Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

OG0012633

Physical Location & Seq

Genomic Coordinates Forward (+)

20943498 .. 20947054

Gene Structure 3557 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom04g19180.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGTCGATCACCGTCGACAAAATGTCCGGCGACCTCCACATGACGGCGGAGCTCCGCAAATCGTTCAACCTCCTCAAGACGCACGCCGCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACGTCGAGGATCACTTCCACTCCATCGACGATTCCATCCAGTCCGAGCTCGACCAACTGCAAAAACAACAATCGGAAGAGACCCATCTCACTCCGGATCAGCAATCGGAACCCAAAGAACCCGAATTGAATTTCAAACCCAATTCTGAAGGACGCCAATTGAGTTCCGGTGGCGCCCAATTGAGTTCGAAAGAATCCCAACTCACCGCCACCCTCCAATCCTTGAAAACCCAATTCGATTCCGTCGAAACCCAAGTCAAAACCGATGAGCGTTTGCCGGAAATGTCAACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTATGTGAATGAGCATTTGAAAGAGAGGGATTCGGTGCGTGACCGTGTTTCGGATGCGCTTAAGGTTTCTGTGGATGCTGGGAAGCTAGTGCTTGAGGCATTGAGATGGTTTTATCCATCGGAGTTGAATAAGGGAGGTAAGGAGCTTGATGTCACTGTTACTAGGAAAAGTTGTGTGATTTTGTTACAAGAGCTGGTGAATGTGAAACCAGTGATTAAACCCGATGTCAGCAGAGAGGCACTGAAGCTGGCATTGGCTTGGAAGGTGAAGGTGATGATGACTCAAATGTCGAACTCTTTGGAGGTTTGGGCTTTTTTGCAGCTTTTGGATACTTTTCAGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGATGTTTGTTAGTTGCGTAGCGGAGCGGAATTATGCACCAGAGTTGTTTAGGTCTCTTGGTTTCACCGATAAAGCCTCTGATCTCATCCAAAAGCTTGTGTCAGAGAATAAGCGCCTTGATGCTGTTAGATTCAGTCATGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCGTCACCAAAGATGCAAGGACAAGTTCCAAGAGGGGCCAAGAGCGTCTTAAAGCACAGGATGAGGCTAGAGACAAAGAAATAGCTGCTCTAAGAGGTATAATACGATGCATTGAGGCCTACAAGCTGCTTGTTGAACCACAATATTCTCCTGAGACCCTCAGAAAACGCATCAAGCAGCTGAGACGGCAGAGAAAAGAGAGGAAAGCAACTCTGACATCTCCTGGCCCTGAGGCTCAACCGCAATCCCTGAGTGGAAAGAAACGTACTGCCCCGCCTCAATCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCCAGCTTTAGCTGCTCAAACTCTTTCTTTCAGAGGACCAGCCACAGTACGCTCTGTACAACCATCTTACATTCGGCAAGTAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGTTATCATCCGCAGGAATGTCGGGACAAGCAACGACTCCTAATACTTCTTATGGCACCATTTCCACCTTGAACCCATTGCGTGCATCTCATCATCAGCAAGGAGGCTCATTCATGGGTCAAGGTGCAGAATACTCTGCTCGATACTATGATTCAGCGCACTACGCTCCTGACATTAATAATGACTTATCTGCTCGATCATATGACTTGGTGGGCTCCTCTCCTGGTACCCGTACCCAGCACTTGAACTTGACAGCTCGAGACTATGGATTGACGGCTCCGTCTCATGTACATACGAACTCAGCAATTGCATCTTATGGACTCGGGGGCAGTAGTCATGTAACTTCAGATATAAGCCCATTAGTCGCACGATACGGGGCAAGTATAGCTGCAAATGGAAGGACTGGACGGTTTGGATCGGCTGGAAGCCGTACACCTGTGCGCAATGCAAGCCCGAATAGTTCCATCCCTTATGCGTATAGGGACCATGCAAGCCCGAATAGTTCCATCCCTTATGCGTATAGGGATCCGGTGAGGGCACCTAACCACAACGACCGGTCAGTGCCTTCCAGCAGTGGCCATGGCGCGCCGTCTCAGCACCCCCCGGCCATTTATCACCTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Frigida

