MD12G1232000.v1.1

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012633
Physical Location & Seq
Forward (+)
30666458 .. 30669027
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1232000.v1.1.491

Sequence Viewer

Length: 2019 bp
ATGTCGACCACCGTCGACAAAATCTCGGGCGACCTTCACATGACGCCGGAGCTACGCAAATCCTTCAACCTCCTCAAGACCCACGCCTCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACCTCGAGGAGCACTTCCGCTCCATCGACAATTCCATCCAATCCAAGCTCGACCAACTCCAAAAACAACAATCGGAAGAGGCCCATGTCGCTCCGGCTCCGCAATCGCAACCCAAAGAACCCGAATTGAATTTTAAACCCAATTCCGAAGAACCCCAATTGGGCTCCGGTGACGCCCAATTGAGTTCGAAAGAATCCCAAGTCACCGCCACCCTCCAATCCTTGAAAACCCAATTCGATTCCGTCGAAACCCAAGTCAAAACCGACGAGCCTTTGCCGGAAATGTCGACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTATGTGAACGAGCATTTGAAAGAGAGGGGTTTGGTGCGTGACGGTGTTGCGAATGCGCTTAAGGCTTCTGTGGATGCTGGGAAGCTAGTGCTCGAGGCAGTGAAATGGTTTTATCCATCAGAATCGAATATGGGAGGTAAGGAGCTTGACGTCACTGTTACTAGGAAAAGTTGTGCGATTTTGTTACAAGCGCTGGTGAATGTGAAACCAGTGATTAAACCCGATATCATCAGAGAGGCAATGAAGCTTGCATTGGCTTGGAAGGCAAAGGTGATAACTCAAATGTCGAACTCTTCGGAGGTTTGGGCTTTTTTTCAGCTTCTGGATGCTTTTGGGATGGGTGGTGAGTTTGACAGTGATGAGATTTTGAAGCTTTTTAGTTGCGTAGCAGATCAGAATTCTGCACCGGAGTTGTTTAGGTCCCTTGGTTTCGCCGATAAAGCCTCTGATTTCATCCAAAAGCTTATATCAGAGAATAAGCGGGTTGATGCTGTTAGATTCAGTCGTGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCATCACGAAAGATGCAAGGACTAGTTCCGAGAGGGGGCAAGACAGTCTTAAAGCACAGGATGAGTCCATAGACAAAGAAATAGCTGCTCTAAGAGGCATAAAACATTGCGTTGAGAACTACAACCTTGTTGTTGAACCAGAATATTATCCCAAGACCCTCGGAAAACGCATTAGGCAGCTGAAAAGGGGGAGAAAAGAGAGGAAAGCAACTCTAAGAGCTCTTGGCCCTAAGGCTCAGCCGCAACCCCTGAGTGGAAAGAAACGTACTGCCCCAGATCAGCCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCCAGCTTTAGCTGCTCAAACTGTTTCTCTCAGAGGACCAGCCACAGTACACGCTATACAACCATCTTATATTCGCCAAGAAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGCTATCGTCGGCTGGAATGCCCCGACAAGCAACCACCCCGCCCAGTACTTCTTATGGCACCATTTCCACCTTGAACCCACTGCGAGCATCTCATCATCAGCAAGGAGGCTCATTCATCGGTCAAGGTGCGGAATACCCTTCTCGATACTATGATTCAGCACACTACGCTCCTGATACTATTAATAACTTATCTGCTCGATCATATGATTTGGTGGGTTCCTCTCCTGGTACCCGTGGCCAGCTCGTGAACTTAACAGCTCGAGACTATGGATTGACGGGTCCGTCTCATGTACATACTAACCCAGAACTTGGGGGCAGTGGTCGTGTAACTTCAGATCTGAGTCCATTAGTCGCACGATACAGAACAAGTATACCTGCAAATGGAAGGACTGGACAGTTAGGATCAGCAGGAAGCCCTACACCGATGCGCATTGCAAGCCCGAACAGTTCCATCCCTTATGCCTATAGGGATCCGCTTAGGGCGCCTAACTACAATGACCGGGCGGTGCCTTCCAGCAGTGGCTATGGCGCGCCGTCTCAGCACCCCCCAGGCATTTATCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

673

Amino Acids

73.77

Weight (kDa)

9.28

