Rw5G028530

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011117
Physical Location & Seq
Forward (+)
43255458 .. 43259016
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028530.1

Sequence Viewer

Length: 1563 bp
ATGGAGGTTAGATCTGAGGGTGTCCATGTCCGCTGCGATAAGCTTCCGTCGCCGAGGATTGCGTCGTCGCCGGTGATTCAGTCGCCCAAGCTTCCGGCGCCGGCGATTCCGTCGCCGGCGATTGCGAGGACGCGCCTGCAGGTGTGGTTCATACGGTTGTGCTCAAGCTTGTTTGTTTGGACTTGTTTGGTTCAGTTGGTGGCGGTTGGGGAGTTTTGGAGTCCGCATTTGTTCTCCAACTTAACCAATCGGATATCCCAAATTACACAGATTCCCTCTCAGGGGGTTCTTTCTCCGCCGCCTCTTCCTCCTGCAAGAAATTATACAAGTAATGGGTTTCTTAGAGTTTCATGCAACGGGGGCTTGAATCAAATGCGTGCTGCGATTTGTGACATGGTGACTGTTGCTCGGCTTTTAAATCTCACTTTGGTTGTTCCAGAGCTTGATAAGACATCTTTCTGGGCCGATCCTAGTAATTTTGAGGATATATTTGATTTAAGACATTTCATTGATTCACTGAGAGACGAAGTCCGAATTGTCAGAAGACTGCCAAAAAACTTTAGTAGGAAATATGGGTATAAACCACTCGAGATGCCTCCTGTTAGTTGGTCAAATGAAAAATACTATCTCGAACAGATTCTGCCACTTGTTAGCAAGCATAAGGTGTTGCACTTTAACAGAACAGATACACGCTTGGCAAACAATTGGATACCTCTTGATCTTCAGAAACTCAGGTGTCGCGTTAATTTCCAGGCGCTGAAGTTCACTCCAAAGATTGAGTCTTTGGGTTACAAATTGGTTCGTATGCTTCAAGAAAAGGGACCTTTTGTGACTTTGCATCTAAGGTATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCATGGCTGCACAGCTGAAGAAGCTGAGGAGCTCAAACGGTTAAGGTATGCATATCCTTGGTGGAGAGAGAAAGAGATAGTGTCTGAAGAGAAGAGGTCTCAAGGGCTGTGTCCTCTGACACCAGAGGAAAGTGCATTAATTTTGCAAGCATTGGGTATTAGCAAAGACACACAGATCTATATTGCAGCTGGTGAGATTTATGGCAGGGACAGGAGACTTGCACCGCTAAGAGCTTCGTTTCCCCGAATTGTTAAGAAGGAAACTCTTCTCGCACCAGAGGAATTGCAACAGTTTCAGAATCATTCATCTCAAATGGCTGCTTTGGATTTTATGGTTGCGGTAGCCAGTAATACTTTTGTCCCTACCTATGATGGAAACATGGCAAAGCTTGTTGAAGGTCACCGTAGGTATCTTGGTTTTAAAAAAAGCATCTTGCTAGATCGGAAAAAACTTGTCGAGTTATTAGACATGCATTTAAATGGGACTCTTTCGTGGAATGGGTTTGCACTTGCTGTAAGATCCGTTCATGAGAGAAGAATGGGACAGCCAACTCCGCGAACAGTTATCCCAGAGAAGCCAAAGGAGGAGGACTATTTTTATGCAAATCCTCAGGAGTGCCTCTGCATGGGAACTAATTGTGATGACTTGCTTAGCCGTGGTAACTTAAGTAAACCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

520

Amino Acids

59.51

Weight (kDa)

9.29

Isoelectric Point (pI)

54.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 113 - 432 6.9e-108 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14020 AT2G03280
fragaria_vesca FvH4_4g30810
malus_domestica MD00G1080900.v1.1 MD16G1067800.v1.1
prunus_persica Prupe.1G279000_v2.0.a1
pyrus_communis pycom13g06080 pycom16g05930 pycom16g05940
rosa_chinensis RchiOBHm_Chr4g0439201
rosa_laevigata RLG00000006288
rosa_multiflora Rmu_sc0002236.1_g000012
rosa_roxburghii Rroxscaffold_5G00380190
