Rroxscaffold_7G00174930

Protease Do-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0007826
Physical Location & Seq
Reverse (-)
14626879 .. 14633707
6829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00174930.1

Sequence Viewer

Length: 1461 bp
ATGGCCGTTGCGGTCGTTAACTGCTACTTCTCCGTTGTCACCTCCACCGTTAAATTCCGTTCCGCCGTTTCCGCCGCCCAACCTTGCTTCTCCGCGTCGCTGTGGTCAAGTAGTCGCTGCCACACTCTTAAACCGGTTTGCCGCAGTAACAGAGCTTCGAATTCACGGAAAGGCGATAAATCACCGTCGGAGAAAGAAGGTGTTGCTAAGAAGCTTATTGGAAATGGTGACATTAGGAGTGGTAGTGGTAGAGGGAAGAAGGGGCAGGCCCAGCCTGCGGCTTATAAGCCATTCGGATCGCAGAGAAAAGAGAAGAAAGAGTCCGTAGTTGATCTAAAGGAGAAGCAGGTTGAAGCGAGAAATCTTCAGGATGCTGATTTTCTCAATGCCGTCGTTAAGGTTAAAGTGAAGAGAAGGGGAGATGATACCAAATACGTGGCTAAGGTATTTGATCCTCATAAAATTAACATTGTGTTTCTCAATATCTTGGAGATAATTAAGATGCCACAGCCTATTATCAGTAGAGGGGATTGTGATGTTTTGGCCAAAGGTGTTGATTGTGATATAGCCTTACTTACAGTAGAAAGTGAGGAATTTTGGAAAGGAGCAGAGCCACTCCATTTCGGAAGCTTGCCACATCTTCAGGAAGCAGTGACTGTTGTAGGATATCCCCTTGGAGGAGATACCATTTCAGTAACGAAGGGGGTTGTATCGCGTATAGAGGTCTACAGATCTGAAGAGGCCGAGAATATTGGATATGTGATTCCTACTACAGTTGTCTCTCACTTTTTGAATGATTATGAGAGGAACGGGAAGTACACAGGTTTCCCTTGCCTTGGTGTTATGTTGCAAAAGTTGGAGAATCCTGCATTACGAGCATGCTTAAAAGTGGAGTCTATTGAGGGTGTACTGGTGCGAAGAGTAGAACCCACTTGTGATGCACATAATGTACTAAAGGAGGGTGACGTGATTGTAAGCTTCGATGATGTGCATGTAGGGTGTGAAGGAACAGTACCATTTCGATCAAACGAGCGCATTGCATTCCGCTACCTTATTAGCCAGAAATTTGCAGGTGATGTTGCAGATCTTGGTATTATTAGAGCCGGAGAATTCATGAAAGTCAAAGCAGAGTTGAACCCACGAGTGCATTTGTTGAAACTTTTGGCAAAGGCACGCTACTCTTTGGCTAGATTCAAAGGAGAACAGATTGTAATCCTTTCACAGCACAAGTACTTGGTCTTTGAATTTGAAGACAACTACATAACAGTTCTAGAAAGAGAAGCTGCACTAGCTTCTTCAACCAGCATTCTCAAAGATTACGGGATTCCAGCTGAAAGATCTTCAGATCTGTTGGAGCCCTACATAGATTCAGTTGTGGACGGCCAAGCTGACCAGGAGGATTTGGGTGACAGTCCGGTTTCAAATCTGGAAATTGGCTTTGATGGACTTATTTGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

486

Amino Acids

53.59

Weight (kDa)

7.1

Isoelectric Point (pI)

