Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0012633

Physical Location & Seq

Genomic Coordinates Forward (+)

30656848 .. 30660063

Gene Structure 3216 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1231700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGTCGAGCACCGTCGACAAAATCTCCGGCGACCTTGACATGACGCCGGAGCTACGCAAATCCTTCAACCTCCTCAAGACCCACGCCTCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACCTCGAGGAGCACTTCCGCTGCATCGACAATTCCATCCAATCCAAGCTCGACCAACTCCAAAAACAACAATCGGAAGAGGCCCATCTCACTCCGGCTCCGCAATCGCAACCCAAAGAACCCGAATTGAATTTTAAACCCAATTCCGAAGAACCCCAATTGGGTTCCGGTGACGCCCAATTGAGTTCGAAAGAATCCCAAGTCACCGCCACCCTCCAATCCTTGAAAACCCAATTCGATTCCGTTGAAACCCAAGTCAAAACCGACGAGCCTTCGCCGGAAATGTCGACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTATGCGAATGAGCATTTGAAAGAGAAGGGTTCGGTGCGTGATGGTATTGTGAATGCGCTTAAGGCTTCTGTGGATGCTGGGAAGCTAGTGCTCGAGGCAGTGAAATGGTTTTATCCGTCAGAATCGAATAAGGGAGGTAAGGAGCTTGACTTCAGTGTTACCAGGAAAAGTTGTGCGATTTTGTTACAAGGGCTGGTGAATGTGAAACCAGTGATTAAACCCGAGATCATCAGAGAGGCAATGAAGCTTGCATTGGCTTGGAAGGCAAAGGTGATAACTCAAATGTCGAACTCTTTGGAGGTTTGGGTTTTTTTTCAGCTTCTGGATGCTTTTGGGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGAAGCTTTTTAGTTGCGTAGCAGATCGGTATTCTGCACCGGAGTTGTTTAGGTCCCTTGGTTTCGCCGATAAAGCCTCTGATTTCATCCAAAAGCTTATATCAGAGAATAAGCGGGTTGATGCTGTTAGATTCAGTCGTGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCATCACGGAAGATGCAAGGACAAGTTCCAAGAGTGGGCAAGACCATCTTAAAGCACAGGATGAGTCCTTAGACAAAGAAATAGCTGCTCTAATAGGCATAAAACGTTGCGTTGAGGACTACAAGCTTGTTGTTGAACCAGAATATTATCCCAAGACCCTCGGAAAACACATTAGGCAGCTGAAAAGGGTGAGAAAACATAGGAAAGCAACTCTAAGGGAGAGTAAACATAGGAAAGCAACTCTAAGAGCCCCTGGCCCTAAGGCTCAGCCGCAACCCCTGAGTGGAAAGAAACGTACTGCCCCAGGTCAGCCAAATGAGCAGAACAGAAATAAGCATCCTCGAACAGCTCCAGCTTTAGCTGCTCAAACTGTTTCTCTCAGAGGACCAGCCACAGTAGGCGCTATACAACCATCTTATATTCGCCAAGTAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGTTATCGTCAGCTGGAATGCCCAGACAAGCAACCACTCCGCCCAGTACTTCTTATGGCGCCATTTCCACCTTGAACCCACTGCGAGCATCTCATCATCAGCAAGGAGGCTCATTCATCGGTCAAGGTGCAGAATACCCTTCTCGATACTATGATTCAGCACACTACGCTCCTGATACTATTAATAACTTATCTGCTCGATCGTATGATTTGGTGGGTTCCTCTCCTGGTACCCGTGGCCAGCTCGTGAACTTAACAGCTCGAGACTATGGATTGACGGGTCCGTCTCATGTACATACTAACTCAGAACCTGGGGGCAGTGGTCCAGATCTGAGTCCATTAGTCGCACGATACAGAACAAGTATACCTGCAAATGGAAGGACTGGACAGTTAGGATCAGCAGGAAGCCCTACACCGATGCGCATTGCAAGCCCGAACAGTTCCATCCCCTATGCCTATAGGGATCCGCTTAGGGCGCCTAACTACAATGACCGGGCGGTGCCTTCCAGCAGTGGCTATGGCGCACCGTCTCAGCACCCCCCAGCCATTTACCACCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

