Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006915

Physical Location & Seq

Genomic Coordinates Forward (+)

11221416 .. 11227152

Gene Structure 5737 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1060700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2277 bp

ATGGCGCCAAATCCAAGGGTGAACGCAGCCTTTAAAGCAATGGCAGAGCTTGGTATTAATGAAAAACAGGTCAAGCCAGTTTTGAAAAATCTCTTGAGATTATTTGACAAAAACTGGGAACTGATTGAAGAAGAGAATTATAGAGTTCTTGTAGATGCTATATTTGATGCGGAGGATACTCAGGGGGTGGAAGAGAAGAAGGAGAAGAAGAAAAGCAAGAACTATGATGAGGAAGATATGGAAGAAGAGCCTCAGCTGCGTCGTGAACCTGCTCGTCCCTCGAAAAGGATACATTCAAGTGGTGATGAGGGTTCATCACAGAAGAAGAAATCTACAAATACTGATCCGGAGGATAATATTGGGGAAGAATTGCCACTGCCTCATCAACCTGAACGTCCCCTGAAAAGGTTGCGAAAAACCCATGGGGGTCAACTTTCACCATCCCCAAGTACCTGCAACCCTATGTTAGGTGGGCCTTTATTGATAAGGCCAAAAGTGGAGAAAGATGAGTTACTTACACGGTCACCTGACTCAAGGGCTGAATTGCATCAACCAGTTTCACCCCATCGTAGTAACAAAAATAAGGGCAAGCAACCTGTTGTGTCTAATCCCGTACCACAGCATGGAAAGCGAATTATTGAGTCAGGCATCGTTCTTTTGCCGAAACATAGGGTTGACAATCATCAATTAATGAAGCCTAAAGATGAGCCCTTCACTGATGACATGGCACAAGATGAGGTTCCTATAGCAGTCATCCTTCCAGATCCCTCAAGTAAAGAAAAACCTCCACTCCAAAATGGTGCAACTGGGGAACAAAATGATCAAGAACCTGTGGCATCCCAGGAGAGAGAAAGTTCAAGAAGTGATATTGTAGCTTCATCTAATGAGAGGAATACGAACTTTGAACTTGCAACCTTAGAAGAGGAATCTTCTAATTTAGAAGTTGTGTCTTCACCCTTAGGAGAGGATGGATCTATAAGTGTATCACCAAATCTTGATGCATTGAAAAAAACTACTGCTTGGGATTCTGTGCATGGAACTAAGGAAACATTATGCATGCAGCCATGTTCTTTGAATGGGCCCGTTGCTGTTACTGCTCATGAAATTCCCAGACTACCCCTGTCTCTGAATGGTGTTGCTGAATGCAGACAAGCAGGTGAGAGGGCTGATTCTAATGGTTTTGCTGATGTTGATAAGGAAGGTGAACTGGAAGCCTCTCGTGGCCTGGTTGTTGTACAACATTGTGATCTACCTCCTGATGATTTAAGGAGTTATCATGACATTGATGACATAACCAAAGGGGAAGAAAGAGTCAAAATACCATGGGTAAATGAAAAAAACAATGAATATCCACCATCCTTCTTCTATATCTCCCGAAGCCTTGTTTTCCAAGATGCTGCTATTAACCTCCGTCTTTCTGGTATTGGGGATGCAAATTGCTGCCCAACTTGCTTTGGTGATTGCCTATCGGCGTCTGTACCTTGTGCTTGTGCATCTCAGACTGAGGGGGATTTTGCTTACACGCGAGAAGGCCTTCTAAAGGATGATTTCCTGGAAGAGTGTATCTCTATGATTCGCAACCCTCAACAGCACCGTCCTTTCTATTGTAAAAGCTGCCCACTTGAAAGAGTTAAGAATGATGATTGTTTAGAACCATGCAAGGGCCACTTAAGGAGGCAATTTATTAAAGAATGCTGGAGCAAGTGTGGCTGCCATAGACAGTGTGGAAATCGAGCGGTCCAGAGAGGCTTAAATTGCAAATTGCAGGTGTTTTTCACTTCTGAAGGAAAAGGATGGGGTCTCCGAACCTTGGAGGACCTGCCAAAAGGTGCCTTTGTGTGTGAGTATGTTGGAGAAGTTTTAACCAGCAAAGAGTTACATGAGAGGAACATTAAAAGCTCCAGAAGTGGCAAACGTCCCTATCCTGTGCGACTGGATGCAAATTGGGCTTCCAAAGCAGATTTGAGGGATAAAGAAGCTCTCTGTTTGGATGCTACAAACTACGGAAATGTTGCTAGGTTTATTAATCACAGATGTCTGGATGCAAACTTGGTTGAAATCCCAGTTGAAGTGGAGACCCCAGATCATTGCTACTATCATCTTGCCTTTTTCACAACAAGAAAAGTAGATGCCTTGGAAGAGCTAACCTGGGATTATGGCATTGACTTCGACGACCATGATCATCCTGTGAAGGTGTTCCAGTGCCAATGTGGCAGCAAATTCTGCAGGAACATGAAACGTTCAAATAGATCTAGATCTGGATCAATTGCAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006304 GO:0006305 GO:0006306 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016458 GO:0019222 GO:0031047 GO:0032259 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043412 GO:0043414 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0044728 GO:0046483 GO:0048519 GO:0050789 GO:0060255 GO:0065007 GO:0071704 GO:0080188 GO:0090304 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pre-SET SET WIYLD

Protein Analysis

759

Amino Acids

85.16

Weight (kDa)

5.72

Isoelectric Point (pI)

53.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WIYLD	PF10440	6 - 59	1.5e-23	Ubiquitin-binding WIYLD domain
Pre-SET	PF05033	433 - 578	5.2e-12	Pre-SET motif
SET	PF00856	598 - 720	1.4e-19	SET domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006915)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g20650 FvH4_2g20650 FvH4_2g20650
malus_domestica	MD00G1060700.v1.1
prunus_persica	Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1 Prupe.8G216300_v2.0.a1
pyrus_communis	pycom05g17400 pycom10g15310
rosa_chinensis	RchiOBHm_Chr6g0286831
rosa_laevigata	RLG00000012533
rosa_multiflora	Rmu_ssc0000279.1_g000009
rosa_roxburghii	Rroxscaffold_7G00181890
rosa_rugosa	Rorug06G0183800 Rorug06G0183900 Rorug06G0183900 Rorug06G0183900
rosa_samantha	Rh6AG295900 Rh6BG300200 Rh6CG308100 Rh6DG291900
rosa_wichuraiana	Rw6G025510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	1145, 1756
Acc36I	ACCTGC	5 cut(s)	277, 461, 1145, 1756, 1827