Protein Analysis

692

Amino Acids

76.45

Weight (kDa)

9.29

Isoelectric Point (pI)

53.7

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g04240 FvH4_6g04240
malus_domestica	MD00G1051400.v1.1 MD12G1231700.v1.1 MD12G1232000.v1.1
prunus_persica	Prupe.6G334900_v2.0.a1
pyrus_communis	pycom04g19180 pycom12g21440
rosa_chinensis	RchiOBHm_Chr3g0452911
rosa_laevigata	RLG00000025560
rosa_multiflora	Rmu_sc0002823.1_g000005 Rmu_sc0002823.1_g000006
rosa_roxburghii	Rroxscaffold_6G00429920
rosa_rugosa	Rorug02G0643600
rosa_samantha	Rh3AG046400 Rh3BG048900 Rh3CG047500 Rh3DG048500
rosa_wichuraiana	Rw3G003650

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	739, 1732
AatII	GACGTC	1 cut(s)	126
Acc16I	TGCGCA	1 cut(s)	1895
Acc36I	ACCTGC	1 cut(s)	419
Acc65I	GGTACC	1 cut(s)	1679
AccB1I	GGYRCC	4 cut(s)	293, 1508, 1679, 1990
AccBSI	CCGCTC	1 cut(s)	850
AccI	GTMKAC	1 cut(s)	15
AccII	CGCG	1 cut(s)	2039
AccIII	TCCGGA	1 cut(s)	214
AciI	CCGC	9 cut(s)	47, 55, 87, 327, 845, 850, 1262, 1295, 1464
AclWI	GGATC	5 cut(s)	138, 225, 1876, 1973, 1986
AcoI	YGGCCR	2 cut(s)	2029, 2058
AcsI	RAATTY	1 cut(s)	250
AcuI	CTGAAG	3 cut(s)	288, 716, 1782
AcyI	GRCGYC	4 cut(s)	123, 274, 294, 420
AfaI	GTAC	8 cut(s)	996, 1288, 1389, 1398, 1681, 1687, 1743, 1885
AfiI	CCNNNNNNNGG	4 cut(s)	43, 114, 118, 502
AflII	CTTAAG	1 cut(s)	512
AgeI	ACCGGT	1 cut(s)	2007
AgsI	TTSAA	9 cut(s)	67, 250, 256, 346, 472, 580, 1157, 1525, 1699
AhdI	GACNNNNNGTC	1 cut(s)	22
AjnI	CCWGG	2 cut(s)	1243, 1675
AjuI	GAANNNNNNNTTGG	2 cut(s)	689, 721
Alw21I	GWGCWC	2 cut(s)	54, 171
Alw26I	GTCTC	8 cut(s)	194, 882, 1086, 1169, 1198, 1707, 1740, 2049
AlwI	GGATC	5 cut(s)	138, 225, 1876, 1973, 1986
Ama87I	CYCGRG	2 cut(s)	1710, 1775
Aor13HI	TCCGGA	1 cut(s)	214
AoxI	GGCC	5 cut(s)	1057, 1137, 1246, 2029, 2058
ApeKI	GCWGC	6 cut(s)	772, 1106, 1147, 1198, 1352, 1841
ApoI	RAATTY	1 cut(s)	250
ArsI	GACNNNNNNTTYG	2 cut(s)	360, 392
AscI	GGCGCGCC	1 cut(s)	2037
AseI	ATTAAT	1 cut(s)	1629
AsiGI	ACCGGT	1 cut(s)	2007
Asp718I	GGTACC	1 cut(s)	1679
AspLEI	GCGC	7 cut(s)	296, 511, 939, 1612, 1896, 2039, 2041
AspS9I	GGNCC	4 cut(s)	1057, 1247, 1376, 1936
AsuC2I	CCSGG	1 cut(s)	2057
AsuHPI	GGTGA	9 cut(s)	316, 661, 730, 736, 812, 880, 1018, 1996, 2060
AsuII	TTCGAA	1 cut(s)	308
AvaI	CYCGRG	2 cut(s)	1710, 1775
AvaII	GGWCC	2 cut(s)	1376, 1936
