Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0007826

Physical Location & Seq

Genomic Coordinates Forward (+)

41506900 .. 41513389

Gene Structure 6490 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1298100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1875 bp

ATGGCCGTTGCGGTCGGCAACTGCTGCTTCTCTGCGCTCACCTCCACCGTTAAAATCCGCTCCTTCGTCTCCGTCCAACCCTTCTTCGCCACGTGGCTCTGTTCCGGCAGCTACCAGTGCCAAGCTCTTAAGCCCGTCAGTCGGAGCAGGAGAGAACGAGCTTCCAGTTCGAGAAAAGGCTCGCCGTCGTCGCCTCCGTCGTCCGCTGATAAATATCAACCGGAGGGAGAAGGCGTTCCCAATAAGTTTAGCGGAAATGGCAGAGGGAAGAAGGGGCAATCTCAATCGGTGGCGTACAGAGGGTTCGGAGCGCAACGAAAAGAGCGGAAGGAGTCTGTAGTTAATCAGAAAGAGCAGCAGGTTGAACCGAAAAGTCTCCAGGATGCCGATTTTCTCAATGCCGTTGTTAAGGTTTACTGCACCCATACCGCGCCCGACTACTCCCTTCCTTGGCAAAAACAAAGACAATATACAAGTACAGGCAGTGCTTTTATGATTGGTGACGGGAAACTCTTGACAAATGCCCACTGTGTTGAGCATTATACTCAGGTCAAAGTGAAGAGAAGAGGGGATGATACCAAATATGTGGCTAAGGTTTTGGCCAGAGGTGTTGATTGTGATATAGCCTTACTTTCAGTAGAAAGTGAGGAATTCTGGAAAGGAGCAGAACCACTCCAATTGGGAAGCTTGCCACATCTTCAGGAATCAGTGACTGTTGTAGGATATCCCCTTGGAGGGGATACCATTTCAGTAACTAAGGGGGTTGTATCGCGTATAGAGGTTACATCATATGCTCATGGGTCATCTGATTTGCTGGGCATTCAGATTGATGCAGCAATAAATCCTGGTAATAGTGGTGGCCCTGCATTCAATGATAAAGGGGAGTGCATTGGTGTGGCATTTCAGGTCTACAGATCTGAGGAGGCTGAGAATATTGGTTATGTCATTCCTACTACAGTTGTGTCTCATTTTTTGGATGACTATGAGAGGAACGGGAAGTACACAGGTTTCCCTTGCCTTGGTGTATTGTTGCAGAAGTTGGAGAATCCAGCATTAAAGGCGTGCCTGAAAGTAGAGTCTATTGAGGGTGTACTGGTCCGAAGAGTTGAACCCACTTCTGATGCACATAATGTATTAAAGGAGGGGGACGTCATTGTAAGCTTTGATGATGTTCATGTAGGGTGTGAAGGAACAGTACCCTTTCGATCAAATGAGCGCATCGCATTCCGCTACCTCATTAGCCAAAAATTTGCGGGCGATGTTGCAGATATTGGTATTATTAGAGCTGGAGAATATATGAAAGTTAAAGCAGTTTTGCACCCACGAGTGCATTTGGTTCCCTTTCATATCGACGGAGGTCAGCCTTCTTACTTAATAATTGCCGGTTTGGTGTTTACCCCCCTCTCAGAACCACTGATAGATGAGGAGTGTGAAGACTCTATAGGGCTTAAACTCTTGGCAAAGGCGCGCTACTCTTTGGCTAGATTCAAAGGGGAACAGATTGTAATCCTATCACAGGTCTTGGCAAATGAAGTAAACATTGGGTACGAAGACATGAGCAATCAGCAGGTTTTGAAGTTAAACGGAACTCAAATAAGGAACATTCATCATTTGGCCTACCTCGTAGATTCATGCAAGGACAAGTACTTAGTCTTCGAGTTTGAAGACAACTACATAACCGTTTTGGAAAGGGAAGCAGCTCTTGCTGCATCATCCTGCATTCTCAAAGACTATGGGATTCCATCCGAAAGATCTTCCGATCTGTCGGAGCCCTACGTGGATTCGCTTGGGGACAACCAAGCAGTTGATCAGGATATTGGCGACAGTCCAGTTTCAAATTTGGAAATCGGGTTCGATGGAATTATTTGGGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PDZ_2 Trypsin_2

