Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0008476

Physical Location & Seq

Genomic Coordinates Forward (+)

21911499 .. 21915506

Gene Structure 4008 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1104300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1425 bp

ATGGAGAACGCCGACGTGTTCTTAGGACTGCACGACTTTCTGGAACGCATGCGTCAACCGTCGGCCGCTGATTTCGTCAAGTCCATAAAAAGTTTCATAGTGTCGTTTACGAACAATGCTCCTGATCCTGAGGGGGACAGCGCTGCAGTGCAGGACTTCTTTGCCCGGATGGAAATTGATTTCAGGGCTCATCCACTTTGGGCGGGCTGTTCAGAGGAGGAGCTCGACAGTGCCGGCGAAGGACTGGAGAAGTATGTTATGACGAAGTTGTTTCCTCGCGTATTTGCTTCGCTTCCAGATGATGTAAAACTTGATGAGCAACTGTATGAGAAGATGGCTTTAGTACAACAGTTTATTCGGCCGGAAAATTTGGATATCAAGCCCACCTTTCAAAATGAGACTTCATGGCTGCTTGCACAAAAAGAACTGCAAAAGATCAATATGTATAAGGCACCGAGGGATAAGCTTGTGTGTATCCTCAATTGTTGCAAGGTTATCAATAACTTGCTGATGAATGCTTCTGTTGCTTCCAATGAGAATCCCCCTGGAGCTGATGAATTTCTTCCAGTCCTAATTTATGTTACTTTAAAGGCAAACCCTCCACAACTGCACTCAAATTTATTGTATATACAAAGATACAGGCGACAATCTCGATTAGTTGGTGAAGCATCCTATTTTTTCACAAATATGCTCTCTGTTGAGTCCTTCATCTCAAATATAGATGCAAATGCTATTTCACTGGATGAATCTGAGTACGACAAGAACATGGAAACTGCTCGAGCACTTCTTTCAGGACTTTCAACTGATTTGGATGGTCAGTATACTCAAAGCAGTCCATTTGCAGGATATGTACATAGATCAGAATCTATGGAAACCAGACATCAACCTCCAAATGCCAACAAGGACCCAGCAGCACAGTCCAAATCTTCAGAAACAAAATCCCGAGGGAAGAAGATACCCAATGCAAAGGATAACTCACCCTATTCAAAAATTCCATCTCTTTCAGATTTGGAGAATAAGGGTGCAGCTGTCCTTGTAAACAACGATCAGGCGGGCCAGGTTTTCCAGGAATATCCATACTTGTTTGTGAATGTTGGGGACCTAACCATCAATGATGTAGAGGACCTTCTTAATAATTACAAGCAACTTGCATTCAAATATGTTTGTCTTTCCAAAGGGTTAGGCGTTACTGCTCCATCTCTTCCTTACAATTTGCATACAGAGTTCCACCAGCATACTGAAACGGCAAAGCAACAGGAACGCTCTAGAGCTGTAGAGCAGAATGATGAGACGTCAGATGACACTGATAAGAAGGATGATGGATTAAAGGAGCCCTTTATTGATGTCGAAAACTTGGAATCTAGATTGTCACAGGATCGAGCCGCTGCACCACAGAGTGGTTCACATGAGGAGTCCTCTCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000919 GO:0003006 GO:0003674 GO:0005085 GO:0005088 GO:0005089 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0007049 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009987 GO:0010154 GO:0016020 GO:0016043 GO:0017016 GO:0017048 GO:0019899 GO:0022402 GO:0022414 GO:0022607 GO:0022622 GO:0031267 GO:0032501 GO:0032502 GO:0032506 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044085 GO:0044424 GO:0044444 GO:0044464 GO:0048316 GO:0048364 GO:0048528 GO:0048608 GO:0048731 GO:0048856 GO:0051020 GO:0051301 GO:0061458 GO:0061640 GO:0065007 GO:0065009 GO:0071554 GO:0071840 GO:0090696 GO:0098772 GO:0099402 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

VPS9

Protein Analysis

475

Amino Acids

53.29

Weight (kDa)

