Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003613

Physical Location & Seq

Genomic Coordinates Forward (+)

43453873 .. 43462979

Gene Structure 9107 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1184300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2742 bp

ATGAAGAAAGTCTTTGATCAAACTGTTAGGGACATAAAGAGAGAGGTGAACAAGAAAGTGCTGAAAGTTCCTGGAATAGAACAGAAGGTTCTTGATGCTACAAGTAATGAGCCCTGGGGTCCTCATGGATCACTTCTTGCTGACATTGCACTGGCAACCCGAAACTATCATGAATACCAGATGATCATGTCCATCATTTGGAAGCGGCTAAGTGATACTGGAAAGAACTGGAGGCATGTTTACAAGGCTTTAATTATCTTGGAATACATGGTGGGTCATGGGTCAGAGCGTGTCATCGATGATATCAAGGAGCATGCGTATCAAATATCGACATTATCCGATTTTCAATACATTGATTCCAGTGGAAGGGACCAGGGAAGTAATGTCAGAAAGAAGTCTCAGAGTCTTGTGGCTCTTGTCAATGACAAAGAAAGGATAATTGAGGTTAGACAGAAAGCAGCTGCTAACAGGGACAAGTTTCGTAATACATCATCGACAGGTGGAATGTATAGGCCTGGTTCCCATTCAAGTCTAGGAGGATATGGTGACAAATATGACGATGATCATTACGAAGGCCGCTATGGAGGCAGGGATGAAGATAGGAATGGCTATGGGAGGGAACGAGAATGGGGCTATAGGGATGATGACCGGTACAGTCGTGATGGAGATCGTTATGGCAGAGAATATGATGAACGCAACGGGAGGGAAGGTTACAGGGATGATGATTATCGAGGAAGAAGTCGAAGTGTTGATGATTACCGTCATGGCTCTAGAAGTAGAAGCTCTGATAGAGAGAGGGAGCGTTCAATTGATGATGATGGTCATTATTCCTCTCGTGTTAGTGGTGCTAAAACTGATGACCAATCTCAAGATGGAAGGCTAAGCAGGAAATTTTCTGAACAAAACATTGGGGCTCCTTCTAGTTATGAGGAGGTTGTAAGTGAATCTCGAAGCCCTGTTCACAATGAAAGGGGTGGTGAAACTCCAGCAGCTTCTGCTCCTAGAGCTTCTTCTCCACCTTCAAGCAATAATCCAAGCCAAGCAACCAGTGTTCATGTTGCTTCTGCATCGCCTTCGAAGCAGGAAGTGGAGCCTTCAGATGAATTTGATCCACGTGGTTCAGTTTCAGGCGATGCATTTGGTTCAGTTTCAGCTGCCCAAGCTGCCCCAGCTGTCCAAGCAGCCCCGGTTACCTCAAACAATGTTGAGATGGACTTACTAGGTTCCCTTTCTGACTCATTCTCTTCAAATGCATTGGCCATTGTACCGACTACATCTTCTACTGCTACATTTGTAGCTGATCCCCTTGTAAACTCTAGTTCTGCAACCACATTTGCAGCAACTCCATCAACGTCTAATGTTATGAATCAGCCATTTGAAGATCCATTCGGTGATTCCCCTTTCAAAGCTCTACCTTCCAGTGAGGCGGTCCAACCTCAGCCACACACTTCCACGTCCACAGACTCTTTCACACCAACCATGAACCAGAGTGTAGATACAGCTTCCAACTTTCCCTTGGGGGATTCATTTTCTGCTGCAAGTTATTCTTTATCTGGTGTTTCCAGTGTTCAGACTCCAACAAACTCGCAATTTGTGTCTCAAGAGCTCTCTACTGAACACAACAACATGGATATTCTCGCTGATATTCTGCCACCTACCGGACCTTCACAGCAATCCTTTTCAAGTCCAACAGGTCAGCATCCACAGCCAAGTACTAATATGTATGGAAATTTTCATGTGCAGCCAGGATCTATAGTCCCTGATAATCAAACTGGATTTGCTGGACAACACAGTGGTGGGGGTTTTCCATCACAGGGAGGGCCTACAGCTACCATCACTTCACATGGAGCTCCTCAAACTCCAACAGGACCTACTGCACAGTTTAACAATGGAAGTTTCATTTCACAAGAAGGTGGATTTTCAGCTCCCTACAGCGGAAACTTCTTTTCACAACAGGGAGGTTACATGCCTCTTCATGCTCATAATGGACCGGCTGCACAGCTTAGTGGTGGGAACTTCCATCCACAACATGGTTCTGTCGGCTCGGTAGCTTCACAGGCCGCTCATCAAGCTCCATCTGGACGAGGTTCGCAACATAACAGTGATGTTCTGGGCAACGTTTTTTCACAAACAGGGCCAAACACCTCAATGGGTTCCCTGCAACCACTGTCATCTTCAACAGGGGCACTTGCTATAGTTGCTCAACCACCTAAAGACAGCAAGTTTGAAACTAAGTCAGCAGTTTGGGCTGATACGCTCAGCAGAGGCCTAGTTAATTTAAATATATCTGGAGCTAAAATTAACCCATTGAATGATATTGGAATTGATTTTGATTCCATTAATCGGAAGGAAAAGAGGATGGAGAAGCAGCCGGCAACTCCTGCAACATCTACTATTAACATGGGTAAAGCCATGGGATCTGGTTCTGGAATTGGCCGTGCAGGTGCAAGTGTTCTTAGGGCGCCACCAAACGCTATGGTGGGTTCTGGTATGGGCGTGGGGATGGGTCCTGGTCCGGGCATGGGTATGGGCCGCAGTCCTGGTGGAGGTATGGGCATGGGGGGGTATGGTGGCACAAATCAACCCATGGATATGGGGACGAACATGGGAATGGGAATGAATATGGGCTTGGGGATGCAAAGGCAAACCAGATTACCTCCTGGTTCAAACATGCCTAGTGGTTATAACCCCATGATGGGCGCAGGTGGTAATCCTCAGCAGCCATATGGCGGCTACCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0008289 GO:0012505 GO:0016020 GO:0030276 GO:0031090 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ENTH

Protein Analysis

914

Amino Acids

97.14

Weight (kDa)

6.05

Isoelectric Point (pI)

44.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ENTH	PF01417	26 - 146	2.3e-47	ENTH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0003613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43160 AT2G43160 AT2G43160 AT2G43160 AT2G43160 AT3G59290
fragaria_vesca	FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622
malus_domestica	MD00G1184300.v1.1 MD04G1031100.v1.1
prunus_persica	Prupe.1G182600_v2.0.a1 Prupe.1G182600_v2.0.a1
pyrus_communis	pycom13g29520
rosa_chinensis	RchiOBHm_Chr4g0408771
rosa_laevigata	RLG00000008632
rosa_multiflora	Rmu_co8298845.1_g000001 Rmu_sc0005878.1_g000006 Rmu_sc0007333.1_g000008
rosa_roxburghii	Rroxscaffold_5G00353120
rosa_rugosa	Rorug04G0087500 Rorug04G0087600 Rorug04G0087700
rosa_samantha	Rh4AG151800 Rh4BG149100 Rh4CG159900 Rh4DG145000
rosa_wichuraiana	Rw4G012450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2685
AarI	CACCTGC	2 cut(s)	2432, 2693
Acc36I	ACCTGC	2 cut(s)	2432, 2693
AccB1I	GGYRCC	1 cut(s)	2461
AccB7I	CCANNNNNTGG	2 cut(s)	198, 2030
AccBSI	CCGCTC	1 cut(s)	2063
AciI	CCGC	7 cut(s)	205, 577, 1427, 1935, 2061, 2533, 2730
