Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0007826

Physical Location & Seq

Genomic Coordinates Reverse (-)

10514124 .. 10520859

Gene Structure 6736 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1056100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGCCGTTGCGGTCGGCAACTGCTGCTTCTCTGCGGTCACCTCCACCGTTAAACTCCGCTCCTCCGTCTCCGTCCAACCTTTCTTCGCCACGTGGCTCTGTTCCGGCAGCTACCAGTACCACGCTCTTAAGCCCGTCGGCCAAAGCAACAGAGAAAGAGCTTCCAGTTCGAGAAAAGGCTCGCAGTCGTCGTCGCCGCCGTCCGCTGATAAATACCAACCGGAGAGAGAAGGCGTTCCCAGTAAGTTTAGCGGAAATGAGAGAGGGAAGAAGGGGAAGTCCCAATCGGTGGCGTACAGAGCGTTCGGAACGCAACGAAAAGAGAGGAAGGAGTCCGTAGTTGATCAGAAAGAGCAGCAGGTTGAACCGAAAAGTCTCCAGGATGCTGATTTTCTCAATGCCGTTGTCAAGGTTTACTGCACCCATACCGCGCCTGACTACTCCCTTCCATGGCAAAAACAAAGACAATATACAAGTACAGGCAGTGCTTTTATGATTGGTGACGGGAAACTCTTGACAAATGCCCACTGTGTTGAGCATTATACTCAGGTCAAAGTGAAGAGAAGAGGGGATGATACCAAATTTGTGGCTAAGGTTTTGGCCAGAGGTGTTGATTGTGATATTGCTTTACTTTCGGTAGAAAGTGAGGAATTCTGGAAAGGAGCAGAACCACTCCAATTGGGAAGCTTGCCACATCTTCAGGAAGCAGTGACTGTTGTAGGATATCCCCTTGGAGGAGATACCATTTCAGTAACTAAGGGGGTTGTATCGCGTATAGAGGTTACACCATATGCTCATGGGTCATCTGATTTGCTGGGCATTCAAATTGATGCAGCAATAAACCCTGGTAATAGTGGAGGCCCTGCATTCAATGATCAAGGGGAGTGCATTGGTGTGGCATTTCAGGTCTACAGATCTGAAGAGGCCGAGAATATTGGATATGTCATTCCTACTACAGTTGTGTCTCATTTTTTGGATGACTATGAGAGGAATGGGAAATACACAGGTTTCCCTTGCCTTGGTGTATTGTTACAAAAGTTGGAGAATCCAGCATTAAAGGCATGCCTGAAAGTAGAGTCTATTGAGGGTGTACTGGTGCGAAGAGTTGAACCCACTTCTGATGCACATAATGTATTAAAGGAGGGGGACGTCATTGTAAGCTTTGATGATGTTCATGTAGGGTGTGAAGGAACAATACCCTTTCGATCAAATGAGCGCATCGCATTCCGCTACCTCATTAGCCAAAAATTTGCAGGCGACGTTGCAGATATTGGAATTATTAGAGCTGGAGAGTACATGAAAGTTAAAGCAGTTTTGCACCCACGAGTGCATTTGGTTCCCTTTCATATCGATGGAGGTCAGCCTTCTTACCTAATAATTGCTGGTTTGGTGTTTACCCCACTCTCAGAACCACTGATAGAAGAGGAGTGTGACGACTCTATAGGGCTTAAACTCTTGGCGAAGGCACGCTACTCTTTGGCTAGATTCAAAGGGGAACAGATTGTAATCCTATCACAGGTCTTAGCCAATGAAGTAAACATTGGGTATGAAGACATGAGCAATCAGCAGGTTTTGAAGTTAAACGGAATCCAAATACGGAACATTCATCATTTAGCCTACCTTGTAGACTCATGCAAGGACAAGTACTTAGTCTTTGAATTCGAAGACAACTACATAACCGTGTTGGAAAGGGAAGCAGCTGCTGCTGCTTCGTCCTGCATTCTCAAAGACTACGGCATTCCATCCGAAAGATCTTCCGATCTGTTGGAGCCCTACGCGGATTCGCTTGGGGACAACCAAGCAGTTGATCAGGATATCGGTGACAGTCCGGTTTCAAATTTGGAAATCGGCTTCGATGGAATTATTTGGGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

625

Amino Acids

68.38

Weight (kDa)

5.51

Isoelectric Point (pI)