AxyI	CCTNAGG	1 cut(s)	1251
BaeGI	GKGCMC	1 cut(s)	1993
BalI	TGGCCA	1 cut(s)	2031
BamHI	GGATCC	1 cut(s)	1978
BanI	GGYRCC	4 cut(s)	293, 1508, 1679, 1990
BanII	GRGCYC	3 cut(s)	54, 171, 1670
Bbv12I	GWGCWC	2 cut(s)	54, 171
BbvI	GCAGC	6 cut(s)	784, 1093, 1134, 1210, 1339, 1828
BccI	CCATC	8 cut(s)	152, 164, 213, 552, 577, 1411, 1925, 1967
BceAI	ACGGC	6 cut(s)	60, 451, 1223, 1743, 1866, 2026
BcgI	CGANNNNNNTGC	2 cut(s)	1455, 1489
BciT130I	CCWGG	2 cut(s)	1245, 1677
BciVI	GTATCC	1 cut(s)	775
BcnI	CCSGG	1 cut(s)	2057
BcoDI	GTCTC	8 cut(s)	194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BfaI	CTAG	3 cut(s)	539, 615, 1089
BfoI	RGCGCY	2 cut(s)	297, 940
BfrI	CTTAAG	1 cut(s)	512
BfuAI	ACCTGC	1 cut(s)	419
BfuI	GTATCC	1 cut(s)	775
BisI	GCNGC	7 cut(s)	87, 773, 1107, 1148, 1199, 1353, 1842
BlsI	GCNGC	7 cut(s)	88, 774, 1108, 1149, 1200, 1354, 1843
Bme1390I	CCNGG	3 cut(s)	1245, 1677, 2057
Bme18I	GGWCC	2 cut(s)	1376, 1936
BmeRI	GACNNNNNGTC	1 cut(s)	22
BmeT110I	CYCGRG	2 cut(s)	1710, 1775
BmgT120I	GGNCC	4 cut(s)	1057, 1247, 1376, 1936
BmrFI	CCNGG	3 cut(s)	1245, 1677, 2057
BmsI	GCATC	9 cut(s)	496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
BpmI	CTGGAG	1 cut(s)	1326
Bpu14I	TTCGAA	1 cut(s)	308
BpuEI	CTTGAG	2 cut(s)	59, 566
BpuMI	CCSGG	1 cut(s)	2057
BsaHI	GRCGYC	4 cut(s)	123, 274, 294, 420
BsaI	GGTCTC	3 cut(s)	194, 882, 1169
BsaJI	CCNNGG	2 cut(s)	2032, 2055
BsaWI	WCCGGW	4 cut(s)	214, 287, 1981, 2007
BsaXI	ACNNNNNCTCC	2 cut(s)	125, 155
Bsc4I	CCNNNNNNNGG	4 cut(s)	43, 114, 118, 502
Bse118I	RCCGGY	1 cut(s)	2007
Bse1I	ACTGG	3 cut(s)	160, 662, 1861
Bse21I	CCTNAGG	1 cut(s)	1251
Bse3DI	GCAATG	3 cut(s)	421, 1529, 1903
BseAI	TCCGGA	1 cut(s)	214
BseBI	CCWGG	2 cut(s)	1245, 1677
BseDI	CCNNGG	2 cut(s)	2032, 2055
BseGI	GGATG	9 cut(s)	156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BseLI	CCNNNNNNNGG	4 cut(s)	43, 114, 118, 502
BseMI	GCAATG	3 cut(s)	421, 1529, 1903
BseMII	CTCAG	7 cut(s)	1164, 1193, 1195, 1242, 1262, 1767, 2060
BseNI	ACTGG	3 cut(s)	160, 662, 1861
BsePI	GCGCGC	1 cut(s)	2037
BseRI	GAGGAG	2 cut(s)	62, 1660
BseSI	GKGCMC	1 cut(s)	1993
BseXI	GCAGC	6 cut(s)	784, 1093, 1134, 1210, 1339, 1828
BseYI	CCCAGC	2 cut(s)	530, 1689
BsgI	GTGCAG	2 cut(s)	1464, 1599
Bsh1236I	CGCG	1 cut(s)	2039
Bsh1285I	CGRYCG	1 cut(s)	2008
BshFI	GGCC	5 cut(s)	1059, 1139, 1248, 2031, 2060
