MD01G1037900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008390
Physical Location & Seq
Forward (+)
13083169 .. 13086694
3526 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1037900.v1.1.491

Sequence Viewer

Length: 1701 bp
ATGGATTCGGATGAACAACCTTCTAGCTGCAGTTTCCCATCTCCAAAACTACCAAGGATTCGTTATGACTCATGTTGTAGGCGCTTCTCTAATTTGAGATCAAATTTATCTTCTGCATTGAATTCTACACAGAACATAAAGTTGATCTCAAACGGTTCCTTTTCTCATTGGAGCTTGGCTTGCCTAGGAGCTGGAAGGAAATGTATAAAACAAGGATCAAGCTTCCTAAAAATGAGGGCAAATACTTTGTGCAATCGCTCTTCATTGTATGAAGGAGTGCAAGAAACTTATTCCTGCTTCGTGAGATTGACAAGTTCACCTGAAGAGGATGCAGTACAAATGCAACCAGGATCCGGTGAAACCCGTGTGTTGTTTCCAAATAGCGTTGGTGATGACGTGATCATACAAGTACAAAATTCCAAGGGACAACATTGTGGTTGTGGTCATGCCCTAGTCAAAGTGGCTGCTATTGCTGATGAACTGATACAATTTTCAGAATTCAGTAATTCACTATCTGGCGACAAGATACGATGGTGGCCTATATATAGTAGTGATCTAGAGGATGAACCTGTCGTCAGAATACAATTAAGCATGGATTATTCGAGTATTCCAGTTGAAAATAGTAATCTTAAGTATGGTTCCATTGCAGAAACTGTGGTATATGACTGTGTCTTAGAAATTGCAATGAAAGTTCAACATTTTCAGGAAAGAAATTTGTTATTACATGGCCTGTGGAAGTGGTTATTGACGGAATTTGCATCTTACTATGGAGTATCAAAGGCATACACCAATTTAAGATACCTTTCCTATGTCATGGATGTCGCGACCCCTACTGTAGATTGTCTCACCTTATTATATGATCTGCTAGCAGATGTGAAGGAAATGAGCACAAATCTGTTAAGTCATCAAGAGGCAAAATGGTCTGGCGTGAGGACAGAATATGGAACAACTCATTTTGTTGATTATATGCACACTGAACTGAAGGGAATGCTCGATGAATTTGAAGTCATCATTAGTCGCTGGCCGATATATTCATCTCCTTTGGAGAATGTTATCGCGGATGTTGAAAAGGCAATCATCGAAGCTTTGGACAGGCACTATGATGATGTTTTATCGCCGCTGAAGGATAATCTGACAAACAAAACATTAGGCTTCAAATATGTCCAAAAATTATCCAGACAAAGCATTAGCACATATTCGGTCCCAAATGAGTTAGGAATTCTTTTAAATTCCATGAAGAGGACGCTCGATGTGCTGTGGCCCCATATTGAAAGCCAGTTGATGTCTCGGAGTTCTTGCATCCGCGATGGATATGCCACAGGCGAACATCTTAATGACGTATCTGCTCTACTCAGAACCAAACTTAGAACCTACCGACTAGCCATTGTCGAGAAGCTTTATGAGAATACTAGAGTTCGTAACAAAACAAAGCTGAAGAACATTATTCAACGCTCAAAGGCAGAGAAATCAGTTGTTCAAAGCAGAATGAAGCCTTTGGAGGTTCTTTTGCGGGAGATGATTGATCATCTCCATACTGTTGTTGATCCTACCGTGTTTGTAGAACTCTGCCGAGAGTTCTGGGACCGGTTGGGGCAGGATGTACTGCATATCTCGGAGGATAAGAAAGAGGAGTACAAAGGCTTGCGCGTGGCAGTTTCTCTAAGTTTCTCTTCAGCCGTTGAACTTTCTACTAGATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

567

Amino Acids

64.46

Weight (kDa)

6.42

Isoelectric Point (pI)

