MD10G1337300.v1.1

Potassium channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0014681
Physical Location & Seq
Forward (+)
41337250 .. 41338786
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1337300.v1.1.491

Sequence Viewer

Length: 1086 bp
ATGGCTACTGATTTTAGCGACGATGTTAAAGAAGCCTTACTTTCTGAAAAGAAAGATCATTCTCAACTCAATACTGAAACAAATGTCGTCCATAGAAGAAGAAATCGCCTTTATAGCATTAGCACTACCCCATGTCCATCATTCAAGAACCGCGTACAACAAAATGAAGCGGAATCTCAATGTCCTGTAGATTCTGGTCCGCTCTATGTAAAACCACAATTCAGTCTAACGCAAGTACTTTTGTTGTTGATTGCATACATTGGTGGAGGCACATTTTGCTTTTTCCTGATGAGGCATCAGATAAAGGGTAAGAAAACGAATGCCATTCTGGACTCCATGTACTTGTGTATAGTCACAATGAGCACTGTTGGTTACGGGGACCTTGTGCCAGATAGCATGTTGGCAAAGCTAGTTGCATGCGTTTATGTGTTCATCGGCATGGCCCTTGTTGGCATAATTCTTGGCAAGGCAGCAGATTACCTTGTAGAGAAGCAGGAAATCCTTTTGGTTAGAGCTATACATTTTCGCGAAAAATTTGGGCCACCGGAGCTTCTTAAGGAGGTTGAGACCGAAAAAGTCAAGTTCAAATGTATTACTGTCGGAATCCTTCTTTTGATTCTTATTATAGTAGGTACTGTCTTCTTATGTCTAGTTGAGAATCTGGAAGTTACGGATGCTCTCTATTGCGTTTGTTCCACTATAACTACACTAGGCTATGGGGACGAGAGTTTCTCAACGGGAGCTGGTCGTATTTTCGCTGTTTTTTGGATACTGAGCAGCACCATTTGTTTAGCACAGTTCTTTCTCTACCTTGCTGAACTATACACTGAAAGAAGGCAAAGATCACTTGTGAAGTGGGTTCTTACGAGGAGATTAACACCCTCGGATCTTGAGGAAGCAGATCTTGATCATGATAAAGTCGTTAGTGCGGCAGAGTTTATCGTATATAAGCTAAAGGAGATGGGGAAGATCAACCAGGAAGATATTGCACTGGTGATGGAAGCGTTCAACAAACTTGACATTGATCATTCAGGAACTTTGACGGCATCTGATTTAATATCAATACCATCTCAACCAACAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005215 GO:0005216 GO:0005242 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006873 GO:0006875 GO:0008150 GO:0008324 GO:0009705 GO:0009987 GO:0010029 GO:0010119 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015276 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0016043 GO:0019725 GO:0022607 GO:0022803 GO:0022832 GO:0022834 GO:0022836 GO:0022838 GO:0022839 GO:0022840 GO:0022841 GO:0022842 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030004 GO:0030007 GO:0030322 GO:0031004 GO:0031090 GO:0031224 GO:0031226 GO:0032991 GO:0034220 GO:0042391 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044425 GO:0044437 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046873 GO:0048580 GO:0048878 GO:0050789 GO:0050793 GO:0050794 GO:0050801 GO:0051179 GO:0051234 GO:0051239 GO:0051259 GO:0051260 GO:0055065 GO:0055067 GO:0055075 GO:0055080 GO:0055082 GO:0055085 GO:0065003 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071840 GO:0071944 GO:0090533 GO:0098533 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098796 GO:0098805 GO:0099094 GO:1900140 GO:1902494 GO:1902495 GO:1904949 GO:1990351 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ion_trans_2

Protein Analysis

362

Amino Acids

40.4

Weight (kDa)

5.63

Isoelectric Point (pI)

