Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0004655

Physical Location & Seq

Genomic Coordinates Forward (+)

13141442 .. 13146814

Gene Structure 5373 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1038200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2520 bp

ATGCTGGTCCCCGGAATTATAGGGTTTCAGGGCCTGCTGCTGCTCCTCATCTTGCTCTCCTTCTTCGTGATTTGTCACAAGTGGAAGAATGCGGCGGCGAAGAAAGAGGAAATCTTGAGGCTTGTGGCTATGGCTTCCGAAGAGACCGCTGTGGCGGAGTTTCAGGCCACGGCTTTTTTCGCTCCAGTGGTGCAGCCGGCGTCCTGGCCGTACCATTGTGCCGTCTGCTATGCCAGTACCACAATGCGCTGCTCTAAATGCAAGGCTGTCCGATACTGTTCTGGTAAGTGTCAAATCATCCATTGGAGACAAGGTCACAAGGATGAATGTCAGCCTTCAATCGATTCCATGCAGTTCCAAGACAGGAGTGATTTTGATGGGGACACAGTTTTACATCACGATGGGAAAGGATCACGTACTAATTTAACTCCATCCAATACCGTATCATGGGATGACAAATCAAGGAAACCTCTTTCTGATGTTGTTGCTCCTGAACAGCCTAGGAATATGTCATCACCCTTAGAGTGTACTAAGCTGGTGAATCATCAAGAGGACATGTCTTGTGAAAACAAGTTATATAAGATGAAATCGAGTCATACTGATAAAGCGGTTGAGTCTACACAAAAATTTCCTAAAGCAAATGCAAACGTATCTGGTGAGGCTCAGCCTGAAAATCTTGGCAGTAAGAAGCAGTCTAGAAGAGCTATTTCAACAGAAAAGTTAGTTACTGATGCCTCTAAGTCCAAAAGCTCATCAAGTAATGACAGGGAAGATGGGTCTAAAGAAGATGGATCTCAGTCAAATAGTGCTTCTGTTGATGAGCCATCTTCGACCACTGAGGGGCATCTCGATTCCATTTCTAGGTCTGAAAAAATCGAAGCTTATCATGCATTGCCTGCAAAATTCAGTAGTATCCCAAGCTTGCCGCAAAATTCTAGTAATGGATTGAAAACATCTATGAGGAAAGTTGTGCAGCAGTTTAGATCCTCAAAACAGCTGAAATGCAACCCTTCAAGTCATGGAGATGAGACTGGAAAATACAAGGTATTGTTTCCATATGACCTGTTCGTGAAACTATACTCCAACAACAGTGCTGATCTTTACCCCTTTGGACTCATGAATTGTGGGAACAGCTGTTATGCTAACGCTGTGCTTCAATGTTTAGCATTTACTCGGCCCCTTGTTTCTTATCTTCTTCAAGGGCTCCATTCAAGAGCATGTCGAAAGAAGGACTGGTGTTTTATCTGTGAGTTTGAAGTTTTGCTTTTGAAGGCAAGAGAAGGGAAGTCTTTACTGTCCCCTATCAGGATACTATCCAAAATACATAAAATTGGAATTCATCTTGGTCAAGGGAGGGAAGAAGATGCTCATGAATTTTTGAGGTATGCTGTTGATACCATGCAATCTGTTTGCCTCAAGGAAGCTGGGGCTGTTGGTCCTTTGGCTGAAGAAACAACTCTAGTAGGCATGACTTTTGGAGGCTATCTCCGATCTAAGATAAAGTGCATGAAGTGCCTTGGAAAATCTGAGCAAATTGAGCGGATGATGGATCTTACAGTTGAAATCGATGGGCAAATTGGAACTCTTGAAGAGGCTCTTGCTCAATTTACGGCTACTGAAACTTTGGACGGGAATAATAGGTACCATTGTAGCAGGTGTAAATCTTATGAGAAAGCCAGAAAGAAATTAACAGTTTTGGAGGCACCAAATATTCTTACAATTGTATTGAAGCGATTTCAGTCTCGTAACTTTGAGAAGCTGAATAAGTCAATAAGGTTCCCAGAGGTTCTCAACATGTCCCCGTATATGGGTGAAACAAGTGATAAATCCCCTTTGTACAGTCTTTATGCTGTGGTTGTTCACTTGGACATTATGAATGCTGCGTATTCGGGCCATTATGTATGTTATGTAAAGAACAATCAGGGGGAGTGGTTCAAGATTGACGACAGCTCAGTTGAACCTGTGGAATTAAAGAGGGTCTTATCTCAAGGAGCATACATGCTACTTTATGCAAGGTGCACCCCACGTCCCCCGGCCTTGCTAGGAAGTAGTGCCATTTCTAATGGTGGGAAATCCAAGAGACGGAACTTGGAAGCTGTTCCTGCAAGCCATACCAAATCCAAGTCGAGATGTAACTCCGCAGTTCCAAGGCCTTACACCAACAATCAATTGCAAAATCCAGATCCAGATGATTGGAGATTCCATGCAACTCATAGAATTCCTGCCATAGATTCATCGAGTGAGAGTTCATCCATATTTAGCTCATCGGATGCAAGTTCTTGTAGCACAGTGAGCACCAAGGAGTCTTCCAGCAATGAAGATTTCTCGGATTACATATTTGGCGAAATGGGACCCAACTGGTACAGCCAATACAGCAGGCATTCGTCCGCTGGGATCACATCTTCATTGTTTCCCAACTTGGATGTAGGTATAAAAGGCCGTAACTTGGAAGGGGAAGGGAATGCCGCAATTTTGTGTAGCGACAGAAGTAGACATCGTAGAAGGTCAACCTTGGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

