Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0003613

Physical Location & Seq

Genomic Coordinates Reverse (-)

3470042 .. 3478129

Gene Structure 8088 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1031100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2802 bp

ATGAAGAAAGTCTTTGATCAAACTGTTAGGGACTTAAAGAGAGAGGTGAACAAGAAGGTCCTGAAAGTTCCTGGAATAGAACAGAAGGTTCTTGATGCTACTAGTAATGAGCCCTGGGGTCCTCATGGATCACTTCTTGCCGAAATTGCACAGGCAACCCGAAACTATCATGAATACCAAATGATAATGGCTGTCATTTGGAAGCGGTTAAGTGATACTGGAAAGAACTGGAGGCATGTTTACAAGGCTTTAATTGTCTTGGAATACATGGTGGCAAATGGGTCAGAACGTGTCATCGATGATATTAAGGAGCATGCTTATCAAATATCGACATTGTCCAGTTTTCAATACATTGATTCCAGTGGAAGGGACCAGGGAAGTAATGTCAGAAAGAAGTCTCAAAGTCTTGTGGCTCTTGTCAATGATAAAGAAAGGATAATTGAGGTTAGACAGAAAGCAGCTGCTAACAGGGACAAGTTCCGTAATACAGCATCAACAGGTGGAATGTATAGGCCCGGTTCCCATTCAAGTACAGGAGGATATGGTGACAAATATGACGATGATCGTTACGAAGGCCGCTACGGAGGCAGGGATGAAGATAGGAATGGCTATGGGAGGGAAAGAGAATGGGGCAATAGGGATGATGATCGGTACAGTCGTGACGGAGATCGTTATGGTAGAGAATATGATGAACGCAACGGGAGAGAAGGTTACAGGGATGATGATTACCGAGGAAGAAGTCAAAGTGTTGATGATTACCCTCATGGCTCTAGAAGTAGAAGCTCTGATAGAGAGAGGGAGCGTTCAGTTGATGATGATGGTCAATATTCCTCTCGTGGTAGTGGTGCTAAAGCTGATGACCAATCCCAGGATGGAAGGCTAAGTAGGAAATTTTCTGAACAAAACATTGGGGCTCCTCCTAGTTATGAGGAGGTTGTAAGTGAATCTCGAAGCCCTGTTCACAGCGAAAGGGGTGGTGAAACTCCAACAGCTTCTGCTCCTAGGGCTTCATCTCCACCTTCAAACAATAATCCAAGCCAAGCAACCAGTATTCATGTTACTTCTGCATCTAATCCAAGCCAAGCAACCAGTGTTCATGTTGCTTCTGCATCACCTGCAAAACAGGAAGTGGAGCCTTCAGATGAATTTGATCCACGTGGTTCTGTTTCAGCTACCCCAGCTCCCCAAGCTGCCCCAGCTGCCCCAGCTGTCCAAGCAGCCCCGGTTATCTCAAACAATGTTGAGATGGACTTACTAGGTTCCCTGTCAGACTCATTCTCTTCAAATGCATTGGCCATTGTACCGACTACACCTTCTACTGCTACATTTGAAGTTGATCCCCATGCGAACTCTAGTTCTACAACCACATTTACGGCAACTCCGCCAGCATCTAATGTTATGAATCAGTCATTTGAAGATCCATTCGGTGACTCCCCCTTCAAAGCTCTCCCTTCCAGTGAGACGGTCCAACCTCAAACACACACTTCCACGTCCACAGACTCTTTCCCACCAACTGTGAACCAGAGTGCAGATACAGCTTCCAACTTTCCCTTTGGGGATTCATTTTCTGCTGCAAATTATTCGTCATCTGGTGTTTCCAGTGTTCAAACTCCAACAAACTCACAATTTACGTCTCAAGAACAGTCTACTGAAAACATGGATATTCTTGCTGATATTTTGCCACCTACTGGACCTTCACAGCAGTCCTTTTCAGGTCCAACAGGTCAACATCCACAGCCAAGTGCTAATATGTATGGGAATTTTCATGTGCAGCCAGGATCTGTAGTCCCTCATAATCAAACTGGATTTGCTGGACAATACAGCAGTGGGGGTTTTCCGTCACAGGGAGGGCCTACAGCTCCCATCACTTCACATGGGGCTCCTCAAACTCCAACAGGGCCTACTGCACAGTTTAACAATGGAAATTTCATTCCACAACAAGGTGGGTTTTCAGCTCCCAACAGCGGAAACTATTTTTCACAACAGGGAGGTTACATGGCTCCTCATGCTCATAATGGACCGGCTGCACAACTTAATGGTGGGAACTTCCATCCACAAGGGAACTTCCATCCTCAAGGTTCTGTCGGCCCAGTAGCTTCACAGGCCACTACTCAAATTCCACCTGGACCAGGTTTGCAACATAATAGTGATGTTCTGGGCAACCTTTTATCACAAAAAGGACCAAACACCTCAATGGGTTCCCAGCAACCGCTGTCATCATCAACAGGGGCACTTGCTATAGTTGCTCAACCACCTAAAGACAGCAAGTTTGAACCGAAGTCAGCAGTTTGGGCTGATACACTCAGCAGAGGCCTAGTTAATTTAAATATATCTGGAGCTAAAATTAATCCATTGAATGATATTGGAATTGATTTTGATTCCATTAATCGGAAGGAAAAGAGGATGGAGAAGCAGCCGGCAACTCCTGCAACATCTACTATTAACATGGGTAAAGCCATGGGATCTGGTTCTGGAATTGGCCGTGCAGGTGCAAGTGTTCTTAGGGCTCCACCAAACGCGATGATGGGTTCAGGTATGGGCGGGGGGATGGGTCCGGGCATGGGAATGGCTATGGGTATGGGTGGGGGTCCAGGTGGAGGTATGGGCATGGGTGGCTACGGTGGCACGAATCAACCCATGGGTATGGGGATGGGGATGAACATGGGAATGGGAATGAATATGGGCATGGGGATGCAAGCGCAAACCGGATTACCTCCTGGTTCAAACATGCCTCCCGGCTATAACCCCATGATGGGCGCAGGTGGTTATCCTCAGCAGCCATATGGCGGTGGCTACCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0005794 GO:0005886 GO:0008289 GO:0012505 GO:0016020 GO:0030276 GO:0031090 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0071944 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

