Rh4DG373700

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0011117
Physical Location & Seq
Forward (+)
59150777 .. 59155660
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG373700.1

Sequence Viewer

Length: 1191 bp
ATGCGTGCTGCGATTTGTGACATGGTGACTGTTGCTCGGCTTTTAAATCTCACTTTGGTTGTTCCAGAGCTTGATAAGACATCTTTCTGGGCCGATCCTAGTAATTTTGAGGATATATTTGATTTAAGACATTTCATTGATTCACTGAGAGACGAAGTCCGAATTGTCAGAAGACTGCCAAAAAACTTTAGTAGGAAATATGGGTATAAACCACTCGAGATGCCTCCTGTTAGTTGGTCAAATGAAAAATACTATCTCGAACAGATTCTGCCACTTGTTAGCAAGCATAAGGTGTTGCACTTTAACAGAACAGATACACGCTTGGCAAACAATTGGATACCTCTTGATCTTCAGAAACTCAGGTGTCGCGTCAATTTCCAGGCACTGAAGTTCACTCCAAAGATTGAGTCTTTGGGTTACAAATTGGTTCGTATGCTTCAAGAAAAGGGACCTTTCGTGACTTTGCATCTAAGGTATGAGATGGACATGCTGGCTTTCTCAGGTTGTACTCATGGCTGCACAGCTGAAGAAGCCGAGGAGCTCAAACGGTTAAGGTATGCATATCCTTGGTGGAGAGAGAAAGAGATAGTGTCTGAAGAGAAGAGGTCTCAAGGGCTGTGTCCTCTGACACCAGAGGAAAGTGCATTAATTTTGCAAGCATTGGGTATTAGCAAAGACACACAGATCTATATTGCAGCTGGTGAGATTTATGGCAGGGACAGGAGACTTGCACCGCTAAGAGCTTCGTTTCCCCGAATTGTTAAGAAGGAAACTCTTCTCGCACCAGAGGAATTGCAACAATTTCAGAATCATTCATCTCAAATGGCTGCTTTGGATTTTATGGTTGCGGTAGCCAGTAATACTTTTGTCCCTACCTATGATGGAAACATGGCAAAGCTTGTTGAAGGTCACCGTAGGTATCTTGGTTTTAAAAAAAGCATCTTGCTAGATCGGAAAAAACTTGTCGAGTTATTAGACATGCATTTAAATGGGACTCTTTCGTGGAATGGGTTTGCACTTGCTGTAAGATCCGTTCATGAGAGAAGAATGGGACAGCCAACTCCGCGAACAGTTATCCCAGAGAAGCCAAAGGAGGAGGACTATTTTTATGCAAATCCTCAGGAGTGCCTCTGCATGGGAACGAATTGTGATGACTTGCTTAGCCGTGGTAACTTAAGTAAACCTAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

396

Amino Acids

45.92

Weight (kDa)

