Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0007686

Physical Location & Seq

Genomic Coordinates Reverse (-)

21171769 .. 21176675

Gene Structure 4907 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1145300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2979 bp

ATGGATGGTGTTGAGAGTTTCGCCGATAGCAAGACTGCAAATGGTGTAGCAGTAAGGAATGAAAAGCTCAAGCCAAAGGATGTTGATCCTTTTCACAAACTGGAGGGTCAAAATCTGTTGGAGTTGCTGGTGGTGTCCACACTGGAGGGAAAGGATTTGAGTAGGTGTGTAACTTCTTCATCTGGGTTTAAACCCCCGTGGCCTGCAAATGGCACGGCACATCTGGTAGAAGAATTAAATTTGAAACATTATAGAAAACAAGACTCAGCTTTGCTTAGTTCTAGTCAAGAATGTTGGCAAGGTACAGATCCGGCAGTAGCACGAGGTTTCAGAAGTAAGAATTTTCGTGGTAAGTCTACATCACAGGGAAATGATCAGAATTTGTTGAGAGTAAGAGGTGAGCTTAGAGAAATGCCTCCTCACGTACGGAGTCTGAAGCCACTGTCAAGCGATCACTCTGAGCAGGAGCCAGATAAACTGTCTGCATATTTAAGGGCCGAGGACAGGAAGATTATGTCAAACAATATGCTTAGTATTGCCAAGAAGCAACTGAAGACCCAGTGTACTAATAGCAATTCTCAGTTACTTGTAAGGGAAATGTCAAAGGGAAAAACAATCAGTAAATTTCCAGAACCTTACGGTGGCTTTGGTAGTGAGGTTGTAGGCCAGAATAATAGAAAACTCCGTTATGGTTCTGAGGTAGCTAGTGATGCAGAGTTGAAAGCAAATGTCAACAATGATCGCCTTTCTTCACATGTACAAAAGCAGTCTGGCACAAAATCTATCAGTTACGGGATCAGCTTGAGGGAGTGGCTTAAACCCGGGAGTCACAAAGTAGATATAGCTGAAAGCCTGCTCATATTTAGGCAGATCGTGGAGTTGGTTGATTTTGAACACTCTCAGGGTGTTGCCTTGCAAGACTTACGACCATCGTGCTTCATTTTGTTGCCATCAAATAATATTAAATACACAGGTGCATCAGCCATCGAAGAACCAGTAAGTATTGTGAACCGAGATTTGATTAACAAAAGGAAATTCGAGCAGGATGCACATGCGGAACGTATTTTAGGTGGAAAACAAATAAAAATGAGTGAAGGTAACGAGGATGAATATTTCACAGCTGGTCCAAAAGAATTTGGGTTTGGTGAACTCCAGTTTCAAATGAACTCATGCTACCTAAACAAATTGGTTGCAGCACAGCAAGGATCAACTTCTGTAATTGTTCAGTTGGAAGAGAAGTGGTATACCAGTCCGGAGGAGTTCAATAAGAGAGGTTGCACACTTTCATCAAATGTATATTCCCTTGGGATCCTTCTTTTTGAGTTGTTGTATCGCTGTAACTCGTGGGAGGTGCTTTCTGCAGTGATGTGGGATTTGCACGATCGGATTCTGCCACCAAAGTTTCTTTCTCAAAATCCATCGGGAGCTGGCTTTTGCTTTTGGCTTCTCCATCCTGAACCTTTGTCTCGTCCAACAACTAGAGAGATCCTGCAGTCTAAACTGATAGGTGGAAACCAAGAATCGGCTTCTTCTGATGATTTCTCAAAATCTGCTGATAATTTTGATGCGGAATCAGAGCCATTACTTAGTTTTCTCAGCTCATTAAAAGACAAGAAGCAAGGTCATGCCTCTAAGTTAGTTGAAGATTTAAGAGGCCTAGAAGAAGTTATAAACAAGTGTGGGAGGAGGCACTTATCAGGGATATCCTCATATGTTTCCTTGTCACACGAGGAATTCCATAGCGACAGAGAACAGGGTTCAGCGTCAAACTTGATTGAGATGCAATTGATGAAGAATATCAACCAGTTAGGGGATGCTTACGCTTCCACAAGATCCCAGATGGGGCTAACGGCAACTGCACCTGCAGCACGCTCTGATAAGGAGTTGTTAAACAATCGAGGCAGTTGGCGTCATGTGGGAAATGATAGTCAGGATTCAAGCACCAACGAGAAATCAGGTGATCGGCTTGGAGCCTTTTCTGATGGTTTAGGCAAGTTTTCTCGCTGTAGCAAGTTTGAAGTATGTGGGACATTAAGAAATCAAGATCTTCTAAACTCTAGCAATGTGATTTGCTGTTTGAGTTTTGACCGTGATGAGGAGTACATTGCTACGGCTGGAGTATCAAAGAAAATCAAGATCTTTGATTTTGACGCGCTTGCAGACAATTCTTTGGATATCCATTATCCTGTAGTTGAAATGCCACACAAATCTAAGCTAAGCTGCATTTGCTGGAACAACTATTTTAAGAACCATCTGGCATCATCGGATTATGATGGAGTCGTACAGATGTGGGATGCAACTACTGGTAAAGGATTTTCTCAGTATGTAGAGCACCAAGGGAGGGCTTGGTGTGTCGACTTCTCTCAAGCTGACCCAAAGAAGTTCGCTAGTGGAAGTGATGACTGTACAAAATATTCAATAGACACAATCTGGAACGCTGCTAATGTTTGTTGTGTTCAGTTCTCTGCTTACTCTTCCAATCTTGTGGTTTTTGGATCCGCTGATTATAAAATTTATGGCTATGATCTTCGAAATACTAGAATCCCTTGGTGCACCTTACCTGGTCATGGAAAATCTGTTAGCTATGTAAAATTTGTAGATGCGCAAACCCTTGTTTCTGCATCTACAGACAACACTCTGAAGCCTTGGGATCTAAACCAATCTATCTCAACTGGGTTGTCTCCTAATGCCTGCAGCTTAACATTCAGTGGTCATACTAATGAGAAGAATTTCGTGGGTTTATCTGCTTCGGATGGATACATAGCATGTGGATCAGAAACAAACGAGGTTTATAATTATTACAGAAGTCTACCGATGCCCATCACTTCACACAAATTTGGATCAATCGACCCTGTTTCTGGAAGTGAGGTTGGTGATTACAGCGGGCAGTTTGTCTCGAGTGTCTGTTGGAGAAGAAAGTGTAACATAGTTGTTGCTGCCAACTCAAGTGGAACTTTGAAATTATTGCAGATGGTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000151 GO:0003006 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006996 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009637 GO:0009639 GO:0009640 GO:0009648 GO:0009657 GO:0009658 GO:0009791 GO:0009987 GO:0010017 GO:0010099 GO:0010100 GO:0010114 GO:0010218 GO:0010228 GO:0016043 GO:0016604 GO:0022414 GO:0023052 GO:0031461 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032991 GO:0042802 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044451 GO:0044464 GO:0048519 GO:0048572 GO:0048573 GO:0048575 GO:0048580 GO:0048581 GO:0048583 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051093 GO:0051239 GO:0051241 GO:0051716 GO:0061458 GO:0065007 GO:0070013 GO:0071214 GO:0071478 GO:0071482 GO:0071489 GO:0071840 GO:0080008 GO:0104004 GO:1902494 GO:1990234 GO:2000026 GO:2000028 GO:2000030 GO:2000241

