Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012386

Physical Location & Seq

Genomic Coordinates Reverse (-)

45547932 .. 45550282

Gene Structure 2351 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1189600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 669 bp

ATGGAAAGCCCTAGACAGACATGCTTAAACCCTAATGCCATTTCACTCATTGATTGTATTGGAGGCCGAAACCAACACTCTCAGAGCTCCTCTTCCTCGTTCCTTAAGCACCCAAACATCATGCGACCTCCCAGAGGCGGCGGAAGTTTCAGAGGCTCCCGCGGTCGCAGTGGCGGTGGCGGTGGCCGCGGCTTTAATAGGGGTGGTGGTCGCGGTTTCTTCCGCGATGAGGGCCCACCTTCCGAAGTCGTGGAGGTTTCGACATTTGTTCATGCATGTGAGGGTGAGGCAGTGACAAAGCTCACAAATCCGAAGATACCCCATTTTAATGCCGCAATCTATCTGCAGAACAAGACTCAGATAGGGAAAGTTGATGAAATATTTGGTCCAATCAACGAATCTTACTTTTCGGTCAAAGTTATGGAAGGAATTGTGGCTACTTCGTATTCACAGGGTGATAAGTTCTATATTGACCCAATGAAGCTTCTTCCTCTTGAAAGATTTCTTCCGCAGCCAAAGGGACAGAAGCCAGCGTTTTCTCGAGGTGGAGGCCGTGGAAGGGGTGCTCCAAGGGGAAGGGGTTTTCCAAGGGGAGGGGGTTTTACGAGGGGAAGGGGTCCTCCGAGGGGTGGTAACCGTGGTGGCTTCAGGGGCAGAGGGAGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000454 GO:0001522 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0016074 GO:0022613 GO:0030515 GO:0031118 GO:0031429 GO:0031974 GO:0031976 GO:0031981 GO:0031984 GO:0032991 GO:0034357 GO:0034470 GO:0034513 GO:0034641 GO:0034660 GO:0042254 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055035 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Gar1

Protein Analysis

223

Amino Acids

23.87

Weight (kDa)

11.13

Isoelectric Point (pI)

