MD04G1242200.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0018096
Physical Location & Seq
Reverse (-)
31882877 .. 31883296
420 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1242200.v1.1.491

Sequence Viewer

Length: 420 bp
ATGGCAATTGCTAAGGTGTCCATACTCTTGCTTATTTGTTTATTCTTTATGCTATTTGAAGGTGGTTTGGGTGCACAAGATCCTCCTATAACCAGAGTTCTGAATATAACTAATGACTTGGGATCAAGGATTGACTTACTCGTTCATTGCGCATTCCAAGGTGAGAACCTCGGCCTACAAACCCTCTCGTTTCACCACTCCTTTTCTCATAGCTTCAAATCTGATGTCGTCACTCCCAAGACACGACTCTTTTGCCTCTTGTGGTGGCAAAATGGCAGTGATGTCTTTGAATTGTATAACCCTCAACGAGATGATCTCCGTTTTGCAATTAAGTATTCTTGGAGCATTAGACGCAAAGGAGCTTATTCCTATGATGAGAAAAACAACCGTTGGGATCTTCTCTACACTTGGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

140

Amino Acids

16.22

Weight (kDa)

8.82

Isoelectric Point (pI)

30.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 34 - 137 9.9e-21 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 151
AclWI GGATC 3 cut(s) 74, 130, 402
AgsI TTSAA 3 cut(s) 59, 217, 290
AluBI AGCT 2 cut(s) 213, 362
AluI AGCT 2 cut(s) 213, 362
Alw21I GWGCWC 1 cut(s) 76
Alw44I GTGCAC 1 cut(s) 72
AlwI GGATC 3 cut(s) 74, 130, 402
AoxI GGCC 1 cut(s) 172
ApaLI GTGCAC 1 cut(s) 72
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
AspLEI GCGC 1 cut(s) 152
AsuHPI GGTGA 2 cut(s) 173, 185
BaeGI GKGCMC 1 cut(s) 76
Bbv12I GWGCWC 1 cut(s) 76
BcgI CGANNNNNNTGC 2 cut(s) 234, 268
BplI GAGNNNNNCTC 2 cut(s) 300, 332
Bpu10I CCTNAGC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 157, 169
Bse3DI GCAATG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 157, 169
BseMI GCAATG 1 cut(s) 145
BseSI GKGCMC 1 cut(s) 76
BshFI GGCC 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 76
BsmI GAATGC 1 cut(s) 152
BsnI GGCC 1 cut(s) 174
Bsp1286I GDGCHC 1 cut(s) 76
Bsp143I GATC 4 cut(s) 79, 122, 313, 394
BspANI GGCC 1 cut(s) 174
BspPI GGATC 3 cut(s) 74, 130, 402
BsrDI GCAATG 1 cut(s) 145
BssECI CCNNGG 2 cut(s) 157, 169
BssMI GATC 4 cut(s) 79, 122, 313, 394
BssT1I CCWWGG 1 cut(s) 157
Bst4CI ACNGT 1 cut(s) 389
BstDEI CTNAG 1 cut(s) 12
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 4 cut(s) 82, 125, 316, 397
BstMBI GATC 4 cut(s) 79, 122, 313, 394
BstMWI GCNNNNNNNGC 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 76
BstX2I RGATCY 2 cut(s) 79, 394
BstYI RGATCY 2 cut(s) 79, 394
BsuRI GGCC 1 cut(s) 174
BtsI GCAGTG 1 cut(s) 283
BtsIMutI CAGTG 1 cut(s) 283
CfoI GCGC 1 cut(s) 152
CseI GACGC 1 cut(s) 360
CviJI RGCY 3 cut(s) 174, 213, 362
CviKI_1 RGCY 3 cut(s) 174, 213, 362
DdeI CTNAG 1 cut(s) 12
DpnI GATC 4 cut(s) 81, 124, 315, 396
DpnII GATC 4 cut(s) 79, 122, 313, 394
Eco130I CCWWGG 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 157
ErhI CCWWGG 1 cut(s) 157
FaiI YATR 7 cut(s) 23, 50, 89, 107, 210, 297, 372
FspI TGCGCA 1 cut(s) 151
GlaI GCGC 1 cut(s) 151
HaeIII GGCC 1 cut(s) 174
HgaI GACGC 1 cut(s) 360
HhaI GCGC 1 cut(s) 152
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HinfI GANTC 1 cut(s) 246
HphI GGTGA 2 cut(s) 173, 185
Hpy166II GTNNAC 1 cut(s) 74
Hpy188I TCNGA 2 cut(s) 102, 223
Hpy8I GTNNAC 1 cut(s) 74
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 389
HpyCH4V TGCA 2 cut(s) 74, 326
HpyF10VI GCNNNNNNNGC 1 cut(s) 351
HpyF3I CTNAG 1 cut(s) 12
HspAI GCGC 1 cut(s) 150
Kzo9I GATC 4 cut(s) 79, 122, 313, 394
LmnI GCTCC 2 cut(s) 342, 359
LpnPI CCDG 1 cut(s) 106
MaeIII GTNAC 1 cut(s) 229
MalI GATC 4 cut(s) 81, 124, 315, 396
MboI GATC 4 cut(s) 79, 122, 313, 394
MboII GAAGA 1 cut(s) 389
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 2 cut(s) 79, 394
MhlI GDGCHC 1 cut(s) 76
MluCI AATT 3 cut(s) 6, 290, 327
MlyI GAGTC 1 cut(s) 240
MnlI CCTC 5 cut(s) 93, 179, 194, 266, 312
MseI TTAA 1 cut(s) 330
MunI CAATTG 1 cut(s) 6
Mva1269I GAATGC 1 cut(s) 152
MwoI GCNNNNNNNGC 1 cut(s) 351
NdeII GATC 4 cut(s) 79, 122, 313, 394
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 229
NsbI TGCGCA 1 cut(s) 151
PctI GAATGC 1 cut(s) 152
PleI GAGTC 1 cut(s) 240
PpsI GAGTC 1 cut(s) 240
PsuI RGATCY 2 cut(s) 79, 394
SaqAI TTAA 1 cut(s) 330
Sau3AI GATC 4 cut(s) 79, 122, 313, 394
SchI GAGTC 1 cut(s) 240
SduI GDGCHC 1 cut(s) 76
SetI ASST 6 cut(s) 18, 64, 163, 171, 215, 364
Sse9I AATT 3 cut(s) 6, 290, 327
StyI CCWWGG 1 cut(s) 157
TaaI ACNGT 1 cut(s) 389
TasI AATT 3 cut(s) 6, 290, 327
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 134
TspGWI ACGGA 1 cut(s) 308
TspRI CASTG 1 cut(s) 283
VneI GTGCAC 1 cut(s) 72
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.