42.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDZ_3 PF17815 408 - 449 7.4e-06 PDZ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 285
AarI CACCTGC 1 cut(s) 1061
Acc36I ACCTGC 2 cut(s) 337, 1061
AccI GTMKAC 1 cut(s) 726
AccII CGCG 2 cut(s) 95, 715
AciI CCGC 8 cut(s) 11, 63, 72, 75, 93, 142, 278, 1045
AclWI GGATC 2 cut(s) 304, 446
AcoI YGGCCR 3 cut(s) 3, 543, 1381
AcsI RAATTY 6 cut(s) 53, 160, 593, 1064, 1109, 1244
AcuI CTGAAG 4 cut(s) 350, 626, 756, 1326
AfaI GTAC 5 cut(s) 818, 909, 951, 1014, 1232
AfiI CCNNNNNNNGG 3 cut(s) 277, 677, 836
AgeI ACCGGT 1 cut(s) 133
AgsI TTSAA 9 cut(s) 353, 793, 1135, 1156, 1195, 1244, 1250, 1299, 1422
AjiI CACGTC 1 cut(s) 967
AjnI CCWGG 1 cut(s) 1392
AluBI AGCT 8 cut(s) 155, 214, 630, 978, 1283, 1292, 1331, 1388
AluI AGCT 8 cut(s) 155, 214, 630, 978, 1283, 1292, 1331, 1388
Alw26I GTCTC 1 cut(s) 784
AlwI GGATC 2 cut(s) 304, 446
AlwNI CAGNNNCTG 1 cut(s) 656
AoxI GGCC 5 cut(s) 3, 267, 543, 741, 1381
ApeKI GCWGC 2 cut(s) 117, 1283
ApoI RAATTY 6 cut(s) 53, 160, 593, 1064, 1109, 1244
AsiGI ACCGGT 1 cut(s) 133
AspLEI GCGC 1 cut(s) 1035
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 6 cut(s) 31, 174, 239, 974, 1085, 1418
AsuII TTCGAA 1 cut(s) 158
BalI TGGCCA 1 cut(s) 545
BanII GRGCYC 1 cut(s) 1359
BarI GAAGNNNNNNTAC 4 cut(s) 139, 171, 996, 1028
BauI CACGAG 1 cut(s) 1140
BbsI GAAGAC 1 cut(s) 1257
BbvI GCAGC 2 cut(s) 104, 1270
BccI CCATC 1 cut(s) 1436
BceAI ACGGC 3 cut(s) 50, 374, 1396
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 1 cut(s) 1394
BcoDI GTCTC 1 cut(s) 784
BfaI CTAG 3 cut(s) 1188, 1271, 1289
BfmI CTRYAG 2 cut(s) 727, 771
BfuAI ACCTGC 2 cut(s) 337, 1061
BglII AGATCT 4 cut(s) 731, 1084, 1337, 1345
BisI GCNGC 5 cut(s) 75, 118, 142, 279, 1284
BlsI GCNGC 5 cut(s) 76, 119, 143, 280, 1285
BmcAI AGTACT 1 cut(s) 1232
Bme1390I CCNGG 1 cut(s) 1394
BmgBI CACGTC 1 cut(s) 967
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 1 cut(s) 1356
BmrFI CCNGG 1 cut(s) 1394
BmsI GCATC 3 cut(s) 361, 492, 928
BpiI GAAGAC 1 cut(s) 1257
Bpu10I CCTNAGC 1 cut(s) 441
Bpu14I TTCGAA 1 cut(s) 158
BsaAI YACGTR 1 cut(s) 436
BsaBI GATNNNNATC 1 cut(s) 1211
BsaJI CCNNGG 2 cut(s) 673, 835
BsaWI WCCGGW 2 cut(s) 133, 1414
BsaXI ACNNNNNCTCC 2 cut(s) 229, 259
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 677, 836
Bse118I RCCGGY 1 cut(s) 133
Bse1I ACTGG 1 cut(s) 915
Bse3DI GCAATG 1 cut(s) 1035
Bse8I GATNNNNATC 1 cut(s) 1211
BseBI CCWGG 1 cut(s) 1394
BseDI CCNNGG 2 cut(s) 673, 835
BseGI GGATG 1 cut(s) 376
BseJI GATNNNNATC 1 cut(s) 1211
BseLI CCNNNNNNNGG 3 cut(s) 277, 677, 836
BseMI GCAATG 1 cut(s) 1035
BseNI ACTGG 1 cut(s) 915
BseRI GAGGAG 1 cut(s) 693
BseXI GCAGC 2 cut(s) 104, 1270
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 1 cut(s) 1269
