Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0012633

Physical Location & Seq

Genomic Coordinates Forward (+)

22611696 .. 22614259

Gene Structure 2564 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g21440.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2019 bp

ATGTCGACCACCGTCGACAAAATTTCCGGTGACCTTCACATGAGGCCGGAGCTACGCAAATCCTTCAACCTCCTCAAGACCCACGCCTCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACCTCGAGGAGCACTTCCGCTCCATCGACAATTCCATCCAATCCAAGCTCGACCAACTGCAAAAACAACAATCGGAAGAGACCCATCTCGCTCCGGCTCCGCAATCGCAACCCAGAGAACCCGAATTGAATTTTAAACCCAATTCCGAAGAACCCCAATTGGGTTCCGGTGACGCCCAATTGAGTTCGAAAGAATCCCAAGTCACCGCCACCCTCCAGTCCTTTAAAACCCAATTCGATTCCGTCGAAATCCAAGTCAAAACCGACGAGCCTTTGCCAGAAATGTCGACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTACGTGAATGAGCATTTGAAAGAGCGGGGTTCGGTGCGTGACGGTGTTGCGGATGCGCTTAAGGTTTCTGTGGATGCTGGGAGGCTAGTGCTCGAGGCAGTGAAATGGTTTTATCCATCAGAATCGAATAAGGGAGGTAAGGAGCTTGACGTCACTGTTACTAGGAAAAGTTGTGCGATTTTGTTACAAGGGCTGGTGAATGTGAAACCAGTGATTAAACCCGATATCATCAGAGAGGCAATGAAGCTTGCATTGGCTTGGAAGGCAAAGGTGATAACTCAAATGTCCAACTCTTTGGAGGTTTGGGCTTTTTTTCAGCTTCTGGATGCTTTTGGGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGAAGCTTTTTAGTTGCATAGCAGATCGGAATTCTGCACCGGAGTTGTTTAGGTCTCTTGGTTTCGCCGATAAAGCCTCTGATTTCATCCAAAAGCTTGTATCAGAGAATAAGCGGCTTGACGCTGTTAGATTCAGTCATGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCATCACGAAAGATGCAAGGACAAGTTCCAAGAGGGGGCAAGACCGTCTTAAAGCACAGGATGAGGCCATAGACAAAGAAATAGCTGCTCTAAGAGGCATAATACGTTGCGTTGAGGACTACAAGCTTGTCGTTGAACCAGAATATTCTCCTGAGATCCTTGGAAAACACATTAGGCAGCTCAGAAGGGAGAGAAAAGAGAGGAAAGCAACTCTAACAGCTCCTGGCCCTAAGGCTCAGCCGCAACCCCTGAGTGGTAAGAAACGTACTGCCCCAGGTCAGCCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCGAGCTTTAACTGCTCAAACTGTTTCTTTCAGAGGACCAGCCACAGTACGCGCTATACAACCATCTTATATTCGCCAAGTAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGTTATCGTCGGCTGGAATGCCCAGACAAGCAACCACTCCGCCCAGTACTTCTTATGGCACCATTTCCACCTTGAACCCACTGCGAGCATCTCATCATGAGCAAGGAGGCTCATTCATTGGTCAAGGTGCAGAATACCCTTCTCGGTACTATGATTCAGCACACTACGCTCCTGATACTAATAATAACTTATCTGCTCGATCGTATGTCTTGGTGGGTTCCTCTCCTGGTACCCGTGGCCAGCTCGTGAACTTAACAGCTCAAGACTATGGATTGACAGGTCCGTCTCATGTTCATACTAACTCAGAACTTGGGGGCAGTGGTCATGTAACTTCAGATCTGAGCCCATTAGTCGCACGATACAGAACAAGTATAGCTGCAAATGGAAGGACTGGACAGTTAGGATCAGCAGGAAGCCCTACACCAGTGCGCGTTGCAAGCCCGAACAGTTCCATCCCTTATGCCTATAGGGATCCACTTAGGGCGCCTAACTACAATGACCGGGCAGTGCGTTCCAAGAGTGGCTATGGCGCGCCATCTCAGCACCCCCCAGCCATTTATCACCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