680

Amino Acids

74.51

Weight (kDa)

9.3

Isoelectric Point (pI)

48.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Frigida	PF07899	145 - 409	1.7e-65	Frigida-like protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04240 FvH4_6g04240
malus_domestica	MD00G1051400.v1.1 MD12G1231700.v1.1 MD12G1232000.v1.1
prunus_persica	Prupe.6G334900_v2.0.a1
pyrus_communis	pycom04g19180 pycom12g21440
rosa_chinensis	RchiOBHm_Chr3g0452911
rosa_laevigata	RLG00000025560
rosa_multiflora	Rmu_sc0002823.1_g000005 Rmu_sc0002823.1_g000006
rosa_roxburghii	Rroxscaffold_6G00429920
rosa_rugosa	Rorug02G0643600
rosa_samantha	Rh3AG046400 Rh3BG048900 Rh3CG047500 Rh3DG048500
rosa_wichuraiana	Rw3G003650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1762
AbsI	CCTCGAGG	1 cut(s)	125
Acc16I	TGCGCA	1 cut(s)	1901
Acc36I	ACCTGC	2 cut(s)	419, 1855
Acc65I	GGTACC	1 cut(s)	1709
AccB1I	GGYRCC	4 cut(s)	1538, 1709, 1954, 1978
AccI	GTMKAC	3 cut(s)	15, 407, 1843
AciI	CCGC	8 cut(s)	139, 221, 327, 934, 1289, 1520, 1946, 1976
AclI	AACGTT	1 cut(s)	1123
AclWI	GGATC	3 cut(s)	1882, 1937, 1950
AcoI	YGGCCR	1 cut(s)	1717
AcsI	RAATTY	1 cut(s)	250
AcuI	CTGAAG	1 cut(s)	589
AcyI	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
AfaI	GTAC	5 cut(s)	993, 1315, 1528, 1711, 1773
AfiI	CCNNNNNNNGG	6 cut(s)	114, 118, 281, 1053, 1301, 1853
AflII	CTTAAG	1 cut(s)	512
AgsI	TTSAA	8 cut(s)	67, 250, 346, 368, 472, 823, 1154, 1555
AjnI	CCWGG	5 cut(s)	614, 1270, 1321, 1705, 1789
AjuI	GAANNNNNNNTTGG	2 cut(s)	689, 721
Alw21I	GWGCWC	3 cut(s)	11, 135, 546
Alw26I	GTCTC	3 cut(s)	1737, 1770, 2013
AlwI	GGATC	3 cut(s)	1882, 1937, 1950
AlwNI	CAGNNNCTG	2 cut(s)	775, 1790
Ama87I	CYCGRG	4 cut(s)	125, 545, 674, 1740
AoxI	GGCC	3 cut(s)	201, 1273, 1717
ApeKI	GCWGC	4 cut(s)	141, 1103, 1195, 1379
ApoI	RAATTY	1 cut(s)	250
AseI	ATTAAT	1 cut(s)	1662
Asp718I	GGTACC	1 cut(s)	1709
AspLEI	GCGC	6 cut(s)	511, 1421, 1541, 1902, 1957, 2003
