pycom14g07300

L-galactono-1,4-lactone dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013114
Physical Location & Seq
Reverse (-)
7363443 .. 7371276
7834 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07300.6

Sequence Viewer

Length: 1929 bp
ATGGGTCTTCTGTCAGGGGTATTGCTGGAAAAAGAAAAATTGGTTTTGCATGTGGGGTTGATTCTTAGTTATGAGAAAAACGAAACGAAAAAAGGGCAAAAGGAAAATTTAAAAAAACAAAACGAAAAAATGCTCAGAGCTCTCACTCTCGGACGCTCCGTCCAGTCCCACCACCACCACAGCAACCTGAGAGCCGTCGCTTCCACCACCGTCCCTCAAAACCCACTGAACCCAGTAAGCTCAACACGCGCATTATCCACCCTTTCATCGCCGTCATCGCCGGCACCATCTTCCTCAGCCTCCGAGCTCCGCAAGTACTTGGGCTACACCGCCCTCGTCCTGTTCAGCGCCGCCGCCACCTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAAAAAGCCCAGATTTTCCGGTATGCTCCGCTGCCGGAGGACCTCCACACAGTCTCCAACTGGAGCGGGACCCACGAGGTCCAAACCAGGGTTTTCCACCAGCCCGAAACGCTTGAGGAGCTGGAGAAGGTCGTGAAGGATGCCCACGAGAAGAAAACCCGGATTCGCCCAGTTGGGTCCGGATTGTCGCCGAATGGGATTGGACTGTCCAGGGCAGGGATGGTCAATTTGGCGCTGATGGATAAGGTTTTGGAGGTGGATAAGGAGAAGAAGAGGGTGAGAGTGCAGGCTGGGATAAGAGTGCAGCAGTTGGTGGATGGGATTAAGGAACATGGGATTACTCTGCAGAACTTTGCCTCCATTAGGGAGCAGCAGATTGGTGGCATTTTGCAGGTTGGTGCACATGGTACTGGTGCAAGATTGCCTCCAATCGATGAGCAGGTAATCAGCATGAAAATGGTCACTCCTGCTAAGGGAACAATAGAAGTTTCTAAAGAGAAAGATCCAGAGCTGTTTTATTTAGCCCGCTGTGGCCTTGGTGGCCTTGGAGTGGTTGCTGAAGTCACCATCCAATGTGTTGATAGACAGGAGCTTGTGGAGCACACTACTGTTTCAACCATGGCAGAGATCAAGAAAAATCATAAGAAATTGCTATCCGAGAATAAACATGTGAAGTACCTTTATATTCCATATACCGACACTGTTGTTGTTGTGAAATGTAACCCTGTATCAAAATGGAAAGGTCCCCCGAAGTTCAAACCCAAATATAGCAGTGATGAATCCATACAGCACGTCCGAGATCTCTACACGGAGTGCCTCCAGAAGTACAGAGTAGTTCCAGATAAGAGTGAAGTAGACATAAATGAGCTCTCATTTACTGAGTTAAGAGATAAACTACTCGCCCTAGATCCTCTCAACAAAGATCACGTTGTAAAGGTTAACCAAGCTGAGGCTGAGTTCTGGAAGAAGTCGGAGGGATACAGAGTGGGATGGAGTGATGAAATTTTGGGTTTTGATTGTGGTGGTCAACAGTGGGTTTCAGAGACATGTTTTCCAGCGGGAACCCTTGCCAAACCAAGCATGAAAGACCTTGAATACATAGAAGGTTTAAAGCAGCTCATTGAGAAGGTTGAGATACCTGCACCTGCCCCAATAGAGCAGCGCTGGACAGCCTGCACTAAGAGCCCCATGAGTCCAGCTTCAAGCACAAGAGAGGAAGATGTATTTTCATGGGTTGGTATAATCATGTACCTTCCTACAACAGATCCCCGCCAAAGAAAGGAAATTACAGAAGAGTTTTTTCACTACAGGCGACTGACCCAGACACAGTTGTGGGATAAGTATTCCGCTTATGAACACTGGGCTAAGATTGAGGTTCCAAAGGACAAGGATGAACTTGCCGCTCTCCAGGACAGACTAAGAAAGCGATTTCCAGTTGATGCGTACAACAAAGCTCGAAGGGAGTTGGACCCTAACAGGATCCTTTCAATTAACAAGCTGGAAAAGCTTTTCCCATTATCAGATTCCATCTGA

Protein Analysis

643

Amino Acids

72.58

Weight (kDa)

8.96

Isoelectric Point (pI)