Protein Analysis

625

Amino Acids

68.3

Weight (kDa)

5.66

Isoelectric Point (pI)

42.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Trypsin	PF00089	140 - 322	6.5e-25	Trypsin
Trypsin_2	PF13365	161 - 299	2.6e-26	Trypsin-like peptidase domain
PDZ_3	PF17815	446 - 587	2.8e-45	PDZ domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0007826)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G47940 AT2G47940
fragaria_vesca	FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740 FvH4_2g24740
malus_domestica	MD00G1056100.v1.1 MD11G1298100.v1.1
prunus_persica	Prupe.8G252400_v2.0.a1
pyrus_communis	pycom11g26180
rosa_chinensis	RchiOBHm_Chr6g0292561
rosa_laevigata	RLG00000012009
rosa_multiflora	Rmu_sc0000515.1_g000002
rosa_roxburghii	Rroxscaffold_7G00174930
rosa_rugosa	Rorug06G0228000 Rorug06G0228100
rosa_samantha	Rh6AG341200 Rh6BG348600 Rh6CG355500 Rh6DG341300
rosa_wichuraiana	Rw6G029720

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1152
Acc36I	ACCTGC	2 cut(s)	349, 1558
AccBSI	CCGCTC	2 cut(s)	60, 325
AccI	GTMKAC	1 cut(s)	909
AccII	CGCG	3 cut(s)	431, 772, 1468
AciI	CCGC	8 cut(s)	11, 58, 204, 252, 325, 429, 1228, 1253
AcoI	YGGCCR	2 cut(s)	3, 600
AcsI	RAATTY	3 cut(s)	650, 1247, 1837
AcuI	CTGAAG	1 cut(s)	683
AcvI	CACGTG	1 cut(s)	93
AcyI	GRCGYC	1 cut(s)	1149
AfaI	GTAC	7 cut(s)	296, 478, 1001, 1092, 1197, 1547, 1646
AfiI	CCNNNNNNNGG	7 cut(s)	141, 450, 734, 735, 736, 1019, 1516
AflII	CTTAAG	1 cut(s)	128
AgsI	TTSAA	7 cut(s)	365, 871, 1109, 1489, 1576, 1664, 1836
AjnI	CCWGG	2 cut(s)	378, 844
AjuI	GAANNNNNNNTTGG	2 cut(s)	1524, 1556
AloI	GAACNNNNNNTCC	4 cut(s)	219, 251, 1835, 1867
AluBI	AGCT	7 cut(s)	111, 125, 161, 687, 1161, 1286, 1700
AluI	AGCT	7 cut(s)	111, 125, 161, 687, 1161, 1286, 1700
Alw26I	GTCTC	3 cut(s)	73, 380, 969
AlwNI	CAGNNNCTG	1 cut(s)	713
AoxI	GGCC	4 cut(s)	3, 600, 859, 1614
ApeKI	GCWGC	6 cut(s)	24, 108, 355, 833, 1697, 1706
ApoI	RAATTY	3 cut(s)	650, 1247, 1837
Asp700I	GAANNNNTTC	1 cut(s)	234
AspLEI	GCGC	6 cut(s)	37, 313, 433, 1218, 1468, 1470
AspS9I	GGNCC	2 cut(s)	860, 1096
AsuHPI	GGTGA	2 cut(s)	31, 512
AvaII	GGWCC	1 cut(s)	1096
BalI	TGGCCA	1 cut(s)	602
BanII	GRGCYC	1 cut(s)	1773
BarI	GAAGNNNNNNTAC	4 cut(s)	1179, 1211, 1637, 1669