UCH zf-MYND

Protein Analysis

840

Amino Acids

93.29

Weight (kDa)

8.69

Isoelectric Point (pI)

54.91

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-MYND	PF01753	73 - 110	7.6e-09	MYND finger
UCH	PF00443	370 - 670	2.4e-53	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	371 - 652	9.6e-20	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004655)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G65450 AT5G65450
fragaria_vesca	FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830 FvH4_1g19830
malus_domestica	MD01G1038200.v1.1
prunus_persica	Prupe.6G197100_v2.0.a1 Prupe.6G197100_v2.0.a1 Prupe.6G197100_v2.0.a1 Prupe.6G197100_v2.0.a1 Prupe.6G197100_v2.0.a1
pyrus_communis	pycom01g06620
rosa_chinensis	RchiOBHm_Chr2g0111521
rosa_laevigata	RLG00000017896
rosa_multiflora	Rmu_co8461347.1_g000001 Rmu_sc0005791.1_g000010
rosa_roxburghii	Rroxscaffold_2G00132250
rosa_rugosa	Rorug02G0174700 Rorug02G0174800 Rorug02G0174900
rosa_samantha	Rh2AG227500 Rh2BG239400 Rh2CG231100 Rh2DG235600
rosa_wichuraiana	Rw2G017570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1644
Acc36I	ACCTGC	1 cut(s)	1644
Acc65I	GGTACC	1 cut(s)	1641
AccB1I	GGYRCC	2 cut(s)	1641, 1702
AccBSI	CCGCTC	1 cut(s)	1540
AccI	GTMKAC	2 cut(s)	617, 2491
AclWI	GGATC	6 cut(s)	418, 799, 978, 1557, 2177, 2402
AcoI	YGGCCR	1 cut(s)	206
AcsI	RAATTY	6 cut(s)	626, 902, 931, 1335, 1373, 2217
AcuI	CTGAAG	1 cut(s)	1467
AcyI	GRCGYC	1 cut(s)	200
AfaI	GTAC	7 cut(s)	212, 238, 418, 529, 1643, 1838, 2363
AfiI	CCNNNNNNNGG	8 cut(s)	447, 840, 861, 1305, 1807, 1808, 2082, 2446
AflIII	ACRYGT	2 cut(s)	555, 1794
AjiI	CACGTC	1 cut(s)	2027
AjnI	CCWGG	1 cut(s)	203
Alw21I	GWGCWC	2 cut(s)	2021, 2297
Alw26I	GTCTC	5 cut(s)	137, 301, 1022, 1746, 2074
Alw44I	GTGCAC	1 cut(s)	2017
AlwI	GGATC	6 cut(s)	418, 799, 978, 1557, 2177, 2402
AlwNI	CAGNNNCTG	1 cut(s)	34
AoxI	GGCC	8 cut(s)	31, 165, 206, 1175, 1891, 2034, 2150, 2437
ApaLI	GTGCAC	1 cut(s)	2017
ApeKI	GCWGC	6 cut(s)	37, 40, 193, 249, 973, 1880
ApoI	RAATTY	6 cut(s)	626, 902, 931, 1335, 1373, 2217
Asp700I	GAANNNNTTC	1 cut(s)	2097
