Rw0G005660

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00223
OG0011117
Physical Location & Seq
Reverse (-)
72235 .. 75786
3552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G005660.1

Sequence Viewer

Length: 1479 bp
ATGGAGGTTAGATCTGAGGGTGTCCATGTCCGCTGCGATAAGCTTCCGTCGCCGAGGATTGCGTCGTCGCCGGTGATTCAGTCGCCCAAGCTTCCGGCGCCGGCGATTCCGTCGCCGGCGATTGCGAGGACGCGCCTGCAGGTGTGGTTCATACGGTTGTGCTCAAGCTTGTTTGTTTGGACTTGTTTGGTTCAGTTGGTGGCGGTTGGGGAGTTTTGGAGTCCGCATTTGTTCTCCAACTTGACCAATCGGATATCCCAAATTACACAGATTCCCTCTCAGGGGGTTCTTTCTCCGCCGCCTCTTCCTCCTGCAAGAAATTATACAAGTAATGGGTTTCTTAGAGTTTCATGCAACGGGGGCTTGAATCAAATGCGTGCTGCGATTTGTGACATGGTGACTGTTGCTCGGCTTTTAAATCTCACTTTGGTTGTTCCAGAGCTTGATAAGACATCTTTCTGGGCCGATCCTAGACATTTCATTGATTCACTGAGAGACGAAGTCCGAATTGTCAGAAGACTGCCAAAAAACTTTAGTAGGAAATATGGGTATAAACCACTCGAGATGCCTCCTGTTAGTTGGTCAAATGAAAAAATACTATCTCGAACAGGTTTCAAGCATAAGGTGTTGCACTTTAACAGAACAGATACACGCTTGGCAAACAATTGGATACCTCTTGATCTTCAGAAACTCAGGTGTCGCGTTAATTTCCAGGCGCTGAAGTTCACTCCAAAGATTGAGTCTTTGGGTTACAAATTGGTTCGTATGCTTCAAGAAAAGGGACCTTTTGTGACTTTGCATCTAAGGTATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCATGGCTGCACAGCTGAAGAAGCTGAGGAGCTCAAACGGTTAAGAGAAGAGGTCTCAAGGGCTGTGTCCTCTGACACCAGAGGAAGTGCATTAATTTTGCAAGCATTGGGTATTAGCAAAGACACACAGATCTATATTGCAGCTGGTGAGATTTATGGCAGGGACAGGAGACTTGCACCGCTAAGAGCTTCGTTTCCCCGAATTGTTAAGAAGGAAACTCTTCTCGCACCAGAGGAATTGCAACAGTTTCAGAATCATTCATCTCAAATGGCTGCTTTGGATTTTATGGTTGCTGTAGCCAGTAATACTTTTGTCCCTACCTATGATGGAAACATGGCAAAGCTTGTTGAAGGTCACCGTAGGTATCTTGGTTTTAAAAAAAGCATCTTGCTAGATCGGAAAAAACTTGTCGAGTTATTAGACATGCATTTAAATGGGACTCTTTCGTGGAATGGGTTTGCACTTGCTGTAAGATCCGTTCATGAGAGAAGAATGGGACAGCCAACTCCGCGAACAGTTATCCCAGAGAAGCCAAAGGAGGAGGACTATTTTTATGCAAATCCTCAGGAGTGCCTCTGCATGGGAACTAATTGTGATGACTTGCTTAGCCGTGGTAACTTAAGTAAACCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

492

Amino Acids

55.85

Weight (kDa)

9.67

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 113 - 404 9.4e-87 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14020 AT2G03280
fragaria_vesca FvH4_4g30810
malus_domestica MD00G1080900.v1.1 MD16G1067800.v1.1
prunus_persica Prupe.1G279000_v2.0.a1
pyrus_communis pycom13g06080 pycom16g05930 pycom16g05940
rosa_chinensis RchiOBHm_Chr4g0439201
rosa_laevigata RLG00000006288
rosa_multiflora Rmu_sc0002236.1_g000012
rosa_roxburghii Rroxscaffold_5G00380190
rosa_rugosa Rorug04G0317000
rosa_samantha Rh4AG367500 Rh4CG393500 Rh4DG373700
rosa_wichuraiana Rw0G005660 Rw0G011650 Rw5G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 3 cut(s) 133, 702, 1354
AciI CCGC 7 cut(s) 31, 203, 224, 296, 299, 1022, 1352
AclWI GGATC 2 cut(s) 461, 1311
AcuI CTGAAG 3 cut(s) 668, 740, 879
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 1 cut(s) 841
AfiI CCNNNNNNNGG 3 cut(s) 281, 282, 1423
AflII CTTAAG 1 cut(s) 1462
AgsI TTSAA 4 cut(s) 367, 616, 773, 1193
AjnI CCWGG 1 cut(s) 711
Alw21I GWGCWC 2 cut(s) 164, 876
Alw26I GTCTC 3 cut(s) 489, 901, 1006
AlwI GGATC 2 cut(s) 461, 1311
AlwNI CAGNNNCTG 1 cut(s) 718
Ama87I CYCGRG 1 cut(s) 560
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 5 cut(s) 33, 380, 849, 983, 1115
ArsI GACNNNNNNTTYG 2 cut(s) 725, 757
AseI ATTAAT 1 cut(s) 935
Asp700I GAANNNNTTC 1 cut(s) 1062
AspA2I CCTAGG 1 cut(s) 1472
AspLEI GCGC 3 cut(s) 100, 135, 718
AspS9I GGNCC 2 cut(s) 462, 782
AsuHPI GGTGA 4 cut(s) 85, 409, 1001, 1190
AvaI CYCGRG 1 cut(s) 560
AvaII GGWCC 1 cut(s) 782
AvrII CCTAGG 1 cut(s) 1472
AxyI CCTNAGG 1 cut(s) 1407
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 876
BbsI GAAGAC 1 cut(s) 523
Bbv12I GWGCWC 2 cut(s) 164, 876
BbvCI CCTCAGC 1 cut(s) 867
BbvI GCAGC 5 cut(s) 20, 367, 836, 995, 1102
BccI CCATC 2 cut(s) 808, 1163
BceAI ACGGC 1 cut(s) 1437
BciT130I CCWGG 1 cut(s) 713
BciVI GTATCC 1 cut(s) 663
BcoDI GTCTC 3 cut(s) 489, 901, 1006
BfaI CTAG 3 cut(s) 471, 1235, 1473
BfmI CTRYAG 2 cut(s) 137, 1137
BfoI RGCGCY 2 cut(s) 101, 719
BfrI CTTAAG 1 cut(s) 1462
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 663
BglII AGATCT 2 cut(s) 11, 972
BisI GCNGC 6 cut(s) 34, 299, 381, 850, 984, 1116
BlnI CCTAGG 1 cut(s) 1472
BlpI GCTNAGC 1 cut(s) 1448
BlsI GCNGC 6 cut(s) 35, 300, 382, 851, 985, 1117
Bme1390I CCNGG 1 cut(s) 713
Bme18I GGWCC 1 cut(s) 782
BmeT110I CYCGRG 1 cut(s) 560
BmgT120I GGNCC 2 cut(s) 462, 782
BmiI GGNNCC 2 cut(s) 99, 783
BmrFI CCNGG 1 cut(s) 713
BmsI GCATC 3 cut(s) 555, 808, 1236
BpiI GAAGAC 1 cut(s) 523
Bpu10I CCTNAGC 1 cut(s) 867
Bpu1102I GCTNAGC 1 cut(s) 1448
BpuEI CTTGAG 2 cut(s) 148, 883
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 901
BsaJI CCNNGG 3 cut(s) 53, 1453, 1472
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 282, 1423
Bse118I RCCGGY 3 cut(s) 70, 100, 115
Bse1I ACTGG 1 cut(s) 1143
Bse21I CCTNAGG 1 cut(s) 1407
BseBI CCWGG 1 cut(s) 713
BseDI CCNNGG 3 cut(s) 53, 1453, 1472
BseLI CCNNNNNNNGG 3 cut(s) 281, 282, 1423
BseMII CTCAG 7 cut(s) 6, 293, 482, 706, 846, 858, 1421
