Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0013114

Physical Location & Seq

Genomic Coordinates Reverse (-)

14897284 .. 14903618

Gene Structure 6335 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1074000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1809 bp

ATGCTGAGAGCTCTCACTCTCAGACGCTCCGTCGAGTCCCTCCACCACCACAACCACCTGAGAGCCATAACTCCCACCACCGTCCCGCAAAACCCACTGACCCAAGTAAGCTCCACACGCGCATTATCCACTCTCTCATCGCCGTCATCTCCGGCACCATCTTCCTCATCGTCCGAGCTCCGCAAGTACTTGGGCTACACCGCCCTCGTCCTGTTCAGCGCCGCCGCCACCTACTACTCCTTCCCTTTTCCCGAAAACGCCAAACACAAAAAAGCCCAGATTTTCCGGTATGCTCCGCTGCCGGAGGATCTCCACACCGTCTCCAACTGGAGCGGGACCCACGAGGTCCAGACCCGGGTTTTCCACCAGCCCGAAACGCTTGAGGAGCTGGAAAAGGTTGTGAAGGATGCCCACGAGAAGAAATCCCGGATTCGGCCGGTTGGGTCGGGATTGTCGCCGAATGGGATTGGATTGTCCAGGGCTGGTATGGTCAATCTGGCGCTGATGGATAAGGTTTTGGAGGTGGATAAGGAGAAGAAGAGGGTGAGAGTGCAGGCTGGGATAAGAGTGCAGCAGTTGGTGGATGGGATTAAGGAACATGGGATTACTCTGCAGAACTTTGCCTCCATTAGGGAGCAGCAGATTGGTGGCATTTTGCAGGTTGGTGCACATGGCACTGGTGCAAGATTGCCTCCGATGGATGAGCAGGTGATCAGCATGAAACTGGTCACACCTGCTAAGGGAACAATAGAAGTTTCTAAAGAGAAAGATCCGGAGCTGTTTTATTTAGCCCGCTGTGGCCTTGGTGGCCTTGGAGTGGTTGCTGAAGTCACCATCCAATGTGTTGATAGACAGGAGCTTGTGGAGCACACTACTGTTTCAACCATGGCAGAGATCAAGAAAAATCAAGTCATTGATAAGAAATTGCTATCCGAGAATAGACATGTGAAGTACCTTTACATTCCATATACCGACACTGTTGTGGTTGTGAAATGTAACCCTGTATCAAAATGGAAAGGTCCCCCGAAGTTCACACCCAAATATAGCAGTGATGAAGCCATACAGCACGTTCGAGATCTCTACAGGGAATGCCTCCAGAAATACAGGGTAGTTCCAGATAAGAGTGAAGTAGACATGAATGAGCTCTCATTTACTGAGTTAAGAGATAAACTACTCGCCCTAGATCCTCTCGACAAAGATCACGTTGCAAAGGTTAACCAAGCTGAGGCTGAGTTCTGGAAGAAGTCGGAGGGGTACAGAGTGGGATGGAGTGATGAAATTTTGGGCTTCGATTGTGGTGGTCAACAGTGGGTCTCAGAGACTTGTTTTCCAGCAGGAACCCTAGCCAAACCAAGCATGAAAGACCTTGAATACATAGAAGGTGTAAAGCAGCTCATCGAGAAGAATGAGATACCTGCACCTGCTCCAATAGAGCAGCGCTGGACCGCCTGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCACAAGAGAGGATGATATATTTTCATGGGTTGGTATAATCATGTACCTTCCTACAACAGATCCCCGTCAAAGAAAGGAAATTACAGAAGAGTTTTTTCACTACAGGCGACTGACCCAAACACAATTGTGGGATAAGTATTCCGCGTATGAACACTGGGCTAAGATTGAGGTTCCGAAGGACAAGGATGAACTTGCTGCTCTCCAGGACAGACTAAGAAAGCGATTTCCAGTTGATGCATACAACAAAGCTCGAAGGGAGTTGGACCCTAACAGGATCCTTTCAAATAGCAAGCTGGAAAAGCTTTTCCCATTATCAGATACCATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0008150 GO:0008152 GO:0009536 GO:0016491 GO:0016627 GO:0016632 GO:0016633 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0055114 GO:0080049