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 145 - 409 2.6e-67 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1732
AatII GACGTC 1 cut(s) 606
AbsI CCTCGAGG 1 cut(s) 125
Acc16I TGCGCA 1 cut(s) 1880
Acc36I ACCTGC 2 cut(s) 419, 1834
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 4 cut(s) 1508, 1679, 1933, 1957
AccB7I CCANNNNNTGG 1 cut(s) 1760
AccBSI CCGCTC 1 cut(s) 141
AccI GTMKAC 4 cut(s) 5, 15, 407, 1822
AccII CGCG 1 cut(s) 1982
AciI CCGC 9 cut(s) 139, 221, 327, 934, 1259, 1490, 1580, 1925, 1955
AclWI GGATC 3 cut(s) 1861, 1916, 1929
AcoI YGGCCR 1 cut(s) 1687
AcsI RAATTY 2 cut(s) 250, 850
AcuI CTGAAG 1 cut(s) 1767
AcyI GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
AfaI GTAC 6 cut(s) 993, 1285, 1386, 1498, 1681, 1743
AfeI AGCGCT 1 cut(s) 645
AfiI CCNNNNNNNGG 7 cut(s) 114, 118, 281, 1053, 1271, 1760, 1832
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 7 cut(s) 67, 250, 346, 472, 823, 1154, 1525
AhlI ACTAGT 1 cut(s) 1040
AjnI CCWGG 2 cut(s) 1675, 1999
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw21I GWGCWC 3 cut(s) 135, 546, 1240
Alw26I GTCTC 3 cut(s) 1707, 1740, 1992
AlwI GGATC 3 cut(s) 1861, 1916, 1929
AlwNI CAGNNNCTG 1 cut(s) 775
Ama87I CYCGRG 4 cut(s) 25, 125, 545, 1710
Aor51HI AGCGCT 1 cut(s) 645
AoxI GGCC 3 cut(s) 201, 1243, 1687
ApeKI GCWGC 3 cut(s) 1103, 1195, 1349
ApoI RAATTY 2 cut(s) 250, 850
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
AscI GGCGCGCC 1 cut(s) 1980
AseI ATTAAT 1 cut(s) 1632
Asp718I GGTACC 1 cut(s) 1679
AspLEI GCGC 6 cut(s) 511, 646, 1881, 1936, 1982, 1984
AspS9I GGNCC 6 cut(s) 121, 202, 873, 1244, 1373, 1730
AsuC2I CCSGG 1 cut(s) 1952
AsuHPI GGTGA 6 cut(s) 302, 316, 661, 736, 809, 2003
AsuII TTCGAA 1 cut(s) 308
AvaI CYCGRG 4 cut(s) 25, 125, 545, 1710
AvaII GGWCC 4 cut(s) 121, 873, 1373, 1730
AxyI CCTNAGG 1 cut(s) 1248
BalI TGGCCA 1 cut(s) 1689
BamHI GGATCC 1 cut(s) 1921
BanI GGYRCC 4 cut(s) 1508, 1679, 1933, 1957
BanII GRGCYC 2 cut(s) 287, 1240
BauI CACGAG 1 cut(s) 1694
Bbv12I GWGCWC 3 cut(s) 135, 546, 1240
BbvI GCAGC 3 cut(s) 1090, 1207, 1336
BccI CCATC 6 cut(s) 152, 164, 577, 784, 1408, 1910
BceAI ACGGC 2 cut(s) 451, 1969
BciT130I CCWGG 2 cut(s) 1677, 2001
BcnI CCSGG 1 cut(s) 1952
BcoDI GTCTC 3 cut(s) 1707, 1740, 1992
BcuI ACTAGT 1 cut(s) 1040
BfaI CTAG 3 cut(s) 539, 615, 1041
BfmI CTRYAG 1 cut(s) 1915
BfoI RGCGCY 2 cut(s) 647, 1937
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 2 cut(s) 419, 1834
BglII AGATCT 1 cut(s) 1786
BisI GCNGC 4 cut(s) 1104, 1196, 1259, 1350
BlpI GCTNAGC 1 cut(s) 1254