47.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0008390)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G65440 AT5G65440 AT5G65440 AT5G65440 AT5G65440 AT5G65440 AT5G65440 AT5G65440 AT5G65440
fragaria_vesca FvH4_1g19880
malus_domestica MD01G1037900.v1.1
prunus_persica Prupe.6G196800_v2.0.a1
pyrus_communis pycom01g06590
rosa_chinensis RchiOBHm_Chr2g0111701
rosa_laevigata RLG00000017918
rosa_multiflora Rmu_sc0000448.1_g000012
rosa_roxburghii Rroxscaffold_2G00132160
rosa_rugosa Rorug02G0175500
rosa_samantha Rh2AG228500 Rh2BG240800 Rh2CG231900 Rh2DG236500
rosa_wichuraiana Rw2G017650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 824, 1058, 1305, 1647
AciI CCGC 4 cut(s) 1058, 1118, 1303, 1510
AclWI GGATC 4 cut(s) 223, 345, 358, 1538
AcoI YGGCCR 1 cut(s) 1022
AcsI RAATTY 9 cut(s) 103, 121, 415, 497, 712, 752, 998, 1218, 1228
AcuI CTGAAG 5 cut(s) 342, 1001, 1142, 1454, 1656
AfaI GTAC 4 cut(s) 336, 411, 1602, 1634
AfiI CCNNNNNNNGG 2 cut(s) 353, 1239
AflII CTTAAG 1 cut(s) 629
AgeI ACCGGT 1 cut(s) 1584
AjiI CACGTC 1 cut(s) 397
AjnI CCWGG 1 cut(s) 346
AluBI AGCT 7 cut(s) 27, 174, 191, 222, 1085, 1396, 1432
AluI AGCT 7 cut(s) 27, 174, 191, 222, 1085, 1396, 1432
Alw21I GWGCWC 1 cut(s) 890
Alw26I GTCTC 2 cut(s) 848, 1290
AlwI GGATC 4 cut(s) 223, 345, 358, 1538
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 4 cut(s) 536, 727, 1022, 1259
ApeKI GCWGC 2 cut(s) 27, 464
ApoI RAATTY 9 cut(s) 103, 121, 415, 497, 712, 752, 998, 1218, 1228
AsiGI ACCGGT 1 cut(s) 1584
AspA2I CCTAGG 1 cut(s) 184
AspLEI GCGC 2 cut(s) 84, 1647
AspS9I GGNCC 3 cut(s) 1201, 1260, 1582
AsuHPI GGTGA 4 cut(s) 309, 368, 401, 838
AsuNHI GCTAGC 1 cut(s) 865
AvaII GGWCC 2 cut(s) 1201, 1582
AvrII CCTAGG 1 cut(s) 184
BaeI ACNNNNGTAYC 2 cut(s) 518, 551
BamHI GGATCC 1 cut(s) 350
Bbv12I GWGCWC 1 cut(s) 890
BbvI GCAGC 2 cut(s) 14, 451
BccI CCATC 3 cut(s) 46, 525, 1301
BceAI ACGGC 1 cut(s) 1661
BcgI CGANNNNNNTGC 2 cut(s) 1295, 1329
BciT130I CCWGG 1 cut(s) 348
BclI TGATCA 2 cut(s) 399, 1522
BcoDI GTCTC 2 cut(s) 848, 1290
BfaI CTAG 8 cut(s) 24, 185, 452, 557, 866, 1379, 1410, 1692
BfmI CTRYAG 2 cut(s) 28, 834
BfoI RGCGCY 1 cut(s) 85
BfrI CTTAAG 1 cut(s) 629
BisI GCNGC 3 cut(s) 28, 465, 1118
BlnI CCTAGG 1 cut(s) 184
BlsI GCNGC 3 cut(s) 29, 466, 1119
Bme1390I CCNGG 1 cut(s) 348
Bme18I GGWCC 2 cut(s) 1201, 1582
BmgBI CACGTC 1 cut(s) 397
BmgT120I GGNCC 3 cut(s) 1201, 1260, 1582
BmiI GGNNCC 6 cut(s) 157, 352, 640, 1203, 1262, 1583
BmrFI CCNGG 1 cut(s) 348
BmsI GCATC 3 cut(s) 319, 767, 1308
BmtI GCTAGC 1 cut(s) 869
BsaJI CCNNGG 3 cut(s) 53, 184, 420
BsaWI WCCGGW 2 cut(s) 353, 1584
Bsc4I CCNNNNNNNGG 2 cut(s) 353, 1239
Bse118I RCCGGY 1 cut(s) 1584
Bse1I ACTGG 2 cut(s) 611, 1276
Bse3DI GCAATG 2 cut(s) 642, 690