BshNI	GGYRCC	4 cut(s)	293, 1508, 1679, 1990
BshTI	ACCGGT	1 cut(s)	2007
BsiEI	CGRYCG	1 cut(s)	2008
BsiHKAI	GWGCWC	2 cut(s)	54, 171
BsiHKCI	CYCGRG	2 cut(s)	1710, 1775
BsiSI	CCGG	7 cut(s)	27, 215, 288, 398, 1982, 2008, 2057
BslFI	GGGAC	3 cut(s)	407, 1491, 1949
BslI	CCNNNNNNNGG	4 cut(s)	43, 114, 118, 502
BsmAI	GTCTC	8 cut(s)	194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BsmBI	CGTCTC	3 cut(s)	1198, 1740, 2049
BsmFI	GGGAC	3 cut(s)	407, 1491, 1949
BsnI	GGCC	5 cut(s)	1059, 1139, 1248, 2031, 2060
Bso31I	GGTCTC	3 cut(s)	194, 882, 1169
BsoBI	CYCGRG	2 cut(s)	1710, 1775
Bsp119I	TTCGAA	1 cut(s)	308
Bsp1286I	GDGCHC	4 cut(s)	54, 171, 1670, 1993
Bsp13I	TCCGGA	1 cut(s)	214
Bsp1407I	TGTACA	2 cut(s)	1396, 1741
Bsp143I	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
Bsp19I	CCATGG	1 cut(s)	2032
BspACI	CCGC	9 cut(s)	47, 55, 87, 327, 845, 850, 1262, 1295, 1464
BspANI	GGCC	5 cut(s)	1059, 1139, 1248, 2031, 2060
BspCNI	CTCAG	7 cut(s)	1165, 1194, 1194, 1243, 1263, 1766, 2059
BspEI	TCCGGA	1 cut(s)	214
BspFNI	CGCG	1 cut(s)	2039
BspMI	ACCTGC	1 cut(s)	419
BspPI	GGATC	5 cut(s)	138, 225, 1876, 1973, 1986
BspT104I	TTCGAA	1 cut(s)	308
BspT107I	GGYRCC	4 cut(s)	293, 1508, 1679, 1990
BspTI	CTTAAG	1 cut(s)	512
BspTNI	GGTCTC	3 cut(s)	194, 882, 1169
BsrBI	CCGCTC	1 cut(s)	850
BsrDI	GCAATG	3 cut(s)	421, 1529, 1903
BsrFI	RCCGGY	1 cut(s)	2007
BsrGI	TGTACA	2 cut(s)	1396, 1741
BsrI	ACTGG	3 cut(s)	160, 662, 1861
BssAI	RCCGGY	1 cut(s)	2007
BssECI	CCNNGG	2 cut(s)	2032, 2055
BssHII	GCGCGC	1 cut(s)	2037
BssMI	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
BssNI	GRCGYC	4 cut(s)	123, 274, 294, 420
BssT1I	CCWWGG	1 cut(s)	2032
Bst2UI	CCWGG	2 cut(s)	1245, 1677
Bst4CI	ACNGT	8 cut(s)	13, 497, 610, 812, 975, 1387, 1436, 1863
Bst6I	CTCTTC	1 cut(s)	192
BstACI	GRCGYC	4 cut(s)	123, 274, 294, 420
BstAFI	CTTAAG	1 cut(s)	512
BstAUI	TGTACA	2 cut(s)	1396, 1741
BstBI	TTCGAA	1 cut(s)	308
BstC8I	GCNNGC	6 cut(s)	84, 702, 1536, 1903, 1945, 2039
BstDEI	CTNAG	8 cut(s)	1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
BstDSI	CCRYGG	1 cut(s)	2032
BstF5I	GGATG	9 cut(s)	156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BstFNI	CGCG	1 cut(s)	2039
BstH2I	RGCGCY	2 cut(s)	297, 940
BstHHI	GCGC	7 cut(s)	296, 511, 939, 1612, 1896, 2039, 2041
BstKTI	GATC	7 cut(s)	9, 133, 220, 907, 1652, 1871, 1981
