MD00G1051400.v1.1

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012633
Physical Location & Seq
Reverse (-)
9683720 .. 9687529
3810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1051400.v1.1.491

Sequence Viewer

Length: 2034 bp
ATGTCGACTACCGTCGACAAAATGTCCGGCGACCTCCACATGACGCCGGAGCTCCGCAAATCGTTCAACCTCCTCAAGACCCACGCCTCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACCTCGAGGATCACTTCCGCTCCATCGACGATTCCATCCAGTCCGAGCTCGATCAACTGCAAAAACAACAATCGGAAGAGACCCATCTCACTCCGGCTCCGCAATCGCAACCCAAAGAACCCGAATTGAATTTCAAACCCAATTCTGAAGGACCCCAATTGAGTTCCGGTGGCGCCCAATTGAGTTTGAAAGAATCCCAACTCACCGCCACCCTCCAATCATTGAAAACCCAATTCGATTCCGTCGAAACCCAAGTCAAAACCGAAGAGCCTTCGCCGGAAATGTCGACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTATGTGAATGAGCATTTGAAAGAGAGGGATTCGGTGCGTGACGGTGTTTCGGATGCGCTTAAGGTTTCTGTGGATGCTGGGAAGCTGGTGCTTGAGGCATTGAGATGGTTTTATCCATCGGAGTTGAATAAGGGAGGTAAGGAGCTTGATGTCACTGTTACTAGGAAAAGTTGTGTGGTTTTGTTACAAGAGCTGGTGAATGTGAAACCAGTGATTAAACCCGATGTCAGCAGAGAGGCAATGAAGCTGGCATTGGCTTGGAAGGTGAAGGTGATGACTCAAATGTCGAACTCTTTGGAGGTTTGGGCTTTTTTGCAGCTTTTGGATACTTTTCAGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGATGTTTGTTAGTTGCGTAGCGGAGCGGAATTATGCACCACAGTTGTTTAGGTCTCTTGGTTTCACCGATAAGGCCTCTGATCTCATCCAAAAGCTTGTGTCAGAGAATAAGCGCCTTGAGGCTGTTAGATTCAGTCATGCATTTGGACAGTTTGACAAGTTCCCCCCCGTACCCTTGCTTAAAGCACATTTAAAGTTCGTCACCAAAGATGCAAGGACAAGTTCCAAGAGGGGCCAAGATCGTCTTAAAGCACAGGATGAGGCTAGAGACAATGAAATAGTTGCTCTAAGAGGTATAATACGATGCATTGAGGCCTACAAGCTTGTTGTTGAACCACAATATTATCCTGAGACCCTCAGAAAACGCATCAAGCAGCTGAGACGGCAGAGAAAAGAGAGGAAAGCAACTCTAACAGCTCCTGGCCCTGAGGCTCAACCGCAATCCCTGAGTGGAAAGAAACGTACTGCCCCGGCTCAGTCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCCAGCTTTAGCTGCTCAAACTGTTTCTTTCAGAGGAGCAGCCACAGTACGCTCTGTACAACCATCTTACATTCGGCAAGTAAGCTTGTATGACAGTCGAAGGGCAGAATACTTGTTATCATCCGCAGGAATGTCGGGACAAGCATCCACTCCTCCTAATACTTCTTATGGCACCATTACCACCTTGAACCCACTGCGTACATCTCATCATCAGCAAGGAGGCTCGTTCATGGGTCAAGGTGCAGAATACTCTGCTCGATACTACGATTCAGCACACTACGCTCCTGACACTAATAATGACTTATCTGCTCGATCATATGACTTGGTGGGTTCCTCTCCTGGTACCCGTACCCAGCACTTGAACTTGACAGCTCGAGACTATGGATCGACGGCTCCGTCTCATGTACATACGAACTCAGCAATTGCATCTTATGGACTCGGCGGCAGTAGTCATGTAACTTCAGATATAATCCCATTAGTCGCACGATACGGGGCAAGTATAGCTGCAAATGGAAGGACTGGACAGTTTGGATCTGCAGGAAGCCGTACACCTGTGCGCAATGCAAGCCCGAATAGTTACATCCCTTATGCGTATAGGGATCCGGTGAGGGCGCCTAACCACAACGACCGGTCAGTGCCTTCCAGCAGTGGCCATGGCGCGCCGTCTCAGCACCCCCTAGCCATTTATCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

678

Amino Acids

74.79

Weight (kDa)

9.3

Isoelectric Point (pI)