BlsI GCNGC 4 cut(s) 1105, 1197, 1260, 1351
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 3 cut(s) 1677, 1952, 2001
Bme18I GGWCC 4 cut(s) 121, 873, 1373, 1730
BmeT110I CYCGRG 4 cut(s) 25, 125, 545, 1710
BmgT120I GGNCC 6 cut(s) 121, 202, 873, 1244, 1373, 1730
BmrFI CCNGG 3 cut(s) 1677, 1952, 2001
BmrI ACTGGG 1 cut(s) 1488
BmsI GCATC 7 cut(s) 517, 769, 931, 1021, 1333, 1547, 1866
BmuI ACTGGG 1 cut(s) 1488
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 1323
Bpu10I CCTNAGC 1 cut(s) 1928
Bpu1102I GCTNAGC 1 cut(s) 1254
Bpu14I TTCGAA 1 cut(s) 308
BpuEI CTTGAG 1 cut(s) 59
BpuMI CCSGG 1 cut(s) 1952
BsaHI GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
BsaJI CCNNGG 4 cut(s) 877, 1177, 1684, 1999
BsaWI WCCGGW 2 cut(s) 287, 859
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 7 cut(s) 114, 118, 281, 1053, 1271, 1760, 1832
Bse1I ACTGG 3 cut(s) 662, 1494, 1846
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 4 cut(s) 421, 699, 1123, 1881
BseBI CCWGG 2 cut(s) 1677, 2001
BseDI CCNNGG 4 cut(s) 877, 1177, 1684, 1999
BseGI GGATG 8 cut(s) 156, 532, 784, 795, 906, 1084, 1324, 1902
BseLI CCNNNNNNNGG 7 cut(s) 114, 118, 281, 1053, 1271, 1760, 1832
BseMI GCAATG 4 cut(s) 421, 699, 1123, 1881
BseMII CTCAG 5 cut(s) 1259, 1268, 1381, 1781, 2003
BseNI ACTGG 3 cut(s) 662, 1494, 1846
BsePI GCGCGC 1 cut(s) 1980
BseRI GAGGAG 2 cut(s) 62, 143
BseXI GCAGC 3 cut(s) 1090, 1207, 1336
BseYI CCCAGC 1 cut(s) 530
BsgI GTGCAG 2 cut(s) 840, 1461
Bsh1236I CGCG 1 cut(s) 1982
BshFI GGCC 3 cut(s) 203, 1245, 1689
BshNI GGYRCC 4 cut(s) 1508, 1679, 1933, 1957
BsiHKAI GWGCWC 3 cut(s) 135, 546, 1240
BsiHKCI CYCGRG 4 cut(s) 25, 125, 545, 1710
BsiSI CCGG 6 cut(s) 47, 215, 288, 398, 860, 1951
BslFI GGGAC 2 cut(s) 407, 859
BslI CCNNNNNNNGG 7 cut(s) 114, 118, 281, 1053, 1271, 1760, 1832
BsmAI GTCTC 3 cut(s) 1707, 1740, 1992
BsmBI CGTCTC 2 cut(s) 1740, 1992
BsmFI GGGAC 2 cut(s) 407, 859
BsmI GAATGC 2 cut(s) 511, 1473
BsnI GGCC 3 cut(s) 203, 1245, 1689
BsoBI CYCGRG 4 cut(s) 25, 125, 545, 1710
Bsp119I TTCGAA 1 cut(s) 308
Bsp1286I GDGCHC 4 cut(s) 135, 287, 546, 1240
Bsp1407I TGTACA 1 cut(s) 1741
Bsp143I GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
Bsp1720I GCTNAGC 1 cut(s) 1254
BspACI CCGC 9 cut(s) 139, 221, 327, 934, 1259, 1490, 1580, 1925, 1955
BspANI GGCC 3 cut(s) 203, 1245, 1689
BspCNI CTCAG 5 cut(s) 1260, 1267, 1380, 1782, 2002
BspFNI CGCG 1 cut(s) 1982
BspMI ACCTGC 2 cut(s) 419, 1834
BspPI GGATC 3 cut(s) 1861, 1916, 1929
BspT104I TTCGAA 1 cut(s) 308