BseNI ACTGG 1 cut(s) 1143
BseRI GAGGAG 2 cut(s) 884, 1397
BseXI GCAGC 5 cut(s) 20, 367, 836, 995, 1102
BsgI GTGCAG 1 cut(s) 835
Bsh1236I CGCG 3 cut(s) 133, 702, 1354
BshFI GGCC 1 cut(s) 464
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 164, 876
BsiHKCI CYCGRG 1 cut(s) 560
BsiSI CCGG 4 cut(s) 71, 95, 101, 116
BslFI GGGAC 5 cut(s) 795, 1019, 1142, 1294, 1353
BslI CCNNNNNNNGG 3 cut(s) 281, 282, 1423
BsmAI GTCTC 3 cut(s) 489, 901, 1006
BsmBI CGTCTC 1 cut(s) 489
BsmFI GGGAC 5 cut(s) 795, 1019, 1142, 1294, 1353
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 1 cut(s) 901
BsoBI CYCGRG 1 cut(s) 560
Bsp1286I GDGCHC 2 cut(s) 164, 876
Bsp143I GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
Bsp1720I GCTNAGC 1 cut(s) 1448
BspACI CCGC 7 cut(s) 31, 203, 224, 296, 299, 1022, 1352
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 7 cut(s) 7, 292, 483, 705, 845, 859, 1420
BspFNI CGCG 3 cut(s) 133, 702, 1354
BspHI TCATGA 1 cut(s) 1324
BspLI GGNNCC 2 cut(s) 99, 783
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 2 cut(s) 461, 1311
BspT107I GGYRCC 1 cut(s) 97
BspTI CTTAAG 1 cut(s) 1462
BspTNI GGTCTC 1 cut(s) 901
BsrFI RCCGGY 3 cut(s) 70, 100, 115
BsrI ACTGG 1 cut(s) 1143
BssAI RCCGGY 3 cut(s) 70, 100, 115
BssECI CCNNGG 3 cut(s) 53, 1453, 1472
BssMI GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 1 cut(s) 1472
Bst2UI CCWGG 1 cut(s) 713
Bst4CI ACNGT 6 cut(s) 156, 403, 882, 1089, 1202, 1360
Bst6I CTCTTC 3 cut(s) 309, 885, 1068
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 1462
BstC8I GCNNGC 6 cut(s) 102, 117, 137, 378, 825, 945
BstDSI CCRYGG 1 cut(s) 1453
BstEII GGTNACC 1 cut(s) 1196
BstFNI CGCG 3 cut(s) 133, 702, 1354
BstH2I RGCGCY 2 cut(s) 101, 719
BstHHI GCGC 3 cut(s) 100, 135, 718
BstKTI GATC 6 cut(s) 14, 469, 682, 975, 1240, 1319
BstMAI GTCTC 3 cut(s) 489, 901, 1006
BstMBI GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
BstMWI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 863, 1351
BstNI CCWGG 1 cut(s) 713
BstNSI RCATGY 2 cut(s) 823, 1270
BstPI GGTNACC 1 cut(s) 1196
BstSCI CCNGG 1 cut(s) 711
BstSFI CTRYAG 2 cut(s) 137, 1137
BstUI CGCG 3 cut(s) 133, 702, 1354
BstV1I GCAGC 5 cut(s) 20, 367, 836, 995, 1102
BstV2I GAAGAC 1 cut(s) 523
BstX2I RGATCY 3 cut(s) 11, 972, 1316
BstYI RGATCY 3 cut(s) 11, 972, 1316
Bsu36I CCTNAGG 1 cut(s) 1407
BsuI GTATCC 1 cut(s) 663
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 1 cut(s) 1453
BtsIMutI CAGTG 1 cut(s) 488