BstMAI	GTCTC	8 cut(s)	194, 882, 1086, 1169, 1198, 1707, 1740, 2049
BstMBI	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
BstMCI	CGRYCG	1 cut(s)	2008
BstMWI	GCNNNNNNNGC	7 cut(s)	95, 1207, 1352, 1616, 1838, 1902, 2047
BstNI	CCWGG	2 cut(s)	1245, 1677
BstSCI	CCNGG	3 cut(s)	1243, 1675, 2055
BstSLI	GKGCMC	1 cut(s)	1993
BstUI	CGCG	1 cut(s)	2039
BstV1I	GCAGC	6 cut(s)	784, 1093, 1134, 1210, 1339, 1828
BstX2I	RGATCY	1 cut(s)	1978
BstYI	RGATCY	1 cut(s)	1978
Bsu36I	CCTNAGG	1 cut(s)	1251
BsuI	GTATCC	1 cut(s)	775
BsuRI	GGCC	5 cut(s)	1059, 1139, 1248, 2031, 2060
BtgI	CCRYGG	1 cut(s)	2032
BtsCI	GGATG	9 cut(s)	156, 511, 532, 909, 1087, 1327, 1460, 1917, 1959
BtsI	GCAGTG	1 cut(s)	2032
BtsIMutI	CAGTG	6 cut(s)	606, 669, 692, 817, 2019, 2032
BveI	ACCTGC	1 cut(s)	419
Cac8I	GCNNGC	6 cut(s)	84, 702, 1536, 1903, 1945, 2039
CfoI	GCGC	7 cut(s)	296, 511, 939, 1612, 1896, 2039, 2041
Cfr10I	RCCGGY	1 cut(s)	2007
Cfr13I	GGNCC	4 cut(s)	1057, 1247, 1376, 1936
CseI	GACGC	4 cut(s)	88, 282, 409, 1055
Csp6I	GTAC	8 cut(s)	995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
CspAI	ACCGGT	1 cut(s)	2007
CviAII	CATG	7 cut(s)	40, 962, 1567, 1739, 1790, 1940, 2033
CviQI	GTAC	8 cut(s)	995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
DdeI	CTNAG	8 cut(s)	1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
DinI	GGCGCC	1 cut(s)	295
DpnI	GATC	7 cut(s)	8, 132, 219, 906, 1651, 1870, 1980
DpnII	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
DraI	TTTAAA	1 cut(s)	1017
DrdI	GACNNNNNNGTC	2 cut(s)	739, 1732
DriI	GACNNNNNGTC	1 cut(s)	22
DseDI	GACNNNNNNGTC	2 cut(s)	739, 1732
EaeI	YGGCCR	2 cut(s)	2029, 2058
Eam1104I	CTCTTC	1 cut(s)	192
Eam1105I	GACNNNNNGTC	1 cut(s)	22
EarI	CTCTTC	1 cut(s)	192
EciI	GGCGGA	1 cut(s)	62
Ecl136II	GAGCTC	2 cut(s)	52, 169
Eco130I	CCWWGG	1 cut(s)	2032
Eco147I	AGGCCT	1 cut(s)	1139
Eco24I	GRGCYC	3 cut(s)	54, 171, 1670
Eco31I	GGTCTC	3 cut(s)	194, 882, 1169
Eco47I	GGWCC	2 cut(s)	1376, 1936
Eco53kI	GAGCTC	2 cut(s)	52, 169
Eco57I	CTGAAG	3 cut(s)	288, 716, 1782
Eco81I	CCTNAGG	1 cut(s)	1251
Eco88I	CYCGRG	2 cut(s)	1710, 1775
EcoICRI	GAGCTC	2 cut(s)	52, 169
EcoRII	CCWGG	2 cut(s)	1243, 1675
EcoT14I	CCWWGG	1 cut(s)	2032
EcoT22I	ATGCAT	2 cut(s)	967, 1133
EcoT38I	GRGCYC	3 cut(s)	54, 171, 1670
EgeI	GGCGCC	1 cut(s)	295