KEGG Pathways

Metabolic & Signaling

ko00053 ko01100 ko01110 map00053 map01100 map01110

Pfam Domains

Protein Families

ALO FAD_binding_4

Protein Analysis

603

Amino Acids

68.23

Weight (kDa)

8.65

Isoelectric Point (pI)

46.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FAD_binding_4	PF01565	120 - 250	9.4e-28	FAD binding domain
ALO	PF04030	277 - 594	2.5e-15	D-arabinono-1,4-lactone oxidase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013114)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47930 AT3G47930
fragaria_vesca	FvH4_6g25110
malus_domestica	MD00G1048800.v1.1 MD00G1074000.v1.1
prunus_persica	Prupe.7G046300_v2.0.a1 Prupe.7G046300_v2.0.a1
pyrus_communis	pycom14g07300
rosa_chinensis	RchiOBHm_Chr2g0124941
rosa_laevigata	RLG00000018835
rosa_multiflora	Rmu_sc0008068.1_g000007
rosa_roxburghii	Rroxscaffold_2G00118570 Rroxscaffold_2G00118860
rosa_rugosa	Rorug02G0253600
rosa_samantha	Rh2AG313000 Rh2CG302400 Rh2DG338500
rosa_wichuraiana	Rw2G025280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	697, 742, 1429
Acc36I	ACCTGC	5 cut(s)	649, 697, 742, 1423, 1429
AccB1I	GGYRCC	1 cut(s)	154
AccBSI	CCGCTC	1 cut(s)	333
AccI	GTMKAC	1 cut(s)	1131
AccII	CGCG	2 cut(s)	120, 1625
AccIII	TCCGGA	1 cut(s)	772
AclWI	GGATC	6 cut(s)	315, 764, 1178, 1535, 1750, 1763
AcoI	YGGCCR	1 cut(s)	434
AcsI	RAATTY	1 cut(s)	1278
AcuI	CTGAAG	1 cut(s)	846
AfaI	GTAC	4 cut(s)	188, 953, 1256, 1526
AfeI	AGCGCT	1 cut(s)	1439
AfiI	CCNNNNNNNGG	6 cut(s)	355, 432, 630, 740, 817, 1225
AflIII	ACRYGT	1 cut(s)	943
AgsI	TTSAA	4 cut(s)	882, 1370, 1479, 1764
AhdI	GACNNNNNGTC	1 cut(s)	29
AjnI	CCWGG	2 cut(s)	476, 1683
AleI	CACNNNNGTG	2 cut(s)	980, 1606
AloI	GAACNNNNNNTCC	2 cut(s)	608, 640
Alw21I	GWGCWC	5 cut(s)	13, 180, 670, 870, 1146
Alw26I	GTCTC	3 cut(s)	325, 1313, 1318
Alw44I	GTGCAC	1 cut(s)	666
AlwI	GGATC	6 cut(s)	315, 764, 1178, 1535, 1750, 1763
Ama87I	CYCGRG	1 cut(s)	354
Aor13HI	TCCGGA	1 cut(s)	772
Aor51HI	AGCGCT	1 cut(s)	1439
AoxI	GGCC	3 cut(s)	434, 799, 808
ApaLI	GTGCAC	1 cut(s)	666
ApeKI	GCWGC	6 cut(s)	298, 571, 637, 1390, 1435, 1676