BspT107I GGYRCC 4 cut(s) 1508, 1679, 1933, 1957
BspTI CTTAAG 1 cut(s) 512
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 4 cut(s) 421, 699, 1123, 1881
BsrGI TGTACA 1 cut(s) 1741
BsrI ACTGG 3 cut(s) 662, 1494, 1846
BssECI CCNNGG 4 cut(s) 877, 1177, 1684, 1999
BssHII GCGCGC 1 cut(s) 1980
BssMI GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
BssNAI GTATAC 1 cut(s) 1823
BssNI GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
BssSI CACGAG 1 cut(s) 1694
BssT1I CCWWGG 1 cut(s) 877
Bst1107I GTATAC 1 cut(s) 1823
Bst2BI CACGAG 1 cut(s) 1694
Bst2UI CCWGG 2 cut(s) 1677, 2001
Bst6I CTCTTC 2 cut(s) 192, 751
BstACI GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
BstAFI CTTAAG 1 cut(s) 512
BstAUI TGTACA 1 cut(s) 1741
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 5 cut(s) 702, 1536, 1691, 1888, 1982
BstDEI CTNAG 9 cut(s) 1109, 1232, 1248, 1254, 1268, 1367, 1790, 1928, 1989
BstDSI CCRYGG 1 cut(s) 1684
BstF5I GGATG 8 cut(s) 156, 532, 784, 795, 906, 1084, 1324, 1902
BstFNI CGCG 1 cut(s) 1982
BstH2I RGCGCY 2 cut(s) 647, 1937
BstHHI GCGC 6 cut(s) 511, 646, 1881, 1936, 1982, 1984
BstKTI GATC 6 cut(s) 847, 1297, 1652, 1789, 1856, 1924
BstMAI GTCTC 3 cut(s) 1707, 1740, 1992
BstMBI GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
BstNI CCWGG 2 cut(s) 1677, 2001
BstPAI GACNNNNGTC 1 cut(s) 11
BstSCI CCNGG 3 cut(s) 1675, 1950, 1999
BstSFI CTRYAG 1 cut(s) 1915
BstUI CGCG 1 cut(s) 1982
BstV1I GCAGC 3 cut(s) 1090, 1207, 1336
BstX2I RGATCY 2 cut(s) 1786, 1921
BstYI RGATCY 2 cut(s) 1786, 1921
BstZ17I GTATAC 1 cut(s) 1823
Bsu36I CCTNAGG 1 cut(s) 1248
BsuRI GGCC 3 cut(s) 203, 1245, 1689
BtgI CCRYGG 1 cut(s) 1684
BtsCI GGATG 8 cut(s) 156, 532, 784, 795, 906, 1084, 1324, 1902
BtsI GCAGTG 4 cut(s) 558, 1529, 1774, 1975
BtsIMutI CAGTG 7 cut(s) 558, 606, 669, 814, 1529, 1774, 1975
BveI ACCTGC 2 cut(s) 419, 1834
Cac8I GCNNGC 5 cut(s) 702, 1536, 1691, 1888, 1982
CaiI CAGNNNCTG 1 cut(s) 775
CfoI GCGC 6 cut(s) 511, 646, 1881, 1936, 1982, 1984
Cfr13I GGNCC 6 cut(s) 121, 202, 873, 1244, 1373, 1730
CseI GACGC 3 cut(s) 52, 302, 409
Csp6I GTAC 6 cut(s) 992, 1284, 1385, 1497, 1680, 1742
CviAII CATG 3 cut(s) 40, 206, 1739
CviQI GTAC 6 cut(s) 992, 1284, 1385, 1497, 1680, 1742
DdeI CTNAG 9 cut(s) 1109, 1232, 1248, 1254, 1268, 1367, 1790, 1928, 1989
DinI GGCGCC 1 cut(s) 1935
DpnI GATC 6 cut(s) 846, 1296, 1651, 1788, 1855, 1923
DpnII GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
DraI TTTAAA 2 cut(s) 256, 1014
