Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0007002

Physical Location & Seq

Genomic Coordinates Forward (+)

18351816 .. 18356762

Gene Structure 4947 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1076900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2754 bp

ATGGGGGACTCGGCTGCTACAGTGAGGCTGGTTCGGTGCCCCAAGTGTGAGAATCTTCTTCCGGAGCTTGATGATTACTCTGTTTACCAGTGTGGTGGTTGTGGTGCTGTTCTTCGAGCAAATAAAAAGAGGCAAGAGGGGGATACTTTGTTGGAGAAGTCAGACAAAGAGAGGGTTGGAGGAGTTTGTGCCAAATCAGATGATTCAGACAGTAAAGGAATACTTGTGTTGAGTGATACTTCTGATATGGATGTTAAGTCGGCTGATGCTTCTTTGAGGTGTGATCAAAGGGATTTAGAGAAGGGAAATGTGAAAAATGCTGAGGTCGTTAGGAAGCAAGCGAAAGATGCAAGTCAGAACTGGTTTGTCGAGGCTGGCGTTGATATGAGCATGAAAAGAGATGAATTGGAGAATGCTTCGGGAAGAGAACATGGAGATTTGAATGCCCAATTTGGTCCCATAAGTGCAAGTCGAAGATCGCAATGGATGGCAGATTGGCGAGCTCAAGAGAATGGTGAAAAGGAGGGAGTTCGAAGACATCAAAGAACTGATTTTGGAGGTATGAGGTCTTCAACTTCAACCTATCAAGACGAGGGCCCGTCAAATTACCATTTGGGTTCTTCGTATAGGGGTGGGGAACCATTGAGAAAAAGTATTGTCCCAGATGAGGCAAACAGAGTTCTGTACCTTGAACAAGACCGAGCCGAGCTTCTGAAGAAGCTGGATGAGCTGAGGGATCAACTTAGCCGGTCTTGTGATTTGGTTGATAAGCCAAAAGAGAAAGCTCCTGTTGATGGTGGGATGGTTCCTCCAGATCCTTATGGTAGTTCTGACACTTCATATGCAGGTGTTTCTTCCGCGGCAAATAGGGCTTCGATGCAGTACTTTGGACCTGGTAAACCTGTCGCAGCACATGCTCATTTCAATCACTTTCCAGAGGCATTTCCCTATACCAATGGCCATGAAATGCCTATGGCTAGCTTTTCTTCTACCATGCATAATTCAAACAATTTTCCTACATATGGCGATCCTTTTGGATCCCACATGCACAGTGGAGCTCCTCATCCCTTTCCCAGGCAATACCAGCAACCATCGCTTCCATACTTCTCTGGACAGTATGCTGAAAACAATCCTGATCCATTTGAGCTTTACCCAAATAATGCATCGGTTCACCATCCTACTTGCCCTTGCTTTTATTGCTATGACAATCACCGGCGGGGTTCAGTGCCAGTTCCACCCACTGCTTTCCACAATAAAAGGTTCCCTGATTTCCCTAGTAGTTCCATGTTCCATCCTGGAAACCCGGGGATGGTCGGTCCATATGATCACAACAAACCTCGGACTTCGGTTCCTCCACCCTTTAATGTTTCGCAAACCCATACAAGATGGCCAAGTGACCTTAACCCCAAAATGGGAAGCTTTGTTCATCCCCGTCCAGAGAGGGTGGTGCCAACCAGTGGTGGCCGACGATGTCTTCCATTTTCTGGTGGTGCTCCATTTGTAACATGTAATAATTGCTTTGAAATTCTGCAGCTGCCCAAAAAAGTATTGCTTGCCGAAAAGAATAAACAGAAAGTGCGATGCGGGGCATGCTTTACTGTGATTGATTTTGCTGTTTCTAATAAGAAACTAGTTCTTTCTCATGCAGAAGCAAAGAAAAATCCGTCGGAAGTTAATAGCTTGAATGAGGTGGTCAATGACAGTACTTTACATTCACAAGGCCATGTCACCCGGATCTATGCTCATTTCTCCTCTGATGATTATGATACTTCTGGCTATGATTTTCAGTCAATAGATAGAGAACCTGCGTTACCATCCACAGGCCCAAGTTCAACTGGTAGCAAGCCTCACGAGATGCAAAGCTTTCATTCTTCATCTCCCAGTACCTCTGAGGATGACGGCATCCCAGAAGCTACAGTTGCTCCAAAAGAATTCACAAATTCTATTCAGCAGCCAATCAAAGGCACTTTTTCTCCGCCTCCTGCTGGTTCACCTCTAGAGGAACACTTTGAATTCTCATCGAACAATCACGTGATAAACCGACTTGGGAAGGGAAACCGCAGTAGTCGCTCAGATCAAGAGAAGATAAAGCCAAATAAGGTCACCTCGCGACAAAACTCTTTACAGGAGACATCACTAGCAACTGAGATGGAGGTATCATTTAATGAGTACTCCAACACTGGGGTTTCCCAAGATTCTTGGGATGCAAACAAAGAGGAGCATCAGCCAAGAACCAACAAAGGGAGTGATTCATTTATTACGAACTTTATTAAGAAGAGCTTCAGGGATTTTTCCAAATCAAATCAGACAAGTGATGGGAGAAGTAATGTTTCGGTTAATGGGAGTTTAATACCAGATCGTGTGCTCAAGAAGGCCGAAAAGATGGCTGGAACAGTCCATCCTGGACAATATTGGTATGATTTCCGAGCTGGATTCTGGGGTGTCATGGGTGGCCCTGGTCTTGGCATCATTCCTCCATTTATTGAAGAGTTCAACTATCCCATGCCTCAGAACTGTGCGGCCGGAGATACCAGTATTTTTGTGAATGGAAGGGAGCTTCACCATAAAGATTTGGATTTGCTGTCCAGTAGAGGACTTCCCACAACCAGAGATAGATCTTATATCATCGAGATCTCAGGGAGAGTCCTGGATGAGGACACTGGTGAAGAGCTTGATAGCCTTGGCAAGCTAGCCCCAACAGTTGAGAAGGTAAAACGTGGATTTGGCATGAAGCTTCCAAGAGCAGCTGCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zinc_ribbon_12