30.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans_2 PF07885 82 - 162 1.4e-16 Ion channel
Ion_trans PF00520 100 - 160 6.4e-07 Ion transport protein
Ion_trans_2 PF07885 202 - 276 1.2e-13 Ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014681)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00500 FvH4_3g00500
malus_domestica MD00G1057000.v1.1 MD10G1337300.v1.1
prunus_persica Prupe.4G005900_v2.0.a1 Prupe.4G005900_v2.0.a1 Prupe.4G005900_v2.0.a1
pyrus_communis pycom10g28670
rosa_chinensis RchiOBHm_Chr5g0000671
rosa_laevigata RLG00000030886
rosa_multiflora Rmu_sc0011992.1_g000010
rosa_rugosa Rorug04G0385500
rosa_samantha Rh5AG005600 Rh5BG007200 Rh5CG006000 Rh5DG006000
rosa_wichuraiana Rw5G000570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 202
AccII CGCG 2 cut(s) 153, 528
AciI CCGC 4 cut(s) 151, 170, 200, 929
AclWI GGATC 1 cut(s) 894
AcsI RAATTY 1 cut(s) 533
AfaI GTAC 4 cut(s) 156, 237, 341, 634
AflII CTTAAG 1 cut(s) 554
AgsI TTSAA 3 cut(s) 145, 586, 1009
AjnI CCWGG 1 cut(s) 975
AluBI AGCT 5 cut(s) 409, 515, 550, 743, 952
AluI AGCT 5 cut(s) 409, 515, 550, 743, 952
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 1 cut(s) 560
AlwI GGATC 1 cut(s) 894
AoxI GGCC 2 cut(s) 441, 539
ApeKI GCWGC 2 cut(s) 470, 777
ApoI RAATTY 1 cut(s) 533
AspS9I GGNCC 4 cut(s) 197, 379, 442, 539
AsuHPI GGTGA 1 cut(s) 1006
AvaII GGWCC 2 cut(s) 197, 379
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 482, 789
BccI CCATC 4 cut(s) 145, 955, 991, 1075
BceAI ACGGC 1 cut(s) 1059
BciT130I CCWGG 1 cut(s) 977
BciVI GTATCC 1 cut(s) 762
BclI TGATCA 2 cut(s) 907, 1024
BcoDI GTCTC 1 cut(s) 560
BfaI CTAG 3 cut(s) 410, 650, 710
BfmI CTRYAG 1 cut(s) 186
BfrI CTTAAG 1 cut(s) 554
BfuI GTATCC 1 cut(s) 762
BglII AGATCT 1 cut(s) 901
BisI GCNGC 3 cut(s) 471, 778, 930
BlsI GCNGC 3 cut(s) 472, 779, 931
BmcAI AGTACT 1 cut(s) 237
Bme1390I CCNGG 1 cut(s) 977
Bme18I GGWCC 2 cut(s) 197, 379
BmgT120I GGNCC 4 cut(s) 197, 379, 442, 539
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 1 cut(s) 977
BmsI GCATC 3 cut(s) 304, 664, 1055
BpiI GAAGAC 1 cut(s) 631
BplI GAGNNNNNCTC 2 cut(s) 716, 748
BpuEI CTTGAG 1 cut(s) 911
BsaBI GATNNNNATC 1 cut(s) 906
BsaI GGTCTC 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 882
BsaWI WCCGGW 1 cut(s) 544
Bse1I ACTGG 1 cut(s) 996
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 1 cut(s) 977
BseDI CCNNGG 1 cut(s) 882
BseGI GGATG 1 cut(s) 679
BseJI GATNNNNATC 1 cut(s) 906
BseMII CTCAG 1 cut(s) 764
BseNI ACTGG 1 cut(s) 996
BseRI GAGGAG 1 cut(s) 883
BseXI GCAGC 2 cut(s) 482, 789
Bsh1236I CGCG 2 cut(s) 153, 528
BshFI GGCC 2 cut(s) 443, 541
BsiHKAI GWGCWC 1 cut(s) 365
BsiSI CCGG 1 cut(s) 545
BslFI GGGAC 2 cut(s) 392, 734
BsmAI GTCTC 1 cut(s) 560
BsmFI GGGAC 2 cut(s) 392, 734
BsmI GAATGC 1 cut(s) 325
BsnI GGCC 2 cut(s) 443, 541
Bso31I GGTCTC 1 cut(s) 560
Bsp1286I GDGCHC 1 cut(s) 365
Bsp143I GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