53.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 145 - 410 1.6e-72 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 736, 1732
AbsI CCTCGAGG 1 cut(s) 125
Acc16I TGCGCA 1 cut(s) 1895
Acc36I ACCTGC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 4 cut(s) 293, 1508, 1679, 1948
AccBSI CCGCTC 2 cut(s) 141, 847
AccI GTMKAC 3 cut(s) 5, 15, 407
AccII CGCG 1 cut(s) 1997
AciI CCGC 9 cut(s) 55, 139, 221, 327, 842, 847, 1259, 1461, 1779
AclWI GGATC 5 cut(s) 138, 1729, 1876, 1931, 1944
AcoI YGGCCR 1 cut(s) 1987
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 2 cut(s) 288, 1782
AcyI GRCGYC 4 cut(s) 44, 294, 420, 1949
AfaI GTAC 9 cut(s) 993, 1285, 1386, 1395, 1537, 1681, 1687, 1743, 1885
AfiI CCNNNNNNNGG 2 cut(s) 114, 118
AflII CTTAAG 1 cut(s) 512
AgeI ACCGGT 1 cut(s) 1965
AhdI GACNNNNNGTC 1 cut(s) 22
AjnI CCWGG 2 cut(s) 1240, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw21I GWGCWC 2 cut(s) 54, 171
Alw26I GTCTC 8 cut(s) 194, 879, 1083, 1166, 1195, 1707, 1740, 2007
AlwI GGATC 5 cut(s) 138, 1729, 1876, 1931, 1944
AlwNI CAGNNNCTG 1 cut(s) 1241
Ama87I CYCGRG 2 cut(s) 125, 1710
AoxI GGCC 5 cut(s) 894, 1054, 1134, 1243, 1987
ApeKI GCWGC 5 cut(s) 769, 1195, 1349, 1376, 1841
ApoI RAATTY 1 cut(s) 250
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
AscI GGCGCGCC 1 cut(s) 1995
AsiGI ACCGGT 1 cut(s) 1965
Asp718I GGTACC 1 cut(s) 1679
AspLEI GCGC 7 cut(s) 296, 511, 936, 1896, 1951, 1997, 1999
AspS9I GGNCC 4 cut(s) 121, 272, 1054, 1244
AsuC2I CCSGG 1 cut(s) 1292
AsuHPI GGTGA 9 cut(s) 316, 661, 730, 736, 809, 877, 1015, 1954, 2018
AvaI CYCGRG 2 cut(s) 125, 1710
AvaII GGWCC 2 cut(s) 121, 272
AxyI CCTNAGG 1 cut(s) 1248
BalI TGGCCA 1 cut(s) 1989
BamHI GGATCC 1 cut(s) 1936
BanI GGYRCC 4 cut(s) 293, 1508, 1679, 1948
BanII GRGCYC 2 cut(s) 54, 171
Bbv12I GWGCWC 2 cut(s) 54, 171
BbvI GCAGC 5 cut(s) 781, 1207, 1336, 1388, 1828
BccI CCATC 6 cut(s) 152, 164, 213, 552, 577, 1408
BceAI ACGGC 5 cut(s) 451, 1220, 1743, 1866, 1984
BcgI CGANNNNNNTGC 2 cut(s) 1452, 1486
BciT130I CCWGG 2 cut(s) 1242, 1677
BciVI GTATCC 1 cut(s) 772
BcnI CCSGG 1 cut(s) 1292
BcoDI GTCTC 8 cut(s) 194, 879, 1083, 1166, 1195, 1707, 1740, 2007
BfaI CTAG 3 cut(s) 615, 1086, 2015
BfmI CTRYAG 1 cut(s) 1872
BfoI RGCGCY 3 cut(s) 297, 937, 1952
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 419
BfuI GTATCC 1 cut(s) 772
BisI GCNGC 6 cut(s) 770, 1196, 1350, 1377, 1780, 1842
BlsI GCNGC 6 cut(s) 771, 1197, 1351, 1378, 1781, 1843
Bme1390I CCNGG 3 cut(s) 1242, 1292, 1677
Bme18I GGWCC 2 cut(s) 121, 272
BmeRI GACNNNNNGTC 1 cut(s) 22
BmeT110I CYCGRG 2 cut(s) 125, 1710