AccB1I	GGYRCC	2 cut(s)	4, 1829
AccB7I	CCANNNNNTGG	1 cut(s)	623
AccBSI	CCGCTC	1 cut(s)	1736
AccII	CGCG	1 cut(s)	1525
AccIII	TCCGGA	1 cut(s)	346
AciI	CCGC	2 cut(s)	170, 1736
AclI	AACGTT	1 cut(s)	2239
AclWI	GGATC	4 cut(s)	338, 758, 979, 2269
AcsI	RAATTY	2 cut(s)	1104, 2219
AcuI	CTGAAG	1 cut(s)	1803
AcyI	GRCGYC	2 cut(s)	5, 1472
AfaI	GTAC	4 cut(s)	451, 615, 1236, 1479
AfiI	CCNNNNNNNGG	7 cut(s)	285, 405, 467, 623, 1540, 1661, 1810
AflII	CTTAAG	1 cut(s)	1669
AjnI	CCWGG	4 cut(s)	840, 1224, 1551, 2147
AluBI	AGCT	7 cut(s)	49, 256, 875, 1614, 1899, 1979, 2143
AluI	AGCT	7 cut(s)	49, 256, 875, 1614, 1899, 1979, 2143
Alw26I	GTCTC	3 cut(s)	1128, 1805, 2069
AlwI	GGATC	4 cut(s)	338, 758, 979, 2269
Aor13HI	TCCGGA	1 cut(s)	346
AoxI	GGCC	6 cut(s)	473, 488, 1079, 1222, 1531, 1663
ApaI	GGGCCC	1 cut(s)	1083
ApeKI	GCWGC	8 cut(s)	26, 256, 1060, 1397, 1440, 1614, 1710, 2214
ApoI	RAATTY	2 cut(s)	1104, 2219
AseI	ATTAAT	3 cut(s)	57, 689, 2025
Asp700I	GAANNNNTTC	2 cut(s)	1533, 2195
AspLEI	GCGC	1 cut(s)	7
AspS9I	GGNCC	6 cut(s)	473, 1079, 1080, 1663, 1738, 1816
AvaII	GGWCC	2 cut(s)	1738, 1816
AxyI	CCTNAGG	1 cut(s)	958
BaeGI	GKGCMC	1 cut(s)	1083
BanI	GGYRCC	2 cut(s)	4, 1829
BanII	GRGCYC	2 cut(s)	711, 1083
BauI	CACGAG	1 cut(s)	1218
BbsI	GAAGAC	1 cut(s)	942
BbvCI	CCTCAGC	1 cut(s)	252
BbvI	GCAGC	8 cut(s)	38, 243, 1072, 1384, 1427, 1601, 1697, 2226
BccI	CCATC	5 cut(s)	448, 573, 962, 1363, 1788
BcgI	CGANNNNNNTGC	2 cut(s)	2149, 2183
BciT130I	CCWGG	4 cut(s)	842, 1226, 1553, 2149
BciVI	GTATCC	2 cut(s)	169, 282
BclI	TGATCA	2 cut(s)	820, 2179
BcoDI	GTCTC	3 cut(s)	1128, 1805, 2069
BfaI	CTAG	2 cut(s)	2016, 2253
BfmI	CTRYAG	2 cut(s)	744, 2224
BfoI	RGCGCY	1 cut(s)	8
BfrI	CTTAAG	1 cut(s)	1669
BfuAI	ACCTGC	5 cut(s)	277, 461, 1145, 1756, 1827
BfuI	GTATCC	2 cut(s)	169, 282
BglI	GCCNNNNNGGC	1 cut(s)	2211
BglII	AGATCT	2 cut(s)	2249, 2255
BisI	GCNGC	8 cut(s)	27, 257, 1061, 1398, 1441, 1615, 1711, 2215
BlsI	GCNGC	8 cut(s)	28, 258, 1062, 1399, 1442, 1616, 1712, 2216
Bme1390I	CCNGG	4 cut(s)	842, 1226, 1553, 2149
Bme18I	GGWCC	2 cut(s)	1738, 1816