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 6 cut(s) 102, 117, 137, 378, 825, 945
CaiI CAGNNNCTG 1 cut(s) 718
CciI TCATGA 1 cut(s) 1324
CfoI GCGC 3 cut(s) 100, 135, 718
Cfr10I RCCGGY 3 cut(s) 70, 100, 115
Cfr13I GGNCC 2 cut(s) 462, 782
CseI GACGC 2 cut(s) 51, 139
Csp6I GTAC 1 cut(s) 840
CviAII CATG 9 cut(s) 26, 351, 394, 820, 845, 1177, 1267, 1325, 1423
CviQI GTAC 1 cut(s) 840
DinI GGCGCC 1 cut(s) 99
DpnI GATC 6 cut(s) 13, 468, 681, 974, 1239, 1318
DpnII GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
DraI TTTAAA 3 cut(s) 417, 1219, 1275
Eam1104I CTCTTC 3 cut(s) 309, 885, 1068
EarI CTCTTC 3 cut(s) 309, 885, 1068
EciI GGCGGA 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 874
Eco130I CCWWGG 1 cut(s) 1472
Eco24I GRGCYC 1 cut(s) 876
Eco31I GGTCTC 1 cut(s) 901
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 782
Eco53kI GAGCTC 1 cut(s) 874
Eco57I CTGAAG 3 cut(s) 668, 740, 879
Eco81I CCTNAGG 1 cut(s) 1407
Eco88I CYCGRG 1 cut(s) 560
Eco91I GGTNACC 1 cut(s) 1196
EcoICRI GAGCTC 1 cut(s) 874
EcoO109I RGGNCCY 1 cut(s) 782
EcoO65I GGTNACC 1 cut(s) 1196
EcoRII CCWGG 1 cut(s) 711
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 1 cut(s) 1472
EcoT22I ATGCAT 1 cut(s) 1272
EcoT38I GRGCYC 1 cut(s) 876
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 1472
Esp3I CGTCTC 1 cut(s) 489
FaeI CATG 9 cut(s) 29, 354, 397, 823, 848, 1180, 1270, 1328, 1426
FaqI GGGAC 5 cut(s) 795, 1019, 1142, 1294, 1353
FatI CATG 9 cut(s) 25, 350, 393, 819, 844, 1176, 1266, 1324, 1422
Fnu4HI GCNGC 6 cut(s) 34, 299, 381, 850, 984, 1116
FriOI GRGCYC 1 cut(s) 876
Fsp4HI GCNGC 6 cut(s) 34, 299, 381, 850, 984, 1116
FspBI CTAG 3 cut(s) 471, 1235, 1473
GlaI GCGC 3 cut(s) 99, 134, 717
GluI GCNGC 6 cut(s) 34, 299, 381, 850, 984, 1116
HaeII RGCGCY 2 cut(s) 101, 719
HaeIII GGCC 1 cut(s) 464
HapII CCGG 4 cut(s) 71, 95, 101, 116
HgaI GACGC 2 cut(s) 51, 139
HhaI GCGC 3 cut(s) 100, 135, 718
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 9 cut(s) 29, 354, 397, 823, 848, 1180, 1270, 1328, 1426
Hin6I GCGC 3 cut(s) 98, 133, 716
HinP1I GCGC 3 cut(s) 98, 133, 716
HindIII AAGCTT 4 cut(s) 41, 89, 166, 1184
HinfI GANTC 9 cut(s) 76, 106, 220, 271, 367, 485, 740, 1096, 1282
HpaII CCGG 4 cut(s) 71, 95, 101, 116
HphI GGTGA 4 cut(s) 85, 409, 1001, 1190
Hpy166II GTNNAC 2 cut(s) 726, 1469
Hpy188I TCNGA 8 cut(s) 16, 252, 506, 515, 687, 916, 1095, 1242
Hpy188III TCNNGA 7 cut(s) 437, 562, 603, 677, 773, 1325, 1409