ENTH

Protein Analysis

934

Amino Acids

98.73

Weight (kDa)

5.95

Isoelectric Point (pI)

44.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ENTH	PF01417	26 - 146	4.4e-49	ENTH domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003613)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G43160 AT2G43160 AT2G43160 AT2G43160 AT2G43160 AT3G59290
fragaria_vesca	FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622 FvH4_4g11622
malus_domestica	MD00G1184300.v1.1 MD04G1031100.v1.1
prunus_persica	Prupe.1G182600_v2.0.a1 Prupe.1G182600_v2.0.a1
pyrus_communis	pycom13g29520
rosa_chinensis	RchiOBHm_Chr4g0408771
rosa_laevigata	RLG00000008632
rosa_multiflora	Rmu_co8298845.1_g000001 Rmu_sc0005878.1_g000006 Rmu_sc0007333.1_g000008
rosa_roxburghii	Rroxscaffold_5G00353120
rosa_rugosa	Rorug04G0087500 Rorug04G0087600 Rorug04G0087700
rosa_samantha	Rh4AG151800 Rh4BG149100 Rh4CG159900 Rh4DG145000
rosa_wichuraiana	Rw4G012450

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	3 cut(s)	1123, 2477, 2750
Acc36I	ACCTGC	3 cut(s)	1123, 2477, 2750
AccB7I	CCANNNNNTGG	1 cut(s)	1688
AccI	GTMKAC	1 cut(s)	1646
AccII	CGCG	1 cut(s)	2519
AciI	CCGC	7 cut(s)	205, 577, 1382, 1965, 2210, 2541, 2787
AclWI	GGATC	6 cut(s)	136, 1145, 1331, 1412, 1786, 2470
AcoI	YGGCCR	2 cut(s)	1293, 2479
AcsI	RAATTY	5 cut(s)	890, 1145, 1759, 1924, 2115
AcuI	CTGAAG	1 cut(s)	1122
AcvI	CACGTG	1 cut(s)	1157
AfaI	GTAC	3 cut(s)	532, 653, 1302
AflIII	ACRYGT	1 cut(s)	289
AhlI	ACTAGT	1 cut(s)	101
AjiI	CACGTC	1 cut(s)	1491
AjnI	CCWGG	9 cut(s)	70, 113, 372, 867, 1774, 2122, 2128, 2590, 2716
AjuI	GAANNNNNNNTTGG	2 cut(s)	891, 923
Alw26I	GTCTC	3 cut(s)	402, 1454, 1638
AlwI	GGATC	6 cut(s)	136, 1145, 1331, 1412, 1786, 2470
AlwNI	CAGNNNCTG	2 cut(s)	995, 1781
AoxI	GGCC	9 cut(s)	512, 574, 1293, 1850, 1898, 2086, 2103, 2311, 2479
ApoI	RAATTY	5 cut(s)	890, 1145, 1759, 1924, 2115
ArsI	GACNNNNNNTTYG	4 cut(s)	1568, 1600, 2252, 2284
AseI	ATTAAT	2 cut(s)	2346, 2385
AspA2I	CCTAGG	1 cut(s)	1001
AspLEI	GCGC	2 cut(s)	2701, 2759
AsuC2I	CCSGG	4 cut(s)	516, 1223, 2556, 2736
AsuHPI	GGTGA	5 cut(s)	58, 557, 989, 1104, 1439
AvrII	CCTAGG	1 cut(s)	1001
BaeGI	GKGCMC	1 cut(s)	2233
BaeI	ACNNNNGTAYC	2 cut(s)	643, 676
BalI	TGGCCA	1 cut(s)	1295
BanII	GRGCYC	4 cut(s)	114, 916, 1882, 2509
BauI	CACGAG	1 cut(s)	834
BbrPI	CACGTG	1 cut(s)	1157
BbvCI	CCTCAGC	1 cut(s)	2772
BceAI	ACGGC	2 cut(s)	1389, 2466
BcgI	CGANNNNNNTGC	2 cut(s)	1563, 1597
BciT130I	CCWGG	9 cut(s)	72, 115, 374, 869, 1776, 2124, 2130, 2592, 2718
