Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0012386

Physical Location & Seq

Genomic Coordinates Reverse (-)

7778010 .. 7780840

Gene Structure 2831 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1045500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 540 bp

ATGCGACCTCCCAGAGGCGGCGGAGGGTTCAGAGGCTCCCGCGGTCGCAGTGGTGGTGGTCGCGGGTTTAACAGTGGCGGTCGCGGTTTCTTCCGCGATGAGGGCCCTCCTTCCGAAGTCGTAGAGGTTTCAACATTTGTTCATGCATGTGAGGGTGAGGCAGTCACGAAGCTCACGAATTCGAAGATACCCCATTTTAATGCCCCAATCTATCTGCAAAACAAGACTCAGATAGGGAAAGTTGATGAAATATTTGGTCCGATCAACGAATCTTATTTTTCGGTTAAAGTTATGGAAGGCATTGTGGCGACTTCGTATTCCCAGGGAGATAAGTTCTATATTGACCCAATGAAGCTTCTTCCTCTTGAAAGATTTCTTCCACAGCCAAAGGGACAGAAGCCAGCATTTTCTCGAGGCGGAGGCCGTGGAAGGGGTACTCCGAGGGGAAGAGGTTTTTCAAGGGGAAGGGGTCCTCCAAGGGGAAGGGGTCCTCCGAGGGGTGGTAACCGTGGTGGCTTCAGGGGCAGAGGGAGATTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000154 GO:0000454 GO:0001522 GO:0003674 GO:0003676 GO:0003723 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005732 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009987 GO:0010467 GO:0016020 GO:0016070 GO:0016072 GO:0016074 GO:0022613 GO:0030515 GO:0031118 GO:0031429 GO:0031974 GO:0031976 GO:0031981 GO:0031984 GO:0032991 GO:0034357 GO:0034470 GO:0034513 GO:0034641 GO:0034660 GO:0042254 GO:0042651 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044452 GO:0044464 GO:0046483 GO:0055035 GO:0070013 GO:0071704 GO:0071840 GO:0072588 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1902494 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

Gar1

Protein Analysis

180

Amino Acids

19.33

Weight (kDa)

11.39

Isoelectric Point (pI)

26.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Gar1	PF04410	40 - 126	1.7e-27	Gar1/Naf1 RNA binding region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Publication-ready

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Orthologous Genes (Group: OG0012386)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G03920 AT5G18180
fragaria_vesca	FvH4_1g30320
malus_domestica	MD00G1189600.v1.1 MD05G1045500.v1.1
prunus_persica	Prupe.8G064800_v2.0.a1
pyrus_communis	pycom05g03790 pycom10g03750
rosa_chinensis	RchiOBHm_Chr3g0490211
rosa_laevigata	RLG00000022895
rosa_multiflora	Rmu_sc0018339.1_g000001 Rmu_sc0019534.1_g000007
rosa_roxburghii	Rroxscaffold_6G00393700
rosa_rugosa	Rorug03G0243000
rosa_samantha	Rh3AG291400 Rh3BG328400 Rh3CG325400 Rh3DG324700
rosa_wichuraiana	Rw3G025790

