 591, 789, 798, 1154, 1278, 1616
BtrI	CACGTC	1 cut(s)	972
BtsCI	GGATG	7 cut(s)	140, 480, 577, 822, 1036, 1247, 1474
BtsIMutI	CAGTG	4 cut(s)	15, 1108, 1289, 1609
BveI	ACCTGC	4 cut(s)	637, 685, 1399, 1405
Cac8I	GCNNGC	7 cut(s)	29, 90, 543, 781, 1426, 1522, 1662
CaiI	CAGNNNCTG	1 cut(s)	161
CfoI	GCGC	3 cut(s)	27, 490, 1416
Cfr10I	RCCGGY	2 cut(s)	118, 424
Cfr9I	CCCGGG	1 cut(s)	342
CseI	GACGC	2 cut(s)	120, 1700
Csp6I	GTAC	8 cut(s)	175, 184, 277, 412, 663, 1081, 1091, 1501
CviAII	CATG	9 cut(s)	178, 493, 659, 706, 874, 923, 1441, 1482, 1498
CviQI	GTAC	8 cut(s)	175, 184, 277, 412, 663, 1081, 1091, 1501
DdeI	CTNAG	8 cut(s)	77, 162, 291, 726, 912, 1200, 1431, 1670
DpnI	GATC	9 cut(s)	498, 551, 585, 701, 759, 1056, 1176, 1359, 1733
DpnII	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
EaeI	YGGCCR	2 cut(s)	422, 1276
EagI	CGGCCG	1 cut(s)	422
Eam1104I	CTCTTC	3 cut(s)	521, 1377, 1539
EarI	CTCTTC	3 cut(s)	521, 1377, 1539
Ecl136II	GAGCTC	3 cut(s)	11, 298, 1120
EclXI	CGGCCG	1 cut(s)	422
Eco105I	TACGTA	1 cut(s)	1593
Eco130I	CCWWGG	1 cut(s)	747
Eco24I	GRGCYC	4 cut(s)	13, 300, 1122, 1439
Eco31I	GGTCTC	3 cut(s)	313, 356, 748
Eco47I	GGWCC	6 cut(s)	166, 324, 334, 556, 998, 1627
Eco47III	AGCGCT	2 cut(s)	26, 1415
Eco52I	CGGCCG	1 cut(s)	422
Eco53kI	GAGCTC	3 cut(s)	11, 298, 1120
Eco57I	CTGAAG	1 cut(s)	834
Eco81I	CCTNAGG	1 cut(s)	291
Eco88I	CYCGRG	3 cut(s)	239, 342, 359
EcoICRI	GAGCTC	3 cut(s)	11, 298, 1120
EcoNI	CCTNNNNNAGG	1 cut(s)	616
EcoO109I	RGGNCCY	4 cut(s)	324, 589, 998, 1627
EcoRII	CCWGG	3 cut(s)	192, 464, 798
EcoT14I	CCWWGG	1 cut(s)	747
EcoT38I	GRGCYC	4 cut(s)	13, 300, 1122, 1439
ErhI	CCWWGG	1 cut(s)	747
FaeI	CATG	9 cut(s)	181, 496, 662, 709, 877, 926, 1444, 1485, 1501
FalI	AAGNNNNNCTT	2 cut(s)	1632, 1664
FaqI	GGGAC	4 cut(s)	337, 427, 984, 1613
FatI	CATG	9 cut(s)	177, 492, 658, 705, 873, 922, 1440, 1481, 1497
FauI	CCCGC	2 cut(s)	314, 1529
FbaI	TGATCA	1 cut(s)	699
FblI	GTMKAC	1 cut(s)	1107
Fnu4HI	GCNGC	4 cut(s)	213, 377, 796, 1412
FokI	GGATG	7 cut(s)	127, 487, 584, 809, 1043, 1254, 1481
FriOI	GRGCYC	4 cut(s)	13, 300, 1122, 1439
Fsp4HI	GCNGC	4 cut(s)	213, 377, 796, 1412
GlaI	GCGC	3 cut(s)	26, 489, 1415
GluI	GCNGC	4 cut(s)	213, 377, 796, 1412
GsaI	CCCAGC	2 cut(s)	474, 549
GsuI	CTGGAG	2 cut(s)	337, 1234
HaeII	RGCGCY	3 cut(s)	28, 491, 1417
HaeIII	GGCC	8 cut(s)	263, 424, 591, 789, 798, 1154, 1278, 1616
HapII	CCGG	6 cut(s)	119, 274, 343, 415, 425, 1729
HgaI	GACGC	2 cut(s)	120, 1700
HhaI	GCGC	3 cut(s)	27, 490, 1416
Hin1I	GRCGYC	2 cut(s)	131, 1692
Hin1II	CATG	9 cut(s)	181, 496, 662, 709, 877, 926, 1444, 1485, 1501
Hin6I	GCGC	3 cut(s)	25, 488, 1414
HinP1I	GCGC	3 cut(s)	25, 488, 1414