KEGG Pathways

Metabolic & Signaling

ko04712 map04712

Pfam Domains

Protein Families

Pkinase WD40

Protein Analysis

993

Amino Acids

110.1

Weight (kDa)

6.48

Isoelectric Point (pI)

42.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	408 - 501	5.9e-06	Protein kinase domain
Beta-prop_TEP1_2nd	PF25047	685 - 780	1.5e-06	TEP-1 second beta-propeller
Beta-prop_WDR5	PF25175	687 - 933	1.1e-19	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	689 - 801	1.9e-08	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	694 - 793	2.8e-09	RIG second beta-propeller
Beta-prop_THOC3	PF25174	732 - 821	1.2e-07	THOC3 beta-propeller domain
Beta-prop_THOC3	PF25174	814 - 990	1.3e-06	THOC3 beta-propeller domain
WD40_Prp19	PF24814	814 - 926	1.6e-09	Prp19 WD40 domain
Beta-prop_WDR3_1st	PF25173	815 - 931	2.1e-10	WDR3 first beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0007686)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46340 AT2G46340
fragaria_vesca	FvH4_7g15970
malus_domestica	MD01G1076400.v1.1 MD07G1145300.v1.1
prunus_persica	Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1 Prupe.2G183800_v2.0.a1
pyrus_communis	pycom01g10530
rosa_chinensis	RchiOBHm_Chr1g0358871
rosa_laevigata	RLG00000027920
rosa_multiflora	Rmu_sc0008757.1_g000013
rosa_roxburghii	Rroxscaffold_4G00297550
rosa_rugosa	Rorug01G0265700 Rorug01G0265800 Rorug01G0265900 Rorug01G0266000
rosa_samantha	Rh1AG279700 Rh1BG246600 Rh1CG263700 Rh1DG275000
rosa_wichuraiana	Rw1G024850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	1670, 2508, 2793
AarI	CACCTGC	1 cut(s)	1870
Acc16I	TGCGCA	1 cut(s)	2604
Acc36I	ACCTGC	1 cut(s)	1870
AccI	GTMKAC	4 cut(s)	356, 1244, 2355, 2809
AccII	CGCG	1 cut(s)	2153
AccIII	TCCGGA	1 cut(s)	1252
AciI	CCGC	4 cut(s)	1055, 1568, 2499, 2883
AcuI	CTGAAG	3 cut(s)	455, 572, 2660
AcyI	GRCGYC	1 cut(s)	1909
AfaI	GTAC	7 cut(s)	304, 426, 565, 759, 2102, 2283, 2407
AflIII	ACRYGT	1 cut(s)	754
AjnI	CCWGG	1 cut(s)	2560
AjuI	GAANNNNNNNTTGG	4 cut(s)	1125, 1157, 1390, 1422
Alw21I	GWGCWC	2 cut(s)	2334, 2555
Alw26I	GTCTC	3 cut(s)	1470, 2685, 2899
Alw44I	GTGCAC	1 cut(s)	2551
Ama87I	CYCGRG	2 cut(s)	821, 2896
Aor13HI	TCCGGA	1 cut(s)	1252
AoxI	GGCC	4 cut(s)	200, 495, 664, 1654
ApaLI	GTGCAC	1 cut(s)	2551
ApeKI	GCWGC	6 cut(s)	1193, 1865, 2220, 2438, 2694, 2936