42.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	140 - 322	3.4e-23	Trypsin
Trypsin_2	PF13365	161 - 299	6.1e-26	Trypsin-like peptidase domain
PDZ_3	PF17815	446 - 587	3.6e-45	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47940 AT2G47940
fragaria_vesca	FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740
malus_domestica	MD00G1056100.v1.1 MD11G1298100.v1.1
prunus_persica	Prupe.8G252400_v2.0.a1
pyrus_communis	pycom11g26180
rosa_chinensis	RchiOBHm_Chr6g0292561
rosa_laevigata	RLG00000012009
rosa_multiflora	Rmu_sc0000515.1_g000002
rosa_roxburghii	Rroxscaffold_7G00174930
rosa_rugosa	Rorug06G0228000 Rorug06G0228100
rosa_samantha	Rh6AG341200 Rh6BG348600 Rh6CG355500 Rh6DG341300
rosa_wichuraiana	Rw6G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1152
Acc36I	ACCTGC	2 cut(s)	349, 1558
AccB7I	CCANNNNNTGG	1 cut(s)	289
AccBSI	CCGCTC	1 cut(s)	60
AccI	GTMKAC	2 cut(s)	909, 1626
AccII	CGCG	3 cut(s)	431, 772, 1778
AciI	CCGC	9 cut(s)	11, 35, 58, 197, 204, 252, 429, 1228, 1778
AcoI	YGGCCR	3 cut(s)	3, 139, 600
AcsI	RAATTY	5 cut(s)	581, 650, 1247, 1658, 1837
AcuI	CTGAAG	2 cut(s)	683, 939
AcvI	CACGTG	1 cut(s)	93
AcyI	GRCGYC	1 cut(s)	1149
AfaI	GTAC	6 cut(s)	119, 296, 478, 1092, 1295, 1646
AfiI	CCNNNNNNNGG	5 cut(s)	289, 450, 734, 1019, 1516
AflII	CTTAAG	1 cut(s)	128
AgsI	TTSAA	8 cut(s)	365, 824, 871, 1109, 1489, 1576, 1658, 1836
AjnI	CCWGG	2 cut(s)	378, 844
AjuI	GAANNNNNNNTTGG	2 cut(s)	1524, 1556
AluBI	AGCT	6 cut(s)	111, 161, 687, 1161, 1286, 1700
AluI	AGCT	6 cut(s)	111, 161, 687, 1161, 1286, 1700
Alw26I	GTCTC	3 cut(s)	73, 380, 969
AlwNI	CAGNNNCTG	2 cut(s)	713, 1703
AoxI	GGCC	5 cut(s)	3, 139, 600, 859, 924
ApeKI	GCWGC	8 cut(s)	24, 108, 355, 833, 1697, 1700, 1703, 1706
ApoI	RAATTY	5 cut(s)	581, 650, 1247, 1658, 1837
Asp700I	GAANNNNTTC	1 cut(s)	234
AspLEI	GCGC	2 cut(s)	433, 1218
AspS9I	GGNCC	1 cut(s)	860
AsuHPI	GGTGA	3 cut(s)	31, 512, 1832
AsuII	TTCGAA	1 cut(s)	1662
BalI	TGGCCA	1 cut(s)	602
BanII	GRGCYC	1 cut(s)	1773
BarI	GAAGNNNNNNTAC	4 cut(s)	1179, 1211, 1454, 1486
BauI	CACGAG	1 cut(s)	1323
BbrPI	CACGTG	1 cut(s)	93
BbsI	GAAGAC	2 cut(s)	1557, 1671
BbvI	GCAGC	8 cut(s)	11, 120, 367, 845, 1687, 1690, 1693, 1709
BccI	CCATC	3 cut(s)	1346, 1750, 1850
BceAI	ACGGC	3 cut(s)	184, 386, 1750
BciT130I	CCWGG	2 cut(s)	380, 846
BclI	TGATCA	3 cut(s)	343, 874, 1807