9.03

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 1 - 308 4.4e-101 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14020 AT2G03280
fragaria_vesca FvH4_4g30810
malus_domestica MD00G1080900.v1.1 MD16G1067800.v1.1
prunus_persica Prupe.1G279000_v2.0.a1
pyrus_communis pycom13g06080 pycom16g05930 pycom16g05940
rosa_chinensis RchiOBHm_Chr4g0439201
rosa_laevigata RLG00000006288
rosa_multiflora Rmu_sc0002236.1_g000012
rosa_roxburghii Rroxscaffold_5G00380190
rosa_rugosa Rorug04G0317000
rosa_samantha Rh4AG367500 Rh4CG393500 Rh4DG373700
rosa_wichuraiana Rw0G005660 Rw0G011650 Rw5G028530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 369, 1066
AciI CCGC 3 cut(s) 734, 848, 1064
AclWI GGATC 2 cut(s) 89, 1023
AcuI CTGAAG 4 cut(s) 335, 407, 546, 615
AfaI GTAC 1 cut(s) 508
AfiI CCNNNNNNNGG 1 cut(s) 1135
AflII CTTAAG 1 cut(s) 1174
AgsI TTSAA 2 cut(s) 440, 905
AjnI CCWGG 1 cut(s) 378
AluBI AGCT 6 cut(s) 70, 524, 541, 698, 743, 898
AluI AGCT 6 cut(s) 70, 524, 541, 698, 743, 898
Alw21I GWGCWC 1 cut(s) 543
Alw26I GTCTC 3 cut(s) 144, 612, 718
AlwI GGATC 2 cut(s) 89, 1023
AlwNI CAGNNNCTG 2 cut(s) 268, 385
Ama87I CYCGRG 1 cut(s) 215
AoxI GGCC 1 cut(s) 90
ApeKI GCWGC 4 cut(s) 8, 516, 695, 827
ArsI GACNNNNNNTTYG 2 cut(s) 392, 424
AseI ATTAAT 1 cut(s) 647
Asp700I GAANNNNTTC 2 cut(s) 264, 774
AspA2I CCTAGG 1 cut(s) 1184
AspS9I GGNCC 2 cut(s) 90, 449
AsuHPI GGTGA 3 cut(s) 37, 713, 902
AvaI CYCGRG 1 cut(s) 215
AvaII GGWCC 1 cut(s) 449
AvrII CCTAGG 1 cut(s) 1184
AxyI CCTNAGG 1 cut(s) 1119
BanII GRGCYC 1 cut(s) 543
BbsI GAAGAC 1 cut(s) 178
Bbv12I GWGCWC 1 cut(s) 543
BbvI GCAGC 3 cut(s) 503, 707, 814
BccI CCATC 2 cut(s) 475, 875
BceAI ACGGC 1 cut(s) 1149
BciT130I CCWGG 1 cut(s) 380
BciVI GTATCC 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 144, 612, 718
BfaI CTAG 3 cut(s) 99, 947, 1185
BfrI CTTAAG 1 cut(s) 1174
BfuI GTATCC 1 cut(s) 330
BglII AGATCT 1 cut(s) 684
BisI GCNGC 4 cut(s) 9, 517, 696, 828
BlnI CCTAGG 1 cut(s) 1184
BlpI GCTNAGC 1 cut(s) 1160
BlsI GCNGC 4 cut(s) 10, 518, 697, 829
Bme1390I CCNGG 1 cut(s) 380
Bme18I GGWCC 1 cut(s) 449
BmeT110I CYCGRG 1 cut(s) 215
BmgT120I GGNCC 2 cut(s) 90, 449
BmiI GGNNCC 1 cut(s) 450
BmrFI CCNGG 1 cut(s) 380
BmsI GCATC 3 cut(s) 210, 475, 948
BpiI GAAGAC 1 cut(s) 178
Bpu1102I GCTNAGC 1 cut(s) 1160
BpuEI CTTGAG 1 cut(s) 594
BsaI GGTCTC 1 cut(s) 612
BsaJI CCNNGG 4 cut(s) 534, 566, 1165, 1184
Bsc4I CCNNNNNNNGG 1 cut(s) 1135
Bse1I ACTGG 1 cut(s) 855
Bse21I CCTNAGG 1 cut(s) 1119
BseBI CCWGG 1 cut(s) 380
BseDI CCNNGG 4 cut(s) 534, 566, 1165, 1184