ApoI	RAATTY	1 cut(s)	1278
AspLEI	GCGC	4 cut(s)	122, 221, 502, 1440
AspS9I	GGNCC	5 cut(s)	336, 346, 1019, 1443, 1744
AsuC2I	CCSGG	3 cut(s)	355, 356, 427
AsuHPI	GGTGA	3 cut(s)	556, 721, 823
AvaI	CYCGRG	1 cut(s)	354
AvaII	GGWCC	5 cut(s)	336, 346, 1019, 1443, 1744
BaeGI	GKGCMC	1 cut(s)	670
BamHI	GGATCC	1 cut(s)	1755
BanI	GGYRCC	1 cut(s)	154
BanII	GRGCYC	4 cut(s)	13, 180, 1146, 1463
BarI	GAAGNNNNNNTAC	2 cut(s)	941, 973
BauI	CACGAG	2 cut(s)	341, 413
Bbv12I	GWGCWC	5 cut(s)	13, 180, 670, 870, 1146
BbvCI	CCTCAGC	1 cut(s)	1224
BbvI	GCAGC	6 cut(s)	285, 583, 649, 1402, 1447, 1663
BccI	CCATC	6 cut(s)	166, 499, 578, 691, 842, 1260
BceAI	ACGGC	1 cut(s)	127
BciT130I	CCWGG	2 cut(s)	478, 1685
BclI	TGATCA	1 cut(s)	711
BcnI	CCSGG	3 cut(s)	355, 356, 427
BcoDI	GTCTC	3 cut(s)	325, 1313, 1318
BfaI	CTAG	2 cut(s)	1181, 1343
BfmI	CTRYAG	3 cut(s)	611, 1081, 1582
BfoI	RGCGCY	3 cut(s)	222, 503, 1441
BfuAI	ACCTGC	5 cut(s)	649, 697, 742, 1423, 1429
BglI	GCCNNNNNGGC	1 cut(s)	807
BglII	AGATCT	1 cut(s)	1075
BisI	GCNGC	8 cut(s)	222, 225, 299, 572, 638, 1391, 1436, 1677
BlsI	GCNGC	8 cut(s)	223, 226, 300, 573, 639, 1392, 1437, 1678
BmcAI	AGTACT	1 cut(s)	188
Bme1390I	CCNGG	5 cut(s)	355, 356, 427, 478, 1685
Bme18I	GGWCC	5 cut(s)	336, 346, 1019, 1443, 1744
BmeRI	GACNNNNNGTC	1 cut(s)	29
BmeT110I	CYCGRG	1 cut(s)	354
BmgT120I	GGNCC	5 cut(s)	336, 346, 1019, 1443, 1744
BmiI	GGNNCC	8 cut(s)	156, 337, 338, 1021, 1339, 1653, 1746, 1757
BmrFI	CCNGG	5 cut(s)	355, 356, 427, 478, 1685
BmrI	ACTGGG	1 cut(s)	1645
BmsI	GCATC	2 cut(s)	397, 1705
BmuI	ACTGGG	1 cut(s)	1645
BpmI	CTGGAG	3 cut(s)	349, 1079, 1667
Bpu10I	CCTNAGC	2 cut(s)	738, 1224
BpuEI	CTTGAG	1 cut(s)	401
BpuMI	CCSGG	3 cut(s)	355, 356, 427
BsaI	GGTCTC	1 cut(s)	1318
BsaJI	CCNNGG	5 cut(s)	354, 477, 802, 811, 885
BsaWI	WCCGGW	2 cut(s)	285, 772
BsaXI	ACNNNNNCTCC	4 cut(s)	305, 335, 608, 638
Bsc4I	CCNNNNNNNGG	6 cut(s)	355, 432, 630, 740, 817, 1225
Bse118I	RCCGGY	1 cut(s)	436
Bse1I	ACTGG	5 cut(s)	332, 682, 729, 1640, 1709
BseAI	TCCGGA	1 cut(s)	772
BseBI	CCWGG	2 cut(s)	478, 1685
BseDI	CCNNGG	5 cut(s)	354, 477, 802, 811, 885