46.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013114)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47930 AT3G47930
fragaria_vesca FvH4_6g25110
malus_domestica MD00G1048800.v1.1 MD00G1074000.v1.1
prunus_persica Prupe.7G046300_v2.0.a1 Prupe.7G046300_v2.0.a1
pyrus_communis pycom14g07300
rosa_chinensis RchiOBHm_Chr2g0124941
rosa_laevigata RLG00000018835
rosa_multiflora Rmu_sc0008068.1_g000007
rosa_roxburghii Rroxscaffold_2G00118570 Rroxscaffold_2G00118860
rosa_rugosa Rorug02G0253600
rosa_samantha Rh2AG313000 Rh2CG302400 Rh2DG338500
rosa_wichuraiana Rw2G025280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1549
Acc36I ACCTGC 4 cut(s) 778, 826, 1543, 1549
AccB1I GGYRCC 1 cut(s) 283
AccBSI CCGCTC 2 cut(s) 462, 1799
AccI GTMKAC 1 cut(s) 1251
AccII CGCG 1 cut(s) 249
AccIII TCCGGA 1 cut(s) 575
AclWI GGATC 5 cut(s) 893, 1298, 1655, 1870, 1883
AcsI RAATTY 2 cut(s) 106, 1398
AcuI CTGAAG 1 cut(s) 975
AdeI CACNNNGTG 1 cut(s) 1209
AfaI GTAC 6 cut(s) 317, 805, 1073, 1223, 1646, 1841
AfeI AGCGCT 1 cut(s) 1559
AfiI CCNNNNNNNGG 7 cut(s) 484, 612, 759, 869, 946, 1345, 1675
AflIII ACRYGT 2 cut(s) 1063, 1442
AgsI TTSAA 5 cut(s) 1011, 1153, 1490, 1599, 1884
AhdI GACNNNNNGTC 1 cut(s) 158
AjiI CACGTC 1 cut(s) 1189
AjnI CCWGG 3 cut(s) 482, 605, 1803
AleI CACNNNNGTG 1 cut(s) 1726
AloI GAACNNNNNNTCC 2 cut(s) 737, 769
Alw21I GWGCWC 5 cut(s) 142, 309, 799, 999, 1266
Alw26I GTCTC 2 cut(s) 454, 1433
Alw44I GTGCAC 1 cut(s) 795
AlwI GGATC 5 cut(s) 893, 1298, 1655, 1870, 1883
Aor13HI TCCGGA 1 cut(s) 575
Aor51HI AGCGCT 1 cut(s) 1559
AoxI GGCC 2 cut(s) 928, 937
ApaLI GTGCAC 1 cut(s) 795
ApeKI GCWGC 5 cut(s) 427, 700, 766, 1510, 1555
ApoI RAATTY 2 cut(s) 106, 1398
AspLEI GCGC 4 cut(s) 251, 350, 631, 1560
AspS9I GGNCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
AsuC2I CCSGG 1 cut(s) 556
AsuHPI GGTGA 2 cut(s) 685, 952
AvaII GGWCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
BaeGI GKGCMC 1 cut(s) 799
BamHI GGATCC 1 cut(s) 1875
BanI GGYRCC 1 cut(s) 283
BanII GRGCYC 4 cut(s) 142, 309, 1266, 1583
BauI CACGAG 2 cut(s) 470, 542
Bbv12I GWGCWC 5 cut(s) 142, 309, 799, 999, 1266
BbvCI CCTCAGC 2 cut(s) 295, 1344
BbvI GCAGC 5 cut(s) 414, 712, 778, 1522, 1567
BccI CCATC 6 cut(s) 295, 610, 628, 707, 971, 1380
BceAI ACGGC 2 cut(s) 179, 256
BciT130I CCWGG 3 cut(s) 484, 607, 1805
BciVI GTATCC 1 cut(s) 1367
BcnI CCSGG 1 cut(s) 556