BcoDI	GTCTC	3 cut(s)	73, 380, 969
BfaI	CTAG	1 cut(s)	1482
BfmI	CTRYAG	3 cut(s)	910, 954, 1440
BfrI	CTTAAG	1 cut(s)	128
BfuAI	ACCTGC	2 cut(s)	349, 1558
BglII	AGATCT	2 cut(s)	914, 1751
BisI	GCNGC	9 cut(s)	25, 109, 197, 356, 834, 1698, 1701, 1704, 1707
BlsI	GCNGC	9 cut(s)	26, 110, 198, 357, 835, 1699, 1702, 1705, 1708
BmcAI	AGTACT	1 cut(s)	1646
Bme1390I	CCNGG	2 cut(s)	380, 846
BmgT120I	GGNCC	1 cut(s)	860
BmiI	GGNNCC	2 cut(s)	1338, 1770
BmrFI	CCNGG	2 cut(s)	380, 846
BmrI	ACTGGG	1 cut(s)	234
BmsI	GCATC	4 cut(s)	373, 820, 1111, 1227
BmuI	ACTGGG	1 cut(s)	234
BpiI	GAAGAC	2 cut(s)	1557, 1671
BpmI	CTGGAG	2 cut(s)	362, 1308
Bpu10I	CCTNAGC	1 cut(s)	591
Bpu14I	TTCGAA	1 cut(s)	1662
Bsa29I	ATCGAT	1 cut(s)	1350
BsaAI	YACGTR	1 cut(s)	93
BsaBI	GATNNNNATC	1 cut(s)	1505
BsaHI	GRCGYC	1 cut(s)	1149
BsaJI	CCNNGG	4 cut(s)	449, 730, 844, 1018
BsaWI	WCCGGW	2 cut(s)	220, 1828
Bsc4I	CCNNNNNNNGG	5 cut(s)	289, 450, 734, 1019, 1516
Bse1I	ACTGG	4 cut(s)	115, 165, 240, 1098
Bse8I	GATNNNNATC	1 cut(s)	1505
BseBI	CCWGG	2 cut(s)	380, 846
BseCI	ATCGAT	1 cut(s)	1350
BseDI	CCNNGG	4 cut(s)	449, 730, 844, 1018
BseGI	GGATG	4 cut(s)	388, 577, 982, 1742
BseJI	GATNNNNATC	1 cut(s)	1505
BseLI	CCNNNNNNNGG	5 cut(s)	289, 450, 734, 1019, 1516
BseMII	CTCAG	2 cut(s)	560, 1419
BseNI	ACTGG	4 cut(s)	115, 165, 240, 1098
BseRI	GAGGAG	3 cut(s)	52, 750, 1439
BseXI	GCAGC	8 cut(s)	11, 120, 367, 845, 1687, 1690, 1693, 1709
BseYI	CCCAGC	1 cut(s)	814
BsgI	GTGCAG	1 cut(s)	403
Bsh1236I	CGCG	3 cut(s)	431, 772, 1778
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	5 cut(s)	5, 141, 602, 861, 926
BshVI	ATCGAT	1 cut(s)	1350
BsiEI	CGRYCG	1 cut(s)	15
BsiSI	CCGG	3 cut(s)	105, 221, 1829
BslFI	GGGAC	3 cut(s)	265, 1160, 1805
BslI	CCNNNNNNNGG	5 cut(s)	289, 450, 734, 1019, 1516
BsmAI	GTCTC	3 cut(s)	73, 380, 969
BsmBI	CGTCTC	1 cut(s)	73
BsmFI	GGGAC	3 cut(s)	265, 1160, 1805
BsmI	GAATGC	5 cut(s)	819, 866, 1223, 1719, 1737
BsnI	GGCC	5 cut(s)	5, 141, 602, 861, 926
Bsp119I	TTCGAA	1 cut(s)	1662
Bsp1286I	GDGCHC	1 cut(s)	1773
Bsp143I	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
Bsp19I	CCATGG	1 cut(s)	449
BspACI	CCGC	9 cut(s)	11, 35, 58, 197, 204, 252, 429, 1228, 1778
BspANI	GGCC	5 cut(s)	5, 141, 602, 861, 926
BspCNI	CTCAG	2 cut(s)	559, 1418
BspDI	ATCGAT	1 cut(s)	1350
BspFNI	CGCG	3 cut(s)	431, 772, 1778
BspLI	GGNNCC	2 cut(s)	1338, 1770