673

Amino Acids

74.03

Weight (kDa)

9.42

Isoelectric Point (pI)

48.23

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04240 FvH4_6g04240
malus_domestica	MD00G1051400.v1.1 MD12G1231700.v1.1 MD12G1232000.v1.1
prunus_persica	Prupe.6G334900_v2.0.a1
pyrus_communis	pycom04g19180 pycom12g21440
rosa_chinensis	RchiOBHm_Chr3g0452911
rosa_laevigata	RLG00000025560
rosa_multiflora	Rmu_sc0002823.1_g000005 Rmu_sc0002823.1_g000006
rosa_roxburghii	Rroxscaffold_6G00429920
rosa_rugosa	Rorug02G0643600
rosa_samantha	Rh3AG046400 Rh3BG048900 Rh3CG047500 Rh3DG048500
rosa_wichuraiana	Rw3G003650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1732
AatII	GACGTC	1 cut(s)	606
AbsI	CCTCGAGG	1 cut(s)	125
Acc36I	ACCTGC	1 cut(s)	419
Acc65I	GGTACC	1 cut(s)	1679
AccB1I	GGYRCC	3 cut(s)	1508, 1679, 1933
AccBSI	CCGCTC	2 cut(s)	141, 478
AccI	GTMKAC	3 cut(s)	5, 15, 407
AccII	CGCG	3 cut(s)	1389, 1881, 1982
AciI	CCGC	8 cut(s)	139, 221, 327, 478, 503, 934, 1259, 1490
AclWI	GGATC	4 cut(s)	1168, 1861, 1916, 1929
AcoI	YGGCCR	1 cut(s)	1687
AcsI	RAATTY	3 cut(s)	21, 250, 850
AcuI	CTGAAG	1 cut(s)	1767
AcyI	GRCGYC	4 cut(s)	294, 420, 603, 1934
AfaI	GTAC	7 cut(s)	455, 993, 1285, 1386, 1498, 1598, 1681
AfiI	CCNNNNNNNGG	5 cut(s)	114, 118, 281, 1053, 1271
AflII	CTTAAG	1 cut(s)	512
AgsI	TTSAA	6 cut(s)	67, 250, 472, 823, 1154, 1525
AjnI	CCWGG	3 cut(s)	1240, 1291, 1675
AjuI	GAANNNNNNNTTGG	2 cut(s)	689, 721
Alw21I	GWGCWC	2 cut(s)	135, 546
Alw26I	GTCTC	3 cut(s)	194, 879, 1740
AlwI	GGATC	4 cut(s)	1168, 1861, 1916, 1929
AlwNI	CAGNNNCTG	2 cut(s)	775, 1241
Ama87I	CYCGRG	3 cut(s)	125, 545, 1338
AoxI	GGCC	4 cut(s)	44, 1083, 1243, 1687
ApeKI	GCWGC	3 cut(s)	1103, 1195, 1826
ApoI	RAATTY	3 cut(s)	21, 250, 850
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
AscI	GGCGCGCC	1 cut(s)	1980
Asp718I	GGTACC	1 cut(s)	1679
AspLEI	GCGC	6 cut(s)	511, 1391, 1881, 1936, 1982, 1984
AspS9I	GGNCC	4 cut(s)	121, 1244, 1373, 1730
AsuC2I	CCSGG	1 cut(s)	1952
AsuHPI	GGTGA	7 cut(s)	41, 302, 316, 661, 736, 809, 2003