Protein Analysis

918

Amino Acids

101.36

Weight (kDa)

6.41

Isoelectric Point (pI)

48.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EDR4-like_1st	PF22910	8 - 40	4.2e-20	Enhanced disease resistance 4-like, N-terminal domain
Zn_ribbon_12	PF11331	496 - 539	5.7e-21	EDR4 zinc-ribbon domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007002)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46380 AT2G46380 AT3G61670 AT3G61670
fragaria_vesca	FvH4_7g16060 FvH4_7g16060 FvH4_7g16060 FvH4_7g16060 FvH4_7g16060
malus_domestica	MD01G1076900.v1.1 MD07G1146100.v1.1 MD07G1146300.v1.1
prunus_persica	Prupe.2G184400_v2.0.a1
pyrus_communis	pycom01g10600 pycom07g14570
rosa_chinensis	RchiOBHm_Chr1g0359001
rosa_laevigata	RLG00000027910
rosa_multiflora	Rmu_sc0001176.1_g000013
rosa_roxburghii	Rroxscaffold_4G00297470
rosa_rugosa	Rorug01G0267000
rosa_samantha	Rh1AG280300 Rh1BG247400 Rh1CG264700 Rh1DG275800
rosa_wichuraiana	Rw1G024920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	836
Acc36I	ACCTGC	2 cut(s)	836, 1813
AccB1I	GGYRCC	2 cut(s)	36, 1447
AccB7I	CCANNNNNTGG	2 cut(s)	1457, 1484
AccII	CGCG	2 cut(s)	860, 2110
AccIII	TCCGGA	1 cut(s)	61
AciI	CCGC	7 cut(s)	858, 860, 1216, 1584, 1976, 2059, 2519
AclWI	GGATC	7 cut(s)	744, 809, 1022, 1032, 1045, 1130, 1742
AcoI	YGGCCR	4 cut(s)	958, 1388, 1462, 2520
AcsI	RAATTY	4 cut(s)	1524, 1931, 1939, 2012
AcuI	CTGAAG	2 cut(s)	734, 2266
AcvI	CACGTG	1 cut(s)	2032
AfaI	GTAC	5 cut(s)	686, 884, 1705, 1885, 2171
AflIII	ACRYGT	1 cut(s)	1505
AhlI	ACTAGT	1 cut(s)	1630
AjnI	CCWGG	6 cut(s)	892, 1073, 1294, 2401, 2455, 2646
AloI	GAACNNNNNNTCC	4 cut(s)	1407, 1439, 2383, 2415
Alw21I	GWGCWC	4 cut(s)	505, 1060, 1495, 2367
Alw26I	GTCTC	1 cut(s)	2123
AlwI	GGATC	7 cut(s)	744, 809, 1022, 1032, 1045, 1130, 1742
Ama87I	CYCGRG	1 cut(s)	1303
Aor13HI	TCCGGA	1 cut(s)	61
AoxI	GGCC	9 cut(s)	595, 958, 1388, 1462, 1720, 1822, 2373, 2452, 2520
ApaI	GGGCCC	1 cut(s)	599
ApeKI	GCWGC	7 cut(s)	14, 908, 1531, 1534, 1951, 2744, 2747
ApoI	RAATTY	4 cut(s)	1524, 1931, 1939, 2012
Asp700I	GAANNNNTTC	1 cut(s)	2279
AspS9I	GGNCC	7 cut(s)	455, 595, 596, 890, 1316, 1823, 2453
AsuC2I	CCSGG	3 cut(s)	1304, 1305, 1732
AsuHPI	GGTGA	8 cut(s)	527, 1163, 1202, 1720, 1983, 2095, 2552, 2675
AsuII	TTCGAA	1 cut(s)	532