BspMI	ACCTGC	2 cut(s)	349, 1558
BspT104I	TTCGAA	1 cut(s)	1662
BspTI	CTTAAG	1 cut(s)	128
BsrBI	CCGCTC	1 cut(s)	60
BsrI	ACTGG	4 cut(s)	115, 165, 240, 1098
BssECI	CCNNGG	4 cut(s)	449, 730, 844, 1018
BssMI	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
BssNI	GRCGYC	1 cut(s)	1149
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	3 cut(s)	449, 730, 1018
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	2 cut(s)	380, 846
Bst4CI	ACNGT	6 cut(s)	49, 530, 715, 958, 1681, 1826
Bst6I	CTCTTC	5 cut(s)	554, 559, 915, 1096, 1416
BstACI	GRCGYC	1 cut(s)	1149
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	2 cut(s)	24, 1703
BstBAI	YACGTR	1 cut(s)	93
BstBI	TTCGAA	1 cut(s)	1662
BstC8I	GCNNGC	5 cut(s)	182, 689, 1063, 1255, 1468
BstDEI	CTNAG	6 cut(s)	546, 591, 756, 1405, 1522, 1648
BstDSI	CCRYGG	1 cut(s)	449
BstEII	GGTNACC	1 cut(s)	37
BstENI	CCTNNNNNAGG	1 cut(s)	1514
BstF5I	GGATG	4 cut(s)	388, 577, 982, 1742
BstFNI	CGCG	3 cut(s)	431, 772, 1778
BstHHI	GCGC	2 cut(s)	433, 1218
BstKTI	GATC	7 cut(s)	346, 877, 917, 1208, 1754, 1762, 1810
BstMAI	GTCTC	3 cut(s)	73, 380, 969
BstMBI	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
BstMCI	CGRYCG	1 cut(s)	15
BstMWI	GCNNNNNNNGC	5 cut(s)	24, 299, 1058, 1703, 1706
BstNI	CCWGG	2 cut(s)	380, 846
BstNSI	RCATGY	1 cut(s)	1065
BstPI	GGTNACC	1 cut(s)	37
BstSCI	CCNGG	2 cut(s)	378, 844
BstSFI	CTRYAG	3 cut(s)	910, 954, 1440
BstUI	CGCG	3 cut(s)	431, 772, 1778
BstV1I	GCAGC	8 cut(s)	11, 120, 367, 845, 1687, 1690, 1693, 1709
BstV2I	GAAGAC	2 cut(s)	1557, 1671
BstX2I	RGATCY	2 cut(s)	914, 1751
BstXI	CCANNNNNNTGG	1 cut(s)	586
BstYI	RGATCY	2 cut(s)	914, 1751
Bsu15I	ATCGAT	1 cut(s)	1350
BsuRI	GGCC	5 cut(s)	5, 141, 602, 861, 926
BsuTUI	ATCGAT	1 cut(s)	1350
BtgI	CCRYGG	1 cut(s)	449
BtgZI	GCGATG	1 cut(s)	1204
BtsCI	GGATG	4 cut(s)	388, 577, 982, 1742
BtsI	GCAGTG	2 cut(s)	490, 714
BtsIMutI	CAGTG	4 cut(s)	490, 526, 714, 1412
BveI	ACCTGC	2 cut(s)	349, 1558
Cac8I	GCNNGC	5 cut(s)	182, 689, 1063, 1255, 1468
CaiI	CAGNNNCTG	2 cut(s)	713, 1703
CfoI	GCGC	2 cut(s)	433, 1218
Cfr13I	GGNCC	1 cut(s)	860
ClaI	ATCGAT	1 cut(s)	1350
Csp6I	GTAC	6 cut(s)	118, 295, 477, 1091, 1294, 1645
CspCI	CAANNNNNGTGG	2 cut(s)	660, 695
CviAII	CATG	7 cut(s)	450, 797, 1062, 1175, 1297, 1555, 1632
CviQI	GTAC	6 cut(s)	118, 295, 477, 1091, 1294, 1645
