Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007686

Physical Location & Seq

Genomic Coordinates Reverse (-)

18226300 .. 18231693

Gene Structure 5394 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1076400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3006 bp

ATGGATGGTATGGAGAATTTTGTCGATAACATGGCTGCAAATCGTGTAGCAGTAAGGAATGAAAAGCTCAAGCCAAAGGAGGTTGATCCTTTTCACAATGTAGCACTGGAGGGTCAAAATCTGCCTGAGTCGCCGGTGGTGTCCACAATGGAGGGCAAGGATTTGAGTAGATGTGTAACTTCTTCATCTGGATTTGAGCCCCGCTGGTCTGCAAATGGCATGGGGCAGGTGGTGGAAGAATTAAAATTGAAACATTATAGGAAACCAGACTCAGCTTCTAGTCAAGAATGTTGGCAAGATCCAGATCCGGCAGTAGCACGAGGTTTCAGAAGCAAGAATTTTCGCGGAGATTCTACGTCACTTGGCAATGATCAGAATCTGTTGAGAGTAAGAGGCGAGCTTAGAGAAATGTCTCCTCGTGTAAGGAGTCTGAAGCCATTGTCAAGCAATCACTCTGAGCAGGAGGCAGATAAACTGTCTGCGTATTTAAGGGCTGAGGACAGCAAGATTATGTCAAACAATATGCTTAGTATTGCCAAGAAGCGACTGAAGACCCAATCTACAAATAGCAATTCTCAGTTACTTGTAAAGGAAATGTCAAAGGGAAAAACAATCAGTAAATTTCCAGAACCTTATGGTGGCTTTGGTAGTCAGGTTGTAGGCCAGAATGATTTAAAGCCCCGTTATGGTTCTGAGGTAGCTAGTGATGCAGAGTTGAAAGCAAATGCCAACAATGATCGGCTTTCTTCACATGTGCAAAATCAGTCTGGCACAAAATCTATCAGTTACGGGATCAGCTTGAGAGAGTGGCTTAAACCTGGGAGTCACAAAGTTGATGTAGCTGAAAGGCTGCTCATATTTAGGCAGATTGTGGAGTTGGTTGATTTTGCACACTCTCAGGGTGTTCCCTTGCAAGACTTACGACCATCGCGCTTCATTTTGTTGCCATCAAATGAGATTAAATACACAGGTGGATCAGCCATCAGAGAACCAATCAGTATTGTGAACCGAGATTTGATTAACAAAAGGCAATCCGAGCAGGATGCAGATGCAGATGCTGAGCGTATTTTAGGTGGAACACAGACAAAAATGAGTGAAGGTAATGAGGACGAATATTTCATAGCTGGTCCAAAAAAATTTGAGTATGGTGAACTCCAGTTCCGAATGAATTCAAGCTACCAAAATAAATTGGTTGCAGTACAGCAAGGGTCAACTTCTGCAATTGTTCAGTTGGAAGAAAAATGGTATACCAGTCCGGAGGAGTTCAGTGAGAGAGGTTGCACACTTTCTTCAAATGTCTATGCCCTTGGGGTCCTTCTTTTTGAGTTGTTGCGTCTCTGTGACTCATGGGAGGTGCATTCCGCATTGATGTGGGATTTGCACGATCGGATTCTGCCACCAAATTTTCTTTCTCAAAATCCACTCGGAGCTGGCTTTTGCTTATGGCTTCTACATCCTGAACCTTTGTCTCGTCCAACAACTAGAGAGATCCTGCAGTCTGAACTGATGGGTGGAAAACAAGAATCGGCTTCTTGTGACGATTTCTCAAAATCTGCTGATGATTTTGATGCAGAATCAGAGGCATTACTTAGTTTTCTCAGCTCATTAAAAGACAAGAAGCAAGGTCATGCCTCTAAGTTAGTTGAAGAAATAAGACGCTTAGAAGAAGATATAAACAAGTTTGGGGGGAGGCCCTTATCAGGAATATCCTCAGATGCTTCCTTAGCACACAAGGAATTTCATAGTGACAGAGAACAGGGTTCAGTGTCAAATATGATTGAGACGCAATTGATGATGAATATCAACCAGTTAGGGGATGCTTACGCATCCACAAGATACCAGATGAGGCTAAAGAAAACTGCTCCTGCAGCACGCCCTGATAAGGAGGTGTTAAACAATCGAGACAGTTGGCGTCATGTGCGAAATGGTAGTCAAGATTCAAGCACCAACCAGAAATCAGGTGATCGGCTTGGAGCCTTTTTTGACGGTTTACCCAAGTTTTCTCGCTTTAGCAAGTTTGAAGTATGTGGGACATTAAGAAACAGAGATCTTCTAAACTCTAGCAATGTGATTTGCTGTTTGAGTTTCGACCGTGATGAGGAGTACATTGCTACGGCTGGAGTATCAAAGAAAATCAAGATCTTTGATTTTGATGCGCTTGTAGACAATTCTTTGGATATCCATTATCCTGTAGTTGAAATGCCACACAAATCTAAGTTGAGCTGTGTTTGCTGGAACAACTATTTTAAGAACTATCTGGCTTCATCGGATTATGATGGAGTCGTACAGATGTGGGATGCAACTACTGGTCAAGGATTTTCTCAGTATGTAGAGCACCAAAGGCGGGCTTGGTGTGTCGACTTCTCTCAAGCTGACCCAAAGAAGTTTGCTAGTGGAAGTGATGACTGTACAGTGAAACTCTGGAGCACTAATGAGAAAAAATCAACAAACACAATCTGGAACGCAGCTAATGTATGTTGTGTTCAGTTCTCTGAATACTCTTCCAATCTCGTGGTTTTTGGATCCGCTGATTACAAAATTTATGGCTATGATCTTCGGAATACTAGAATCCCTTGGTGCACTTTACCTGGTCATGGAAAAGCTGTTAGCTATGTAAAATTTGTAGATGCGGAAACCCTTGTTTCTGCATCTACAGACAACACTCTGAAGCTTTGGGATCTTAACCAATCTACCTCAACCGGATTGTCTCCCAATGCCTGCAGCTTAACATTTAGCGGTCACACTAATGAGAAGAATTTCGTGGGTTTATCTGTTTCAGATGGATACATAGCATGTGGATCAGAAACTAACGAGGTTTATAGTTATTACAGAAGTCTACCGATGCCCATCACTTCACATAAATTTGGAGCAATTGATCCTGTTTCTGGAAGTGAGGTTGGTGATTGCAGCGGGCAGTTTGTCTCGAGTGTCTGTTGGAGAAGAAAGTCTAACATACTTGTTGCTGCCAACTCAAGTGGAACTTTGAAATTATTGCAGATGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009639 GO:0009640 GO:0009648 GO:0009657 GO:0009658 GO:0009791 GO:0009987 GO:0010017 GO:0010099 GO:0010100 GO:0010114 GO:0010218 GO:0010228 GO:0016043 GO:0016604 GO:0022414 GO:0023052 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048519 GO:0048572 GO:0048573 GO:0048575 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071840 GO:0080008 GO:0104004 GO:1902494 GO:1990234 GO:2000026 GO:2000028 GO:2000030 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