AclI	AACGTT	1 cut(s)	2118
AclWI	GGATC	6 cut(s)	136, 1103, 1295, 1376, 1756, 2425
AcoI	YGGCCR	2 cut(s)	1257, 2434
AcsI	RAATTY	3 cut(s)	890, 1103, 1729
AcuI	CTGAAG	1 cut(s)	1080
AcvI	CACGTG	1 cut(s)	1115
AcyI	GRCGYC	1 cut(s)	2462
AfaI	GTAC	3 cut(s)	653, 1266, 1714
AgeI	ACCGGT	1 cut(s)	648
AjiI	CACGTC	1 cut(s)	1455
AjnI	CCWGG	8 cut(s)	70, 113, 372, 514, 1744, 2509, 2539, 2659
AjuI	GAANNNNNNNTTGG	4 cut(s)	891, 923, 2612, 2644
AleI	CACNNNNGTG	1 cut(s)	1794
Alw21I	GWGCWC	2 cut(s)	1608, 1852
Alw26I	GTCTC	2 cut(s)	402, 1602
AlwI	GGATC	6 cut(s)	136, 1103, 1295, 1376, 1756, 2425
AlwNI	CAGNNNCTG	1 cut(s)	995
AoxI	GGCC	9 cut(s)	512, 574, 1257, 1820, 2058, 2135, 2266, 2434, 2530
ApoI	RAATTY	3 cut(s)	890, 1103, 1729
ArsI	GACNNNNNNTTYG	2 cut(s)	2207, 2239
AseI	ATTAAT	1 cut(s)	2340
AsiGI	ACCGGT	1 cut(s)	648
AspLEI	GCGC	2 cut(s)	2464, 2702
AsuC2I	CCSGG	2 cut(s)	1187, 2517
AsuHPI	GGTGA	4 cut(s)	58, 557, 989, 1403
AsuII	TTCGAA	1 cut(s)	1076
AvaII	GGWCC	8 cut(s)	119, 370, 1429, 1661, 1868, 1988, 2507, 2513
BaeGI	GKGCMC	1 cut(s)	2188
BaeI	ACNNNNGTAYC	2 cut(s)	643, 676
BalI	TGGCCA	1 cut(s)	1259
BanI	GGYRCC	1 cut(s)	2461
BanII	GRGCYC	4 cut(s)	114, 916, 1608, 1852
BauI	CACGAG	1 cut(s)	834
BbrPI	CACGTG	1 cut(s)	1115
Bbv12I	GWGCWC	2 cut(s)	1608, 1852
BbvCI	CCTCAGC	2 cut(s)	1437, 2715
BceAI	ACGGC	1 cut(s)	2421
BcgI	CGANNNNNNTGC	2 cut(s)	1056, 1090
BciT130I	CCWGG	8 cut(s)	72, 115, 374, 516, 1746, 2511, 2541, 2661
BclI	TGATCA	3 cut(s)	16, 183, 562
BcnI	CCSGG	2 cut(s)	1187, 2517
BcoDI	GTCTC	2 cut(s)	402, 1602
BfaI	CTAG	8 cut(s)	533, 771, 921, 1002, 1220, 1317, 2270, 2676
BfmI	CTRYAG	5 cut(s)	634, 1752, 1824, 1930, 2193
BfoI	RGCGCY	1 cut(s)	2465
BfuAI	ACCTGC	2 cut(s)	2432, 2693
BlpI	GCTNAGC	2 cut(s)	881, 2258
BmcAI	AGTACT	1 cut(s)	1714
Bme18I	GGWCC	8 cut(s)	119, 370, 1429, 1661, 1868, 1988, 2507, 2513
BmgBI	CACGTC	1 cut(s)	1455
BmiI	GGNNCC	9 cut(s)	120, 371, 520, 915, 1092, 1225, 2155, 2463, 2508
BmsI	GCATC	5 cut(s)	85, 1076, 1123, 1708, 2625
BpmI	CTGGAG	3 cut(s)	250, 969, 2310
Bpu10I	CCTNAGC	2 cut(s)	1437, 2715
Bpu1102I	GCTNAGC	2 cut(s)	881, 2258
Bpu14I	TTCGAA	1 cut(s)	1076
BpuEI	CTTGAG	2 cut(s)	852, 1584
BpuMI	CCSGG	2 cut(s)	1187, 2517
Bsa29I	ATCGAT	1 cut(s)	297
