MD15G1135600.v1.1

Plant self-incompatibility protein S1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0018096
Physical Location & Seq
Reverse (-)
9873850 .. 9874266
417 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1135600.v1.1.491

Sequence Viewer

Length: 417 bp
ATGGCTAAAGTATCAGTAGCATTGCTTACGATCTTATCACTGCTCTCGCTGCTATCAAAATGCAGTTTAGCTGTCGACACTGGTGAAACACGAGTCGTGAACATAACTAATAATTTGGGACCAGGGATCATCCTCACCCTTCAATGCAAATCCAACGACGACGATCTTGGCAAACACGATCTCTCGTTTCAGAGTAACTTCCATTGGGAATTCAAGTCCAATTCTCTCTTCCCAAACACACTATTCTATTGCAACATGTGGTGGCAAAATGTCTACGGAAGCTTTGATGTGTACAAAGCTCAACGAGATGATAATCGATGCGCATCGAAGTGTTGGTGGAGAATTAAACAAACCGGAGCATTTTCTTACGATGAGACCAAGGATCGATGGGATCTTGTGTACATTTGGCAGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Self-incomp_S1

Protein Analysis

139

Amino Acids

16.03

Weight (kDa)

7.6

Isoelectric Point (pI)

21.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 33 - 136 3.9e-30 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 322
AccI GTMKAC 2 cut(s) 75, 273
AclWI GGATC 3 cut(s) 134, 390, 399
AcsI RAATTY 1 cut(s) 209
AfaI GTAC 2 cut(s) 293, 401
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 2 cut(s) 143, 214
AjnI CCWGG 1 cut(s) 121
AjuI GAANNNNNNNTTGG 2 cut(s) 212, 244
AluBI AGCT 3 cut(s) 71, 282, 299
AluI AGCT 3 cut(s) 71, 282, 299
Alw26I GTCTC 1 cut(s) 368
AlwI GGATC 3 cut(s) 134, 390, 399
ApeKI GCWGC 1 cut(s) 49
ApoI RAATTY 1 cut(s) 209
AspLEI GCGC 1 cut(s) 323
AspS9I GGNCC 1 cut(s) 119
AsuHPI GGTGA 2 cut(s) 95, 127
AvaII GGWCC 1 cut(s) 119
BauI CACGAG 1 cut(s) 90
BbvI GCAGC 1 cut(s) 36
BccI CCATC 1 cut(s) 381
BciT130I CCWGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 368
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 1 cut(s) 123
Bme18I GGWCC 1 cut(s) 119
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 308, 332
Bsa29I ATCGAT 2 cut(s) 316, 385
BsaBI GATNNNNATC 2 cut(s) 312, 322
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 2 cut(s) 122, 378
BsaWI WCCGGW 1 cut(s) 353
Bse1I ACTGG 1 cut(s) 85
Bse3DI GCAATG 1 cut(s) 20
Bse8I GATNNNNATC 2 cut(s) 312, 322
BseBI CCWGG 1 cut(s) 123
BseCI ATCGAT 2 cut(s) 316, 385
BseDI CCNNGG 2 cut(s) 122, 378
BseGI GGATG 1 cut(s) 129
BseJI GATNNNNATC 2 cut(s) 312, 322
BseMI GCAATG 1 cut(s) 20
BseNI ACTGG 1 cut(s) 85
BseXI GCAGC 1 cut(s) 36
BshVI ATCGAT 2 cut(s) 316, 385
BsiSI CCGG 1 cut(s) 354
BslFI GGGAC 1 cut(s) 132
BsmAI GTCTC 1 cut(s) 368
BsmFI GGGAC 1 cut(s) 132
Bso31I GGTCTC 1 cut(s) 368
Bsp1407I TGTACA 2 cut(s) 291, 399
Bsp143I GATC 6 cut(s) 30, 126, 163, 178, 382, 391
BspDI ATCGAT 2 cut(s) 316, 385
BspLI GGNNCC 1 cut(s) 120
BspPI GGATC 3 cut(s) 134, 390, 399
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 20
BsrGI TGTACA 2 cut(s) 291, 399
BsrI ACTGG 1 cut(s) 85
BssECI CCNNGG 2 cut(s) 122, 378
BssMI GATC 6 cut(s) 30, 126, 163, 178, 382, 391
BssSI CACGAG 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 378
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 123
Bst6I CTCTTC 1 cut(s) 233
BstAUI TGTACA 2 cut(s) 291, 399
BstF5I GGATG 1 cut(s) 129
BstHHI GCGC 1 cut(s) 323
BstKTI GATC 6 cut(s) 33, 129, 166, 181, 385, 394
BstMAI GTCTC 1 cut(s) 368
BstMBI GATC 6 cut(s) 30, 126, 163, 178, 382, 391
BstMWI GCNNNNNNNGC 1 cut(s) 49
BstNI CCWGG 1 cut(s) 123
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 121
BstV1I GCAGC 1 cut(s) 36
BstX2I RGATCY 1 cut(s) 391
BstYI RGATCY 1 cut(s) 391
Bsu15I ATCGAT 2 cut(s) 316, 385
BsuTUI ATCGAT 2 cut(s) 316, 385
BtsCI GGATG 1 cut(s) 129
