Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0014681

Physical Location & Seq

Genomic Coordinates Reverse (-)

10582886 .. 10585532

Gene Structure 2647 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1057000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGGCTAGTGATTTTAGCGATGATGTTAAACAAACCTTACTTTCTGAAAAGAAAGATAATTCTCAACACAATAATGAAACGAATGCCGTCCAGAGAAGAAGAAATCGCCCATACAGCATTAGCACTAGTCCATGTCCATCATTCAAGAACCGCCTACAACAAAACGAAGTGGTATCTCAATATCCTGCAGATTGTGATCCAGTCTATGTAAAACCACAATTCAGTCTAAGGCAAGTAGTCTTGTTGTTGATTGCATACATTGGTGGAGGCACATTTTGCTTTTTCCTGATGAGGCATCAGATAAAGGGTAAGAAAACGAATGCAATTCTGGACTCCATGTACTTGTGTGTAGTCACAATGAGCACTGTGGGCTACGGGGACCTTGTGCCAAATAGCATGTTGGCAAAGCTAGTTGCTTGCGTTTATGTGTTCACCGGCATGGCCCTTGTTGGCATAATTCTTGGCAAGGCAGCAGATTACCTTGTAGAGAAACAGGAAATCCTTTTGGTTAGAGCCATACATTTTCGAGAAAAAGTTGGGCCACCGGAGCTTCTTAAGGAGGTTGAGACCGAAAAGGTTAAATTCAAATGTATTTCTGTCGGAATCCTTCTTTTGATTCTTATTATAGTAGGAACTGTCTTCTTATGTCTAGTTGAGAATCTGGAAGTTATGGATGCTTTCTATTGCGTTTGTTCCACTATTACTACACTAGGCTATGGGGACGAGAGCTTCTCAACGGGAGCTGGTCGTATTTTCGCTGTTTTTTGGATACTGAGCAGCACCATTTGTTTAGCACAGTTCTTTCTCTACCTTGCTGAACTATGCACTGAAAGAAGGCAAAGATCACTTGTGAAGTGGGCTCTTACGCGGAGATTAACACCCTCGGATCTTGAGGAAGCAGATCTTGATCATGATAAAGTCGTTAGTGCGGCAGAGTTTATCGTATATAAGCTAAAGGAGATGGGGAAGATCAGCCAGGAAGATATTGAACTGCTGATGGAAACGTTCAAGAAACTTGACATTGATCATTCAGGAACTTTGACGACATCTGATTTAATATCAATAATTCCAGGAGGGAGCTCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008324 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ion_trans_2

Protein Analysis

362

Amino Acids

40.4

Weight (kDa)

6.03

Isoelectric Point (pI)