Pkinase WD40

Protein Analysis

1002

Amino Acids

111.7

Weight (kDa)

6.17

Isoelectric Point (pI)

44.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WD40_Gbeta	PF25391	685 - 854	2.5e-06	G protein beta WD-40 repeat protein
WD40_CDC20-Fz	PF24807	686 - 892	5.8e-18	CDC20/Fizzy WD40 domain
Beta-prop_WDR3_1st	PF25173	687 - 821	1.1e-12	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	687 - 782	5.6e-07	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	688 - 942	2.6e-31	WDR5 beta-propeller domain
Beta-prop_WDR35_TULP_N	PF24797	689 - 855	2.3e-07	WDR35/TULP4 N-terminal
Beta-prop_EML	PF23409	690 - 896	2.8e-06	Echinoderm microtubule-associated protein first beta-propeller
Beta-prop_RIG_2nd	PF23775	698 - 785	1.1e-07	RIG second beta-propeller
Beta-prop_WDR36-Utp21_1st	PF25171	706 - 892	1.6e-07	WDR36/Utp21 first beta-propeller
Beta-prop_THOC3	PF25174	736 - 935	1.1e-20	THOC3 beta-propeller domain
WD40_Prp19	PF24814	739 - 819	2e-07	Prp19 WD40 domain
WD40_WDHD1_1st	PF24817	751 - 899	2.4e-14	WDHD1 first WD40 domain
Beta-prop_EML_2	PF23414	751 - 898	3.9e-09	Echinoderm microtubule-associated protein second beta-propeller
EIF3I	PF24805	751 - 839	3.2e-07	EIF3I
Beta-prop_WDR36-Utp21_2nd	PF25168	759 - 899	2.2e-08	WDR36/Utp21 second beta-propeller domain
Beta-prop_EML_2	PF23414	784 - 998	4.9e-08	Echinoderm microtubule-associated protein second beta-propeller
Beta-prop_WDR3_1st	PF25173	792 - 941	1.6e-16	WDR3 first beta-propeller domain
Beta-prop_TEP1_2nd	PF25047	794 - 898	1.6e-07	TEP-1 second beta-propeller
Beta-prop_EIPR1	PF23609	814 - 892	1e-06	EIPR1 beta-propeller
WD40_Prp19	PF24814	823 - 935	3.6e-10	Prp19 WD40 domain
Beta-prop_THOC3	PF25174	841 - 999	9.9e-07	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0007686)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46340 AT2G46340
fragaria_vesca	FvH4_7g15970
malus_domestica	MD01G1076400.v1.1 MD07G1145300.v1.1
prunus_persica	Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1
pyrus_communis	pycom01g10530
rosa_chinensis	RchiOBHm_Chr1g0358871
rosa_laevigata	RLG00000027920
rosa_multiflora	Rmu_sc0008757.1_g000013
rosa_roxburghii	Rroxscaffold_4G00297550
rosa_rugosa	Rorug01G0265700 Rorug01G0265800 Rorug01G0265900 Rorug01G0266000
rosa_samantha	Rh1AG279700 Rh1BG246600 Rh1CG263700 Rh1DG275000
rosa_wichuraiana	Rw1G024850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	217
Acc36I	ACCTGC	1 cut(s)	217
