Rorug04G0087600

Clathrin interactor EPSIN

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003613
Physical Location & Seq
Forward (+)
13616455 .. 13618125
1671 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0087600.1

Sequence Viewer

Length: 579 bp
ATGGATAGAGCTTGGATCAAAATGGATAGAAGATCACCCGAACATCTATGCTCATCATTAAAGAAAGACAATAAGAAGGACTTGTATGGATTTGCGGATCCATCTTCCTTCTCTCATGATTCTGGAAAACGAGATGATAGATCCTATGCATTGTCAGTAAGATTAGAGAAAGGCAAGGAACAACAATTAATTTTTGCTCCTTACAATTTTGGGTTCCATTGGGTTTTGGCTGTAATTAATCCTTACAGTGATATGGTGTATTACTTGGACTCTCTCAATCCCGATGACTTAGACCATGGATTAAAGGGTGTTTTAGACGTCGCTCTAAGGATGTTTAATGCCCAAAAAGGAAGGAATCAGAAAAATGCTATTTGGAAAATCATACAGTGCCCTACTCAAGCTGGAAAAGTGGAATGCGGATATTATGTAATGAGGTTCATGAAAGCAATCATTGAAGATCCAACCATATTAACTAGAAGCAAGTTCAACAACCAAACTTACAATCAAGAAGAGATAGATGATGTTCGAGTAGAATGGGCTGACCTTGTTGAAACATTACTCCAAGATGATGTAGAATAG

Protein Analysis

192

Amino Acids

22.35

Weight (kDa)

5.62

Isoelectric Point (pI)