AsuII	TTCGAA	1 cut(s)	308
AvaI	CYCGRG	3 cut(s)	125, 545, 1338
AvaII	GGWCC	3 cut(s)	121, 1373, 1730
AxyI	CCTNAGG	1 cut(s)	1248
BalI	TGGCCA	1 cut(s)	1689
BamHI	GGATCC	1 cut(s)	1921
BanI	GGYRCC	3 cut(s)	1508, 1679, 1933
BanII	GRGCYC	1 cut(s)	1796
BauI	CACGAG	1 cut(s)	1694
Bbv12I	GWGCWC	2 cut(s)	135, 546
BbvI	GCAGC	3 cut(s)	1090, 1207, 1813
BccI	CCATC	7 cut(s)	152, 164, 213, 577, 1408, 1910, 1993
BceAI	ACGGC	1 cut(s)	451
BciT130I	CCWGG	3 cut(s)	1242, 1293, 1677
BcnI	CCSGG	1 cut(s)	1952
BcoDI	GTCTC	3 cut(s)	194, 879, 1740
BfaI	CTAG	2 cut(s)	539, 615
BfmI	CTRYAG	1 cut(s)	1915
BfoI	RGCGCY	1 cut(s)	1937
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	1 cut(s)	419
BglII	AGATCT	1 cut(s)	1786
BisI	GCNGC	5 cut(s)	935, 1104, 1196, 1259, 1827
BlpI	GCTNAGC	1 cut(s)	1254
BlsI	GCNGC	5 cut(s)	936, 1105, 1197, 1260, 1828
BmcAI	AGTACT	1 cut(s)	1498
Bme1390I	CCNGG	4 cut(s)	1242, 1293, 1677, 1952
Bme18I	GGWCC	3 cut(s)	121, 1373, 1730
BmeT110I	CYCGRG	3 cut(s)	125, 545, 1338
BmgT120I	GGNCC	4 cut(s)	121, 1244, 1373, 1730
BmiI	GGNNCC	7 cut(s)	219, 286, 1510, 1669, 1681, 1923, 1935
BmrFI	CCNGG	4 cut(s)	1242, 1293, 1677, 1952
BmrI	ACTGGG	1 cut(s)	1488
BmsI	GCATC	6 cut(s)	496, 517, 769, 1021, 1333, 1547
BmuI	ACTGGG	1 cut(s)	1488
BoxI	GACNNNNGTC	1 cut(s)	11
BpmI	CTGGAG	1 cut(s)	320
Bpu1102I	GCTNAGC	1 cut(s)	1254
Bpu14I	TTCGAA	1 cut(s)	308
BpuEI	CTTGAG	2 cut(s)	59, 1695
BpuMI	CCSGG	1 cut(s)	1952
BsaAI	YACGTR	1 cut(s)	457
BsaHI	GRCGYC	4 cut(s)	294, 420, 603, 1934
BsaI	GGTCTC	2 cut(s)	194, 879
BsaJI	CCNNGG	3 cut(s)	1177, 1291, 1684
BsaWI	WCCGGW	3 cut(s)	26, 287, 859
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	5 cut(s)	114, 118, 281, 1053, 1271
Bse1I	ACTGG	5 cut(s)	337, 662, 1494, 1846, 1874
Bse21I	CCTNAGG	1 cut(s)	1248
Bse3DI	GCAATG	2 cut(s)	421, 699
BseBI	CCWGG	3 cut(s)	1242, 1293, 1677
BseDI	CCNNGG	3 cut(s)	1177, 1291, 1684
BseGI	GGATG	8 cut(s)	156, 511, 532, 784, 906, 1084, 1324, 1902
BseLI	CCNNNNNNNGG	5 cut(s)	114, 118, 281, 1053, 1271
BseMI	GCAATG	2 cut(s)	421, 699
BseMII	CTCAG	7 cut(s)	1161, 1213, 1259, 1268, 1767, 1781, 2003
BseNI	ACTGG	5 cut(s)	337, 662, 1494, 1846, 1874
BsePI	GCGCGC	1 cut(s)	1980
BseRI	GAGGAG	2 cut(s)	62, 143