AccI	GTMKAC	4 cut(s)	1247, 2163, 2358, 2836
AccII	CGCG	2 cut(s)	345, 931
AccIII	TCCGGA	1 cut(s)	1255
AciI	CCGC	8 cut(s)	202, 345, 1362, 2344, 2526, 2630, 2736, 2910
AcuI	CTGAAG	3 cut(s)	452, 569, 2687
AcyI	GRCGYC	1 cut(s)	1912
AfaI	GTAC	4 cut(s)	1200, 2105, 2286, 2410
AfiI	CCNNNNNNNGG	9 cut(s)	638, 686, 1700, 1813, 1882, 2098, 2344, 2594, 2885
AflIII	ACRYGT	1 cut(s)	751
AgsI	TTSAA	9 cut(s)	250, 718, 1173, 1293, 1646, 1941, 2021, 2198, 2986
AjnI	CCWGG	2 cut(s)	817, 2587
AjuI	GAANNNNNNNTTGG	2 cut(s)	1393, 1425
AloI	GAACNNNNNNTCC	2 cut(s)	1143, 1175
Alw21I	GWGCWC	3 cut(s)	2337, 2429, 2582
Alw26I	GTCTC	7 cut(s)	417, 1340, 1473, 1775, 1896, 2712, 2926
Alw44I	GTGCAC	1 cut(s)	2578
AlwNI	CAGNNNCTG	2 cut(s)	379, 1058
Ama87I	CYCGRG	1 cut(s)	2923
Aor13HI	TCCGGA	1 cut(s)	1255
AoxI	GGCC	2 cut(s)	661, 1691
ApaLI	GTGCAC	1 cut(s)	2578
ApeKI	GCWGC	7 cut(s)	35, 850, 1868, 2465, 2721, 2907, 2963
ArsI	GACNNNNNNTTYG	2 cut(s)	1915, 1947
Asp700I	GAANNNNTTC	2 cut(s)	1168, 2756
AspLEI	GCGC	2 cut(s)	933, 2158
AspS9I	GGNCC	3 cut(s)	1127, 1312, 1692
AsuHPI	GGTGA	3 cut(s)	1160, 1973, 2912
AvaI	CYCGRG	1 cut(s)	2923
AvaII	GGWCC	2 cut(s)	1127, 1312
BaeGI	GKGCMC	1 cut(s)	2582
BamHI	GGATCC	1 cut(s)	2522
BanII	GRGCYC	1 cut(s)	201
BauI	CACGAG	3 cut(s)	318, 417, 2510
BbsI	GAAGAC	1 cut(s)	557
Bbv12I	GWGCWC	3 cut(s)	2337, 2429, 2582
BbvCI	CCTCAGC	1 cut(s)	495
BbvI	GCAGC	7 cut(s)	22, 837, 1880, 2477, 2733, 2919, 2950
BccI	CCATC	8 cut(s)	934, 955, 989, 1501, 2270, 2774, 2855, 2992
BceAI	ACGGC	1 cut(s)	2130
BcgI	CGANNNNNNTGC	2 cut(s)	1025, 1059
BciT130I	CCWGG	2 cut(s)	819, 2589
BciVI	GTATCC	1 cut(s)	2777
BclI	TGATCA	1 cut(s)	370
BcoDI	GTCTC	7 cut(s)	417, 1340, 1473, 1775, 1896, 2712, 2926
BfaI	CTAG	6 cut(s)	279, 702, 1482, 2061, 2391, 2565
BfmI	CTRYAG	5 cut(s)	1493, 1866, 2190, 2652, 2719
BfuAI	ACCTGC	1 cut(s)	217
BfuI	GTATCC	1 cut(s)	2777
BglII	AGATCT	2 cut(s)	2047, 2139
BisI	GCNGC	7 cut(s)	36, 851, 1869, 2466, 2722, 2908, 2964
BlpI	GCTNAGC	1 cut(s)	1059
BlsI	GCNGC	7 cut(s)	37, 852, 1870, 2467, 2723, 2909, 2965
Bme1390I	CCNGG	2 cut(s)	819, 2589
Bme18I	GGWCC	2 cut(s)	1127, 1312
BmeT110I	CYCGRG	1 cut(s)	2923
BmgT120I	GGNCC	3 cut(s)	1127, 1312, 1692
BmiI	GGNNCC	3 cut(s)	1313, 1975, 2524
BmrFI	CCNGG	2 cut(s)	819, 2589
BpiI	GAAGAC	1 cut(s)	557
BpmI	CTGGAG	4 cut(s)	128, 1139, 2139, 2443
Bpu10I	CCTNAGC	2 cut(s)	495, 1723
Bpu1102I	GCTNAGC	1 cut(s)	1059
BpuEI	CTTGAG	4 cut(s)	53, 820, 2352, 2956