BseLI CCNNNNNNNGG 1 cut(s) 1135
BseMII CTCAG 4 cut(s) 137, 373, 513, 1133
BseNI ACTGG 1 cut(s) 855
BseRI GAGGAG 2 cut(s) 551, 1109
BseXI GCAGC 3 cut(s) 503, 707, 814
BsgI GTGCAG 1 cut(s) 502
Bsh1236I CGCG 2 cut(s) 369, 1066
BshFI GGCC 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 543
BsiHKCI CYCGRG 1 cut(s) 215
BslFI GGGAC 5 cut(s) 462, 731, 854, 1006, 1065
BslI CCNNNNNNNGG 1 cut(s) 1135
BsmAI GTCTC 3 cut(s) 144, 612, 718
BsmBI CGTCTC 1 cut(s) 144
BsmFI GGGAC 5 cut(s) 462, 731, 854, 1006, 1065
BsnI GGCC 1 cut(s) 92
Bso31I GGTCTC 1 cut(s) 612
BsoBI CYCGRG 1 cut(s) 215
Bsp1286I GDGCHC 1 cut(s) 543
Bsp143I GATC 5 cut(s) 94, 346, 684, 949, 1028
Bsp1720I GCTNAGC 1 cut(s) 1160
BspACI CCGC 3 cut(s) 734, 848, 1064
BspANI GGCC 1 cut(s) 92
BspCNI CTCAG 4 cut(s) 138, 372, 512, 1132
BspFNI CGCG 2 cut(s) 369, 1066
BspHI TCATGA 1 cut(s) 1036
BspLI GGNNCC 1 cut(s) 450
BspPI GGATC 2 cut(s) 89, 1023
BspTI CTTAAG 1 cut(s) 1174
BspTNI GGTCTC 1 cut(s) 612
BsrI ACTGG 1 cut(s) 855
BssECI CCNNGG 4 cut(s) 534, 566, 1165, 1184
BssMI GATC 5 cut(s) 94, 346, 684, 949, 1028
BssT1I CCWWGG 2 cut(s) 566, 1184
Bst2UI CCWGG 1 cut(s) 380
Bst4CI ACNGT 4 cut(s) 31, 549, 914, 1072
Bst6I CTCTTC 3 cut(s) 591, 596, 780
BstAFI CTTAAG 1 cut(s) 1174
BstC8I GCNNGC 4 cut(s) 6, 284, 492, 657
BstDEI CTNAG 7 cut(s) 146, 359, 470, 499, 737, 1119, 1160
BstDSI CCRYGG 1 cut(s) 1165
BstEII GGTNACC 1 cut(s) 908
BstFNI CGCG 2 cut(s) 369, 1066
BstKTI GATC 5 cut(s) 97, 349, 687, 952, 1031
BstMAI GTCTC 3 cut(s) 144, 612, 718
BstMBI GATC 5 cut(s) 94, 346, 684, 949, 1028
BstMWI GCNNNNNNNGC 2 cut(s) 530, 1063
BstNI CCWGG 1 cut(s) 380
BstNSI RCATGY 2 cut(s) 490, 982
BstPI GGTNACC 1 cut(s) 908
BstSCI CCNGG 1 cut(s) 378
BstUI CGCG 2 cut(s) 369, 1066
BstV1I GCAGC 3 cut(s) 503, 707, 814
BstV2I GAAGAC 1 cut(s) 178
BstX2I RGATCY 2 cut(s) 684, 1028
BstYI RGATCY 2 cut(s) 684, 1028
Bsu36I CCTNAGG 1 cut(s) 1119
BsuI GTATCC 1 cut(s) 330
BsuRI GGCC 1 cut(s) 92
BtgI CCRYGG 1 cut(s) 1165
BtsIMutI CAGTG 2 cut(s) 143, 383
Cac8I GCNNGC 4 cut(s) 6, 284, 492, 657
CaiI CAGNNNCTG 2 cut(s) 268, 385
CciI TCATGA 1 cut(s) 1036
Cfr13I GGNCC 2 cut(s) 90, 449
CseI GACGC 1 cut(s) 358
Csp6I GTAC 1 cut(s) 507
CviAII CATG 7 cut(s) 22, 487, 512, 889, 979, 1037, 1135
CviQI GTAC 1 cut(s) 507
DdeI CTNAG 7 cut(s) 146, 359, 470, 499, 737, 1119, 1160
DpnI GATC 5 cut(s) 96, 348, 686, 951, 1030
DpnII GATC 5 cut(s) 94, 346, 684, 949, 1028
DraI TTTAAA 3 cut(s) 45, 931, 987
Eam1104I CTCTTC 3 cut(s) 591, 596, 780
EarI CTCTTC 3 cut(s) 591, 596, 780