BmgT120I GGNCC 4 cut(s) 121, 272, 1054, 1244
BmrFI CCNGG 3 cut(s) 1242, 1292, 1677
BmsI GCATC 8 cut(s) 496, 517, 1021, 1115, 1197, 1333, 1490, 1772
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 1323
BpuEI CTTGAG 3 cut(s) 59, 566, 959
BpuMI CCSGG 1 cut(s) 1292
BsaHI GRCGYC 4 cut(s) 44, 294, 420, 1949
BsaI GGTCTC 3 cut(s) 194, 879, 1166
BsaJI CCNNGG 2 cut(s) 1290, 1990
BsaWI WCCGGW 3 cut(s) 287, 1939, 1965
BsaXI ACNNNNNCTCC 4 cut(s) 125, 155, 202, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 114, 118
Bse118I RCCGGY 1 cut(s) 1965
Bse1I ACTGG 3 cut(s) 160, 662, 1861
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 3 cut(s) 421, 699, 1903
BseBI CCWGG 2 cut(s) 1242, 1677
BseDI CCNNGG 2 cut(s) 1290, 1990
BseGI GGATG 9 cut(s) 156, 511, 532, 906, 1084, 1324, 1457, 1481, 1917
BseLI CCNNNNNNNGG 2 cut(s) 114, 118
BseMI GCAATG 3 cut(s) 421, 699, 1903
BseMII CTCAG 8 cut(s) 1161, 1190, 1192, 1239, 1259, 1310, 1767, 2018
BseNI ACTGG 3 cut(s) 160, 662, 1861
BsePI GCGCGC 1 cut(s) 1995
BseRI GAGGAG 3 cut(s) 62, 1386, 1479
BseXI GCAGC 5 cut(s) 781, 1207, 1336, 1388, 1828
BseYI CCCAGC 2 cut(s) 530, 1689
BsgI GTGCAG 1 cut(s) 1599
Bsh1236I CGCG 1 cut(s) 1997
Bsh1285I CGRYCG 1 cut(s) 1966
BshFI GGCC 5 cut(s) 896, 1056, 1136, 1245, 1989
BshNI GGYRCC 4 cut(s) 293, 1508, 1679, 1948
BshTI ACCGGT 1 cut(s) 1965
BsiEI CGRYCG 1 cut(s) 1966
BsiHKAI GWGCWC 2 cut(s) 54, 171
BsiHKCI CYCGRG 2 cut(s) 125, 1710
BsiSI CCGG 8 cut(s) 27, 47, 215, 288, 398, 1292, 1940, 1966
BslFI GGGAC 2 cut(s) 407, 1488
BslI CCNNNNNNNGG 2 cut(s) 114, 118
BsmAI GTCTC 8 cut(s) 194, 879, 1083, 1166, 1195, 1707, 1740, 2007
BsmBI CGTCTC 3 cut(s) 1195, 1740, 2007
BsmFI GGGAC 2 cut(s) 407, 1488
BsnI GGCC 5 cut(s) 896, 1056, 1136, 1245, 1989
Bso31I GGTCTC 3 cut(s) 194, 879, 1166
BsoBI CYCGRG 2 cut(s) 125, 1710
Bsp1286I GDGCHC 2 cut(s) 54, 171
Bsp1407I TGTACA 2 cut(s) 1393, 1741
Bsp143I GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
Bsp19I CCATGG 1 cut(s) 1990
BspACI CCGC 9 cut(s) 55, 139, 221, 327, 842, 847, 1259, 1461, 1779
BspANI GGCC 5 cut(s) 896, 1056, 1136, 1245, 1989
BspCNI CTCAG 8 cut(s) 1162, 1191, 1191, 1240, 1260, 1309, 1766, 2017
BspFNI CGCG 1 cut(s) 1997
BspMAI CTGCAG 1 cut(s) 1876
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 5 cut(s) 138, 1729, 1876, 1931, 1944
BspQI GCTCTTC 1 cut(s) 381
BspT107I GGYRCC 4 cut(s) 293, 1508, 1679, 1948
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 3 cut(s) 194, 879, 1166
BsrBI CCGCTC 2 cut(s) 141, 847
BsrDI GCAATG 3 cut(s) 421, 699, 1903
BsrFI RCCGGY 1 cut(s) 1965