BsaBI	GATNNNNATC	4 cut(s)	303, 375, 1799, 2846
BsaHI	GRCGYC	1 cut(s)	1912
BsaJI	CCNNGG	3 cut(s)	818, 1306, 2573
BsaWI	WCCGGW	2 cut(s)	1255, 2699
Bsc4I	CCNNNNNNNGG	9 cut(s)	638, 686, 1700, 1813, 1882, 2098, 2344, 2594, 2885
Bse118I	RCCGGY	1 cut(s)	133
Bse1I	ACTGG	5 cut(s)	111, 1156, 1251, 1807, 2311
Bse3DI	GCAATG	3 cut(s)	373, 2071, 2106
Bse8I	GATNNNNATC	4 cut(s)	303, 375, 1799, 2846
BseAI	TCCGGA	1 cut(s)	1255
BseBI	CCWGG	2 cut(s)	819, 2589
BseDI	CCNNGG	3 cut(s)	818, 1306, 2573
BseGI	GGATG	6 cut(s)	10, 1048, 1453, 1822, 1826, 2302
BseJI	GATNNNNATC	4 cut(s)	303, 375, 1799, 2846
BseLI	CCNNNNNNNGG	9 cut(s)	638, 686, 1700, 1813, 1882, 2098, 2344, 2594, 2885
BseMI	GCAATG	3 cut(s)	373, 2071, 2106
BseNI	ACTGG	5 cut(s)	111, 1156, 1251, 1807, 2311
BseRI	GAGGAG	3 cut(s)	405, 1274, 2114
BseSI	GKGCMC	1 cut(s)	2582
BseXI	GCAGC	7 cut(s)	22, 837, 1880, 2477, 2733, 2919, 2950
Bsh1236I	CGCG	2 cut(s)	345, 931
Bsh1285I	CGRYCG	2 cut(s)	1387, 2092
BshFI	GGCC	2 cut(s)	663, 1693
BsiEI	CGRYCG	2 cut(s)	1387, 2092
BsiHKAI	GWGCWC	3 cut(s)	2337, 2429, 2582
BsiHKCI	CYCGRG	1 cut(s)	2923
BsiSI	CCGG	4 cut(s)	134, 308, 1256, 2700
BslFI	GGGAC	1 cut(s)	2044
BslI	CCNNNNNNNGG	9 cut(s)	638, 686, 1700, 1813, 1882, 2098, 2344, 2594, 2885
BsmAI	GTCTC	7 cut(s)	417, 1340, 1473, 1775, 1896, 2712, 2926
BsmBI	CGTCTC	2 cut(s)	1340, 1775
BsmFI	GGGAC	1 cut(s)	2044
BsmI	GAATGC	1 cut(s)	1357
BsnI	GGCC	2 cut(s)	663, 1693
BsoBI	CYCGRG	1 cut(s)	2923
Bsp1286I	GDGCHC	4 cut(s)	201, 2337, 2429, 2582
Bsp13I	TCCGGA	1 cut(s)	1255
Bsp1407I	TGTACA	1 cut(s)	2408
Bsp1720I	GCTNAGC	1 cut(s)	1059
BspACI	CCGC	8 cut(s)	202, 345, 1362, 2344, 2526, 2630, 2736, 2910
BspANI	GGCC	2 cut(s)	663, 1693
BspEI	TCCGGA	1 cut(s)	1255
BspFNI	CGCG	2 cut(s)	345, 931
BspLI	GGNNCC	3 cut(s)	1313, 1975, 2524
BspMAI	CTGCAG	3 cut(s)	1497, 1870, 2723
BspMI	ACCTGC	1 cut(s)	217
BsrDI	GCAATG	3 cut(s)	373, 2071, 2106
BsrFI	RCCGGY	1 cut(s)	133
BsrGI	TGTACA	1 cut(s)	2408
BsrI	ACTGG	5 cut(s)	111, 1156, 1251, 1807, 2311
BssAI	RCCGGY	1 cut(s)	133
BssECI	CCNNGG	3 cut(s)	818, 1306, 2573
BssNAI	GTATAC	1 cut(s)	1248
BssNI	GRCGYC	1 cut(s)	1912
BssSI	CACGAG	3 cut(s)	318, 417, 2510
BssT1I	CCWWGG	2 cut(s)	1306, 2573
Bst1107I	GTATAC	1 cut(s)	1248
Bst2BI	CACGAG	3 cut(s)	318, 417, 2510
Bst2UI	CCWGG	2 cut(s)	819, 2589
Bst4CI	ACNGT	6 cut(s)	477, 1907, 1988, 2093, 2408, 2413
Bst6I	CTCTTC	1 cut(s)	2506
BstACI	GRCGYC	1 cut(s)	1912
BstAUI	TGTACA	1 cut(s)	2408
BstC8I	GCNNGC	6 cut(s)	398, 1432, 1873, 2346, 2719, 2912
