MD00G1128800.v1.1

YLS9-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017146
Physical Location & Seq
Reverse (-)
27419311 .. 27420067
757 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1128800.v1.1.491

Sequence Viewer

Length: 621 bp
ATGAGTGAAACAATCACCGCGAGCGACAGCAGTGGCAGCCGCCGCCAGCTCTACATCTACCTCGCTTGGGGAACTGGCATCTTTGTTGTCCTCTTCGCCGTTGTAGTCTTGCTTGTTTACCTCATTCTCAGGCCCTGCCGGCCAAGCTTCATTCTCCAAGACATGACTGTTTACAACTTCAACTCTTCCACCGCACCACCCTTCGCTCTCACCACCAAGATGCAGGTGACACTCTCCTCTACAAACCCTAATTCTAGGGTTGGCATAGCGTACCAATCCGGCATGGACATCTACGCCGTGTACCGAGACCAGCAGATCACGTTGGCATCTCTCTTGCCTGGAACGTACCAAGGGCACAAAGAGCCTGTGGTGTGGTCGCCGCTTTTGAATGGCGATAGCGTGCCGATATCTCCATTGTCCGGGGAGGCCTTTGCACAAGATCTGAATGCTGGGACGGTGATGATAAAAATCAAGCTTGACGGGAAGGTGATGTGGAAGGTGGGCACTTGGATTTCGGGCAATTATCACTTGAACGTGCTGTGCACGGGTAATATCAAGTTTGGTGATATGATCATCAAGGGTCAGCCGACGGATCCTTGCATTGTGGAAATTGGTCTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LEA_2

Protein Analysis

207

Amino Acids

22.54

Weight (kDa)

7.68

Isoelectric Point (pI)