Asp718I	GGTACC	1 cut(s)	1641
AspA2I	CCTAGG	1 cut(s)	500
AspLEI	GCGC	1 cut(s)	249
AspS9I	GGNCC	6 cut(s)	7, 31, 1176, 1436, 1891, 2351
AsuC2I	CCSGG	2 cut(s)	12, 2033
AsuHPI	GGTGA	4 cut(s)	507, 550, 668, 1823
AvaII	GGWCC	3 cut(s)	7, 1436, 2351
AvrII	CCTAGG	1 cut(s)	500
BaeGI	GKGCMC	1 cut(s)	2021
BaeI	ACNNNNGTAYC	2 cut(s)	1633, 1666
BanI	GGYRCC	2 cut(s)	1641, 1702
BanII	GRGCYC	1 cut(s)	1206
BbsI	GAAGAC	1 cut(s)	2298
Bbv12I	GWGCWC	2 cut(s)	2021, 2297
BbvI	GCAGC	6 cut(s)	24, 27, 205, 236, 985, 1867
BccI	CCATC	8 cut(s)	371, 395, 439, 767, 782, 832, 1540, 1562
BceAI	ACGGC	5 cut(s)	186, 193, 206, 1626, 2424
BciT130I	CCWGG	1 cut(s)	205
BciVI	GTATCC	2 cut(s)	923, 1302
BcnI	CCSGG	2 cut(s)	12, 2033
BcoDI	GTCTC	5 cut(s)	137, 301, 1022, 1746, 2074
BfaI	CTAG	6 cut(s)	501, 696, 861, 936, 1460, 2042
BfuAI	ACCTGC	1 cut(s)	1644
BfuI	GTATCC	2 cut(s)	923, 1302
BlnI	CCTAGG	1 cut(s)	500
BlpI	GCTNAGC	1 cut(s)	663
Bme1390I	CCNGG	3 cut(s)	12, 205, 2033
Bme18I	GGWCC	3 cut(s)	7, 1436, 2351
BmgBI	CACGTC	1 cut(s)	2027
BmgT120I	GGNCC	6 cut(s)	7, 31, 1176, 1436, 1891, 2351
BmiI	GGNNCC	8 cut(s)	9, 1178, 1205, 1643, 1704, 1778, 2352, 2353
BmrFI	CCNGG	3 cut(s)	12, 205, 2033
BmsI	GCATC	4 cut(s)	721, 853, 1354, 2260
BpiI	GAAGAC	1 cut(s)	2298
BplI	GAGNNNNNCTC	2 cut(s)	1470, 1502
BpmI	CTGGAG	1 cut(s)	168
Bpu1102I	GCTNAGC	1 cut(s)	663
BpuEI	CTTGAG	3 cut(s)	136, 1400, 1971
BpuMI	CCSGG	2 cut(s)	12, 2033
Bsa29I	ATCGAT	2 cut(s)	342, 1566
BsaAI	YACGTR	1 cut(s)	416
BsaHI	GRCGYC	1 cut(s)	200
BsaI	GGTCTC	1 cut(s)	137
BsaJI	CCNNGG	8 cut(s)	10, 168, 500, 1516, 2031, 2147, 2298, 2511
BsaXI	ACNNNNNCTCC	2 cut(s)	298, 328
Bsc4I	CCNNNNNNNGG	8 cut(s)	447, 840, 861, 1305, 1807, 1808, 2082, 2446
Bse118I	RCCGGY	1 cut(s)	196
Bse1I	ACTGG	5 cut(s)	185, 234, 1036, 1238, 2363
Bse3DI	GCAATG	2 cut(s)	890, 2320
BseBI	CCWGG	1 cut(s)	205
BseCI	ATCGAT	2 cut(s)	342, 1566
BseDI	CCNNGG	8 cut(s)	10, 168, 500, 1516, 2031, 2147, 2298, 2511
BseGI	GGATG	8 cut(s)	297, 328, 431, 457, 1548, 2249, 2275, 2428
BseLI	CCNNNNNNNGG	8 cut(s)	447, 840, 861, 1305, 1807, 1808, 2082, 2446