BauI	CACGAG	1 cut(s)	1323
BbrPI	CACGTG	1 cut(s)	93
BbsI	GAAGAC	4 cut(s)	1440, 1557, 1645, 1671
BbvI	GCAGC	6 cut(s)	11, 120, 367, 845, 1693, 1709
BccI	CCATC	2 cut(s)	1750, 1850
BceAI	ACGGC	2 cut(s)	169, 386
BciT130I	CCWGG	2 cut(s)	380, 846
BciVI	GTATCC	1 cut(s)	733
BclI	TGATCA	1 cut(s)	1807
BcoDI	GTCTC	3 cut(s)	73, 380, 969
BfaI	CTAG	1 cut(s)	1482
BfmI	CTRYAG	4 cut(s)	336, 910, 954, 1440
BfrI	CTTAAG	1 cut(s)	128
BfuAI	ACCTGC	2 cut(s)	349, 1558
BfuI	GTATCC	1 cut(s)	733
BglII	AGATCT	2 cut(s)	914, 1751
BisI	GCNGC	6 cut(s)	25, 109, 356, 834, 1698, 1707
BlsI	GCNGC	6 cut(s)	26, 110, 357, 835, 1699, 1708
BmcAI	AGTACT	1 cut(s)	1646
Bme1390I	CCNGG	2 cut(s)	380, 846
Bme18I	GGWCC	1 cut(s)	1096
BmgT120I	GGNCC	2 cut(s)	860, 1096
BmiI	GGNNCC	2 cut(s)	1338, 1770
BmrFI	CCNGG	2 cut(s)	380, 846
BmsI	GCATC	5 cut(s)	373, 820, 1111, 1227, 1718
BoxI	GACNNNNGTC	1 cut(s)	1356
BpiI	GAAGAC	4 cut(s)	1440, 1557, 1645, 1671
BpmI	CTGGAG	2 cut(s)	362, 1308
Bpu10I	CCTNAGC	1 cut(s)	591
BsaAI	YACGTR	2 cut(s)	93, 1777
BsaBI	GATNNNNATC	2 cut(s)	213, 1505
BsaHI	GRCGYC	1 cut(s)	1149
BsaJI	CCNNGG	3 cut(s)	449, 730, 1018
BsaWI	WCCGGW	1 cut(s)	220
BsaXI	ACNNNNNCTCC	2 cut(s)	219, 249
Bsc4I	CCNNNNNNNGG	7 cut(s)	141, 450, 734, 735, 736, 1019, 1516
Bse118I	RCCGGY	1 cut(s)	1382
Bse1I	ACTGG	4 cut(s)	115, 165, 1098, 1829
Bse8I	GATNNNNATC	2 cut(s)	213, 1505
BseBI	CCWGG	2 cut(s)	380, 846
BseDI	CCNNGG	3 cut(s)	449, 730, 1018
BseGI	GGATG	5 cut(s)	388, 577, 982, 1712, 1742
BseJI	GATNNNNATC	2 cut(s)	213, 1505
BseLI	CCNNNNNNNGG	7 cut(s)	141, 450, 734, 735, 736, 1019, 1516
BseMII	CTCAG	4 cut(s)	560, 909, 918, 1419
BseNI	ACTGG	4 cut(s)	115, 165, 1098, 1829
BsePI	GCGCGC	1 cut(s)	1466
BseRI	GAGGAG	2 cut(s)	935, 1439
BseXI	GCAGC	6 cut(s)	11, 120, 367, 845, 1693, 1709
BseYI	CCCAGC	1 cut(s)	814
BsgI	GTGCAG	1 cut(s)	403
Bsh1236I	CGCG	3 cut(s)	431, 772, 1468
Bsh1285I	CGRYCG	1 cut(s)	15
BshFI	GGCC	4 cut(s)	5, 602, 861, 1616
BsiEI	CGRYCG	1 cut(s)	15
BsiSI	CCGG	3 cut(s)	105, 221, 1383
BslFI	GGGAC	2 cut(s)	1160, 1805
BslI	CCNNNNNNNGG	7 cut(s)	141, 450, 734, 735, 736, 1019, 1516
BsmAI	GTCTC	3 cut(s)	73, 380, 969
BsmBI	CGTCTC	1 cut(s)	73
BsmFI	GGGAC	2 cut(s)	1160, 1805
BsmI	GAATGC	4 cut(s)	819, 866, 1223, 1719
BsnI	GGCC	4 cut(s)	5, 602, 861, 1616