38.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LEA_2 PF03168 77 - 182 1.5e-06 Late embryogenesis abundant protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017146)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52470
fragaria_vesca FvH4_1g09370
malus_domestica MD00G1128800.v1.1
rosa_chinensis RchiOBHm_Chr2g0095911
rosa_laevigata RLG00000016606
rosa_multiflora Rmu_co8128420.1_g000001
rosa_roxburghii Rroxscaffold_2G00145970
rosa_rugosa Rorug02G0053900
rosa_samantha Rh2AG103000 Rh2BG104400 Rh2CG106900 Rh2DG104600
rosa_wichuraiana Rw2G007830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 214
Acc36I ACCTGC 1 cut(s) 214
AccII CGCG 1 cut(s) 20
AciI CCGC 5 cut(s) 18, 40, 43, 192, 380
AclWI GGATC 2 cut(s) 587, 600
AcoI YGGCCR 1 cut(s) 140
AfaI GTAC 3 cut(s) 272, 302, 347
AfiI CCNNNNNNNGG 2 cut(s) 67, 419
AgsI TTSAA 3 cut(s) 181, 388, 532
AjnI CCWGG 1 cut(s) 337
AluBI AGCT 3 cut(s) 49, 147, 475
AluI AGCT 3 cut(s) 49, 147, 475
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 300
Alw44I GTGCAC 1 cut(s) 541
AlwI GGATC 2 cut(s) 587, 600
AlwNI CAGNNNCTG 1 cut(s) 135
AoxI GGCC 3 cut(s) 131, 140, 426
ApaLI GTGCAC 1 cut(s) 541
ApeKI GCWGC 1 cut(s) 36
AspS9I GGNCC 1 cut(s) 132
AsuC2I CCSGG 1 cut(s) 421
AsuHPI GGTGA 6 cut(s) 7, 202, 238, 469, 499, 575
BaeGI GKGCMC 3 cut(s) 357, 506, 545
BamHI GGATCC 1 cut(s) 592
Bbv12I GWGCWC 1 cut(s) 545
BbvI GCAGC 1 cut(s) 48
BceAI ACGGC 2 cut(s) 83, 281
BciT130I CCWGG 1 cut(s) 339
BclI TGATCA 1 cut(s) 570
BcnI CCSGG 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 300
BfaI CTAG 1 cut(s) 255
BfuAI ACCTGC 1 cut(s) 214
BglI GCCNNNNNGGC 1 cut(s) 139
BglII AGATCT 1 cut(s) 439
BisI GCNGC 4 cut(s) 37, 40, 43, 380
BlsI GCNGC 4 cut(s) 38, 41, 44, 381
Bme1390I CCNGG 2 cut(s) 339, 421
BmgT120I GGNCC 1 cut(s) 132
BmiI GGNNCC 1 cut(s) 594
BmrFI CCNGG 2 cut(s) 339, 421
BmsI GCATC 3 cut(s) 87, 210, 335
BpuMI CCSGG 1 cut(s) 421
BsaBI GATNNNNATC 1 cut(s) 467
BsaI GGTCTC 1 cut(s) 300
BsaJI CCNNGG 2 cut(s) 349, 420
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 419
Bse118I RCCGGY 1 cut(s) 138
Bse1I ACTGG 1 cut(s) 79
Bse8I GATNNNNATC 1 cut(s) 467
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 349, 420
BseJI GATNNNNATC 1 cut(s) 467
BseLI CCNNNNNNNGG 2 cut(s) 67, 419
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 79
BseRI GAGGAG 1 cut(s) 226
BseSI GKGCMC 3 cut(s) 357, 506, 545
BseXI GCAGC 1 cut(s) 48
BseYI CCCAGC 1 cut(s) 449
Bsh1236I CGCG 1 cut(s) 20
BshFI GGCC 3 cut(s) 133, 142, 428
BsiHKAI GWGCWC 1 cut(s) 545
BsiSI CCGG 3 cut(s) 139, 279, 420
BslFI GGGAC 1 cut(s) 466
BslI CCNNNNNNNGG 2 cut(s) 67, 419
BsmAI GTCTC 1 cut(s) 300
BsmFI GGGAC 1 cut(s) 466
BsmI GAATGC 1 cut(s) 451
BsnI GGCC 3 cut(s) 133, 142, 428
Bso31I GGTCTC 1 cut(s) 300
Bsp1286I GDGCHC 3 cut(s) 357, 506, 545
Bsp143I GATC 4 cut(s) 315, 439, 570, 592
BspACI CCGC 5 cut(s) 18, 40, 43, 192, 380
BspANI GGCC 3 cut(s) 133, 142, 428
BspCNI CTCAG 1 cut(s) 141
BspFNI CGCG 1 cut(s) 20
BspLI GGNNCC 1 cut(s) 594
BspMI ACCTGC 1 cut(s) 214
BspPI GGATC 2 cut(s) 587, 600
BspTNI GGTCTC 1 cut(s) 300
BsrFI RCCGGY 1 cut(s) 138
BsrI ACTGG 1 cut(s) 79
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 2 cut(s) 349, 420
BssMI GATC 4 cut(s) 315, 439, 570, 592
BssT1I CCWWGG 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 2 cut(s) 169, 457
Bst6I CTCTTC 2 cut(s) 98, 190
BstC8I GCNNGC 4 cut(s) 22, 47, 140, 401
BstDEI CTNAG 1 cut(s) 128
BstFNI CGCG 1 cut(s) 20
BstKTI GATC 4 cut(s) 318, 442, 573, 595
BstMAI GTCTC 1 cut(s) 300
BstMBI GATC 4 cut(s) 315, 439, 570, 592
BstMWI GCNNNNNNNGC 5 cut(s) 36, 42, 139, 144, 361
BstNI CCWGG 1 cut(s) 339
BstSCI CCNGG 2 cut(s) 337, 419
BstSLI GKGCMC 3 cut(s) 357, 506, 545
BstUI CGCG 1 cut(s) 20
BstV1I GCAGC 1 cut(s) 48
BstX2I RGATCY 2 cut(s) 439, 592
BstYI RGATCY 2 cut(s) 439, 592
BsuRI GGCC 3 cut(s) 133, 142, 428
BtsI GCAGTG 1 cut(s) 37
BtsIMutI CAGTG 1 cut(s) 37
BveI ACCTGC 1 cut(s) 214
Cac8I GCNNGC 4 cut(s) 22, 47, 140, 401
CaiI CAGNNNCTG 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 1 cut(s) 132