EheI	GGCGCC	1 cut(s)	295
ErhI	CCWWGG	1 cut(s)	2032
Esp3I	CGTCTC	3 cut(s)	1198, 1740, 2049
FaeI	CATG	7 cut(s)	43, 965, 1570, 1742, 1793, 1943, 2036
FalI	AAGNNNNNCTT	2 cut(s)	1053, 1085
FaqI	GGGAC	3 cut(s)	407, 1491, 1949
FatI	CATG	7 cut(s)	39, 961, 1566, 1738, 1789, 1939, 2032
FauI	CCCGC	1 cut(s)	1302
FauNDI	CATATG	1 cut(s)	1654
FblI	GTMKAC	1 cut(s)	15
Fnu4HI	GCNGC	7 cut(s)	87, 773, 1107, 1148, 1199, 1353, 1842
FokI	GGATG	9 cut(s)	143, 518, 539, 896, 1094, 1314, 1447, 1904, 1946
FriOI	GRGCYC	3 cut(s)	54, 171, 1670
Fsp4HI	GCNGC	7 cut(s)	87, 773, 1107, 1148, 1199, 1353, 1842
FspBI	CTAG	3 cut(s)	539, 615, 1089
FspI	TGCGCA	1 cut(s)	1895
GlaI	GCGC	7 cut(s)	295, 510, 938, 1611, 1895, 2038, 2040
GluI	GCNGC	7 cut(s)	87, 773, 1107, 1148, 1199, 1353, 1842
GsaI	CCCAGC	2 cut(s)	534, 1693
GsuI	CTGGAG	1 cut(s)	1326
HaeII	RGCGCY	2 cut(s)	297, 940
HaeIII	GGCC	5 cut(s)	1059, 1139, 1248, 2031, 2060
HapII	CCGG	7 cut(s)	27, 215, 288, 398, 1982, 2008, 2057
HgaI	GACGC	4 cut(s)	88, 282, 409, 1055
HhaI	GCGC	7 cut(s)	296, 511, 939, 1612, 1896, 2039, 2041
Hin1I	GRCGYC	4 cut(s)	123, 274, 294, 420
Hin1II	CATG	7 cut(s)	43, 965, 1570, 1742, 1793, 1943, 2036
Hin6I	GCGC	7 cut(s)	294, 509, 937, 1610, 1894, 2037, 2039
HinP1I	GCGC	7 cut(s)	294, 509, 937, 1610, 1894, 2037, 2039
HincII	GTYRAC	2 cut(s)	16, 408
HindII	GTYRAC	2 cut(s)	16, 408
HindIII	AAGCTT	2 cut(s)	917, 1423
HinfI	GANTC	9 cut(s)	152, 314, 359, 482, 733, 954, 1489, 1604, 1773
HpaII	CCGG	7 cut(s)	27, 215, 288, 398, 1982, 2008, 2057
HphI	GGTGA	9 cut(s)	316, 661, 730, 736, 812, 880, 1018, 1996, 2060
Hpy166II	GTNNAC	3 cut(s)	16, 408, 1886
Hpy188III	TCNNGA	7 cut(s)	76, 215, 424, 1172, 1476, 1622, 1712
Hpy8I	GTNNAC	3 cut(s)	16, 408, 1886
Hpy99I	CGWCG	4 cut(s)	17, 128, 152, 368
HpyAV	CCTTC	5 cut(s)	263, 709, 715, 1845, 2028
HpyCH4III	ACNGT	8 cut(s)	13, 497, 610, 812, 975, 1387, 1436, 1863
HpyCH4IV	ACGT	2 cut(s)	123, 1285
HpyF10VI	GCNNNNNNNGC	7 cut(s)	95, 1207, 1352, 1616, 1838, 1902, 2047
HpyF3I	CTNAG	8 cut(s)	1112, 1173, 1181, 1202, 1251, 1271, 1753, 2046
HpySE526I	ACGT	2 cut(s)	123, 1285
Hsp92I	GRCGYC	4 cut(s)	123, 274, 294, 420
Hsp92II	CATG	7 cut(s)	43, 965, 1570, 1742, 1793, 1943, 2036
HspAI	GCGC	7 cut(s)	294, 509, 937, 1610, 1894, 2037, 2039
KasI	GGCGCC	1 cut(s)	293
Kpn2I	TCCGGA	1 cut(s)	214
KpnI	GGTACC	1 cut(s)	1683