Bsp1286I	GDGCHC	1 cut(s)	1773
Bsp143I	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
BspACI	CCGC	8 cut(s)	11, 58, 204, 252, 325, 429, 1228, 1253
BspANI	GGCC	4 cut(s)	5, 602, 861, 1616
BspCNI	CTCAG	4 cut(s)	559, 910, 919, 1418
BspFNI	CGCG	3 cut(s)	431, 772, 1468
BspLI	GGNNCC	2 cut(s)	1338, 1770
BspMI	ACCTGC	2 cut(s)	349, 1558
BspTI	CTTAAG	1 cut(s)	128
BsrBI	CCGCTC	2 cut(s)	60, 325
BsrFI	RCCGGY	1 cut(s)	1382
BsrI	ACTGG	4 cut(s)	115, 165, 1098, 1829
BssAI	RCCGGY	1 cut(s)	1382
BssECI	CCNNGG	3 cut(s)	449, 730, 1018
BssHII	GCGCGC	1 cut(s)	1466
BssMI	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
BssNI	GRCGYC	1 cut(s)	1149
BssSI	CACGAG	1 cut(s)	1323
BssT1I	CCWWGG	3 cut(s)	449, 730, 1018
Bst2BI	CACGAG	1 cut(s)	1323
Bst2UI	CCWGG	2 cut(s)	380, 846
Bst4CI	ACNGT	7 cut(s)	49, 530, 715, 958, 1195, 1681, 1826
Bst6I	CTCTTC	3 cut(s)	554, 559, 1096
BstACI	GRCGYC	1 cut(s)	1149
BstAFI	CTTAAG	1 cut(s)	128
BstAPI	GCANNNNNTGC	2 cut(s)	24, 1703
BstBAI	YACGTR	2 cut(s)	93, 1777
BstC8I	GCNNGC	5 cut(s)	182, 689, 1063, 1255, 1468
BstDEI	CTNAG	7 cut(s)	546, 591, 756, 918, 927, 1405, 1648
BstENI	CCTNNNNNAGG	1 cut(s)	1514
BstF5I	GGATG	5 cut(s)	388, 577, 982, 1712, 1742
BstFNI	CGCG	3 cut(s)	431, 772, 1468
BstHHI	GCGC	6 cut(s)	37, 313, 433, 1218, 1468, 1470
BstKTI	GATC	5 cut(s)	917, 1208, 1754, 1762, 1810
BstMAI	GTCTC	3 cut(s)	73, 380, 969
BstMBI	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
BstMCI	CGRYCG	1 cut(s)	15
BstMWI	GCNNNNNNNGC	7 cut(s)	24, 117, 190, 258, 1058, 1703, 1706
BstNI	CCWGG	2 cut(s)	380, 846
BstPAI	GACNNNNGTC	1 cut(s)	1356
BstSCI	CCNGG	2 cut(s)	378, 844
BstSFI	CTRYAG	4 cut(s)	336, 910, 954, 1440
BstUI	CGCG	3 cut(s)	431, 772, 1468
BstV1I	GCAGC	6 cut(s)	11, 120, 367, 845, 1693, 1709
BstV2I	GAAGAC	4 cut(s)	1440, 1557, 1645, 1671
BstX2I	RGATCY	2 cut(s)	914, 1751
BstXI	CCANNNNNNTGG	1 cut(s)	586
BstYI	RGATCY	2 cut(s)	914, 1751
BsuI	GTATCC	1 cut(s)	733
BsuRI	GGCC	4 cut(s)	5, 602, 861, 1616
BtgZI	GCGATG	2 cut(s)	1204, 1272
BtsCI	GGATG	5 cut(s)	388, 577, 982, 1712, 1742
BtsI	GCAGTG	1 cut(s)	490
BtsIMutI	CAGTG	5 cut(s)	122, 490, 526, 714, 1412
BveI	ACCTGC	2 cut(s)	349, 1558
Cac8I	GCNNGC	5 cut(s)	182, 689, 1063, 1255, 1468
CaiI	CAGNNNCTG	1 cut(s)	713