37.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gar1	PF04410	83 - 169	1.9e-26	Gar1/Naf1 RNA binding region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0012386)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03920 AT5G18180
fragaria_vesca	FvH4_1g30320
malus_domestica	MD00G1189600.v1.1 MD05G1045500.v1.1
prunus_persica	Prupe.8G064800_v2.0.a1
pyrus_communis	pycom05g03790 pycom10g03750
rosa_chinensis	RchiOBHm_Chr3g0490211
rosa_laevigata	RLG00000022895
rosa_multiflora	Rmu_sc0018339.1_g000001 Rmu_sc0019534.1_g000007
rosa_roxburghii	Rroxscaffold_6G00393700
rosa_rugosa	Rorug03G0243000
rosa_samantha	Rh3AG291400 Rh3BG328400 Rh3CG325400 Rh3DG324700
rosa_wichuraiana	Rw3G025790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	4 cut(s)	162, 189, 213, 225
AcoI	YGGCCR	1 cut(s)	184
AcuI	CTGAAG	1 cut(s)	631
AdeI	CACNNNGTG	1 cut(s)	455
AfiI	CCNNNNNNNGG	7 cut(s)	134, 137, 229, 559, 593, 626, 629
AflII	CTTAAG	1 cut(s)	104
AgsI	TTSAA	1 cut(s)	497
AluBI	AGCT	3 cut(s)	87, 301, 484
AluI	AGCT	3 cut(s)	87, 301, 484
Alw21I	GWGCWC	2 cut(s)	89, 568
Ama87I	CYCGRG	1 cut(s)	540
AoxI	GGCC	4 cut(s)	64, 184, 232, 550
ApaI	GGGCCC	1 cut(s)	236
ApeKI	GCWGC	1 cut(s)	511
Asp700I	GAANNNNTTC	1 cut(s)	501
AspS9I	GGNCC	4 cut(s)	232, 233, 386, 617
AsuHPI	GGTGA	2 cut(s)	296, 467
AvaI	CYCGRG	1 cut(s)	540
AvaII	GGWCC	2 cut(s)	386, 617
BaeGI	GKGCMC	1 cut(s)	236
BanII	GRGCYC	2 cut(s)	89, 236
Bbv12I	GWGCWC	2 cut(s)	89, 568
BbvI	GCAGC	1 cut(s)	523
BceAI	ACGGC	1 cut(s)	537
BfaI	CTAG	1 cut(s)	12
BfmI	CTRYAG	1 cut(s)	344
BfrI	CTTAAG	1 cut(s)	104
BisI	GCNGC	5 cut(s)	139, 187, 190, 333, 512
BlsI	GCNGC	5 cut(s)	140, 188, 191, 334, 513
Bme18I	GGWCC	2 cut(s)	386, 617
BmeT110I	CYCGRG	1 cut(s)	540
BmgT120I	GGNCC	4 cut(s)	232, 233, 386, 617
BmiI	GGNNCC	3 cut(s)	157, 234, 618
BsaJI	CCNNGG	7 cut(s)	160, 187, 553, 569, 587, 623, 637
Bsc4I	CCNNNNNNNGG	7 cut(s)	134, 137, 229, 559, 593, 626, 629
BseDI	CCNNGG	7 cut(s)	160, 187, 553, 569, 587, 623, 637
BseLI	CCNNNNNNNGG	7 cut(s)	134, 137, 229, 559, 593, 626, 629
BseMII	CTCAG	2 cut(s)	95, 371
BseRI	GAGGAG	1 cut(s)	79
BseSI	GKGCMC	1 cut(s)	236
BseXI	GCAGC	1 cut(s)	523
Bsh1236I	CGCG	4 cut(s)	162, 189, 213, 225
Bsh1285I	CGRYCG	1 cut(s)	166
BshFI	GGCC	4 cut(s)	66, 186, 234, 552
BsiEI	CGRYCG	1 cut(s)	166
BsiHKAI	GWGCWC	2 cut(s)	89, 568
BsiHKCI	CYCGRG	1 cut(s)	540
BslFI	GGGAC	1 cut(s)	534
BslI	CCNNNNNNNGG	7 cut(s)	134, 137, 229, 559, 593, 626, 629
BsmFI	GGGAC	1 cut(s)	534
BsnI	GGCC	4 cut(s)	66, 186, 234, 552
BsoBI	CYCGRG	1 cut(s)	540
Bsp120I	GGGCCC	1 cut(s)	232
Bsp1286I	GDGCHC	3 cut(s)	89, 236, 568
BspANI	GGCC	4 cut(s)	66, 186, 234, 552