BsaAI	YACGTR	1 cut(s)	1115
BsaBI	GATNNNNATC	2 cut(s)	666, 726
BsaHI	GRCGYC	1 cut(s)	2462
BsaJI	CCNNGG	7 cut(s)	113, 114, 373, 1185, 1515, 2412, 2586
BsaWI	WCCGGW	2 cut(s)	648, 1658
BsaXI	ACNNNNNCTCC	4 cut(s)	302, 332, 694, 724
Bse118I	RCCGGY	3 cut(s)	648, 1990, 2371
Bse1I	ACTGG	8 cut(s)	156, 223, 233, 360, 1047, 1419, 1563, 1777
Bse3DI	GCAATG	1 cut(s)	144
Bse8I	GATNNNNATC	2 cut(s)	666, 726
BseBI	CCWGG	8 cut(s)	72, 115, 374, 516, 1746, 2511, 2541, 2661
BseCI	ATCGAT	1 cut(s)	297
BseDI	CCNNGG	7 cut(s)	113, 114, 373, 1185, 1515, 2412, 2586
BseGI	GGATG	8 cut(s)	598, 646, 724, 1699, 2020, 2364, 2508, 2640
BseJI	GATNNNNATC	2 cut(s)	666, 726
BseMI	GCAATG	1 cut(s)	144
BseMII	CTCAG	4 cut(s)	413, 1451, 2272, 2729
BseNI	ACTGG	8 cut(s)	156, 223, 233, 360, 1047, 1419, 1563, 1777
BseRI	GAGGAG	2 cut(s)	944, 1842
BseSI	GKGCMC	1 cut(s)	2188
BseYI	CCCAGC	1 cut(s)	1168
BsgI	GTGCAG	4 cut(s)	1760, 1860, 1980, 2460
BshFI	GGCC	9 cut(s)	514, 576, 1259, 1822, 2060, 2137, 2268, 2436, 2532
BshNI	GGYRCC	1 cut(s)	2461
BshTI	ACCGGT	1 cut(s)	648
BshVI	ATCGAT	1 cut(s)	297
BsiHKAI	GWGCWC	2 cut(s)	1608, 1852
BsiSI	CCGG	6 cut(s)	649, 1187, 1659, 1991, 2372, 2516
BslFI	GGGAC	5 cut(s)	44, 383, 485, 1742, 2611
BsmAI	GTCTC	2 cut(s)	402, 1602
BsmFI	GGGAC	5 cut(s)	44, 383, 485, 1742, 2611
BsnI	GGCC	9 cut(s)	514, 576, 1259, 1822, 2060, 2137, 2268, 2436, 2532
Bsp119I	TTCGAA	1 cut(s)	1076
Bsp1286I	GDGCHC	5 cut(s)	114, 916, 1608, 1852, 2188
Bsp1720I	GCTNAGC	2 cut(s)	881, 2258
Bsp19I	CCATGG	2 cut(s)	2412, 2586
BspACI	CCGC	7 cut(s)	205, 577, 1427, 1935, 2061, 2533, 2730
BspANI	GGCC	9 cut(s)	514, 576, 1259, 1822, 2060, 2137, 2268, 2436, 2532
BspCNI	CTCAG	4 cut(s)	412, 1450, 2271, 2728
BspDI	ATCGAT	1 cut(s)	297
BspHI	TCATGA	1 cut(s)	169
BspLI	GGNNCC	9 cut(s)	120, 371, 520, 915, 1092, 1225, 2155, 2463, 2508
BspMI	ACCTGC	2 cut(s)	2432, 2693
BspPI	GGATC	6 cut(s)	136, 1103, 1295, 1376, 1756, 2425
BspT104I	TTCGAA	1 cut(s)	1076
BspT107I	GGYRCC	1 cut(s)	2461
BsrBI	CCGCTC	1 cut(s)	2063
BsrDI	GCAATG	1 cut(s)	144
BsrFI	RCCGGY	3 cut(s)	648, 1990, 2371
BsrI	ACTGG	8 cut(s)	156, 223, 233, 360, 1047, 1419, 1563, 1777
BssAI	RCCGGY	3 cut(s)	648, 1990, 2371
BssECI	CCNNGG	7 cut(s)	113, 114, 373, 1185, 1515, 2412, 2586