BmgT120I	GGNCC	6 cut(s)	473, 1079, 1080, 1663, 1738, 1816
BmiI	GGNNCC	4 cut(s)	6, 741, 1081, 1831
BmrFI	CCNGG	4 cut(s)	842, 1226, 1553, 2149
BmrI	ACTGGG	3 cut(s)	124, 816, 2057
BmuI	ACTGGG	3 cut(s)	124, 816, 2057
BpiI	GAAGAC	1 cut(s)	942
BplI	GAGNNNNNCTC	2 cut(s)	1550, 1582
BpmI	CTGGAG	2 cut(s)	1717, 1885
Bpu10I	CCTNAGC	1 cut(s)	252
BpuEI	CTTGAG	3 cut(s)	115, 517, 754
BsaBI	GATNNNNATC	2 cut(s)	2254, 2260
BsaHI	GRCGYC	2 cut(s)	5, 1472
BsaI	GGTCTC	2 cut(s)	1805, 2069
BsaJI	CCNNGG	7 cut(s)	14, 421, 840, 1322, 1809, 2133, 2148
BsaWI	WCCGGW	1 cut(s)	346
BsaXI	ACNNNNNCTCC	4 cut(s)	774, 804, 1690, 1720
Bsc4I	CCNNNNNNNGG	7 cut(s)	285, 405, 467, 623, 1540, 1661, 1810
Bse1I	ACTGG	8 cut(s)	77, 119, 554, 811, 1212, 1938, 2063, 2200
Bse21I	CCTNAGG	1 cut(s)	958
Bse3DI	GCAATG	2 cut(s)	45, 2086
Bse8I	GATNNNNATC	2 cut(s)	2254, 2260
BseAI	TCCGGA	1 cut(s)	346
BseBI	CCWGG	4 cut(s)	842, 1226, 1553, 2149
BseDI	CCNNGG	7 cut(s)	14, 421, 840, 1322, 1809, 2133, 2148
BseJI	GATNNNNATC	2 cut(s)	2254, 2260
BseLI	CCNNNNNNNGG	7 cut(s)	285, 405, 467, 623, 1540, 1661, 1810
BseMI	GCAATG	2 cut(s)	45, 2086
BseMII	CTCAG	4 cut(s)	194, 266, 1494, 1511
BseNI	ACTGG	8 cut(s)	77, 119, 554, 811, 1212, 1938, 2063, 2200
BseSI	GKGCMC	1 cut(s)	1083
BseXI	GCAGC	8 cut(s)	38, 243, 1072, 1384, 1427, 1601, 1697, 2226
Bsh1236I	CGCG	1 cut(s)	1525
BshFI	GGCC	6 cut(s)	475, 490, 1081, 1224, 1533, 1665
BshNI	GGYRCC	2 cut(s)	4, 1829
BsiSI	CCGG	1 cut(s)	347
BslFI	GGGAC	3 cut(s)	261, 381, 1902
BslI	CCNNNNNNNGG	7 cut(s)	285, 405, 467, 623, 1540, 1661, 1810
BsmAI	GTCTC	3 cut(s)	1128, 1805, 2069
BsmFI	GGGAC	3 cut(s)	261, 381, 1902
BsmI	GAATGC	2 cut(s)	1148, 1697
BsnI	GGCC	6 cut(s)	475, 490, 1081, 1224, 1533, 1665
Bso31I	GGTCTC	2 cut(s)	1805, 2069
Bsp120I	GGGCCC	1 cut(s)	1079
Bsp1286I	GDGCHC	2 cut(s)	711, 1083
Bsp13I	TCCGGA	1 cut(s)	346
Bsp1407I	TGTACA	1 cut(s)	1234
Bsp19I	CCATGG	2 cut(s)	421, 1322
BspACI	CCGC	2 cut(s)	170, 1736
BspANI	GGCC	6 cut(s)	475, 490, 1081, 1224, 1533, 1665
BspCNI	CTCAG	4 cut(s)	193, 265, 1495, 1510
BspEI	TCCGGA	1 cut(s)	346
BspFNI	CGCG	1 cut(s)	1525