BspCNI	CTCAG	2 cut(s)	94, 370
BspFNI	CGCG	4 cut(s)	162, 189, 213, 225
BspLI	GGNNCC	3 cut(s)	157, 234, 618
BspMAI	CTGCAG	1 cut(s)	348
BspTI	CTTAAG	1 cut(s)	104
BssECI	CCNNGG	7 cut(s)	160, 187, 553, 569, 587, 623, 637
BssT1I	CCWWGG	2 cut(s)	569, 587
Bst4CI	ACNGT	1 cut(s)	638
Bst6I	CTCTTC	1 cut(s)	97
BstAFI	CTTAAG	1 cut(s)	104
BstC8I	GCNNGC	1 cut(s)	531
BstDEI	CTNAG	2 cut(s)	81, 357
BstDSI	CCRYGG	4 cut(s)	160, 187, 553, 637
BstEII	GGTNACC	1 cut(s)	632
BstENI	CCTNNNNNAGG	1 cut(s)	132
BstFNI	CGCG	4 cut(s)	162, 189, 213, 225
BstMCI	CGRYCG	1 cut(s)	166
BstMWI	GCNNNNNNNGC	3 cut(s)	186, 231, 651
BstNSI	RCATGY	2 cut(s)	24, 279
BstPI	GGTNACC	1 cut(s)	632
BstSFI	CTRYAG	1 cut(s)	344
BstSLI	GKGCMC	1 cut(s)	236
BstUI	CGCG	4 cut(s)	162, 189, 213, 225
BstV1I	GCAGC	1 cut(s)	523
BsuRI	GGCC	4 cut(s)	66, 186, 234, 552
BtgI	CCRYGG	4 cut(s)	160, 187, 553, 637
BtgZI	GCGATG	1 cut(s)	240
BtsI	GCAGTG	2 cut(s)	175, 297
BtsIMutI	CAGTG	2 cut(s)	175, 297
Cac8I	GCNNGC	1 cut(s)	531
Cfr13I	GGNCC	4 cut(s)	232, 233, 386, 617
Cfr42I	CCGCGG	2 cut(s)	163, 190
CviAII	CATG	4 cut(s)	21, 121, 272, 276
DdeI	CTNAG	2 cut(s)	81, 357
DraIII	CACNNNGTG	1 cut(s)	455
EaeI	YGGCCR	1 cut(s)	184
Eam1104I	CTCTTC	1 cut(s)	97
EarI	CTCTTC	1 cut(s)	97
EciI	GGCGGA	1 cut(s)	156
Ecl136II	GAGCTC	1 cut(s)	87
Eco130I	CCWWGG	2 cut(s)	569, 587
Eco24I	GRGCYC	2 cut(s)	89, 236
Eco47I	GGWCC	2 cut(s)	386, 617
Eco53kI	GAGCTC	1 cut(s)	87
Eco57I	CTGAAG	1 cut(s)	631
Eco88I	CYCGRG	1 cut(s)	540
Eco91I	GGTNACC	1 cut(s)	632
EcoICRI	GAGCTC	1 cut(s)	87
EcoNI	CCTNNNNNAGG	1 cut(s)	132
EcoO109I	RGGNCCY	2 cut(s)	232, 617
EcoO65I	GGTNACC	1 cut(s)	632
EcoT14I	CCWWGG	2 cut(s)	569, 587
EcoT22I	ATGCAT	1 cut(s)	277
EcoT38I	GRGCYC	2 cut(s)	89, 236
ErhI	CCWWGG	2 cut(s)	569, 587
FaeI	CATG	4 cut(s)	24, 124, 275, 279
FaiI	YATR	6 cut(s)	22, 122, 273, 277, 422, 468
FaqI	GGGAC	1 cut(s)	534
FatI	CATG	4 cut(s)	20, 120, 271, 275
FauI	CCCGC	1 cut(s)	167
Fnu4HI	GCNGC	5 cut(s)	139, 187, 190, 333, 512
FriOI	GRGCYC	2 cut(s)	89, 236
Fsp4HI	GCNGC	5 cut(s)	139, 187, 190, 333, 512
FspBI	CTAG	1 cut(s)	12
GluI	GCNGC	5 cut(s)	139, 187, 190, 333, 512
HaeIII	GGCC	4 cut(s)	66, 186, 234, 552
Hin1II	CATG	4 cut(s)	24, 124, 275, 279
HindIII	AAGCTT	1 cut(s)	482
HinfI	GANTC	2 cut(s)	355, 398
HphI	GGTGA	2 cut(s)	296, 467
Hpy188I	TCNGA	6 cut(s)	84, 152, 244, 312, 360, 624
Hpy188III	TCNNGA	2 cut(s)	494, 540
HpyAV	CCTTC	5 cut(s)	249, 419, 552, 570, 606
HpyCH4III	ACNGT	1 cut(s)	638
HpyCH4V	TGCA	2 cut(s)	275, 346
HpyF10VI	GCNNNNNNNGC	3 cut(s)	186, 231, 651
HpyF3I	CTNAG	2 cut(s)	81, 357
Hsp92II	CATG	4 cut(s)	24, 124, 275, 279