BssNI	GRCGYC	1 cut(s)	2462
BssSI	CACGAG	1 cut(s)	834
BssT1I	CCWWGG	3 cut(s)	1515, 2412, 2586
Bst2BI	CACGAG	1 cut(s)	834
Bst2UI	CCWGG	8 cut(s)	72, 115, 374, 516, 1746, 2511, 2541, 2661
Bst4CI	ACNGT	7 cut(s)	25, 656, 761, 1793, 1881, 2102, 2169
Bst6I	CTCTTC	2 cut(s)	1249, 1977
BstACI	GRCGYC	1 cut(s)	2462
BstAPI	GCANNNNNTGC	2 cut(s)	995, 2381
BstBAI	YACGTR	1 cut(s)	1115
BstBI	TTCGAA	1 cut(s)	1076
BstC8I	GCNNGC	2 cut(s)	315, 2373
BstDEI	CTNAG	9 cut(s)	209, 399, 881, 1437, 2003, 2232, 2258, 2456, 2715
BstDSI	CCRYGG	2 cut(s)	2412, 2586
BstEII	GGTNACC	1 cut(s)	1189
BstENI	CCTNNNNNAGG	1 cut(s)	2544
BstF5I	GGATG	8 cut(s)	598, 646, 724, 1699, 2020, 2364, 2508, 2640
BstH2I	RGCGCY	1 cut(s)	2465
BstHHI	GCGC	2 cut(s)	2464, 2702
BstMAI	GTCTC	2 cut(s)	402, 1602
BstNI	CCWGG	8 cut(s)	72, 115, 374, 516, 1746, 2511, 2541, 2661
BstNSI	RCATGY	4 cut(s)	239, 317, 1969, 2674
BstPI	GGTNACC	1 cut(s)	1189
BstSFI	CTRYAG	5 cut(s)	634, 1752, 1824, 1930, 2193
BstSLI	GKGCMC	1 cut(s)	2188
BstX2I	RGATCY	3 cut(s)	1381, 1748, 2417
BstXI	CCANNNNNNTGG	1 cut(s)	2593
BstYI	RGATCY	3 cut(s)	1381, 1748, 2417
Bsu15I	ATCGAT	1 cut(s)	297
BsuRI	GGCC	9 cut(s)	514, 576, 1259, 1822, 2060, 2137, 2268, 2436, 2532
BsuTUI	ATCGAT	1 cut(s)	297
BtgI	CCRYGG	2 cut(s)	2412, 2586
BtgZI	GCGATG	2 cut(s)	1053, 1146
BtrI	CACGTC	1 cut(s)	1455
BtsCI	GGATG	8 cut(s)	598, 646, 724, 1699, 2020, 2364, 2508, 2640
BtsIMutI	CAGTG	8 cut(s)	149, 367, 1054, 1426, 1570, 1798, 2107, 2165
BveI	ACCTGC	2 cut(s)	2432, 2693
Cac8I	GCNNGC	2 cut(s)	315, 2373
CaiI	CAGNNNCTG	1 cut(s)	995
CciI	TCATGA	1 cut(s)	169
CfoI	GCGC	2 cut(s)	2464, 2702
Cfr10I	RCCGGY	3 cut(s)	648, 1990, 2371
ClaI	ATCGAT	1 cut(s)	297
Csp6I	GTAC	3 cut(s)	652, 1265, 1713
CspAI	ACCGGT	1 cut(s)	648
CviQI	GTAC	3 cut(s)	652, 1265, 1713
DdeI	CTNAG	9 cut(s)	209, 399, 881, 1437, 2003, 2232, 2258, 2456, 2715
DinI	GGCGCC	1 cut(s)	2463
DraI	TTTAAA	1 cut(s)	2280
EaeI	YGGCCR	2 cut(s)	1257, 2434
Eam1104I	CTCTTC	2 cut(s)	1249, 1977
EarI	CTCTTC	2 cut(s)	1249, 1977
Ecl136II	GAGCTC	2 cut(s)	1606, 1850
Eco130I	CCWWGG	3 cut(s)	1515, 2412, 2586
Eco147I	AGGCCT	2 cut(s)	514, 2268
Eco24I	GRGCYC	4 cut(s)	114, 916, 1608, 1852
Eco32I	GATATC	1 cut(s)	304