Asp700I	GAANNNNTTC	1 cut(s)	2729
AspLEI	GCGC	2 cut(s)	2155, 2605
AspS9I	GGNCC	2 cut(s)	495, 1124
AsuC2I	CCSGG	2 cut(s)	822, 823
AsuHPI	GGTGA	4 cut(s)	410, 1157, 1970, 2885
AsuII	TTCGAA	1 cut(s)	2530
AvaI	CYCGRG	2 cut(s)	821, 2896
AvaII	GGWCC	1 cut(s)	1124
BaeGI	GKGCMC	1 cut(s)	2555
BamHI	GGATCC	2 cut(s)	1308, 2495
BauI	CACGAG	3 cut(s)	321, 1342, 1727
BbsI	GAAGAC	1 cut(s)	560
Bbv12I	GWGCWC	2 cut(s)	2334, 2555
BbvI	GCAGC	6 cut(s)	1205, 1877, 2207, 2425, 2706, 2923
BceAI	ACGGC	3 cut(s)	231, 1866, 2127
BcgI	CGANNNNNNTGC	4 cut(s)	915, 949, 1028, 1062
BciT130I	CCWGG	1 cut(s)	2562
BciVI	GTATCC	1 cut(s)	2750
BclI	TGATCA	1 cut(s)	373
BcnI	CCSGG	2 cut(s)	822, 823
BcoDI	GTCTC	3 cut(s)	1470, 2685, 2899
BfaI	CTAG	7 cut(s)	282, 705, 1479, 1658, 2058, 2388, 2538
BfmI	CTRYAG	7 cut(s)	1359, 1490, 1863, 2005, 2187, 2625, 2692
BfuAI	ACCTGC	1 cut(s)	1870
BfuI	GTATCC	1 cut(s)	2750
BglII	AGATCT	2 cut(s)	2044, 2136
BisI	GCNGC	6 cut(s)	1194, 1866, 2221, 2439, 2695, 2937
BlpI	GCTNAGC	1 cut(s)	2216
BlsI	GCNGC	6 cut(s)	1195, 1867, 2222, 2440, 2696, 2938
Bme1390I	CCNGG	3 cut(s)	822, 823, 2562
Bme18I	GGWCC	1 cut(s)	1124
BmeT110I	CYCGRG	2 cut(s)	821, 2896
BmgT120I	GGNCC	2 cut(s)	495, 1124
BmiI	GGNNCC	4 cut(s)	468, 1310, 1972, 2497
BmrFI	CCNGG	3 cut(s)	822, 823, 2562
BmrI	ACTGGG	2 cut(s)	553, 2682
BmuI	ACTGGG	2 cut(s)	553, 2682
BpiI	GAAGAC	1 cut(s)	560
BpmI	CTGGAG	4 cut(s)	122, 164, 1136, 2136
Bpu1102I	GCTNAGC	1 cut(s)	2216
Bpu14I	TTCGAA	1 cut(s)	2530
BpuEI	CTTGAG	4 cut(s)	53, 823, 2349, 2929
BpuMI	CCSGG	2 cut(s)	822, 823
BsaAI	YACGTR	1 cut(s)	424
BsaBI	GATNNNNATC	2 cut(s)	84, 2819
BsaHI	GRCGYC	1 cut(s)	1909
BsaJI	CCNNGG	7 cut(s)	197, 498, 821, 1303, 2335, 2546, 2645
BsaWI	WCCGGW	1 cut(s)	1252
Bse1I	ACTGG	9 cut(s)	105, 147, 559, 995, 1153, 1248, 1804, 2308, 2677
Bse3DI	GCAATG	2 cut(s)	2068, 2103
Bse8I	GATNNNNATC	2 cut(s)	84, 2819
BseAI	TCCGGA	1 cut(s)	1252
BseBI	CCWGG	1 cut(s)	2562
BseDI	CCNNGG	7 cut(s)	197, 498, 821, 1303, 2335, 2546, 2645
BseGI	GGATG	8 cut(s)	10, 85, 1051, 1111, 1450, 1819, 2299, 2758
BseJI	GATNNNNATC	2 cut(s)	84, 2819
BseMI	GCAATG	2 cut(s)	2068, 2103
BseMII	CTCAG	7 cut(s)	279, 450, 593, 687, 914, 1609, 2333
BseNI	ACTGG	9 cut(s)	105, 147, 559, 995, 1153, 1248, 1804, 2308, 2677
BseRI	GAGGAG	4 cut(s)	408, 1271, 1699, 2111