DrdI GACNNNNNNGTC 1 cut(s) 1732
DseDI GACNNNNNNGTC 1 cut(s) 1732
EaeI YGGCCR 1 cut(s) 1687
Eam1104I CTCTTC 2 cut(s) 192, 751
EarI CTCTTC 2 cut(s) 192, 751
Ecl136II GAGCTC 1 cut(s) 1238
Eco130I CCWWGG 1 cut(s) 877
Eco24I GRGCYC 2 cut(s) 287, 1240
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 4 cut(s) 121, 873, 1373, 1730
Eco47III AGCGCT 1 cut(s) 645
Eco53kI GAGCTC 1 cut(s) 1238
Eco57I CTGAAG 1 cut(s) 1767
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 4 cut(s) 25, 125, 545, 1710
EcoICRI GAGCTC 1 cut(s) 1238
EcoO109I RGGNCCY 2 cut(s) 121, 873
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 2 cut(s) 1675, 1999
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 877
EcoT38I GRGCYC 2 cut(s) 287, 1240
EgeI GGCGCC 1 cut(s) 1935
EheI GGCGCC 1 cut(s) 1935
ErhI CCWWGG 1 cut(s) 877
Esp3I CGTCTC 2 cut(s) 1740, 1992
FaeI CATG 3 cut(s) 43, 209, 1742
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 2 cut(s) 407, 859
FatI CATG 3 cut(s) 39, 205, 1738
FauI CCCGC 2 cut(s) 927, 1497
FauNDI CATATG 1 cut(s) 1654
FblI GTMKAC 4 cut(s) 5, 15, 407, 1822
Fnu4HI GCNGC 4 cut(s) 1104, 1196, 1259, 1350
FokI GGATG 8 cut(s) 143, 539, 791, 802, 893, 1091, 1311, 1889
FriOI GRGCYC 2 cut(s) 287, 1240
Fsp4HI GCNGC 4 cut(s) 1104, 1196, 1259, 1350
FspAI RTGCGCAY 1 cut(s) 1880
FspBI CTAG 3 cut(s) 539, 615, 1041
FspI TGCGCA 1 cut(s) 1880
GlaI GCGC 6 cut(s) 510, 645, 1880, 1935, 1981, 1983
GluI GCNGC 4 cut(s) 1104, 1196, 1259, 1350
GsaI CCCAGC 1 cut(s) 534
GsuI CTGGAG 1 cut(s) 1323
HaeII RGCGCY 2 cut(s) 647, 1937
HaeIII GGCC 3 cut(s) 203, 1245, 1689
HapII CCGG 6 cut(s) 47, 215, 288, 398, 860, 1951
HgaI GACGC 3 cut(s) 52, 302, 409
HhaI GCGC 6 cut(s) 511, 646, 1881, 1936, 1982, 1984
Hin1I GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
Hin1II CATG 3 cut(s) 43, 209, 1742
Hin6I GCGC 6 cut(s) 509, 644, 1879, 1934, 1980, 1982
HinP1I GCGC 6 cut(s) 509, 644, 1879, 1934, 1980, 1982
HincII GTYRAC 3 cut(s) 6, 16, 408
HindII GTYRAC 3 cut(s) 6, 16, 408
HindIII AAGCTT 4 cut(s) 698, 824, 914, 1420
HinfI GANTC 7 cut(s) 314, 359, 575, 951, 1082, 1604, 1792
HpaII CCGG 6 cut(s) 47, 215, 288, 398, 860, 1951
HphI GGTGA 6 cut(s) 302, 316, 661, 736, 809, 2003
Hpy166II GTNNAC 7 cut(s) 6, 16, 408, 460, 1387, 1699, 1823
Hpy188III TCNNGA 8 cut(s) 76, 424, 776, 1024, 1593, 1622, 1696, 1712
Hpy8I GTNNAC 7 cut(s) 6, 16, 408, 460, 1387, 1699, 1823
Hpy99I CGWCG 4 cut(s) 17, 368, 389, 1462
HpyAV CCTTC 6 cut(s) 44, 73, 709, 1599, 1830, 1971
HpyCH4IV ACGT 2 cut(s) 603, 1282