Ecl136II GAGCTC 1 cut(s) 541
Eco130I CCWWGG 2 cut(s) 566, 1184
Eco24I GRGCYC 1 cut(s) 543
Eco31I GGTCTC 1 cut(s) 612
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 541
Eco57I CTGAAG 4 cut(s) 335, 407, 546, 615
Eco81I CCTNAGG 1 cut(s) 1119
Eco88I CYCGRG 1 cut(s) 215
Eco91I GGTNACC 1 cut(s) 908
EcoICRI GAGCTC 1 cut(s) 541
EcoO109I RGGNCCY 1 cut(s) 449
EcoO65I GGTNACC 1 cut(s) 908
EcoRII CCWGG 1 cut(s) 378
EcoT14I CCWWGG 2 cut(s) 566, 1184
EcoT22I ATGCAT 2 cut(s) 562, 984
EcoT38I GRGCYC 1 cut(s) 543
ErhI CCWWGG 2 cut(s) 566, 1184
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 7 cut(s) 25, 490, 515, 892, 982, 1040, 1138
FaqI GGGAC 5 cut(s) 462, 731, 854, 1006, 1065
FatI CATG 7 cut(s) 21, 486, 511, 888, 978, 1036, 1134
Fnu4HI GCNGC 4 cut(s) 9, 517, 696, 828
FriOI GRGCYC 1 cut(s) 543
Fsp4HI GCNGC 4 cut(s) 9, 517, 696, 828
FspBI CTAG 3 cut(s) 99, 947, 1185
GluI GCNGC 4 cut(s) 9, 517, 696, 828
HaeIII GGCC 1 cut(s) 92
HgaI GACGC 1 cut(s) 358
Hin1II CATG 7 cut(s) 25, 490, 515, 892, 982, 1040, 1138
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 5 cut(s) 140, 265, 407, 808, 994
HphI GGTGA 3 cut(s) 37, 713, 902
Hpy166II GTNNAC 2 cut(s) 393, 1181
Hpy188I TCNGA 7 cut(s) 161, 170, 354, 595, 627, 807, 954
Hpy188III TCNNGA 8 cut(s) 65, 217, 257, 344, 440, 457, 1037, 1121
Hpy8I GTNNAC 2 cut(s) 393, 1181
HpyAV CCTTC 2 cut(s) 760, 899
HpyCH4III ACNGT 4 cut(s) 31, 549, 914, 1072
HpyF10VI GCNNNNNNNGC 2 cut(s) 530, 1063
HpyF3I CTNAG 7 cut(s) 146, 359, 470, 499, 737, 1119, 1160
Hsp92II CATG 7 cut(s) 25, 490, 515, 892, 982, 1040, 1138
Kzo9I GATC 5 cut(s) 94, 346, 684, 949, 1028
LmnI GCTCC 1 cut(s) 538
Lsp1109I GCAGC 3 cut(s) 503, 707, 814
LweI GCATC 3 cut(s) 210, 475, 948
MaeI CTAG 3 cut(s) 99, 947, 1185
MaeIII GTNAC 6 cut(s) 17, 25, 416, 457, 908, 1169
MalI GATC 5 cut(s) 96, 348, 686, 951, 1030
MboI GATC 5 cut(s) 94, 346, 684, 949, 1028
MboII GAAGA 7 cut(s) 183, 341, 539, 608, 613, 767, 1056
MfeI CAATTG 1 cut(s) 331
MflI RGATCY 2 cut(s) 684, 1028
MhlI GDGCHC 1 cut(s) 543
MlyI GAGTC 2 cut(s) 416, 988
Mph1103I ATGCAT 2 cut(s) 562, 984
MroXI GAANNNNTTC 2 cut(s) 264, 774
MseI TTAA 9 cut(s) 44, 125, 303, 551, 647, 762, 930, 986, 1175
MslI CAYNNNNRTG 1 cut(s) 987
MspA1I CMGCKG 2 cut(s) 524, 698
MspCI CTTAAG 1 cut(s) 1174
MspR9I CCNGG 1 cut(s) 380
MunI CAATTG 1 cut(s) 331
MvaI CCWGG 1 cut(s) 380
MvnI CGCG 2 cut(s) 369, 1066
MwoI GCNNNNNNNGC 2 cut(s) 530, 1063
NdeII GATC 5 cut(s) 94, 346, 684, 949, 1028
NlaIII CATG 7 cut(s) 25, 490, 515, 892, 982, 1040, 1138