BtsI GCAGTG 1 cut(s) 38
BtsIMutI CAGTG 2 cut(s) 38, 78
CfoI GCGC 1 cut(s) 323
Cfr13I GGNCC 1 cut(s) 119
ClaI ATCGAT 2 cut(s) 316, 385
Csp6I GTAC 2 cut(s) 292, 400
CviAII CATG 1 cut(s) 256
CviJI RGCY 4 cut(s) 5, 71, 282, 299
CviKI_1 RGCY 4 cut(s) 5, 71, 282, 299
CviQI GTAC 2 cut(s) 292, 400
DpnI GATC 6 cut(s) 32, 128, 165, 180, 384, 393
DpnII GATC 6 cut(s) 30, 126, 163, 178, 382, 391
Eam1104I CTCTTC 1 cut(s) 233
EarI CTCTTC 1 cut(s) 233
Eco130I CCWWGG 1 cut(s) 378
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 1 cut(s) 119
EcoRI GAATTC 1 cut(s) 209
EcoRII CCWGG 1 cut(s) 121
EcoT14I CCWWGG 1 cut(s) 378
ErhI CCWWGG 1 cut(s) 378
FaeI CATG 1 cut(s) 259
FaiI YATR 2 cut(s) 104, 257
FaqI GGGAC 1 cut(s) 132
FatI CATG 1 cut(s) 255
FblI GTMKAC 2 cut(s) 75, 273
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 1 cut(s) 116
Fsp4HI GCNGC 1 cut(s) 50
FspAI RTGCGCAY 1 cut(s) 322
FspI TGCGCA 1 cut(s) 322
GlaI GCGC 1 cut(s) 322
GluI GCNGC 1 cut(s) 50
HapII CCGG 1 cut(s) 354
HhaI GCGC 1 cut(s) 323
Hin1II CATG 1 cut(s) 259
Hin6I GCGC 1 cut(s) 321
HinP1I GCGC 1 cut(s) 321
HincII GTYRAC 1 cut(s) 76
HindII GTYRAC 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 1 cut(s) 93
HpaII CCGG 1 cut(s) 354
HphI GGTGA 2 cut(s) 95, 127
Hpy166II GTNNAC 5 cut(s) 76, 100, 274, 292, 400
Hpy188I TCNGA 1 cut(s) 192
Hpy188III TCNNGA 1 cut(s) 97
Hpy8I GTNNAC 5 cut(s) 76, 100, 274, 292, 400
Hpy99I CGWCG 2 cut(s) 161, 164
HpyAV CCTTC 1 cut(s) 149
HpyCH4V TGCA 3 cut(s) 63, 147, 252
HpyF10VI GCNNNNNNNGC 1 cut(s) 49
Hsp92II CATG 1 cut(s) 259
HspAI GCGC 1 cut(s) 321
Kzo9I GATC 6 cut(s) 30, 126, 163, 178, 382, 391
LmnI GCTCC 1 cut(s) 356
LpnPI CCDG 4 cut(s) 66, 108, 135, 367
Lsp1109I GCAGC 1 cut(s) 36
LweI GCATC 2 cut(s) 308, 332
MaeIII GTNAC 1 cut(s) 194
MalI GATC 6 cut(s) 32, 128, 165, 180, 384, 393
MboI GATC 6 cut(s) 30, 126, 163, 178, 382, 391
MboII GAAGA 1 cut(s) 220
MflI RGATCY 1 cut(s) 391
MluCI AATT 4 cut(s) 112, 209, 220, 342
MlyI GAGTC 1 cut(s) 102
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 1 cut(s) 143
MseI TTAA 1 cut(s) 345
MslI CAYNNNNRTG 1 cut(s) 328
MspI CCGG 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 123
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 49
NdeII GATC 6 cut(s) 30, 126, 163, 178, 382, 391
NlaIII CATG 1 cut(s) 259
NlaIV GGNNCC 1 cut(s) 120
NsbI TGCGCA 1 cut(s) 322
NspI RCATGY 1 cut(s) 259
PciI ACATGT 1 cut(s) 255
PkrI GCNGC 1 cut(s) 51
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 121
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 119
PsuI RGATCY 1 cut(s) 391
RsaI GTAC 2 cut(s) 293, 401
RsaNI GTAC 2 cut(s) 292, 400
RseI CAYNNNNRTG 1 cut(s) 328
SalI GTCGAC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 6 cut(s) 30, 126, 163, 178, 382, 391
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 1 cut(s) 123
SetI ASST 3 cut(s) 73, 284, 301
SfaNI GCATC 2 cut(s) 308, 332
SinI GGWCC 1 cut(s) 119
SmiMI CAYNNNNRTG 1 cut(s) 328
Sse9I AATT 4 cut(s) 112, 209, 220, 342
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 378
TaqI TCGA 4 cut(s) 75, 316, 326, 385
TasI AATT 4 cut(s) 112, 209, 220, 342
TatI WGTACW 2 cut(s) 291, 399
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 45, 85
TseI GCWGC 1 cut(s) 49
TspGWI ACGGA 1 cut(s) 291
TspRI CASTG 2 cut(s) 45, 85
VpaK11BI GGWCC 1 cut(s) 119
XapI RAATTY 1 cut(s) 209
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 2 cut(s) 75, 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.