4.87

Isoelectric Point (pI)

41.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF5601	PF18151	27 - 91	2.9e-14	Domain of unknown function (DUF5601)
VPS9	PF02204	137 - 241	5.6e-31	Vacuolar sorting protein 9 (VPS9) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G19770 AT3G19770 AT5G09320
fragaria_vesca	FvH4_5g38820 FvH4_5g38820
malus_domestica	MD00G1104300.v1.1 MD15G1358700.v1.1
prunus_persica	Prupe.1G506400_v2.0.a1 Prupe.1G506400_v2.0.a1
pyrus_communis	pycom08g14820 pycom15g32120
rosa_chinensis	RchiOBHm_Chr7g0240821
rosa_laevigata	RLG00000000622
rosa_multiflora	Rmu_sc0004821.1_g000014 Rmu_sc0010888.1_g000004
rosa_roxburghii	Rroxscaffold_3G00219600
rosa_rugosa	Rorug07G0339300 Rorug07G0339300
rosa_samantha	Rh7AG493500 Rh7BG465500 Rh7CG512000 Rh7DG478700
rosa_wichuraiana	Rw7G041760

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1295
AccB1I	GGYRCC	1 cut(s)	451
AccB7I	CCANNNNNTGG	1 cut(s)	1397
AccI	GTMKAC	1 cut(s)	821
AccII	CGCG	1 cut(s)	279
AciI	CCGC	4 cut(s)	66, 203, 1052, 1383
AclWI	GGATC	2 cut(s)	119, 1383
AcoI	YGGCCR	2 cut(s)	63, 359
AcsI	RAATTY	4 cut(s)	367, 557, 616, 990
AcuI	CTGAAG	1 cut(s)	912
AcyI	GRCGYC	1 cut(s)	1292
AdeI	CACNNNGTG	1 cut(s)	1397
AfaI	GTAC	3 cut(s)	345, 755, 852
AfeI	AGCGCT	1 cut(s)	142
AfiI	CCNNNNNNNGG	2 cut(s)	842, 1397
AflIII	ACRYGT	1 cut(s)	15
AgsI	TTSAA	4 cut(s)	392, 801, 987, 1156
AjiI	CACGTC	1 cut(s)	16
AjnI	CCWGG	3 cut(s)	544, 1056, 1065
AluBI	AGCT	5 cut(s)	223, 466, 551, 1028, 1271
AluI	AGCT	5 cut(s)	223, 466, 551, 1028, 1271
Alw21I	GWGCWC	2 cut(s)	225, 784
Alw26I	GTCTC	2 cut(s)	392, 1283
AlwI	GGATC	2 cut(s)	119, 1383
Ama87I	CYCGRG	2 cut(s)	777, 942
Aor51HI	AGCGCT	1 cut(s)	142
AoxI	GGCC	3 cut(s)	63, 359, 1054
ApeKI	GCWGC	5 cut(s)	143, 409, 911, 1025, 1385
ApoI	RAATTY	4 cut(s)	367, 557, 616, 990
Asp700I	GAANNNNTTC	1 cut(s)	561
AspLEI	GCGC	1 cut(s)	143
AspS9I	GGNCC	4 cut(s)	904, 1054, 1099, 1123
AsuC2I	CCSGG	1 cut(s)	166
AsuHPI	GGTGA	2 cut(s)	674, 969
AvaI	CYCGRG	2 cut(s)	777, 942
AvaII	GGWCC	3 cut(s)	904, 1099, 1123
AxyI	CCTNAGG	1 cut(s)	129
BanI	GGYRCC	1 cut(s)	451
BanII	GRGCYC	3 cut(s)	190, 225, 1335
