MD04G1243000.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0018096
Physical Location & Seq
Forward (+)
31957931 .. 31958365
435 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1243000.v1.1.491

Sequence Viewer

Length: 435 bp
ATGGCAATGGATAAGGATAACATACTGTTGCTTATTTGCTTATTCTTTGTGCTATTTGAAGGTGGTTTGGGTGCGCAAGATCCTCCTGTAACTAGAGTTGTGAACATAACTAATGACTTGGGATCACGGATCAAATTAGTCGTTCACTGCAACTTTCACGGCAAATTCCATGATGAAAACCTCGGCCTACAAACCCTCTCGTTTCACCACTCCTTTTCTCATAGCTTCAAATCCGATGACGTCACTCCCAAGACACGACTCTTTTGCTTCTTCTGGTGGAAAAATGGCAATAATGATGTCTTTGACTTGTATAATCCGCAACGAGATGATCCTCGTTTTGCATCTAAGTATTCTTGGAGCATTAGACGCGAAGGAGCTTATTCTTATGATGAGAAAAAACATCAATGGGATCTTCTCTACACTTGGAAAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

145

Amino Acids

17.03

Weight (kDa)

7.77

Isoelectric Point (pI)

23.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 34 - 142 8.1e-20 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 243
Acc16I TGCGCA 1 cut(s) 75
AccII CGCG 1 cut(s) 369
AciI CCGC 1 cut(s) 317
AclWI GGATC 5 cut(s) 74, 130, 137, 323, 417
AcsI RAATTY 1 cut(s) 164
AcyI GRCGYC 1 cut(s) 240
AgsI TTSAA 2 cut(s) 59, 229
AluBI AGCT 2 cut(s) 225, 377
AluI AGCT 2 cut(s) 225, 377
AlwI GGATC 5 cut(s) 74, 130, 137, 323, 417
AoxI GGCC 1 cut(s) 184
ApoI RAATTY 1 cut(s) 164
AspLEI GCGC 1 cut(s) 76
AsuHPI GGTGA 1 cut(s) 197
BceAI ACGGC 1 cut(s) 175
BcgI CGANNNNNNTGC 2 cut(s) 246, 280
BfaI CTAG 1 cut(s) 93
BmsI GCATC 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 181
Bse3DI GCAATG 1 cut(s) 12
BseDI CCNNGG 1 cut(s) 181
BseMI GCAATG 1 cut(s) 12
Bsh1236I CGCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 186
BsnI GGCC 1 cut(s) 186
Bsp143I GATC 5 cut(s) 79, 122, 129, 328, 409
BspACI CCGC 1 cut(s) 317
BspANI GGCC 1 cut(s) 186
BspFNI CGCG 1 cut(s) 369
BspPI GGATC 5 cut(s) 74, 130, 137, 323, 417
BsrDI GCAATG 1 cut(s) 12
BssECI CCNNGG 1 cut(s) 181
BssMI GATC 5 cut(s) 79, 122, 129, 328, 409
BssNI GRCGYC 1 cut(s) 240
Bst4CI ACNGT 1 cut(s) 27
BstACI GRCGYC 1 cut(s) 240
BstDEI CTNAG 1 cut(s) 345
BstFNI CGCG 1 cut(s) 369
BstHHI GCGC 1 cut(s) 76
BstKTI GATC 5 cut(s) 82, 125, 132, 331, 412
BstMBI GATC 5 cut(s) 79, 122, 129, 328, 409
BstMWI GCNNNNNNNGC 1 cut(s) 366
BstUI CGCG 1 cut(s) 369
BstX2I RGATCY 2 cut(s) 79, 409
BstYI RGATCY 2 cut(s) 79, 409
BsuRI GGCC 1 cut(s) 186
BtsI GCAGTG 1 cut(s) 145
BtsIMutI CAGTG 1 cut(s) 145
CfoI GCGC 1 cut(s) 76
CseI GACGC 1 cut(s) 375
CviAII CATG 1 cut(s) 170
CviJI RGCY 3 cut(s) 186, 225, 377
CviKI_1 RGCY 3 cut(s) 186, 225, 377
DdeI CTNAG 1 cut(s) 345
DpnI GATC 5 cut(s) 81, 124, 131, 330, 411
DpnII GATC 5 cut(s) 79, 122, 129, 328, 409
FaeI CATG 1 cut(s) 173
FaiI YATR 6 cut(s) 23, 107, 171, 222, 312, 387
FatI CATG 1 cut(s) 169
FspBI CTAG 1 cut(s) 93
FspI TGCGCA 1 cut(s) 75
GlaI GCGC 1 cut(s) 75
HaeIII GGCC 1 cut(s) 186
HgaI GACGC 1 cut(s) 375
HhaI GCGC 1 cut(s) 76
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 1 cut(s) 173
Hin6I GCGC 1 cut(s) 74
HinP1I GCGC 1 cut(s) 74
HinfI GANTC 1 cut(s) 258
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 2 cut(s) 103, 145
Hpy188I TCNGA 1 cut(s) 235
Hpy8I GTNNAC 2 cut(s) 103, 145
HpyAV CCTTC 2 cut(s) 53, 365
HpyCH4III ACNGT 1 cut(s) 27
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 150, 341
HpyF10VI GCNNNNNNNGC 1 cut(s) 366
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 240
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 1 cut(s) 173
HspAI GCGC 1 cut(s) 74
Kzo9I GATC 5 cut(s) 79, 122, 129, 328, 409
LmnI GCTCC 2 cut(s) 357, 374
LpnPI CCDG 2 cut(s) 99, 259
LweI GCATC 1 cut(s) 350
MaeI CTAG 1 cut(s) 93
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 2 cut(s) 88, 241
MalI GATC 5 cut(s) 81, 124, 131, 330, 411
MboI GATC 5 cut(s) 79, 122, 129, 328, 409
MboII GAAGA 2 cut(s) 262, 404
MflI RGATCY 2 cut(s) 79, 409
MluCI AATT 2 cut(s) 134, 164
MlyI GAGTC 1 cut(s) 252
MnlI CCTC 4 cut(s) 93, 191, 206, 342
MvnI CGCG 1 cut(s) 369
MwoI GCNNNNNNNGC 1 cut(s) 366
NdeII GATC 5 cut(s) 79, 122, 129, 328, 409
NlaIII CATG 1 cut(s) 173
NmeAIII GCCGAG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 241
NsbI TGCGCA 1 cut(s) 75
PleI GAGTC 1 cut(s) 252
PpsI GAGTC 1 cut(s) 252
PsuI RGATCY 2 cut(s) 79, 409
Sau3AI GATC 5 cut(s) 79, 122, 129, 328, 409
SchI GAGTC 1 cut(s) 252
SetI ASST 5 cut(s) 64, 183, 227, 243, 379
SfaNI GCATC 1 cut(s) 350
Sse9I AATT 2 cut(s) 134, 164
SsiI CCGC 1 cut(s) 317
SspMI CTAG 1 cut(s) 93
TaaI ACNGT 1 cut(s) 27
TaiI ACGT 1 cut(s) 243
TasI AATT 2 cut(s) 134, 164
TscAI CASTG 1 cut(s) 152
TseFI GTSAC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 189
TspGWI ACGGA 1 cut(s) 142
TspRI CASTG 1 cut(s) 152
XapI RAATTY 1 cut(s) 164
XspI CTAG 1 cut(s) 93
ZraI GACGTC 1 cut(s) 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.