Kzo9I	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
LmnI	GCTCC	8 cut(s)	49, 57, 595, 847, 1345, 1624, 1673, 1735
Lsp1109I	GCAGC	6 cut(s)	784, 1093, 1134, 1210, 1339, 1828
LweI	GCATC	9 cut(s)	496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
MaeI	CTAG	3 cut(s)	539, 615, 1089
MaeII	ACGT	2 cut(s)	123, 1285
MaeIII	GTNAC	6 cut(s)	491, 604, 610, 636, 1024, 1792
MalI	GATC	7 cut(s)	8, 132, 219, 906, 1651, 1870, 1980
MbiI	CCGCTC	1 cut(s)	850
MboI	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
MboII	GAAGA	1 cut(s)	209
MfeI	CAATTG	3 cut(s)	278, 299, 1758
MflI	RGATCY	1 cut(s)	1978
MhlI	GDGCHC	4 cut(s)	54, 171, 1670, 1993
MlsI	TGGCCA	1 cut(s)	2031
MluCI	AATT	8 cut(s)	245, 250, 262, 278, 299, 353, 853, 1758
MluNI	TGGCCA	1 cut(s)	2031
Mly113I	GGCGCC	1 cut(s)	294
MlyI	GAGTC	3 cut(s)	727, 1483, 1767
Mox20I	TGGCCA	1 cut(s)	2031
Mph1103I	ATGCAT	2 cut(s)	967, 1133
MroI	TCCGGA	1 cut(s)	214
MscI	TGGCCA	1 cut(s)	2031
MseI	TTAA	6 cut(s)	513, 669, 1005, 1016, 1071, 1629
MslI	CAYNNNNRTG	1 cut(s)	556
Msp20I	TGGCCA	1 cut(s)	2031
MspA1I	CMGCKG	1 cut(s)	1201
MspCI	CTTAAG	1 cut(s)	512
MspI	CCGG	7 cut(s)	27, 215, 288, 398, 1982, 2008, 2057
MspR9I	CCNGG	3 cut(s)	1245, 1677, 2057
MunI	CAATTG	3 cut(s)	278, 299, 1758
MvaI	CCWGG	2 cut(s)	1245, 1677
MvnI	CGCG	1 cut(s)	2039
MwoI	GCNNNNNNNGC	7 cut(s)	95, 1207, 1352, 1616, 1838, 1902, 2047
NarI	GGCGCC	1 cut(s)	294
NciI	CCSGG	1 cut(s)	2057
NcoI	CCATGG	1 cut(s)	2032
NdeI	CATATG	1 cut(s)	1654
NdeII	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
NlaIII	CATG	7 cut(s)	43, 965, 1570, 1742, 1793, 1943, 2036
NmuCI	GTSAC	3 cut(s)	491, 604, 1024
NsbI	TGCGCA	1 cut(s)	1895
NsiI	ATGCAT	2 cut(s)	967, 1133
NspV	TTCGAA	1 cut(s)	308
PaeR7I	CTCGAG	1 cut(s)	1710
PalAI	GGCGCGCC	1 cut(s)	2037
PauI	GCGCGC	1 cut(s)	2037
PceI	AGGCCT	1 cut(s)	1139
PcsI	WCGNNNNNNNCGW	1 cut(s)	363
PfeI	GAWTC	6 cut(s)	152, 314, 359, 482, 954, 1604
PinAI	ACCGGT	1 cut(s)	2007
PkrI	GCNGC	7 cut(s)	88, 774, 1108, 1149, 1200, 1354, 1843
PleI	GAGTC	3 cut(s)	727, 1483, 1767
PluTI	GGCGCC	1 cut(s)	297
PpsI	GAGTC	3 cut(s)	727, 1483, 1767
PshBI	ATTAAT	1 cut(s)	1629
Psp124BI	GAGCTC	2 cut(s)	54, 171
Psp6I	CCWGG	2 cut(s)	1243, 1675
PspFI	CCCAGC	2 cut(s)	530, 1689
PspGI	CCWGG	2 cut(s)	1243, 1675
PspPI	GGNCC	4 cut(s)	1057, 1247, 1376, 1936