BsrGI TGTACA 2 cut(s) 1393, 1741
BsrI ACTGG 3 cut(s) 160, 662, 1861
BssAI RCCGGY 1 cut(s) 1965
BssECI CCNNGG 2 cut(s) 1290, 1990
BssHII GCGCGC 1 cut(s) 1995
BssMI GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
BssNI GRCGYC 4 cut(s) 44, 294, 420, 1949
BssT1I CCWWGG 1 cut(s) 1990
Bst2UI CCWGG 2 cut(s) 1242, 1677
Bst6I CTCTTC 2 cut(s) 192, 381
BstACI GRCGYC 4 cut(s) 44, 294, 420, 1949
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 2 cut(s) 1393, 1741
BstC8I GCNNGC 3 cut(s) 702, 1903, 1997
BstDEI CTNAG 9 cut(s) 1109, 1170, 1178, 1199, 1248, 1268, 1296, 1753, 2004
BstDSI CCRYGG 1 cut(s) 1990
BstF5I GGATG 9 cut(s) 156, 511, 532, 906, 1084, 1324, 1457, 1481, 1917
BstFNI CGCG 1 cut(s) 1997
BstH2I RGCGCY 3 cut(s) 297, 937, 1952
BstHHI GCGC 7 cut(s) 296, 511, 936, 1896, 1951, 1997, 1999
BstKTI GATC 8 cut(s) 133, 175, 904, 1063, 1652, 1724, 1871, 1939
BstMAI GTCTC 8 cut(s) 194, 879, 1083, 1166, 1195, 1707, 1740, 2007
BstMBI GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
BstMCI CGRYCG 1 cut(s) 1966
BstMWI GCNNNNNNNGC 6 cut(s) 1204, 1349, 1616, 1838, 1902, 2005
BstNI CCWGG 2 cut(s) 1242, 1677
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 1240, 1290, 1675
BstSFI CTRYAG 1 cut(s) 1872
BstUI CGCG 1 cut(s) 1997
BstV1I GCAGC 5 cut(s) 781, 1207, 1336, 1388, 1828
BstX2I RGATCY 2 cut(s) 1868, 1936
BstYI RGATCY 2 cut(s) 1868, 1936
Bsu36I CCTNAGG 1 cut(s) 1248
BsuI GTATCC 1 cut(s) 772
BsuRI GGCC 5 cut(s) 896, 1056, 1136, 1245, 1989
BtgI CCRYGG 1 cut(s) 1990
BtsCI GGATG 9 cut(s) 156, 511, 532, 906, 1084, 1324, 1457, 1481, 1917
BtsI GCAGTG 2 cut(s) 1529, 1990
BtsIMutI CAGTG 6 cut(s) 606, 669, 814, 1529, 1977, 1990
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 3 cut(s) 702, 1903, 1997
CaiI CAGNNNCTG 1 cut(s) 1241
CfoI GCGC 7 cut(s) 296, 511, 936, 1896, 1951, 1997, 1999
Cfr10I RCCGGY 1 cut(s) 1965
Cfr13I GGNCC 4 cut(s) 121, 272, 1054, 1244
CseI GACGC 2 cut(s) 52, 409
Csp6I GTAC 9 cut(s) 992, 1284, 1385, 1394, 1536, 1680, 1686, 1742, 1884
CspAI ACCGGT 1 cut(s) 1965
CviAII CATG 6 cut(s) 40, 959, 1567, 1739, 1790, 1991
CviQI GTAC 9 cut(s) 992, 1284, 1385, 1394, 1536, 1680, 1686, 1742, 1884
DdeI CTNAG 9 cut(s) 1109, 1170, 1178, 1199, 1248, 1268, 1296, 1753, 2004
DinI GGCGCC 2 cut(s) 295, 1950
DpnI GATC 8 cut(s) 132, 174, 903, 1062, 1651, 1723, 1870, 1938
DpnII GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
DraI TTTAAA 1 cut(s) 1014
DrdI GACNNNNNNGTC 2 cut(s) 736, 1732
DriI GACNNNNNGTC 1 cut(s) 22
DseDI GACNNNNNNGTC 2 cut(s) 736, 1732
EaeI YGGCCR 1 cut(s) 1987