BspHI	TCATGA	2 cut(s)	1099, 1276
BspLI	GGNNCC	4 cut(s)	6, 741, 1081, 1831
BspMAI	CTGCAG	1 cut(s)	2228
BspMI	ACCTGC	5 cut(s)	277, 461, 1145, 1756, 1827
BspPI	GGATC	4 cut(s)	338, 758, 979, 2269
BspQI	GCTCTTC	2 cut(s)	240, 2133
BspT107I	GGYRCC	2 cut(s)	4, 1829
BspTI	CTTAAG	1 cut(s)	1669
BspTNI	GGTCTC	2 cut(s)	1805, 2069
BsrBI	CCGCTC	1 cut(s)	1736
BsrDI	GCAATG	2 cut(s)	45, 2086
BsrGI	TGTACA	1 cut(s)	1234
BsrI	ACTGG	8 cut(s)	77, 119, 554, 811, 1212, 1938, 2063, 2200
BssECI	CCNNGG	7 cut(s)	14, 421, 840, 1322, 1809, 2133, 2148
BssNI	GRCGYC	2 cut(s)	5, 1472
BssSI	CACGAG	1 cut(s)	1218
BssT1I	CCWWGG	5 cut(s)	14, 421, 1322, 1809, 2133
Bst2BI	CACGAG	1 cut(s)	1218
Bst2UI	CCWGG	4 cut(s)	842, 1226, 1553, 2149
Bst4CI	ACNGT	3 cut(s)	522, 1595, 1722
Bst6I	CTCTTC	6 cut(s)	126, 186, 240, 915, 1551, 2133
BstACI	GRCGYC	2 cut(s)	5, 1472
BstAFI	CTTAAG	1 cut(s)	1669
BstAPI	GCANNNNNTGC	1 cut(s)	2223
BstAUI	TGTACA	1 cut(s)	1234
BstC8I	GCNNGC	2 cut(s)	590, 1058
BstDEI	CTNAG	7 cut(s)	180, 252, 916, 958, 1041, 1497, 1503
BstDSI	CCRYGG	2 cut(s)	421, 1322
BstEII	GGTNACC	1 cut(s)	522
BstENI	CCTNNNNNAGG	3 cut(s)	283, 465, 1538
BstFNI	CGCG	1 cut(s)	1525
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	1 cut(s)	7
BstMAI	GTCTC	3 cut(s)	1128, 1805, 2069
BstNI	CCWGG	4 cut(s)	842, 1226, 1553, 2149
BstNSI	RCATGY	1 cut(s)	1060
BstPI	GGTNACC	1 cut(s)	522
BstSCI	CCNGG	4 cut(s)	840, 1224, 1551, 2147
BstSFI	CTRYAG	2 cut(s)	744, 2224
BstSLI	GKGCMC	1 cut(s)	1083
BstUI	CGCG	1 cut(s)	1525
BstV1I	GCAGC	8 cut(s)	38, 243, 1072, 1384, 1427, 1601, 1697, 2226
BstV2I	GAAGAC	1 cut(s)	942
BstX2I	RGATCY	4 cut(s)	763, 971, 2249, 2255
BstYI	RGATCY	4 cut(s)	763, 971, 2249, 2255
Bsu36I	CCTNAGG	1 cut(s)	958
BsuI	GTATCC	2 cut(s)	169, 282
BsuRI	GGCC	6 cut(s)	475, 490, 1081, 1224, 1533, 1665
BtgI	CCRYGG	2 cut(s)	421, 1322
BtsI	GCAGTG	1 cut(s)	374
BtsIMutI	CAGTG	4 cut(s)	374, 714, 1727, 2207
BveI	ACCTGC	5 cut(s)	277, 461, 1145, 1756, 1827
Cac8I	GCNNGC	2 cut(s)	590, 1058
CciI	TCATGA	2 cut(s)	1099, 1276
CfoI	GCGC	1 cut(s)	7
Cfr13I	GGNCC	6 cut(s)	473, 1079, 1080, 1663, 1738, 1816