rosa_rugosa Rorug04G0317000
rosa_samantha Rh4AG367500 Rh4CG393500 Rh4DG373700
rosa_wichuraiana Rw0G005660 Rw0G011650 Rw5G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 130
Acc36I ACCTGC 1 cut(s) 130
AccB1I GGYRCC 1 cut(s) 97
AccII CGCG 3 cut(s) 133, 741, 1438
AciI CCGC 8 cut(s) 31, 203, 224, 296, 299, 1106, 1220, 1436
AclWI GGATC 2 cut(s) 461, 1395
AcuI CTGAAG 4 cut(s) 707, 779, 918, 987
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 1 cut(s) 880
AfiI CCNNNNNNNGG 3 cut(s) 281, 282, 1507
AflII CTTAAG 1 cut(s) 1546
AgsI TTSAA 3 cut(s) 367, 812, 1277
AjnI CCWGG 1 cut(s) 750
Alw21I GWGCWC 2 cut(s) 164, 915
Alw26I GTCTC 3 cut(s) 516, 984, 1090
AlwI GGATC 2 cut(s) 461, 1395
AlwNI CAGNNNCTG 2 cut(s) 640, 757
Ama87I CYCGRG 1 cut(s) 587
AoxI GGCC 1 cut(s) 462
ApeKI GCWGC 5 cut(s) 33, 380, 888, 1067, 1199
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
AseI ATTAAT 1 cut(s) 1019
Asp700I GAANNNNTTC 2 cut(s) 636, 1146
AspA2I CCTAGG 1 cut(s) 1556
AspLEI GCGC 3 cut(s) 100, 135, 757
AspS9I GGNCC 2 cut(s) 462, 821
AsuHPI GGTGA 4 cut(s) 85, 409, 1085, 1274
AvaI CYCGRG 1 cut(s) 587
AvaII GGWCC 1 cut(s) 821
AvrII CCTAGG 1 cut(s) 1556
AxyI CCTNAGG 1 cut(s) 1491
BanI GGYRCC 1 cut(s) 97
BanII GRGCYC 1 cut(s) 915
BbsI GAAGAC 1 cut(s) 550
Bbv12I GWGCWC 2 cut(s) 164, 915
BbvCI CCTCAGC 1 cut(s) 906
BbvI GCAGC 5 cut(s) 20, 367, 875, 1079, 1186
BccI CCATC 2 cut(s) 847, 1247
BceAI ACGGC 1 cut(s) 1521
BciT130I CCWGG 1 cut(s) 752
BciVI GTATCC 1 cut(s) 702
BcoDI GTCTC 3 cut(s) 516, 984, 1090
BfaI CTAG 3 cut(s) 471, 1319, 1557
BfmI CTRYAG 1 cut(s) 137
BfoI RGCGCY 2 cut(s) 101, 758
BfrI CTTAAG 1 cut(s) 1546
BfuAI ACCTGC 1 cut(s) 130
BfuI GTATCC 1 cut(s) 702
BglII AGATCT 2 cut(s) 11, 1056
BisI GCNGC 6 cut(s) 34, 299, 381, 889, 1068, 1200
BlnI CCTAGG 1 cut(s) 1556
BlpI GCTNAGC 1 cut(s) 1532
BlsI GCNGC 6 cut(s) 35, 300, 382, 890, 1069, 1201
Bme1390I CCNGG 1 cut(s) 752
Bme18I GGWCC 1 cut(s) 821
BmeT110I CYCGRG 1 cut(s) 587
BmgT120I GGNCC 2 cut(s) 462, 821
BmiI GGNNCC 2 cut(s) 99, 822
BmrFI CCNGG 1 cut(s) 752
BmsI GCATC 3 cut(s) 582, 847, 1320
BpiI GAAGAC 1 cut(s) 550
Bpu10I CCTNAGC 1 cut(s) 906
Bpu1102I GCTNAGC 1 cut(s) 1532
BpuEI CTTGAG 2 cut(s) 148, 966
BsaHI GRCGYC 1 cut(s) 98
BsaI GGTCTC 1 cut(s) 984
BsaJI CCNNGG 4 cut(s) 53, 938, 1537, 1556
Bsc4I CCNNNNNNNGG 3 cut(s) 281, 282, 1507
Bse118I RCCGGY 3 cut(s) 70, 100, 115
Bse1I ACTGG 1 cut(s) 1227
Bse21I CCTNAGG 1 cut(s) 1491