DdeI	CTNAG	6 cut(s)	546, 591, 756, 1405, 1522, 1648
DpnI	GATC	7 cut(s)	345, 876, 916, 1207, 1753, 1761, 1809
DpnII	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
EaeI	YGGCCR	3 cut(s)	3, 139, 600
Eam1104I	CTCTTC	5 cut(s)	554, 559, 915, 1096, 1416
EarI	CTCTTC	5 cut(s)	554, 559, 915, 1096, 1416
Eco130I	CCWWGG	3 cut(s)	449, 730, 1018
Eco24I	GRGCYC	1 cut(s)	1773
Eco32I	GATATC	2 cut(s)	725, 1816
Eco57I	CTGAAG	2 cut(s)	683, 939
Eco72I	CACGTG	1 cut(s)	93
Eco91I	GGTNACC	1 cut(s)	37
EcoNI	CCTNNNNNAGG	1 cut(s)	1514
EcoO109I	RGGNCCY	1 cut(s)	860
EcoO65I	GGTNACC	1 cut(s)	37
EcoRI	GAATTC	2 cut(s)	650, 1658
EcoRII	CCWGG	2 cut(s)	378, 844
EcoRV	GATATC	2 cut(s)	725, 1816
EcoT14I	CCWWGG	3 cut(s)	449, 730, 1018
EcoT38I	GRGCYC	1 cut(s)	1773
ErhI	CCWWGG	3 cut(s)	449, 730, 1018
Esp3I	CGTCTC	1 cut(s)	73
FaeI	CATG	7 cut(s)	453, 800, 1065, 1178, 1300, 1558, 1635
FaqI	GGGAC	3 cut(s)	265, 1160, 1805
FatI	CATG	7 cut(s)	449, 796, 1061, 1174, 1296, 1554, 1631
FauNDI	CATATG	1 cut(s)	790
FbaI	TGATCA	3 cut(s)	343, 874, 1807
FblI	GTMKAC	2 cut(s)	909, 1626
Fnu4HI	GCNGC	9 cut(s)	25, 109, 197, 356, 834, 1698, 1701, 1704, 1707
FokI	GGATG	4 cut(s)	395, 584, 989, 1729
FriOI	GRGCYC	1 cut(s)	1773
Fsp4HI	GCNGC	9 cut(s)	25, 109, 197, 356, 834, 1698, 1701, 1704, 1707
FspBI	CTAG	1 cut(s)	1482
GlaI	GCGC	2 cut(s)	432, 1217
GluI	GCNGC	9 cut(s)	25, 109, 197, 356, 834, 1698, 1701, 1704, 1707
GsaI	CCCAGC	1 cut(s)	818
GsuI	CTGGAG	2 cut(s)	362, 1308
HaeIII	GGCC	5 cut(s)	5, 141, 602, 861, 926
HapII	CCGG	3 cut(s)	105, 221, 1829
HhaI	GCGC	2 cut(s)	433, 1218
Hin1I	GRCGYC	1 cut(s)	1149
Hin1II	CATG	7 cut(s)	453, 800, 1065, 1178, 1300, 1558, 1635
Hin6I	GCGC	2 cut(s)	431, 1216
HinP1I	GCGC	2 cut(s)	431, 1216
HindIII	AAGCTT	2 cut(s)	685, 1159
HinfI	GANTC	8 cut(s)	332, 1045, 1076, 1436, 1485, 1587, 1628, 1781
HpaII	CCGG	3 cut(s)	105, 221, 1829
HphI	GGTGA	3 cut(s)	31, 512, 1832
Hpy166II	GTNNAC	6 cut(s)	415, 910, 1091, 1395, 1537, 1627
Hpy188I	TCNGA	8 cut(s)	308, 348, 808, 919, 1120, 1408, 1747, 1759
Hpy188III	TCNNGA	5 cut(s)	171, 514, 655, 701, 1811
Hpy8I	GTNNAC	6 cut(s)	415, 910, 1091, 1395, 1537, 1627
Hpy99I	CGWCG	4 cut(s)	140, 193, 196, 1262
HpyAV	CCTTC	7 cut(s)	224, 265, 322, 455, 1181, 1374, 1456
HpyCH4III	ACNGT	6 cut(s)	49, 530, 715, 958, 1681, 1826
HpyCH4IV	ACGT	3 cut(s)	92, 1149, 1260