BseSI	GKGCMC	1 cut(s)	2555
BseXI	GCAGC	6 cut(s)	1205, 1877, 2207, 2425, 2706, 2923
BsgI	GTGCAG	1 cut(s)	1842
Bsh1236I	CGCG	1 cut(s)	2153
Bsh1285I	CGRYCG	1 cut(s)	1384
BshFI	GGCC	4 cut(s)	202, 497, 666, 1656
BsiEI	CGRYCG	1 cut(s)	1384
BsiHKAI	GWGCWC	2 cut(s)	2334, 2555
BsiHKCI	CYCGRG	2 cut(s)	821, 2896
BsiSI	CCGG	3 cut(s)	311, 822, 1253
BsiWI	CGTACG	1 cut(s)	424
BslFI	GGGAC	1 cut(s)	2041
BsmAI	GTCTC	3 cut(s)	1470, 2685, 2899
BsmFI	GGGAC	1 cut(s)	2041
BsnI	GGCC	4 cut(s)	202, 497, 666, 1656
BsoBI	CYCGRG	2 cut(s)	821, 2896
Bsp119I	TTCGAA	1 cut(s)	2530
Bsp1286I	GDGCHC	2 cut(s)	2334, 2555
Bsp13I	TCCGGA	1 cut(s)	1252
Bsp1407I	TGTACA	2 cut(s)	757, 2405
Bsp1720I	GCTNAGC	1 cut(s)	2216
BspACI	CCGC	4 cut(s)	1055, 1568, 2499, 2883
BspANI	GGCC	4 cut(s)	202, 497, 666, 1656
BspCNI	CTCAG	7 cut(s)	278, 451, 592, 688, 913, 1608, 2332
BspEI	TCCGGA	1 cut(s)	1252
BspFNI	CGCG	1 cut(s)	2153
BspLI	GGNNCC	4 cut(s)	468, 1310, 1972, 2497
BspMAI	CTGCAG	4 cut(s)	1363, 1494, 1867, 2696
BspMI	ACCTGC	1 cut(s)	1870
BspT104I	TTCGAA	1 cut(s)	2530
BsrDI	GCAATG	2 cut(s)	2068, 2103
BsrGI	TGTACA	2 cut(s)	757, 2405
BsrI	ACTGG	9 cut(s)	105, 147, 559, 995, 1153, 1248, 1804, 2308, 2677
BssECI	CCNNGG	7 cut(s)	197, 498, 821, 1303, 2335, 2546, 2645
BssNAI	GTATAC	1 cut(s)	1245
BssNI	GRCGYC	1 cut(s)	1909
BssSI	CACGAG	3 cut(s)	321, 1342, 1727
BssT1I	CCWWGG	4 cut(s)	1303, 2335, 2546, 2645
Bst1107I	GTATAC	1 cut(s)	1245
Bst2BI	CACGAG	3 cut(s)	321, 1342, 1727
Bst2UI	CCWGG	1 cut(s)	2562
Bst4CI	ACNGT	5 cut(s)	444, 480, 641, 2090, 2405
Bst6I	CTCTTC	2 cut(s)	1227, 2479
BstACI	GRCGYC	1 cut(s)	1909
BstAUI	TGTACA	2 cut(s)	757, 2405
BstBAI	YACGTR	1 cut(s)	424
BstBI	TTCGAA	1 cut(s)	2530
BstC8I	GCNNGC	7 cut(s)	204, 854, 1429, 1870, 2157, 2692, 2885
BstDSI	CCRYGG	1 cut(s)	197
BstF5I	GGATG	8 cut(s)	10, 85, 1051, 1111, 1450, 1819, 2299, 2758
BstFNI	CGCG	1 cut(s)	2153
BstHHI	GCGC	2 cut(s)	2155, 2605
BstMAI	GTCTC	3 cut(s)	1470, 2685, 2899
BstMCI	CGRYCG	1 cut(s)	1384
BstMWI	GCNNNNNNNGC	3 cut(s)	710, 1865, 2226
BstNI	CCWGG	1 cut(s)	2562
BstNSI	RCATGY	3 cut(s)	758, 1055, 2769
BstSCI	CCNGG	3 cut(s)	820, 821, 2560
BstSFI	CTRYAG	7 cut(s)	1359, 1490, 1863, 2005, 2187, 2625, 2692
BstSLI	GKGCMC	1 cut(s)	2555
BstUI	CGCG	1 cut(s)	2153
BstV1I	GCAGC	6 cut(s)	1205, 1877, 2207, 2425, 2706, 2923
BstV2I	GAAGAC	1 cut(s)	560