AspS9I	GGNCC	7 cut(s)	121, 202, 873, 1274, 1403, 1760, 1802
AsuC2I	CCSGG	1 cut(s)	1973
AsuHPI	GGTGA	6 cut(s)	302, 316, 661, 736, 809, 1219
AsuII	TTCGAA	1 cut(s)	308
AvaI	CYCGRG	4 cut(s)	125, 545, 674, 1740
AvaII	GGWCC	5 cut(s)	121, 873, 1403, 1760, 1802
AxyI	CCTNAGG	1 cut(s)	1278
BalI	TGGCCA	1 cut(s)	1719
BamHI	GGATCC	1 cut(s)	1942
BanI	GGYRCC	4 cut(s)	1538, 1709, 1954, 1978
BanII	GRGCYC	1 cut(s)	1270
BauI	CACGAG	1 cut(s)	1724
Bbv12I	GWGCWC	3 cut(s)	11, 135, 546
BbvI	GCAGC	4 cut(s)	128, 1090, 1207, 1366
BccI	CCATC	6 cut(s)	164, 213, 488, 1071, 1438, 1931
BceAI	ACGGC	1 cut(s)	451
BciT130I	CCWGG	5 cut(s)	616, 1272, 1323, 1707, 1791
BcnI	CCSGG	1 cut(s)	1973
BcoDI	GTCTC	3 cut(s)	1737, 1770, 2013
BfaI	CTAG	1 cut(s)	539
BfmI	CTRYAG	1 cut(s)	1936
BfoI	RGCGCY	3 cut(s)	1422, 1542, 1958
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	2 cut(s)	419, 1855
BglII	AGATCT	1 cut(s)	1807
BisI	GCNGC	5 cut(s)	142, 1104, 1196, 1289, 1380
BlpI	GCTNAGC	1 cut(s)	1284
BlsI	GCNGC	5 cut(s)	143, 1105, 1197, 1290, 1381
BmcAI	AGTACT	1 cut(s)	1528
Bme1390I	CCNGG	6 cut(s)	616, 1272, 1323, 1707, 1791, 1973
Bme18I	GGWCC	5 cut(s)	121, 873, 1403, 1760, 1802
BmeT110I	CYCGRG	4 cut(s)	125, 545, 674, 1740
BmgT120I	GGNCC	7 cut(s)	121, 202, 873, 1274, 1403, 1760, 1802
BmrFI	CCNGG	6 cut(s)	616, 1272, 1323, 1707, 1791, 1973
BmrI	ACTGGG	1 cut(s)	1518
BmsI	GCATC	8 cut(s)	153, 517, 769, 931, 1021, 1363, 1577, 1887
BmuI	ACTGGG	1 cut(s)	1518
BpmI	CTGGAG	1 cut(s)	1353
Bpu10I	CCTNAGC	1 cut(s)	1949
Bpu1102I	GCTNAGC	1 cut(s)	1284
Bpu14I	TTCGAA	1 cut(s)	308
BpuEI	CTTGAG	1 cut(s)	59
BpuMI	CCSGG	1 cut(s)	1973
BsaHI	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
BsaJI	CCNNGG	6 cut(s)	877, 1177, 1270, 1321, 1714, 1790
BsaWI	WCCGGW	2 cut(s)	287, 859
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 202, 232
Bsc4I	CCNNNNNNNGG	6 cut(s)	114, 118, 281, 1053, 1301, 1853
Bse1I	ACTGG	3 cut(s)	662, 1524, 1867
Bse21I	CCTNAGG	1 cut(s)	1278
Bse3DI	GCAATG	3 cut(s)	421, 699, 1902
BseBI	CCWGG	5 cut(s)	616, 1272, 1323, 1707, 1791
BseDI	CCNNGG	6 cut(s)	877, 1177, 1270, 1321, 1714, 1790
BseGI	GGATG	7 cut(s)	156, 532, 784, 906, 1084, 1354, 1923
