MD00G1080900.v1.1

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0011117
Physical Location & Seq
Forward (+)
16178443 .. 16182228
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1080900.v1.1.491

Sequence Viewer

Length: 1548 bp
ATGGAGGTTAGATCTGAAGTCGGTTTACATGTGCGGTGCGAGAAGATTCCGGCGCCGGTGATTGGGCGGACGCGGTTGCAGGTGTGGTTCATTCGGGTGTGTTCGAGCTTGTTCCTGTGGACGTGTTTGGTTCAGTTAGTGGCGGTTGGGGAGCTATGGCATCCGCATTTGCTCTCCAATATAACCCATCGGATATCCGAAATCACGCTGGTTCCACTTCCGGCGGTCCCATCTCCGCCGCCTCTTCTCCCCGCCAATAATTTTGCTGGTGTTACTTCTATTGAGCATGAATGTGATTGTTGGAGAAATTATACAAGTAACGGGTTTCTTAGAGTTTCCTGCAATGGGGGCTTGAATCAAATGCGTGCTGCGATTTGTGACATGGTCACTGTTGCCCGGCTTTTAAATCTTACATTGGTTGTTCCAGAGCTTGATAAGACATCTTTCTGGGCTGATCCTAGTAACTTTGAGGATATCTTTGATCTAAGACATTTCATTAATTCACTGCGAGATGAAGTCCGAATTGTCAGAAGACTTCCAAAAATGTTTAGTAGAAAATATGGACATAAACCGCTCGAGATGCCTCCTATTAGTTGGTCAAATGAAAAATACTATCTTGAACAGATATTGCCACTTGTTAGCAAGCATAAAGTGTTGCACTTTAACAGAACAGATGCACGTTTGGCAAACAATTGGATACCTCTTGATCTTCAGAAACTTCGGTGTCGTGTTAATTTCCAAGCACTGAAGTTCACTCCCGAGATTGAGGCTTTAGGGTACAAATTGGTTCGTCTACTTAAAGAAAAGGGACCTTATGTGGCTTTGCATCTAAGGTACGAAATGGACATGTTGGCTTTCTCAGGTTGTACTCATGGCTGCACTGAGGAAGAAGCTGATGAGCTCAAACGATTGAGATATGCATATCCTTGGTGGAGAGAGAAAGAGATAATGTCTGAAGAGAAGAGATCTCAAGGTTTGTGTCCTCTAACGCCCGAGGAGAGTGCATTAATATTGCAAGCCTTGGGGTTTAGTAAGGACACACAAATCTATATAGCAGCTGGTGAGATTTATGGTAGTGAAAGGAGACTTGGTCCACTACGAGCTGCATTTCCACGGATTGTTAAGAAGGAAACACTTCTTGCTCCGAAGGAGTTGCGGCAGTTTCAGAATCATTCATCTCAAATGGCAGCTCTGGATTTTATAGTTTCGGTTGCTAGTAATACTTTTGTTCCTACATATGATGGGAATATGGCGAAACTTGTTGAAGGTCACCGAAGGTACCATGGGTTTAAAAAAAGCATCTTGCTCGACAGGAAAAGACTTGTAGAATTATTGGACTTGCATCTGAATGGGACACATTCGTGGAATCAGTTTTCGGACGCTGTACGTTCCATTCATGAGAAAAGAATGGGACAACCAACTCGGCGAAGAGTCATCGCAGACAGGCCAAAGGAGGAAGATTATTTCTACGCAAATCCTCATGAATGCCTCTGCGAGGAAACGAATTGTGATGACTTTAGCCCTGGAAACTCAAGCAAAACCGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

516

Amino Acids

59.35

Weight (kDa)

8.73

Isoelectric Point (pI)

55.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 109 - 428 2.2e-108 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14020 AT2G03280
fragaria_vesca FvH4_4g30810
malus_domestica MD00G1080900.v1.1 MD16G1067800.v1.1
prunus_persica Prupe.1G279000_v2.0.a1
pyrus_communis pycom13g06080 pycom16g05930 pycom16g05940
rosa_chinensis RchiOBHm_Chr4g0439201
rosa_laevigata RLG00000006288
rosa_multiflora Rmu_sc0002236.1_g000012
rosa_roxburghii Rroxscaffold_5G00380190
rosa_rugosa Rorug04G0317000
rosa_samantha Rh4AG367500 Rh4CG393500 Rh4DG373700