BseMI	GCAATG	2 cut(s)	890, 2320
BseMII	CTCAG	5 cut(s)	677, 809, 828, 1518, 1965
BseNI	ACTGG	5 cut(s)	185, 234, 1036, 1238, 2363
BseRI	GAGGAG	1 cut(s)	35
BseSI	GKGCMC	1 cut(s)	2021
BseXI	GCAGC	6 cut(s)	24, 27, 205, 236, 985, 1867
BseYI	CCCAGC	2 cut(s)	1424, 2390
BsgI	GTGCAG	2 cut(s)	212, 992
BshFI	GGCC	8 cut(s)	33, 167, 208, 1177, 1893, 2036, 2152, 2439
BshNI	GGYRCC	2 cut(s)	1641, 1702
BshVI	ATCGAT	2 cut(s)	342, 1566
BsiHKAI	GWGCWC	2 cut(s)	2021, 2297
BsiSI	CCGG	3 cut(s)	12, 197, 2033
BslFI	GGGAC	5 cut(s)	395, 1282, 1783, 2013, 2364
BslI	CCNNNNNNNGG	8 cut(s)	447, 840, 861, 1305, 1807, 1808, 2082, 2446
BsmAI	GTCTC	5 cut(s)	137, 301, 1022, 1746, 2074
BsmBI	CGTCTC	1 cut(s)	2074
BsmFI	GGGAC	5 cut(s)	395, 1282, 1783, 2013, 2364
BsmI	GAATGC	4 cut(s)	94, 1882, 2380, 2467
BsnI	GGCC	8 cut(s)	33, 167, 208, 1177, 1893, 2036, 2152, 2439
Bso31I	GGTCTC	1 cut(s)	137
Bsp1286I	GDGCHC	3 cut(s)	1206, 2021, 2297
Bsp1407I	TGTACA	1 cut(s)	1836
Bsp143I	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
Bsp1720I	GCTNAGC	1 cut(s)	663
BspANI	GGCC	8 cut(s)	33, 167, 208, 1177, 1893, 2036, 2152, 2439
BspCNI	CTCAG	5 cut(s)	676, 808, 829, 1519, 1964
BspDI	ATCGAT	2 cut(s)	342, 1566
BspHI	TCATGA	2 cut(s)	1116, 1369
BspLI	GGNNCC	8 cut(s)	9, 1178, 1205, 1643, 1704, 1778, 2352, 2353
BspMI	ACCTGC	1 cut(s)	1644
BspPI	GGATC	6 cut(s)	418, 799, 978, 1557, 2177, 2402
BspQI	GCTCTTC	1 cut(s)	694
BspT107I	GGYRCC	2 cut(s)	1641, 1702
BspTNI	GGTCTC	1 cut(s)	137
BsrBI	CCGCTC	1 cut(s)	1540
BsrDI	GCAATG	2 cut(s)	890, 2320
BsrFI	RCCGGY	1 cut(s)	196
BsrGI	TGTACA	1 cut(s)	1836
BsrI	ACTGG	5 cut(s)	185, 234, 1036, 1238, 2363
BssAI	RCCGGY	1 cut(s)	196
BssECI	CCNNGG	8 cut(s)	10, 168, 500, 1516, 2031, 2147, 2298, 2511
BssMI	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
BssNI	GRCGYC	1 cut(s)	200
BssT1I	CCWWGG	5 cut(s)	500, 1516, 2147, 2298, 2511
Bst2UI	CCWGG	1 cut(s)	205
Bst4CI	ACNGT	9 cut(s)	278, 388, 442, 1091, 1296, 1558, 1693, 1841, 2290
Bst6I	CTCTTC	3 cut(s)	135, 694, 1584
BstACI	GRCGYC	1 cut(s)	200
BstAPI	GCANNNNNTGC	2 cut(s)	896, 1512
BstAUI	TGTACA	1 cut(s)	1836