BseGI	GGATG	7 cut(s)	412, 589, 706, 834, 1271, 1498, 1672
BseLI	CCNNNNNNNGG	6 cut(s)	355, 432, 630, 740, 817, 1225
BseMII	CTCAG	6 cut(s)	34, 50, 1146, 1215, 1221, 1329
BseNI	ACTGG	5 cut(s)	332, 682, 729, 1640, 1709
BseRI	GAGGAG	1 cut(s)	398
BseSI	GKGCMC	1 cut(s)	670
BseX3I	CGGCCG	1 cut(s)	434
BseXI	GCAGC	6 cut(s)	285, 583, 649, 1402, 1447, 1663
BseYI	CCCAGC	1 cut(s)	557
BsgI	GTGCAG	4 cut(s)	572, 590, 1401, 1435
Bsh1236I	CGCG	2 cut(s)	120, 1625
Bsh1285I	CGRYCG	1 cut(s)	437
BshFI	GGCC	3 cut(s)	436, 801, 810
BshNI	GGYRCC	1 cut(s)	154
BsiEI	CGRYCG	1 cut(s)	437
BsiHKAI	GWGCWC	5 cut(s)	13, 180, 670, 870, 1146
BsiHKCI	CYCGRG	1 cut(s)	354
BsiSI	CCGG	7 cut(s)	152, 286, 302, 355, 427, 437, 773
BslFI	GGGAC	4 cut(s)	22, 68, 349, 1005
BslI	CCNNNNNNNGG	6 cut(s)	355, 432, 630, 740, 817, 1225
BsmAI	GTCTC	3 cut(s)	325, 1313, 1318
BsmBI	CGTCTC	1 cut(s)	325
BsmFI	GGGAC	4 cut(s)	22, 68, 349, 1005
BsmI	GAATGC	1 cut(s)	1094
BsnI	GGCC	3 cut(s)	436, 801, 810
Bso31I	GGTCTC	1 cut(s)	1318
BsoBI	CYCGRG	1 cut(s)	354
Bsp1286I	GDGCHC	6 cut(s)	13, 180, 670, 870, 1146, 1463
Bsp13I	TCCGGA	1 cut(s)	772
Bsp143I	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
Bsp19I	CCATGG	1 cut(s)	885
BspANI	GGCC	3 cut(s)	436, 801, 810
BspCNI	CTCAG	6 cut(s)	33, 51, 1147, 1216, 1222, 1328
BspEI	TCCGGA	1 cut(s)	772
BspFNI	CGCG	2 cut(s)	120, 1625
BspLI	GGNNCC	8 cut(s)	156, 337, 338, 1021, 1339, 1653, 1746, 1757
BspMAI	CTGCAG	1 cut(s)	615
BspMI	ACCTGC	5 cut(s)	649, 697, 742, 1423, 1429
BspPI	GGATC	6 cut(s)	315, 764, 1178, 1535, 1750, 1763
BspT107I	GGYRCC	1 cut(s)	154
BspTNI	GGTCTC	1 cut(s)	1318
BsrBI	CCGCTC	1 cut(s)	333
BsrFI	RCCGGY	1 cut(s)	436
BsrI	ACTGG	5 cut(s)	332, 682, 729, 1640, 1709
BssAI	RCCGGY	1 cut(s)	436
BssECI	CCNNGG	5 cut(s)	354, 477, 802, 811, 885
BssMI	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
BssSI	CACGAG	2 cut(s)	341, 413
BssT1I	CCWWGG	3 cut(s)	802, 811, 885
Bst2BI	CACGAG	2 cut(s)	341, 413
Bst2UI	CCWGG	2 cut(s)	478, 1685
Bst4CI	ACNGT	5 cut(s)	82, 319, 877, 979, 1308
Bst6I	CTCTTC	2 cut(s)	533, 1563
BstC8I	GCNNGC	4 cut(s)	555, 793, 1450, 1772