BcoDI GTCTC 2 cut(s) 454, 1433
BfaI CTAG 1 cut(s) 1301
BfmI CTRYAG 2 cut(s) 740, 1702
BfoI RGCGCY 3 cut(s) 351, 632, 1561
BfuAI ACCTGC 4 cut(s) 778, 826, 1543, 1549
BfuI GTATCC 1 cut(s) 1367
BglI GCCNNNNNGGC 1 cut(s) 936
BglII AGATCT 1 cut(s) 1195
BisI GCNGC 8 cut(s) 351, 354, 428, 701, 767, 1511, 1556, 1797
BlsI GCNGC 8 cut(s) 352, 355, 429, 702, 768, 1512, 1557, 1798
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 4 cut(s) 484, 556, 607, 1805
Bme18I GGWCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
BmeRI GACNNNNNGTC 1 cut(s) 158
BmgBI CACGTC 1 cut(s) 1189
BmgT120I GGNCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
BmiI GGNNCC 9 cut(s) 285, 466, 467, 574, 1141, 1459, 1773, 1866, 1877
BmrFI CCNGG 4 cut(s) 484, 556, 607, 1805
BmrI ACTGGG 3 cut(s) 227, 560, 1765
BmsI GCATC 2 cut(s) 526, 1825
BmuI ACTGGG 3 cut(s) 227, 560, 1765
BpmI CTGGAG 4 cut(s) 478, 539, 1199, 1787
Bpu10I CCTNAGC 3 cut(s) 295, 867, 1344
BpuEI CTTGAG 1 cut(s) 530
BpuMI CCSGG 1 cut(s) 556
Bsa29I ATCGAT 1 cut(s) 828
BsaJI CCNNGG 5 cut(s) 483, 606, 931, 940, 1014
BsaWI WCCGGW 2 cut(s) 414, 575
BsaXI ACNNNNNCTCC 4 cut(s) 434, 464, 737, 767
Bsc4I CCNNNNNNNGG 7 cut(s) 484, 612, 759, 869, 946, 1345, 1675
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 7 cut(s) 163, 233, 461, 566, 811, 1760, 1829
BseAI TCCGGA 1 cut(s) 575
BseBI CCWGG 3 cut(s) 484, 607, 1805
BseCI ATCGAT 1 cut(s) 828
BseDI CCNNGG 5 cut(s) 483, 606, 931, 940, 1014
BseGI GGATG 6 cut(s) 541, 621, 718, 963, 1391, 1792
BseLI CCNNNNNNNGG 7 cut(s) 484, 612, 759, 869, 946, 1345, 1675
BseMII CTCAG 6 cut(s) 148, 179, 309, 1266, 1335, 1341
BseNI ACTGG 7 cut(s) 163, 233, 461, 566, 811, 1760, 1829
BseRI GAGGAG 1 cut(s) 527
BseSI GKGCMC 1 cut(s) 799
BseXI GCAGC 5 cut(s) 414, 712, 778, 1522, 1567
BseYI CCCAGC 1 cut(s) 686
BsgI GTGCAG 4 cut(s) 701, 719, 1521, 1555
Bsh1236I CGCG 1 cut(s) 249
BshFI GGCC 2 cut(s) 930, 939
BshNI GGYRCC 1 cut(s) 283
BshVI ATCGAT 1 cut(s) 828
BsiHKAI GWGCWC 5 cut(s) 142, 309, 799, 999, 1266
BsiSI CCGG 5 cut(s) 281, 415, 431, 556, 576
BslFI GGGAC 4 cut(s) 151, 197, 478, 1125
BslI CCNNNNNNNGG 7 cut(s) 484, 612, 759, 869, 946, 1345, 1675
BsmAI GTCTC 2 cut(s) 454, 1433
BsmFI GGGAC 4 cut(s) 151, 197, 478, 1125
BsnI GGCC 2 cut(s) 930, 939
Bsp1286I GDGCHC 6 cut(s) 142, 309, 799, 999, 1266, 1583
Bsp13I TCCGGA 1 cut(s) 575