BstF5I	GGATG	6 cut(s)	10, 1048, 1453, 1822, 1826, 2302
BstFNI	CGCG	2 cut(s)	345, 931
BstHHI	GCGC	2 cut(s)	933, 2158
BstMAI	GTCTC	7 cut(s)	417, 1340, 1473, 1775, 1896, 2712, 2926
BstMCI	CGRYCG	2 cut(s)	1387, 2092
BstMWI	GCNNNNNNNGC	8 cut(s)	130, 707, 1036, 1724, 1868, 1918, 2229, 2341
BstNI	CCWGG	2 cut(s)	819, 2589
BstNSI	RCATGY	2 cut(s)	755, 2796
BstSCI	CCNGG	2 cut(s)	817, 2587
BstSFI	CTRYAG	5 cut(s)	1493, 1866, 2190, 2652, 2719
BstSLI	GKGCMC	1 cut(s)	2582
BstUI	CGCG	2 cut(s)	345, 931
BstV1I	GCAGC	7 cut(s)	22, 837, 1880, 2477, 2733, 2919, 2950
BstV2I	GAAGAC	1 cut(s)	557
BstX2I	RGATCY	7 cut(s)	298, 304, 1488, 2047, 2139, 2522, 2677
BstXI	CCANNNNNNTGG	1 cut(s)	2512
BstYI	RGATCY	7 cut(s)	298, 304, 1488, 2047, 2139, 2522, 2677
BstZ17I	GTATAC	1 cut(s)	1248
BsuI	GTATCC	1 cut(s)	2777
BsuRI	GGCC	2 cut(s)	663, 1693
BtgZI	GCGATG	1 cut(s)	912
BtsCI	GGATG	6 cut(s)	10, 1048, 1453, 1822, 1826, 2302
BtsIMutI	CAGTG	4 cut(s)	104, 1273, 1770, 2418
BveI	ACCTGC	1 cut(s)	217
Cac8I	GCNNGC	6 cut(s)	398, 1432, 1873, 2346, 2719, 2912
CaiI	CAGNNNCTG	2 cut(s)	379, 1058
CfoI	GCGC	2 cut(s)	933, 2158
Cfr10I	RCCGGY	1 cut(s)	133
Cfr13I	GGNCC	3 cut(s)	1127, 1312, 1692
CseI	GACGC	4 cut(s)	1322, 1665, 1792, 1901
CsiI	ACCWGGT	1 cut(s)	2587
Csp6I	GTAC	4 cut(s)	1199, 2104, 2285, 2409
CspCI	CAANNNNNGTGG	2 cut(s)	2956, 2991
CviAII	CATG	8 cut(s)	31, 220, 752, 1347, 1628, 1916, 2594, 2793
CviQI	GTAC	4 cut(s)	1199, 2104, 2285, 2409
DraI	TTTAAA	1 cut(s)	675
Eam1104I	CTCTTC	1 cut(s)	2506
EarI	CTCTTC	1 cut(s)	2506
Eco130I	CCWWGG	2 cut(s)	1306, 2573
Eco24I	GRGCYC	1 cut(s)	201
Eco32I	GATATC	1 cut(s)	2179
Eco47I	GGWCC	2 cut(s)	1127, 1312
Eco57I	CTGAAG	3 cut(s)	452, 569, 2687
Eco88I	CYCGRG	1 cut(s)	2923
EcoO109I	RGGNCCY	2 cut(s)	1312, 1692
EcoRI	GAATTC	1 cut(s)	1168
EcoRII	CCWGG	2 cut(s)	817, 2587
EcoRV	GATATC	1 cut(s)	2179
EcoT14I	CCWWGG	2 cut(s)	1306, 2573
EcoT38I	GRGCYC	1 cut(s)	201
ErhI	CCWWGG	2 cut(s)	1306, 2573
Esp3I	CGTCTC	2 cut(s)	1340, 1775
FaeI	CATG	8 cut(s)	34, 223, 755, 1350, 1631, 1919, 2597, 2796
FalI	AAGNNNNNCTT	4 cut(s)	2332, 2364, 2965, 2997
FaqI	GGGAC	1 cut(s)	2044
FatI	CATG	8 cut(s)	30, 219, 751, 1346, 1627, 1915, 2593, 2792
FauI	CCCGC	3 cut(s)	209, 2337, 2903
FbaI	TGATCA	1 cut(s)	370
FblI	GTMKAC	4 cut(s)	1247, 2163, 2358, 2836
Fnu4HI	GCNGC	7 cut(s)	36, 851, 1869, 2466, 2722, 2908, 2964
FokI	GGATG	6 cut(s)	17, 1055, 1440, 1813, 1829, 2309
FriOI	GRGCYC	1 cut(s)	201