BstDSI	CCRYGG	1 cut(s)	885
BstENI	CCTNNNNNAGG	1 cut(s)	628
BstF5I	GGATG	7 cut(s)	412, 589, 706, 834, 1271, 1498, 1672
BstFNI	CGCG	2 cut(s)	120, 1625
BstH2I	RGCGCY	3 cut(s)	222, 503, 1441
BstHHI	GCGC	4 cut(s)	122, 221, 502, 1440
BstKTI	GATC	9 cut(s)	310, 714, 772, 897, 1078, 1186, 1201, 1543, 1758
BstMAI	GTCTC	3 cut(s)	325, 1313, 1318
BstMBI	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
BstMCI	CGRYCG	1 cut(s)	437
BstMWI	GCNNNNNNNGC	7 cut(s)	117, 376, 385, 807, 865, 1458, 1780
BstNI	CCWGG	2 cut(s)	478, 1685
BstNSI	RCATGY	1 cut(s)	947
BstSCI	CCNGG	5 cut(s)	353, 354, 425, 476, 1683
BstSFI	CTRYAG	3 cut(s)	611, 1081, 1582
BstSLI	GKGCMC	1 cut(s)	670
BstUI	CGCG	2 cut(s)	120, 1625
BstV1I	GCAGC	6 cut(s)	285, 583, 649, 1402, 1447, 1663
BstX2I	RGATCY	6 cut(s)	307, 769, 1075, 1183, 1540, 1755
BstYI	RGATCY	6 cut(s)	307, 769, 1075, 1183, 1540, 1755
BstZI	CGGCCG	1 cut(s)	434
BsuRI	GGCC	3 cut(s)	436, 801, 810
BtgI	CCRYGG	1 cut(s)	885
BtgZI	GCGATG	1 cut(s)	123
BtsCI	GGATG	7 cut(s)	412, 589, 706, 834, 1271, 1498, 1672
BtsI	GCAGTG	1 cut(s)	1054
BtsIMutI	CAGTG	6 cut(s)	95, 675, 975, 1054, 1313, 1633
BveI	ACCTGC	5 cut(s)	649, 697, 742, 1423, 1429
Cac8I	GCNNGC	4 cut(s)	555, 793, 1450, 1772
CfoI	GCGC	4 cut(s)	122, 221, 502, 1440
Cfr10I	RCCGGY	1 cut(s)	436
Cfr13I	GGNCC	5 cut(s)	336, 346, 1019, 1443, 1744
Cfr9I	CCCGGG	1 cut(s)	354
CseI	GACGC	1 cut(s)	33
Csp6I	GTAC	4 cut(s)	187, 952, 1255, 1525
CviQI	GTAC	4 cut(s)	187, 952, 1255, 1525
DpnI	GATC	9 cut(s)	309, 713, 771, 896, 1077, 1185, 1200, 1542, 1757
DpnII	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
DriI	GACNNNNNGTC	1 cut(s)	29
EaeI	YGGCCR	1 cut(s)	434
EagI	CGGCCG	1 cut(s)	434
Eam1104I	CTCTTC	2 cut(s)	533, 1563
Eam1105I	GACNNNNNGTC	1 cut(s)	29
EarI	CTCTTC	2 cut(s)	533, 1563
Ecl136II	GAGCTC	3 cut(s)	11, 178, 1144
EclXI	CGGCCG	1 cut(s)	434
Eco130I	CCWWGG	3 cut(s)	802, 811, 885
Eco24I	GRGCYC	4 cut(s)	13, 180, 1146, 1463
Eco31I	GGTCTC	1 cut(s)	1318
Eco47I	GGWCC	5 cut(s)	336, 346, 1019, 1443, 1744
Eco47III	AGCGCT	1 cut(s)	1439
Eco52I	CGGCCG	1 cut(s)	434
Eco53kI	GAGCTC	3 cut(s)	11, 178, 1144