BclI	TGATCA	1 cut(s)	16
BcnI	CCSGG	4 cut(s)	516, 1223, 2556, 2736
BcoDI	GTCTC	3 cut(s)	402, 1454, 1638
BcuI	ACTAGT	1 cut(s)	101
BfaI	CTAG	7 cut(s)	102, 771, 921, 1002, 1256, 1353, 2315
BfmI	CTRYAG	3 cut(s)	1782, 1854, 2238
BfuAI	ACCTGC	3 cut(s)	1123, 2477, 2750
BlnI	CCTAGG	1 cut(s)	1001
BmgBI	CACGTC	1 cut(s)	1491
BmrI	ACTGGG	1 cut(s)	2084
BmsI	GCATC	6 cut(s)	85, 500, 1076, 1118, 1397, 2682
BmuI	ACTGGG	1 cut(s)	2084
BpmI	CTGGAG	2 cut(s)	250, 2355
Bpu10I	CCTNAGC	1 cut(s)	2772
BpuEI	CTTGAG	2 cut(s)	1620, 2058
BpuMI	CCSGG	4 cut(s)	516, 1223, 2556, 2736
Bsa29I	ATCGAT	1 cut(s)	297
BsaAI	YACGTR	1 cut(s)	1157
BsaBI	GATNNNNATC	1 cut(s)	645
BsaJI	CCNNGG	9 cut(s)	113, 114, 373, 730, 867, 1001, 1221, 2457, 2637
BsaWI	WCCGGW	1 cut(s)	2705
BsaXI	ACNNNNNCTCC	6 cut(s)	694, 724, 1165, 1195, 1363, 1393
Bse118I	RCCGGY	2 cut(s)	2020, 2416
Bse8I	GATNNNNATC	1 cut(s)	645
BseBI	CCWGG	9 cut(s)	72, 115, 374, 869, 1776, 2124, 2130, 2592, 2718
BseCI	ATCGAT	1 cut(s)	297
BseDI	CCNNGG	9 cut(s)	113, 114, 373, 730, 867, 1001, 1221, 2457, 2637
BseJI	GATNNNNATC	1 cut(s)	645
BseMII	CTCAG	2 cut(s)	2317, 2786
BseRI	GAGGAG	4 cut(s)	906, 944, 1872, 1992
BseSI	GKGCMC	1 cut(s)	2233
BseYI	CCCAGC	4 cut(s)	1177, 1195, 1204, 2202
BsgI	GTGCAG	5 cut(s)	1548, 1790, 1890, 2010, 2505
Bsh1236I	CGCG	1 cut(s)	2519
BshFI	GGCC	9 cut(s)	514, 576, 1295, 1852, 1900, 2088, 2105, 2313, 2481
BshVI	ATCGAT	1 cut(s)	297
BsiSI	CCGG	7 cut(s)	516, 1223, 2021, 2417, 2555, 2706, 2736
BslFI	GGGAC	4 cut(s)	44, 383, 485, 1772
BsmAI	GTCTC	3 cut(s)	402, 1454, 1638
BsmBI	CGTCTC	2 cut(s)	1454, 1638
BsmFI	GGGAC	4 cut(s)	44, 383, 485, 1772
BsnI	GGCC	9 cut(s)	514, 576, 1295, 1852, 1900, 2088, 2105, 2313, 2481
Bsp1286I	GDGCHC	5 cut(s)	114, 916, 1882, 2233, 2509
Bsp19I	CCATGG	2 cut(s)	2457, 2637
BspACI	CCGC	7 cut(s)	205, 577, 1382, 1965, 2210, 2541, 2787
BspANI	GGCC	9 cut(s)	514, 576, 1295, 1852, 1900, 2088, 2105, 2313, 2481
BspCNI	CTCAG	2 cut(s)	2316, 2785
BspDI	ATCGAT	1 cut(s)	297
BspFNI	CGCG	1 cut(s)	2519
BspHI	TCATGA	1 cut(s)	169
BspMI	ACCTGC	3 cut(s)	1123, 2477, 2750
BspPI	GGATC	6 cut(s)	136, 1145, 1331, 1412, 1786, 2470
BsrFI	RCCGGY	2 cut(s)	2020, 2416
BssAI	RCCGGY	2 cut(s)	2020, 2416
BssECI	CCNNGG	9 cut(s)	113, 114, 373, 730, 867, 1001, 1221, 2457, 2637
BssSI	CACGAG	1 cut(s)	834
BssT1I	CCWWGG	3 cut(s)	1001, 2457, 2637
Bst2BI	CACGAG	1 cut(s)	834
Bst2UI	CCWGG	9 cut(s)	72, 115, 374, 869, 1776, 2124, 2130, 2592, 2718