BseLI	CCNNNNNNNGG	6 cut(s)	114, 118, 281, 1053, 1301, 1853
BseMI	GCAATG	3 cut(s)	421, 699, 1902
BseMII	CTCAG	6 cut(s)	1289, 1298, 1411, 1797, 1802, 2024
BseNI	ACTGG	3 cut(s)	662, 1524, 1867
BseRI	GAGGAG	2 cut(s)	62, 143
BseXI	GCAGC	4 cut(s)	128, 1090, 1207, 1366
BseYI	CCCAGC	2 cut(s)	530, 2020
BsgI	GTGCAG	3 cut(s)	840, 1491, 1629
Bsh1285I	CGRYCG	1 cut(s)	1682
BshFI	GGCC	3 cut(s)	203, 1275, 1719
BshNI	GGYRCC	4 cut(s)	1538, 1709, 1954, 1978
BsiEI	CGRYCG	1 cut(s)	1682
BsiHKAI	GWGCWC	3 cut(s)	11, 135, 546
BsiHKCI	CYCGRG	4 cut(s)	125, 545, 674, 1740
BsiSI	CCGG	7 cut(s)	27, 47, 215, 288, 398, 860, 1972
BslFI	GGGAC	2 cut(s)	407, 859
BslI	CCNNNNNNNGG	6 cut(s)	114, 118, 281, 1053, 1301, 1853
BsmAI	GTCTC	3 cut(s)	1737, 1770, 2013
BsmBI	CGTCTC	2 cut(s)	1770, 2013
BsmFI	GGGAC	2 cut(s)	407, 859
BsmI	GAATGC	2 cut(s)	511, 1503
BsnI	GGCC	3 cut(s)	203, 1275, 1719
BsoBI	CYCGRG	4 cut(s)	125, 545, 674, 1740
Bsp119I	TTCGAA	1 cut(s)	308
Bsp1286I	GDGCHC	4 cut(s)	11, 135, 546, 1270
Bsp1407I	TGTACA	1 cut(s)	1771
Bsp143I	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
Bsp1720I	GCTNAGC	1 cut(s)	1284
BspACI	CCGC	8 cut(s)	139, 221, 327, 934, 1289, 1520, 1946, 1976
BspANI	GGCC	3 cut(s)	203, 1275, 1719
BspCNI	CTCAG	6 cut(s)	1290, 1297, 1410, 1796, 1803, 2023
BspMI	ACCTGC	2 cut(s)	419, 1855
BspPI	GGATC	3 cut(s)	1882, 1937, 1950
BspT104I	TTCGAA	1 cut(s)	308
BspT107I	GGYRCC	4 cut(s)	1538, 1709, 1954, 1978
BspTI	CTTAAG	1 cut(s)	512
BsrDI	GCAATG	3 cut(s)	421, 699, 1902
BsrGI	TGTACA	1 cut(s)	1771
BsrI	ACTGG	3 cut(s)	662, 1524, 1867
BssECI	CCNNGG	6 cut(s)	877, 1177, 1270, 1321, 1714, 1790
BssMI	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
BssNAI	GTATAC	1 cut(s)	1844
BssNI	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
BssSI	CACGAG	1 cut(s)	1724
BssT1I	CCWWGG	1 cut(s)	877
Bst1107I	GTATAC	1 cut(s)	1844
Bst2BI	CACGAG	1 cut(s)	1724
Bst2UI	CCWGG	5 cut(s)	616, 1272, 1323, 1707, 1791
Bst4CI	ACNGT	9 cut(s)	13, 809, 972, 1390, 1414, 1463, 1869, 1919, 2007
Bst6I	CTCTTC	1 cut(s)	192
BstACI	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
BstAFI	CTTAAG	1 cut(s)	512
BstAUI	TGTACA	1 cut(s)	1771
BstBI	TTCGAA	1 cut(s)	308