rosa_wichuraiana Rw0G005660 Rw0G011650 Rw5G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 70
Acc36I ACCTGC 1 cut(s) 70
Acc65I GGTACC 1 cut(s) 1278
AccB1I GGYRCC 2 cut(s) 52, 1278
AccBSI CCGCTC 1 cut(s) 574
AccI GTMKAC 1 cut(s) 793
AccII CGCG 1 cut(s) 73
AclWI GGATC 1 cut(s) 449
AcuI CTGAAG 4 cut(s) 36, 695, 767, 975
AcyI GRCGYC 1 cut(s) 53
AfaI GTAC 5 cut(s) 779, 836, 868, 1280, 1386
AfiI CCNNNNNNNGG 3 cut(s) 62, 345, 1495
AflIII ACRYGT 3 cut(s) 28, 122, 846
AgsI TTSAA 3 cut(s) 355, 620, 1265
AjiI CACGTC 1 cut(s) 123
AjnI CCWGG 1 cut(s) 1522
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
AleI CACNNNNGTG 1 cut(s) 1360
AluBI AGCT 8 cut(s) 108, 154, 430, 893, 901, 1058, 1103, 1190
AluI AGCT 8 cut(s) 108, 154, 430, 893, 901, 1058, 1103, 1190
Alw21I GWGCWC 1 cut(s) 903
Alw26I GTCTC 1 cut(s) 1078
AlwI GGATC 1 cut(s) 449
Ama87I CYCGRG 3 cut(s) 575, 758, 992
AoxI GGCC 1 cut(s) 1445
ApeKI GCWGC 5 cut(s) 368, 876, 1055, 1103, 1187
AseI ATTAAT 2 cut(s) 498, 1007
Asp700I GAANNNNTTC 1 cut(s) 1134
Asp718I GGTACC 1 cut(s) 1278
AspLEI GCGC 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 226, 809, 1091
AsuC2I CCSGG 2 cut(s) 397, 1543
AsuHPI GGTGA 3 cut(s) 70, 1073, 1262
AvaI CYCGRG 3 cut(s) 575, 758, 992
AvaII GGWCC 3 cut(s) 226, 809, 1091
BanI GGYRCC 2 cut(s) 52, 1278
BanII GRGCYC 1 cut(s) 903
BarI GAAGNNNNNNTAC 2 cut(s) 9, 41
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 903
BbvI GCAGC 5 cut(s) 355, 863, 1067, 1090, 1199
BccI CCATC 3 cut(s) 195, 238, 1235
BcgI CGANNNNNNTGC 6 cut(s) 983, 1017, 1135, 1169, 1288, 1322
BciT130I CCWGG 1 cut(s) 1524
BciVI GTATCC 1 cut(s) 690
BcnI CCSGG 2 cut(s) 397, 1543
BcoDI GTCTC 1 cut(s) 1078
BfaI CTAG 2 cut(s) 459, 1215
BfoI RGCGCY 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 70
BfuI GTATCC 1 cut(s) 690
BglII AGATCT 2 cut(s) 11, 965
BisI GCNGC 7 cut(s) 239, 369, 877, 1056, 1104, 1157, 1188
BlsI GCNGC 7 cut(s) 240, 370, 878, 1057, 1105, 1158, 1189
Bme1390I CCNGG 3 cut(s) 397, 1524, 1543
Bme18I GGWCC 3 cut(s) 226, 809, 1091
BmeT110I CYCGRG 3 cut(s) 575, 758, 992
BmgBI CACGTC 1 cut(s) 123
BmgT120I GGNCC 3 cut(s) 226, 809, 1091
BmiI GGNNCC 5 cut(s) 54, 213, 228, 810, 1280
BmrFI CCNGG 3 cut(s) 397, 1524, 1543
BmsI GCATC 6 cut(s) 169, 570, 664, 835, 1308, 1351
BpiI GAAGAC 1 cut(s) 538
BpuEI CTTGAG 2 cut(s) 954, 1516
BpuMI CCSGG 2 cut(s) 397, 1543
BsaHI GRCGYC 1 cut(s) 53
BsaJI CCNNGG 6 cut(s) 926, 993, 1020, 1112, 1282, 1522
BsaXI ACNNNNNCTCC 2 cut(s) 1075, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 345, 1495