BseXI	GCAGC	3 cut(s)	1090, 1207, 1813
BseYI	CCCAGC	2 cut(s)	530, 1999
BsgI	GTGCAG	3 cut(s)	840, 1461, 1599
Bsh1236I	CGCG	3 cut(s)	1389, 1881, 1982
Bsh1285I	CGRYCG	1 cut(s)	1652
BshFI	GGCC	4 cut(s)	46, 1085, 1245, 1689
BshNI	GGYRCC	3 cut(s)	1508, 1679, 1933
BsiEI	CGRYCG	1 cut(s)	1652
BsiHKAI	GWGCWC	2 cut(s)	135, 546
BsiHKCI	CYCGRG	3 cut(s)	125, 545, 1338
BsiSI	CCGG	6 cut(s)	27, 47, 215, 288, 860, 1951
BslFI	GGGAC	1 cut(s)	407
BslI	CCNNNNNNNGG	5 cut(s)	114, 118, 281, 1053, 1271
BsmAI	GTCTC	3 cut(s)	194, 879, 1740
BsmBI	CGTCTC	1 cut(s)	1740
BsmFI	GGGAC	1 cut(s)	407
BsmI	GAATGC	1 cut(s)	1473
BsnI	GGCC	4 cut(s)	46, 1085, 1245, 1689
Bso31I	GGTCTC	2 cut(s)	194, 879
BsoBI	CYCGRG	3 cut(s)	125, 545, 1338
Bsp119I	TTCGAA	1 cut(s)	308
Bsp1286I	GDGCHC	3 cut(s)	135, 546, 1796
Bsp143I	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
Bsp1720I	GCTNAGC	1 cut(s)	1254
BspACI	CCGC	8 cut(s)	139, 221, 327, 478, 503, 934, 1259, 1490
BspANI	GGCC	4 cut(s)	46, 1085, 1245, 1689
BspCNI	CTCAG	7 cut(s)	1162, 1212, 1260, 1267, 1766, 1782, 2002
BspFNI	CGCG	3 cut(s)	1389, 1881, 1982
BspHI	TCATGA	1 cut(s)	1546
BspLI	GGNNCC	7 cut(s)	219, 286, 1510, 1669, 1681, 1923, 1935
BspMI	ACCTGC	1 cut(s)	419
BspPI	GGATC	4 cut(s)	1168, 1861, 1916, 1929
BspT104I	TTCGAA	1 cut(s)	308
BspT107I	GGYRCC	3 cut(s)	1508, 1679, 1933
BspTI	CTTAAG	1 cut(s)	512
BspTNI	GGTCTC	2 cut(s)	194, 879
BsrBI	CCGCTC	2 cut(s)	141, 478
BsrDI	GCAATG	2 cut(s)	421, 699
BsrI	ACTGG	5 cut(s)	337, 662, 1494, 1846, 1874
BssECI	CCNNGG	3 cut(s)	1177, 1291, 1684
BssHII	GCGCGC	1 cut(s)	1980
BssMI	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
BssNI	GRCGYC	4 cut(s)	294, 420, 603, 1934
BssSI	CACGAG	1 cut(s)	1694
BssT1I	CCWWGG	1 cut(s)	1177
Bst2BI	CACGAG	1 cut(s)	1694
Bst2UI	CCWGG	3 cut(s)	1242, 1293, 1677
Bst6I	CTCTTC	1 cut(s)	192
BstACI	GRCGYC	4 cut(s)	294, 420, 603, 1934
BstAFI	CTTAAG	1 cut(s)	512
BstBAI	YACGTR	1 cut(s)	457
BstBI	TTCGAA	1 cut(s)	308
BstC8I	GCNNGC	5 cut(s)	702, 1536, 1691, 1888, 1982
BstDSI	CCRYGG	1 cut(s)	1684
BstEII	GGTNACC	1 cut(s)	29
BstF5I	GGATG	8 cut(s)	156, 511, 532, 784, 906, 1084, 1324, 1902
BstFNI	CGCG	3 cut(s)	1389, 1881, 1982
BstH2I	RGCGCY	1 cut(s)	1937
BstHHI	GCGC	6 cut(s)	511, 1391, 1881, 1936, 1982, 1984