Csp6I GTAC 3 cut(s) 271, 301, 346
CviAII CATG 2 cut(s) 163, 283
CviJI RGCY 9 cut(s) 39, 49, 133, 142, 147, 364, 428, 475, 586
CviKI_1 RGCY 9 cut(s) 39, 49, 133, 142, 147, 364, 428, 475, 586
CviQI GTAC 3 cut(s) 271, 301, 346
DdeI CTNAG 1 cut(s) 128
DpnI GATC 4 cut(s) 317, 441, 572, 594
DpnII GATC 4 cut(s) 315, 439, 570, 592
EaeI YGGCCR 1 cut(s) 140
Eam1104I CTCTTC 2 cut(s) 98, 190
EarI CTCTTC 2 cut(s) 98, 190
Eco130I CCWWGG 1 cut(s) 349
Eco147I AGGCCT 1 cut(s) 428
Eco31I GGTCTC 1 cut(s) 300
Eco32I GATATC 1 cut(s) 408
EcoO109I RGGNCCY 1 cut(s) 132
EcoRII CCWGG 1 cut(s) 337
EcoRV GATATC 1 cut(s) 408
EcoT14I CCWWGG 1 cut(s) 349
ErhI CCWWGG 1 cut(s) 349
FaeI CATG 2 cut(s) 166, 286
FaiI YATR 5 cut(s) 164, 266, 284, 569, 619
FaqI GGGAC 1 cut(s) 466
FatI CATG 2 cut(s) 162, 282
FbaI TGATCA 1 cut(s) 570
Fnu4HI GCNGC 4 cut(s) 37, 40, 43, 380
Fsp4HI GCNGC 4 cut(s) 37, 40, 43, 380
FspBI CTAG 1 cut(s) 255
GluI GCNGC 4 cut(s) 37, 40, 43, 380
GsaI CCCAGC 1 cut(s) 453
HaeIII GGCC 3 cut(s) 133, 142, 428
HapII CCGG 3 cut(s) 139, 279, 420
Hin1II CATG 2 cut(s) 166, 286
HindIII AAGCTT 2 cut(s) 145, 473
HpaII CCGG 3 cut(s) 139, 279, 420
HphI GGTGA 6 cut(s) 7, 202, 238, 469, 499, 575
Hpy166II GTNNAC 4 cut(s) 118, 172, 301, 543
Hpy188I TCNGA 1 cut(s) 444
Hpy8I GTNNAC 4 cut(s) 118, 172, 301, 543
Hpy99I CGWCG 1 cut(s) 592
HpyAV CCTTC 3 cut(s) 211, 478, 490
HpyCH4III ACNGT 2 cut(s) 169, 457
HpyCH4IV ACGT 3 cut(s) 320, 344, 534
HpyCH4V TGCA 4 cut(s) 223, 434, 543, 600
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 42, 139, 144, 361
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 3 cut(s) 320, 344, 534
Hsp92II CATG 2 cut(s) 166, 286
KroI GCCGGC 1 cut(s) 138
KroNI GCCGGC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 570
Kzo9I GATC 4 cut(s) 315, 439, 570, 592
Lsp1109I GCAGC 1 cut(s) 48
LweI GCATC 3 cut(s) 87, 210, 335
MaeI CTAG 1 cut(s) 255
MaeII ACGT 3 cut(s) 320, 344, 534
MaeIII GTNAC 1 cut(s) 226
MalI GATC 4 cut(s) 317, 441, 572, 594
MboI GATC 4 cut(s) 315, 439, 570, 592
MboII GAAGA 2 cut(s) 85, 177
MflI RGATCY 2 cut(s) 439, 592
MhlI GDGCHC 3 cut(s) 357, 506, 545
MluCI AATT 3 cut(s) 250, 520, 609
MnlI CCTC 5 cut(s) 71, 101, 131, 247, 418
MroNI GCCGGC 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 218
MspI CCGG 3 cut(s) 139, 279, 420
MspR9I CCNGG 2 cut(s) 339, 421
Mva1269I GAATGC 1 cut(s) 451
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 20
MwoI GCNNNNNNNGC 5 cut(s) 36, 42, 139, 144, 361
NaeI GCCGGC 1 cut(s) 140
NciI CCSGG 1 cut(s) 421
NdeII GATC 4 cut(s) 315, 439, 570, 592
NgoMIV GCCGGC 1 cut(s) 138
NlaIII CATG 2 cut(s) 166, 286
NlaIV GGNNCC 1 cut(s) 594
NmuCI GTSAC 1 cut(s) 226
PaqCI CACCTGC 1 cut(s) 214
PceI AGGCCT 1 cut(s) 428
PctI GAATGC 1 cut(s) 451
PdiI GCCGGC 1 cut(s) 140
PkrI GCNGC 4 cut(s) 38, 41, 44, 381
Psp6I CCWGG 1 cut(s) 337
PspFI CCCAGC 1 cut(s) 449
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 1 cut(s) 594
PspPI GGNCC 1 cut(s) 132
PstNI CAGNNNCTG 1 cut(s) 135
PsuI RGATCY 2 cut(s) 439, 592
RsaI GTAC 3 cut(s) 272, 302, 347
RsaNI GTAC 3 cut(s) 271, 301, 346
RseI CAYNNNNRTG 1 cut(s) 218
SatI GCNGC 4 cut(s) 37, 40, 43, 380
Sau3AI GATC 4 cut(s) 315, 439, 570, 592
Sau96I GGNCC 1 cut(s) 132
ScrFI CCNGG 2 cut(s) 339, 421
SduI GDGCHC 3 cut(s) 357, 506, 545
SfaNI GCATC 3 cut(s) 87, 210, 335
SfiI GGCCNNNNNGGCC 1 cut(s) 139
SmiMI CAYNNNNRTG 1 cut(s) 218
Sse9I AATT 3 cut(s) 250, 520, 609
SseBI AGGCCT 1 cut(s) 428
SsiI CCGC 5 cut(s) 18, 40, 43, 192, 380
SspMI CTAG 1 cut(s) 255
StuI AGGCCT 1 cut(s) 428
StyD4I CCNGG 2 cut(s) 337, 419
StyI CCWWGG 1 cut(s) 349
TaaI ACNGT 2 cut(s) 169, 457
TaiI ACGT 3 cut(s) 323, 347, 537
TasI AATT 3 cut(s) 250, 520, 609
TauI GCSGC 3 cut(s) 42, 45, 382
TscAI CASTG 1 cut(s) 37
TseFI GTSAC 1 cut(s) 226
TseI GCWGC 1 cut(s) 36
Tsp45I GTSAC 1 cut(s) 226
TspDTI ATGAA 1 cut(s) 139
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 1 cut(s) 37
VneI GTGCAC 1 cut(s) 541
XspI CTAG 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.