BstC8I	GCNNGC	4 cut(s)	702, 1566, 1721, 1909
BstDSI	CCRYGG	1 cut(s)	1714
BstF5I	GGATG	7 cut(s)	156, 532, 784, 906, 1084, 1354, 1923
BstH2I	RGCGCY	3 cut(s)	1422, 1542, 1958
BstHHI	GCGC	6 cut(s)	511, 1421, 1541, 1902, 1957, 2003
BstKTI	GATC	6 cut(s)	681, 847, 1682, 1810, 1877, 1945
BstMAI	GTCTC	3 cut(s)	1737, 1770, 2013
BstMBI	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
BstMCI	CGRYCG	1 cut(s)	1682
BstMWI	GCNNNNNNNGC	9 cut(s)	515, 716, 893, 1336, 1379, 1449, 1646, 1908, 1954
BstNI	CCWGG	5 cut(s)	616, 1272, 1323, 1707, 1791
BstSCI	CCNGG	6 cut(s)	614, 1270, 1321, 1705, 1789, 1971
BstSFI	CTRYAG	1 cut(s)	1936
BstV1I	GCAGC	4 cut(s)	128, 1090, 1207, 1366
BstX2I	RGATCY	2 cut(s)	1807, 1942
BstYI	RGATCY	2 cut(s)	1807, 1942
BstZ17I	GTATAC	1 cut(s)	1844
Bsu36I	CCTNAGG	1 cut(s)	1278
BsuRI	GGCC	3 cut(s)	203, 1275, 1719
BtgI	CCRYGG	1 cut(s)	1714
BtsCI	GGATG	7 cut(s)	156, 532, 784, 906, 1084, 1354, 1923
BtsI	GCAGTG	4 cut(s)	558, 1559, 1804, 1996
BtsIMutI	CAGTG	7 cut(s)	558, 613, 669, 814, 1559, 1804, 1996
BveI	ACCTGC	2 cut(s)	419, 1855
Cac8I	GCNNGC	4 cut(s)	702, 1566, 1721, 1909
CaiI	CAGNNNCTG	2 cut(s)	775, 1790
CfoI	GCGC	6 cut(s)	511, 1421, 1541, 1902, 1957, 2003
Cfr13I	GGNCC	7 cut(s)	121, 202, 873, 1274, 1403, 1760, 1802
CseI	GACGC	3 cut(s)	52, 302, 409
Csp6I	GTAC	5 cut(s)	992, 1314, 1527, 1710, 1772
CviAII	CATG	2 cut(s)	40, 1769
CviQI	GTAC	5 cut(s)	992, 1314, 1527, 1710, 1772
DinI	GGCGCC	2 cut(s)	1540, 1956
DpnI	GATC	6 cut(s)	680, 846, 1681, 1809, 1876, 1944
DpnII	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
DraI	TTTAAA	2 cut(s)	256, 1014
DrdI	GACNNNNNNGTC	1 cut(s)	1762
DseDI	GACNNNNNNGTC	1 cut(s)	1762
EaeI	YGGCCR	1 cut(s)	1717
Eam1104I	CTCTTC	1 cut(s)	192
EarI	CTCTTC	1 cut(s)	192
EciI	GGCGGA	1 cut(s)	1509
Eco130I	CCWWGG	1 cut(s)	877
Eco24I	GRGCYC	1 cut(s)	1270
Eco47I	GGWCC	5 cut(s)	121, 873, 1403, 1760, 1802
Eco57I	CTGAAG	1 cut(s)	589
Eco81I	CCTNAGG	1 cut(s)	1278
Eco88I	CYCGRG	4 cut(s)	125, 545, 674, 1740
EcoO109I	RGGNCCY	2 cut(s)	121, 873
EcoRII	CCWGG	5 cut(s)	614, 1270, 1321, 1705, 1789
EcoT14I	CCWWGG	1 cut(s)	877
EcoT38I	GRGCYC	1 cut(s)	1270
EgeI	GGCGCC	2 cut(s)	1540, 1956
EheI	GGCGCC	2 cut(s)	1540, 1956
ErhI	CCWWGG	1 cut(s)	877