HpyF10VI	GCNNNNNNNGC	5 cut(s)	24, 299, 1058, 1703, 1706
HpyF3I	CTNAG	6 cut(s)	546, 591, 756, 1405, 1522, 1648
HpySE526I	ACGT	3 cut(s)	92, 1149, 1260
Hsp92I	GRCGYC	1 cut(s)	1149
Hsp92II	CATG	7 cut(s)	453, 800, 1065, 1178, 1300, 1558, 1635
HspAI	GCGC	2 cut(s)	431, 1216
Ksp22I	TGATCA	3 cut(s)	343, 874, 1807
Kzo9I	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
LmnI	GCTCC	3 cut(s)	65, 662, 1768
Lsp1109I	GCAGC	8 cut(s)	11, 120, 367, 845, 1687, 1690, 1693, 1709
LweI	GCATC	4 cut(s)	373, 820, 1111, 1227
MaeI	CTAG	1 cut(s)	1482
MaeII	ACGT	3 cut(s)	92, 1149, 1260
MaeIII	GTNAC	8 cut(s)	37, 500, 709, 751, 781, 1029, 1430, 1820
MalI	GATC	7 cut(s)	345, 876, 916, 1207, 1753, 1761, 1809
MbiI	CCGCTC	1 cut(s)	60
MboI	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
MfeI	CAATTG	1 cut(s)	677
MflI	RGATCY	2 cut(s)	914, 1751
MhlI	GDGCHC	1 cut(s)	1773
MlsI	TGGCCA	1 cut(s)	602
MluNI	TGGCCA	1 cut(s)	602
MlyI	GAGTC	4 cut(s)	341, 1085, 1430, 1622
MmeI	TCCRAC	4 cut(s)	100, 1020, 1665, 1746
Mox20I	TGGCCA	1 cut(s)	602
MroXI	GAANNNNTTC	1 cut(s)	234
MscI	TGGCCA	1 cut(s)	602
MseI	TTAA	7 cut(s)	51, 129, 1055, 1136, 1305, 1449, 1580
MslI	CAYNNNNRTG	3 cut(s)	893, 1350, 1679
Msp20I	TGGCCA	1 cut(s)	602
MspA1I	CMGCKG	2 cut(s)	206, 1700
MspCI	CTTAAG	1 cut(s)	128
MspI	CCGG	3 cut(s)	105, 221, 1829
MspR9I	CCNGG	2 cut(s)	380, 846
MunI	CAATTG	1 cut(s)	677
Mva1269I	GAATGC	5 cut(s)	819, 866, 1223, 1719, 1737
MvaI	CCWGG	2 cut(s)	380, 846
MvnI	CGCG	3 cut(s)	431, 772, 1778
MwoI	GCNNNNNNNGC	5 cut(s)	24, 299, 1058, 1703, 1706
NcoI	CCATGG	1 cut(s)	449
NdeI	CATATG	1 cut(s)	790
NdeII	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
NlaIII	CATG	7 cut(s)	453, 800, 1065, 1178, 1300, 1558, 1635
NlaIV	GGNNCC	2 cut(s)	1338, 1770
NmeAIII	GCCGAG	1 cut(s)	952
NmuCI	GTSAC	5 cut(s)	37, 500, 709, 1430, 1820
NspI	RCATGY	1 cut(s)	1065
NspV	TTCGAA	1 cut(s)	1662
PaeI	GCATGC	1 cut(s)	1065
PcsI	WCGNNNNNNNCGW	2 cut(s)	188, 197
PctI	GAATGC	5 cut(s)	819, 866, 1223, 1719, 1737
PdmI	GAANNNNTTC	1 cut(s)	234
PfeI	GAWTC	4 cut(s)	1045, 1485, 1587, 1781
PflMI	CCANNNNNTGG	1 cut(s)	289
PfoI	TCCNGGA	1 cut(s)	378
PkrI	GCNGC	9 cut(s)	26, 110, 198, 357, 835, 1699, 1702, 1705, 1708
PleI	GAGTC	4 cut(s)	340, 1084, 1430, 1622
PmaCI	CACGTG	1 cut(s)	93
PmlI	CACGTG	1 cut(s)	93
PpsI	GAGTC	4 cut(s)	340, 1084, 1430, 1622