HpyCH4V TGCA 8 cut(s) 414, 704, 857, 962, 1034, 1442, 1829, 1886
HpyF3I CTNAG 9 cut(s) 1109, 1232, 1248, 1254, 1268, 1367, 1790, 1928, 1989
HpySE526I ACGT 2 cut(s) 603, 1282
Hsp92I GRCGYC 5 cut(s) 44, 294, 420, 603, 1934
Hsp92II CATG 3 cut(s) 43, 209, 1742
HspAI GCGC 6 cut(s) 509, 644, 1879, 1934, 1980, 1982
KasI GGCGCC 1 cut(s) 1933
KpnI GGTACC 1 cut(s) 1683
Kzo9I GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
LmnI GCTCC 9 cut(s) 49, 130, 146, 217, 223, 290, 595, 1342, 1624
Lsp1109I GCAGC 3 cut(s) 1090, 1207, 1336
LweI GCATC 7 cut(s) 517, 769, 931, 1021, 1333, 1547, 1866
MaeI CTAG 3 cut(s) 539, 615, 1041
MaeII ACGT 2 cut(s) 603, 1282
MaeIII GTNAC 7 cut(s) 290, 322, 491, 604, 610, 636, 1777
MalI GATC 6 cut(s) 846, 1296, 1651, 1788, 1855, 1923
MbiI CCGCTC 1 cut(s) 141
MboI GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
MboII GAAGA 3 cut(s) 209, 281, 738
MfeI CAATTG 2 cut(s) 278, 299
MflI RGATCY 2 cut(s) 1786, 1921
MhlI GDGCHC 4 cut(s) 135, 287, 546, 1240
MlsI TGGCCA 1 cut(s) 1689
MluCI AATT 8 cut(s) 151, 245, 250, 262, 278, 299, 353, 850
MluNI TGGCCA 1 cut(s) 1689
Mly113I GGCGCC 1 cut(s) 1934
MlyI GAGTC 2 cut(s) 1091, 1801
Mox20I TGGCCA 1 cut(s) 1689
MscI TGGCCA 1 cut(s) 1689
MseI TTAA 8 cut(s) 255, 513, 669, 1002, 1013, 1068, 1632, 1703
Msp20I TGGCCA 1 cut(s) 1689
MspA1I CMGCKG 1 cut(s) 1198
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 6 cut(s) 47, 215, 288, 398, 860, 1951
MspR9I CCNGG 3 cut(s) 1677, 1952, 2001
MunI CAATTG 2 cut(s) 278, 299
Mva1269I GAATGC 2 cut(s) 511, 1473
MvaI CCWGG 2 cut(s) 1677, 2001
MvnI CGCG 1 cut(s) 1982
NarI GGCGCC 1 cut(s) 1934
NciI CCSGG 1 cut(s) 1952
NdeI CATATG 1 cut(s) 1654
NdeII GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
NlaIII CATG 3 cut(s) 43, 209, 1742
NmuCI GTSAC 4 cut(s) 290, 322, 491, 604
NsbI TGCGCA 1 cut(s) 1880
NspV TTCGAA 1 cut(s) 308
PaeR7I CTCGAG 3 cut(s) 125, 545, 1710
PalAI GGCGCGCC 1 cut(s) 1980
PauI GCGCGC 1 cut(s) 1980
PcsI WCGNNNNNNNCGW 1 cut(s) 363
PctI GAATGC 2 cut(s) 511, 1473
PfeI GAWTC 5 cut(s) 314, 359, 575, 951, 1604
PflMI CCANNNNNTGG 1 cut(s) 1760
PkrI GCNGC 4 cut(s) 1105, 1197, 1260, 1351
PleI GAGTC 2 cut(s) 1090, 1800
PluTI GGCGCC 1 cut(s) 1937
PpsI GAGTC 2 cut(s) 1090, 1800
PpuMI RGGWCCY 2 cut(s) 121, 873
PshAI GACNNNNGTC 1 cut(s) 11
PshBI ATTAAT 1 cut(s) 1632
Psp124BI GAGCTC 1 cut(s) 1240
Psp5II RGGWCCY 2 cut(s) 121, 873
Psp6I CCWGG 2 cut(s) 1675, 1999
PspFI CCCAGC 1 cut(s) 530
PspGI CCWGG 2 cut(s) 1675, 1999
PspPI GGNCC 6 cut(s) 121, 202, 873, 1244, 1373, 1730