BarI	GAAGNNNNNNTAC	2 cut(s)	1110, 1142
Bbv12I	GWGCWC	2 cut(s)	225, 784
BbvI	GCAGC	5 cut(s)	130, 396, 923, 1037, 1372
BccI	CCATC	7 cut(s)	163, 328, 806, 1003, 1115, 1204, 1313
BceAI	ACGGC	1 cut(s)	1260
BciT130I	CCWGG	3 cut(s)	546, 1058, 1067
BciVI	GTATCC	1 cut(s)	485
BcnI	CCSGG	1 cut(s)	166
BcoDI	GTCTC	2 cut(s)	392, 1283
BfaI	CTAG	2 cut(s)	1266, 1362
BfmI	CTRYAG	2 cut(s)	144, 1272
BfoI	RGCGCY	1 cut(s)	144
BfuI	GTATCC	1 cut(s)	485
BisI	GCNGC	7 cut(s)	66, 144, 410, 912, 1026, 1383, 1386
BlsI	GCNGC	7 cut(s)	67, 145, 411, 913, 1027, 1384, 1387
Bme1390I	CCNGG	4 cut(s)	166, 546, 1058, 1067
Bme18I	GGWCC	3 cut(s)	904, 1099, 1123
BmeT110I	CYCGRG	2 cut(s)	777, 942
BmgBI	CACGTC	1 cut(s)	16
BmgT120I	GGNCC	4 cut(s)	904, 1054, 1099, 1123
BmiI	GGNNCC	4 cut(s)	453, 906, 1100, 1332
BmrFI	CCNGG	4 cut(s)	166, 546, 1058, 1067
BmsI	GCATC	2 cut(s)	677, 712
BplI	GAGNNNNNCTC	1 cut(s)	1400
BpmI	CTGGAG	2 cut(s)	266, 567
BpuMI	CCSGG	1 cut(s)	166
BsaBI	GATNNNNATC	1 cut(s)	862
BsaHI	GRCGYC	1 cut(s)	1292
BsaJI	CCNNGG	3 cut(s)	455, 544, 943
Bsc4I	CCNNNNNNNGG	2 cut(s)	842, 1397
Bse118I	RCCGGY	1 cut(s)	233
Bse1I	ACTGG	3 cut(s)	249, 566, 744
Bse21I	CCTNAGG	1 cut(s)	129
Bse8I	GATNNNNATC	1 cut(s)	862
BseBI	CCWGG	3 cut(s)	546, 1058, 1067
BseDI	CCNNGG	3 cut(s)	455, 544, 943
BseGI	GGATG	6 cut(s)	174, 190, 668, 748, 817, 1321
BseJI	GATNNNNATC	1 cut(s)	862
BseLI	CCNNNNNNNGG	2 cut(s)	842, 1397
BseMII	CTCAG	2 cut(s)	120, 741
BseNI	ACTGG	3 cut(s)	249, 566, 744
BseRI	GAGGAG	3 cut(s)	230, 233, 1424
BseX3I	CGGCCG	2 cut(s)	63, 359
BseXI	GCAGC	5 cut(s)	130, 396, 923, 1037, 1372
BseYI	CCCAGC	1 cut(s)	907
BsgI	GTGCAG	5 cut(s)	14, 170, 593, 1044, 1371
Bsh1236I	CGCG	1 cut(s)	279
Bsh1285I	CGRYCG	2 cut(s)	66, 362
BshFI	GGCC	3 cut(s)	65, 361, 1056
BshNI	GGYRCC	1 cut(s)	451
BsiEI	CGRYCG	2 cut(s)	66, 362
BsiHKAI	GWGCWC	2 cut(s)	225, 784
BsiHKCI	CYCGRG	2 cut(s)	777, 942
BsiSI	CCGG	3 cut(s)	166, 234, 362
BslFI	GGGAC	2 cut(s)	149, 1112
BslI	CCNNNNNNNGG	2 cut(s)	842, 1397
BsmAI	GTCTC	2 cut(s)	392, 1283
BsmBI	CGTCTC	1 cut(s)	1283
BsmFI	GGGAC	2 cut(s)	149, 1112
BsmI	GAATGC	2 cut(s)	520, 1151
BsnI	GGCC	3 cut(s)	65, 361, 1056
