MD01G1005800.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0006262
Physical Location & Seq
Forward (+)
2126894 .. 2135703
8810 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1005800.v1.1.491

Sequence Viewer

Length: 378 bp
ATGCTTCTTTTGGATATCAGAGATTTGTACCTATTTTGGATTCGATTGGGATTTGGTTGGAACCGATCTAATCCGGCGTTTCCAGGTCAGTTTTGCTCTGACGACGAGCCCGTAGCTTATTCCCTTGAGAGCGACAAGCTCAAAACGGGGAGTATTGAGAGCATTGGAGGAGAAGGAGAAGAAGACGAAGAGGAAGAGCCCGTGGACGAGCCCGACGATGCCATGGAGGAAGACGCAGTCAACCCCACCGCCGTCTTTTGCATCAGGCTCAAGCAGCCCAAGTCCAATTTGCAGCACAAGATGAGCGTTCCCGAGCTTTGTTACAATTTTAGTACACATATGAAGAGGATTTGTGAAGTTGCTGTGATTTCATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

126

Amino Acids

14.26

Weight (kDa)

4.33

Isoelectric Point (pI)

60.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 249
AfaI GTAC 2 cut(s) 29, 334
AjnI CCWGG 1 cut(s) 82
AluBI AGCT 3 cut(s) 116, 139, 316
AluI AGCT 3 cut(s) 116, 139, 316
Ama87I CYCGRG 1 cut(s) 311
ApeKI GCWGC 2 cut(s) 274, 292
AvaI CYCGRG 1 cut(s) 311
BanII GRGCYC 3 cut(s) 111, 201, 213
BbsI GAAGAC 2 cut(s) 189, 237
BbvI GCAGC 2 cut(s) 286, 304
BceAI ACGGC 1 cut(s) 236
BciT130I CCWGG 1 cut(s) 84
BisI GCNGC 2 cut(s) 275, 293
BlsI GCNGC 2 cut(s) 276, 294
Bme1390I CCNGG 1 cut(s) 84
BmeT110I CYCGRG 1 cut(s) 311
BmiI GGNNCC 1 cut(s) 62
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 2 cut(s) 208, 270
BpiI GAAGAC 2 cut(s) 189, 237
BpuEI CTTGAG 2 cut(s) 146, 254
BsaJI CCNNGG 2 cut(s) 201, 222
BseBI CCWGG 1 cut(s) 84
BseDI CCNNGG 2 cut(s) 201, 222
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 2 cut(s) 286, 304
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 1 cut(s) 74
BsoBI CYCGRG 1 cut(s) 311
Bsp1286I GDGCHC 3 cut(s) 111, 201, 213
Bsp143I GATC 1 cut(s) 65
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 62
BspQI GCTCTTC 1 cut(s) 189
BssECI CCNNGG 2 cut(s) 201, 222
BssMI GATC 1 cut(s) 65
BssT1I CCWWGG 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 84
Bst6I CTCTTC 3 cut(s) 183, 189, 338
BstDSI CCRYGG 2 cut(s) 201, 222
BstKTI GATC 1 cut(s) 68
BstMBI GATC 1 cut(s) 65
BstMWI GCNNNNNNNGC 1 cut(s) 274
BstNI CCWGG 1 cut(s) 84
BstSCI CCNGG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 286, 304
BstV2I GAAGAC 2 cut(s) 189, 237
BtgI CCRYGG 2 cut(s) 201, 222
CseI GACGC 1 cut(s) 242
Csp6I GTAC 2 cut(s) 28, 333
CviAII CATG 2 cut(s) 223, 372
CviJI RGCY 8 cut(s) 109, 116, 139, 199, 211, 268, 277, 316
CviKI_1 RGCY 8 cut(s) 109, 116, 139, 199, 211, 268, 277, 316
CviQI GTAC 2 cut(s) 28, 333
DpnI GATC 1 cut(s) 67
DpnII GATC 1 cut(s) 65
Eam1104I CTCTTC 3 cut(s) 183, 189, 338
EarI CTCTTC 3 cut(s) 183, 189, 338