Bsp143I GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
Bsp19I CCATGG 1 cut(s) 1014
BspANI GGCC 2 cut(s) 930, 939
BspCNI CTCAG 6 cut(s) 147, 180, 308, 1267, 1336, 1342
BspDI ATCGAT 1 cut(s) 828
BspEI TCCGGA 1 cut(s) 575
BspFNI CGCG 1 cut(s) 249
BspLI GGNNCC 9 cut(s) 285, 466, 467, 574, 1141, 1459, 1773, 1866, 1877
BspMAI CTGCAG 1 cut(s) 744
BspMI ACCTGC 4 cut(s) 778, 826, 1543, 1549
BspPI GGATC 5 cut(s) 893, 1298, 1655, 1870, 1883
BspT107I GGYRCC 1 cut(s) 283
BsrBI CCGCTC 2 cut(s) 462, 1799
BsrFI RCCGGY 1 cut(s) 280
BsrI ACTGG 7 cut(s) 163, 233, 461, 566, 811, 1760, 1829
BssAI RCCGGY 1 cut(s) 280
BssECI CCNNGG 5 cut(s) 483, 606, 931, 940, 1014
BssMI GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
BssSI CACGAG 2 cut(s) 470, 542
BssT1I CCWWGG 3 cut(s) 931, 940, 1014
Bst2BI CACGAG 2 cut(s) 470, 542
Bst2UI CCWGG 3 cut(s) 484, 607, 1805
Bst4CI ACNGT 7 cut(s) 211, 448, 603, 1006, 1099, 1428, 1725
Bst6I CTCTTC 2 cut(s) 662, 1683
BstC8I GCNNGC 4 cut(s) 282, 684, 922, 1570
BstDSI CCRYGG 1 cut(s) 1014
BstENI CCTNNNNNAGG 1 cut(s) 757
BstF5I GGATG 6 cut(s) 541, 621, 718, 963, 1391, 1792
BstFNI CGCG 1 cut(s) 249
BstH2I RGCGCY 3 cut(s) 351, 632, 1561
BstHHI GCGC 4 cut(s) 251, 350, 631, 1560
BstKTI GATC 7 cut(s) 901, 1026, 1198, 1306, 1321, 1663, 1878
BstMAI GTCTC 2 cut(s) 454, 1433
BstMBI GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
BstMWI GCNNNNNNNGC 8 cut(s) 246, 277, 505, 514, 936, 994, 1578, 1900
BstNI CCWGG 3 cut(s) 484, 607, 1805
BstNSI RCATGY 3 cut(s) 53, 1067, 1446
BstSCI CCNGG 4 cut(s) 482, 554, 605, 1803
BstSFI CTRYAG 2 cut(s) 740, 1702
BstSLI GKGCMC 1 cut(s) 799
BstUI CGCG 1 cut(s) 249
BstV1I GCAGC 5 cut(s) 414, 712, 778, 1522, 1567
BstX2I RGATCY 5 cut(s) 898, 1195, 1303, 1660, 1875
BstYI RGATCY 5 cut(s) 898, 1195, 1303, 1660, 1875
Bsu15I ATCGAT 1 cut(s) 828
BsuI GTATCC 1 cut(s) 1367
BsuRI GGCC 2 cut(s) 930, 939
BsuTUI ATCGAT 1 cut(s) 828
BtgI CCRYGG 1 cut(s) 1014
BtgZI GCGATG 2 cut(s) 252, 261
BtrI CACGTC 1 cut(s) 1189
BtsCI GGATG 6 cut(s) 541, 621, 718, 963, 1391, 1792
BtsI GCAGTG 1 cut(s) 1174
BtsIMutI CAGTG 5 cut(s) 224, 1095, 1174, 1433, 1753
BveI ACCTGC 4 cut(s) 778, 826, 1543, 1549
Cac8I GCNNGC 4 cut(s) 282, 684, 922, 1570
CfoI GCGC 4 cut(s) 251, 350, 631, 1560
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
ClaI ATCGAT 1 cut(s) 828