BseBI CCWGG 1 cut(s) 348
BseDI CCNNGG 3 cut(s) 53, 184, 420
BseGI GGATG 7 cut(s) 16, 334, 568, 823, 1066, 1299, 1603
BseLI CCNNNNNNNGG 2 cut(s) 353, 1239
BseMI GCAATG 2 cut(s) 642, 690
BseMII CTCAG 1 cut(s) 1366
BseNI ACTGG 2 cut(s) 611, 1276
BseRI GAGGAG 1 cut(s) 1643
BseXI GCAGC 2 cut(s) 14, 451
Bsh1236I CGCG 4 cut(s) 824, 1058, 1305, 1647
BshFI GGCC 4 cut(s) 538, 729, 1024, 1261
BshTI ACCGGT 1 cut(s) 1584
BsiHKAI GWGCWC 1 cut(s) 890
BsiSI CCGG 2 cut(s) 354, 1585
BslFI GGGAC 3 cut(s) 438, 1187, 1595
BslI CCNNNNNNNGG 2 cut(s) 353, 1239
BsmAI GTCTC 2 cut(s) 848, 1290
BsmFI GGGAC 3 cut(s) 438, 1187, 1595
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 4 cut(s) 538, 729, 1024, 1261
Bsp1286I GDGCHC 1 cut(s) 890
Bsp143I GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
Bsp68I TCGCGA 1 cut(s) 824
BspACI CCGC 4 cut(s) 1058, 1118, 1303, 1510
BspANI GGCC 4 cut(s) 538, 729, 1024, 1261
BspCNI CTCAG 1 cut(s) 1365
BspFNI CGCG 4 cut(s) 824, 1058, 1305, 1647
BspLI GGNNCC 6 cut(s) 157, 352, 640, 1203, 1262, 1583
BspMAI CTGCAG 1 cut(s) 32
BspOI GCTAGC 1 cut(s) 869
BspPI GGATC 4 cut(s) 223, 345, 358, 1538
BspQI GCTCTTC 1 cut(s) 265
BspTI CTTAAG 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 642, 690
BsrFI RCCGGY 1 cut(s) 1584
BsrI ACTGG 2 cut(s) 611, 1276
BssAI RCCGGY 1 cut(s) 1584
BssECI CCNNGG 3 cut(s) 53, 184, 420
BssMI GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
BssT1I CCWWGG 3 cut(s) 53, 184, 420
Bst2UI CCWGG 1 cut(s) 348
Bst4CI ACNGT 6 cut(s) 155, 655, 668, 835, 1537, 1552
Bst6I CTCTTC 4 cut(s) 265, 318, 1232, 1675
BstAFI CTTAAG 1 cut(s) 629
BstC8I GCNNGC 4 cut(s) 181, 867, 1022, 1643
BstDEI CTNAG 4 cut(s) 673, 1352, 1364, 1661
BstF5I GGATG 7 cut(s) 16, 334, 568, 823, 1066, 1299, 1603
BstFNI CGCG 4 cut(s) 824, 1058, 1305, 1647
BstH2I RGCGCY 1 cut(s) 85
BstHHI GCGC 2 cut(s) 84, 1647
BstKTI GATC 9 cut(s) 101, 147, 218, 353, 402, 556, 862, 1525, 1546
BstMAI GTCTC 2 cut(s) 848, 1290
BstMBI GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
BstMWI GCNNNNNNNGC 3 cut(s) 180, 470, 1252
BstNI CCWGG 1 cut(s) 348
BstSCI CCNGG 1 cut(s) 346
BstSFI CTRYAG 2 cut(s) 28, 834
BstUI CGCG 4 cut(s) 824, 1058, 1305, 1647
BstV1I GCAGC 2 cut(s) 14, 451
BstX2I RGATCY 1 cut(s) 350
BstYI RGATCY 1 cut(s) 350
BsuRI GGCC 4 cut(s) 538, 729, 1024, 1261
BtgZI GCGATG 1 cut(s) 1320
BtrI CACGTC 1 cut(s) 397
BtsCI GGATG 7 cut(s) 16, 334, 568, 823, 1066, 1299, 1603
BtsIMutI CAGTG 1 cut(s) 972
BtuMI TCGCGA 1 cut(s) 824
Cac8I GCNNGC 4 cut(s) 181, 867, 1022, 1643
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 2 cut(s) 84, 1647
Cfr10I RCCGGY 1 cut(s) 1584
Cfr13I GGNCC 3 cut(s) 1201, 1260, 1582
CseI GACGC 1 cut(s) 1252
Csp6I GTAC 4 cut(s) 335, 410, 1601, 1633