BseBI CCWGG 1 cut(s) 752
BseDI CCNNGG 4 cut(s) 53, 938, 1537, 1556
BseLI CCNNNNNNNGG 3 cut(s) 281, 282, 1507
BseMII CTCAG 7 cut(s) 6, 293, 509, 745, 885, 897, 1505
BseNI ACTGG 1 cut(s) 1227
BseRI GAGGAG 2 cut(s) 923, 1481
BseXI GCAGC 5 cut(s) 20, 367, 875, 1079, 1186
BsgI GTGCAG 1 cut(s) 874
Bsh1236I CGCG 3 cut(s) 133, 741, 1438
BshFI GGCC 1 cut(s) 464
BshNI GGYRCC 1 cut(s) 97
BsiHKAI GWGCWC 2 cut(s) 164, 915
BsiHKCI CYCGRG 1 cut(s) 587
BsiSI CCGG 4 cut(s) 71, 95, 101, 116
BslFI GGGAC 5 cut(s) 834, 1103, 1226, 1378, 1437
BslI CCNNNNNNNGG 3 cut(s) 281, 282, 1507
BsmAI GTCTC 3 cut(s) 516, 984, 1090
BsmBI CGTCTC 1 cut(s) 516
BsmFI GGGAC 5 cut(s) 834, 1103, 1226, 1378, 1437
BsnI GGCC 1 cut(s) 464
Bso31I GGTCTC 1 cut(s) 984
BsoBI CYCGRG 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 164, 915
Bsp143I GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
Bsp1720I GCTNAGC 1 cut(s) 1532
BspACI CCGC 8 cut(s) 31, 203, 224, 296, 299, 1106, 1220, 1436
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 7 cut(s) 7, 292, 510, 744, 884, 898, 1504
BspFNI CGCG 3 cut(s) 133, 741, 1438
BspHI TCATGA 1 cut(s) 1408
BspLI GGNNCC 2 cut(s) 99, 822
BspMAI CTGCAG 1 cut(s) 141
BspMI ACCTGC 1 cut(s) 130
BspPI GGATC 2 cut(s) 461, 1395
BspT107I GGYRCC 1 cut(s) 97
BspTI CTTAAG 1 cut(s) 1546
BspTNI GGTCTC 1 cut(s) 984
BsrFI RCCGGY 3 cut(s) 70, 100, 115
BsrI ACTGG 1 cut(s) 1227
BssAI RCCGGY 3 cut(s) 70, 100, 115
BssECI CCNNGG 4 cut(s) 53, 938, 1537, 1556
BssMI GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 938, 1556
Bst2UI CCWGG 1 cut(s) 752
Bst4CI ACNGT 6 cut(s) 156, 403, 921, 1173, 1286, 1444
Bst6I CTCTTC 4 cut(s) 309, 963, 968, 1152
BstACI GRCGYC 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 1546
BstC8I GCNNGC 7 cut(s) 102, 117, 137, 378, 656, 864, 1029
BstDSI CCRYGG 1 cut(s) 1537
BstEII GGTNACC 1 cut(s) 1280
BstFNI CGCG 3 cut(s) 133, 741, 1438
BstH2I RGCGCY 2 cut(s) 101, 758
BstHHI GCGC 3 cut(s) 100, 135, 757
BstKTI GATC 6 cut(s) 14, 469, 721, 1059, 1324, 1403
BstMAI GTCTC 3 cut(s) 516, 984, 1090
BstMBI GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
BstMWI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 902, 1435
BstNI CCWGG 1 cut(s) 752
BstNSI RCATGY 2 cut(s) 862, 1354
BstPI GGTNACC 1 cut(s) 1280
BstSCI CCNGG 1 cut(s) 750
BstSFI CTRYAG 1 cut(s) 137
BstUI CGCG 3 cut(s) 133, 741, 1438
BstV1I GCAGC 5 cut(s) 20, 367, 875, 1079, 1186
BstV2I GAAGAC 1 cut(s) 550