CseI GACGC 1 cut(s) 162
Csp6I GTAC 6 cut(s) 316, 804, 1072, 1222, 1645, 1840
CviQI GTAC 6 cut(s) 316, 804, 1072, 1222, 1645, 1840
DpnI GATC 7 cut(s) 900, 1025, 1197, 1305, 1320, 1662, 1877
DpnII GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
DraI TTTAAA 2 cut(s) 111, 1506
DraIII CACNNNGTG 1 cut(s) 1209
DriI GACNNNNNGTC 1 cut(s) 158
Eam1104I CTCTTC 2 cut(s) 662, 1683
Eam1105I GACNNNNNGTC 1 cut(s) 158
EarI CTCTTC 2 cut(s) 662, 1683
Ecl136II GAGCTC 3 cut(s) 140, 307, 1264
Eco130I CCWWGG 3 cut(s) 931, 940, 1014
Eco24I GRGCYC 4 cut(s) 142, 309, 1266, 1583
Eco47I GGWCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
Eco47III AGCGCT 1 cut(s) 1559
Eco53kI GAGCTC 3 cut(s) 140, 307, 1264
Eco57I CTGAAG 1 cut(s) 975
EcoICRI GAGCTC 3 cut(s) 140, 307, 1264
EcoNI CCTNNNNNAGG 1 cut(s) 757
EcoO109I RGGNCCY 3 cut(s) 436, 465, 1139
EcoRII CCWGG 3 cut(s) 482, 605, 1803
EcoT14I CCWWGG 3 cut(s) 931, 940, 1014
EcoT38I GRGCYC 4 cut(s) 142, 309, 1266, 1583
ErhI CCWWGG 3 cut(s) 931, 940, 1014
FaqI GGGAC 4 cut(s) 151, 197, 478, 1125
FauI CCCGC 4 cut(s) 455, 929, 1447, 1673
FblI GTMKAC 1 cut(s) 1251
Fnu4HI GCNGC 8 cut(s) 351, 354, 428, 701, 767, 1511, 1556, 1797
FokI GGATG 6 cut(s) 548, 628, 725, 950, 1398, 1799
FriOI GRGCYC 4 cut(s) 142, 309, 1266, 1583
Fsp4HI GCNGC 8 cut(s) 351, 354, 428, 701, 767, 1511, 1556, 1797
FspBI CTAG 1 cut(s) 1301
GlaI GCGC 4 cut(s) 250, 349, 630, 1559
GluI GCNGC 8 cut(s) 351, 354, 428, 701, 767, 1511, 1556, 1797
GsaI CCCAGC 1 cut(s) 690
GsuI CTGGAG 4 cut(s) 478, 539, 1199, 1787
HaeII RGCGCY 3 cut(s) 351, 632, 1561
HaeIII GGCC 2 cut(s) 930, 939
HapII CCGG 5 cut(s) 281, 415, 431, 556, 576
HgaI GACGC 1 cut(s) 162
HhaI GCGC 4 cut(s) 251, 350, 631, 1560
Hin6I GCGC 4 cut(s) 249, 348, 629, 1558
HinP1I GCGC 4 cut(s) 249, 348, 629, 1558
HincII GTYRAC 2 cut(s) 1336, 1424
HindII GTYRAC 2 cut(s) 1336, 1424
HindIII AAGCTT 1 cut(s) 1901
HinfI GANTC 5 cut(s) 61, 559, 1175, 1588, 1919
HpaI GTTAAC 1 cut(s) 1336
HpaII CCGG 5 cut(s) 281, 415, 431, 556, 576
HphI GGTGA 2 cut(s) 685, 952
Hpy166II GTNNAC 4 cut(s) 797, 1252, 1336, 1424
Hpy188I TCNGA 9 cut(s) 137, 152, 304, 1054, 1193, 1369, 1438, 1918, 1928
Hpy188III TCNNGA 7 cut(s) 529, 576, 902, 1027, 1216, 1235, 1357
Hpy8I GTNNAC 4 cut(s) 797, 1252, 1336, 1424
Hpy99I CGWCG 1 cut(s) 200
HpyAV CCTTC 7 cut(s) 379, 517, 526, 1493, 1516, 1658, 1848