BsoBI	CYCGRG	2 cut(s)	777, 942
Bsp1286I	GDGCHC	4 cut(s)	190, 225, 784, 1335
Bsp1407I	TGTACA	1 cut(s)	850
Bsp143I	GATC	5 cut(s)	124, 435, 857, 1045, 1375
BspACI	CCGC	4 cut(s)	66, 203, 1052, 1383
BspANI	GGCC	3 cut(s)	65, 361, 1056
BspCNI	CTCAG	2 cut(s)	121, 742
BspFNI	CGCG	1 cut(s)	279
BspLI	GGNNCC	4 cut(s)	453, 906, 1100, 1332
BspMAI	CTGCAG	1 cut(s)	148
BspPI	GGATC	2 cut(s)	119, 1383
BspT107I	GGYRCC	1 cut(s)	451
BsrFI	RCCGGY	1 cut(s)	233
BsrGI	TGTACA	1 cut(s)	850
BsrI	ACTGG	3 cut(s)	249, 566, 744
BssAI	RCCGGY	1 cut(s)	233
BssECI	CCNNGG	3 cut(s)	455, 544, 943
BssMI	GATC	5 cut(s)	124, 435, 857, 1045, 1375
BssNAI	GTATAC	1 cut(s)	822
BssNI	GRCGYC	1 cut(s)	1292
Bst1107I	GTATAC	1 cut(s)	822
Bst2UI	CCWGG	3 cut(s)	546, 1058, 1067
Bst4CI	ACNGT	5 cut(s)	60, 230, 324, 351, 918
Bst6I	CTCTTC	1 cut(s)	1206
BstACI	GRCGYC	1 cut(s)	1292
BstAUI	TGTACA	1 cut(s)	850
BstC8I	GCNNGC	5 cut(s)	50, 205, 235, 414, 1054
BstDEI	CTNAG	4 cut(s)	22, 129, 750, 1419
BstF5I	GGATG	6 cut(s)	174, 190, 668, 748, 817, 1321
BstFNI	CGCG	1 cut(s)	279
BstH2I	RGCGCY	1 cut(s)	144
BstHHI	GCGC	1 cut(s)	143
BstKTI	GATC	5 cut(s)	127, 438, 860, 1048, 1378
BstMAI	GTCTC	2 cut(s)	392, 1283
BstMBI	GATC	5 cut(s)	124, 435, 857, 1045, 1375
BstMCI	CGRYCG	2 cut(s)	66, 362
BstMWI	GCNNNNNNNGC	1 cut(s)	524
BstNI	CCWGG	3 cut(s)	546, 1058, 1067
BstNSI	RCATGY	1 cut(s)	52
BstSCI	CCNGG	4 cut(s)	164, 544, 1056, 1065
BstSFI	CTRYAG	2 cut(s)	144, 1272
BstUI	CGCG	1 cut(s)	279
BstV1I	GCAGC	5 cut(s)	130, 396, 923, 1037, 1372
BstZ17I	GTATAC	1 cut(s)	822
BstZI	CGGCCG	2 cut(s)	63, 359
Bsu36I	CCTNAGG	1 cut(s)	129
BsuI	GTATCC	1 cut(s)	485
BsuRI	GGCC	3 cut(s)	65, 361, 1056
BtrI	CACGTC	1 cut(s)	16
BtsCI	GGATG	6 cut(s)	174, 190, 668, 748, 817, 1321
BtsI	GCAGTG	1 cut(s)	153
BtsIMutI	CAGTG	4 cut(s)	153, 235, 737, 1302
Cac8I	GCNNGC	5 cut(s)	50, 205, 235, 414, 1054
CfoI	GCGC	1 cut(s)	143
Cfr10I	RCCGGY	1 cut(s)	233
Cfr13I	GGNCC	4 cut(s)	904, 1054, 1099, 1123
CseI	GACGC	1 cut(s)	41
Csp6I	GTAC	3 cut(s)	344, 754, 851
CviAII	CATG	4 cut(s)	49, 405, 766, 1406
CviQI	GTAC	3 cut(s)	344, 754, 851
DdeI	CTNAG	4 cut(s)	22, 129, 750, 1419