Bse118I RCCGGY 1 cut(s) 55
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 1524
BseDI CCNNGG 6 cut(s) 926, 993, 1020, 1112, 1282, 1522
BseGI GGATG 1 cut(s) 160
BseLI CCNNNNNNNGG 3 cut(s) 62, 345, 1495
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 2 cut(s) 873, 873
BseRI GAGGAG 1 cut(s) 1010
BseXI GCAGC 5 cut(s) 355, 863, 1067, 1090, 1199
BsgI GTGCAG 1 cut(s) 862
Bsh1236I CGCG 1 cut(s) 73
BshFI GGCC 1 cut(s) 1447
BshNI GGYRCC 2 cut(s) 52, 1278
BsiHKAI GWGCWC 1 cut(s) 903
BsiHKCI CYCGRG 3 cut(s) 575, 758, 992
BsiSI CCGG 5 cut(s) 50, 56, 221, 397, 1542
BslFI GGGAC 4 cut(s) 212, 822, 1366, 1425
BslI CCNNNNNNNGG 3 cut(s) 62, 345, 1495
BsmAI GTCTC 1 cut(s) 1078
BsmFI GGGAC 4 cut(s) 212, 822, 1366, 1425
BsmI GAATGC 1 cut(s) 1490
BsnI GGCC 1 cut(s) 1447
BsoBI CYCGRG 3 cut(s) 575, 758, 992
Bsp1286I GDGCHC 1 cut(s) 903
Bsp143I GATC 5 cut(s) 11, 454, 481, 706, 965
Bsp19I CCATGG 1 cut(s) 1282
BspANI GGCC 1 cut(s) 1447
BspCNI CTCAG 2 cut(s) 872, 874
BspFNI CGCG 1 cut(s) 73
BspHI TCATGA 2 cut(s) 1396, 1480
BspLI GGNNCC 5 cut(s) 54, 213, 228, 810, 1280
BspMI ACCTGC 1 cut(s) 70
BspPI GGATC 1 cut(s) 449
BspT107I GGYRCC 2 cut(s) 52, 1278
BsrBI CCGCTC 1 cut(s) 574
BsrDI GCAATG 1 cut(s) 349
BsrFI RCCGGY 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 55
BssECI CCNNGG 6 cut(s) 926, 993, 1020, 1112, 1282, 1522
BssMI GATC 5 cut(s) 11, 454, 481, 706, 965
BssNI GRCGYC 1 cut(s) 53
BssT1I CCWWGG 3 cut(s) 926, 1020, 1282
Bst2UI CCWGG 1 cut(s) 1524
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 4 cut(s) 249, 951, 956, 1423
BstACI GRCGYC 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 366, 644, 1017
BstDEI CTNAG 5 cut(s) 329, 485, 830, 859, 882
BstDSI CCRYGG 2 cut(s) 1112, 1282
BstEII GGTNACC 1 cut(s) 1268
BstENI CCTNNNNNAGG 1 cut(s) 1493
BstF5I GGATG 1 cut(s) 160
BstFNI CGCG 1 cut(s) 73
BstH2I RGCGCY 1 cut(s) 56
BstHHI GCGC 1 cut(s) 55
BstKTI GATC 5 cut(s) 14, 457, 484, 709, 968
BstMAI GTCTC 1 cut(s) 1078
BstMBI GATC 5 cut(s) 11, 454, 481, 706, 965
BstMWI GCNNNNNNNGC 3 cut(s) 348, 580, 683
BstNI CCWGG 1 cut(s) 1524
BstNSI RCATGY 2 cut(s) 32, 850
BstPI GGTNACC 1 cut(s) 1268
BstSCI CCNGG 3 cut(s) 395, 1522, 1541
BstUI CGCG 1 cut(s) 73
BstV1I GCAGC 5 cut(s) 355, 863, 1067, 1090, 1199
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 2 cut(s) 11, 965
BstYI RGATCY 2 cut(s) 11, 965
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 1 cut(s) 1447
BtgI CCRYGG 2 cut(s) 1112, 1282