KspI	CCGCGG	2 cut(s)	163, 190
LmnI	GCTCC	3 cut(s)	92, 161, 571
LpnPI	CCDG	4 cut(s)	145, 437, 543, 634
Lsp1109I	GCAGC	1 cut(s)	523
MaeI	CTAG	1 cut(s)	12
MaeIII	GTNAC	2 cut(s)	292, 632
MboII	GAAGA	5 cut(s)	84, 211, 325, 479, 497
MhlI	GDGCHC	3 cut(s)	89, 236, 568
MluCI	AATT	1 cut(s)	429
MlyI	GAGTC	1 cut(s)	349
Mph1103I	ATGCAT	1 cut(s)	277
MroXI	GAANNNNTTC	1 cut(s)	501
MseI	TTAA	4 cut(s)	26, 105, 195, 327
MslI	CAYNNNNRTG	2 cut(s)	276, 327
MspA1I	CMGCKG	2 cut(s)	162, 189
MspCI	CTTAAG	1 cut(s)	104
MvnI	CGCG	4 cut(s)	162, 189, 213, 225
MwoI	GCNNNNNNNGC	3 cut(s)	186, 231, 651
NlaIII	CATG	4 cut(s)	24, 124, 275, 279
NlaIV	GGNNCC	3 cut(s)	157, 234, 618
NmuCI	GTSAC	1 cut(s)	292
NsiI	ATGCAT	1 cut(s)	277
NspI	RCATGY	2 cut(s)	24, 279
PaeR7I	CTCGAG	1 cut(s)	540
PdmI	GAANNNNTTC	1 cut(s)	501
PfeI	GAWTC	1 cut(s)	398
PkrI	GCNGC	5 cut(s)	140, 188, 191, 334, 513
PleI	GAGTC	1 cut(s)	349
PpsI	GAGTC	1 cut(s)	349
PpuMI	RGGWCCY	1 cut(s)	617
Psp124BI	GAGCTC	1 cut(s)	89
Psp5II	RGGWCCY	1 cut(s)	617
PspEI	GGTNACC	1 cut(s)	632
PspN4I	GGNNCC	3 cut(s)	157, 234, 618
PspOMI	GGGCCC	1 cut(s)	232
PspPI	GGNCC	4 cut(s)	232, 233, 386, 617
PspPPI	RGGWCCY	1 cut(s)	617
PstI	CTGCAG	1 cut(s)	348
RseI	CAYNNNNRTG	2 cut(s)	276, 327
SacI	GAGCTC	1 cut(s)	89
SacII	CCGCGG	2 cut(s)	163, 190
SaqAI	TTAA	4 cut(s)	26, 105, 195, 327
SatI	GCNGC	5 cut(s)	139, 187, 190, 333, 512
Sau96I	GGNCC	4 cut(s)	232, 233, 386, 617
SchI	GAGTC	1 cut(s)	349
SduI	GDGCHC	3 cut(s)	89, 236, 568
SetI	ASST	7 cut(s)	89, 130, 241, 258, 303, 486, 547
SfcI	CTRYAG	1 cut(s)	344
Sfr274I	CTCGAG	1 cut(s)	540
Sfr303I	CCGCGG	2 cut(s)	163, 190
SgrBI	CCGCGG	2 cut(s)	163, 190
SinI	GGWCC	2 cut(s)	386, 617
SlaI	CTCGAG	1 cut(s)	540
SmiMI	CAYNNNNRTG	2 cut(s)	276, 327
SmlI	CTYRAG	2 cut(s)	104, 540
SmoI	CTYRAG	2 cut(s)	104, 540
Sse9I	AATT	1 cut(s)	429
SspI	AATATT	1 cut(s)	381
SspMI	CTAG	1 cut(s)	12
SstI	GAGCTC	1 cut(s)	89
StyI	CCWWGG	2 cut(s)	569, 587
TaaI	ACNGT	1 cut(s)	638
TaqI	TCGA	2 cut(s)	260, 541
TaqII	GACCGA	1 cut(s)	400
TasI	AATT	1 cut(s)	429
TauI	GCSGC	4 cut(s)	141, 189, 192, 335
TfiI	GAWTC	1 cut(s)	398
Tru1I	TTAA	4 cut(s)	26, 105, 195, 327
Tru9I	TTAA	4 cut(s)	26, 105, 195, 327
TscAI	CASTG	2 cut(s)	175, 297
TseFI	GTSAC	1 cut(s)	292
TseI	GCWGC	1 cut(s)	511
Tsp45I	GTSAC	1 cut(s)	292
TspDTI	ATGAA	3 cut(s)	260, 390, 494
TspRI	CASTG	2 cut(s)	175, 297
Vha464I	CTTAAG	1 cut(s)	104
VpaK11BI	GGWCC	2 cut(s)	386, 617
XagI	CCTNNNNNAGG	1 cut(s)	132
XceI	RCATGY	2 cut(s)	24, 279
XhoI	CTCGAG	1 cut(s)	540
XmnI	GAANNNNTTC	1 cut(s)	501
XspI	CTAG	1 cut(s)	12
Zsp2I	ATGCAT	1 cut(s)	277

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.