BstKTI	GATC	6 cut(s)	847, 1176, 1652, 1789, 1856, 1924
BstMAI	GTCTC	3 cut(s)	194, 879, 1740
BstMBI	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
BstMCI	CGRYCG	1 cut(s)	1652
BstMWI	GCNNNNNNNGC	8 cut(s)	716, 893, 1306, 1349, 1419, 1616, 1887, 1990
BstNI	CCWGG	3 cut(s)	1242, 1293, 1677
BstPAI	GACNNNNGTC	1 cut(s)	11
BstPI	GGTNACC	1 cut(s)	29
BstSCI	CCNGG	4 cut(s)	1240, 1291, 1675, 1950
BstSFI	CTRYAG	1 cut(s)	1915
BstUI	CGCG	3 cut(s)	1389, 1881, 1982
BstV1I	GCAGC	3 cut(s)	1090, 1207, 1813
BstX2I	RGATCY	3 cut(s)	1173, 1786, 1921
BstXI	CCANNNNNNTGG	1 cut(s)	748
BstYI	RGATCY	3 cut(s)	1173, 1786, 1921
Bsu36I	CCTNAGG	1 cut(s)	1248
BsuRI	GGCC	4 cut(s)	46, 1085, 1245, 1689
BtgI	CCRYGG	1 cut(s)	1684
BtsCI	GGATG	8 cut(s)	156, 511, 532, 784, 906, 1084, 1324, 1902
BtsI	GCAGTG	4 cut(s)	558, 1529, 1774, 1962
BtsIMutI	CAGTG	8 cut(s)	558, 606, 669, 814, 1529, 1774, 1881, 1962
BveI	ACCTGC	1 cut(s)	419
Cac8I	GCNNGC	5 cut(s)	702, 1536, 1691, 1888, 1982
CaiI	CAGNNNCTG	2 cut(s)	775, 1241
CciI	TCATGA	1 cut(s)	1546
CfoI	GCGC	6 cut(s)	511, 1391, 1881, 1936, 1982, 1984
Cfr13I	GGNCC	4 cut(s)	121, 1244, 1373, 1730
CseI	GACGC	3 cut(s)	302, 409, 950
Csp6I	GTAC	7 cut(s)	454, 992, 1284, 1385, 1497, 1597, 1680
CviAII	CATG	5 cut(s)	40, 959, 1547, 1739, 1775
CviQI	GTAC	7 cut(s)	454, 992, 1284, 1385, 1497, 1597, 1680
DinI	GGCGCC	1 cut(s)	1935
DpnI	GATC	6 cut(s)	846, 1175, 1651, 1788, 1855, 1923
DpnII	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
DraI	TTTAAA	3 cut(s)	256, 346, 1014
DrdI	GACNNNNNNGTC	1 cut(s)	1732
DseDI	GACNNNNNNGTC	1 cut(s)	1732
EaeI	YGGCCR	1 cut(s)	1687
Eam1104I	CTCTTC	1 cut(s)	192
EarI	CTCTTC	1 cut(s)	192
EciI	GGCGGA	1 cut(s)	1479
Eco130I	CCWWGG	1 cut(s)	1177
Eco24I	GRGCYC	1 cut(s)	1796
Eco31I	GGTCTC	2 cut(s)	194, 879
Eco32I	GATATC	1 cut(s)	679
Eco47I	GGWCC	3 cut(s)	121, 1373, 1730
Eco57I	CTGAAG	1 cut(s)	1767
Eco81I	CCTNAGG	1 cut(s)	1248
Eco88I	CYCGRG	3 cut(s)	125, 545, 1338
Eco91I	GGTNACC	1 cut(s)	29
EcoO109I	RGGNCCY	1 cut(s)	121
EcoO65I	GGTNACC	1 cut(s)	29
EcoRI	GAATTC	1 cut(s)	850
EcoRII	CCWGG	3 cut(s)	1240, 1291, 1675
EcoRV	GATATC	1 cut(s)	679
EcoT14I	CCWWGG	1 cut(s)	1177
EcoT22I	ATGCAT	1 cut(s)	964
EcoT38I	GRGCYC	1 cut(s)	1796
EgeI	GGCGCC	1 cut(s)	1935