Fsp4HI	GCNGC	7 cut(s)	36, 851, 1869, 2466, 2722, 2908, 2964
FspBI	CTAG	6 cut(s)	279, 702, 1482, 2061, 2391, 2565
GlaI	GCGC	2 cut(s)	932, 2157
GluI	GCNGC	7 cut(s)	36, 851, 1869, 2466, 2722, 2908, 2964
GsuI	CTGGAG	4 cut(s)	128, 1139, 2139, 2443
HaeIII	GGCC	2 cut(s)	663, 1693
HapII	CCGG	4 cut(s)	134, 308, 1256, 2700
HgaI	GACGC	4 cut(s)	1322, 1665, 1792, 1901
HhaI	GCGC	2 cut(s)	933, 2158
Hin1I	GRCGYC	1 cut(s)	1912
Hin1II	CATG	8 cut(s)	34, 223, 755, 1350, 1631, 1919, 2597, 2796
Hin6I	GCGC	2 cut(s)	931, 2156
HinP1I	GCGC	2 cut(s)	931, 2156
HincII	GTYRAC	2 cut(s)	1212, 2359
HindII	GTYRAC	2 cut(s)	1212, 2359
HindIII	AAGCTT	1 cut(s)	2669
HpaII	CCGG	4 cut(s)	134, 308, 1256, 2700
HphI	GGTGA	3 cut(s)	1160, 1973, 2912
HpyAV	CCTTC	2 cut(s)	1091, 1325
HpyCH4III	ACNGT	6 cut(s)	477, 1907, 1988, 2093, 2408, 2413
HpyCH4IV	ACGT	1 cut(s)	356
HpyF10VI	GCNNNNNNNGC	8 cut(s)	130, 707, 1036, 1724, 1868, 1918, 2229, 2341
HpySE526I	ACGT	1 cut(s)	356
Hsp92I	GRCGYC	1 cut(s)	1912
Hsp92II	CATG	8 cut(s)	34, 223, 755, 1350, 1631, 1919, 2597, 2796
HspAI	GCGC	2 cut(s)	931, 2156
Kpn2I	TCCGGA	1 cut(s)	1255
Ksp22I	TGATCA	1 cut(s)	370
LmnI	GCTCC	5 cut(s)	1427, 1867, 1973, 2424, 2867
Lsp1109I	GCAGC	7 cut(s)	22, 837, 1880, 2477, 2733, 2919, 2950
MabI	ACCWGGT	1 cut(s)	2587
MaeI	CTAG	6 cut(s)	279, 702, 1482, 2061, 2391, 2565
MaeII	ACGT	1 cut(s)	356
MaeIII	GTNAC	9 cut(s)	175, 357, 579, 785, 824, 1340, 1535, 1745, 2738
MfeI	CAATTG	3 cut(s)	1221, 1787, 2871
MflI	RGATCY	7 cut(s)	298, 304, 1488, 2047, 2139, 2522, 2677
MhlI	GDGCHC	4 cut(s)	201, 2337, 2429, 2582
MlyI	GAGTC	6 cut(s)	137, 263, 436, 832, 1337, 2289
MmeI	TCCRAC	3 cut(s)	1212, 1499, 2915
MroI	TCCGGA	1 cut(s)	1255
MroXI	GAANNNNTTC	2 cut(s)	1168, 2756
MslI	CAYNNNNRTG	2 cut(s)	1369, 2745
MspA1I	CMGCKG	3 cut(s)	204, 2528, 2910
MspI	CCGG	4 cut(s)	134, 308, 1256, 2700
MspR9I	CCNGG	2 cut(s)	819, 2589
MunI	CAATTG	3 cut(s)	1221, 1787, 2871
Mva1269I	GAATGC	1 cut(s)	1357
MvaI	CCWGG	2 cut(s)	819, 2589
MvnI	CGCG	2 cut(s)	345, 931
MwoI	GCNNNNNNNGC	8 cut(s)	130, 707, 1036, 1724, 1868, 1918, 2229, 2341
NlaIII	CATG	8 cut(s)	34, 223, 755, 1350, 1631, 1919, 2597, 2796
NlaIV	GGNNCC	3 cut(s)	1313, 1975, 2524
NmuCI	GTSAC	6 cut(s)	357, 824, 1340, 1535, 1745, 2738
NspI	RCATGY	2 cut(s)	755, 2796
PaeR7I	CTCGAG	1 cut(s)	2923
PaqCI	CACCTGC	1 cut(s)	217
PciI	ACATGT	1 cut(s)	751
PctI	GAATGC	1 cut(s)	1357
PdmI	GAANNNNTTC	2 cut(s)	1168, 2756
PfeI	GAWTC	7 cut(s)	350, 376, 1390, 1523, 1574, 1937, 2568