Esp3I	CGTCTC	2 cut(s)	1770, 2013
FaeI	CATG	2 cut(s)	43, 1772
FalI	AAGNNNNNCTT	2 cut(s)	1050, 1082
FaqI	GGGAC	2 cut(s)	407, 859
FatI	CATG	2 cut(s)	39, 1768
FauI	CCCGC	1 cut(s)	927
FblI	GTMKAC	3 cut(s)	15, 407, 1843
Fnu4HI	GCNGC	5 cut(s)	142, 1104, 1196, 1289, 1380
FokI	GGATG	7 cut(s)	143, 539, 791, 893, 1091, 1341, 1910
FriOI	GRGCYC	1 cut(s)	1270
Fsp4HI	GCNGC	5 cut(s)	142, 1104, 1196, 1289, 1380
FspAI	RTGCGCAY	1 cut(s)	1901
FspBI	CTAG	1 cut(s)	539
FspI	TGCGCA	1 cut(s)	1901
GlaI	GCGC	6 cut(s)	510, 1420, 1540, 1901, 1956, 2002
GluI	GCNGC	5 cut(s)	142, 1104, 1196, 1289, 1380
GsaI	CCCAGC	2 cut(s)	534, 2024
GsuI	CTGGAG	1 cut(s)	1353
HaeII	RGCGCY	3 cut(s)	1422, 1542, 1958
HaeIII	GGCC	3 cut(s)	203, 1275, 1719
HapII	CCGG	7 cut(s)	27, 47, 215, 288, 398, 860, 1972
HgaI	GACGC	3 cut(s)	52, 302, 409
HhaI	GCGC	6 cut(s)	511, 1421, 1541, 1902, 1957, 2003
Hin1I	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
Hin1II	CATG	2 cut(s)	43, 1772
Hin6I	GCGC	6 cut(s)	509, 1419, 1539, 1900, 1955, 2001
HinP1I	GCGC	6 cut(s)	509, 1419, 1539, 1900, 1955, 2001
HincII	GTYRAC	2 cut(s)	16, 408
HindII	GTYRAC	2 cut(s)	16, 408
HindIII	AAGCTT	5 cut(s)	698, 824, 914, 1142, 1450
HinfI	GANTC	7 cut(s)	314, 359, 575, 951, 1082, 1634, 1813
HpaII	CCGG	7 cut(s)	27, 47, 215, 288, 398, 860, 1972
HphI	GGTGA	6 cut(s)	302, 316, 661, 736, 809, 1219
Hpy166II	GTNNAC	5 cut(s)	16, 408, 1244, 1729, 1844
Hpy188III	TCNNGA	8 cut(s)	76, 424, 776, 1623, 1652, 1726, 1742, 1805
Hpy8I	GTNNAC	5 cut(s)	16, 408, 1244, 1729, 1844
Hpy99I	CGWCG	2 cut(s)	17, 389
HpyAV	CCTTC	7 cut(s)	73, 402, 472, 709, 1629, 1851, 1992
HpyCH4III	ACNGT	9 cut(s)	13, 809, 972, 1390, 1414, 1463, 1869, 1919, 2007
HpyCH4IV	ACGT	2 cut(s)	1123, 1312
HpyF10VI	GCNNNNNNNGC	9 cut(s)	515, 716, 893, 1336, 1379, 1449, 1646, 1908, 1954
HpySE526I	ACGT	2 cut(s)	1123, 1312
Hsp92I	GRCGYC	5 cut(s)	44, 294, 420, 1539, 1955
Hsp92II	CATG	2 cut(s)	43, 1772
HspAI	GCGC	6 cut(s)	509, 1419, 1539, 1900, 1955, 2001
KasI	GGCGCC	2 cut(s)	1538, 1954
KpnI	GGTACC	1 cut(s)	1713
Kzo9I	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
LmnI	GCTCC	6 cut(s)	49, 130, 223, 595, 1372, 1654
Lsp1109I	GCAGC	4 cut(s)	128, 1090, 1207, 1366