DpnI	GATC	5 cut(s)	126, 437, 859, 1047, 1377
DpnII	GATC	5 cut(s)	124, 435, 857, 1045, 1375
DraI	TTTAAA	1 cut(s)	588
DraIII	CACNNNGTG	1 cut(s)	1397
EaeI	YGGCCR	2 cut(s)	63, 359
EagI	CGGCCG	2 cut(s)	63, 359
Eam1104I	CTCTTC	1 cut(s)	1206
EarI	CTCTTC	1 cut(s)	1206
Ecl136II	GAGCTC	1 cut(s)	223
EclXI	CGGCCG	2 cut(s)	63, 359
Eco24I	GRGCYC	3 cut(s)	190, 225, 1335
Eco32I	GATATC	1 cut(s)	376
Eco47I	GGWCC	3 cut(s)	904, 1099, 1123
Eco47III	AGCGCT	1 cut(s)	142
Eco52I	CGGCCG	2 cut(s)	63, 359
Eco53kI	GAGCTC	1 cut(s)	223
Eco57I	CTGAAG	1 cut(s)	912
Eco81I	CCTNAGG	1 cut(s)	129
Eco88I	CYCGRG	2 cut(s)	777, 942
EcoICRI	GAGCTC	1 cut(s)	223
EcoO109I	RGGNCCY	3 cut(s)	904, 1099, 1123
EcoRII	CCWGG	3 cut(s)	544, 1056, 1065
EcoRV	GATATC	1 cut(s)	376
EcoT38I	GRGCYC	3 cut(s)	190, 225, 1335
Esp3I	CGTCTC	1 cut(s)	1283
FaeI	CATG	4 cut(s)	52, 408, 769, 1409
FalI	AAGNNNNNCTT	4 cut(s)	371, 403, 1319, 1351
FaqI	GGGAC	2 cut(s)	149, 1112
FatI	CATG	4 cut(s)	48, 404, 765, 1405
FauI	CCCGC	2 cut(s)	196, 1045
FblI	GTMKAC	1 cut(s)	821
Fnu4HI	GCNGC	7 cut(s)	66, 144, 410, 912, 1026, 1383, 1386
FokI	GGATG	6 cut(s)	177, 181, 655, 755, 824, 1328
FriOI	GRGCYC	3 cut(s)	190, 225, 1335
Fsp4HI	GCNGC	7 cut(s)	66, 144, 410, 912, 1026, 1383, 1386
FspBI	CTAG	2 cut(s)	1266, 1362
GlaI	GCGC	1 cut(s)	142
GluI	GCNGC	7 cut(s)	66, 144, 410, 912, 1026, 1383, 1386
GsaI	CCCAGC	1 cut(s)	911
GsuI	CTGGAG	2 cut(s)	266, 567
HaeII	RGCGCY	1 cut(s)	144
HaeIII	GGCC	3 cut(s)	65, 361, 1056
HapII	CCGG	3 cut(s)	166, 234, 362
HgaI	GACGC	1 cut(s)	41
HhaI	GCGC	1 cut(s)	143
Hin1I	GRCGYC	1 cut(s)	1292
Hin1II	CATG	4 cut(s)	52, 408, 769, 1409
Hin6I	GCGC	1 cut(s)	141
HinP1I	GCGC	1 cut(s)	141
HincII	GTYRAC	1 cut(s)	56
HindII	GTYRAC	1 cut(s)	56
HindIII	AAGCTT	1 cut(s)	464
HinfI	GANTC	6 cut(s)	538, 701, 746, 863, 1358, 1412
HpaII	CCGG	3 cut(s)	166, 234, 362
HphI	GGTGA	2 cut(s)	674, 969
Hpy166II	GTNNAC	5 cut(s)	56, 108, 822, 1039, 1403
Hpy188I	TCNGA	6 cut(s)	214, 751, 862, 931, 1006, 1297
Hpy188III	TCNNGA	9 cut(s)	41, 122, 128, 296, 651, 792, 942, 1266, 1362
Hpy8I	GTNNAC	5 cut(s)	56, 108, 822, 1039, 1403
Hpy99I	CGWCG	2 cut(s)	17, 64