PkrI	GCNGC	7 cut(s)	37, 852, 1870, 2467, 2723, 2909, 2965
Ple19I	CGATCG	1 cut(s)	1387
PleI	GAGTC	6 cut(s)	136, 263, 435, 831, 1337, 2288
PpsI	GAGTC	6 cut(s)	136, 263, 435, 831, 1337, 2288
PpuMI	RGGWCCY	1 cut(s)	1312
PscI	ACATGT	1 cut(s)	751
Psp5II	RGGWCCY	1 cut(s)	1312
Psp6I	CCWGG	2 cut(s)	817, 2587
PspGI	CCWGG	2 cut(s)	817, 2587
PspN4I	GGNNCC	3 cut(s)	1313, 1975, 2524
PspPI	GGNCC	3 cut(s)	1127, 1312, 1692
PspPPI	RGGWCCY	1 cut(s)	1312
PsrI	GAACNNNNNNTAC	2 cut(s)	2466, 2498
PstI	CTGCAG	3 cut(s)	1497, 1870, 2723
PstNI	CAGNNNCTG	2 cut(s)	379, 1058
PsuI	RGATCY	7 cut(s)	298, 304, 1488, 2047, 2139, 2522, 2677
PvuI	CGATCG	1 cut(s)	1387
RsaI	GTAC	4 cut(s)	1200, 2105, 2286, 2410
RsaNI	GTAC	4 cut(s)	1199, 2104, 2285, 2409
RseI	CAYNNNNRTG	2 cut(s)	1369, 2745
SalI	GTCGAC	1 cut(s)	2357
SatI	GCNGC	7 cut(s)	36, 851, 1869, 2466, 2722, 2908, 2964
Sau96I	GGNCC	3 cut(s)	1127, 1312, 1692
SchI	GAGTC	6 cut(s)	137, 263, 436, 832, 1337, 2289
ScrFI	CCNGG	2 cut(s)	819, 2589
SduI	GDGCHC	4 cut(s)	201, 2337, 2429, 2582
SexAI	ACCWGGT	1 cut(s)	2587
SfcI	CTRYAG	5 cut(s)	1493, 1866, 2190, 2652, 2719
Sfr274I	CTCGAG	1 cut(s)	2923
SgrAI	CRCCGGYG	1 cut(s)	133
SinI	GGWCC	2 cut(s)	1127, 1312
SlaI	CTCGAG	1 cut(s)	2923
SmiMI	CAYNNNNRTG	2 cut(s)	1369, 2745
SmlI	CTYRAG	5 cut(s)	68, 799, 2367, 2923, 2971
SmoI	CTYRAG	5 cut(s)	68, 799, 2367, 2923, 2971
SsiI	CCGC	8 cut(s)	202, 345, 1362, 2344, 2526, 2630, 2736, 2910
SspI	AATATT	1 cut(s)	1115
SspMI	CTAG	6 cut(s)	279, 702, 1482, 2061, 2391, 2565
StyD4I	CCNGG	2 cut(s)	817, 2587
StyI	CCWWGG	2 cut(s)	1306, 2573
TaaI	ACNGT	6 cut(s)	477, 1907, 1988, 2093, 2408, 2413
TaiI	ACGT	1 cut(s)	359
TaqI	TCGA	5 cut(s)	24, 1900, 2088, 2358, 2924
TatI	WGTACW	3 cut(s)	1198, 2103, 2408
TfiI	GAWTC	7 cut(s)	350, 376, 1390, 1523, 1574, 1937, 2568
TscAI	CASTG	4 cut(s)	111, 1273, 1770, 2418
TseFI	GTSAC	6 cut(s)	357, 824, 1340, 1535, 1745, 2738
TseI	GCWGC	7 cut(s)	35, 850, 1868, 2465, 2721, 2907, 2963
Tsp45I	GTSAC	6 cut(s)	357, 824, 1340, 1535, 1745, 2738
TspDTI	ATGAA	8 cut(s)	75, 174, 925, 1108, 1181, 1730, 1811, 2253
TspRI	CASTG	4 cut(s)	111, 1273, 1770, 2418
VneI	GTGCAC	1 cut(s)	2578
VpaK11BI	GGWCC	2 cut(s)	1127, 1312
XceI	RCATGY	2 cut(s)	755, 2796
XhoI	CTCGAG	1 cut(s)	2923
XmiI	GTMKAC	4 cut(s)	1247, 2163, 2358, 2836
XmnI	GAANNNNTTC	2 cut(s)	1168, 2756
XspI	CTAG	6 cut(s)	279, 702, 1482, 2061, 2391, 2565

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.