HpyCH4III ACNGT 7 cut(s) 211, 448, 603, 1006, 1099, 1428, 1725
HpyCH4IV ACGT 2 cut(s) 1188, 1323
HpyCH4V TGCA 9 cut(s) 49, 682, 700, 742, 787, 797, 812, 1538, 1572
HpyF10VI GCNNNNNNNGC 8 cut(s) 246, 277, 505, 514, 936, 994, 1578, 1900
HpySE526I ACGT 2 cut(s) 1188, 1323
HspAI GCGC 4 cut(s) 249, 348, 629, 1558
KflI GGGWCCC 1 cut(s) 465
Kpn2I TCCGGA 1 cut(s) 575
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
KspAI GTTAAC 1 cut(s) 1336
Kzo9I GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
LmnI GCTCC 8 cut(s) 161, 312, 427, 459, 514, 763, 985, 994
Lsp1109I GCAGC 5 cut(s) 414, 712, 778, 1522, 1567
LweI GCATC 2 cut(s) 526, 1825
MaeI CTAG 1 cut(s) 1301
MaeII ACGT 2 cut(s) 1188, 1323
MaeIII GTNAC 3 cut(s) 856, 958, 1115
MalI GATC 7 cut(s) 900, 1025, 1197, 1305, 1320, 1662, 1877
MbiI CCGCTC 2 cut(s) 462, 1799
MboI GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
MboII GAAGA 7 cut(s) 282, 559, 676, 679, 1372, 1625, 1700
MflI RGATCY 5 cut(s) 898, 1195, 1303, 1660, 1875
MhlI GDGCHC 6 cut(s) 142, 309, 799, 999, 1266, 1583
MluCI AATT 7 cut(s) 38, 106, 622, 1043, 1398, 1680, 1884
MlyI GAGTC 1 cut(s) 1597
MmeI TCCRAC 3 cut(s) 477, 1347, 1842
MroI TCCGGA 1 cut(s) 575
MroNI GCCGGC 1 cut(s) 280
MseI TTAA 6 cut(s) 110, 720, 1280, 1335, 1505, 1887
MslI CAYNNNNRTG 2 cut(s) 851, 1726
MspA1I CMGCKG 3 cut(s) 427, 924, 1454
MspI CCGG 5 cut(s) 281, 415, 431, 556, 576
MspR9I CCNGG 4 cut(s) 484, 556, 607, 1805
MvaI CCWGG 3 cut(s) 484, 607, 1805
MvnI CGCG 1 cut(s) 249
MwoI GCNNNNNNNGC 8 cut(s) 246, 277, 505, 514, 936, 994, 1578, 1900
NaeI GCCGGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 556
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
NgoMIV GCCGGC 1 cut(s) 280
NlaIV GGNNCC 9 cut(s) 285, 466, 467, 574, 1141, 1459, 1773, 1866, 1877
NmuCI GTSAC 2 cut(s) 856, 958
NspI RCATGY 3 cut(s) 53, 1067, 1446
OliI CACNNNNGTG 1 cut(s) 1726
PaqCI CACCTGC 1 cut(s) 1549
PciI ACATGT 2 cut(s) 1063, 1442
PcsI WCGNNNNNNNCGW 1 cut(s) 156
PdiI GCCGGC 1 cut(s) 282
PfeI GAWTC 4 cut(s) 61, 559, 1175, 1919
PfoI TCCNGGA 1 cut(s) 1803
PkrI GCNGC 8 cut(s) 352, 355, 429, 702, 768, 1512, 1557, 1798
PleI GAGTC 1 cut(s) 1596
PpsI GAGTC 1 cut(s) 1596
PpuMI RGGWCCY 3 cut(s) 436, 465, 1139
PscI ACATGT 2 cut(s) 1063, 1442
Psp124BI GAGCTC 3 cut(s) 142, 309, 1266
Psp5II RGGWCCY 3 cut(s) 436, 465, 1139