CfoI	GCGC	6 cut(s)	37, 313, 433, 1218, 1468, 1470
Cfr10I	RCCGGY	1 cut(s)	1382
Cfr13I	GGNCC	2 cut(s)	860, 1096
Csp6I	GTAC	7 cut(s)	295, 477, 1000, 1091, 1196, 1546, 1645
CspCI	CAANNNNNGTGG	2 cut(s)	660, 695
CviAII	CATG	4 cut(s)	797, 1175, 1555, 1632
CviQI	GTAC	7 cut(s)	295, 477, 1000, 1091, 1196, 1546, 1645
DdeI	CTNAG	7 cut(s)	546, 591, 756, 918, 927, 1405, 1648
DpnI	GATC	5 cut(s)	916, 1207, 1753, 1761, 1809
DpnII	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
EaeI	YGGCCR	2 cut(s)	3, 600
Eam1104I	CTCTTC	3 cut(s)	554, 559, 1096
EarI	CTCTTC	3 cut(s)	554, 559, 1096
Eco130I	CCWWGG	3 cut(s)	449, 730, 1018
Eco24I	GRGCYC	1 cut(s)	1773
Eco32I	GATATC	1 cut(s)	725
Eco47I	GGWCC	1 cut(s)	1096
Eco57I	CTGAAG	1 cut(s)	683
Eco72I	CACGTG	1 cut(s)	93
EcoNI	CCTNNNNNAGG	1 cut(s)	1514
EcoRI	GAATTC	1 cut(s)	650
EcoRII	CCWGG	2 cut(s)	378, 844
EcoRV	GATATC	1 cut(s)	725
EcoT14I	CCWWGG	3 cut(s)	449, 730, 1018
EcoT38I	GRGCYC	1 cut(s)	1773
ErhI	CCWWGG	3 cut(s)	449, 730, 1018
Esp3I	CGTCTC	1 cut(s)	73
FaeI	CATG	4 cut(s)	800, 1178, 1558, 1635
FalI	AAGNNNNNCTT	2 cut(s)	1686, 1718
FaqI	GGGAC	2 cut(s)	1160, 1805
FatI	CATG	4 cut(s)	796, 1174, 1554, 1631
FauI	CCCGC	1 cut(s)	1246
FauNDI	CATATG	1 cut(s)	790
FbaI	TGATCA	1 cut(s)	1807
FblI	GTMKAC	1 cut(s)	909
Fnu4HI	GCNGC	6 cut(s)	25, 109, 356, 834, 1698, 1707
FokI	GGATG	5 cut(s)	395, 584, 989, 1699, 1729
FriOI	GRGCYC	1 cut(s)	1773
Fsp4HI	GCNGC	6 cut(s)	25, 109, 356, 834, 1698, 1707
FspBI	CTAG	1 cut(s)	1482
GlaI	GCGC	6 cut(s)	36, 312, 432, 1217, 1467, 1469
GluI	GCNGC	6 cut(s)	25, 109, 356, 834, 1698, 1707
GsaI	CCCAGC	1 cut(s)	818
GsuI	CTGGAG	2 cut(s)	362, 1308
HaeIII	GGCC	4 cut(s)	5, 602, 861, 1616
HapII	CCGG	3 cut(s)	105, 221, 1383
HhaI	GCGC	6 cut(s)	37, 313, 433, 1218, 1468, 1470
Hin1I	GRCGYC	1 cut(s)	1149
Hin1II	CATG	4 cut(s)	800, 1178, 1558, 1635
Hin6I	GCGC	6 cut(s)	35, 311, 431, 1216, 1466, 1468
HinP1I	GCGC	6 cut(s)	35, 311, 431, 1216, 1466, 1468
HindIII	AAGCTT	2 cut(s)	685, 1159
HinfI	GANTC	9 cut(s)	332, 704, 1045, 1076, 1436, 1485, 1628, 1738, 1781
HpaII	CCGG	3 cut(s)	105, 221, 1383
HphI	GGTGA	2 cut(s)	31, 512
Hpy166II	GTNNAC	6 cut(s)	415, 910, 1002, 1091, 1395, 1537
Hpy188III	TCNNGA	5 cut(s)	171, 514, 655, 701, 1811
