Rorug06G0228000

Protease Do-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0007826
Physical Location & Seq
Reverse (-)
36616992 .. 36618306
1315 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0228000.1

Sequence Viewer

Length: 375 bp
ATGTTTTGGAATCAAGAGCAAGTCAATGGAATTATAATCCCTAGATTCAGAATGTTGTGTGCCATGAAGAGAAACATCAGGTCTCAATCGCAATTTAGGTACCGCGCCAATTCTATCATGGTGGACCACGAGGGACTAGCTGAATATAAGAAGAAGAAGAAGGAGAATGATATACCACGCATTGCCAAGGCTAAAGCTAGAGATATATATAATGGAATAAAGCTTATTGCAGAACACAGAGCATTGGAGGACCCATCAGAGCATTCAGAAAAGTGGAAGCGTATATCAAAGAATTTGTCAGATGGTGTGCTTACAAAAAAAAGTCTCAGGGCTGCACTTATAAAAGAATATCAAGCTCAGGTGAGATGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PDZ_2 Trypsin_2

Protein Analysis

124

Amino Acids

14.59

Weight (kDa)

10.05

Isoelectric Point (pI)

26.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 35, 341
Acc65I GGTACC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 99
AccII CGCG 1 cut(s) 105
AciI CCGC 1 cut(s) 103
AcsI RAATTY 1 cut(s) 292
AfaI GTAC 1 cut(s) 101
AluBI AGCT 4 cut(s) 140, 197, 223, 356
AluI AGCT 4 cut(s) 140, 197, 223, 356
Alw26I GTCTC 2 cut(s) 87, 329
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 292
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp718I GGTACC 1 cut(s) 99
AspLEI GCGC 1 cut(s) 107
AspS9I GGNCC 2 cut(s) 124, 250
AsuHPI GGTGA 1 cut(s) 373
AvaII GGWCC 2 cut(s) 124, 250
BanI GGYRCC 1 cut(s) 99
BauI CACGAG 1 cut(s) 128
BbvI GCAGC 1 cut(s) 319
BccI CCATC 2 cut(s) 262, 296
BcoDI GTCTC 2 cut(s) 87, 329
BfaI CTAG 3 cut(s) 42, 137, 198
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bme18I GGWCC 2 cut(s) 124, 250
BmgT120I GGNCC 2 cut(s) 124, 250
BmiI GGNNCC 2 cut(s) 101, 252
BmsI GCATC 1 cut(s) 356
Bpu10I CCTNAGC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 87
BsaJI CCNNGG 1 cut(s) 186
Bse3DI GCAATG 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 180
BseMII CTCAG 2 cut(s) 340, 371
BseXI GCAGC 1 cut(s) 319
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 105
BshNI GGYRCC 1 cut(s) 99
BslFI GGGAC 1 cut(s) 147
BsmAI GTCTC 2 cut(s) 87, 329
BsmFI GGGAC 1 cut(s) 147
BsmI GAATGC 1 cut(s) 262
Bso31I GGTCTC 1 cut(s) 87
BspACI CCGC 1 cut(s) 103
BspCNI CTCAG 2 cut(s) 339, 370
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 2 cut(s) 101, 252
BspT107I GGYRCC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 87
BsrDI GCAATG 1 cut(s) 180
BssECI CCNNGG 1 cut(s) 186
BssSI CACGAG 1 cut(s) 128
BssT1I CCWWGG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 128
Bst6I CTCTTC 1 cut(s) 62
BstDEI CTNAG 2 cut(s) 326, 357
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 107
BstMAI GTCTC 2 cut(s) 87, 329
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 1 cut(s) 319
CfoI GCGC 1 cut(s) 107
Cfr13I GGNCC 2 cut(s) 124, 250
Csp6I GTAC 1 cut(s) 100
CviAII CATG 2 cut(s) 64, 118
CviJI RGCY 6 cut(s) 140, 191, 197, 223, 332, 356
CviKI_1 RGCY 6 cut(s) 140, 191, 197, 223, 332, 356
CviQI GTAC 1 cut(s) 100
DdeI CTNAG 2 cut(s) 326, 357
Eam1104I CTCTTC 1 cut(s) 62
EarI CTCTTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 186
Eco31I GGTCTC 1 cut(s) 87
Eco47I GGWCC 2 cut(s) 124, 250
EcoO109I RGGNCCY 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 2 cut(s) 67, 121
FaqI GGGAC 1 cut(s) 147
FatI CATG 2 cut(s) 63, 117
Fnu4HI GCNGC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 333
FspBI CTAG 3 cut(s) 42, 137, 198
GlaI GCGC 1 cut(s) 106
GluI GCNGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 107
Hin1II CATG 2 cut(s) 67, 121
Hin6I GCGC 1 cut(s) 105
HinP1I GCGC 1 cut(s) 105
HindIII AAGCTT 1 cut(s) 221
HinfI GANTC 2 cut(s) 10, 45
HphI GGTGA 1 cut(s) 373
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 50, 259, 268, 301
Hpy188III TCNNGA 1 cut(s) 14
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 1 cut(s) 154
HpyCH4V TGCA 2 cut(s) 230, 335
HpyF3I CTNAG 2 cut(s) 326, 357
Hsp92II CATG 2 cut(s) 67, 121
HspAI GCGC 1 cut(s) 105
KpnI GGTACC 1 cut(s) 103
LpnPI CCDG 3 cut(s) 64, 313, 344
Lsp1109I GCAGC 1 cut(s) 319
LweI GCATC 1 cut(s) 356
MaeI CTAG 3 cut(s) 42, 137, 198
MboII GAAGA 4 cut(s) 79, 163, 166, 169
MluCI AATT 4 cut(s) 30, 92, 109, 292
MnlI CCTC 2 cut(s) 124, 241
Mva1269I GAATGC 1 cut(s) 262
MvnI CGCG 1 cut(s) 105
NlaIII CATG 2 cut(s) 67, 121
NlaIV GGNNCC 2 cut(s) 101, 252
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 2 cut(s) 10, 45
PkrI GCNGC 1 cut(s) 334
PpuMI RGGWCCY 1 cut(s) 250
PsiI TTATAA 2 cut(s) 35, 341
Psp5II RGGWCCY 1 cut(s) 250
PspN4I GGNNCC 2 cut(s) 101, 252
PspPI GGNCC 2 cut(s) 124, 250
PspPPI RGGWCCY 1 cut(s) 250
RsaI GTAC 1 cut(s) 101
RsaNI GTAC 1 cut(s) 100
SatI GCNGC 1 cut(s) 333
Sau96I GGNCC 2 cut(s) 124, 250
SetI ASST 7 cut(s) 83, 101, 142, 199, 225, 358, 363
SfaNI GCATC 1 cut(s) 356
SinI GGWCC 2 cut(s) 124, 250
Sse9I AATT 4 cut(s) 30, 92, 109, 292
SsiI CCGC 1 cut(s) 103
SspMI CTAG 3 cut(s) 42, 137, 198
StyI CCWWGG 1 cut(s) 186
TasI AATT 4 cut(s) 30, 92, 109, 292
TfiI GAWTC 2 cut(s) 10, 45
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 124, 250
XapI RAATTY 1 cut(s) 292
XcmI CCANNNNNNNNNTGG 1 cut(s) 115
XspI CTAG 3 cut(s) 42, 137, 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.