Bsp68I TCGCGA 1 cut(s) 528
BspACI CCGC 4 cut(s) 151, 170, 200, 929
BspANI GGCC 2 cut(s) 443, 541
BspCNI CTCAG 1 cut(s) 765
BspFNI CGCG 2 cut(s) 153, 528
BspHI TCATGA 1 cut(s) 910
BspLI GGNNCC 1 cut(s) 380
BspPI GGATC 1 cut(s) 894
BspTI CTTAAG 1 cut(s) 554
BspTNI GGTCTC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 202
BsrI ACTGG 1 cut(s) 996
BssECI CCNNGG 1 cut(s) 882
BssMI GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
Bst2UI CCWGG 1 cut(s) 977
Bst4CI ACNGT 4 cut(s) 367, 598, 637, 798
BstAFI CTTAAG 1 cut(s) 554
BstAPI GCANNNNNTGC 1 cut(s) 276
BstC8I GCNNGC 1 cut(s) 418
BstDEI CTNAG 1 cut(s) 773
BstF5I GGATG 1 cut(s) 679
BstFNI CGCG 2 cut(s) 153, 528
BstKTI GATC 7 cut(s) 58, 845, 889, 904, 910, 972, 1027
BstMAI GTCTC 1 cut(s) 560
BstMBI GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
BstMWI GCNNNNNNNGC 3 cut(s) 114, 276, 547
BstNI CCWGG 1 cut(s) 977
BstNSI RCATGY 2 cut(s) 400, 420
BstSCI CCNGG 1 cut(s) 975
BstSFI CTRYAG 1 cut(s) 186
BstUI CGCG 2 cut(s) 153, 528
BstV1I GCAGC 2 cut(s) 482, 789
BstV2I GAAGAC 1 cut(s) 631
BstX2I RGATCY 2 cut(s) 886, 901
BstYI RGATCY 2 cut(s) 886, 901
BsuI GTATCC 1 cut(s) 762
BsuRI GGCC 2 cut(s) 443, 541
BtsCI GGATG 1 cut(s) 679
BtsIMutI CAGTG 3 cut(s) 363, 825, 989
BtuMI TCGCGA 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 418
CciI TCATGA 1 cut(s) 910
Cfr13I GGNCC 4 cut(s) 197, 379, 442, 539
Csp6I GTAC 4 cut(s) 155, 236, 340, 633
CviAII CATG 6 cut(s) 132, 337, 397, 417, 439, 911
CviQI GTAC 4 cut(s) 155, 236, 340, 633
DdeI CTNAG 1 cut(s) 773
DpnI GATC 7 cut(s) 57, 844, 888, 903, 909, 971, 1026
DpnII GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
Eco31I GGTCTC 1 cut(s) 560
Eco47I GGWCC 2 cut(s) 197, 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 1 cut(s) 975
FaeI CATG 6 cut(s) 135, 340, 400, 420, 442, 914
FalI AAGNNNNNCTT 4 cut(s) 24, 56, 888, 920
FaqI GGGAC 2 cut(s) 392, 734
FatI CATG 6 cut(s) 131, 336, 396, 416, 438, 910
FbaI TGATCA 2 cut(s) 907, 1024
Fnu4HI GCNGC 3 cut(s) 471, 778, 930
FokI GGATG 1 cut(s) 686
Fsp4HI GCNGC 3 cut(s) 471, 778, 930
FspBI CTAG 3 cut(s) 410, 650, 710
GluI GCNGC 3 cut(s) 471, 778, 930
HaeIII GGCC 2 cut(s) 443, 541
HapII CCGG 1 cut(s) 545
Hin1II CATG 6 cut(s) 135, 340, 400, 420, 442, 914
HinfI GANTC 6 cut(s) 173, 191, 332, 603, 616, 658
HpaII CCGG 1 cut(s) 545
HphI GGTGA 1 cut(s) 1006
Hpy188I TCNGA 5 cut(s) 46, 300, 602, 886, 1051
Hpy188III TCNNGA 9 cut(s) 145, 286, 329, 527, 662, 890, 905, 911, 1032
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 2 cut(s) 617, 828
HpyCH4III ACNGT 4 cut(s) 367, 598, 637, 798
HpyCH4V TGCA 3 cut(s) 254, 416, 989
HpyF10VI GCNNNNNNNGC 3 cut(s) 114, 276, 547
HpyF3I CTNAG 1 cut(s) 773
Hsp92II CATG 6 cut(s) 135, 340, 400, 420, 442, 914
Ksp22I TGATCA 2 cut(s) 907, 1024
Kzo9I GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
LmnI GCTCC 2 cut(s) 547, 740
Lsp1109I GCAGC 2 cut(s) 482, 789