BstX2I	RGATCY	8 cut(s)	307, 1308, 1485, 1832, 2044, 2136, 2495, 2650
BstXI	CCANNNNNNTGG	1 cut(s)	2485
BstYI	RGATCY	8 cut(s)	307, 1308, 1485, 1832, 2044, 2136, 2495, 2650
BstZ17I	GTATAC	1 cut(s)	1245
BsuI	GTATCC	1 cut(s)	2750
BsuRI	GGCC	4 cut(s)	202, 497, 666, 1656
BtgI	CCRYGG	1 cut(s)	197
BtsCI	GGATG	8 cut(s)	10, 85, 1051, 1111, 1450, 1819, 2299, 2758
BtsI	GCAGTG	1 cut(s)	1368
BtsIMutI	CAGTG	5 cut(s)	140, 440, 566, 1368, 2713
BveI	ACCTGC	1 cut(s)	1870
Cac8I	GCNNGC	7 cut(s)	204, 854, 1429, 1870, 2157, 2692, 2885
CfoI	GCGC	2 cut(s)	2155, 2605
Cfr13I	GGNCC	2 cut(s)	495, 1124
Cfr9I	CCCGGG	1 cut(s)	821
CseI	GACGC	3 cut(s)	1752, 1898, 2159
CsiI	ACCWGGT	1 cut(s)	2560
Csp6I	GTAC	7 cut(s)	303, 425, 564, 758, 2101, 2282, 2406
CspCI	CAANNNNNGTGG	2 cut(s)	2929, 2964
CviAII	CATG	7 cut(s)	755, 1052, 1170, 1625, 1913, 2567, 2766
CviQI	GTAC	7 cut(s)	303, 425, 564, 758, 2101, 2282, 2406
DraI	TTTAAA	1 cut(s)	190
Eam1104I	CTCTTC	2 cut(s)	1227, 2479
EarI	CTCTTC	2 cut(s)	1227, 2479
Eco130I	CCWWGG	4 cut(s)	1303, 2335, 2546, 2645
Eco147I	AGGCCT	1 cut(s)	1656
Eco32I	GATATC	2 cut(s)	1704, 2176
Eco47I	GGWCC	1 cut(s)	1124
Eco57I	CTGAAG	3 cut(s)	455, 572, 2660
Eco88I	CYCGRG	2 cut(s)	821, 2896
EcoRI	GAATTC	1 cut(s)	1733
EcoRII	CCWGG	1 cut(s)	2560
EcoRV	GATATC	2 cut(s)	1704, 2176
EcoT14I	CCWWGG	4 cut(s)	1303, 2335, 2546, 2645
ErhI	CCWWGG	4 cut(s)	1303, 2335, 2546, 2645
FaeI	CATG	7 cut(s)	758, 1055, 1173, 1628, 1916, 2570, 2769
FalI	AAGNNNNNCTT	2 cut(s)	2938, 2970
FaqI	GGGAC	1 cut(s)	2041
FatI	CATG	7 cut(s)	754, 1051, 1169, 1624, 1912, 2566, 2765
FauI	CCCGC	1 cut(s)	2876
FauNDI	CATATG	1 cut(s)	1711
FbaI	TGATCA	1 cut(s)	373
FblI	GTMKAC	4 cut(s)	356, 1244, 2355, 2809
Fnu4HI	GCNGC	6 cut(s)	1194, 1866, 2221, 2439, 2695, 2937
FokI	GGATG	8 cut(s)	17, 92, 1058, 1118, 1437, 1826, 2306, 2765
Fsp4HI	GCNGC	6 cut(s)	1194, 1866, 2221, 2439, 2695, 2937
FspBI	CTAG	7 cut(s)	282, 705, 1479, 1658, 2058, 2388, 2538
FspI	TGCGCA	1 cut(s)	2604
GlaI	GCGC	2 cut(s)	2154, 2604
GluI	GCNGC	6 cut(s)	1194, 1866, 2221, 2439, 2695, 2937
GsuI	CTGGAG	4 cut(s)	122, 164, 1136, 2136
HaeIII	GGCC	4 cut(s)	202, 497, 666, 1656
HapII	CCGG	3 cut(s)	311, 822, 1253
HgaI	GACGC	3 cut(s)	1752, 1898, 2159
HhaI	GCGC	2 cut(s)	2155, 2605
Hin1I	GRCGYC	1 cut(s)	1909
Hin1II	CATG	7 cut(s)	758, 1055, 1173, 1628, 1916, 2570, 2769