Psp6I CCWGG 3 cut(s) 482, 605, 1803
PspFI CCCAGC 1 cut(s) 686
PspGI CCWGG 3 cut(s) 482, 605, 1803
PspN4I GGNNCC 9 cut(s) 285, 466, 467, 574, 1141, 1459, 1773, 1866, 1877
PspPI GGNCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
PspPPI RGGWCCY 3 cut(s) 436, 465, 1139
PstI CTGCAG 1 cut(s) 744
PsuI RGATCY 5 cut(s) 898, 1195, 1303, 1660, 1875
RsaI GTAC 6 cut(s) 317, 805, 1073, 1223, 1646, 1841
RsaNI GTAC 6 cut(s) 316, 804, 1072, 1222, 1645, 1840
RseI CAYNNNNRTG 2 cut(s) 851, 1726
SacI GAGCTC 3 cut(s) 142, 309, 1266
SaqAI TTAA 6 cut(s) 110, 720, 1280, 1335, 1505, 1887
SatI GCNGC 8 cut(s) 351, 354, 428, 701, 767, 1511, 1556, 1797
Sau3AI GATC 7 cut(s) 898, 1023, 1195, 1303, 1318, 1660, 1875
Sau96I GGNCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 1597
ScrFI CCNGG 4 cut(s) 484, 556, 607, 1805
SduI GDGCHC 6 cut(s) 142, 309, 799, 999, 1266, 1583
SfaNI GCATC 2 cut(s) 526, 1825
SfcI CTRYAG 2 cut(s) 740, 1702
SfiI GGCCNNNNNGGCC 1 cut(s) 936
SinI GGWCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
SmiMI CAYNNNNRTG 2 cut(s) 851, 1726
SmlI CTYRAG 1 cut(s) 509
SmoI CTYRAG 1 cut(s) 509
Sse9I AATT 7 cut(s) 38, 106, 622, 1043, 1398, 1680, 1884
SspMI CTAG 1 cut(s) 1301
SstI GAGCTC 3 cut(s) 142, 309, 1266
StyD4I CCNGG 4 cut(s) 482, 554, 605, 1803
StyI CCWWGG 3 cut(s) 931, 940, 1014
TaaI ACNGT 7 cut(s) 211, 448, 603, 1006, 1099, 1428, 1725
TaiI ACGT 2 cut(s) 1191, 1326
TaqI TCGA 2 cut(s) 828, 1852
TasI AATT 7 cut(s) 38, 106, 622, 1043, 1398, 1680, 1884
TatI WGTACW 2 cut(s) 315, 1221
TauI GCSGC 3 cut(s) 353, 356, 1799
TfiI GAWTC 4 cut(s) 61, 559, 1175, 1919
Tru1I TTAA 6 cut(s) 110, 720, 1280, 1335, 1505, 1887
Tru9I TTAA 6 cut(s) 110, 720, 1280, 1335, 1505, 1887
TscAI CASTG 5 cut(s) 231, 1102, 1174, 1433, 1760
TseFI GTSAC 2 cut(s) 856, 958
TseI GCWGC 5 cut(s) 427, 700, 766, 1510, 1555
Tsp45I GTSAC 2 cut(s) 856, 958
TspDTI ATGAA 8 cut(s) 255, 863, 1188, 1410, 1493, 1614, 1764, 1803
TspGWI ACGGA 2 cut(s) 148, 1220
TspRI CASTG 5 cut(s) 231, 1102, 1174, 1433, 1760
VneI GTGCAC 1 cut(s) 795
VpaK11BI GGWCC 6 cut(s) 436, 465, 475, 573, 1139, 1864
XagI CCTNNNNNAGG 1 cut(s) 757
XapI RAATTY 2 cut(s) 106, 1398
XceI RCATGY 3 cut(s) 53, 1067, 1446
XcmI CCANNNNNNNNNTGG 1 cut(s) 613
XmiI GTMKAC 1 cut(s) 1251
XspI CTAG 1 cut(s) 1301
ZrmI AGTACT 1 cut(s) 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.