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	4 cut(s)	42, 63, 84, 96
AciI	CCGC	9 cut(s)	18, 21, 40, 42, 63, 78, 84, 94, 417
AcsI	RAATTY	1 cut(s)	178
AcuI	CTGAAG	1 cut(s)	502
AfaI	GTAC	1 cut(s)	436
AfiI	CCNNNNNNNGG	7 cut(s)	14, 17, 100, 430, 479, 497, 500
AgsI	TTSAA	3 cut(s)	132, 368, 459
AjnI	CCWGG	1 cut(s)	321
AluBI	AGCT	2 cut(s)	172, 355
AluI	AGCT	2 cut(s)	172, 355
Ama87I	CYCGRG	1 cut(s)	411
AoxI	GGCC	2 cut(s)	103, 421
ApaI	GGGCCC	1 cut(s)	107
ApoI	RAATTY	1 cut(s)	178
Asp700I	GAANNNNTTC	1 cut(s)	372
AspS9I	GGNCC	5 cut(s)	103, 104, 257, 470, 488
AsuHPI	GGTGA	1 cut(s)	167
AsuII	TTCGAA	1 cut(s)	182
AvaI	CYCGRG	1 cut(s)	411
AvaII	GGWCC	3 cut(s)	257, 470, 488
BaeGI	GKGCMC	1 cut(s)	107
BanII	GRGCYC	1 cut(s)	107
BceAI	ACGGC	1 cut(s)	408
BciT130I	CCWGG	1 cut(s)	323
BisI	GCNGC	1 cut(s)	19
BlsI	GCNGC	1 cut(s)	20
Bme1390I	CCNGG	1 cut(s)	323
Bme18I	GGWCC	3 cut(s)	257, 470, 488
BmeT110I	CYCGRG	1 cut(s)	411
BmgT120I	GGNCC	5 cut(s)	103, 104, 257, 470, 488
BmiI	GGNNCC	4 cut(s)	37, 105, 471, 489
BmrFI	CCNGG	1 cut(s)	323
Bpu14I	TTCGAA	1 cut(s)	182
BsaJI	CCNNGG	8 cut(s)	40, 321, 322, 424, 440, 476, 494, 508
Bsc4I	CCNNNNNNNGG	7 cut(s)	14, 17, 100, 430, 479, 497, 500
BseBI	CCWGG	1 cut(s)	323
BseDI	CCNNGG	8 cut(s)	40, 321, 322, 424, 440, 476, 494, 508
BseLI	CCNNNNNNNGG	7 cut(s)	14, 17, 100, 430, 479, 497, 500
BseMII	CTCAG	1 cut(s)	242
BseSI	GKGCMC	1 cut(s)	107
Bsh1236I	CGCG	4 cut(s)	42, 63, 84, 96
Bsh1285I	CGRYCG	2 cut(s)	46, 82
BshFI	GGCC	2 cut(s)	105, 423
BsiEI	CGRYCG	2 cut(s)	46, 82
BsiHKCI	CYCGRG	1 cut(s)	411
BslFI	GGGAC	1 cut(s)	405
BslI	CCNNNNNNNGG	7 cut(s)	14, 17, 100, 430, 479, 497, 500
BsmFI	GGGAC	1 cut(s)	405
BsnI	GGCC	2 cut(s)	105, 423
BsoBI	CYCGRG	1 cut(s)	411
Bsp119I	TTCGAA	1 cut(s)	182
Bsp120I	GGGCCC	1 cut(s)	103
Bsp1286I	GDGCHC	1 cut(s)	107
Bsp143I	GATC	1 cut(s)	261
BspACI	CCGC	9 cut(s)	18, 21, 40, 42, 63, 78, 84, 94, 417
BspANI	GGCC	2 cut(s)	105, 423
BspCNI	CTCAG	1 cut(s)	241
BspFNI	CGCG	4 cut(s)	42, 63, 84, 96
BspLI	GGNNCC	4 cut(s)	37, 105, 471, 489
BspT104I	TTCGAA	1 cut(s)	182
BssECI	CCNNGG	8 cut(s)	40, 321, 322, 424, 440, 476, 494, 508
BssMI	GATC	1 cut(s)	261
BssT1I	CCWWGG	1 cut(s)	476
Bst2UI	CCWGG	1 cut(s)	323
Bst4CI	ACNGT	2 cut(s)	74, 509
Bst6I	CTCTTC	1 cut(s)	442
BstBI	TTCGAA	1 cut(s)	182
BstC8I	GCNNGC	1 cut(s)	402
BstDEI	CTNAG	1 cut(s)	228
BstDSI	CCRYGG	3 cut(s)	40, 424, 508
BstEII	GGTNACC	1 cut(s)	503