EheI	GGCGCC	1 cut(s)	1935
ErhI	CCWWGG	1 cut(s)	1177
Esp3I	CGTCTC	1 cut(s)	1740
FaeI	CATG	5 cut(s)	43, 962, 1550, 1742, 1778
FalI	AAGNNNNNCTT	2 cut(s)	1050, 1082
FaqI	GGGAC	1 cut(s)	407
FatI	CATG	5 cut(s)	39, 958, 1546, 1738, 1774
FauI	CCCGC	1 cut(s)	471
FblI	GTMKAC	3 cut(s)	5, 15, 407
Fnu4HI	GCNGC	5 cut(s)	935, 1104, 1196, 1259, 1827
FokI	GGATG	8 cut(s)	143, 518, 539, 791, 893, 1091, 1311, 1889
FriOI	GRGCYC	1 cut(s)	1796
Fsp4HI	GCNGC	5 cut(s)	935, 1104, 1196, 1259, 1827
FspBI	CTAG	2 cut(s)	539, 615
GlaI	GCGC	6 cut(s)	510, 1390, 1880, 1935, 1981, 1983
GluI	GCNGC	5 cut(s)	935, 1104, 1196, 1259, 1827
GsaI	CCCAGC	2 cut(s)	534, 2003
GsuI	CTGGAG	1 cut(s)	320
HaeII	RGCGCY	1 cut(s)	1937
HaeIII	GGCC	4 cut(s)	46, 1085, 1245, 1689
HapII	CCGG	6 cut(s)	27, 47, 215, 288, 860, 1951
HgaI	GACGC	3 cut(s)	302, 409, 950
HhaI	GCGC	6 cut(s)	511, 1391, 1881, 1936, 1982, 1984
Hin1I	GRCGYC	4 cut(s)	294, 420, 603, 1934
Hin1II	CATG	5 cut(s)	43, 962, 1550, 1742, 1778
Hin6I	GCGC	6 cut(s)	509, 1389, 1879, 1934, 1980, 1982
HinP1I	GCGC	6 cut(s)	509, 1389, 1879, 1934, 1980, 1982
HincII	GTYRAC	3 cut(s)	6, 16, 408
HindII	GTYRAC	3 cut(s)	6, 16, 408
HindIII	AAGCTT	5 cut(s)	698, 824, 914, 1142, 1420
HinfI	GANTC	5 cut(s)	314, 359, 575, 951, 1604
HpaII	CCGG	6 cut(s)	27, 47, 215, 288, 860, 1951
HphI	GGTGA	7 cut(s)	41, 302, 316, 661, 736, 809, 2003
Hpy166II	GTNNAC	4 cut(s)	6, 16, 408, 1699
Hpy188III	TCNNGA	9 cut(s)	76, 424, 776, 1024, 1169, 1547, 1622, 1696, 1712
Hpy8I	GTNNAC	4 cut(s)	6, 16, 408, 1699
Hpy99I	CGWCG	4 cut(s)	17, 368, 389, 1462
HpyAV	CCTTC	6 cut(s)	44, 73, 709, 1197, 1599, 1830
HpyCH4IV	ACGT	4 cut(s)	456, 603, 1123, 1282
HpyF10VI	GCNNNNNNNGC	8 cut(s)	716, 893, 1306, 1349, 1419, 1616, 1887, 1990
HpySE526I	ACGT	4 cut(s)	456, 603, 1123, 1282
Hsp92I	GRCGYC	4 cut(s)	294, 420, 603, 1934
Hsp92II	CATG	5 cut(s)	43, 962, 1550, 1742, 1778
HspAI	GCGC	6 cut(s)	509, 1389, 1879, 1934, 1980, 1982
KasI	GGCGCC	1 cut(s)	1933
KpnI	GGTACC	1 cut(s)	1683
Kzo9I	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
LmnI	GCTCC	8 cut(s)	49, 130, 146, 217, 223, 595, 1243, 1624
Lsp1109I	GCAGC	3 cut(s)	1090, 1207, 1813
LweI	GCATC	6 cut(s)	496, 517, 769, 1021, 1333, 1547
MaeI	CTAG	2 cut(s)	539, 615
MaeII	ACGT	4 cut(s)	456, 603, 1123, 1282