Bsh1236I CGCG 2 cut(s) 95, 715
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 5 cut(s) 5, 269, 545, 743, 1383
BshTI ACCGGT 1 cut(s) 133
BsiEI CGRYCG 1 cut(s) 15
BsiSI CCGG 3 cut(s) 134, 1104, 1415
BslI CCNNNNNNNGG 3 cut(s) 277, 677, 836
BsmAI GTCTC 1 cut(s) 784
BsmI GAATGC 2 cut(s) 1040, 1305
BsnI GGCC 5 cut(s) 5, 269, 545, 743, 1383
Bsp119I TTCGAA 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 1359
Bsp143I GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
BspACI CCGC 8 cut(s) 11, 63, 72, 75, 93, 142, 278, 1045
BspANI GGCC 5 cut(s) 5, 269, 545, 743, 1383
BspFNI CGCG 2 cut(s) 95, 715
BspHI TCATGA 1 cut(s) 1113
BspLI GGNNCC 1 cut(s) 1356
BspMI ACCTGC 2 cut(s) 337, 1061
BspPI GGATC 2 cut(s) 304, 446
BspT104I TTCGAA 1 cut(s) 158
BsrDI GCAATG 1 cut(s) 1035
BsrFI RCCGGY 1 cut(s) 133
BsrI ACTGG 1 cut(s) 915
BssAI RCCGGY 1 cut(s) 133
BssECI CCNNGG 2 cut(s) 673, 835
BssMI GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
BssSI CACGAG 1 cut(s) 1140
BssT1I CCWWGG 2 cut(s) 673, 835
Bst2BI CACGAG 1 cut(s) 1140
Bst2UI CCWGG 1 cut(s) 1394
Bst4CI ACNGT 8 cut(s) 49, 186, 580, 658, 775, 1012, 1267, 1412
Bst6I CTCTTC 3 cut(s) 404, 732, 913
BstBAI YACGTR 1 cut(s) 436
BstBI TTCGAA 1 cut(s) 158
BstC8I GCNNGC 5 cut(s) 267, 276, 632, 880, 1174
BstDEI CTNAG 2 cut(s) 207, 441
BstF5I GGATG 1 cut(s) 376
BstFNI CGCG 2 cut(s) 95, 715
BstHHI GCGC 1 cut(s) 1035
BstKTI GATC 8 cut(s) 299, 334, 454, 734, 1025, 1087, 1340, 1348
BstMAI GTCTC 1 cut(s) 784
BstMBI GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 5 cut(s) 71, 271, 275, 875, 1289
BstNI CCWGG 1 cut(s) 1394
BstNSI RCATGY 2 cut(s) 882, 995
BstSCI CCNGG 1 cut(s) 1392
BstSFI CTRYAG 2 cut(s) 727, 771
BstUI CGCG 2 cut(s) 95, 715
BstV1I GCAGC 2 cut(s) 104, 1270
BstV2I GAAGAC 1 cut(s) 1257
BstX2I RGATCY 4 cut(s) 731, 1084, 1337, 1345
BstXI CCANNNNNNTGG 1 cut(s) 436
BstYI RGATCY 4 cut(s) 731, 1084, 1337, 1345
BsuRI GGCC 5 cut(s) 5, 269, 545, 743, 1383
BtrI CACGTC 1 cut(s) 967
BtsCI GGATG 1 cut(s) 376
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 1 cut(s) 657
BveI ACCTGC 2 cut(s) 337, 1061
Cac8I GCNNGC 5 cut(s) 267, 276, 632, 880, 1174
CaiI CAGNNNCTG 1 cut(s) 656
CciI TCATGA 1 cut(s) 1113
CfoI GCGC 1 cut(s) 1035
Cfr10I RCCGGY 1 cut(s) 133
Cfr13I GGNCC 1 cut(s) 268
CseI GACGC 1 cut(s) 84
Csp6I GTAC 5 cut(s) 817, 908, 950, 1013, 1231
CspAI ACCGGT 1 cut(s) 133
CviAII CATG 3 cut(s) 879, 992, 1114
CviQI GTAC 5 cut(s) 817, 908, 950, 1013, 1231
DdeI CTNAG 2 cut(s) 207, 441
DpnI GATC 8 cut(s) 298, 333, 453, 733, 1024, 1086, 1339, 1347
DpnII GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
EaeI YGGCCR 3 cut(s) 3, 543, 1381
Eam1104I CTCTTC 3 cut(s) 404, 732, 913
EarI CTCTTC 3 cut(s) 404, 732, 913