Hpy8I	GTNNAC	6 cut(s)	415, 910, 1002, 1091, 1395, 1537
Hpy99I	CGWCG	4 cut(s)	190, 193, 202, 1355
HpyAV	CCTTC	8 cut(s)	73, 91, 224, 265, 322, 455, 1181, 1374
HpyCH4III	ACNGT	7 cut(s)	49, 530, 715, 958, 1195, 1681, 1826
HpyCH4IV	ACGT	3 cut(s)	92, 1149, 1776
HpyF10VI	GCNNNNNNNGC	7 cut(s)	24, 117, 190, 258, 1058, 1703, 1706
HpyF3I	CTNAG	7 cut(s)	546, 591, 756, 918, 927, 1405, 1648
HpySE526I	ACGT	3 cut(s)	92, 1149, 1776
Hsp92I	GRCGYC	1 cut(s)	1149
Hsp92II	CATG	4 cut(s)	800, 1178, 1558, 1635
HspAI	GCGC	6 cut(s)	35, 311, 431, 1216, 1466, 1468
Ksp22I	TGATCA	1 cut(s)	1807
Kzo9I	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
LmnI	GCTCC	5 cut(s)	65, 144, 308, 662, 1768
Lsp1109I	GCAGC	6 cut(s)	11, 120, 367, 845, 1693, 1709
LweI	GCATC	5 cut(s)	373, 820, 1111, 1227, 1718
MaeI	CTAG	1 cut(s)	1482
MaeII	ACGT	3 cut(s)	92, 1149, 1776
MaeIII	GTNAC	4 cut(s)	500, 709, 751, 781
MalI	GATC	5 cut(s)	916, 1207, 1753, 1761, 1809
MbiI	CCGCTC	2 cut(s)	60, 325
MboI	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
MfeI	CAATTG	1 cut(s)	677
MflI	RGATCY	2 cut(s)	914, 1751
MhlI	GDGCHC	1 cut(s)	1773
MlsI	TGGCCA	1 cut(s)	602
MluCI	AATT	6 cut(s)	650, 677, 1247, 1377, 1837, 1860
MluNI	TGGCCA	1 cut(s)	602
MlyI	GAGTC	3 cut(s)	341, 1085, 1430
MmeI	TCCRAC	4 cut(s)	100, 122, 1020, 1746
Mox20I	TGGCCA	1 cut(s)	602
MroXI	GAANNNNTTC	1 cut(s)	234
MscI	TGGCCA	1 cut(s)	602
MslI	CAYNNNNRTG	1 cut(s)	893
Msp20I	TGGCCA	1 cut(s)	602
MspA1I	CMGCKG	1 cut(s)	206
MspCI	CTTAAG	1 cut(s)	128
MspI	CCGG	3 cut(s)	105, 221, 1383
MspR9I	CCNGG	2 cut(s)	380, 846
MunI	CAATTG	1 cut(s)	677
Mva1269I	GAATGC	4 cut(s)	819, 866, 1223, 1719
MvaI	CCWGG	2 cut(s)	380, 846
MvnI	CGCG	3 cut(s)	431, 772, 1468
MwoI	GCNNNNNNNGC	7 cut(s)	24, 117, 190, 258, 1058, 1703, 1706
NdeI	CATATG	1 cut(s)	790
NdeII	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
NlaIII	CATG	4 cut(s)	800, 1178, 1558, 1635
NlaIV	GGNNCC	2 cut(s)	1338, 1770
NmuCI	GTSAC	2 cut(s)	500, 709
PauI	GCGCGC	1 cut(s)	1466
PcsI	WCGNNNNNNNCGW	2 cut(s)	194, 197
PctI	GAATGC	4 cut(s)	819, 866, 1223, 1719
PdmI	GAANNNNTTC	1 cut(s)	234
PfeI	GAWTC	6 cut(s)	704, 1045, 1485, 1628, 1738, 1781
PfoI	TCCNGGA	1 cut(s)	378
PkrI	GCNGC	6 cut(s)	26, 110, 357, 835, 1699, 1708