PspPPI RGGWCCY 2 cut(s) 121, 873
PspXI VCTCGAGB 2 cut(s) 125, 545
PstNI CAGNNNCTG 1 cut(s) 775
PsuI RGATCY 2 cut(s) 1786, 1921
PteI GCGCGC 1 cut(s) 1980
PvuII CAGCTG 1 cut(s) 1198
RsaI GTAC 6 cut(s) 993, 1285, 1386, 1498, 1681, 1743
RsaNI GTAC 6 cut(s) 992, 1284, 1385, 1497, 1680, 1742
SacI GAGCTC 1 cut(s) 1240
SalI GTCGAC 3 cut(s) 4, 14, 406
SaqAI TTAA 8 cut(s) 255, 513, 669, 1002, 1013, 1068, 1632, 1703
SatI GCNGC 4 cut(s) 1104, 1196, 1259, 1350
Sau3AI GATC 6 cut(s) 844, 1294, 1649, 1786, 1853, 1921
Sau96I GGNCC 6 cut(s) 121, 202, 873, 1244, 1373, 1730
ScaI AGTACT 1 cut(s) 1498
SchI GAGTC 2 cut(s) 1091, 1801
ScrFI CCNGG 3 cut(s) 1677, 1952, 2001
SduI GDGCHC 4 cut(s) 135, 287, 546, 1240
SfaNI GCATC 7 cut(s) 517, 769, 931, 1021, 1333, 1547, 1866
SfcI CTRYAG 1 cut(s) 1915
SfoI GGCGCC 1 cut(s) 1935
Sfr274I CTCGAG 3 cut(s) 125, 545, 1710
SfuI TTCGAA 1 cut(s) 308
SgsI GGCGCGCC 1 cut(s) 1980
SinI GGWCC 4 cut(s) 121, 873, 1373, 1730
SlaI CTCGAG 3 cut(s) 125, 545, 1710
SmlI CTYRAG 5 cut(s) 74, 125, 512, 545, 1710
SmoI CTYRAG 5 cut(s) 74, 125, 512, 545, 1710
SpeI ACTAGT 1 cut(s) 1040
Sse9I AATT 8 cut(s) 151, 245, 250, 262, 278, 299, 353, 850
SsiI CCGC 9 cut(s) 139, 221, 327, 934, 1259, 1490, 1580, 1925, 1955
SspDI GGCGCC 1 cut(s) 1933
SspI AATATT 1 cut(s) 1163
SspMI CTAG 3 cut(s) 539, 615, 1041
SstI GAGCTC 1 cut(s) 1240
StyD4I CCNGG 3 cut(s) 1675, 1950, 1999
StyI CCWWGG 1 cut(s) 877
TaiI ACGT 2 cut(s) 606, 1285
TaqII GACCGA 1 cut(s) 1559
TasI AATT 8 cut(s) 151, 245, 250, 262, 278, 299, 353, 850
TatI WGTACW 3 cut(s) 1384, 1496, 1741
TauI GCSGC 1 cut(s) 1261
TfiI GAWTC 5 cut(s) 314, 359, 575, 951, 1604
Tru1I TTAA 8 cut(s) 255, 513, 669, 1002, 1013, 1068, 1632, 1703
Tru9I TTAA 8 cut(s) 255, 513, 669, 1002, 1013, 1068, 1632, 1703
TscAI CASTG 7 cut(s) 558, 613, 669, 814, 1536, 1774, 1975
TseFI GTSAC 4 cut(s) 290, 322, 491, 604
TseI GCWGC 3 cut(s) 1103, 1195, 1349
Tsp45I GTSAC 4 cut(s) 290, 322, 491, 604
TspDTI ATGAA 4 cut(s) 710, 895, 1009, 1555
TspGWI ACGGA 2 cut(s) 352, 1722
TspRI CASTG 7 cut(s) 558, 613, 669, 814, 1536, 1774, 1975
Van91I CCANNNNNTGG 1 cut(s) 1760
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 4 cut(s) 121, 873, 1373, 1730
VspI ATTAAT 1 cut(s) 1632
XapI RAATTY 2 cut(s) 250, 850
XhoI CTCGAG 3 cut(s) 125, 545, 1710
XmiI GTMKAC 4 cut(s) 5, 15, 407, 1822
XspI CTAG 3 cut(s) 539, 615, 1041
ZraI GACGTC 1 cut(s) 604
ZrmI AGTACT 1 cut(s) 1498
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.