Eam1104I CTCTTC 2 cut(s) 192, 381
Eam1105I GACNNNNNGTC 1 cut(s) 22
EarI CTCTTC 2 cut(s) 192, 381
Ecl136II GAGCTC 2 cut(s) 52, 169
Eco130I CCWWGG 1 cut(s) 1990
Eco147I AGGCCT 2 cut(s) 896, 1136
Eco24I GRGCYC 2 cut(s) 54, 171
Eco31I GGTCTC 3 cut(s) 194, 879, 1166
Eco47I GGWCC 2 cut(s) 121, 272
Eco53kI GAGCTC 2 cut(s) 52, 169
Eco57I CTGAAG 2 cut(s) 288, 1782
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 2 cut(s) 125, 1710
EcoICRI GAGCTC 2 cut(s) 52, 169
EcoO109I RGGNCCY 2 cut(s) 121, 272
EcoRII CCWGG 2 cut(s) 1240, 1675
EcoT14I CCWWGG 1 cut(s) 1990
EcoT22I ATGCAT 2 cut(s) 964, 1130
EcoT38I GRGCYC 2 cut(s) 54, 171
EgeI GGCGCC 2 cut(s) 295, 1950
EheI GGCGCC 2 cut(s) 295, 1950
ErhI CCWWGG 1 cut(s) 1990
Esp3I CGTCTC 3 cut(s) 1195, 1740, 2007
FaeI CATG 6 cut(s) 43, 962, 1570, 1742, 1793, 1994
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 2 cut(s) 407, 1488
FatI CATG 6 cut(s) 39, 958, 1566, 1738, 1789, 1990
FauNDI CATATG 1 cut(s) 1654
FblI GTMKAC 3 cut(s) 5, 15, 407
Fnu4HI GCNGC 6 cut(s) 770, 1196, 1350, 1377, 1780, 1842
FokI GGATG 9 cut(s) 143, 518, 539, 893, 1091, 1311, 1444, 1468, 1904
FriOI GRGCYC 2 cut(s) 54, 171
Fsp4HI GCNGC 6 cut(s) 770, 1196, 1350, 1377, 1780, 1842
FspBI CTAG 3 cut(s) 615, 1086, 2015
FspI TGCGCA 1 cut(s) 1895
GlaI GCGC 7 cut(s) 295, 510, 935, 1895, 1950, 1996, 1998
GluI GCNGC 6 cut(s) 770, 1196, 1350, 1377, 1780, 1842
GsaI CCCAGC 2 cut(s) 534, 1693
GsuI CTGGAG 1 cut(s) 1323
HaeII RGCGCY 3 cut(s) 297, 937, 1952
HaeIII GGCC 5 cut(s) 896, 1056, 1136, 1245, 1989
HapII CCGG 8 cut(s) 27, 47, 215, 288, 398, 1292, 1940, 1966
HgaI GACGC 2 cut(s) 52, 409
HhaI GCGC 7 cut(s) 296, 511, 936, 1896, 1951, 1997, 1999
Hin1I GRCGYC 4 cut(s) 44, 294, 420, 1949
Hin1II CATG 6 cut(s) 43, 962, 1570, 1742, 1793, 1994
Hin6I GCGC 7 cut(s) 294, 509, 934, 1894, 1949, 1995, 1997
HinP1I GCGC 7 cut(s) 294, 509, 934, 1894, 1949, 1995, 1997
HincII GTYRAC 3 cut(s) 6, 16, 408
HindII GTYRAC 3 cut(s) 6, 16, 408
HindIII AAGCTT 3 cut(s) 914, 1142, 1420
HinfI GANTC 8 cut(s) 152, 314, 359, 482, 730, 951, 1604, 1773
HpaII CCGG 8 cut(s) 27, 47, 215, 288, 398, 1292, 1940, 1966
HphI GGTGA 9 cut(s) 316, 661, 730, 736, 809, 877, 1015, 1954, 2018
Hpy166II GTNNAC 4 cut(s) 6, 16, 408, 1886
Hpy188III TCNNGA 6 cut(s) 76, 424, 1169, 1473, 1622, 1712
Hpy8I GTNNAC 4 cut(s) 6, 16, 408, 1886
Hpy99I CGWCG 4 cut(s) 17, 152, 368, 1729
HpyAV CCTTC 7 cut(s) 263, 402, 709, 715, 1431, 1845, 1986
HpyCH4IV ACGT 1 cut(s) 1282
HpyF10VI GCNNNNNNNGC 6 cut(s) 1204, 1349, 1616, 1838, 1902, 2005