Bst4CI	ACNGT	7 cut(s)	25, 656, 1465, 1516, 1644, 1911, 2621
Bst6I	CTCTTC	1 cut(s)	1285
BstAPI	GCANNNNNTGC	2 cut(s)	1115, 2426
BstBAI	YACGTR	1 cut(s)	1157
BstC8I	GCNNGC	4 cut(s)	315, 1386, 2418, 2697
BstDEI	CTNAG	4 cut(s)	881, 2303, 2501, 2772
BstDSI	CCRYGG	2 cut(s)	2457, 2637
BstENI	CCTNNNNNAGG	1 cut(s)	2127
BstFNI	CGCG	1 cut(s)	2519
BstHHI	GCGC	2 cut(s)	2701, 2759
BstMAI	GTCTC	3 cut(s)	402, 1454, 1638
BstNI	CCWGG	9 cut(s)	72, 115, 374, 869, 1776, 2124, 2130, 2592, 2718
BstNSI	RCATGY	3 cut(s)	239, 317, 2731
BstSFI	CTRYAG	3 cut(s)	1782, 1854, 2238
BstSLI	GKGCMC	1 cut(s)	2233
BstUI	CGCG	1 cut(s)	2519
BstX2I	RGATCY	3 cut(s)	1417, 1778, 2462
BstXI	CCANNNNNNTGG	1 cut(s)	2644
BstYI	RGATCY	3 cut(s)	1417, 1778, 2462
Bsu15I	ATCGAT	1 cut(s)	297
BsuRI	GGCC	9 cut(s)	514, 576, 1295, 1852, 1900, 2088, 2105, 2313, 2481
BsuTUI	ATCGAT	1 cut(s)	297
BtgI	CCRYGG	2 cut(s)	2457, 2637
BtgZI	GCGATG	1 cut(s)	2534
BtrI	CACGTC	1 cut(s)	1491
BtsI	GCAGTG	1 cut(s)	1831
BtsIMutI	CAGTG	5 cut(s)	367, 1096, 1462, 1606, 1831
BveI	ACCTGC	3 cut(s)	1123, 2477, 2750
Cac8I	GCNNGC	4 cut(s)	315, 1386, 2418, 2697
CaiI	CAGNNNCTG	2 cut(s)	995, 1781
CciI	TCATGA	1 cut(s)	169
CfoI	GCGC	2 cut(s)	2701, 2759
Cfr10I	RCCGGY	2 cut(s)	2020, 2416
ClaI	ATCGAT	1 cut(s)	297
CsiI	ACCWGGT	1 cut(s)	2128
Csp6I	GTAC	3 cut(s)	531, 652, 1301
CviQI	GTAC	3 cut(s)	531, 652, 1301
DdeI	CTNAG	4 cut(s)	881, 2303, 2501, 2772
DraI	TTTAAA	1 cut(s)	2325
EaeI	YGGCCR	2 cut(s)	1293, 2479
Eam1104I	CTCTTC	1 cut(s)	1285
EarI	CTCTTC	1 cut(s)	1285
EciI	GGCGGA	1 cut(s)	1371
Eco130I	CCWWGG	3 cut(s)	1001, 2457, 2637
Eco147I	AGGCCT	1 cut(s)	2313
Eco24I	GRGCYC	4 cut(s)	114, 916, 1882, 2509
Eco57I	CTGAAG	1 cut(s)	1122
Eco72I	CACGTG	1 cut(s)	1157
EcoNI	CCTNNNNNAGG	1 cut(s)	2127
EcoO109I	RGGNCCY	4 cut(s)	58, 119, 1850, 1898
EcoRII	CCWGG	9 cut(s)	70, 113, 372, 867, 1774, 2122, 2128, 2590, 2716
EcoT14I	CCWWGG	3 cut(s)	1001, 2457, 2637
EcoT22I	ATGCAT	1 cut(s)	1291
EcoT38I	GRGCYC	4 cut(s)	114, 916, 1882, 2509
ErhI	CCWWGG	3 cut(s)	1001, 2457, 2637
Esp3I	CGTCTC	2 cut(s)	1454, 1638
FalI	AAGNNNNNCTT	1 cut(s)	28
FaqI	GGGAC	4 cut(s)	44, 383, 485, 1772
FauI	CCCGC	1 cut(s)	2534
FauNDI	CATATG	1 cut(s)	2782
FbaI	TGATCA	1 cut(s)	16
FblI	GTMKAC	1 cut(s)	1646
FriOI	GRGCYC	4 cut(s)	114, 916, 1882, 2509
FspBI	CTAG	7 cut(s)	102, 771, 921, 1002, 1256, 1353, 2315
GlaI	GCGC	2 cut(s)	2700, 2758
GsaI	CCCAGC	4 cut(s)	1181, 1199, 1208, 2206
GsuI	CTGGAG	2 cut(s)	250, 2355
HaeIII	GGCC	9 cut(s)	514, 576, 1295, 1852, 1900, 2088, 2105, 2313, 2481