CspAI ACCGGT 1 cut(s) 1584
CviAII CATG 6 cut(s) 72, 446, 592, 725, 814, 1234
CviQI GTAC 4 cut(s) 335, 410, 1601, 1633
DdeI CTNAG 4 cut(s) 673, 1352, 1364, 1661
DpnI GATC 9 cut(s) 100, 146, 217, 352, 401, 555, 861, 1524, 1545
DpnII GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
DraI TTTAAA 1 cut(s) 1227
EaeI YGGCCR 1 cut(s) 1022
Eam1104I CTCTTC 4 cut(s) 265, 318, 1232, 1675
EarI CTCTTC 4 cut(s) 265, 318, 1232, 1675
Eco130I CCWWGG 3 cut(s) 53, 184, 420
Eco47I GGWCC 2 cut(s) 1201, 1582
Eco57I CTGAAG 5 cut(s) 342, 1001, 1142, 1454, 1656
EcoRI GAATTC 3 cut(s) 121, 497, 1218
EcoRII CCWGG 1 cut(s) 346
EcoT14I CCWWGG 3 cut(s) 53, 184, 420
ErhI CCWWGG 3 cut(s) 53, 184, 420
FaeI CATG 6 cut(s) 75, 449, 595, 728, 817, 1237
FalI AAGNNNNNCTT 2 cut(s) 1654, 1686
FaqI GGGAC 3 cut(s) 438, 1187, 1595
FatI CATG 6 cut(s) 71, 445, 591, 724, 813, 1233
FauI CCCGC 1 cut(s) 1503
FbaI TGATCA 2 cut(s) 399, 1522
Fnu4HI GCNGC 3 cut(s) 28, 465, 1118
FokI GGATG 7 cut(s) 23, 341, 575, 830, 1073, 1286, 1610
Fsp4HI GCNGC 3 cut(s) 28, 465, 1118
FspBI CTAG 8 cut(s) 24, 185, 452, 557, 866, 1379, 1410, 1692
GlaI GCGC 2 cut(s) 83, 1646
GluI GCNGC 3 cut(s) 28, 465, 1118
HaeII RGCGCY 1 cut(s) 85
HaeIII GGCC 4 cut(s) 538, 729, 1024, 1261
HapII CCGG 2 cut(s) 354, 1585
HgaI GACGC 1 cut(s) 1252
HhaI GCGC 2 cut(s) 84, 1647
Hin1II CATG 6 cut(s) 75, 449, 595, 728, 817, 1237
Hin6I GCGC 2 cut(s) 82, 1645
HinP1I GCGC 2 cut(s) 82, 1645
HindIII AAGCTT 3 cut(s) 220, 1083, 1394
HinfI GANTC 3 cut(s) 5, 58, 68
HpaII CCGG 2 cut(s) 354, 1585
HphI GGTGA 4 cut(s) 309, 368, 401, 838
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 7 cut(s) 10, 496, 578, 1134, 1290, 1355, 1615
Hpy188III TCNNGA 7 cut(s) 301, 557, 704, 823, 908, 1176, 1390
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 6 cut(s) 30, 189, 266, 871, 976, 1117
HpyCH4III ACNGT 6 cut(s) 155, 655, 668, 835, 1537, 1552
HpyCH4IV ACGT 2 cut(s) 396, 1338
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 470, 1252
HpyF3I CTNAG 4 cut(s) 673, 1352, 1364, 1661
HpySE526I ACGT 2 cut(s) 396, 1338
Hsp92II CATG 6 cut(s) 75, 449, 595, 728, 817, 1237
HspAI GCGC 2 cut(s) 82, 1645
Ksp22I TGATCA 2 cut(s) 399, 1522
Kzo9I GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
LguI GCTCTTC 1 cut(s) 265
LmnI GCTCC 2 cut(s) 171, 188
Lsp1109I GCAGC 2 cut(s) 14, 451
LweI GCATC 3 cut(s) 319, 767, 1308
MaeI CTAG 8 cut(s) 24, 185, 452, 557, 866, 1379, 1410, 1692
MaeII ACGT 2 cut(s) 396, 1338
MaeIII GTNAC 1 cut(s) 1418
MalI GATC 9 cut(s) 100, 146, 217, 352, 401, 555, 861, 1524, 1545
MboI GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
MboII GAAGA 6 cut(s) 102, 252, 335, 1249, 1447, 1662
MflI RGATCY 1 cut(s) 350
MhlI GDGCHC 1 cut(s) 890