37.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans_2	PF07885	82 - 162	2.6e-16	Ion channel
Ion_trans	PF00520	103 - 157	2.2e-06	Ion transport protein
Ion_trans_2	PF07885	202 - 276	5.1e-14	Ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014681)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g00500 FvH4_3g00500
malus_domestica	MD00G1057000.v1.1 MD10G1337300.v1.1
prunus_persica	Prupe.4G005900_v2.0.a1 Prupe.4G005900_v2.0.a1 Prupe.4G005900_v2.0.a1
pyrus_communis	pycom10g28670
rosa_chinensis	RchiOBHm_Chr5g0000671
rosa_laevigata	RLG00000030886
rosa_multiflora	Rmu_sc0011992.1_g000010
rosa_rugosa	Rorug04G0385500
rosa_samantha	Rh5AG005600 Rh5BG007200 Rh5CG006000 Rh5DG006000
rosa_wichuraiana	Rw5G000570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	868
AciI	CCGC	3 cut(s)	151, 868, 929
AclI	AACGTT	1 cut(s)	1004
AclWI	GGATC	2 cut(s)	191, 894
AcsI	RAATTY	1 cut(s)	581
AfaI	GTAC	1 cut(s)	341
AflII	CTTAAG	1 cut(s)	554
AgsI	TTSAA	4 cut(s)	145, 586, 989, 1009
AhlI	ACTAGT	1 cut(s)	125
AjnI	CCWGG	2 cut(s)	975, 1069
AluBI	AGCT	6 cut(s)	409, 550, 729, 743, 952, 1080
AluI	AGCT	6 cut(s)	409, 550, 729, 743, 952, 1080
Alw21I	GWGCWC	2 cut(s)	365, 1082
Alw26I	GTCTC	1 cut(s)	560
AlwI	GGATC	2 cut(s)	191, 894
AoxI	GGCC	2 cut(s)	441, 539
ApeKI	GCWGC	2 cut(s)	470, 777
ApoI	RAATTY	1 cut(s)	581
AspS9I	GGNCC	3 cut(s)	379, 442, 539
AsuHPI	GGTGA	1 cut(s)	424
AvaII	GGWCC	1 cut(s)	379
BanII	GRGCYC	2 cut(s)	862, 1082
BbsI	GAAGAC	1 cut(s)	631
Bbv12I	GWGCWC	2 cut(s)	365, 1082
BbvI	GCAGC	2 cut(s)	482, 789
BccI	CCATC	3 cut(s)	145, 955, 991
BceAI	ACGGC	1 cut(s)	71
BciT130I	CCWGG	2 cut(s)	977, 1071
BciVI	GTATCC	1 cut(s)	762
BclI	TGATCA	2 cut(s)	907, 1024
BcoDI	GTCTC	1 cut(s)	560
BcuI	ACTAGT	1 cut(s)	125
BfaI	CTAG	5 cut(s)	6, 126, 410, 650, 710
BfmI	CTRYAG	1 cut(s)	186
BfrI	CTTAAG	1 cut(s)	554
BfuI	GTATCC	1 cut(s)	762
BglII	AGATCT	1 cut(s)	901
BisI	GCNGC	3 cut(s)	471, 778, 930
BlsI	GCNGC	3 cut(s)	472, 779, 931
Bme1390I	CCNGG	2 cut(s)	977, 1071
Bme18I	GGWCC	1 cut(s)	379
BmgT120I	GGNCC	3 cut(s)	379, 442, 539
BmiI	GGNNCC	1 cut(s)	380
BmrFI	CCNGG	2 cut(s)	977, 1071
BmsI	GCATC	2 cut(s)	304, 664
BpiI	GAAGAC	1 cut(s)	631
BplI	GAGNNNNNCTC	2 cut(s)	716, 748
BpuEI	CTTGAG	1 cut(s)	911
BsaBI	GATNNNNATC	2 cut(s)	195, 906