HpyF3I CTNAG 9 cut(s) 1109, 1170, 1178, 1199, 1248, 1268, 1296, 1753, 2004
HpySE526I ACGT 1 cut(s) 1282
Hsp92I GRCGYC 4 cut(s) 44, 294, 420, 1949
Hsp92II CATG 6 cut(s) 43, 962, 1570, 1742, 1793, 1994
HspAI GCGC 7 cut(s) 294, 509, 934, 1894, 1949, 1995, 1997
KasI GGCGCC 2 cut(s) 293, 1948
KpnI GGTACC 1 cut(s) 1683
Kzo9I GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
LguI GCTCTTC 1 cut(s) 381
Lsp1109I GCAGC 5 cut(s) 781, 1207, 1336, 1388, 1828
LweI GCATC 8 cut(s) 496, 517, 1021, 1115, 1197, 1333, 1490, 1772
MaeI CTAG 3 cut(s) 615, 1086, 2015
MaeII ACGT 1 cut(s) 1282
MaeIII GTNAC 7 cut(s) 491, 604, 610, 636, 1021, 1792, 1913
MalI GATC 8 cut(s) 132, 174, 903, 1062, 1651, 1723, 1870, 1938
MbiI CCGCTC 2 cut(s) 141, 847
MboI GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
MboII GAAGA 2 cut(s) 209, 398
MfeI CAATTG 3 cut(s) 278, 299, 1758
MflI RGATCY 2 cut(s) 1868, 1936
MhlI GDGCHC 2 cut(s) 54, 171
MlsI TGGCCA 1 cut(s) 1989
MluCI AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 850, 1758
MluNI TGGCCA 1 cut(s) 1989
Mly113I GGCGCC 2 cut(s) 294, 1949
MlyI GAGTC 2 cut(s) 724, 1767
Mox20I TGGCCA 1 cut(s) 1989
Mph1103I ATGCAT 2 cut(s) 964, 1130
MscI TGGCCA 1 cut(s) 1989
MseI TTAA 5 cut(s) 513, 669, 1002, 1013, 1068
MslI CAYNNNNRTG 1 cut(s) 556
Msp20I TGGCCA 1 cut(s) 1989
MspA1I CMGCKG 1 cut(s) 1198
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 8 cut(s) 27, 47, 215, 288, 398, 1292, 1940, 1966
MspR9I CCNGG 3 cut(s) 1242, 1292, 1677
MunI CAATTG 3 cut(s) 278, 299, 1758
MvaI CCWGG 2 cut(s) 1242, 1677
MvnI CGCG 1 cut(s) 1997
MwoI GCNNNNNNNGC 6 cut(s) 1204, 1349, 1616, 1838, 1902, 2005
NarI GGCGCC 2 cut(s) 294, 1949
NciI CCSGG 1 cut(s) 1292
NcoI CCATGG 1 cut(s) 1990
NdeI CATATG 1 cut(s) 1654
NdeII GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
NlaIII CATG 6 cut(s) 43, 962, 1570, 1742, 1793, 1994
NmeAIII GCCGAG 1 cut(s) 1755
NmuCI GTSAC 3 cut(s) 491, 604, 1021
NsbI TGCGCA 1 cut(s) 1895
NsiI ATGCAT 2 cut(s) 964, 1130
PaeR7I CTCGAG 2 cut(s) 125, 1710
PalAI GGCGCGCC 1 cut(s) 1995
PauI GCGCGC 1 cut(s) 1995
PceI AGGCCT 2 cut(s) 896, 1136
PciSI GCTCTTC 1 cut(s) 381
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 1730
PfeI GAWTC 6 cut(s) 152, 314, 359, 482, 951, 1604
PinAI ACCGGT 1 cut(s) 1965
PkrI GCNGC 6 cut(s) 771, 1197, 1351, 1378, 1781, 1843
PleI GAGTC 2 cut(s) 724, 1767
PluTI GGCGCC 2 cut(s) 297, 1952
PpsI GAGTC 2 cut(s) 724, 1767
PpuMI RGGWCCY 2 cut(s) 121, 272
PshAI GACNNNNGTC 1 cut(s) 11
Psp124BI GAGCTC 2 cut(s) 54, 171
Psp5II RGGWCCY 2 cut(s) 121, 272
Psp6I CCWGG 2 cut(s) 1240, 1675