Eco57I	CTGAAG	1 cut(s)	846
Eco88I	CYCGRG	1 cut(s)	354
EcoICRI	GAGCTC	3 cut(s)	11, 178, 1144
EcoNI	CCTNNNNNAGG	1 cut(s)	628
EcoO109I	RGGNCCY	2 cut(s)	336, 1019
EcoRII	CCWGG	2 cut(s)	476, 1683
EcoT14I	CCWWGG	3 cut(s)	802, 811, 885
EcoT22I	ATGCAT	1 cut(s)	1720
EcoT38I	GRGCYC	4 cut(s)	13, 180, 1146, 1463
ErhI	CCWWGG	3 cut(s)	802, 811, 885
Esp3I	CGTCTC	1 cut(s)	325
FaqI	GGGAC	4 cut(s)	22, 68, 349, 1005
FauI	CCCGC	3 cut(s)	93, 326, 800
FbaI	TGATCA	1 cut(s)	711
FblI	GTMKAC	1 cut(s)	1131
Fnu4HI	GCNGC	8 cut(s)	222, 225, 299, 572, 638, 1391, 1436, 1677
FokI	GGATG	7 cut(s)	419, 596, 713, 821, 1278, 1505, 1679
FriOI	GRGCYC	4 cut(s)	13, 180, 1146, 1463
Fsp4HI	GCNGC	8 cut(s)	222, 225, 299, 572, 638, 1391, 1436, 1677
FspBI	CTAG	2 cut(s)	1181, 1343
GlaI	GCGC	4 cut(s)	121, 220, 501, 1439
GluI	GCNGC	8 cut(s)	222, 225, 299, 572, 638, 1391, 1436, 1677
GsaI	CCCAGC	1 cut(s)	561
GsuI	CTGGAG	3 cut(s)	349, 1079, 1667
HaeII	RGCGCY	3 cut(s)	222, 503, 1441
HaeIII	GGCC	3 cut(s)	436, 801, 810
HapII	CCGG	7 cut(s)	152, 286, 302, 355, 427, 437, 773
HgaI	GACGC	1 cut(s)	33
HhaI	GCGC	4 cut(s)	122, 221, 502, 1440
Hin6I	GCGC	4 cut(s)	120, 219, 500, 1438
HinP1I	GCGC	4 cut(s)	120, 219, 500, 1438
HincII	GTYRAC	2 cut(s)	1216, 1304
HindII	GTYRAC	2 cut(s)	1216, 1304
HindIII	AAGCTT	1 cut(s)	1781
HinfI	GANTC	3 cut(s)	35, 430, 1468
HpaI	GTTAAC	1 cut(s)	1216
HpaII	CCGG	7 cut(s)	152, 286, 302, 355, 427, 437, 773
HphI	GGTGA	3 cut(s)	556, 721, 823
Hpy166II	GTNNAC	5 cut(s)	668, 1032, 1132, 1216, 1304
Hpy188I	TCNGA	9 cut(s)	23, 175, 696, 934, 1249, 1318, 1656, 1798, 1808
Hpy8I	GTNNAC	5 cut(s)	668, 1032, 1132, 1216, 1304
Hpy99I	CGWCG	1 cut(s)	35
HpyAV	CCTTC	6 cut(s)	250, 397, 1373, 1538, 1651, 1728
HpyCH4III	ACNGT	5 cut(s)	82, 319, 877, 979, 1308
HpyCH4IV	ACGT	2 cut(s)	1068, 1203
HpyF10VI	GCNNNNNNNGC	7 cut(s)	117, 376, 385, 807, 865, 1458, 1780
HpySE526I	ACGT	2 cut(s)	1068, 1203
HspAI	GCGC	4 cut(s)	120, 219, 500, 1438
KflI	GGGWCCC	1 cut(s)	336
Kpn2I	TCCGGA	1 cut(s)	772
Ksp22I	TGATCA	1 cut(s)	711
KspAI	GTTAAC	1 cut(s)	1216
Kzo9I	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