Hin6I	GCGC	2 cut(s)	2153, 2603
HinP1I	GCGC	2 cut(s)	2153, 2603
HincII	GTYRAC	2 cut(s)	733, 2356
HindII	GTYRAC	2 cut(s)	733, 2356
HinfI	GANTC	9 cut(s)	263, 430, 826, 1387, 1520, 1571, 1934, 2277, 2541
HpaII	CCGG	3 cut(s)	311, 822, 1253
HphI	GGTGA	4 cut(s)	410, 1157, 1970, 2885
HpyAV	CCTTC	2 cut(s)	1088, 1322
HpyCH4III	ACNGT	5 cut(s)	444, 480, 641, 2090, 2405
HpyCH4IV	ACGT	2 cut(s)	423, 1060
HpyF10VI	GCNNNNNNNGC	3 cut(s)	710, 1865, 2226
HpySE526I	ACGT	2 cut(s)	423, 1060
Hsp92I	GRCGYC	1 cut(s)	1909
Hsp92II	CATG	7 cut(s)	758, 1055, 1173, 1628, 1916, 2570, 2769
HspAI	GCGC	2 cut(s)	2153, 2603
Kpn2I	TCCGGA	1 cut(s)	1252
Ksp22I	TGATCA	1 cut(s)	373
LmnI	GCTCC	3 cut(s)	466, 1424, 1970
Lsp1109I	GCAGC	6 cut(s)	1205, 1877, 2207, 2425, 2706, 2923
MabI	ACCWGGT	1 cut(s)	2560
MaeI	CTAG	7 cut(s)	282, 705, 1479, 1658, 2058, 2388, 2538
MaeII	ACGT	2 cut(s)	423, 1060
MaeIII	GTNAC	8 cut(s)	169, 582, 788, 827, 1097, 1337, 1722, 2921
MfeI	CAATTG	1 cut(s)	1784
MflI	RGATCY	8 cut(s)	307, 1308, 1485, 1832, 2044, 2136, 2495, 2650
MhlI	GDGCHC	2 cut(s)	2334, 2555
MlyI	GAGTC	4 cut(s)	257, 439, 835, 2286
MmeI	TCCRAC	4 cut(s)	99, 1209, 1496, 2888
MroI	TCCGGA	1 cut(s)	1252
MroXI	GAANNNNTTC	1 cut(s)	2729
MslI	CAYNNNNRTG	1 cut(s)	2718
MspA1I	CMGCKG	3 cut(s)	1121, 2501, 2883
MspI	CCGG	3 cut(s)	311, 822, 1253
MspR9I	CCNGG	3 cut(s)	822, 823, 2562
MssI	GTTTAAAC	1 cut(s)	190
MunI	CAATTG	1 cut(s)	1784
MvaI	CCWGG	1 cut(s)	2562
MvnI	CGCG	1 cut(s)	2153
MwoI	GCNNNNNNNGC	3 cut(s)	710, 1865, 2226
NciI	CCSGG	2 cut(s)	822, 823
NdeI	CATATG	1 cut(s)	1711
NlaIII	CATG	7 cut(s)	758, 1055, 1173, 1628, 1916, 2570, 2769
NlaIV	GGNNCC	4 cut(s)	468, 1310, 1972, 2497
NmeAIII	GCCGAG	1 cut(s)	523
NmuCI	GTSAC	2 cut(s)	827, 1722
NsbI	TGCGCA	1 cut(s)	2604
NspI	RCATGY	3 cut(s)	758, 1055, 2769
NspV	TTCGAA	1 cut(s)	2530
PaeR7I	CTCGAG	1 cut(s)	2896
PaqCI	CACCTGC	1 cut(s)	1870
PceI	AGGCCT	1 cut(s)	1656
PciI	ACATGT	1 cut(s)	754
PdmI	GAANNNNTTC	1 cut(s)	2729
PfeI	GAWTC	5 cut(s)	1387, 1520, 1571, 1934, 2541
Pfl23II	CGTACG	1 cut(s)	424
PkrI	GCNGC	6 cut(s)	1195, 1867, 2222, 2440, 2696, 2938
Ple19I	CGATCG	1 cut(s)	1384
PleI	GAGTC	4 cut(s)	257, 438, 834, 2285
PmeI	GTTTAAAC	1 cut(s)	190
PpsI	GAGTC	4 cut(s)	257, 438, 834, 2285
Ppu21I	YACGTR	1 cut(s)	424
PscI	ACATGT	1 cut(s)	754