BstX2I RGATCY 3 cut(s) 11, 1056, 1400
BstYI RGATCY 3 cut(s) 11, 1056, 1400
Bsu36I CCTNAGG 1 cut(s) 1491
BsuI GTATCC 1 cut(s) 702
BsuRI GGCC 1 cut(s) 464
BtgI CCRYGG 1 cut(s) 1537
BtsIMutI CAGTG 1 cut(s) 515
BveI ACCTGC 1 cut(s) 130
Cac8I GCNNGC 7 cut(s) 102, 117, 137, 378, 656, 864, 1029
CaiI CAGNNNCTG 2 cut(s) 640, 757
CciI TCATGA 1 cut(s) 1408
CfoI GCGC 3 cut(s) 100, 135, 757
Cfr10I RCCGGY 3 cut(s) 70, 100, 115
Cfr13I GGNCC 2 cut(s) 462, 821
CseI GACGC 2 cut(s) 51, 139
Csp6I GTAC 1 cut(s) 879
CviAII CATG 9 cut(s) 26, 351, 394, 859, 884, 1261, 1351, 1409, 1507
CviQI GTAC 1 cut(s) 879
DinI GGCGCC 1 cut(s) 99
DpnI GATC 6 cut(s) 13, 468, 720, 1058, 1323, 1402
DpnII GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
DraI TTTAAA 3 cut(s) 417, 1303, 1359
Eam1104I CTCTTC 4 cut(s) 309, 963, 968, 1152
EarI CTCTTC 4 cut(s) 309, 963, 968, 1152
EciI GGCGGA 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 913
Eco130I CCWWGG 2 cut(s) 938, 1556
Eco24I GRGCYC 1 cut(s) 915
Eco31I GGTCTC 1 cut(s) 984
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 821
Eco53kI GAGCTC 1 cut(s) 913
Eco57I CTGAAG 4 cut(s) 707, 779, 918, 987
Eco81I CCTNAGG 1 cut(s) 1491
Eco88I CYCGRG 1 cut(s) 587
Eco91I GGTNACC 1 cut(s) 1280
EcoICRI GAGCTC 1 cut(s) 913
EcoO109I RGGNCCY 1 cut(s) 821
EcoO65I GGTNACC 1 cut(s) 1280
EcoRII CCWGG 1 cut(s) 750
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 938, 1556
EcoT22I ATGCAT 2 cut(s) 934, 1356
EcoT38I GRGCYC 1 cut(s) 915
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 938, 1556
Esp3I CGTCTC 1 cut(s) 516
FaeI CATG 9 cut(s) 29, 354, 397, 862, 887, 1264, 1354, 1412, 1510
FaqI GGGAC 5 cut(s) 834, 1103, 1226, 1378, 1437
FatI CATG 9 cut(s) 25, 350, 393, 858, 883, 1260, 1350, 1408, 1506
Fnu4HI GCNGC 6 cut(s) 34, 299, 381, 889, 1068, 1200
FriOI GRGCYC 1 cut(s) 915
Fsp4HI GCNGC 6 cut(s) 34, 299, 381, 889, 1068, 1200
FspBI CTAG 3 cut(s) 471, 1319, 1557
GlaI GCGC 3 cut(s) 99, 134, 756
GluI GCNGC 6 cut(s) 34, 299, 381, 889, 1068, 1200
HaeII RGCGCY 2 cut(s) 101, 758
HaeIII GGCC 1 cut(s) 464
HapII CCGG 4 cut(s) 71, 95, 101, 116
HgaI GACGC 2 cut(s) 51, 139
HhaI GCGC 3 cut(s) 100, 135, 757
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 9 cut(s) 29, 354, 397, 862, 887, 1264, 1354, 1412, 1510
Hin6I GCGC 3 cut(s) 98, 133, 755
HinP1I GCGC 3 cut(s) 98, 133, 755
HindIII AAGCTT 4 cut(s) 41, 89, 166, 1268
HpaII CCGG 4 cut(s) 71, 95, 101, 116