Eco47I	GGWCC	8 cut(s)	119, 370, 1429, 1661, 1868, 1988, 2507, 2513
Eco53kI	GAGCTC	2 cut(s)	1606, 1850
Eco57I	CTGAAG	1 cut(s)	1080
Eco72I	CACGTG	1 cut(s)	1115
Eco91I	GGTNACC	1 cut(s)	1189
EcoICRI	GAGCTC	2 cut(s)	1606, 1850
EcoNI	CCTNNNNNAGG	1 cut(s)	2544
EcoO109I	RGGNCCY	4 cut(s)	119, 1820, 1868, 2507
EcoO65I	GGTNACC	1 cut(s)	1189
EcoRII	CCWGG	8 cut(s)	70, 113, 372, 514, 1744, 2509, 2539, 2659
EcoRV	GATATC	1 cut(s)	304
EcoT14I	CCWWGG	3 cut(s)	1515, 2412, 2586
EcoT22I	ATGCAT	2 cut(s)	1138, 1255
EcoT38I	GRGCYC	4 cut(s)	114, 916, 1608, 1852
EgeI	GGCGCC	1 cut(s)	2463
EheI	GGCGCC	1 cut(s)	2463
ErhI	CCWWGG	3 cut(s)	1515, 2412, 2586
FalI	AAGNNNNNCTT	3 cut(s)	28, 1531, 1563
FaqI	GGGAC	5 cut(s)	44, 383, 485, 1742, 2611
FauNDI	CATATG	1 cut(s)	2725
FbaI	TGATCA	3 cut(s)	16, 183, 562
FokI	GGATG	8 cut(s)	605, 653, 731, 1686, 2007, 2371, 2515, 2647
FriOI	GRGCYC	4 cut(s)	114, 916, 1608, 1852
FspBI	CTAG	8 cut(s)	533, 771, 921, 1002, 1220, 1317, 2270, 2676
GlaI	GCGC	2 cut(s)	2463, 2701
GsaI	CCCAGC	1 cut(s)	1172
GsuI	CTGGAG	3 cut(s)	250, 969, 2310
HaeII	RGCGCY	1 cut(s)	2465
HaeIII	GGCC	9 cut(s)	514, 576, 1259, 1822, 2060, 2137, 2268, 2436, 2532
HapII	CCGG	6 cut(s)	649, 1187, 1659, 1991, 2372, 2516
HhaI	GCGC	2 cut(s)	2464, 2702
Hin1I	GRCGYC	1 cut(s)	2462
Hin6I	GCGC	2 cut(s)	2462, 2700
HinP1I	GCGC	2 cut(s)	2462, 2700
HpaII	CCGG	6 cut(s)	649, 1187, 1659, 1991, 2372, 2516
HphI	GGTGA	4 cut(s)	58, 557, 989, 1403
Hpy166II	GTNNAC	5 cut(s)	49, 241, 961, 1312, 1458
Hpy8I	GTNNAC	5 cut(s)	49, 241, 961, 1312, 1458
HpyCH4III	ACNGT	7 cut(s)	25, 656, 761, 1793, 1881, 2102, 2169
HpyCH4IV	ACGT	4 cut(s)	1114, 1352, 1454, 2118
HpyF3I	CTNAG	9 cut(s)	209, 399, 881, 1437, 2003, 2232, 2258, 2456, 2715
HpySE526I	ACGT	4 cut(s)	1114, 1352, 1454, 2118
Hsp92I	GRCGYC	1 cut(s)	2462
HspAI	GCGC	2 cut(s)	2462, 2700
KasI	GGCGCC	1 cut(s)	2461
KroI	GCCGGC	1 cut(s)	2371
KroNI	GCCGGC	1 cut(s)	2373
Ksp22I	TGATCA	3 cut(s)	16, 183, 562
LweI	GCATC	5 cut(s)	85, 1076, 1123, 1708, 2625
MaeI	CTAG	8 cut(s)	533, 771, 921, 1002, 1220, 1317, 2270, 2676
MaeII	ACGT	4 cut(s)	1114, 1352, 1454, 2118
MaeIII	GTNAC	4 cut(s)	545, 710, 1189, 1961
MbiI	CCGCTC	1 cut(s)	2063
MboII	GAAGA	9 cut(s)	16, 608, 747, 1002, 1236, 1269, 1391, 1964, 2166