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 9 cut(s) 228, 319, 553, 904, 924, 1233, 1492, 1609, 1621
MseI TTAA 6 cut(s) 587, 630, 794, 899, 1226, 1332
MslI CAYNNNNRTG 2 cut(s) 1101, 1332
MspA1I CMGCKG 1 cut(s) 1120
MspCI CTTAAG 1 cut(s) 629
MspI CCGG 2 cut(s) 354, 1585
MspR9I CCNGG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 1 cut(s) 348
MvnI CGCG 4 cut(s) 824, 1058, 1305, 1647
MwoI GCNNNNNNNGC 3 cut(s) 180, 470, 1252
NdeII GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
NheI GCTAGC 1 cut(s) 865
NlaIII CATG 6 cut(s) 75, 449, 595, 728, 817, 1237
NlaIV GGNNCC 6 cut(s) 157, 352, 640, 1203, 1262, 1583
NmeAIII GCCGAG 1 cut(s) 1595
NruI TCGCGA 1 cut(s) 824
PciSI GCTCTTC 1 cut(s) 265
PctI GAATGC 1 cut(s) 993
PfeI GAWTC 2 cut(s) 5, 58
PflFI GACNNNGTC 1 cut(s) 668
PinAI ACCGGT 1 cut(s) 1584
PkrI GCNGC 3 cut(s) 29, 466, 1119
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 346
PspGI CCWGG 1 cut(s) 346
PspN4I GGNNCC 6 cut(s) 157, 352, 640, 1203, 1262, 1583
PspPI GGNCC 3 cut(s) 1201, 1260, 1582
PstI CTGCAG 1 cut(s) 32
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 350
PsyI GACNNNGTC 1 cut(s) 668
RruI TCGCGA 1 cut(s) 824
RsaI GTAC 4 cut(s) 336, 411, 1602, 1634
RsaNI GTAC 4 cut(s) 335, 410, 1601, 1633
RseI CAYNNNNRTG 2 cut(s) 1101, 1332
SapI GCTCTTC 1 cut(s) 265
SaqAI TTAA 6 cut(s) 587, 630, 794, 899, 1226, 1332
SatI GCNGC 3 cut(s) 28, 465, 1118
Sau3AI GATC 9 cut(s) 98, 144, 215, 350, 399, 553, 859, 1522, 1543
Sau96I GGNCC 3 cut(s) 1201, 1260, 1582
SchI GAGTC 1 cut(s) 62
ScrFI CCNGG 1 cut(s) 348
SduI GDGCHC 1 cut(s) 890
SfaNI GCATC 3 cut(s) 319, 767, 1308
SfcI CTRYAG 2 cut(s) 28, 834
SinI GGWCC 2 cut(s) 1201, 1582
SmiMI CAYNNNNRTG 2 cut(s) 1101, 1332
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SsiI CCGC 4 cut(s) 1058, 1118, 1303, 1510
SspMI CTAG 8 cut(s) 24, 185, 452, 557, 866, 1379, 1410, 1692
StyD4I CCNGG 1 cut(s) 346
StyI CCWWGG 3 cut(s) 53, 184, 420
TaaI ACNGT 6 cut(s) 155, 655, 668, 835, 1537, 1552
TaiI ACGT 2 cut(s) 399, 1341
TaqI TCGA 5 cut(s) 602, 993, 1080, 1248, 1389
TaqII GACCGA 1 cut(s) 1189
TatI WGTACW 4 cut(s) 334, 409, 1600, 1632
TauI GCSGC 1 cut(s) 1120
TfiI GAWTC 2 cut(s) 5, 58
Tru1I TTAA 6 cut(s) 587, 630, 794, 899, 1226, 1332
Tru9I TTAA 6 cut(s) 587, 630, 794, 899, 1226, 1332
TscAI CASTG 1 cut(s) 979
TseI GCWGC 2 cut(s) 27, 464
TspGWI ACGGA 1 cut(s) 764
TspRI CASTG 1 cut(s) 979
Tth111I GACNNNGTC 1 cut(s) 668
Vha464I CTTAAG 1 cut(s) 629
VpaK11BI GGWCC 2 cut(s) 1201, 1582
XapI RAATTY 9 cut(s) 103, 121, 415, 497, 712, 752, 998, 1218, 1228
XbaI TCTAGA 1 cut(s) 556
XmaJI CCTAGG 1 cut(s) 184
XspI CTAG 8 cut(s) 24, 185, 452, 557, 866, 1379, 1410, 1692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.