CseI	GACGC	2 cut(s)	248, 1461
Csp6I	GTAC	4 cut(s)	450, 614, 1235, 1478
CviQI	GTAC	4 cut(s)	450, 614, 1235, 1478
DdeI	CTNAG	7 cut(s)	180, 252, 916, 958, 1041, 1497, 1503
DinI	GGCGCC	1 cut(s)	6
DraI	TTTAAA	1 cut(s)	34
Eam1104I	CTCTTC	6 cut(s)	126, 186, 240, 915, 1551, 2133
EarI	CTCTTC	6 cut(s)	126, 186, 240, 915, 1551, 2133
Eco130I	CCWWGG	5 cut(s)	14, 421, 1322, 1809, 2133
Eco147I	AGGCCT	1 cut(s)	1533
Eco24I	GRGCYC	2 cut(s)	711, 1083
Eco31I	GGTCTC	2 cut(s)	1805, 2069
Eco47I	GGWCC	2 cut(s)	1738, 1816
Eco57I	CTGAAG	1 cut(s)	1803
Eco81I	CCTNAGG	1 cut(s)	958
Eco91I	GGTNACC	1 cut(s)	522
EcoNI	CCTNNNNNAGG	3 cut(s)	283, 465, 1538
EcoO109I	RGGNCCY	1 cut(s)	1816
EcoO65I	GGTNACC	1 cut(s)	522
EcoRII	CCWGG	4 cut(s)	840, 1224, 1551, 2147
EcoT14I	CCWWGG	5 cut(s)	14, 421, 1322, 1809, 2133
EcoT22I	ATGCAT	2 cut(s)	1003, 1058
EcoT38I	GRGCYC	2 cut(s)	711, 1083
EgeI	GGCGCC	1 cut(s)	6
EheI	GGCGCC	1 cut(s)	6
ErhI	CCWWGG	5 cut(s)	14, 421, 1322, 1809, 2133
FalI	AAGNNNNNCTT	2 cut(s)	1652, 1684
FaqI	GGGAC	3 cut(s)	261, 381, 1902
FbaI	TGATCA	2 cut(s)	820, 2179
Fnu4HI	GCNGC	8 cut(s)	27, 257, 1061, 1398, 1441, 1615, 1711, 2215
FriOI	GRGCYC	2 cut(s)	711, 1083
Fsp4HI	GCNGC	8 cut(s)	27, 257, 1061, 1398, 1441, 1615, 1711, 2215
FspBI	CTAG	2 cut(s)	2016, 2253
GlaI	GCGC	1 cut(s)	6
GluI	GCNGC	8 cut(s)	27, 257, 1061, 1398, 1441, 1615, 1711, 2215
GsuI	CTGGAG	2 cut(s)	1717, 1885
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	6 cut(s)	475, 490, 1081, 1224, 1533, 1665
HapII	CCGG	1 cut(s)	347
HgaI	GACGC	2 cut(s)	248, 1461
HhaI	GCGC	1 cut(s)	7
Hin1I	GRCGYC	2 cut(s)	5, 1472
Hin6I	GCGC	1 cut(s)	5
HinP1I	GCGC	1 cut(s)	5
HincII	GTYRAC	2 cut(s)	431, 676
HindII	GTYRAC	2 cut(s)	431, 676
HinfI	GANTC	7 cut(s)	530, 641, 926, 1025, 1169, 1311, 1573
HpaII	CCGG	1 cut(s)	347
Hpy166II	GTNNAC	5 cut(s)	22, 266, 431, 676, 1205
Hpy188I	TCNGA	4 cut(s)	1128, 1500, 1783, 1805
Hpy8I	GTNNAC	5 cut(s)	22, 266, 431, 676, 1205
Hpy99I	CGWCG	2 cut(s)	264, 2174
HpyAV	CCTTC	9 cut(s)	193, 721, 767, 1193, 1369, 1523, 1544, 1778, 2185