PsiI	TTATAA	3 cut(s)	1670, 2508, 2793
Psp6I	CCWGG	1 cut(s)	2560
PspGI	CCWGG	1 cut(s)	2560
PspLI	CGTACG	1 cut(s)	424
PspN4I	GGNNCC	4 cut(s)	468, 1310, 1972, 2497
PspPI	GGNCC	2 cut(s)	495, 1124
PsrI	GAACNNNNNNTAC	2 cut(s)	1157, 1189
PstI	CTGCAG	4 cut(s)	1363, 1494, 1867, 2696
PsuI	RGATCY	8 cut(s)	307, 1308, 1485, 1832, 2044, 2136, 2495, 2650
PvuI	CGATCG	1 cut(s)	1384
PvuII	CAGCTG	1 cut(s)	1121
RsaI	GTAC	7 cut(s)	304, 426, 565, 759, 2102, 2283, 2407
RsaNI	GTAC	7 cut(s)	303, 425, 564, 758, 2101, 2282, 2406
RseI	CAYNNNNRTG	1 cut(s)	2718
SalI	GTCGAC	1 cut(s)	2354
SatI	GCNGC	6 cut(s)	1194, 1866, 2221, 2439, 2695, 2937
Sau96I	GGNCC	2 cut(s)	495, 1124
SchI	GAGTC	4 cut(s)	257, 439, 835, 2286
ScrFI	CCNGG	3 cut(s)	822, 823, 2562
SduI	GDGCHC	2 cut(s)	2334, 2555
SexAI	ACCWGGT	1 cut(s)	2560
SfcI	CTRYAG	7 cut(s)	1359, 1490, 1863, 2005, 2187, 2625, 2692
Sfr274I	CTCGAG	1 cut(s)	2896
SfuI	TTCGAA	1 cut(s)	2530
SinI	GGWCC	1 cut(s)	1124
SlaI	CTCGAG	1 cut(s)	2896
SmaI	CCCGGG	1 cut(s)	823
SmiMI	CAYNNNNRTG	1 cut(s)	2718
SmlI	CTYRAG	5 cut(s)	68, 802, 2364, 2896, 2944
SmoI	CTYRAG	5 cut(s)	68, 802, 2364, 2896, 2944
SseBI	AGGCCT	1 cut(s)	1656
SsiI	CCGC	4 cut(s)	1055, 1568, 2499, 2883
SspI	AATATT	3 cut(s)	961, 1112, 2414
SspMI	CTAG	7 cut(s)	282, 705, 1479, 1658, 2058, 2388, 2538
StuI	AGGCCT	1 cut(s)	1656
StyD4I	CCNGG	3 cut(s)	820, 821, 2560
StyI	CCWWGG	4 cut(s)	1303, 2335, 2546, 2645
TaaI	ACNGT	5 cut(s)	444, 480, 641, 2090, 2405
TaiI	ACGT	2 cut(s)	426, 1063
TaqI	TCGA	7 cut(s)	987, 1038, 1897, 2355, 2530, 2847, 2897
TatI	WGTACW	4 cut(s)	563, 757, 2100, 2405
TfiI	GAWTC	5 cut(s)	1387, 1520, 1571, 1934, 2541
TscAI	CASTG	5 cut(s)	147, 447, 566, 1368, 2713
TseFI	GTSAC	2 cut(s)	827, 1722
TseI	GCWGC	6 cut(s)	1193, 1865, 2220, 2438, 2694, 2936
Tsp45I	GTSAC	2 cut(s)	827, 1722
TspDTI	ATGAA	7 cut(s)	75, 168, 928, 1122, 1178, 1275, 1805
TspGWI	ACGGA	2 cut(s)	442, 674
TspMI	CCCGGG	1 cut(s)	821
TspRI	CASTG	5 cut(s)	147, 447, 566, 1368, 2713
VneI	GTGCAC	1 cut(s)	2551
VpaK11BI	GGWCC	1 cut(s)	1124
XceI	RCATGY	3 cut(s)	758, 1055, 2769
XhoI	CTCGAG	1 cut(s)	2896
XmaI	CCCGGG	1 cut(s)	821
XmiI	GTMKAC	4 cut(s)	356, 1244, 2355, 2809
XmnI	GAANNNNTTC	1 cut(s)	2729
XspI	CTAG	7 cut(s)	282, 705, 1479, 1658, 2058, 2388, 2538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.