HphI GGTGA 4 cut(s) 85, 409, 1085, 1274
Hpy166II GTNNAC 2 cut(s) 765, 1553
Hpy188I TCNGA 9 cut(s) 16, 252, 533, 542, 726, 967, 999, 1179, 1326
Hpy188III TCNNGA 7 cut(s) 437, 589, 629, 716, 812, 1409, 1493
Hpy8I GTNNAC 2 cut(s) 765, 1553
Hpy99I CGWCG 4 cut(s) 52, 67, 70, 115
HpyAV CCTTC 2 cut(s) 1132, 1271
HpyCH4III ACNGT 6 cut(s) 156, 403, 921, 1173, 1286, 1444
HpyF10VI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 902, 1435
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 9 cut(s) 29, 354, 397, 862, 887, 1264, 1354, 1412, 1510
HspAI GCGC 3 cut(s) 98, 133, 755
KasI GGCGCC 1 cut(s) 97
KroI GCCGGC 2 cut(s) 100, 115
KroNI GCCGGC 2 cut(s) 102, 117
Kzo9I GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
LmnI GCTCC 1 cut(s) 910
Lsp1109I GCAGC 5 cut(s) 20, 367, 875, 1079, 1186
LweI GCATC 3 cut(s) 582, 847, 1320
MaeI CTAG 3 cut(s) 471, 1319, 1557
MaeIII GTNAC 6 cut(s) 389, 397, 788, 829, 1280, 1541
MalI GATC 6 cut(s) 13, 468, 720, 1058, 1323, 1402
MboI GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
MboII GAAGA 8 cut(s) 296, 555, 713, 911, 980, 985, 1139, 1428
MfeI CAATTG 1 cut(s) 703
MflI RGATCY 3 cut(s) 11, 1056, 1400
MhlI GDGCHC 2 cut(s) 164, 915
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 3 cut(s) 229, 788, 1360
MmeI TCCRAC 1 cut(s) 261
Mph1103I ATGCAT 2 cut(s) 934, 1356
MreI CGCCGGCG 2 cut(s) 100, 115
MroNI GCCGGC 2 cut(s) 100, 115
MroXI GAANNNNTTC 2 cut(s) 636, 1146
MslI CAYNNNNRTG 1 cut(s) 1359
MspA1I CMGCKG 3 cut(s) 33, 896, 1070
MspCI CTTAAG 1 cut(s) 1546
MspI CCGG 4 cut(s) 71, 95, 101, 116
MspR9I CCNGG 1 cut(s) 752
MunI CAATTG 1 cut(s) 703
MvaI CCWGG 1 cut(s) 752
MvnI CGCG 3 cut(s) 133, 741, 1438
MwoI GCNNNNNNNGC 5 cut(s) 49, 97, 360, 902, 1435
NaeI GCCGGC 2 cut(s) 102, 117
NarI GGCGCC 1 cut(s) 98
NdeII GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
NgoMIV GCCGGC 2 cut(s) 100, 115
NlaIII CATG 9 cut(s) 29, 354, 397, 862, 887, 1264, 1354, 1412, 1510
NlaIV GGNNCC 2 cut(s) 99, 822
NmeAIII GCCGAG 2 cut(s) 78, 388
NmuCI GTSAC 4 cut(s) 389, 397, 829, 1280
NsiI ATGCAT 2 cut(s) 934, 1356
NspI RCATGY 2 cut(s) 862, 1354
PaeR7I CTCGAG 1 cut(s) 587
PagI TCATGA 1 cut(s) 1408
PaqCI CACCTGC 1 cut(s) 130
PdiI GCCGGC 2 cut(s) 102, 117
PdmI GAANNNNTTC 2 cut(s) 636, 1146
PfeI GAWTC 7 cut(s) 76, 106, 271, 367, 512, 637, 1180
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 6 cut(s) 35, 300, 382, 890, 1069, 1201
PleI GAGTC 3 cut(s) 228, 787, 1360