BsaI	GGTCTC	1 cut(s)	560
BsaJI	CCNNGG	1 cut(s)	882
BsaWI	WCCGGW	1 cut(s)	544
Bse118I	RCCGGY	1 cut(s)	434
Bse1I	ACTGG	1 cut(s)	200
Bse8I	GATNNNNATC	2 cut(s)	195, 906
BseBI	CCWGG	2 cut(s)	977, 1071
BseDI	CCNNGG	1 cut(s)	882
BseGI	GGATG	1 cut(s)	679
BseJI	GATNNNNATC	2 cut(s)	195, 906
BseMII	CTCAG	1 cut(s)	764
BseNI	ACTGG	1 cut(s)	200
BseXI	GCAGC	2 cut(s)	482, 789
Bsh1236I	CGCG	1 cut(s)	868
BshFI	GGCC	2 cut(s)	443, 541
BsiHKAI	GWGCWC	2 cut(s)	365, 1082
BsiSI	CCGG	2 cut(s)	435, 545
BslFI	GGGAC	2 cut(s)	392, 734
BsmAI	GTCTC	1 cut(s)	560
BsmFI	GGGAC	2 cut(s)	392, 734
BsmI	GAATGC	2 cut(s)	88, 325
BsnI	GGCC	2 cut(s)	443, 541
Bso31I	GGTCTC	1 cut(s)	560
Bsp1286I	GDGCHC	3 cut(s)	365, 862, 1082
Bsp143I	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
BspACI	CCGC	3 cut(s)	151, 868, 929
BspANI	GGCC	2 cut(s)	443, 541
BspCNI	CTCAG	1 cut(s)	765
BspFNI	CGCG	1 cut(s)	868
BspHI	TCATGA	1 cut(s)	910
BspLI	GGNNCC	1 cut(s)	380
BspMAI	CTGCAG	1 cut(s)	190
BspPI	GGATC	2 cut(s)	191, 894
BspTI	CTTAAG	1 cut(s)	554
BspTNI	GGTCTC	1 cut(s)	560
BsrFI	RCCGGY	1 cut(s)	434
BsrI	ACTGG	1 cut(s)	200
BssAI	RCCGGY	1 cut(s)	434
BssECI	CCNNGG	1 cut(s)	882
BssMI	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
Bst2UI	CCWGG	2 cut(s)	977, 1071
Bst4CI	ACNGT	3 cut(s)	367, 637, 798
BstAFI	CTTAAG	1 cut(s)	554
BstAPI	GCANNNNNTGC	1 cut(s)	276
BstC8I	GCNNGC	1 cut(s)	418
BstDEI	CTNAG	2 cut(s)	227, 773
BstF5I	GGATG	1 cut(s)	679
BstFNI	CGCG	1 cut(s)	868
BstKTI	GATC	7 cut(s)	199, 845, 889, 904, 910, 972, 1027
BstMAI	GTCTC	1 cut(s)	560
BstMBI	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
BstMWI	GCNNNNNNNGC	4 cut(s)	114, 276, 369, 547
BstNI	CCWGG	2 cut(s)	977, 1071
BstNSI	RCATGY	1 cut(s)	400
BstSCI	CCNGG	2 cut(s)	975, 1069
BstSFI	CTRYAG	1 cut(s)	186
BstUI	CGCG	1 cut(s)	868
BstV1I	GCAGC	2 cut(s)	482, 789
BstV2I	GAAGAC	1 cut(s)	631
BstX2I	RGATCY	2 cut(s)	886, 901
BstYI	RGATCY	2 cut(s)	886, 901
BsuI	GTATCC	1 cut(s)	762
BsuRI	GGCC	2 cut(s)	443, 541
BtgZI	GCGATG	1 cut(s)	33
BtsCI	GGATG	1 cut(s)	679
BtsIMutI	CAGTG	2 cut(s)	363, 825
Cac8I	GCNNGC	1 cut(s)	418
CciI	TCATGA	1 cut(s)	910
Cfr10I	RCCGGY	1 cut(s)	434
Cfr13I	GGNCC	3 cut(s)	379, 442, 539
Csp6I	GTAC	1 cut(s)	340
CviAII	CATG	5 cut(s)	132, 337, 397, 439, 911
CviQI	GTAC	1 cut(s)	340