Hpy8I GTNNAC 2 cut(s) 726, 1469
Hpy99I CGWCG 4 cut(s) 52, 67, 70, 115
HpyAV CCTTC 2 cut(s) 1048, 1187
HpyCH4III ACNGT 6 cut(s) 156, 403, 882, 1089, 1202, 1360
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 863, 1351
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 9 cut(s) 29, 354, 397, 823, 848, 1180, 1270, 1328, 1426
HspAI GCGC 3 cut(s) 98, 133, 716
KasI GGCGCC 1 cut(s) 97
KroI GCCGGC 2 cut(s) 100, 115
KroNI GCCGGC 2 cut(s) 102, 117
Kzo9I GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
LmnI GCTCC 1 cut(s) 871
Lsp1109I GCAGC 5 cut(s) 20, 367, 836, 995, 1102
LweI GCATC 3 cut(s) 555, 808, 1236
MaeI CTAG 3 cut(s) 471, 1235, 1473
MaeIII GTNAC 6 cut(s) 389, 397, 749, 790, 1196, 1457
MalI GATC 6 cut(s) 13, 468, 681, 974, 1239, 1318
MboI GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
MboII GAAGA 7 cut(s) 296, 528, 674, 872, 902, 1055, 1344
MfeI CAATTG 1 cut(s) 664
MflI RGATCY 3 cut(s) 11, 972, 1316
MhlI GDGCHC 2 cut(s) 164, 876
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 3 cut(s) 229, 749, 1276
MmeI TCCRAC 1 cut(s) 261
Mph1103I ATGCAT 1 cut(s) 1272
MreI CGCCGGCG 2 cut(s) 100, 115
MroNI GCCGGC 2 cut(s) 100, 115
MroXI GAANNNNTTC 1 cut(s) 1062
MseI TTAA 9 cut(s) 416, 636, 705, 884, 935, 1050, 1218, 1274, 1463
MslI CAYNNNNRTG 1 cut(s) 1275
MspA1I CMGCKG 3 cut(s) 33, 857, 986
MspCI CTTAAG 1 cut(s) 1462
MspI CCGG 4 cut(s) 71, 95, 101, 116
MspR9I CCNGG 1 cut(s) 713
MunI CAATTG 1 cut(s) 664
MvaI CCWGG 1 cut(s) 713
MvnI CGCG 3 cut(s) 133, 702, 1354
MwoI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 863, 1351
NaeI GCCGGC 2 cut(s) 102, 117
NarI GGCGCC 1 cut(s) 98
NdeII GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
NgoMIV GCCGGC 2 cut(s) 100, 115
NlaIII CATG 9 cut(s) 29, 354, 397, 823, 848, 1180, 1270, 1328, 1426
NlaIV GGNNCC 2 cut(s) 99, 783
NmeAIII GCCGAG 2 cut(s) 78, 388
NmuCI GTSAC 4 cut(s) 389, 397, 790, 1196
NsiI ATGCAT 1 cut(s) 1272
NspI RCATGY 2 cut(s) 823, 1270
PaeR7I CTCGAG 1 cut(s) 560
PagI TCATGA 1 cut(s) 1324
PaqCI CACCTGC 1 cut(s) 130
PdiI GCCGGC 2 cut(s) 102, 117
PdmI GAANNNNTTC 1 cut(s) 1062
PfeI GAWTC 6 cut(s) 76, 106, 271, 367, 485, 1096
PflFI GACNNNGTC 1 cut(s) 500
PkrI GCNGC 6 cut(s) 35, 300, 382, 851, 985, 1117
PleI GAGTC 3 cut(s) 228, 748, 1276
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 3 cut(s) 228, 748, 1276
PpuMI RGGWCCY 1 cut(s) 782
PshBI ATTAAT 1 cut(s) 935