HapII	CCGG	7 cut(s)	516, 1223, 2021, 2417, 2555, 2706, 2736
HhaI	GCGC	2 cut(s)	2701, 2759
Hin6I	GCGC	2 cut(s)	2699, 2757
HinP1I	GCGC	2 cut(s)	2699, 2757
HincII	GTYRAC	1 cut(s)	1727
HindII	GTYRAC	1 cut(s)	1727
HinfI	GANTC	9 cut(s)	356, 944, 1271, 1402, 1430, 1499, 1559, 2378, 2629
HpaII	CCGG	7 cut(s)	516, 1223, 2021, 2417, 2555, 2706, 2736
HphI	GGTGA	5 cut(s)	58, 557, 989, 1104, 1439
Hpy166II	GTNNAC	7 cut(s)	49, 241, 961, 1494, 1519, 1647, 1727
Hpy188I	TCNGA	7 cut(s)	286, 389, 787, 898, 1141, 1270, 2391
Hpy188III	TCNNGA	9 cut(s)	61, 92, 170, 659, 771, 948, 1637, 2334, 2472
Hpy8I	GTNNAC	7 cut(s)	49, 241, 961, 1494, 1519, 1647, 1727
HpyCH4III	ACNGT	7 cut(s)	25, 656, 1465, 1516, 1644, 1911, 2621
HpyCH4IV	ACGT	4 cut(s)	289, 1156, 1490, 1631
HpyF3I	CTNAG	4 cut(s)	881, 2303, 2501, 2772
HpySE526I	ACGT	4 cut(s)	289, 1156, 1490, 1631
HspAI	GCGC	2 cut(s)	2699, 2757
KroI	GCCGGC	1 cut(s)	2416
KroNI	GCCGGC	1 cut(s)	2418
Ksp22I	TGATCA	1 cut(s)	16
LweI	GCATC	6 cut(s)	85, 500, 1076, 1118, 1397, 2682
MabI	ACCWGGT	1 cut(s)	2128
MaeI	CTAG	7 cut(s)	102, 771, 921, 1002, 1256, 1353, 2315
MaeII	ACGT	4 cut(s)	289, 1156, 1490, 1631
MaeIII	GTNAC	8 cut(s)	545, 566, 659, 710, 1057, 1427, 1839, 1991
MboII	GAAGA	5 cut(s)	16, 608, 747, 1272, 1427
MflI	RGATCY	3 cut(s)	1417, 1778, 2462
MhlI	GDGCHC	5 cut(s)	114, 916, 1882, 2233, 2509
MlsI	TGGCCA	1 cut(s)	1295
MluNI	TGGCCA	1 cut(s)	1295
MlyI	GAGTC	3 cut(s)	1265, 1424, 1493
MmeI	TCCRAC	6 cut(s)	1010, 1492, 1566, 1637, 1742, 1916
Mox20I	TGGCCA	1 cut(s)	1295
Mph1103I	ATGCAT	1 cut(s)	1291
MroNI	GCCGGC	1 cut(s)	2416
MscI	TGGCCA	1 cut(s)	1295
MslI	CAYNNNNRTG	6 cut(s)	2145, 2192, 2564, 2642, 2666, 2690
Msp20I	TGGCCA	1 cut(s)	1295
MspA1I	CMGCKG	5 cut(s)	461, 1199, 1208, 1965, 2212
MspI	CCGG	7 cut(s)	516, 1223, 2021, 2417, 2555, 2706, 2736
MvaI	CCWGG	9 cut(s)	72, 115, 374, 869, 1776, 2124, 2130, 2592, 2718
MvnI	CGCG	1 cut(s)	2519
NaeI	GCCGGC	1 cut(s)	2418
NciI	CCSGG	4 cut(s)	516, 1223, 2556, 2736
NcoI	CCATGG	2 cut(s)	2457, 2637
NdeI	CATATG	1 cut(s)	2782
NgoMIV	GCCGGC	1 cut(s)	2416
NmuCI	GTSAC	4 cut(s)	545, 659, 1427, 1839
NsiI	ATGCAT	1 cut(s)	1291
NspI	RCATGY	3 cut(s)	239, 317, 2731
PaeI	GCATGC	1 cut(s)	317
PagI	TCATGA	1 cut(s)	169
PaqCI	CACCTGC	3 cut(s)	1123, 2477, 2750
PasI	CCCWGGG	1 cut(s)	114
PceI	AGGCCT	1 cut(s)	2313
PcsI	WCGNNNNNNNCGW	1 cut(s)	655
PdiI	GCCGGC	1 cut(s)	2418
PfeI	GAWTC	6 cut(s)	356, 944, 1402, 1559, 2378, 2629