DdeI	CTNAG	2 cut(s)	227, 773
DpnI	GATC	7 cut(s)	198, 844, 888, 903, 909, 971, 1026
DpnII	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
Ecl136II	GAGCTC	1 cut(s)	1080
Eco24I	GRGCYC	2 cut(s)	862, 1082
Eco31I	GGTCTC	1 cut(s)	560
Eco47I	GGWCC	1 cut(s)	379
Eco53kI	GAGCTC	1 cut(s)	1080
EcoICRI	GAGCTC	1 cut(s)	1080
EcoO109I	RGGNCCY	1 cut(s)	379
EcoRII	CCWGG	2 cut(s)	975, 1069
EcoT38I	GRGCYC	2 cut(s)	862, 1082
FaeI	CATG	5 cut(s)	135, 340, 400, 442, 914
FalI	AAGNNNNNCTT	2 cut(s)	888, 920
FaqI	GGGAC	2 cut(s)	392, 734
FatI	CATG	5 cut(s)	131, 336, 396, 438, 910
FbaI	TGATCA	2 cut(s)	907, 1024
Fnu4HI	GCNGC	3 cut(s)	471, 778, 930
FokI	GGATG	1 cut(s)	686
FriOI	GRGCYC	2 cut(s)	862, 1082
Fsp4HI	GCNGC	3 cut(s)	471, 778, 930
FspBI	CTAG	5 cut(s)	6, 126, 410, 650, 710
GluI	GCNGC	3 cut(s)	471, 778, 930
HaeIII	GGCC	2 cut(s)	443, 541
HapII	CCGG	2 cut(s)	435, 545
Hin1II	CATG	5 cut(s)	135, 340, 400, 442, 914
HinfI	GANTC	4 cut(s)	332, 603, 616, 658
HpaII	CCGG	2 cut(s)	435, 545
HphI	GGTGA	1 cut(s)	424
Hpy166II	GTNNAC	1 cut(s)	432
Hpy188I	TCNGA	5 cut(s)	46, 300, 602, 886, 1051
Hpy8I	GTNNAC	1 cut(s)	432
HpyAV	CCTTC	2 cut(s)	617, 828
HpyCH4III	ACNGT	3 cut(s)	367, 637, 798
HpyCH4IV	ACGT	1 cut(s)	1004
HpyCH4V	TGCA	4 cut(s)	188, 254, 323, 825
HpyF10VI	GCNNNNNNNGC	4 cut(s)	114, 276, 369, 547
HpyF3I	CTNAG	2 cut(s)	227, 773
HpySE526I	ACGT	1 cut(s)	1004
Hsp92II	CATG	5 cut(s)	135, 340, 400, 442, 914
Ksp22I	TGATCA	2 cut(s)	907, 1024
Kzo9I	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
LmnI	GCTCC	3 cut(s)	547, 740, 1077
Lsp1109I	GCAGC	2 cut(s)	482, 789
LweI	GCATC	2 cut(s)	304, 664
MaeI	CTAG	5 cut(s)	6, 126, 410, 650, 710
MaeII	ACGT	1 cut(s)	1004
MaeIII	GTNAC	1 cut(s)	352
MalI	GATC	7 cut(s)	198, 844, 888, 903, 909, 971, 1026
MboI	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
MboII	GAAGA	5 cut(s)	108, 111, 631, 979, 992
MflI	RGATCY	2 cut(s)	886, 901
MhlI	GDGCHC	3 cut(s)	365, 862, 1082
MluCI	AATT	6 cut(s)	58, 218, 324, 456, 581, 1065
MlyI	GAGTC	1 cut(s)	326
MmeI	TCCRAC	1 cut(s)	580
MnlI	CCTC	6 cut(s)	260, 285, 553, 886, 892, 1067
MseI	TTAA	5 cut(s)	27, 555, 579, 875, 1055
MslI	CAYNNNNRTG	2 cut(s)	72, 437
MspCI	CTTAAG	1 cut(s)	554
MspI	CCGG	2 cut(s)	435, 545
MspR9I	CCNGG	2 cut(s)	977, 1071
Mva1269I	GAATGC	2 cut(s)	88, 325