HincII	GTYRAC	2 cut(s)	835, 1690
HindII	GTYRAC	2 cut(s)	835, 1690
HindIII	AAGCTT	1 cut(s)	1757
HinfI	GANTC	4 cut(s)	48, 418, 1192, 1444
HpaII	CCGG	6 cut(s)	119, 274, 343, 415, 425, 1729
HphI	GGTGA	4 cut(s)	102, 108, 709, 1064
Hpy166II	GTNNAC	5 cut(s)	186, 656, 835, 1108, 1690
Hpy188I	TCNGA	6 cut(s)	163, 684, 913, 1225, 1294, 1774
Hpy188III	TCNNGA	9 cut(s)	239, 337, 761, 886, 985, 1075, 1183, 1213, 1461
Hpy8I	GTNNAC	5 cut(s)	186, 656, 835, 1108, 1690
Hpy99I	CGWCG	1 cut(s)	130
HpyAV	CCTTC	3 cut(s)	238, 253, 1514
HpyCH4IV	ACGT	3 cut(s)	971, 1047, 1592
HpyF10VI	GCNNNNNNNGC	7 cut(s)	128, 137, 795, 853, 1434, 1661, 1756
HpyF3I	CTNAG	8 cut(s)	77, 162, 291, 726, 912, 1200, 1431, 1670
HpySE526I	ACGT	3 cut(s)	971, 1047, 1592
Hsp92I	GRCGYC	2 cut(s)	131, 1692
Hsp92II	CATG	9 cut(s)	181, 496, 662, 709, 877, 926, 1444, 1485, 1501
HspAI	GCGC	3 cut(s)	25, 488, 1414
KflI	GGGWCCC	1 cut(s)	324
Ksp22I	TGATCA	1 cut(s)	699
Kzo9I	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
LmnI	GCTCC	8 cut(s)	36, 295, 303, 318, 373, 622, 844, 1405
Lsp1109I	GCAGC	2 cut(s)	363, 1423
LweI	GCATC	6 cut(s)	149, 155, 161, 167, 1507, 1573
MaeII	ACGT	3 cut(s)	971, 1047, 1592
MaeIII	GTNAC	5 cut(s)	108, 114, 715, 817, 967
MalI	GATC	9 cut(s)	498, 551, 585, 701, 759, 1056, 1176, 1359, 1733
MbiI	CCGCTC	2 cut(s)	321, 1655
MboI	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
MboII	GAAGA	7 cut(s)	262, 535, 538, 890, 1394, 1556, 1590
MfeI	CAATTG	2 cut(s)	563, 735
MflI	RGATCY	3 cut(s)	757, 1357, 1731
MhlI	GDGCHC	6 cut(s)	13, 300, 658, 858, 1122, 1439
MlsI	TGGCCA	1 cut(s)	1278
MluCI	AATT	8 cut(s)	55, 481, 563, 710, 735, 902, 1254, 1715
MluNI	TGGCCA	1 cut(s)	1278
MlyI	GAGTC	1 cut(s)	1453
MmeI	TCCRAC	2 cut(s)	1203, 1532
Mox20I	TGGCCA	1 cut(s)	1278
MroXI	GAANNNNTTC	1 cut(s)	1038
MscI	TGGCCA	1 cut(s)	1278
MseI	TTAA	1 cut(s)	579
MslI	CAYNNNNRTG	3 cut(s)	1050, 1496, 1582
Msp20I	TGGCCA	1 cut(s)	1278
MspA1I	CMGCKG	1 cut(s)	1310
MspI	CCGG	6 cut(s)	119, 274, 343, 415, 425, 1729
MspR9I	CCNGG	5 cut(s)	194, 343, 344, 466, 800
MunI	CAATTG	2 cut(s)	563, 735
Mva1269I	GAATGC	1 cut(s)	1073
MvaI	CCWGG	3 cut(s)	194, 466, 800
MwoI	GCNNNNNNNGC	7 cut(s)	128, 137, 795, 853, 1434, 1661, 1756
NciI	CCSGG	2 cut(s)	343, 344
NdeII	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
NlaIII	CATG	9 cut(s)	181, 496, 662, 709, 877, 926, 1444, 1485, 1501
NlaIV	GGNNCC	9 cut(s)	123, 211, 325, 326, 557, 1000, 1315, 1629, 1733
NmeAIII	GCCGAG	1 cut(s)	771
NmuCI	GTSAC	4 cut(s)	108, 114, 715, 967
NspI	RCATGY	1 cut(s)	926
OliI	CACNNNNGTG	2 cut(s)	1050, 1582
PaqCI	CACCTGC	2 cut(s)	685, 1405
PciI	ACATGT	1 cut(s)	922
PctI	GAATGC	1 cut(s)	1073
PdmI	GAANNNNTTC	1 cut(s)	1038