MaeIII	GTNAC	8 cut(s)	29, 290, 322, 491, 604, 610, 636, 1777
MalI	GATC	6 cut(s)	846, 1175, 1651, 1788, 1855, 1923
MbiI	CCGCTC	2 cut(s)	141, 478
MboI	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
MboII	GAAGA	2 cut(s)	209, 281
MfeI	CAATTG	2 cut(s)	278, 299
MflI	RGATCY	3 cut(s)	1173, 1786, 1921
MhlI	GDGCHC	3 cut(s)	135, 546, 1796
MlsI	TGGCCA	1 cut(s)	1689
MluCI	AATT	9 cut(s)	21, 151, 245, 250, 262, 278, 299, 353, 850
MluNI	TGGCCA	1 cut(s)	1689
Mly113I	GGCGCC	1 cut(s)	1934
MmeI	TCCRAC	1 cut(s)	765
Mox20I	TGGCCA	1 cut(s)	1689
Mph1103I	ATGCAT	1 cut(s)	964
MscI	TGGCCA	1 cut(s)	1689
MseI	TTAA	9 cut(s)	255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
Msp20I	TGGCCA	1 cut(s)	1689
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	6 cut(s)	27, 47, 215, 288, 860, 1951
MspR9I	CCNGG	4 cut(s)	1242, 1293, 1677, 1952
MunI	CAATTG	2 cut(s)	278, 299
Mva1269I	GAATGC	1 cut(s)	1473
MvaI	CCWGG	3 cut(s)	1242, 1293, 1677
MvnI	CGCG	3 cut(s)	1389, 1881, 1982
MwoI	GCNNNNNNNGC	8 cut(s)	716, 893, 1306, 1349, 1419, 1616, 1887, 1990
NarI	GGCGCC	1 cut(s)	1934
NciI	CCSGG	1 cut(s)	1952
NdeII	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
NlaIII	CATG	5 cut(s)	43, 962, 1550, 1742, 1778
NlaIV	GGNNCC	7 cut(s)	219, 286, 1510, 1669, 1681, 1923, 1935
NmuCI	GTSAC	5 cut(s)	29, 290, 322, 491, 604
NsiI	ATGCAT	1 cut(s)	964
NspV	TTCGAA	1 cut(s)	308
PaeR7I	CTCGAG	3 cut(s)	125, 545, 1338
PagI	TCATGA	1 cut(s)	1546
PalAI	GGCGCGCC	1 cut(s)	1980
PauI	GCGCGC	1 cut(s)	1980
PcsI	WCGNNNNNNNCGW	2 cut(s)	363, 1336
PctI	GAATGC	1 cut(s)	1473
PfeI	GAWTC	5 cut(s)	314, 359, 575, 951, 1604
PkrI	GCNGC	5 cut(s)	936, 1105, 1197, 1260, 1828
Ple19I	CGATCG	1 cut(s)	1652
PluTI	GGCGCC	1 cut(s)	1937
Ppu21I	YACGTR	1 cut(s)	457
PpuMI	RGGWCCY	1 cut(s)	121
PshAI	GACNNNNGTC	1 cut(s)	11
Psp5II	RGGWCCY	1 cut(s)	121
Psp6I	CCWGG	3 cut(s)	1240, 1291, 1675
PspEI	GGTNACC	1 cut(s)	29
PspFI	CCCAGC	2 cut(s)	530, 1999
PspGI	CCWGG	3 cut(s)	1240, 1291, 1675
PspN4I	GGNNCC	7 cut(s)	219, 286, 1510, 1669, 1681, 1923, 1935
PspPI	GGNCC	4 cut(s)	121, 1244, 1373, 1730
PspPPI	RGGWCCY	1 cut(s)	121
PspXI	VCTCGAGB	3 cut(s)	125, 545, 1338
PstNI	CAGNNNCTG	2 cut(s)	775, 1241
PsuI	RGATCY	3 cut(s)	1173, 1786, 1921