Lsp1109I	GCAGC	6 cut(s)	285, 583, 649, 1402, 1447, 1663
LweI	GCATC	2 cut(s)	397, 1705
MaeI	CTAG	2 cut(s)	1181, 1343
MaeII	ACGT	2 cut(s)	1068, 1203
MaeIII	GTNAC	3 cut(s)	727, 829, 995
MalI	GATC	9 cut(s)	309, 713, 771, 896, 1077, 1185, 1200, 1542, 1757
MbiI	CCGCTC	1 cut(s)	333
MboI	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
MboII	GAAGA	7 cut(s)	153, 430, 547, 550, 1252, 1414, 1580
MfeI	CAATTG	1 cut(s)	1604
MflI	RGATCY	6 cut(s)	307, 769, 1075, 1183, 1540, 1755
MhlI	GDGCHC	6 cut(s)	13, 180, 670, 870, 1146, 1463
MluCI	AATT	4 cut(s)	923, 1278, 1560, 1604
MlyI	GAGTC	2 cut(s)	44, 1477
MmeI	TCCRAC	3 cut(s)	348, 1227, 1722
Mph1103I	ATGCAT	1 cut(s)	1720
MroI	TCCGGA	1 cut(s)	772
MseI	TTAA	3 cut(s)	591, 1160, 1215
MslI	CAYNNNNRTG	2 cut(s)	980, 1606
MspA1I	CMGCKG	2 cut(s)	298, 795
MspI	CCGG	7 cut(s)	152, 286, 302, 355, 427, 437, 773
MspR9I	CCNGG	5 cut(s)	355, 356, 427, 478, 1685
MunI	CAATTG	1 cut(s)	1604
Mva1269I	GAATGC	1 cut(s)	1094
MvaI	CCWGG	2 cut(s)	478, 1685
MvnI	CGCG	2 cut(s)	120, 1625
MwoI	GCNNNNNNNGC	7 cut(s)	117, 376, 385, 807, 865, 1458, 1780
NciI	CCSGG	3 cut(s)	355, 356, 427
NcoI	CCATGG	1 cut(s)	885
NdeII	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
NlaIV	GGNNCC	8 cut(s)	156, 337, 338, 1021, 1339, 1653, 1746, 1757
NmuCI	GTSAC	2 cut(s)	727, 829
NsiI	ATGCAT	1 cut(s)	1720
NspI	RCATGY	1 cut(s)	947
OliI	CACNNNNGTG	2 cut(s)	980, 1606
PaqCI	CACCTGC	3 cut(s)	697, 742, 1429
PciI	ACATGT	1 cut(s)	943
PctI	GAATGC	1 cut(s)	1094
PfeI	GAWTC	1 cut(s)	430
PfoI	TCCNGGA	2 cut(s)	425, 1683
PkrI	GCNGC	8 cut(s)	223, 226, 300, 573, 639, 1392, 1437, 1678
PleI	GAGTC	2 cut(s)	43, 1476
PpsI	GAGTC	2 cut(s)	43, 1476
PpuMI	RGGWCCY	2 cut(s)	336, 1019
PscI	ACATGT	1 cut(s)	943
Psp124BI	GAGCTC	3 cut(s)	13, 180, 1146
Psp5II	RGGWCCY	2 cut(s)	336, 1019
Psp6I	CCWGG	2 cut(s)	476, 1683
PspFI	CCCAGC	1 cut(s)	557
PspGI	CCWGG	2 cut(s)	476, 1683
PspN4I	GGNNCC	8 cut(s)	156, 337, 338, 1021, 1339, 1653, 1746, 1757
PspPI	GGNCC	5 cut(s)	336, 346, 1019, 1443, 1744
PspPPI	RGGWCCY	2 cut(s)	336, 1019
PstI	CTGCAG	1 cut(s)	615