PfeI	GAWTC	3 cut(s)	48, 418, 1192
PflMI	CCANNNNNTGG	1 cut(s)	565
PkrI	GCNGC	4 cut(s)	214, 378, 797, 1413
PleI	GAGTC	1 cut(s)	1452
PpsI	GAGTC	1 cut(s)	1452
Ppu21I	YACGTR	1 cut(s)	1593
PpuMI	RGGWCCY	3 cut(s)	324, 998, 1627
PscI	ACATGT	1 cut(s)	922
Psp124BI	GAGCTC	3 cut(s)	13, 300, 1122
Psp5II	RGGWCCY	3 cut(s)	324, 998, 1627
Psp6I	CCWGG	3 cut(s)	192, 464, 798
PspFI	CCCAGC	2 cut(s)	470, 545
PspGI	CCWGG	3 cut(s)	192, 464, 798
PspN4I	GGNNCC	9 cut(s)	123, 211, 325, 326, 557, 1000, 1315, 1629, 1733
PspPPI	RGGWCCY	3 cut(s)	324, 998, 1627
PstI	CTGCAG	2 cut(s)	381, 1662
PstNI	CAGNNNCTG	1 cut(s)	161
PsuI	RGATCY	3 cut(s)	757, 1357, 1731
PvuII	CAGCTG	1 cut(s)	1310
RsaI	GTAC	8 cut(s)	176, 185, 278, 413, 664, 1082, 1092, 1502
RsaNI	GTAC	8 cut(s)	175, 184, 277, 412, 663, 1081, 1091, 1501
RseI	CAYNNNNRTG	3 cut(s)	1050, 1496, 1582
SacI	GAGCTC	3 cut(s)	13, 300, 1122
SaqAI	TTAA	1 cut(s)	579
SatI	GCNGC	4 cut(s)	213, 377, 796, 1412
Sau3AI	GATC	9 cut(s)	496, 549, 583, 699, 757, 1054, 1174, 1357, 1731
SchI	GAGTC	1 cut(s)	1453
ScrFI	CCNGG	5 cut(s)	194, 343, 344, 466, 800
SduI	GDGCHC	6 cut(s)	13, 300, 658, 858, 1122, 1439
SfaNI	GCATC	6 cut(s)	149, 155, 161, 167, 1507, 1573
SfcI	CTRYAG	4 cut(s)	377, 1060, 1558, 1658
SfiI	GGCCNNNNNGGCC	1 cut(s)	795
SinI	GGWCC	6 cut(s)	166, 324, 334, 556, 998, 1627
SmaI	CCCGGG	1 cut(s)	344
SmiMI	CAYNNNNRTG	3 cut(s)	1050, 1496, 1582
SmlI	CTYRAG	3 cut(s)	368, 1073, 1459
SmoI	CTYRAG	3 cut(s)	368, 1073, 1459
SnaBI	TACGTA	1 cut(s)	1593
Sse9I	AATT	8 cut(s)	55, 481, 563, 710, 735, 902, 1254, 1715
SsiI	CCGC	9 cut(s)	138, 144, 169, 207, 213, 321, 795, 1522, 1653
SstI	GAGCTC	3 cut(s)	13, 300, 1122
StyD4I	CCNGG	5 cut(s)	192, 341, 342, 464, 798
StyI	CCWWGG	1 cut(s)	747
TaiI	ACGT	3 cut(s)	974, 1050, 1595
TaqI	TCGA	1 cut(s)	986
TasI	AATT	8 cut(s)	55, 481, 563, 710, 735, 902, 1254, 1715
TatI	WGTACW	2 cut(s)	174, 1080
TauI	GCSGC	2 cut(s)	215, 798
TfiI	GAWTC	3 cut(s)	48, 418, 1192
Tru1I	TTAA	1 cut(s)	579
Tru9I	TTAA	1 cut(s)	579
TscAI	CASTG	4 cut(s)	22, 1108, 1289, 1616
TseFI	GTSAC	4 cut(s)	108, 114, 715, 967
TseI	GCWGC	2 cut(s)	376, 1411
Tsp45I	GTSAC	4 cut(s)	108, 114, 715, 967
TspDTI	ATGAA	6 cut(s)	722, 1047, 1266, 1349, 1470, 1620
TspGWI	ACGGA	1 cut(s)	1043
TspMI	CCCGGG	1 cut(s)	342
TspRI	CASTG	4 cut(s)	22, 1108, 1289, 1616
Van91I	CCANNNNNTGG	1 cut(s)	565
VneI	GTGCAC	1 cut(s)	654
VpaK11BI	GGWCC	6 cut(s)	166, 324, 334, 556, 998, 1627
XagI	CCTNNNNNAGG	1 cut(s)	616
XapI	RAATTY	1 cut(s)	1254
XceI	RCATGY	1 cut(s)	926
XcmI	CCANNNNNNNNNTGG	1 cut(s)	472
XmaI	CCCGGG	1 cut(s)	342
XmiI	GTMKAC	1 cut(s)	1107
XmnI	GAANNNNTTC	1 cut(s)	1038

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.