BstENI	CCTNNNNNAGG	1 cut(s)	12
BstFNI	CGCG	4 cut(s)	42, 63, 84, 96
BstKTI	GATC	1 cut(s)	264
BstMBI	GATC	1 cut(s)	261
BstMCI	CGRYCG	2 cut(s)	46, 82
BstMWI	GCNNNNNNNGC	2 cut(s)	102, 522
BstNI	CCWGG	1 cut(s)	323
BstNSI	RCATGY	1 cut(s)	150
BstPI	GGTNACC	1 cut(s)	503
BstSCI	CCNGG	1 cut(s)	321
BstSLI	GKGCMC	1 cut(s)	107
BstUI	CGCG	4 cut(s)	42, 63, 84, 96
BsuRI	GGCC	2 cut(s)	105, 423
BtgI	CCRYGG	3 cut(s)	40, 424, 508
BtgZI	GCGATG	1 cut(s)	111
BtsI	GCAGTG	1 cut(s)	55
BtsIMutI	CAGTG	2 cut(s)	55, 79
Cac8I	GCNNGC	1 cut(s)	402
Cfr13I	GGNCC	5 cut(s)	103, 104, 257, 470, 488
Cfr42I	CCGCGG	1 cut(s)	43
Csp6I	GTAC	1 cut(s)	435
CviAII	CATG	2 cut(s)	143, 147
CviJI	RGCY	8 cut(s)	36, 105, 172, 355, 385, 400, 423, 516
CviKI_1	RGCY	8 cut(s)	36, 105, 172, 355, 385, 400, 423, 516
CviQI	GTAC	1 cut(s)	435
DdeI	CTNAG	1 cut(s)	228
DpnI	GATC	1 cut(s)	263
DpnII	GATC	1 cut(s)	261
Eam1104I	CTCTTC	1 cut(s)	442
EarI	CTCTTC	1 cut(s)	442
EciI	GGCGGA	2 cut(s)	36, 432
Eco130I	CCWWGG	1 cut(s)	476
Eco24I	GRGCYC	1 cut(s)	107
Eco47I	GGWCC	3 cut(s)	257, 470, 488
Eco57I	CTGAAG	1 cut(s)	502
Eco88I	CYCGRG	1 cut(s)	411
Eco91I	GGTNACC	1 cut(s)	503
EcoNI	CCTNNNNNAGG	1 cut(s)	12
EcoO109I	RGGNCCY	4 cut(s)	103, 104, 470, 488
EcoO65I	GGTNACC	1 cut(s)	503
EcoRI	GAATTC	1 cut(s)	178
EcoRII	CCWGG	1 cut(s)	321
EcoT14I	CCWWGG	1 cut(s)	476
EcoT22I	ATGCAT	1 cut(s)	148
EcoT38I	GRGCYC	1 cut(s)	107
ErhI	CCWWGG	1 cut(s)	476
FaeI	CATG	2 cut(s)	146, 150
FaiI	YATR	4 cut(s)	144, 148, 293, 339
FaqI	GGGAC	1 cut(s)	405
FatI	CATG	2 cut(s)	142, 146
FauI	CCCGC	2 cut(s)	47, 56
Fnu4HI	GCNGC	1 cut(s)	19
FriOI	GRGCYC	1 cut(s)	107
Fsp4HI	GCNGC	1 cut(s)	19
GluI	GCNGC	1 cut(s)	19
HaeIII	GGCC	2 cut(s)	105, 423
Hin1II	CATG	2 cut(s)	146, 150
HindIII	AAGCTT	1 cut(s)	353
HinfI	GANTC	2 cut(s)	226, 269
HphI	GGTGA	1 cut(s)	167
Hpy188I	TCNGA	6 cut(s)	32, 115, 231, 261, 441, 495
Hpy188III	TCNNGA	4 cut(s)	166, 175, 365, 411
HpyAV	CCTTC	5 cut(s)	120, 290, 423, 459, 477
HpyCH4III	ACNGT	2 cut(s)	74, 509
HpyCH4V	TGCA	2 cut(s)	146, 217
HpyF10VI	GCNNNNNNNGC	2 cut(s)	102, 522
HpyF3I	CTNAG	1 cut(s)	228
Hsp92II	CATG	2 cut(s)	146, 150
KspI	CCGCGG	1 cut(s)	43
Kzo9I	GATC	1 cut(s)	261
LmnI	GCTCC	1 cut(s)	41
LpnPI	CCDG	5 cut(s)	25, 308, 335, 414, 505
MaeIII	GTNAC	2 cut(s)	163, 503
MalI	GATC	1 cut(s)	263
MboI	GATC	1 cut(s)	261
MboII	GAAGA	5 cut(s)	82, 196, 350, 368, 459
MhlI	GDGCHC	1 cut(s)	107