BtgZI GCGATG 1 cut(s) 1420
BtrI CACGTC 1 cut(s) 123
BtsCI GGATG 1 cut(s) 160
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 4 cut(s) 387, 503, 743, 879
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 3 cut(s) 366, 644, 1017
CciI TCATGA 2 cut(s) 1396, 1480
CfoI GCGC 1 cut(s) 55
Cfr10I RCCGGY 1 cut(s) 55
Cfr13I GGNCC 3 cut(s) 226, 809, 1091
CseI GACGC 2 cut(s) 79, 1388
Csp6I GTAC 5 cut(s) 778, 835, 867, 1279, 1385
CviAII CATG 8 cut(s) 29, 287, 382, 847, 872, 1283, 1397, 1481
CviQI GTAC 5 cut(s) 778, 835, 867, 1279, 1385
DdeI CTNAG 5 cut(s) 329, 485, 830, 859, 882
DinI GGCGCC 1 cut(s) 54
DpnI GATC 5 cut(s) 13, 456, 483, 708, 967
DpnII GATC 5 cut(s) 11, 454, 481, 706, 965
DraI TTTAAA 2 cut(s) 405, 1291
Eam1104I CTCTTC 4 cut(s) 249, 951, 956, 1423
EarI CTCTTC 4 cut(s) 249, 951, 956, 1423
EciI GGCGGA 2 cut(s) 82, 225
Ecl136II GAGCTC 1 cut(s) 901
Eco130I CCWWGG 3 cut(s) 926, 1020, 1282
Eco24I GRGCYC 1 cut(s) 903
Eco32I GATATC 2 cut(s) 195, 475
Eco47I GGWCC 3 cut(s) 226, 809, 1091
Eco53kI GAGCTC 1 cut(s) 901
Eco57I CTGAAG 4 cut(s) 36, 695, 767, 975
Eco88I CYCGRG 3 cut(s) 575, 758, 992
Eco91I GGTNACC 1 cut(s) 1268
EcoICRI GAGCTC 1 cut(s) 901
EcoNI CCTNNNNNAGG 1 cut(s) 1493
EcoO109I RGGNCCY 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 1268
EcoRII CCWGG 1 cut(s) 1522
EcoRV GATATC 2 cut(s) 195, 475
EcoT14I CCWWGG 3 cut(s) 926, 1020, 1282
EcoT22I ATGCAT 1 cut(s) 922
EcoT38I GRGCYC 1 cut(s) 903
EgeI GGCGCC 1 cut(s) 54
EheI GGCGCC 1 cut(s) 54
ErhI CCWWGG 3 cut(s) 926, 1020, 1282
FaeI CATG 8 cut(s) 32, 290, 385, 850, 875, 1286, 1400, 1484
FaqI GGGAC 4 cut(s) 212, 822, 1366, 1425
FatI CATG 8 cut(s) 28, 286, 381, 846, 871, 1282, 1396, 1480
FauI CCCGC 1 cut(s) 259
FauNDI CATATG 1 cut(s) 1237
FblI GTMKAC 1 cut(s) 793
Fnu4HI GCNGC 7 cut(s) 239, 369, 877, 1056, 1104, 1157, 1188
FokI GGATG 1 cut(s) 147
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 7 cut(s) 239, 369, 877, 1056, 1104, 1157, 1188
FspBI CTAG 2 cut(s) 459, 1215
GlaI GCGC 1 cut(s) 54
GluI GCNGC 7 cut(s) 239, 369, 877, 1056, 1104, 1157, 1188
HaeII RGCGCY 1 cut(s) 56
HaeIII GGCC 1 cut(s) 1447
HapII CCGG 5 cut(s) 50, 56, 221, 397, 1542
HgaI GACGC 2 cut(s) 79, 1388
HhaI GCGC 1 cut(s) 55
Hin1I GRCGYC 1 cut(s) 53
Hin1II CATG 8 cut(s) 32, 290, 385, 850, 875, 1286, 1400, 1484
Hin6I GCGC 1 cut(s) 53
HinP1I GCGC 1 cut(s) 53
HinfI GANTC 5 cut(s) 46, 355, 1168, 1366, 1431
HpaII CCGG 5 cut(s) 50, 56, 221, 397, 1542
HphI GGTGA 3 cut(s) 70, 1073, 1262
Hpy166II GTNNAC 5 cut(s) 26, 120, 753, 794, 1094