PspFI CCCAGC 2 cut(s) 530, 1689
PspGI CCWGG 2 cut(s) 1240, 1675
PspPI GGNCC 4 cut(s) 121, 272, 1054, 1244
PspPPI RGGWCCY 2 cut(s) 121, 272
PspXI VCTCGAGB 1 cut(s) 125
PstI CTGCAG 1 cut(s) 1876
PstNI CAGNNNCTG 1 cut(s) 1241
PsuI RGATCY 2 cut(s) 1868, 1936
PteI GCGCGC 1 cut(s) 1995
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 9 cut(s) 993, 1285, 1386, 1395, 1537, 1681, 1687, 1743, 1885
RsaNI GTAC 9 cut(s) 992, 1284, 1385, 1394, 1536, 1680, 1686, 1742, 1884
RseI CAYNNNNRTG 1 cut(s) 556
SacI GAGCTC 2 cut(s) 54, 171
SalI GTCGAC 3 cut(s) 4, 14, 406
SapI GCTCTTC 1 cut(s) 381
SaqAI TTAA 5 cut(s) 513, 669, 1002, 1013, 1068
SatI GCNGC 6 cut(s) 770, 1196, 1350, 1377, 1780, 1842
Sau3AI GATC 8 cut(s) 130, 172, 901, 1060, 1649, 1721, 1868, 1936
Sau96I GGNCC 4 cut(s) 121, 272, 1054, 1244
SchI GAGTC 2 cut(s) 724, 1767
ScrFI CCNGG 3 cut(s) 1242, 1292, 1677
SduI GDGCHC 2 cut(s) 54, 171
SfaNI GCATC 8 cut(s) 496, 517, 1021, 1115, 1197, 1333, 1490, 1772
SfcI CTRYAG 1 cut(s) 1872
SfoI GGCGCC 2 cut(s) 295, 1950
Sfr274I CTCGAG 2 cut(s) 125, 1710
SgsI GGCGCGCC 1 cut(s) 1995
SinI GGWCC 2 cut(s) 121, 272
SlaI CTCGAG 2 cut(s) 125, 1710
SmiMI CAYNNNNRTG 1 cut(s) 556
SmlI CTYRAG 6 cut(s) 74, 125, 512, 545, 938, 1710
SmoI CTYRAG 6 cut(s) 74, 125, 512, 545, 938, 1710
Sse9I AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 850, 1758
SseBI AGGCCT 2 cut(s) 896, 1136
SsiI CCGC 9 cut(s) 55, 139, 221, 327, 842, 847, 1259, 1461, 1779
SspDI GGCGCC 2 cut(s) 293, 1948
SspI AATATT 1 cut(s) 1163
SspMI CTAG 3 cut(s) 615, 1086, 2015
SstI GAGCTC 2 cut(s) 54, 171
StuI AGGCCT 2 cut(s) 896, 1136
StyD4I CCNGG 3 cut(s) 1240, 1290, 1675
StyI CCWWGG 1 cut(s) 1990
TaiI ACGT 1 cut(s) 1285
TasI AATT 8 cut(s) 245, 250, 262, 278, 299, 353, 850, 1758
TatI WGTACW 2 cut(s) 1393, 1741
TauI GCSGC 1 cut(s) 1782
TfiI GAWTC 6 cut(s) 152, 314, 359, 482, 951, 1604
Tru1I TTAA 5 cut(s) 513, 669, 1002, 1013, 1068
Tru9I TTAA 5 cut(s) 513, 669, 1002, 1013, 1068
TscAI CASTG 6 cut(s) 613, 669, 814, 1536, 1977, 1990
TseFI GTSAC 3 cut(s) 491, 604, 1021
TseI GCWGC 5 cut(s) 769, 1195, 1349, 1376, 1841
Tsp45I GTSAC 3 cut(s) 491, 604, 1021
TspDTI ATGAA 3 cut(s) 710, 1110, 1555
TspGWI ACGGA 2 cut(s) 352, 1722
TspRI CASTG 6 cut(s) 613, 669, 814, 1536, 1977, 1990
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 2 cut(s) 121, 272
XapI RAATTY 1 cut(s) 250
XhoI CTCGAG 2 cut(s) 125, 1710
XmiI GTMKAC 3 cut(s) 5, 15, 407
XspI CTAG 3 cut(s) 615, 1086, 2015
Zsp2I ATGCAT 2 cut(s) 964, 1130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.