BstBAI	YACGTR	1 cut(s)	416
BstC8I	GCNNGC	6 cut(s)	35, 198, 897, 923, 2107, 2378
BstDEI	CTNAG	9 cut(s)	520, 531, 663, 738, 795, 837, 1494, 1527, 1951
BstDSI	CCRYGG	1 cut(s)	168
BstF5I	GGATG	8 cut(s)	297, 328, 431, 457, 1548, 2249, 2275, 2428
BstHHI	GCGC	1 cut(s)	249
BstKTI	GATC	8 cut(s)	413, 794, 986, 1099, 1493, 1552, 2185, 2397
BstMAI	GTCTC	5 cut(s)	137, 301, 1022, 1746, 2074
BstMBI	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
BstMWI	GCNNNNNNNGC	9 cut(s)	179, 258, 887, 896, 1512, 1537, 2102, 2292, 2373
BstNI	CCWGG	1 cut(s)	205
BstNSI	RCATGY	4 cut(s)	559, 1221, 1798, 2002
BstSCI	CCNGG	3 cut(s)	10, 203, 2031
BstSLI	GKGCMC	1 cut(s)	2021
BstV1I	GCAGC	6 cut(s)	24, 27, 205, 236, 985, 1867
BstV2I	GAAGAC	1 cut(s)	2298
BstX2I	RGATCY	4 cut(s)	791, 983, 1549, 2182
BstXI	CCANNNNNNTGG	1 cut(s)	2193
BstYI	RGATCY	4 cut(s)	791, 983, 1549, 2182
Bsu15I	ATCGAT	2 cut(s)	342, 1566
BsuI	GTATCC	2 cut(s)	923, 1302
BsuRI	GGCC	8 cut(s)	33, 167, 208, 1177, 1893, 2036, 2152, 2439
BsuTUI	ATCGAT	2 cut(s)	342, 1566
BtgI	CCRYGG	1 cut(s)	168
BtrI	CACGTC	1 cut(s)	2027
BtsCI	GGATG	8 cut(s)	297, 328, 431, 457, 1548, 2249, 2275, 2428
BtsIMutI	CAGTG	4 cut(s)	192, 834, 1096, 2295
BveI	ACCTGC	1 cut(s)	1644
Cac8I	GCNNGC	6 cut(s)	35, 198, 897, 923, 2107, 2378
CaiI	CAGNNNCTG	1 cut(s)	34
CciI	TCATGA	2 cut(s)	1116, 1369
CfoI	GCGC	1 cut(s)	249
Cfr10I	RCCGGY	1 cut(s)	196
Cfr13I	GGNCC	6 cut(s)	7, 31, 1176, 1436, 1891, 2351
ClaI	ATCGAT	2 cut(s)	342, 1566
CseI	GACGC	1 cut(s)	189
Csp6I	GTAC	7 cut(s)	211, 237, 417, 528, 1642, 1837, 2362
CviQI	GTAC	7 cut(s)	211, 237, 417, 528, 1642, 1837, 2362
DdeI	CTNAG	9 cut(s)	520, 531, 663, 738, 795, 837, 1494, 1527, 1951
DpnI	GATC	8 cut(s)	412, 793, 985, 1098, 1492, 1551, 2184, 2396
DpnII	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
EaeI	YGGCCR	1 cut(s)	206
Eam1104I	CTCTTC	3 cut(s)	135, 694, 1584
EarI	CTCTTC	3 cut(s)	135, 694, 1584
EciI	GGCGGA	1 cut(s)	170
Eco130I	CCWWGG	5 cut(s)	500, 1516, 2147, 2298, 2511
Eco147I	AGGCCT	1 cut(s)	2152
Eco24I	GRGCYC	1 cut(s)	1206
Eco31I	GGTCTC	1 cut(s)	137
