MD06G1010600.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0018096
Physical Location & Seq
Forward (+)
1244001 .. 1244396
396 bp
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UTR
Exon/CDS
Intron
MD06G1010600.v1.1.491

Sequence Viewer

Length: 396 bp
ATGTTGAAGATAACACCACTCGCCTTGCTCATTGTCTTGACATTGCCGACGCCGTTGTTATGCTTCAGTAATGTTAAGAGACATGTGGTTATAACTAATAATTTAGGCCCAGGGATTGTTTTAATGGTTAAATGCAAATCCAAAAACGATGACCTTGGCCGGCACGAGATCCTGTTTCAACACTCGTTTTCATGGAAGTTCAAAACTAATTCAATCAGCACATTGTTCTTTTGCCACATGCGGTGGAATAAGTTCAGCGGAAGCTTTGAGGTATACAAGGCTTCGAGAGATGATAAACGGTGTGCAAACTGTTTGTGGAGAATTGATCAACAAGCAGCTTTTACATATGATGAAGTCAATGGAAAATGGGATATGGGGCATGTGTGGCAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

132

Amino Acids

15.27

Weight (kDa)

9.48

Isoelectric Point (pI)

28.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 28 - 129 1.5e-26 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 92
AccI GTMKAC 1 cut(s) 273
AciI CCGC 2 cut(s) 241, 258
AclWI GGATC 1 cut(s) 163
AcoI YGGCCR 1 cut(s) 157
AcuI CTGAAG 1 cut(s) 49
AcyI GRCGYC 1 cut(s) 50
AflIII ACRYGT 1 cut(s) 82
AgsI TTSAA 4 cut(s) 7, 179, 202, 213
AjnI CCWGG 1 cut(s) 109
AluBI AGCT 2 cut(s) 264, 338
AluI AGCT 2 cut(s) 264, 338
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 163
AoxI GGCC 2 cut(s) 106, 157
ApeKI GCWGC 1 cut(s) 335
Asp700I GAANNNNTTC 1 cut(s) 251
AspS9I GGNCC 1 cut(s) 107
BauI CACGAG 1 cut(s) 164
BbvI GCAGC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 37
BciT130I CCWGG 1 cut(s) 111
BclI TGATCA 1 cut(s) 325
BcoDI GTCTC 1 cut(s) 73
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme1390I CCNGG 1 cut(s) 111
BmgT120I GGNCC 1 cut(s) 107
BmrFI CCNGG 1 cut(s) 111
BsaHI GRCGYC 1 cut(s) 50
BsaJI CCNNGG 3 cut(s) 109, 110, 154
Bse118I RCCGGY 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 41
BseBI CCWGG 1 cut(s) 111
BseDI CCNNGG 3 cut(s) 109, 110, 154
BseMI GCAATG 1 cut(s) 41
BseXI GCAGC 1 cut(s) 347
BshFI GGCC 2 cut(s) 108, 159
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 73
BsnI GGCC 2 cut(s) 108, 159
Bsp143I GATC 2 cut(s) 168, 325
BspACI CCGC 2 cut(s) 241, 258
BspANI GGCC 2 cut(s) 108, 159
BspPI GGATC 1 cut(s) 163
BsrDI GCAATG 1 cut(s) 41
BsrFI RCCGGY 1 cut(s) 159
BssAI RCCGGY 1 cut(s) 159
BssECI CCNNGG 3 cut(s) 109, 110, 154
BssMI GATC 2 cut(s) 168, 325
BssNAI GTATAC 1 cut(s) 274
BssNI GRCGYC 1 cut(s) 50
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 1 cut(s) 154
Bst1107I GTATAC 1 cut(s) 274
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 111
Bst4CI ACNGT 2 cut(s) 300, 311
BstACI GRCGYC 1 cut(s) 50
BstC8I GCNNGC 1 cut(s) 161
BstKTI GATC 2 cut(s) 171, 328
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 2 cut(s) 168, 325
BstMWI GCNNNNNNNGC 1 cut(s) 385
BstNI CCWGG 1 cut(s) 111
BstNSI RCATGY 3 cut(s) 86, 241, 383
BstSCI CCNGG 1 cut(s) 109
BstV1I GCAGC 1 cut(s) 347
BstX2I RGATCY 1 cut(s) 168
BstYI RGATCY 1 cut(s) 168
BstZ17I GTATAC 1 cut(s) 274
BsuRI GGCC 2 cut(s) 108, 159