Hpy188III TCNNGA 8 cut(s) 425, 577, 617, 704, 758, 1193, 1397, 1481
Hpy8I GTNNAC 5 cut(s) 26, 120, 753, 794, 1094
HpyAV CCTTC 4 cut(s) 1120, 1141, 1259, 1269
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 3 cut(s) 122, 679, 1387
HpyF10VI GCNNNNNNNGC 3 cut(s) 348, 580, 683
HpyF3I CTNAG 5 cut(s) 329, 485, 830, 859, 882
HpySE526I ACGT 3 cut(s) 122, 679, 1387
Hsp92I GRCGYC 1 cut(s) 53
Hsp92II CATG 8 cut(s) 32, 290, 385, 850, 875, 1286, 1400, 1484
HspAI GCGC 1 cut(s) 53
KasI GGCGCC 1 cut(s) 52
KpnI GGTACC 1 cut(s) 1282
Kzo9I GATC 5 cut(s) 11, 454, 481, 706, 965
LmnI GCTCC 2 cut(s) 151, 1147
Lsp1109I GCAGC 5 cut(s) 355, 863, 1067, 1090, 1199
LweI GCATC 6 cut(s) 169, 570, 664, 835, 1308, 1351
MaeI CTAG 2 cut(s) 459, 1215
MaeII ACGT 3 cut(s) 122, 679, 1387
MaeIII GTNAC 6 cut(s) 271, 317, 377, 385, 461, 1268
MalI GATC 5 cut(s) 13, 456, 483, 708, 967
MbiI CCGCTC 1 cut(s) 574
MboI GATC 5 cut(s) 11, 454, 481, 706, 965
MboII GAAGA 9 cut(s) 55, 236, 543, 701, 899, 968, 973, 1440, 1469
MfeI CAATTG 1 cut(s) 691
MflI RGATCY 2 cut(s) 11, 965
MhlI GDGCHC 1 cut(s) 903
MluCI AATT 9 cut(s) 259, 307, 499, 522, 691, 733, 782, 1328, 1504
Mly113I GGCGCC 1 cut(s) 53
MlyI GAGTC 1 cut(s) 1440
MmeI TCCRAC 1 cut(s) 281
Mph1103I ATGCAT 1 cut(s) 922
MroXI GAANNNNTTC 1 cut(s) 1134
MseI TTAA 8 cut(s) 404, 498, 663, 732, 798, 1007, 1122, 1290
MslI CAYNNNNRTG 4 cut(s) 95, 291, 1347, 1360
MspA1I CMGCKG 1 cut(s) 1058
MspI CCGG 5 cut(s) 50, 56, 221, 397, 1542
MspR9I CCNGG 3 cut(s) 397, 1524, 1543
MunI CAATTG 1 cut(s) 691
Mva1269I GAATGC 1 cut(s) 1490
MvaI CCWGG 1 cut(s) 1524
MvnI CGCG 1 cut(s) 73
MwoI GCNNNNNNNGC 3 cut(s) 348, 580, 683
NarI GGCGCC 1 cut(s) 53
NciI CCSGG 2 cut(s) 397, 1543
NcoI CCATGG 1 cut(s) 1282
NdeI CATATG 1 cut(s) 1237
NdeII GATC 5 cut(s) 11, 454, 481, 706, 965
NlaIII CATG 8 cut(s) 32, 290, 385, 850, 875, 1286, 1400, 1484
NlaIV GGNNCC 5 cut(s) 54, 213, 228, 810, 1280
NmeAIII GCCGAG 1 cut(s) 1402
NmuCI GTSAC 3 cut(s) 377, 385, 1268
NsiI ATGCAT 1 cut(s) 922
NspI RCATGY 2 cut(s) 32, 850
OliI CACNNNNGTG 1 cut(s) 1360
PaeR7I CTCGAG 1 cut(s) 575
PagI TCATGA 2 cut(s) 1396, 1480
PaqCI CACCTGC 1 cut(s) 70
PciI ACATGT 2 cut(s) 28, 846
PctI GAATGC 1 cut(s) 1490
PdmI GAANNNNTTC 1 cut(s) 1134
PfeI GAWTC 4 cut(s) 46, 355, 1168, 1366
PflFI GACNNNGTC 2 cut(s) 383, 1089
PkrI GCNGC 7 cut(s) 240, 370, 878, 1057, 1105, 1158, 1189
PleI GAGTC 1 cut(s) 1439
PluTI GGCGCC 1 cut(s) 56
PpsI GAGTC 1 cut(s) 1439