Ppu21I	YACGTR	1 cut(s)	93
Psp6I	CCWGG	2 cut(s)	378, 844
PspCI	CACGTG	1 cut(s)	93
PspEI	GGTNACC	1 cut(s)	37
PspFI	CCCAGC	1 cut(s)	814
PspGI	CCWGG	2 cut(s)	378, 844
PspN4I	GGNNCC	2 cut(s)	1338, 1770
PspPI	GGNCC	1 cut(s)	860
PsrI	GAACNNNNNNTAC	2 cut(s)	287, 319
PstNI	CAGNNNCTG	2 cut(s)	713, 1703
PsuI	RGATCY	2 cut(s)	914, 1751
PvuII	CAGCTG	1 cut(s)	1700
RsaI	GTAC	6 cut(s)	119, 296, 478, 1092, 1295, 1646
RsaNI	GTAC	6 cut(s)	118, 295, 477, 1091, 1294, 1645
RseI	CAYNNNNRTG	3 cut(s)	893, 1350, 1679
SaqAI	TTAA	7 cut(s)	51, 129, 1055, 1136, 1305, 1449, 1580
SatI	GCNGC	9 cut(s)	25, 109, 197, 356, 834, 1698, 1701, 1704, 1707
Sau3AI	GATC	7 cut(s)	343, 874, 914, 1205, 1751, 1759, 1807
Sau96I	GGNCC	1 cut(s)	860
ScaI	AGTACT	1 cut(s)	1646
SchI	GAGTC	4 cut(s)	341, 1085, 1430, 1622
ScrFI	CCNGG	2 cut(s)	380, 846
SduI	GDGCHC	1 cut(s)	1773
SfaNI	GCATC	4 cut(s)	373, 820, 1111, 1227
SfcI	CTRYAG	3 cut(s)	910, 954, 1440
SfuI	TTCGAA	1 cut(s)	1662
SmiMI	CAYNNNNRTG	3 cut(s)	893, 1350, 1679
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
SphI	GCATGC	1 cut(s)	1065
SsiI	CCGC	9 cut(s)	11, 35, 58, 197, 204, 252, 429, 1228, 1778
SspI	AATATT	1 cut(s)	934
SspMI	CTAG	1 cut(s)	1482
StyD4I	CCNGG	2 cut(s)	378, 844
StyI	CCWWGG	3 cut(s)	449, 730, 1018
TaaI	ACNGT	6 cut(s)	49, 530, 715, 958, 1681, 1826
TaiI	ACGT	3 cut(s)	95, 1152, 1263
TaqI	TCGA	5 cut(s)	170, 1204, 1350, 1662, 1854
TatI	WGTACW	4 cut(s)	476, 1090, 1293, 1644
TauI	GCSGC	1 cut(s)	199
TfiI	GAWTC	4 cut(s)	1045, 1485, 1587, 1781
Tru1I	TTAA	7 cut(s)	51, 129, 1055, 1136, 1305, 1449, 1580
Tru9I	TTAA	7 cut(s)	51, 129, 1055, 1136, 1305, 1449, 1580
TscAI	CASTG	4 cut(s)	490, 533, 714, 1419
TseFI	GTSAC	5 cut(s)	37, 500, 709, 1430, 1820
TseI	GCWGC	8 cut(s)	24, 108, 355, 833, 1697, 1700, 1703, 1706
Tsp45I	GTSAC	5 cut(s)	37, 500, 709, 1430, 1820
TspDTI	ATGAA	6 cut(s)	1163, 1313, 1334, 1545, 1563, 1595
TspGWI	ACGGA	5 cut(s)	55, 61, 325, 1599, 1612
TspRI	CASTG	4 cut(s)	490, 533, 714, 1419
Van91I	CCANNNNNTGG	1 cut(s)	289
Vha464I	CTTAAG	1 cut(s)	128
XagI	CCTNNNNNAGG	1 cut(s)	1514
XapI	RAATTY	5 cut(s)	581, 650, 1247, 1658, 1837
XceI	RCATGY	1 cut(s)	1065
XmiI	GTMKAC	2 cut(s)	909, 1626
XmnI	GAANNNNTTC	1 cut(s)	234
XspI	CTAG	1 cut(s)	1482
ZraI	GACGTC	1 cut(s)	1150
ZrmI	AGTACT	1 cut(s)	1646

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.