LweI	GCATC	8 cut(s)	153, 517, 769, 931, 1021, 1363, 1577, 1887
MaeI	CTAG	1 cut(s)	539
MaeII	ACGT	2 cut(s)	1123, 1312
MaeIII	GTNAC	4 cut(s)	290, 322, 610, 636
MalI	GATC	6 cut(s)	680, 846, 1681, 1809, 1876, 1944
MboI	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
MboII	GAAGA	3 cut(s)	209, 281, 1040
MfeI	CAATTG	2 cut(s)	278, 299
MflI	RGATCY	2 cut(s)	1807, 1942
MhlI	GDGCHC	4 cut(s)	11, 135, 546, 1270
MlsI	TGGCCA	1 cut(s)	1719
MluCI	AATT	7 cut(s)	151, 245, 250, 262, 278, 299, 353
MluNI	TGGCCA	1 cut(s)	1719
Mly113I	GGCGCC	2 cut(s)	1539, 1955
MlyI	GAGTC	2 cut(s)	1091, 1822
Mox20I	TGGCCA	1 cut(s)	1719
MscI	TGGCCA	1 cut(s)	1719
MseI	TTAA	8 cut(s)	255, 513, 669, 1002, 1013, 1068, 1662, 1733
Msp20I	TGGCCA	1 cut(s)	1719
MspA1I	CMGCKG	3 cut(s)	141, 1198, 1493
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	7 cut(s)	27, 47, 215, 288, 398, 860, 1972
MspR9I	CCNGG	6 cut(s)	616, 1272, 1323, 1707, 1791, 1973
MunI	CAATTG	2 cut(s)	278, 299
Mva1269I	GAATGC	2 cut(s)	511, 1503
MvaI	CCWGG	5 cut(s)	616, 1272, 1323, 1707, 1791
MwoI	GCNNNNNNNGC	9 cut(s)	515, 716, 893, 1336, 1379, 1449, 1646, 1908, 1954
NarI	GGCGCC	2 cut(s)	1539, 1955
NciI	CCSGG	1 cut(s)	1973
NdeII	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
NlaIII	CATG	2 cut(s)	43, 1772
NmuCI	GTSAC	2 cut(s)	290, 322
NsbI	TGCGCA	1 cut(s)	1901
NspV	TTCGAA	1 cut(s)	308
PaeR7I	CTCGAG	3 cut(s)	125, 545, 1740
PctI	GAATGC	2 cut(s)	511, 1503
PfeI	GAWTC	5 cut(s)	314, 359, 575, 951, 1634
PkrI	GCNGC	5 cut(s)	143, 1105, 1197, 1290, 1381
Ple19I	CGATCG	1 cut(s)	1682
PleI	GAGTC	2 cut(s)	1090, 1821
PluTI	GGCGCC	2 cut(s)	1542, 1958
PpsI	GAGTC	2 cut(s)	1090, 1821
PpuMI	RGGWCCY	2 cut(s)	121, 873
PshBI	ATTAAT	1 cut(s)	1662
Psp1406I	AACGTT	1 cut(s)	1123
Psp5II	RGGWCCY	2 cut(s)	121, 873
Psp6I	CCWGG	5 cut(s)	614, 1270, 1321, 1705, 1789
PspFI	CCCAGC	2 cut(s)	530, 2020
PspGI	CCWGG	5 cut(s)	614, 1270, 1321, 1705, 1789
PspPI	GGNCC	7 cut(s)	121, 202, 873, 1274, 1403, 1760, 1802
PspPPI	RGGWCCY	2 cut(s)	121, 873
PspXI	VCTCGAGB	2 cut(s)	125, 545
PstNI	CAGNNNCTG	2 cut(s)	775, 1790
PsuI	RGATCY	2 cut(s)	1807, 1942
PvuI	CGATCG	1 cut(s)	1682
PvuII	CAGCTG	2 cut(s)	1198, 1493