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 3 cut(s) 228, 787, 1360
PpuMI RGGWCCY 1 cut(s) 821
PshBI ATTAAT 1 cut(s) 1019
Psp124BI GAGCTC 1 cut(s) 915
Psp5II RGGWCCY 1 cut(s) 821
Psp6I CCWGG 1 cut(s) 750
PspEI GGTNACC 1 cut(s) 1280
PspGI CCWGG 1 cut(s) 750
PspN4I GGNNCC 2 cut(s) 99, 822
PspPI GGNCC 2 cut(s) 462, 821
PspPPI RGGWCCY 1 cut(s) 821
PsrI GAACNNNNNNTAC 2 cut(s) 1389, 1421
PstI CTGCAG 1 cut(s) 141
PstNI CAGNNNCTG 2 cut(s) 640, 757
PsuI RGATCY 3 cut(s) 11, 1056, 1400
PsyI GACNNNGTC 1 cut(s) 527
PvuII CAGCTG 2 cut(s) 896, 1070
RsaI GTAC 1 cut(s) 880
RsaNI GTAC 1 cut(s) 879
RseI CAYNNNNRTG 1 cut(s) 1359
SacI GAGCTC 1 cut(s) 915
SatI GCNGC 6 cut(s) 34, 299, 381, 889, 1068, 1200
Sau3AI GATC 6 cut(s) 11, 466, 718, 1056, 1321, 1400
Sau96I GGNCC 2 cut(s) 462, 821
SbfI CCTGCAGG 1 cut(s) 141
SchI GAGTC 3 cut(s) 229, 788, 1360
ScrFI CCNGG 1 cut(s) 752
SdaI CCTGCAGG 1 cut(s) 141
SduI GDGCHC 2 cut(s) 164, 915
SfaNI GCATC 3 cut(s) 582, 847, 1320
SfcI CTRYAG 1 cut(s) 137
SfoI GGCGCC 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 587
SgrAI CRCCGGYG 3 cut(s) 70, 100, 115
SinI GGWCC 1 cut(s) 821
SlaI CTCGAG 1 cut(s) 587
SmiI ATTTAAAT 1 cut(s) 1359
SmiMI CAYNNNNRTG 1 cut(s) 1359
SmlI CTYRAG 4 cut(s) 163, 587, 981, 1546
SmoI CTYRAG 4 cut(s) 163, 587, 981, 1546
Sse8387I CCTGCAGG 1 cut(s) 141
SsiI CCGC 8 cut(s) 31, 203, 224, 296, 299, 1106, 1220, 1436
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 3 cut(s) 471, 1319, 1557
SstI GAGCTC 1 cut(s) 915
StyD4I CCNGG 1 cut(s) 750
StyI CCWWGG 2 cut(s) 938, 1556
SwaI ATTTAAAT 1 cut(s) 1359
TaaI ACNGT 6 cut(s) 156, 403, 921, 1173, 1286, 1444
TaqI TCGA 3 cut(s) 588, 630, 1338
TatI WGTACW 1 cut(s) 878
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 7 cut(s) 76, 106, 271, 367, 512, 637, 1180
TscAI CASTG 1 cut(s) 522
TseFI GTSAC 4 cut(s) 389, 397, 829, 1280
TseI GCWGC 5 cut(s) 33, 380, 888, 1067, 1199
Tsp45I GTSAC 4 cut(s) 389, 397, 829, 1280
TspDTI ATGAA 6 cut(s) 139, 339, 496, 630, 1176, 1397
TspGWI ACGGA 3 cut(s) 36, 99, 1393
TspRI CASTG 1 cut(s) 522
Tth111I GACNNNGTC 1 cut(s) 527
Vha464I CTTAAG 1 cut(s) 1546
VpaK11BI GGWCC 1 cut(s) 821
VspI ATTAAT 1 cut(s) 1019
XceI RCATGY 2 cut(s) 862, 1354
XhoI CTCGAG 1 cut(s) 587
XmaJI CCTAGG 1 cut(s) 1556
XmnI GAANNNNTTC 2 cut(s) 636, 1146
XspI CTAG 3 cut(s) 471, 1319, 1557
Zsp2I ATGCAT 2 cut(s) 934, 1356
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.