PsuI	RGATCY	1 cut(s)	1978
PteI	GCGCGC	1 cut(s)	2037
PvuII	CAGCTG	1 cut(s)	1201
RsaI	GTAC	8 cut(s)	996, 1288, 1389, 1398, 1681, 1687, 1743, 1885
RsaNI	GTAC	8 cut(s)	995, 1287, 1388, 1397, 1680, 1686, 1742, 1884
RseI	CAYNNNNRTG	1 cut(s)	556
SacI	GAGCTC	2 cut(s)	54, 171
SalI	GTCGAC	1 cut(s)	14
SaqAI	TTAA	6 cut(s)	513, 669, 1005, 1016, 1071, 1629
SatI	GCNGC	7 cut(s)	87, 773, 1107, 1148, 1199, 1353, 1842
Sau3AI	GATC	7 cut(s)	6, 130, 217, 904, 1649, 1868, 1978
Sau96I	GGNCC	4 cut(s)	1057, 1247, 1376, 1936
SchI	GAGTC	3 cut(s)	727, 1483, 1767
ScrFI	CCNGG	3 cut(s)	1245, 1677, 2057
SduI	GDGCHC	4 cut(s)	54, 171, 1670, 1993
SfaNI	GCATC	9 cut(s)	496, 517, 934, 1024, 1118, 1200, 1336, 1547, 1772
SfoI	GGCGCC	1 cut(s)	295
Sfr274I	CTCGAG	1 cut(s)	1710
SfuI	TTCGAA	1 cut(s)	308
SgsI	GGCGCGCC	1 cut(s)	2037
SinI	GGWCC	2 cut(s)	1376, 1936
SlaI	CTCGAG	1 cut(s)	1710
SmiMI	CAYNNNNRTG	1 cut(s)	556
SmlI	CTYRAG	4 cut(s)	74, 512, 545, 1710
SmoI	CTYRAG	4 cut(s)	74, 512, 545, 1710
Sse9I	AATT	8 cut(s)	245, 250, 262, 278, 299, 353, 853, 1758
SseBI	AGGCCT	1 cut(s)	1139
SsiI	CCGC	9 cut(s)	47, 55, 87, 327, 845, 850, 1262, 1295, 1464
SspDI	GGCGCC	1 cut(s)	293
SspI	AATATT	1 cut(s)	1166
SspMI	CTAG	3 cut(s)	539, 615, 1089
SstI	GAGCTC	2 cut(s)	54, 171
StuI	AGGCCT	1 cut(s)	1139
StyD4I	CCNGG	3 cut(s)	1243, 1675, 2055
StyI	CCWWGG	1 cut(s)	2032
TaaI	ACNGT	8 cut(s)	13, 497, 610, 812, 975, 1387, 1436, 1863
TaiI	ACGT	2 cut(s)	126, 1288
TasI	AATT	8 cut(s)	245, 250, 262, 278, 299, 353, 853, 1758
TatI	WGTACW	2 cut(s)	1396, 1741
TauI	GCSGC	1 cut(s)	89
TfiI	GAWTC	6 cut(s)	152, 314, 359, 482, 954, 1604
Tru1I	TTAA	6 cut(s)	513, 669, 1005, 1016, 1071, 1629
Tru9I	TTAA	6 cut(s)	513, 669, 1005, 1016, 1071, 1629
TscAI	CASTG	6 cut(s)	613, 669, 699, 817, 2019, 2032
TseFI	GTSAC	3 cut(s)	491, 604, 1024
TseI	GCWGC	6 cut(s)	772, 1106, 1147, 1198, 1352, 1841
Tsp45I	GTSAC	3 cut(s)	491, 604, 1024
TspDTI	ATGAA	1 cut(s)	1555
TspGWI	ACGGA	2 cut(s)	352, 1722
TspRI	CASTG	6 cut(s)	613, 669, 699, 817, 2019, 2032
Vha464I	CTTAAG	1 cut(s)	512
VpaK11BI	GGWCC	2 cut(s)	1376, 1936
VspI	ATTAAT	1 cut(s)	1629
XapI	RAATTY	1 cut(s)	250
XhoI	CTCGAG	1 cut(s)	1710
XmiI	GTMKAC	1 cut(s)	15
XspI	CTAG	3 cut(s)	539, 615, 1089
ZraI	GACGTC	1 cut(s)	124
Zsp2I	ATGCAT	2 cut(s)	967, 1133

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.