MD00G1207100.v1.1

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018096
Physical Location & Seq
Forward (+)
50437309 .. 50437734
426 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1207100.v1.1.491

Sequence Viewer

Length: 426 bp
ATGGATAAGGTGTCCATACTCTTGCTTATTTGCTTATTCTTTGTGCTATTTGAAGGTGGTTTGGGTGCGCAAGATCCTTCTGTAACCAAAGTTGTGAACATAACTAATGACTTGGGATCAAGGATCAACTTACTCGTTCATTGCAACTTTCACGGCAAATTCCATGACGAAAACCTCGGCCTACAAACCCTCTCGTTTCACCACTCCTTTTCTCATAGCTTCAAATCCGATGACATCACTCCCAAGACACGATTCTTTTGCTTCTTTTGGTGGAAAAATGGCAATGATGTCTTTGACTTGTATAATCCGCAACGAGATGATCCTCGTTTTGCAGCTAAGTATTCTTGGAGCATTAGACGCAAAGGAGCTTATTCTTATGATGAGAAAAACCATCGATGGGATCTTCTCTACACTTGGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

142

Amino Acids

16.67

Weight (kDa)

8.78

Isoelectric Point (pI)

22.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 32 - 139 1.8e-20 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 69
AciI CCGC 1 cut(s) 308
AclWI GGATC 5 cut(s) 68, 124, 131, 314, 408
AcsI RAATTY 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 397
AgsI TTSAA 2 cut(s) 53, 223
AjuI GAANNNNNNNTTGG 2 cut(s) 236, 268
AluBI AGCT 3 cut(s) 219, 335, 368
AluI AGCT 3 cut(s) 219, 335, 368
AlwI GGATC 5 cut(s) 68, 124, 131, 314, 408
AoxI GGCC 1 cut(s) 178
ApeKI GCWGC 1 cut(s) 332
ApoI RAATTY 1 cut(s) 158
AspLEI GCGC 1 cut(s) 70
AsuHPI GGTGA 1 cut(s) 191
BbvI GCAGC 1 cut(s) 344
BccI CCATC 2 cut(s) 390, 399
BceAI ACGGC 1 cut(s) 169
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BisI GCNGC 1 cut(s) 333
BlsI GCNGC 1 cut(s) 334
Bsa29I ATCGAT 1 cut(s) 394
BsaJI CCNNGG 1 cut(s) 175
Bsc4I CCNNNNNNNGG 1 cut(s) 397
Bse3DI GCAATG 2 cut(s) 139, 289
BseCI ATCGAT 1 cut(s) 394
BseDI CCNNGG 1 cut(s) 175
BseLI CCNNNNNNNGG 1 cut(s) 397
BseMI GCAATG 2 cut(s) 139, 289
BseXI GCAGC 1 cut(s) 344
BshFI GGCC 1 cut(s) 180
BshVI ATCGAT 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 397
BsnI GGCC 1 cut(s) 180
Bsp143I GATC 5 cut(s) 73, 116, 123, 319, 400
BspACI CCGC 1 cut(s) 308
BspANI GGCC 1 cut(s) 180
BspDI ATCGAT 1 cut(s) 394
BspPI GGATC 5 cut(s) 68, 124, 131, 314, 408
BsrDI GCAATG 2 cut(s) 139, 289
BssECI CCNNGG 1 cut(s) 175
BssMI GATC 5 cut(s) 73, 116, 123, 319, 400
BstDEI CTNAG 1 cut(s) 336
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 5 cut(s) 76, 119, 126, 322, 403
BstMBI GATC 5 cut(s) 73, 116, 123, 319, 400
BstMWI GCNNNNNNNGC 1 cut(s) 357
BstV1I GCAGC 1 cut(s) 344
BstX2I RGATCY 2 cut(s) 73, 400
BstYI RGATCY 2 cut(s) 73, 400
Bsu15I ATCGAT 1 cut(s) 394
BsuRI GGCC 1 cut(s) 180
BsuTUI ATCGAT 1 cut(s) 394
CfoI GCGC 1 cut(s) 70
ClaI ATCGAT 1 cut(s) 394
CseI GACGC 1 cut(s) 366
CviAII CATG 1 cut(s) 164
CviJI RGCY 4 cut(s) 180, 219, 335, 368
CviKI_1 RGCY 4 cut(s) 180, 219, 335, 368
DdeI CTNAG 1 cut(s) 336
DpnI GATC 5 cut(s) 75, 118, 125, 321, 402
DpnII GATC 5 cut(s) 73, 116, 123, 319, 400
FaeI CATG 1 cut(s) 167
FaiI YATR 6 cut(s) 17, 101, 165, 216, 303, 378
FatI CATG 1 cut(s) 163
Fnu4HI GCNGC 1 cut(s) 333
Fsp4HI GCNGC 1 cut(s) 333
FspI TGCGCA 1 cut(s) 69
GlaI GCGC 1 cut(s) 69
GluI GCNGC 1 cut(s) 333
HaeIII GGCC 1 cut(s) 180
HgaI GACGC 1 cut(s) 366
HhaI GCGC 1 cut(s) 70
Hin1II CATG 1 cut(s) 167
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HinfI GANTC 1 cut(s) 252
HphI GGTGA 1 cut(s) 191
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 1 cut(s) 229
Hpy8I GTNNAC 1 cut(s) 97
HpyAV CCTTC 2 cut(s) 47, 87
HpyCH4V TGCA 2 cut(s) 144, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 357
HpyF3I CTNAG 1 cut(s) 336
Hsp92II CATG 1 cut(s) 167
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 5 cut(s) 73, 116, 123, 319, 400
LmnI GCTCC 2 cut(s) 348, 365
Lsp1109I GCAGC 1 cut(s) 344
MaeIII GTNAC 1 cut(s) 82
MalI GATC 5 cut(s) 75, 118, 125, 321, 402
MboI GATC 5 cut(s) 73, 116, 123, 319, 400
MboII GAAGA 1 cut(s) 395
MflI RGATCY 2 cut(s) 73, 400
MluCI AATT 1 cut(s) 158
MnlI CCTC 3 cut(s) 185, 200, 333
MwoI GCNNNNNNNGC 1 cut(s) 357
NdeII GATC 5 cut(s) 73, 116, 123, 319, 400
NlaIII CATG 1 cut(s) 167
NmeAIII GCCGAG 1 cut(s) 156
NsbI TGCGCA 1 cut(s) 69
PfeI GAWTC 1 cut(s) 252
PkrI GCNGC 1 cut(s) 334
PsuI RGATCY 2 cut(s) 73, 400
SatI GCNGC 1 cut(s) 333
Sau3AI GATC 5 cut(s) 73, 116, 123, 319, 400
SetI ASST 6 cut(s) 12, 58, 177, 221, 337, 370
Sse9I AATT 1 cut(s) 158
SsiI CCGC 1 cut(s) 308
TaqI TCGA 1 cut(s) 394
TasI AATT 1 cut(s) 158
TfiI GAWTC 1 cut(s) 252
TseI GCWGC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 128
XapI RAATTY 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.