RsaI	GTAC	5 cut(s)	993, 1315, 1528, 1711, 1773
RsaNI	GTAC	5 cut(s)	992, 1314, 1527, 1710, 1772
SalI	GTCGAC	2 cut(s)	14, 406
SaqAI	TTAA	8 cut(s)	255, 513, 669, 1002, 1013, 1068, 1662, 1733
SatI	GCNGC	5 cut(s)	142, 1104, 1196, 1289, 1380
Sau3AI	GATC	6 cut(s)	678, 844, 1679, 1807, 1874, 1942
Sau96I	GGNCC	7 cut(s)	121, 202, 873, 1274, 1403, 1760, 1802
ScaI	AGTACT	1 cut(s)	1528
SchI	GAGTC	2 cut(s)	1091, 1822
ScrFI	CCNGG	6 cut(s)	616, 1272, 1323, 1707, 1791, 1973
SduI	GDGCHC	4 cut(s)	11, 135, 546, 1270
SfaNI	GCATC	8 cut(s)	153, 517, 769, 931, 1021, 1363, 1577, 1887
SfcI	CTRYAG	1 cut(s)	1936
SfoI	GGCGCC	2 cut(s)	1540, 1956
Sfr274I	CTCGAG	3 cut(s)	125, 545, 1740
SfuI	TTCGAA	1 cut(s)	308
SinI	GGWCC	5 cut(s)	121, 873, 1403, 1760, 1802
SlaI	CTCGAG	3 cut(s)	125, 545, 1740
SmlI	CTYRAG	5 cut(s)	74, 125, 512, 545, 1740
SmoI	CTYRAG	5 cut(s)	74, 125, 512, 545, 1740
Sse9I	AATT	7 cut(s)	151, 245, 250, 262, 278, 299, 353
SsiI	CCGC	8 cut(s)	139, 221, 327, 934, 1289, 1520, 1946, 1976
SspDI	GGCGCC	2 cut(s)	1538, 1954
SspI	AATATT	1 cut(s)	1163
SspMI	CTAG	1 cut(s)	539
StyD4I	CCNGG	6 cut(s)	614, 1270, 1321, 1705, 1789, 1971
StyI	CCWWGG	1 cut(s)	877
TaaI	ACNGT	9 cut(s)	13, 809, 972, 1390, 1414, 1463, 1869, 1919, 2007
TaiI	ACGT	2 cut(s)	1126, 1315
TaqII	GACCGA	1 cut(s)	1589
TasI	AATT	7 cut(s)	151, 245, 250, 262, 278, 299, 353
TatI	WGTACW	2 cut(s)	1526, 1771
TauI	GCSGC	1 cut(s)	1291
TfiI	GAWTC	5 cut(s)	314, 359, 575, 951, 1634
Tru1I	TTAA	8 cut(s)	255, 513, 669, 1002, 1013, 1068, 1662, 1733
Tru9I	TTAA	8 cut(s)	255, 513, 669, 1002, 1013, 1068, 1662, 1733
TscAI	CASTG	7 cut(s)	558, 613, 669, 814, 1566, 1804, 1996
TseFI	GTSAC	2 cut(s)	290, 322
TseI	GCWGC	4 cut(s)	141, 1103, 1195, 1379
Tsp45I	GTSAC	2 cut(s)	290, 322
TspDTI	ATGAA	4 cut(s)	710, 895, 1009, 1585
TspGWI	ACGGA	4 cut(s)	352, 558, 1040, 1752
TspRI	CASTG	7 cut(s)	558, 613, 669, 814, 1566, 1804, 1996
Vha464I	CTTAAG	1 cut(s)	512
VpaK11BI	GGWCC	5 cut(s)	121, 873, 1403, 1760, 1802
VspI	ATTAAT	1 cut(s)	1662
XapI	RAATTY	1 cut(s)	250
XhoI	CTCGAG	3 cut(s)	125, 545, 1740
XmiI	GTMKAC	3 cut(s)	15, 407, 1843
XspI	CTAG	1 cut(s)	539
ZrmI	AGTACT	1 cut(s)	1528

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.