Cac8I GCNNGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 159
Cfr13I GGNCC 1 cut(s) 107
CseI GACGC 1 cut(s) 58
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviAII CATG 4 cut(s) 83, 192, 238, 380
CviJI RGCY 5 cut(s) 108, 159, 264, 281, 338
CviKI_1 RGCY 5 cut(s) 108, 159, 264, 281, 338
DpnI GATC 2 cut(s) 170, 327
DpnII GATC 2 cut(s) 168, 325
EaeI YGGCCR 1 cut(s) 157
Eco130I CCWWGG 1 cut(s) 154
Eco57I CTGAAG 1 cut(s) 49
EcoRII CCWGG 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 154
ErhI CCWWGG 1 cut(s) 154
FaeI CATG 4 cut(s) 86, 195, 241, 383
FatI CATG 4 cut(s) 82, 191, 237, 379
FauNDI CATATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 325
FblI GTMKAC 1 cut(s) 273
Fnu4HI GCNGC 1 cut(s) 336
Fsp4HI GCNGC 1 cut(s) 336
GluI GCNGC 1 cut(s) 336
HaeIII GGCC 2 cut(s) 108, 159
HapII CCGG 1 cut(s) 160
HgaI GACGC 1 cut(s) 58
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 4 cut(s) 86, 195, 241, 383
HindIII AAGCTT 1 cut(s) 262
HpaII CCGG 1 cut(s) 160
Hpy166II GTNNAC 1 cut(s) 274
Hpy188III TCNNGA 2 cut(s) 37, 285
Hpy8I GTNNAC 1 cut(s) 274
Hpy99I CGWCG 1 cut(s) 52
HpyCH4III ACNGT 2 cut(s) 300, 311
HpyCH4V TGCA 2 cut(s) 135, 305
HpyF10VI GCNNNNNNNGC 1 cut(s) 385
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 4 cut(s) 86, 195, 241, 383
KroI GCCGGC 1 cut(s) 159
KroNI GCCGGC 1 cut(s) 161
Ksp22I TGATCA 1 cut(s) 325
Kzo9I GATC 2 cut(s) 168, 325
LpnPI CCDG 4 cut(s) 96, 123, 173, 185
Lsp1109I GCAGC 1 cut(s) 347
MalI GATC 2 cut(s) 170, 327
MboI GATC 2 cut(s) 168, 325
MboII GAAGA 1 cut(s) 19
MflI RGATCY 1 cut(s) 168
MluCI AATT 3 cut(s) 100, 208, 321
MnlI CCTC 1 cut(s) 262
MroNI GCCGGC 1 cut(s) 159
MroXI GAANNNNTTC 1 cut(s) 251
MseI TTAA 3 cut(s) 75, 122, 129
MspA1I CMGCKG 1 cut(s) 258
MspI CCGG 1 cut(s) 160
MspR9I CCNGG 1 cut(s) 111
MvaI CCWGG 1 cut(s) 111
MwoI GCNNNNNNNGC 1 cut(s) 385
NaeI GCCGGC 1 cut(s) 161
NdeI CATATG 1 cut(s) 346
NdeII GATC 2 cut(s) 168, 325
NgoMIV GCCGGC 1 cut(s) 159
NlaIII CATG 4 cut(s) 86, 195, 241, 383
NspI RCATGY 3 cut(s) 86, 241, 383
PasI CCCWGGG 1 cut(s) 110
PciI ACATGT 1 cut(s) 82
PdiI GCCGGC 1 cut(s) 161
PdmI GAANNNNTTC 1 cut(s) 251
PkrI GCNGC 1 cut(s) 337
PscI ACATGT 1 cut(s) 82
PsiI TTATAA 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 109
PspGI CCWGG 1 cut(s) 109
PspPI GGNCC 1 cut(s) 107
PsuI RGATCY 1 cut(s) 168
SaqAI TTAA 3 cut(s) 75, 122, 129
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 2 cut(s) 168, 325
Sau96I GGNCC 1 cut(s) 107
ScrFI CCNGG 1 cut(s) 111
SetI ASST 4 cut(s) 156, 266, 273, 340
Sse9I AATT 3 cut(s) 100, 208, 321
SsiI CCGC 2 cut(s) 241, 258
StyD4I CCNGG 1 cut(s) 109
StyI CCWWGG 1 cut(s) 154
TaaI ACNGT 2 cut(s) 300, 311
TaqI TCGA 1 cut(s) 284
TasI AATT 3 cut(s) 100, 208, 321
Tru1I TTAA 3 cut(s) 75, 122, 129
Tru9I TTAA 3 cut(s) 75, 122, 129
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 2 cut(s) 180, 366
XceI RCATGY 3 cut(s) 86, 241, 383
XmiI GTMKAC 1 cut(s) 273
XmnI GAANNNNTTC 1 cut(s) 251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.