Eco47I	GGWCC	3 cut(s)	7, 1436, 2351
Eco57I	CTGAAG	1 cut(s)	1467
EcoO109I	RGGNCCY	2 cut(s)	31, 2351
EcoRI	GAATTC	2 cut(s)	1335, 2217
EcoRII	CCWGG	1 cut(s)	203
EcoT14I	CCWWGG	5 cut(s)	500, 1516, 2147, 2298, 2511
EcoT22I	ATGCAT	1 cut(s)	892
EcoT38I	GRGCYC	1 cut(s)	1206
ErhI	CCWWGG	5 cut(s)	500, 1516, 2147, 2298, 2511
Esp3I	CGTCTC	1 cut(s)	2074
FalI	AAGNNNNNCTT	6 cut(s)	1248, 1280, 1581, 1613, 1964, 1996
FaqI	GGGAC	5 cut(s)	395, 1282, 1783, 2013, 2364
FauNDI	CATATG	1 cut(s)	1057
FblI	GTMKAC	2 cut(s)	617, 2491
FokI	GGATG	8 cut(s)	284, 335, 418, 464, 1555, 2236, 2282, 2435
FriOI	GRGCYC	1 cut(s)	1206
FspBI	CTAG	6 cut(s)	501, 696, 861, 936, 1460, 2042
GlaI	GCGC	1 cut(s)	248
GsaI	CCCAGC	2 cut(s)	1428, 2394
GsuI	CTGGAG	1 cut(s)	168
HaeIII	GGCC	8 cut(s)	33, 167, 208, 1177, 1893, 2036, 2152, 2439
HapII	CCGG	3 cut(s)	12, 197, 2033
HgaI	GACGC	1 cut(s)	189
HhaI	GCGC	1 cut(s)	249
Hin1I	GRCGYC	1 cut(s)	200
Hin6I	GCGC	1 cut(s)	247
HinP1I	GCGC	1 cut(s)	247
HincII	GTYRAC	1 cut(s)	2508
HindII	GTYRAC	1 cut(s)	2508
HindIII	AAGCTT	2 cut(s)	879, 919
HinfI	GANTC	9 cut(s)	344, 541, 592, 614, 851, 1113, 2199, 2231, 2303
HpaII	CCGG	3 cut(s)	12, 197, 2033
HphI	GGTGA	4 cut(s)	507, 550, 668, 1823
Hpy166II	GTNNAC	6 cut(s)	528, 618, 1861, 2019, 2492, 2508
Hpy188I	TCNGA	8 cut(s)	139, 272, 478, 868, 1490, 1528, 2269, 2329
Hpy8I	GTNNAC	6 cut(s)	528, 618, 1861, 2019, 2492, 2508
HpyAV	CCTTC	9 cut(s)	70, 345, 1020, 1222, 1264, 1274, 2444, 2450, 2496
HpyCH4III	ACNGT	9 cut(s)	278, 388, 442, 1091, 1296, 1558, 1693, 1841, 2290
HpyCH4IV	ACGT	3 cut(s)	415, 648, 2026
HpyF10VI	GCNNNNNNNGC	9 cut(s)	179, 258, 887, 896, 1512, 1537, 2102, 2292, 2373
HpyF3I	CTNAG	9 cut(s)	520, 531, 663, 738, 795, 837, 1494, 1527, 1951
HpySE526I	ACGT	3 cut(s)	415, 648, 2026
Hsp92I	GRCGYC	1 cut(s)	200
HspAI	GCGC	1 cut(s)	247
KflI	GGGWCCC	1 cut(s)	2351
KpnI	GGTACC	1 cut(s)	1645
KroI	GCCGGC	1 cut(s)	196
KroNI	GCCGGC	1 cut(s)	198
Kzo9I	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
LguI	GCTCTTC	1 cut(s)	694
LmnI	GCTCC	5 cut(s)	48, 187, 493, 1209, 1991