MluCI	AATT	1 cut(s)	178
MlyI	GAGTC	1 cut(s)	220
Mph1103I	ATGCAT	1 cut(s)	148
MroXI	GAANNNNTTC	1 cut(s)	372
MseI	TTAA	3 cut(s)	69, 198, 285
MslI	CAYNNNNRTG	2 cut(s)	147, 198
MspA1I	CMGCKG	1 cut(s)	42
MspR9I	CCNGG	1 cut(s)	323
MvaI	CCWGG	1 cut(s)	323
MvnI	CGCG	4 cut(s)	42, 63, 84, 96
MwoI	GCNNNNNNNGC	2 cut(s)	102, 522
NdeII	GATC	1 cut(s)	261
NlaIII	CATG	2 cut(s)	146, 150
NlaIV	GGNNCC	4 cut(s)	37, 105, 471, 489
NmuCI	GTSAC	1 cut(s)	163
NsiI	ATGCAT	1 cut(s)	148
NspI	RCATGY	1 cut(s)	150
NspV	TTCGAA	1 cut(s)	182
PaeR7I	CTCGAG	1 cut(s)	411
PasI	CCCWGGG	1 cut(s)	322
PcsI	WCGNNNNNNNCGW	1 cut(s)	173
PdmI	GAANNNNTTC	1 cut(s)	372
PfeI	GAWTC	1 cut(s)	269
PkrI	GCNGC	1 cut(s)	20
PleI	GAGTC	1 cut(s)	220
PpsI	GAGTC	1 cut(s)	220
PpuMI	RGGWCCY	2 cut(s)	470, 488
Psp5II	RGGWCCY	2 cut(s)	470, 488
Psp6I	CCWGG	1 cut(s)	321
PspEI	GGTNACC	1 cut(s)	503
PspGI	CCWGG	1 cut(s)	321
PspN4I	GGNNCC	4 cut(s)	37, 105, 471, 489
PspOMI	GGGCCC	1 cut(s)	103
PspPI	GGNCC	5 cut(s)	103, 104, 257, 470, 488
PspPPI	RGGWCCY	2 cut(s)	470, 488
RsaI	GTAC	1 cut(s)	436
RsaNI	GTAC	1 cut(s)	435
RseI	CAYNNNNRTG	2 cut(s)	147, 198
SacII	CCGCGG	1 cut(s)	43
SaqAI	TTAA	3 cut(s)	69, 198, 285
SatI	GCNGC	1 cut(s)	19
Sau3AI	GATC	1 cut(s)	261
Sau96I	GGNCC	5 cut(s)	103, 104, 257, 470, 488
SchI	GAGTC	1 cut(s)	220
ScrFI	CCNGG	1 cut(s)	323
SduI	GDGCHC	1 cut(s)	107
SetI	ASST	5 cut(s)	10, 129, 174, 357, 454
Sfr274I	CTCGAG	1 cut(s)	411
Sfr303I	CCGCGG	1 cut(s)	43
SfuI	TTCGAA	1 cut(s)	182
SgrBI	CCGCGG	1 cut(s)	43
SinI	GGWCC	3 cut(s)	257, 470, 488
SlaI	CTCGAG	1 cut(s)	411
SmiMI	CAYNNNNRTG	2 cut(s)	147, 198
SmlI	CTYRAG	1 cut(s)	411
SmoI	CTYRAG	1 cut(s)	411
Sse9I	AATT	1 cut(s)	178
SsiI	CCGC	9 cut(s)	18, 21, 40, 42, 63, 78, 84, 94, 417
SspI	AATATT	1 cut(s)	252
StyD4I	CCNGG	1 cut(s)	321
StyI	CCWWGG	1 cut(s)	476
TaaI	ACNGT	2 cut(s)	74, 509
TaqI	TCGA	2 cut(s)	182, 412
TasI	AATT	1 cut(s)	178
TauI	GCSGC	1 cut(s)	21
TfiI	GAWTC	1 cut(s)	269
Tru1I	TTAA	3 cut(s)	69, 198, 285
Tru9I	TTAA	3 cut(s)	69, 198, 285
TscAI	CASTG	2 cut(s)	55, 79
TseFI	GTSAC	1 cut(s)	163
Tsp45I	GTSAC	1 cut(s)	163
TspDTI	ATGAA	3 cut(s)	131, 261, 365
TspRI	CASTG	2 cut(s)	55, 79
VpaK11BI	GGWCC	3 cut(s)	257, 470, 488
XagI	CCTNNNNNAGG	1 cut(s)	12
XapI	RAATTY	1 cut(s)	178
XceI	RCATGY	1 cut(s)	150
XhoI	CTCGAG	1 cut(s)	411
XmnI	GAANNNNTTC	1 cut(s)	372
Zsp2I	ATGCAT	1 cut(s)	148

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.