MvaI	CCWGG	2 cut(s)	977, 1071
MvnI	CGCG	1 cut(s)	868
MwoI	GCNNNNNNNGC	4 cut(s)	114, 276, 369, 547
NdeII	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
NlaIII	CATG	5 cut(s)	135, 340, 400, 442, 914
NlaIV	GGNNCC	1 cut(s)	380
NmuCI	GTSAC	1 cut(s)	352
NspI	RCATGY	1 cut(s)	400
PagI	TCATGA	1 cut(s)	910
PctI	GAATGC	2 cut(s)	88, 325
PfeI	GAWTC	3 cut(s)	603, 616, 658
PfoI	TCCNGGA	1 cut(s)	1069
PkrI	GCNGC	3 cut(s)	472, 779, 931
PleI	GAGTC	1 cut(s)	326
PpsI	GAGTC	1 cut(s)	326
PpuMI	RGGWCCY	1 cut(s)	379
Psp124BI	GAGCTC	1 cut(s)	1082
Psp1406I	AACGTT	1 cut(s)	1004
Psp5II	RGGWCCY	1 cut(s)	379
Psp6I	CCWGG	2 cut(s)	975, 1069
PspGI	CCWGG	2 cut(s)	975, 1069
PspN4I	GGNNCC	1 cut(s)	380
PspPI	GGNCC	3 cut(s)	379, 442, 539
PspPPI	RGGWCCY	1 cut(s)	379
PstI	CTGCAG	1 cut(s)	190
PsuI	RGATCY	2 cut(s)	886, 901
RsaI	GTAC	1 cut(s)	341
RsaNI	GTAC	1 cut(s)	340
RseI	CAYNNNNRTG	2 cut(s)	72, 437
SacI	GAGCTC	1 cut(s)	1082
SaqAI	TTAA	5 cut(s)	27, 555, 579, 875, 1055
SatI	GCNGC	3 cut(s)	471, 778, 930
Sau3AI	GATC	7 cut(s)	196, 842, 886, 901, 907, 969, 1024
Sau96I	GGNCC	3 cut(s)	379, 442, 539
SchI	GAGTC	1 cut(s)	326
ScrFI	CCNGG	2 cut(s)	977, 1071
SduI	GDGCHC	3 cut(s)	365, 862, 1082
SfaNI	GCATC	2 cut(s)	304, 664
SfcI	CTRYAG	1 cut(s)	186
SinI	GGWCC	1 cut(s)	379
SmiMI	CAYNNNNRTG	2 cut(s)	72, 437
SmlI	CTYRAG	2 cut(s)	554, 890
SmoI	CTYRAG	2 cut(s)	554, 890
SpeI	ACTAGT	1 cut(s)	125
Sse9I	AATT	6 cut(s)	58, 218, 324, 456, 581, 1065
SsiI	CCGC	3 cut(s)	151, 868, 929
SspMI	CTAG	5 cut(s)	6, 126, 410, 650, 710
SstI	GAGCTC	1 cut(s)	1082
StyD4I	CCNGG	2 cut(s)	975, 1069
TaaI	ACNGT	3 cut(s)	367, 637, 798
TaiI	ACGT	1 cut(s)	1007
TaqI	TCGA	1 cut(s)	526
TaqII	GACCGA	1 cut(s)	584
TasI	AATT	6 cut(s)	58, 218, 324, 456, 581, 1065
TatI	WGTACW	1 cut(s)	339
TauI	GCSGC	1 cut(s)	932
TfiI	GAWTC	3 cut(s)	603, 616, 658
Tru1I	TTAA	5 cut(s)	27, 555, 579, 875, 1055
Tru9I	TTAA	5 cut(s)	27, 555, 579, 875, 1055
TscAI	CASTG	2 cut(s)	370, 832
TseFI	GTSAC	1 cut(s)	352
TseI	GCWGC	2 cut(s)	470, 777
Tsp45I	GTSAC	1 cut(s)	352
TspDTI	ATGAA	1 cut(s)	90
TspRI	CASTG	2 cut(s)	370, 832
Vha464I	CTTAAG	1 cut(s)	554
VpaK11BI	GGWCC	1 cut(s)	379
XapI	RAATTY	1 cut(s)	581
XceI	RCATGY	1 cut(s)	400
XspI	CTAG	5 cut(s)	6, 126, 410, 650, 710

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.