Lsp1109I	GCAGC	6 cut(s)	24, 27, 205, 236, 985, 1867
LweI	GCATC	4 cut(s)	721, 853, 1354, 2260
MaeI	CTAG	6 cut(s)	501, 696, 861, 936, 1460, 2042
MaeII	ACGT	3 cut(s)	415, 648, 2026
MaeIII	GTNAC	6 cut(s)	74, 314, 724, 1745, 2132, 2441
MalI	GATC	8 cut(s)	412, 793, 985, 1098, 1492, 1551, 2184, 2396
MbiI	CCGCTC	1 cut(s)	1540
MboI	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
MfeI	CAATTG	2 cut(s)	1719, 2168
MflI	RGATCY	4 cut(s)	791, 983, 1549, 2182
MhlI	GDGCHC	3 cut(s)	1206, 2021, 2297
MlyI	GAGTC	4 cut(s)	601, 623, 1107, 2312
MmeI	TCCRAC	1 cut(s)	1107
Mph1103I	ATGCAT	1 cut(s)	892
MroNI	GCCGGC	1 cut(s)	196
MroXI	GAANNNNTTC	1 cut(s)	2097
MseI	TTAA	3 cut(s)	425, 1688, 1970
MslI	CAYNNNNRTG	3 cut(s)	321, 399, 1023
MspA1I	CMGCKG	4 cut(s)	149, 997, 1134, 2390
MspI	CCGG	3 cut(s)	12, 197, 2033
MspR9I	CCNGG	3 cut(s)	12, 205, 2033
MunI	CAATTG	2 cut(s)	1719, 2168
Mva1269I	GAATGC	4 cut(s)	94, 1882, 2380, 2467
MvaI	CCWGG	1 cut(s)	205
MwoI	GCNNNNNNNGC	9 cut(s)	179, 258, 887, 896, 1512, 1537, 2102, 2292, 2373
NaeI	GCCGGC	1 cut(s)	198
NciI	CCSGG	2 cut(s)	12, 2033
NdeI	CATATG	1 cut(s)	1057
NdeII	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
NgoMIV	GCCGGC	1 cut(s)	196
NlaIV	GGNNCC	8 cut(s)	9, 1178, 1205, 1643, 1704, 1778, 2352, 2353
NmeAIII	GCCGAG	1 cut(s)	1153
NmuCI	GTSAC	2 cut(s)	74, 314
NsiI	ATGCAT	1 cut(s)	892
NspI	RCATGY	4 cut(s)	559, 1221, 1798, 2002
PagI	TCATGA	2 cut(s)	1116, 1369
PaqCI	CACCTGC	1 cut(s)	1644
PceI	AGGCCT	1 cut(s)	2152
PciI	ACATGT	2 cut(s)	555, 1794
PciSI	GCTCTTC	1 cut(s)	694
PctI	GAATGC	4 cut(s)	94, 1882, 2380, 2467
PdiI	GCCGGC	1 cut(s)	198
PdmI	GAANNNNTTC	1 cut(s)	2097
PfeI	GAWTC	5 cut(s)	344, 541, 851, 2199, 2231
PflFI	GACNNNGTC	1 cut(s)	312
PleI	GAGTC	4 cut(s)	600, 622, 1107, 2311
PpsI	GAGTC	4 cut(s)	600, 622, 1107, 2311
Ppu21I	YACGTR	1 cut(s)	416
PpuMI	RGGWCCY	1 cut(s)	2351
PscI	ACATGT	2 cut(s)	555, 1794
Psp5II	RGGWCCY	1 cut(s)	2351
Psp6I	CCWGG	1 cut(s)	203
PspFI	CCCAGC	2 cut(s)	1424, 2390
PspGI	CCWGG	1 cut(s)	203
PspN4I	GGNNCC	8 cut(s)	9, 1178, 1205, 1643, 1704, 1778, 2352, 2353
PspPI	GGNCC	6 cut(s)	7, 31, 1176, 1436, 1891, 2351