LweI GCATC 3 cut(s) 304, 664, 1055
MaeI CTAG 3 cut(s) 410, 650, 710
MaeIII GTNAC 3 cut(s) 352, 371, 667
MalI GATC 7 cut(s) 57, 844, 888, 903, 909, 971, 1026
MbiI CCGCTC 1 cut(s) 202
MboI GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
MboII GAAGA 5 cut(s) 108, 111, 631, 979, 992
MflI RGATCY 2 cut(s) 886, 901
MhlI GDGCHC 1 cut(s) 365
MluCI AATT 3 cut(s) 218, 456, 533
MlyI GAGTC 1 cut(s) 326
MmeI TCCRAC 1 cut(s) 580
MnlI CCTC 6 cut(s) 260, 285, 553, 861, 886, 892
MseI TTAA 5 cut(s) 27, 555, 875, 1055, 1084
MslI CAYNNNNRTG 1 cut(s) 437
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 1 cut(s) 545
MspR9I CCNGG 1 cut(s) 977
Mva1269I GAATGC 1 cut(s) 325
MvaI CCWGG 1 cut(s) 977
MvnI CGCG 2 cut(s) 153, 528
MwoI GCNNNNNNNGC 3 cut(s) 114, 276, 547
NdeII GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
NlaIII CATG 6 cut(s) 135, 340, 400, 420, 442, 914
NlaIV GGNNCC 1 cut(s) 380
NmuCI GTSAC 1 cut(s) 352
NruI TCGCGA 1 cut(s) 528
NspI RCATGY 2 cut(s) 400, 420
PaeI GCATGC 1 cut(s) 420
PagI TCATGA 1 cut(s) 910
PctI GAATGC 1 cut(s) 325
PfeI GAWTC 5 cut(s) 173, 191, 603, 616, 658
PkrI GCNGC 3 cut(s) 472, 779, 931
PleI GAGTC 1 cut(s) 326
PpsI GAGTC 1 cut(s) 326
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 1 cut(s) 975
PspGI CCWGG 1 cut(s) 975
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 4 cut(s) 197, 379, 442, 539
PspPPI RGGWCCY 1 cut(s) 379
PsuI RGATCY 2 cut(s) 886, 901
RruI TCGCGA 1 cut(s) 528
RsaI GTAC 4 cut(s) 156, 237, 341, 634
RsaNI GTAC 4 cut(s) 155, 236, 340, 633
RseI CAYNNNNRTG 1 cut(s) 437
SaqAI TTAA 5 cut(s) 27, 555, 875, 1055, 1084
SatI GCNGC 3 cut(s) 471, 778, 930
Sau3AI GATC 7 cut(s) 55, 842, 886, 901, 907, 969, 1024
Sau96I GGNCC 4 cut(s) 197, 379, 442, 539
ScaI AGTACT 1 cut(s) 237
SchI GAGTC 1 cut(s) 326
ScrFI CCNGG 1 cut(s) 977
SduI GDGCHC 1 cut(s) 365
SfaNI GCATC 3 cut(s) 304, 664, 1055
SfcI CTRYAG 1 cut(s) 186
SinI GGWCC 2 cut(s) 197, 379
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 554, 890
SmoI CTYRAG 2 cut(s) 554, 890
SphI GCATGC 1 cut(s) 420
Sse9I AATT 3 cut(s) 218, 456, 533
SsiI CCGC 4 cut(s) 151, 170, 200, 929
SspMI CTAG 3 cut(s) 410, 650, 710
StyD4I CCNGG 1 cut(s) 975
TaaI ACNGT 4 cut(s) 367, 598, 637, 798
TaqII GACCGA 1 cut(s) 584
TasI AATT 3 cut(s) 218, 456, 533
TatI WGTACW 2 cut(s) 235, 339
TauI GCSGC 1 cut(s) 932
TfiI GAWTC 5 cut(s) 173, 191, 603, 616, 658
Tru1I TTAA 5 cut(s) 27, 555, 875, 1055, 1084
Tru9I TTAA 5 cut(s) 27, 555, 875, 1055, 1084
TscAI CASTG 3 cut(s) 370, 832, 996
TseFI GTSAC 1 cut(s) 352
TseI GCWGC 2 cut(s) 470, 777
Tsp45I GTSAC 1 cut(s) 352
TspDTI ATGAA 2 cut(s) 180, 421
TspGWI ACGGA 1 cut(s) 686
TspRI CASTG 3 cut(s) 370, 832, 996
Vha464I CTTAAG 1 cut(s) 554
VpaK11BI GGWCC 2 cut(s) 197, 379
XapI RAATTY 1 cut(s) 533
XceI RCATGY 2 cut(s) 400, 420
XspI CTAG 3 cut(s) 410, 650, 710
ZrmI AGTACT 1 cut(s) 237
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.