PsuI	RGATCY	6 cut(s)	307, 769, 1075, 1183, 1540, 1755
RsaI	GTAC	4 cut(s)	188, 953, 1256, 1526
RsaNI	GTAC	4 cut(s)	187, 952, 1255, 1525
RseI	CAYNNNNRTG	2 cut(s)	980, 1606
SacI	GAGCTC	3 cut(s)	13, 180, 1146
SaqAI	TTAA	3 cut(s)	591, 1160, 1215
SatI	GCNGC	8 cut(s)	222, 225, 299, 572, 638, 1391, 1436, 1677
Sau3AI	GATC	9 cut(s)	307, 711, 769, 894, 1075, 1183, 1198, 1540, 1755
Sau96I	GGNCC	5 cut(s)	336, 346, 1019, 1443, 1744
ScaI	AGTACT	1 cut(s)	188
SchI	GAGTC	2 cut(s)	44, 1477
ScrFI	CCNGG	5 cut(s)	355, 356, 427, 478, 1685
SduI	GDGCHC	6 cut(s)	13, 180, 670, 870, 1146, 1463
SfaNI	GCATC	2 cut(s)	397, 1705
SfcI	CTRYAG	3 cut(s)	611, 1081, 1582
SfiI	GGCCNNNNNGGCC	1 cut(s)	807
SinI	GGWCC	5 cut(s)	336, 346, 1019, 1443, 1744
SmaI	CCCGGG	1 cut(s)	356
SmiMI	CAYNNNNRTG	2 cut(s)	980, 1606
SmlI	CTYRAG	1 cut(s)	380
SmoI	CTYRAG	1 cut(s)	380
Sse9I	AATT	4 cut(s)	923, 1278, 1560, 1604
SspMI	CTAG	2 cut(s)	1181, 1343
SstI	GAGCTC	3 cut(s)	13, 180, 1146
StyD4I	CCNGG	5 cut(s)	353, 354, 425, 476, 1683
StyI	CCWWGG	3 cut(s)	802, 811, 885
TaaI	ACNGT	5 cut(s)	82, 319, 877, 979, 1308
TaiI	ACGT	2 cut(s)	1071, 1206
TaqI	TCGA	6 cut(s)	33, 1072, 1191, 1290, 1398, 1732
TasI	AATT	4 cut(s)	923, 1278, 1560, 1604
TatI	WGTACW	1 cut(s)	186
TauI	GCSGC	2 cut(s)	224, 227
TfiI	GAWTC	1 cut(s)	430
Tru1I	TTAA	3 cut(s)	591, 1160, 1215
Tru9I	TTAA	3 cut(s)	591, 1160, 1215
TscAI	CASTG	6 cut(s)	102, 682, 982, 1054, 1313, 1640
TseFI	GTSAC	2 cut(s)	727, 829
TseI	GCWGC	6 cut(s)	298, 571, 637, 1390, 1435, 1676
Tsp45I	GTSAC	2 cut(s)	727, 829
TspDTI	ATGAA	8 cut(s)	734, 1068, 1151, 1290, 1373, 1494, 1644, 1683
TspGWI	ACGGA	1 cut(s)	19
TspMI	CCCGGG	1 cut(s)	354
TspRI	CASTG	6 cut(s)	102, 682, 982, 1054, 1313, 1640
VneI	GTGCAC	1 cut(s)	666
VpaK11BI	GGWCC	5 cut(s)	336, 346, 1019, 1443, 1744
XagI	CCTNNNNNAGG	1 cut(s)	628
XapI	RAATTY	1 cut(s)	1278
XceI	RCATGY	1 cut(s)	947
XcmI	CCANNNNNNNNNTGG	1 cut(s)	484
XmaI	CCCGGG	1 cut(s)	354
XmiI	GTMKAC	1 cut(s)	1131
XspI	CTAG	2 cut(s)	1181, 1343
ZrmI	AGTACT	1 cut(s)	188
Zsp2I	ATGCAT	1 cut(s)	1720

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.