38.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C48 PF02902 60 - 186 1.7e-10 Ulp1 protease family, C-terminal catalytic domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 321
AciI CCGC 2 cut(s) 95, 417
AclWI GGATC 5 cut(s) 23, 92, 105, 135, 452
AcyI GRCGYC 1 cut(s) 318
AgsI TTSAA 3 cut(s) 455, 487, 551
AluBI AGCT 2 cut(s) 11, 401
AluI AGCT 2 cut(s) 11, 401
AlwI GGATC 5 cut(s) 23, 92, 105, 135, 452
AseI ATTAAT 2 cut(s) 188, 237
AsuHPI GGTGA 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 392
BamHI GGATCC 1 cut(s) 97
BccI CCATC 1 cut(s) 109
BfaI CTAG 1 cut(s) 474
BmiI GGNNCC 2 cut(s) 99, 215
BpuEI CTTGAG 1 cut(s) 381
BsaHI GRCGYC 1 cut(s) 318
BsaJI CCNNGG 1 cut(s) 295
BseDI CCNNGG 1 cut(s) 295
BseGI GGATG 1 cut(s) 336
BseSI GKGCMC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 419
Bsp1286I GDGCHC 1 cut(s) 392
Bsp143I GATC 5 cut(s) 15, 32, 97, 140, 457
Bsp19I CCATGG 1 cut(s) 295
BspACI CCGC 2 cut(s) 95, 417
BspHI TCATGA 2 cut(s) 115, 438
BspLI GGNNCC 2 cut(s) 99, 215
BspPI GGATC 5 cut(s) 23, 92, 105, 135, 452
BssECI CCNNGG 1 cut(s) 295
BssMI GATC 5 cut(s) 15, 32, 97, 140, 457
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 295
Bst4CI ACNGT 2 cut(s) 248, 387
Bst6I CTCTTC 1 cut(s) 504
BstACI GRCGYC 1 cut(s) 318
BstDEI CTNAG 2 cut(s) 289, 326
BstDSI CCRYGG 1 cut(s) 295
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 5 cut(s) 18, 35, 100, 143, 460
BstMBI GATC 5 cut(s) 15, 32, 97, 140, 457
BstSLI GKGCMC 1 cut(s) 392
BstX2I RGATCY 3 cut(s) 97, 140, 457
BstYI RGATCY 3 cut(s) 97, 140, 457
BtgI CCRYGG 1 cut(s) 295
BtsCI GGATG 1 cut(s) 336
BtsIMutI CAGTG 2 cut(s) 253, 392
CciI TCATGA 2 cut(s) 115, 438
CviAII CATG 3 cut(s) 116, 296, 439
CviJI RGCY 4 cut(s) 11, 230, 401, 539
CviKI_1 RGCY 4 cut(s) 11, 230, 401, 539
DdeI CTNAG 2 cut(s) 289, 326
DpnI GATC 5 cut(s) 17, 34, 99, 142, 459
DpnII GATC 5 cut(s) 15, 32, 97, 140, 457
Eam1104I CTCTTC 1 cut(s) 504
EarI CTCTTC 1 cut(s) 504
Eco130I CCWWGG 1 cut(s) 295
EcoT14I CCWWGG 1 cut(s) 295
EcoT22I ATGCAT 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 295
FaeI CATG 3 cut(s) 119, 299, 442
FalI AAGNNNNNCTT 2 cut(s) 65, 97
FatI CATG 3 cut(s) 115, 295, 438
FokI GGATG 1 cut(s) 343
FspBI CTAG 1 cut(s) 474
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 3 cut(s) 119, 299, 442
HinfI GANTC 3 cut(s) 119, 269, 355
HphI GGTGA 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 360
Hpy188III TCNNGA 5 cut(s) 116, 123, 281, 439, 506
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 3 cut(s) 70, 118, 345
HpyCH4III ACNGT 2 cut(s) 248, 387
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 1 cut(s) 149
HpyF3I CTNAG 2 cut(s) 289, 326
HpySE526I ACGT 1 cut(s) 318
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 3 cut(s) 119, 299, 442
Kzo9I GATC 5 cut(s) 15, 32, 97, 140, 457
LmnI GCTCC 1 cut(s) 202
LpnPI CCDG 2 cut(s) 108, 387
MaeI CTAG 1 cut(s) 474
MaeII ACGT 1 cut(s) 318
MalI GATC 5 cut(s) 17, 34, 99, 142, 459
MboI GATC 5 cut(s) 15, 32, 97, 140, 457
MboII GAAGA 4 cut(s) 42, 96, 467, 521
MflI RGATCY 3 cut(s) 97, 140, 457
MhlI GDGCHC 1 cut(s) 392
MluCI AATT 4 cut(s) 185, 189, 205, 234
MlyI GAGTC 1 cut(s) 263
MmeI TCCRAC 1 cut(s) 485
MnlI CCTC 1 cut(s) 426
Mph1103I ATGCAT 1 cut(s) 151
MseI TTAA 6 cut(s) 59, 188, 237, 302, 336, 470
Mva1269I GAATGC 1 cut(s) 419
NcoI CCATGG 1 cut(s) 295
NdeII GATC 5 cut(s) 15, 32, 97, 140, 457
NlaIII CATG 3 cut(s) 119, 299, 442
NlaIV GGNNCC 2 cut(s) 99, 215
NsiI ATGCAT 1 cut(s) 151
PagI TCATGA 2 cut(s) 115, 438
PctI GAATGC 1 cut(s) 419
PfeI GAWTC 2 cut(s) 119, 355
PleI GAGTC 1 cut(s) 263
PpsI GAGTC 1 cut(s) 263
PshBI ATTAAT 2 cut(s) 188, 237
PspN4I GGNNCC 2 cut(s) 99, 215
PsuI RGATCY 3 cut(s) 97, 140, 457
SaqAI TTAA 6 cut(s) 59, 188, 237, 302, 336, 470
Sau3AI GATC 5 cut(s) 15, 32, 97, 140, 457
SchI GAGTC 1 cut(s) 263
SduI GDGCHC 1 cut(s) 392
SetI ASST 5 cut(s) 13, 321, 403, 437, 546
SmlI CTYRAG 1 cut(s) 396
SmoI CTYRAG 1 cut(s) 396
Sse9I AATT 4 cut(s) 185, 189, 205, 234
SsiI CCGC 2 cut(s) 95, 417
SspMI CTAG 1 cut(s) 474
StyI CCWWGG 1 cut(s) 295
TaaI ACNGT 2 cut(s) 248, 387
TaiI ACGT 1 cut(s) 321
TaqI TCGA 1 cut(s) 526
TasI AATT 4 cut(s) 185, 189, 205, 234
TfiI GAWTC 2 cut(s) 119, 355
Tru1I TTAA 6 cut(s) 59, 188, 237, 302, 336, 470
Tru9I TTAA 6 cut(s) 59, 188, 237, 302, 336, 470
TscAI CASTG 2 cut(s) 253, 392
TspDTI ATGAA 2 cut(s) 427, 455
TspRI CASTG 2 cut(s) 253, 392
VspI ATTAAT 2 cut(s) 188, 237
XspI CTAG 1 cut(s) 474
ZraI GACGTC 1 cut(s) 319
Zsp2I ATGCAT 1 cut(s) 151
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.