PleI	GAGTC	3 cut(s)	340, 1084, 1430
PmaCI	CACGTG	1 cut(s)	93
PmlI	CACGTG	1 cut(s)	93
PpsI	GAGTC	3 cut(s)	340, 1084, 1430
Ppu21I	YACGTR	2 cut(s)	93, 1777
PshAI	GACNNNNGTC	1 cut(s)	1356
Psp6I	CCWGG	2 cut(s)	378, 844
PspCI	CACGTG	1 cut(s)	93
PspFI	CCCAGC	1 cut(s)	814
PspGI	CCWGG	2 cut(s)	378, 844
PspN4I	GGNNCC	2 cut(s)	1338, 1770
PspPI	GGNCC	2 cut(s)	860, 1096
PsrI	GAACNNNNNNTAC	4 cut(s)	287, 319, 983, 1015
PstNI	CAGNNNCTG	1 cut(s)	713
PsuI	RGATCY	2 cut(s)	914, 1751
PteI	GCGCGC	1 cut(s)	1466
RsaI	GTAC	7 cut(s)	296, 478, 1001, 1092, 1197, 1547, 1646
RsaNI	GTAC	7 cut(s)	295, 477, 1000, 1091, 1196, 1546, 1645
RseI	CAYNNNNRTG	1 cut(s)	893
SatI	GCNGC	6 cut(s)	25, 109, 356, 834, 1698, 1707
Sau3AI	GATC	5 cut(s)	914, 1205, 1751, 1759, 1807
Sau96I	GGNCC	2 cut(s)	860, 1096
ScaI	AGTACT	1 cut(s)	1646
SchI	GAGTC	3 cut(s)	341, 1085, 1430
ScrFI	CCNGG	2 cut(s)	380, 846
SduI	GDGCHC	1 cut(s)	1773
SfaNI	GCATC	5 cut(s)	373, 820, 1111, 1227, 1718
SfcI	CTRYAG	4 cut(s)	336, 910, 954, 1440
SinI	GGWCC	1 cut(s)	1096
SmiMI	CAYNNNNRTG	1 cut(s)	893
SmlI	CTYRAG	1 cut(s)	128
SmoI	CTYRAG	1 cut(s)	128
Sse9I	AATT	6 cut(s)	650, 677, 1247, 1377, 1837, 1860
SsiI	CCGC	8 cut(s)	11, 58, 204, 252, 325, 429, 1228, 1253
SspI	AATATT	1 cut(s)	934
SspMI	CTAG	1 cut(s)	1482
StyD4I	CCNGG	2 cut(s)	378, 844
StyI	CCWWGG	3 cut(s)	449, 730, 1018
TaaI	ACNGT	7 cut(s)	49, 530, 715, 958, 1195, 1681, 1826
TaiI	ACGT	3 cut(s)	95, 1152, 1779
TaqI	TCGA	5 cut(s)	170, 1204, 1350, 1656, 1854
TasI	AATT	6 cut(s)	650, 677, 1247, 1377, 1837, 1860
TatI	WGTACW	4 cut(s)	476, 999, 1090, 1644
TfiI	GAWTC	6 cut(s)	704, 1045, 1485, 1628, 1738, 1781
TscAI	CASTG	5 cut(s)	122, 490, 533, 714, 1419
TseFI	GTSAC	2 cut(s)	500, 709
TseI	GCWGC	6 cut(s)	24, 108, 355, 833, 1697, 1706
Tsp45I	GTSAC	2 cut(s)	500, 709
TspDTI	ATGAA	6 cut(s)	1163, 1313, 1334, 1545, 1595, 1620
TspGWI	ACGGA	4 cut(s)	61, 186, 1368, 1599
TspRI	CASTG	5 cut(s)	122, 490, 533, 714, 1419
Vha464I	CTTAAG	1 cut(s)	128
VpaK11BI	GGWCC	1 cut(s)	1096
XagI	CCTNNNNNAGG	1 cut(s)	1514
XapI	RAATTY	3 cut(s)	650, 1247, 1837
XmiI	GTMKAC	1 cut(s)	909
XmnI	GAANNNNTTC	1 cut(s)	234
XspI	CTAG	1 cut(s)	1482
ZraI	GACGTC	1 cut(s)	1150
ZrmI	AGTACT	1 cut(s)	1646

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.