MfeI	CAATTG	1 cut(s)	807
MflI	RGATCY	3 cut(s)	1381, 1748, 2417
MhlI	GDGCHC	5 cut(s)	114, 916, 1608, 1852, 2188
MlsI	TGGCCA	1 cut(s)	1259
MluNI	TGGCCA	1 cut(s)	1259
Mly113I	GGCGCC	1 cut(s)	2462
MlyI	GAGTC	4 cut(s)	412, 1229, 1457, 1567
MmeI	TCCRAC	5 cut(s)	1456, 1530, 1601, 1712, 1886
Mox20I	TGGCCA	1 cut(s)	1259
Mph1103I	ATGCAT	2 cut(s)	1138, 1255
MroNI	GCCGGC	1 cut(s)	2371
MscI	TGGCCA	1 cut(s)	1259
MseI	TTAA	7 cut(s)	251, 1884, 2274, 2279, 2301, 2340, 2397
MslI	CAYNNNNRTG	6 cut(s)	1794, 2100, 2147, 2525, 2591, 2609
Msp20I	TGGCCA	1 cut(s)	1259
MspA1I	CMGCKG	4 cut(s)	461, 1154, 1172, 1935
MspI	CCGG	6 cut(s)	649, 1187, 1659, 1991, 2372, 2516
MunI	CAATTG	1 cut(s)	807
MvaI	CCWGG	8 cut(s)	72, 115, 374, 516, 1746, 2511, 2541, 2661
NaeI	GCCGGC	1 cut(s)	2373
NarI	GGCGCC	1 cut(s)	2462
NciI	CCSGG	2 cut(s)	1187, 2517
NcoI	CCATGG	2 cut(s)	2412, 2586
NdeI	CATATG	1 cut(s)	2725
NgoMIV	GCCGGC	1 cut(s)	2371
NlaIV	GGNNCC	9 cut(s)	120, 371, 520, 915, 1092, 1225, 2155, 2463, 2508
NmuCI	GTSAC	1 cut(s)	545
NsiI	ATGCAT	2 cut(s)	1138, 1255
NspI	RCATGY	4 cut(s)	239, 317, 1969, 2674
NspV	TTCGAA	1 cut(s)	1076
OliI	CACNNNNGTG	1 cut(s)	1794
PaeI	GCATGC	1 cut(s)	317
PagI	TCATGA	1 cut(s)	169
PaqCI	CACCTGC	2 cut(s)	2432, 2693
PasI	CCCWGGG	1 cut(s)	114
PceI	AGGCCT	2 cut(s)	514, 2268
PdiI	GCCGGC	1 cut(s)	2373
PfeI	GAWTC	6 cut(s)	356, 944, 1366, 1394, 1523, 2333
PflMI	CCANNNNNTGG	2 cut(s)	198, 2030
PfoI	TCCNGGA	1 cut(s)	70
PinAI	ACCGGT	1 cut(s)	648
PleI	GAGTC	4 cut(s)	411, 1229, 1457, 1567
PluTI	GGCGCC	1 cut(s)	2465
PmaCI	CACGTG	1 cut(s)	1115
PmlI	CACGTG	1 cut(s)	1115
PpsI	GAGTC	4 cut(s)	411, 1229, 1457, 1567
Ppu21I	YACGTR	1 cut(s)	1115
PpuMI	RGGWCCY	3 cut(s)	119, 1868, 2507
PshBI	ATTAAT	1 cut(s)	2340
PsiI	TTATAA	1 cut(s)	2685
Psp124BI	GAGCTC	2 cut(s)	1608, 1852
Psp1406I	AACGTT	1 cut(s)	2118
Psp5II	RGGWCCY	3 cut(s)	119, 1868, 2507
Psp6I	CCWGG	8 cut(s)	70, 113, 372, 514, 1744, 2509, 2539, 2659
PspCI	CACGTG	1 cut(s)	1115
PspEI	GGTNACC	1 cut(s)	1189
PspFI	CCCAGC	1 cut(s)	1168
PspGI	CCWGG	8 cut(s)	70, 113, 372, 514, 1744, 2509, 2539, 2659
PspN4I	GGNNCC	9 cut(s)	120, 371, 520, 915, 1092, 1225, 2155, 2463, 2508
PspPPI	RGGWCCY	3 cut(s)	119, 1868, 2507
PstNI	CAGNNNCTG	1 cut(s)	995