EciI GGCGGA 2 cut(s) 52, 61
Eco130I CCWWGG 2 cut(s) 673, 835
Eco24I GRGCYC 1 cut(s) 1359
Eco32I GATATC 1 cut(s) 668
Eco57I CTGAAG 4 cut(s) 350, 626, 756, 1326
EcoRI GAATTC 2 cut(s) 160, 1109
EcoRII CCWGG 1 cut(s) 1392
EcoRV GATATC 1 cut(s) 668
EcoT14I CCWWGG 2 cut(s) 673, 835
EcoT38I GRGCYC 1 cut(s) 1359
ErhI CCWWGG 2 cut(s) 673, 835
FaeI CATG 3 cut(s) 882, 995, 1117
FatI CATG 3 cut(s) 878, 991, 1113
FblI GTMKAC 1 cut(s) 726
Fnu4HI GCNGC 5 cut(s) 75, 118, 142, 279, 1284
FokI GGATG 1 cut(s) 383
FriOI GRGCYC 1 cut(s) 1359
Fsp4HI GCNGC 5 cut(s) 75, 118, 142, 279, 1284
FspBI CTAG 3 cut(s) 1188, 1271, 1289
GlaI GCGC 1 cut(s) 1034
GluI GCNGC 5 cut(s) 75, 118, 142, 279, 1284
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 5 cut(s) 5, 269, 545, 743, 1383
HapII CCGG 3 cut(s) 134, 1104, 1415
HgaI GACGC 1 cut(s) 84
HhaI GCGC 1 cut(s) 1035
Hin1II CATG 3 cut(s) 882, 995, 1117
Hin6I GCGC 1 cut(s) 1033
HinP1I GCGC 1 cut(s) 1033
HincII GTYRAC 1 cut(s) 19
HindII GTYRAC 1 cut(s) 19
HindIII AAGCTT 3 cut(s) 212, 628, 976
HinfI GANTC 7 cut(s) 320, 763, 862, 893, 1191, 1324, 1367
HpaI GTTAAC 1 cut(s) 19
HpaII CCGG 3 cut(s) 134, 1104, 1415
HphI GGTGA 6 cut(s) 31, 174, 239, 974, 1085, 1418
Hpy166II GTNNAC 5 cut(s) 19, 727, 819, 908, 1378
Hpy188I TCNGA 5 cut(s) 190, 296, 626, 736, 1345
Hpy188III TCNNGA 5 cut(s) 368, 644, 1114, 1271, 1427
Hpy8I GTNNAC 5 cut(s) 19, 727, 819, 908, 1378
Hpy99I CGWCG 3 cut(s) 100, 190, 395
HpyAV CCTTC 5 cut(s) 191, 253, 408, 694, 998
HpyCH4III ACNGT 8 cut(s) 49, 186, 580, 658, 775, 1012, 1267, 1412
HpyCH4IV ACGT 2 cut(s) 435, 966
HpyCH4V TGCA 9 cut(s) 850, 869, 941, 991, 1040, 1070, 1082, 1147, 1286
HpyF10VI GCNNNNNNNGC 5 cut(s) 71, 271, 275, 875, 1289
HpyF3I CTNAG 2 cut(s) 207, 441
HpySE526I ACGT 2 cut(s) 435, 966
Hsp92II CATG 3 cut(s) 882, 995, 1117
HspAI GCGC 1 cut(s) 1033
KspAI GTTAAC 1 cut(s) 19
Kzo9I GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
LmnI GCTCC 2 cut(s) 605, 1354
Lsp1109I GCAGC 2 cut(s) 104, 1270
LweI GCATC 3 cut(s) 361, 492, 928
MaeI CTAG 3 cut(s) 1188, 1271, 1289
MaeII ACGT 2 cut(s) 435, 966
MaeIII GTNAC 7 cut(s) 37, 146, 227, 652, 694, 962, 1406
MalI GATC 8 cut(s) 298, 333, 453, 733, 1024, 1086, 1339, 1347
MboI GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
MflI RGATCY 4 cut(s) 731, 1084, 1337, 1345
MhlI GDGCHC 1 cut(s) 1359
MlsI TGGCCA 1 cut(s) 545
MluCI AATT 9 cut(s) 53, 160, 462, 495, 593, 1064, 1109, 1244, 1431
MluNI TGGCCA 1 cut(s) 545
MlyI GAGTC 2 cut(s) 329, 902
MmeI TCCRAC 3 cut(s) 168, 837, 1332
Mox20I TGGCCA 1 cut(s) 545
MscI TGGCCA 1 cut(s) 545
MseI TTAA 9 cut(s) 18, 51, 129, 396, 402, 465, 498, 884, 1459
Msp20I TGGCCA 1 cut(s) 545
MspA1I CMGCKG 1 cut(s) 1331
MspI CCGG 3 cut(s) 134, 1104, 1415