PspPPI	RGGWCCY	1 cut(s)	2351
PstNI	CAGNNNCTG	1 cut(s)	34
PsuI	RGATCY	4 cut(s)	791, 983, 1549, 2182
PsyI	GACNNNGTC	1 cut(s)	312
PvuII	CAGCTG	2 cut(s)	997, 1134
RsaI	GTAC	7 cut(s)	212, 238, 418, 529, 1643, 1838, 2363
RsaNI	GTAC	7 cut(s)	211, 237, 417, 528, 1642, 1837, 2362
RseI	CAYNNNNRTG	3 cut(s)	321, 399, 1023
SapI	GCTCTTC	1 cut(s)	694
SaqAI	TTAA	3 cut(s)	425, 1688, 1970
Sau3AI	GATC	8 cut(s)	410, 791, 983, 1096, 1490, 1549, 2182, 2394
Sau96I	GGNCC	6 cut(s)	7, 31, 1176, 1436, 1891, 2351
SchI	GAGTC	4 cut(s)	601, 623, 1107, 2312
ScrFI	CCNGG	3 cut(s)	12, 205, 2033
SduI	GDGCHC	3 cut(s)	1206, 2021, 2297
SfaNI	GCATC	4 cut(s)	721, 853, 1354, 2260
SinI	GGWCC	3 cut(s)	7, 1436, 2351
SmiMI	CAYNNNNRTG	3 cut(s)	321, 399, 1023
SmlI	CTYRAG	3 cut(s)	115, 1415, 1986
SmoI	CTYRAG	3 cut(s)	115, 1415, 1986
SseBI	AGGCCT	1 cut(s)	2152
SspI	AATATT	1 cut(s)	1711
SspMI	CTAG	6 cut(s)	501, 696, 861, 936, 1460, 2042
StuI	AGGCCT	1 cut(s)	2152
StyD4I	CCNGG	3 cut(s)	10, 203, 2031
StyI	CCWWGG	5 cut(s)	500, 1516, 2147, 2298, 2511
TaaI	ACNGT	9 cut(s)	278, 388, 442, 1091, 1296, 1558, 1693, 1841, 2290
TaiI	ACGT	3 cut(s)	418, 651, 2029
TaqI	TCGA	9 cut(s)	342, 590, 830, 849, 876, 1222, 1566, 2126, 2237
TatI	WGTACW	2 cut(s)	527, 1836
TauI	GCSGC	4 cut(s)	95, 98, 928, 2468
TfiI	GAWTC	5 cut(s)	344, 541, 851, 2199, 2231
Tru1I	TTAA	3 cut(s)	425, 1688, 1970
Tru9I	TTAA	3 cut(s)	425, 1688, 1970
TscAI	CASTG	4 cut(s)	192, 841, 1096, 2295
TseFI	GTSAC	2 cut(s)	74, 314
TseI	GCWGC	6 cut(s)	37, 40, 193, 249, 973, 1880
Tsp45I	GTSAC	2 cut(s)	74, 314
TspGWI	ACGGA	1 cut(s)	2098
TspRI	CASTG	4 cut(s)	192, 841, 1096, 2295
Tth111I	GACNNNGTC	1 cut(s)	312
VneI	GTGCAC	1 cut(s)	2017
VpaK11BI	GGWCC	3 cut(s)	7, 1436, 2351
XapI	RAATTY	6 cut(s)	626, 902, 931, 1335, 1373, 2217
XbaI	TCTAGA	1 cut(s)	695
XceI	RCATGY	4 cut(s)	559, 1221, 1798, 2002
XmaJI	CCTAGG	1 cut(s)	500
XmiI	GTMKAC	2 cut(s)	617, 2491
XmnI	GAANNNNTTC	1 cut(s)	2097
XspI	CTAG	6 cut(s)	501, 696, 861, 936, 1460, 2042
Zsp2I	ATGCAT	1 cut(s)	892

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.