PsuI	RGATCY	3 cut(s)	1381, 1748, 2417
PvuII	CAGCTG	3 cut(s)	461, 1154, 1172
RsaI	GTAC	3 cut(s)	653, 1266, 1714
RsaNI	GTAC	3 cut(s)	652, 1265, 1713
RseI	CAYNNNNRTG	6 cut(s)	1794, 2100, 2147, 2525, 2591, 2609
SacI	GAGCTC	2 cut(s)	1608, 1852
SaqAI	TTAA	7 cut(s)	251, 1884, 2274, 2279, 2301, 2340, 2397
ScaI	AGTACT	1 cut(s)	1714
SchI	GAGTC	4 cut(s)	412, 1229, 1457, 1567
SduI	GDGCHC	5 cut(s)	114, 916, 1608, 1852, 2188
SfaNI	GCATC	5 cut(s)	85, 1076, 1123, 1708, 2625
SfcI	CTRYAG	5 cut(s)	634, 1752, 1824, 1930, 2193
SfoI	GGCGCC	1 cut(s)	2463
SfuI	TTCGAA	1 cut(s)	1076
SinI	GGWCC	8 cut(s)	119, 370, 1429, 1661, 1868, 1988, 2507, 2513
SmiI	ATTTAAAT	1 cut(s)	2280
SmiMI	CAYNNNNRTG	6 cut(s)	1794, 2100, 2147, 2525, 2591, 2609
SmlI	CTYRAG	2 cut(s)	867, 1599
SmoI	CTYRAG	2 cut(s)	867, 1599
SphI	GCATGC	1 cut(s)	317
SseBI	AGGCCT	2 cut(s)	514, 2268
SsiI	CCGC	7 cut(s)	205, 577, 1427, 1935, 2061, 2533, 2730
SspDI	GGCGCC	1 cut(s)	2461
SspMI	CTAG	8 cut(s)	533, 771, 921, 1002, 1220, 1317, 2270, 2676
SstI	GAGCTC	2 cut(s)	1608, 1852
StuI	AGGCCT	2 cut(s)	514, 2268
StyI	CCWWGG	3 cut(s)	1515, 2412, 2586
SwaI	ATTTAAAT	1 cut(s)	2280
TaaI	ACNGT	7 cut(s)	25, 656, 761, 1793, 1881, 2102, 2169
TaiI	ACGT	4 cut(s)	1117, 1355, 1457, 2121
TaqI	TCGA	7 cut(s)	297, 329, 494, 730, 742, 949, 1076
TatI	WGTACW	1 cut(s)	1712
TauI	GCSGC	5 cut(s)	208, 579, 2063, 2535, 2733
TfiI	GAWTC	6 cut(s)	356, 944, 1366, 1394, 1523, 2333
Tru1I	TTAA	7 cut(s)	251, 1884, 2274, 2279, 2301, 2340, 2397
Tru9I	TTAA	7 cut(s)	251, 1884, 2274, 2279, 2301, 2340, 2397
TscAI	CASTG	8 cut(s)	156, 367, 1054, 1426, 1570, 1798, 2107, 2172
TseFI	GTSAC	1 cut(s)	545
Tsp45I	GTSAC	1 cut(s)	545
TspRI	CASTG	8 cut(s)	156, 367, 1054, 1426, 1570, 1798, 2107, 2172
Van91I	CCANNNNNTGG	2 cut(s)	198, 2030
VpaK11BI	GGWCC	8 cut(s)	119, 370, 1429, 1661, 1868, 1988, 2507, 2513
VspI	ATTAAT	1 cut(s)	2340
XagI	CCTNNNNNAGG	1 cut(s)	2544
XapI	RAATTY	3 cut(s)	890, 1103, 1729
XbaI	TCTAGA	1 cut(s)	770
XceI	RCATGY	4 cut(s)	239, 317, 1969, 2674
XcmI	CCANNNNNNNNNTGG	3 cut(s)	869, 1513, 2027
XspI	CTAG	8 cut(s)	533, 771, 921, 1002, 1220, 1317, 2270, 2676
ZrmI	AGTACT	1 cut(s)	1714
Zsp2I	ATGCAT	2 cut(s)	1138, 1255

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.