Psp124BI GAGCTC 1 cut(s) 876
Psp5II RGGWCCY 1 cut(s) 782
Psp6I CCWGG 1 cut(s) 711
PspEI GGTNACC 1 cut(s) 1196
PspGI CCWGG 1 cut(s) 711
PspN4I GGNNCC 2 cut(s) 99, 783
PspPI GGNCC 2 cut(s) 462, 782
PspPPI RGGWCCY 1 cut(s) 782
PsrI GAACNNNNNNTAC 2 cut(s) 1305, 1337
PstI CTGCAG 1 cut(s) 141
PstNI CAGNNNCTG 1 cut(s) 718
PsuI RGATCY 3 cut(s) 11, 972, 1316
PsyI GACNNNGTC 1 cut(s) 500
PvuII CAGCTG 2 cut(s) 857, 986
RsaI GTAC 1 cut(s) 841
RsaNI GTAC 1 cut(s) 840
RseI CAYNNNNRTG 1 cut(s) 1275
SacI GAGCTC 1 cut(s) 876
SaqAI TTAA 9 cut(s) 416, 636, 705, 884, 935, 1050, 1218, 1274, 1463
SatI GCNGC 6 cut(s) 34, 299, 381, 850, 984, 1116
Sau3AI GATC 6 cut(s) 11, 466, 679, 972, 1237, 1316
Sau96I GGNCC 2 cut(s) 462, 782
SbfI CCTGCAGG 1 cut(s) 141
SchI GAGTC 3 cut(s) 229, 749, 1276
ScrFI CCNGG 1 cut(s) 713
SdaI CCTGCAGG 1 cut(s) 141
SduI GDGCHC 2 cut(s) 164, 876
SfaNI GCATC 3 cut(s) 555, 808, 1236
SfcI CTRYAG 2 cut(s) 137, 1137
SfoI GGCGCC 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 560
SgrAI CRCCGGYG 3 cut(s) 70, 100, 115
SinI GGWCC 1 cut(s) 782
SlaI CTCGAG 1 cut(s) 560
SmiI ATTTAAAT 1 cut(s) 1275
SmiMI CAYNNNNRTG 1 cut(s) 1275
SmlI CTYRAG 4 cut(s) 163, 560, 898, 1462
SmoI CTYRAG 4 cut(s) 163, 560, 898, 1462
Sse8387I CCTGCAGG 1 cut(s) 141
SsiI CCGC 7 cut(s) 31, 203, 224, 296, 299, 1022, 1352
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 3 cut(s) 471, 1235, 1473
SstI GAGCTC 1 cut(s) 876
StyD4I CCNGG 1 cut(s) 711
StyI CCWWGG 1 cut(s) 1472
SwaI ATTTAAAT 1 cut(s) 1275
TaaI ACNGT 6 cut(s) 156, 403, 882, 1089, 1202, 1360
TaqI TCGA 3 cut(s) 561, 604, 1254
TatI WGTACW 1 cut(s) 839
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 6 cut(s) 76, 106, 271, 367, 485, 1096
Tru1I TTAA 9 cut(s) 416, 636, 705, 884, 935, 1050, 1218, 1274, 1463
Tru9I TTAA 9 cut(s) 416, 636, 705, 884, 935, 1050, 1218, 1274, 1463
TscAI CASTG 1 cut(s) 495
TseFI GTSAC 4 cut(s) 389, 397, 790, 1196
TseI GCWGC 5 cut(s) 33, 380, 849, 983, 1115
Tsp45I GTSAC 4 cut(s) 389, 397, 790, 1196
TspDTI ATGAA 6 cut(s) 139, 339, 469, 603, 1092, 1313
TspGWI ACGGA 3 cut(s) 36, 99, 1309
TspRI CASTG 1 cut(s) 495
Tth111I GACNNNGTC 1 cut(s) 500
Vha464I CTTAAG 1 cut(s) 1462
VpaK11BI GGWCC 1 cut(s) 782
VspI ATTAAT 1 cut(s) 935
XceI RCATGY 2 cut(s) 823, 1270
XhoI CTCGAG 1 cut(s) 560
XmaJI CCTAGG 1 cut(s) 1472
XmnI GAANNNNTTC 1 cut(s) 1062
XspI CTAG 3 cut(s) 471, 1235, 1473
Zsp2I ATGCAT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.