HpyAV	CCTTC	4 cut(s)	233, 715, 1136, 1306
HpyCH4III	ACNGT	5 cut(s)	60, 230, 324, 351, 918
HpyCH4IV	ACGT	2 cut(s)	15, 1292
HpyF10VI	GCNNNNNNNGC	1 cut(s)	524
HpyF3I	CTNAG	4 cut(s)	22, 129, 750, 1419
HpySE526I	ACGT	2 cut(s)	15, 1292
Hsp92I	GRCGYC	1 cut(s)	1292
Hsp92II	CATG	4 cut(s)	52, 408, 769, 1409
HspAI	GCGC	1 cut(s)	141
KroI	GCCGGC	1 cut(s)	233
KroNI	GCCGGC	1 cut(s)	235
Kzo9I	GATC	5 cut(s)	124, 435, 857, 1045, 1375
LmnI	GCTCC	5 cut(s)	124, 220, 548, 1198, 1330
Lsp1109I	GCAGC	5 cut(s)	130, 396, 923, 1037, 1372
LweI	GCATC	2 cut(s)	677, 712
MaeI	CTAG	2 cut(s)	1266, 1362
MaeII	ACGT	2 cut(s)	15, 1292
MaeIII	GTNAC	3 cut(s)	580, 1186, 1368
MalI	GATC	5 cut(s)	126, 437, 859, 1047, 1377
MboI	GATC	5 cut(s)	124, 435, 857, 1045, 1375
MboII	GAAGA	6 cut(s)	343, 554, 918, 961, 964, 1193
MfeI	CAATTG	1 cut(s)	481
MhlI	GDGCHC	4 cut(s)	190, 225, 784, 1335
MluCI	AATT	9 cut(s)	174, 367, 481, 557, 573, 616, 990, 1135, 1210
MlyI	GAGTC	2 cut(s)	710, 1421
MroNI	GCCGGC	1 cut(s)	233
MroXI	GAANNNNTTC	1 cut(s)	561
MseI	TTAA	3 cut(s)	587, 1131, 1325
MslI	CAYNNNNRTG	1 cut(s)	686
MspA1I	CMGCKG	3 cut(s)	68, 1028, 1385
MspI	CCGG	3 cut(s)	166, 234, 362
MspR9I	CCNGG	4 cut(s)	166, 546, 1058, 1067
MunI	CAATTG	1 cut(s)	481
Mva1269I	GAATGC	2 cut(s)	520, 1151
MvaI	CCWGG	3 cut(s)	546, 1058, 1067
MvnI	CGCG	1 cut(s)	279
MwoI	GCNNNNNNNGC	1 cut(s)	524
NaeI	GCCGGC	1 cut(s)	235
NciI	CCSGG	1 cut(s)	166
NdeII	GATC	5 cut(s)	124, 435, 857, 1045, 1375
NgoMIV	GCCGGC	1 cut(s)	233
NlaIII	CATG	4 cut(s)	52, 408, 769, 1409
NlaIV	GGNNCC	4 cut(s)	453, 906, 1100, 1332
NmuCI	GTSAC	1 cut(s)	1368
NspI	RCATGY	1 cut(s)	52
PaeI	GCATGC	1 cut(s)	52
PaeR7I	CTCGAG	1 cut(s)	777
PctI	GAATGC	2 cut(s)	520, 1151
PdiI	GCCGGC	1 cut(s)	235
PdmI	GAANNNNTTC	1 cut(s)	561
PfeI	GAWTC	4 cut(s)	538, 746, 863, 1358
PflMI	CCANNNNNTGG	1 cut(s)	1397
PfoI	TCCNGGA	1 cut(s)	1065
PkrI	GCNGC	7 cut(s)	67, 145, 411, 913, 1027, 1384, 1387
PleI	GAGTC	2 cut(s)	709, 1420
PpsI	GAGTC	2 cut(s)	709, 1420
PpuMI	RGGWCCY	3 cut(s)	904, 1099, 1123
Psp124BI	GAGCTC	1 cut(s)	225
Psp5II	RGGWCCY	3 cut(s)	904, 1099, 1123
Psp6I	CCWGG	3 cut(s)	544, 1056, 1065