NlaIV GGNNCC 1 cut(s) 450
NmeAIII GCCGAG 2 cut(s) 16, 559
NmuCI GTSAC 4 cut(s) 17, 25, 457, 908
NsiI ATGCAT 2 cut(s) 562, 984
NspI RCATGY 2 cut(s) 490, 982
PaeR7I CTCGAG 1 cut(s) 215
PagI TCATGA 1 cut(s) 1036
PdmI GAANNNNTTC 2 cut(s) 264, 774
PfeI GAWTC 3 cut(s) 140, 265, 808
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 4 cut(s) 10, 518, 697, 829
PleI GAGTC 2 cut(s) 415, 988
PpsI GAGTC 2 cut(s) 415, 988
PpuMI RGGWCCY 1 cut(s) 449
PshBI ATTAAT 1 cut(s) 647
Psp124BI GAGCTC 1 cut(s) 543
Psp5II RGGWCCY 1 cut(s) 449
Psp6I CCWGG 1 cut(s) 378
PspEI GGTNACC 1 cut(s) 908
PspGI CCWGG 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 450
PspPI GGNCC 2 cut(s) 90, 449
PspPPI RGGWCCY 1 cut(s) 449
PsrI GAACNNNNNNTAC 2 cut(s) 1017, 1049
PstNI CAGNNNCTG 2 cut(s) 268, 385
PsuI RGATCY 2 cut(s) 684, 1028
PsyI GACNNNGTC 1 cut(s) 155
PvuII CAGCTG 2 cut(s) 524, 698
RsaI GTAC 1 cut(s) 508
RsaNI GTAC 1 cut(s) 507
RseI CAYNNNNRTG 1 cut(s) 987
SacI GAGCTC 1 cut(s) 543
SaqAI TTAA 9 cut(s) 44, 125, 303, 551, 647, 762, 930, 986, 1175
SatI GCNGC 4 cut(s) 9, 517, 696, 828
Sau3AI GATC 5 cut(s) 94, 346, 684, 949, 1028
Sau96I GGNCC 2 cut(s) 90, 449
SchI GAGTC 2 cut(s) 416, 988
ScrFI CCNGG 1 cut(s) 380
SduI GDGCHC 1 cut(s) 543
SfaNI GCATC 3 cut(s) 210, 475, 948
Sfr274I CTCGAG 1 cut(s) 215
SinI GGWCC 1 cut(s) 449
SlaI CTCGAG 1 cut(s) 215
SmiI ATTTAAAT 1 cut(s) 987
SmiMI CAYNNNNRTG 1 cut(s) 987
SmlI CTYRAG 3 cut(s) 215, 609, 1174
SmoI CTYRAG 3 cut(s) 215, 609, 1174
SsiI CCGC 3 cut(s) 734, 848, 1064
SspMI CTAG 3 cut(s) 99, 947, 1185
SstI GAGCTC 1 cut(s) 543
StyD4I CCNGG 1 cut(s) 378
StyI CCWWGG 2 cut(s) 566, 1184
SwaI ATTTAAAT 1 cut(s) 987
TaaI ACNGT 4 cut(s) 31, 549, 914, 1072
TaqI TCGA 3 cut(s) 216, 258, 966
TatI WGTACW 1 cut(s) 506
TfiI GAWTC 3 cut(s) 140, 265, 808
Tru1I TTAA 9 cut(s) 44, 125, 303, 551, 647, 762, 930, 986, 1175
Tru9I TTAA 9 cut(s) 44, 125, 303, 551, 647, 762, 930, 986, 1175
TscAI CASTG 2 cut(s) 150, 390
TseFI GTSAC 4 cut(s) 17, 25, 457, 908
TseI GCWGC 4 cut(s) 8, 516, 695, 827
Tsp45I GTSAC 4 cut(s) 17, 25, 457, 908
TspDTI ATGAA 4 cut(s) 124, 258, 804, 1025
TspGWI ACGGA 1 cut(s) 1021
TspRI CASTG 2 cut(s) 150, 390
Tth111I GACNNNGTC 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 1174
VpaK11BI GGWCC 1 cut(s) 449
VspI ATTAAT 1 cut(s) 647
XceI RCATGY 2 cut(s) 490, 982
XhoI CTCGAG 1 cut(s) 215
XmaJI CCTAGG 1 cut(s) 1184
XmnI GAANNNNTTC 2 cut(s) 264, 774
XspI CTAG 3 cut(s) 99, 947, 1185
Zsp2I ATGCAT 2 cut(s) 562, 984
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.