Eco130I CCWWGG 1 cut(s) 222
Eco24I GRGCYC 3 cut(s) 111, 201, 213
Eco32I GATATC 1 cut(s) 16
Eco88I CYCGRG 1 cut(s) 311
EcoRII CCWGG 1 cut(s) 82
EcoRV GATATC 1 cut(s) 16
EcoT14I CCWWGG 1 cut(s) 222
EcoT38I GRGCYC 3 cut(s) 111, 201, 213
ErhI CCWWGG 1 cut(s) 222
FaeI CATG 2 cut(s) 226, 375
FaiI YATR 4 cut(s) 224, 339, 341, 373
FatI CATG 2 cut(s) 222, 371
FauNDI CATATG 1 cut(s) 339
Fnu4HI GCNGC 2 cut(s) 275, 293
FriOI GRGCYC 3 cut(s) 111, 201, 213
Fsp4HI GCNGC 2 cut(s) 275, 293
GluI GCNGC 2 cut(s) 275, 293
HapII CCGG 1 cut(s) 74
HgaI GACGC 1 cut(s) 242
Hin1II CATG 2 cut(s) 226, 375
HincII GTYRAC 1 cut(s) 241
HindII GTYRAC 1 cut(s) 241
HinfI GANTC 1 cut(s) 40
HpaII CCGG 1 cut(s) 74
Hpy166II GTNNAC 3 cut(s) 205, 241, 335
Hpy188I TCNGA 2 cut(s) 20, 100
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 3 cut(s) 205, 241, 335
Hpy99I CGWCG 2 cut(s) 107, 218
HpyAV CCTTC 1 cut(s) 167
HpyCH4V TGCA 2 cut(s) 261, 292
HpyF10VI GCNNNNNNNGC 1 cut(s) 274
Hsp92II CATG 2 cut(s) 226, 375
Kzo9I GATC 1 cut(s) 65
LguI GCTCTTC 1 cut(s) 189
LpnPI CCDG 4 cut(s) 69, 87, 96, 250
Lsp1109I GCAGC 2 cut(s) 286, 304
LweI GCATC 2 cut(s) 208, 270
MaeIII GTNAC 1 cut(s) 320
MalI GATC 1 cut(s) 67
MboI GATC 1 cut(s) 65
MboII GAAGA 6 cut(s) 191, 194, 200, 206, 242, 355
MhlI GDGCHC 3 cut(s) 111, 201, 213
MluCI AATT 2 cut(s) 286, 325
MmeI TCCRAC 1 cut(s) 38
MnlI CCTC 4 cut(s) 161, 184, 220, 339
MspI CCGG 1 cut(s) 74
MspR9I CCNGG 1 cut(s) 84
MvaI CCWGG 1 cut(s) 84
MwoI GCNNNNNNNGC 1 cut(s) 274
NcoI CCATGG 1 cut(s) 222
NdeI CATATG 1 cut(s) 339
NdeII GATC 1 cut(s) 65
NlaIII CATG 2 cut(s) 226, 375
NlaIV GGNNCC 1 cut(s) 62
PciSI GCTCTTC 1 cut(s) 189
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 213
PfeI GAWTC 1 cut(s) 40
PflFI GACNNNGTC 1 cut(s) 236
PkrI GCNGC 2 cut(s) 276, 294
Psp6I CCWGG 1 cut(s) 82
PspGI CCWGG 1 cut(s) 82
PspN4I GGNNCC 1 cut(s) 62
PsyI GACNNNGTC 1 cut(s) 236
RsaI GTAC 2 cut(s) 29, 334
RsaNI GTAC 2 cut(s) 28, 333
SapI GCTCTTC 1 cut(s) 189
SatI GCNGC 2 cut(s) 275, 293
Sau3AI GATC 1 cut(s) 65
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 3 cut(s) 111, 201, 213
SetI ASST 5 cut(s) 33, 88, 118, 141, 318
SfaNI GCATC 2 cut(s) 208, 270
SmlI CTYRAG 2 cut(s) 125, 269
SmoI CTYRAG 2 cut(s) 125, 269
Sse9I AATT 2 cut(s) 286, 325
SsiI CCGC 1 cut(s) 249
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 1 cut(s) 222
TaqI TCGA 1 cut(s) 43
TasI AATT 2 cut(s) 286, 325
TatI WGTACW 1 cut(s) 332
TfiI GAWTC 1 cut(s) 40
TseI GCWGC 2 cut(s) 274, 292
TspDTI ATGAA 2 cut(s) 356, 360
Tth111I GACNNNGTC 1 cut(s) 236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.