PspFI	CCCAGC	1 cut(s)	907
PspGI	CCWGG	3 cut(s)	544, 1056, 1065
PspN4I	GGNNCC	4 cut(s)	453, 906, 1100, 1332
PspPI	GGNCC	4 cut(s)	904, 1054, 1099, 1123
PspPPI	RGGWCCY	3 cut(s)	904, 1099, 1123
PspXI	VCTCGAGB	1 cut(s)	777
PstI	CTGCAG	1 cut(s)	148
PvuII	CAGCTG	1 cut(s)	1028
RsaI	GTAC	3 cut(s)	345, 755, 852
RsaNI	GTAC	3 cut(s)	344, 754, 851
RseI	CAYNNNNRTG	1 cut(s)	686
SacI	GAGCTC	1 cut(s)	225
SaqAI	TTAA	3 cut(s)	587, 1131, 1325
SatI	GCNGC	7 cut(s)	66, 144, 410, 912, 1026, 1383, 1386
Sau3AI	GATC	5 cut(s)	124, 435, 857, 1045, 1375
Sau96I	GGNCC	4 cut(s)	904, 1054, 1099, 1123
SchI	GAGTC	2 cut(s)	710, 1421
ScrFI	CCNGG	4 cut(s)	166, 546, 1058, 1067
SduI	GDGCHC	4 cut(s)	190, 225, 784, 1335
SfaNI	GCATC	2 cut(s)	677, 712
SfcI	CTRYAG	2 cut(s)	144, 1272
Sfr274I	CTCGAG	1 cut(s)	777
SinI	GGWCC	3 cut(s)	904, 1099, 1123
SlaI	CTCGAG	1 cut(s)	777
SmiMI	CAYNNNNRTG	1 cut(s)	686
SmlI	CTYRAG	1 cut(s)	777
SmoI	CTYRAG	1 cut(s)	777
SphI	GCATGC	1 cut(s)	52
Sse9I	AATT	9 cut(s)	174, 367, 481, 557, 573, 616, 990, 1135, 1210
SsiI	CCGC	4 cut(s)	66, 203, 1052, 1383
SspMI	CTAG	2 cut(s)	1266, 1362
SstI	GAGCTC	1 cut(s)	225
StyD4I	CCNGG	4 cut(s)	164, 544, 1056, 1065
TaaI	ACNGT	5 cut(s)	60, 230, 324, 351, 918
TaiI	ACGT	2 cut(s)	18, 1295
TaqI	TCGA	5 cut(s)	225, 652, 778, 1347, 1378
TasI	AATT	9 cut(s)	174, 367, 481, 557, 573, 616, 990, 1135, 1210
TatI	WGTACW	2 cut(s)	343, 850
TauI	GCSGC	2 cut(s)	68, 1385
TfiI	GAWTC	4 cut(s)	538, 746, 863, 1358
Tru1I	TTAA	3 cut(s)	587, 1131, 1325
Tru9I	TTAA	3 cut(s)	587, 1131, 1325
TscAI	CASTG	4 cut(s)	153, 235, 744, 1309
TseFI	GTSAC	1 cut(s)	1368
TseI	GCWGC	5 cut(s)	143, 409, 911, 1025, 1385
Tsp45I	GTSAC	1 cut(s)	1368
TspDTI	ATGAA	6 cut(s)	85, 393, 527, 570, 697, 759
TspRI	CASTG	4 cut(s)	153, 235, 744, 1309
Van91I	CCANNNNNTGG	1 cut(s)	1397
VpaK11BI	GGWCC	3 cut(s)	904, 1099, 1123
XapI	RAATTY	4 cut(s)	367, 557, 616, 990
XbaI	TCTAGA	2 cut(s)	1265, 1361
XceI	RCATGY	1 cut(s)	52
XhoI	CTCGAG	1 cut(s)	777
XmiI	GTMKAC	1 cut(s)	821
XmnI	GAANNNNTTC	1 cut(s)	561
XspI	CTAG	2 cut(s)	1266, 1362
ZraI	GACGTC	1 cut(s)	1293

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.