HpyCH4III	ACNGT	3 cut(s)	522, 1595, 1722
HpyCH4IV	ACGT	3 cut(s)	394, 1915, 2239
HpyF3I	CTNAG	7 cut(s)	180, 252, 916, 958, 1041, 1497, 1503
HpySE526I	ACGT	3 cut(s)	394, 1915, 2239
Hsp92I	GRCGYC	2 cut(s)	5, 1472
HspAI	GCGC	1 cut(s)	5
KasI	GGCGCC	1 cut(s)	4
Kpn2I	TCCGGA	1 cut(s)	346
Ksp22I	TGATCA	2 cut(s)	820, 2179
LguI	GCTCTTC	2 cut(s)	240, 2133
LmnI	GCTCC	2 cut(s)	1698, 1904
Lsp1109I	GCAGC	8 cut(s)	38, 243, 1072, 1384, 1427, 1601, 1697, 2226
MaeI	CTAG	2 cut(s)	2016, 2253
MaeII	ACGT	3 cut(s)	394, 1915, 2239
MaeIII	GTNAC	5 cut(s)	510, 522, 572, 1090, 1875
MbiI	CCGCTC	1 cut(s)	1736
MfeI	CAATTG	1 cut(s)	2265
MflI	RGATCY	4 cut(s)	763, 971, 2249, 2255
MhlI	GDGCHC	2 cut(s)	711, 1083
Mly113I	GGCGCC	1 cut(s)	5
MlyI	GAGTC	3 cut(s)	524, 650, 1320
MmeI	TCCRAC	1 cut(s)	1831
Mph1103I	ATGCAT	2 cut(s)	1003, 1058
MroI	TCCGGA	1 cut(s)	346
MroXI	GAANNNNTTC	2 cut(s)	1533, 2195
MslI	CAYNNNNRTG	1 cut(s)	297
MspA1I	CMGCKG	1 cut(s)	256
MspCI	CTTAAG	1 cut(s)	1669
MspI	CCGG	1 cut(s)	347
MspR9I	CCNGG	4 cut(s)	842, 1226, 1553, 2149
MunI	CAATTG	1 cut(s)	2265
Mva1269I	GAATGC	2 cut(s)	1148, 1697
MvaI	CCWGG	4 cut(s)	842, 1226, 1553, 2149
MvnI	CGCG	1 cut(s)	1525
NarI	GGCGCC	1 cut(s)	5
NcoI	CCATGG	2 cut(s)	421, 1322
NlaIV	GGNNCC	4 cut(s)	6, 741, 1081, 1831
NmuCI	GTSAC	1 cut(s)	522
NsiI	ATGCAT	2 cut(s)	1003, 1058
NspI	RCATGY	1 cut(s)	1060
PaeI	GCATGC	1 cut(s)	1060
PagI	TCATGA	2 cut(s)	1099, 1276
PaqCI	CACCTGC	2 cut(s)	1145, 1756
PceI	AGGCCT	1 cut(s)	1533
PciSI	GCTCTTC	2 cut(s)	240, 2133
PctI	GAATGC	2 cut(s)	1148, 1697
PdmI	GAANNNNTTC	2 cut(s)	1533, 2195
PfeI	GAWTC	4 cut(s)	926, 1025, 1169, 1573
PflMI	CCANNNNNTGG	1 cut(s)	623
PfoI	TCCNGGA	1 cut(s)	1551
PkrI	GCNGC	8 cut(s)	28, 258, 1062, 1399, 1442, 1616, 1712, 2216
PleI	GAGTC	3 cut(s)	524, 649, 1319
PluTI	GGCGCC	1 cut(s)	8
PpsI	GAGTC	3 cut(s)	524, 649, 1319
PpuMI	RGGWCCY	1 cut(s)	1816
PshBI	ATTAAT	3 cut(s)	57, 689, 2025
Psp1406I	AACGTT	1 cut(s)	2239
Psp5II	RGGWCCY	1 cut(s)	1816
Psp6I	CCWGG	4 cut(s)	840, 1224, 1551, 2147
PspEI	GGTNACC	1 cut(s)	522
PspGI	CCWGG	4 cut(s)	840, 1224, 1551, 2147