PteI	GCGCGC	1 cut(s)	1980
PvuI	CGATCG	1 cut(s)	1652
RsaI	GTAC	7 cut(s)	455, 993, 1285, 1386, 1498, 1598, 1681
RsaNI	GTAC	7 cut(s)	454, 992, 1284, 1385, 1497, 1597, 1680
SalI	GTCGAC	3 cut(s)	4, 14, 406
SaqAI	TTAA	9 cut(s)	255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
SatI	GCNGC	5 cut(s)	935, 1104, 1196, 1259, 1827
Sau3AI	GATC	6 cut(s)	844, 1173, 1649, 1786, 1853, 1921
Sau96I	GGNCC	4 cut(s)	121, 1244, 1373, 1730
ScaI	AGTACT	1 cut(s)	1498
ScrFI	CCNGG	4 cut(s)	1242, 1293, 1677, 1952
SduI	GDGCHC	3 cut(s)	135, 546, 1796
SfaNI	GCATC	6 cut(s)	496, 517, 769, 1021, 1333, 1547
SfcI	CTRYAG	1 cut(s)	1915
SfoI	GGCGCC	1 cut(s)	1935
Sfr274I	CTCGAG	3 cut(s)	125, 545, 1338
SfuI	TTCGAA	1 cut(s)	308
SgsI	GGCGCGCC	1 cut(s)	1980
SinI	GGWCC	3 cut(s)	121, 1373, 1730
SlaI	CTCGAG	3 cut(s)	125, 545, 1338
SmlI	CTYRAG	6 cut(s)	74, 125, 512, 545, 1338, 1710
SmoI	CTYRAG	6 cut(s)	74, 125, 512, 545, 1338, 1710
Sse9I	AATT	9 cut(s)	21, 151, 245, 250, 262, 278, 299, 353, 850
SsiI	CCGC	8 cut(s)	139, 221, 327, 478, 503, 934, 1259, 1490
SspDI	GGCGCC	1 cut(s)	1933
SspI	AATATT	1 cut(s)	1163
SspMI	CTAG	2 cut(s)	539, 615
StyD4I	CCNGG	4 cut(s)	1240, 1291, 1675, 1950
StyI	CCWWGG	1 cut(s)	1177
TaiI	ACGT	4 cut(s)	459, 606, 1126, 1285
TasI	AATT	9 cut(s)	21, 151, 245, 250, 262, 278, 299, 353, 850
TatI	WGTACW	1 cut(s)	1496
TauI	GCSGC	2 cut(s)	937, 1261
TfiI	GAWTC	5 cut(s)	314, 359, 575, 951, 1604
Tru1I	TTAA	9 cut(s)	255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
Tru9I	TTAA	9 cut(s)	255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
TscAI	CASTG	8 cut(s)	558, 613, 669, 814, 1536, 1774, 1881, 1962
TseFI	GTSAC	5 cut(s)	29, 290, 322, 491, 604
TseI	GCWGC	3 cut(s)	1103, 1195, 1826
Tsp45I	GTSAC	5 cut(s)	29, 290, 322, 491, 604
TspDTI	ATGAA	5 cut(s)	710, 895, 1009, 1555, 1733
TspGWI	ACGGA	2 cut(s)	352, 1722
TspRI	CASTG	8 cut(s)	558, 613, 669, 814, 1536, 1774, 1881, 1962
Vha464I	CTTAAG	1 cut(s)	512
VpaK11BI	GGWCC	3 cut(s)	121, 1373, 1730
XapI	RAATTY	3 cut(s)	21, 250, 850
XhoI	CTCGAG	3 cut(s)	125, 545, 1338
XmiI	GTMKAC	3 cut(s)	5, 15, 407
XspI	CTAG	2 cut(s)	539, 615
ZraI	GACGTC	1 cut(s)	604
ZrmI	AGTACT	1 cut(s)	1498
Zsp2I	ATGCAT	1 cut(s)	964

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.