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 2 cut(s) 28, 846
PshBI ATTAAT 2 cut(s) 498, 1007
Psp124BI GAGCTC 1 cut(s) 903
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 1 cut(s) 1522
PspEI GGTNACC 1 cut(s) 1268
PspGI CCWGG 1 cut(s) 1522
PspN4I GGNNCC 5 cut(s) 54, 213, 228, 810, 1280
PspPI GGNCC 3 cut(s) 226, 809, 1091
PspPPI RGGWCCY 1 cut(s) 809
PsuI RGATCY 2 cut(s) 11, 965
PsyI GACNNNGTC 2 cut(s) 383, 1089
PvuII CAGCTG 1 cut(s) 1058
RsaI GTAC 5 cut(s) 779, 836, 868, 1280, 1386
RsaNI GTAC 5 cut(s) 778, 835, 867, 1279, 1385
RseI CAYNNNNRTG 4 cut(s) 95, 291, 1347, 1360
SacI GAGCTC 1 cut(s) 903
SaqAI TTAA 8 cut(s) 404, 498, 663, 732, 798, 1007, 1122, 1290
SatI GCNGC 7 cut(s) 239, 369, 877, 1056, 1104, 1157, 1188
Sau3AI GATC 5 cut(s) 11, 454, 481, 706, 965
Sau96I GGNCC 3 cut(s) 226, 809, 1091
SchI GAGTC 1 cut(s) 1440
ScrFI CCNGG 3 cut(s) 397, 1524, 1543
SduI GDGCHC 1 cut(s) 903
SfaNI GCATC 6 cut(s) 169, 570, 664, 835, 1308, 1351
SfoI GGCGCC 1 cut(s) 54
Sfr274I CTCGAG 1 cut(s) 575
SgrAI CRCCGGYG 1 cut(s) 55
SinI GGWCC 3 cut(s) 226, 809, 1091
SlaI CTCGAG 1 cut(s) 575
SmiMI CAYNNNNRTG 4 cut(s) 95, 291, 1347, 1360
SmlI CTYRAG 3 cut(s) 575, 969, 1531
SmoI CTYRAG 3 cut(s) 575, 969, 1531
Sse9I AATT 9 cut(s) 259, 307, 499, 522, 691, 733, 782, 1328, 1504
SspDI GGCGCC 1 cut(s) 52
SspI AATATT 1 cut(s) 1011
SspMI CTAG 2 cut(s) 459, 1215
SstI GAGCTC 1 cut(s) 903
StyD4I CCNGG 3 cut(s) 395, 1522, 1541
StyI CCWWGG 3 cut(s) 926, 1020, 1282
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 3 cut(s) 125, 682, 1390
TaqI TCGA 3 cut(s) 104, 576, 1308
TasI AATT 9 cut(s) 259, 307, 499, 522, 691, 733, 782, 1328, 1504
TatI WGTACW 1 cut(s) 866
TauI GCSGC 2 cut(s) 241, 1159
TfiI GAWTC 4 cut(s) 46, 355, 1168, 1366
Tru1I TTAA 8 cut(s) 404, 498, 663, 732, 798, 1007, 1122, 1290
Tru9I TTAA 8 cut(s) 404, 498, 663, 732, 798, 1007, 1122, 1290
TscAI CASTG 4 cut(s) 394, 510, 750, 886
TseFI GTSAC 3 cut(s) 377, 385, 1268
TseI GCWGC 5 cut(s) 368, 876, 1055, 1103, 1187
Tsp45I GTSAC 3 cut(s) 377, 385, 1268
TspDTI ATGAA 8 cut(s) 79, 303, 484, 528, 618, 1164, 1385, 1497
TspGWI ACGGA 1 cut(s) 1129
TspRI CASTG 4 cut(s) 394, 510, 750, 886
Tth111I GACNNNGTC 2 cut(s) 383, 1089
VpaK11BI GGWCC 3 cut(s) 226, 809, 1091
VspI ATTAAT 2 cut(s) 498, 1007
XagI CCTNNNNNAGG 1 cut(s) 1493
XceI RCATGY 2 cut(s) 32, 850
XhoI CTCGAG 1 cut(s) 575
XmiI GTMKAC 1 cut(s) 793
XmnI GAANNNNTTC 1 cut(s) 1134
XspI CTAG 2 cut(s) 459, 1215
Zsp2I ATGCAT 1 cut(s) 922
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.