MspR9I CCNGG 1 cut(s) 1394
Mva1269I GAATGC 2 cut(s) 1040, 1305
MvaI CCWGG 1 cut(s) 1394
MvnI CGCG 2 cut(s) 95, 715
MwoI GCNNNNNNNGC 5 cut(s) 71, 271, 275, 875, 1289
NdeII GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
NlaIII CATG 3 cut(s) 882, 995, 1117
NlaIV GGNNCC 1 cut(s) 1356
NmeAIII GCCGAG 1 cut(s) 769
NmuCI GTSAC 5 cut(s) 37, 227, 652, 962, 1406
NspI RCATGY 2 cut(s) 882, 995
NspV TTCGAA 1 cut(s) 158
PaeI GCATGC 1 cut(s) 882
PagI TCATGA 1 cut(s) 1113
PaqCI CACCTGC 1 cut(s) 1061
PctI GAATGC 2 cut(s) 1040, 1305
PfeI GAWTC 5 cut(s) 763, 862, 1191, 1324, 1367
PinAI ACCGGT 1 cut(s) 133
PkrI GCNGC 5 cut(s) 76, 119, 143, 280, 1285
PleI GAGTC 2 cut(s) 328, 901
PpsI GAGTC 2 cut(s) 328, 901
Ppu21I YACGTR 1 cut(s) 436
PsiI TTATAA 1 cut(s) 285
Psp6I CCWGG 1 cut(s) 1392
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 1392
PspN4I GGNNCC 1 cut(s) 1356
PspPI GGNCC 1 cut(s) 268
PsrI GAACNNNNNNTAC 2 cut(s) 800, 832
PstNI CAGNNNCTG 1 cut(s) 656
PsuI RGATCY 4 cut(s) 731, 1084, 1337, 1345
PvuII CAGCTG 1 cut(s) 1331
RsaI GTAC 5 cut(s) 818, 909, 951, 1014, 1232
RsaNI GTAC 5 cut(s) 817, 908, 950, 1013, 1231
SaqAI TTAA 9 cut(s) 18, 51, 129, 396, 402, 465, 498, 884, 1459
SatI GCNGC 5 cut(s) 75, 118, 142, 279, 1284
Sau3AI GATC 8 cut(s) 296, 331, 451, 731, 1022, 1084, 1337, 1345
Sau96I GGNCC 1 cut(s) 268
ScaI AGTACT 1 cut(s) 1232
SchI GAGTC 2 cut(s) 329, 902
ScrFI CCNGG 1 cut(s) 1394
SduI GDGCHC 1 cut(s) 1359
SfaNI GCATC 3 cut(s) 361, 492, 928
SfcI CTRYAG 2 cut(s) 727, 771
SfuI TTCGAA 1 cut(s) 158
SphI GCATGC 1 cut(s) 882
Sse9I AATT 9 cut(s) 53, 160, 462, 495, 593, 1064, 1109, 1244, 1431
SsiI CCGC 8 cut(s) 11, 63, 72, 75, 93, 142, 278, 1045
SspI AATATT 1 cut(s) 751
SspMI CTAG 3 cut(s) 1188, 1271, 1289
StyD4I CCNGG 1 cut(s) 1392
StyI CCWWGG 2 cut(s) 673, 835
TaaI ACNGT 8 cut(s) 49, 186, 580, 658, 775, 1012, 1267, 1412
TaiI ACGT 2 cut(s) 438, 969
TaqI TCGA 3 cut(s) 158, 981, 1021
TasI AATT 9 cut(s) 53, 160, 462, 495, 593, 1064, 1109, 1244, 1431
TatI WGTACW 4 cut(s) 816, 907, 949, 1230
TauI GCSGC 3 cut(s) 77, 144, 281
TfiI GAWTC 5 cut(s) 763, 862, 1191, 1324, 1367
Tru1I TTAA 9 cut(s) 18, 51, 129, 396, 402, 465, 498, 884, 1459
Tru9I TTAA 9 cut(s) 18, 51, 129, 396, 402, 465, 498, 884, 1459
TscAI CASTG 1 cut(s) 657
TseFI GTSAC 5 cut(s) 37, 227, 652, 962, 1406
TseI GCWGC 2 cut(s) 117, 1283
Tsp45I GTSAC 5 cut(s) 37, 227, 652, 962, 1406
TspDTI ATGAA 2 cut(s) 1102, 1130
TspGWI ACGGA 4 cut(s) 22, 47, 181, 313
TspRI CASTG 1 cut(s) 657
XapI RAATTY 6 cut(s) 53, 160, 593, 1064, 1109, 1244
XbaI TCTAGA 1 cut(s) 1270
XceI RCATGY 2 cut(s) 882, 995
XmiI GTMKAC 1 cut(s) 726
XspI CTAG 3 cut(s) 1188, 1271, 1289
ZrmI AGTACT 1 cut(s) 1232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.