PflFI	GACNNNGTC	1 cut(s)	334
PflMI	CCANNNNNTGG	1 cut(s)	1688
PfoI	TCCNGGA	1 cut(s)	70
PleI	GAGTC	3 cut(s)	1265, 1424, 1493
PmaCI	CACGTG	1 cut(s)	1157
PmlI	CACGTG	1 cut(s)	1157
PpsI	GAGTC	3 cut(s)	1265, 1424, 1493
Ppu21I	YACGTR	1 cut(s)	1157
PpuMI	RGGWCCY	2 cut(s)	58, 119
PshBI	ATTAAT	2 cut(s)	2346, 2385
Psp5II	RGGWCCY	2 cut(s)	58, 119
Psp6I	CCWGG	9 cut(s)	70, 113, 372, 867, 1774, 2122, 2128, 2590, 2716
PspCI	CACGTG	1 cut(s)	1157
PspFI	CCCAGC	4 cut(s)	1177, 1195, 1204, 2202
PspGI	CCWGG	9 cut(s)	70, 113, 372, 867, 1774, 2122, 2128, 2590, 2716
PspPPI	RGGWCCY	2 cut(s)	58, 119
PstNI	CAGNNNCTG	2 cut(s)	995, 1781
PsuI	RGATCY	3 cut(s)	1417, 1778, 2462
PsyI	GACNNNGTC	1 cut(s)	334
PvuII	CAGCTG	3 cut(s)	461, 1199, 1208
RsaI	GTAC	3 cut(s)	532, 653, 1302
RsaNI	GTAC	3 cut(s)	531, 652, 1301
RseI	CAYNNNNRTG	6 cut(s)	2145, 2192, 2564, 2642, 2666, 2690
SchI	GAGTC	3 cut(s)	1265, 1424, 1493
SduI	GDGCHC	5 cut(s)	114, 916, 1882, 2233, 2509
SexAI	ACCWGGT	1 cut(s)	2128
SfaNI	GCATC	6 cut(s)	85, 500, 1076, 1118, 1397, 2682
SfcI	CTRYAG	3 cut(s)	1782, 1854, 2238
SmiI	ATTTAAAT	1 cut(s)	2325
SmiMI	CAYNNNNRTG	6 cut(s)	2145, 2192, 2564, 2642, 2666, 2690
SmlI	CTYRAG	2 cut(s)	1635, 2073
SmoI	CTYRAG	2 cut(s)	1635, 2073
SpeI	ACTAGT	1 cut(s)	101
SphI	GCATGC	1 cut(s)	317
SseBI	AGGCCT	1 cut(s)	2313
SsiI	CCGC	7 cut(s)	205, 577, 1382, 1965, 2210, 2541, 2787
SspI	AATATT	1 cut(s)	827
SspMI	CTAG	7 cut(s)	102, 771, 921, 1002, 1256, 1353, 2315
StuI	AGGCCT	1 cut(s)	2313
StyI	CCWWGG	3 cut(s)	1001, 2457, 2637
SwaI	ATTTAAAT	1 cut(s)	2325
TaaI	ACNGT	7 cut(s)	25, 656, 1465, 1516, 1644, 1911, 2621
TaiI	ACGT	4 cut(s)	292, 1159, 1493, 1634
TaqI	TCGA	3 cut(s)	297, 329, 949
TatI	WGTACW	1 cut(s)	530
TauI	GCSGC	1 cut(s)	579
TfiI	GAWTC	6 cut(s)	356, 944, 1402, 1559, 2378, 2629
TscAI	CASTG	5 cut(s)	367, 1096, 1462, 1606, 1831
TseFI	GTSAC	4 cut(s)	545, 659, 1427, 1839
Tsp45I	GTSAC	4 cut(s)	545, 659, 1427, 1839
TspGWI	ACGGA	4 cut(s)	470, 597, 678, 1827
TspRI	CASTG	5 cut(s)	367, 1096, 1462, 1606, 1831
Tth111I	GACNNNGTC	1 cut(s)	334
Van91I	CCANNNNNTGG	1 cut(s)	1688
VspI	ATTAAT	2 cut(s)	2346, 2385
XagI	CCTNNNNNAGG	1 cut(s)	2127
XapI	RAATTY	5 cut(s)	890, 1145, 1759, 1924, 2115
XbaI	TCTAGA	1 cut(s)	770
XceI	RCATGY	3 cut(s)	239, 317, 2731
XcmI	CCANNNNNNNNNTGG	1 cut(s)	869
XmaJI	CCTAGG	1 cut(s)	1001
XmiI	GTMKAC	1 cut(s)	1646
XspI	CTAG	7 cut(s)	102, 771, 921, 1002, 1256, 1353, 2315
Zsp2I	ATGCAT	1 cut(s)	1291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.