PspN4I	GGNNCC	4 cut(s)	6, 741, 1081, 1831
PspOMI	GGGCCC	1 cut(s)	1079
PspPI	GGNCC	6 cut(s)	473, 1079, 1080, 1663, 1738, 1816
PspPPI	RGGWCCY	1 cut(s)	1816
PstI	CTGCAG	1 cut(s)	2228
PsuI	RGATCY	4 cut(s)	763, 971, 2249, 2255
PvuII	CAGCTG	1 cut(s)	256
RsaI	GTAC	4 cut(s)	451, 615, 1236, 1479
RsaNI	GTAC	4 cut(s)	450, 614, 1235, 1478
RseI	CAYNNNNRTG	1 cut(s)	297
SapI	GCTCTTC	2 cut(s)	240, 2133
SatI	GCNGC	8 cut(s)	27, 257, 1061, 1398, 1441, 1615, 1711, 2215
Sau96I	GGNCC	6 cut(s)	473, 1079, 1080, 1663, 1738, 1816
SchI	GAGTC	3 cut(s)	524, 650, 1320
ScrFI	CCNGG	4 cut(s)	842, 1226, 1553, 2149
SduI	GDGCHC	2 cut(s)	711, 1083
SfcI	CTRYAG	2 cut(s)	744, 2224
SfoI	GGCGCC	1 cut(s)	6
SinI	GGWCC	2 cut(s)	1738, 1816
SmiMI	CAYNNNNRTG	1 cut(s)	297
SmlI	CTYRAG	4 cut(s)	94, 532, 769, 1669
SmoI	CTYRAG	4 cut(s)	94, 532, 769, 1669
SphI	GCATGC	1 cut(s)	1060
SseBI	AGGCCT	1 cut(s)	1533
SsiI	CCGC	2 cut(s)	170, 1736
SspDI	GGCGCC	1 cut(s)	4
SspI	AATATT	1 cut(s)	358
SspMI	CTAG	2 cut(s)	2016, 2253
StuI	AGGCCT	1 cut(s)	1533
StyD4I	CCNGG	4 cut(s)	840, 1224, 1551, 2147
StyI	CCWWGG	5 cut(s)	14, 421, 1322, 1809, 2133
TaaI	ACNGT	3 cut(s)	522, 1595, 1722
TaiI	ACGT	3 cut(s)	397, 1918, 2242
TaqI	TCGA	3 cut(s)	281, 1732, 2169
TatI	WGTACW	1 cut(s)	1234
TfiI	GAWTC	4 cut(s)	926, 1025, 1169, 1573
TscAI	CASTG	4 cut(s)	381, 721, 1727, 2207
TseFI	GTSAC	1 cut(s)	522
TseI	GCWGC	8 cut(s)	26, 256, 1060, 1397, 1440, 1614, 1710, 2214
Tsp45I	GTSAC	1 cut(s)	522
TspDTI	ATGAA	8 cut(s)	75, 303, 707, 867, 1116, 1347, 1359, 2249
TspGWI	ACGGA	2 cut(s)	1400, 2019
TspRI	CASTG	4 cut(s)	381, 721, 1727, 2207
Van91I	CCANNNNNTGG	1 cut(s)	623
Vha464I	CTTAAG	1 cut(s)	1669
VpaK11BI	GGWCC	2 cut(s)	1738, 1816
VspI	ATTAAT	3 cut(s)	57, 689, 2025
XagI	CCTNNNNNAGG	3 cut(s)	283, 465, 1538
XapI	RAATTY	2 cut(s)	1104, 2219
XbaI	TCTAGA	1 cut(s)	2252
XceI	RCATGY	1 cut(s)	1060
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1721, 2207
XmnI	GAANNNNTTC	2 cut(s)	1533, 2195
XspI	CTAG	2 cut(s)	2016, 2253
Zsp2I	ATGCAT	2 cut(s)	1003, 1058

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.