AsuNHI	GCTAGC	2 cut(s)	977, 2689
AvaI	CYCGRG	1 cut(s)	1303
AvaII	GGWCC	3 cut(s)	455, 890, 1316
BaeGI	GKGCMC	2 cut(s)	41, 599
BalI	TGGCCA	2 cut(s)	960, 1390
BamHI	GGATCC	1 cut(s)	1037
BanI	GGYRCC	2 cut(s)	36, 1447
BanII	GRGCYC	3 cut(s)	505, 599, 1060
BarI	GAAGNNNNNNTAC	2 cut(s)	553, 585
BauI	CACGAG	1 cut(s)	1850
BbrPI	CACGTG	1 cut(s)	2032
BbsI	GAAGAC	3 cut(s)	541, 561, 1466
Bbv12I	GWGCWC	4 cut(s)	505, 1060, 1495, 2367
BbvCI	CCTCAGC	2 cut(s)	321, 731
BbvI	GCAGC	5 cut(s)	920, 1521, 1543, 1963, 2734
BceAI	ACGGC	1 cut(s)	1915
BciT130I	CCWGG	6 cut(s)	894, 1075, 1296, 2403, 2457, 2648
BciVI	GTATCC	1 cut(s)	136
BclI	TGATCA	2 cut(s)	283, 1324
BcnI	CCSGG	3 cut(s)	1304, 1305, 1732
BcoDI	GTCTC	1 cut(s)	2123
BcuI	ACTAGT	1 cut(s)	1630
BfaI	CTAG	6 cut(s)	978, 1275, 1631, 1997, 2138, 2690
BfmI	CTRYAG	3 cut(s)	18, 1529, 1914
BfuAI	ACCTGC	2 cut(s)	836, 1813
BfuI	GTATCC	1 cut(s)	136
BglII	AGATCT	2 cut(s)	2615, 2631
BisI	GCNGC	9 cut(s)	15, 861, 909, 1532, 1535, 1952, 2520, 2745, 2748
BlsI	GCNGC	9 cut(s)	16, 862, 910, 1533, 1536, 1953, 2521, 2746, 2749
BmcAI	AGTACT	3 cut(s)	884, 1705, 2171
Bme1390I	CCNGG	9 cut(s)	894, 1075, 1296, 1304, 1305, 1732, 2403, 2457, 2648
Bme18I	GGWCC	3 cut(s)	455, 890, 1316
BmeT110I	CYCGRG	1 cut(s)	1303
BmgT120I	GGNCC	7 cut(s)	455, 595, 596, 890, 1316, 1823, 2453
BmiI	GGNNCC	9 cut(s)	38, 457, 597, 639, 807, 1039, 1262, 1350, 1449
BmrFI	CCNGG	9 cut(s)	894, 1075, 1296, 1304, 1305, 1732, 2403, 2457, 2648
BmrI	ACTGGG	2 cut(s)	1875, 2190
BmtI	GCTAGC	2 cut(s)	981, 2693
BmuI	ACTGGG	2 cut(s)	1875, 2190
BpiI	GAAGAC	3 cut(s)	541, 561, 1466
BpmI	CTGGAG	1 cut(s)	795
Bpu10I	CCTNAGC	2 cut(s)	321, 731
Bpu14I	TTCGAA	1 cut(s)	532
BpuEI	CTTGAG	2 cut(s)	489, 2351
BpuMI	CCSGG	3 cut(s)	1304, 1305, 1732
BsaAI	YACGTR	1 cut(s)	2032
BsaJI	CCNNGG	7 cut(s)	858, 1073, 1303, 1304, 1337, 2455, 2680
BsaWI	WCCGGW	1 cut(s)	61
BsaXI	ACNNNNNCTCC	4 cut(s)	1906, 1936, 1957, 1987
Bse118I	RCCGGY	2 cut(s)	747, 1212
Bse3DI	GCAATG	1 cut(s)	488
BseAI	TCCGGA	1 cut(s)	61
BseBI	CCWGG	6 cut(s)	894, 1075, 1296, 2403, 2457, 2648
BseDI	CCNNGG	7 cut(s)	858, 1073, 1303, 1304, 1337, 2455, 2680
BseMI	GCAATG	1 cut(s)	488
BseMII	CTCAG	7 cut(s)	312, 722, 1881, 2085, 2136, 2522, 2649
BseRI	GAGGAG	4 cut(s)	195, 1050, 1741, 2231
BseSI	GKGCMC	2 cut(s)	41, 599
BseX3I	CGGCCG	1 cut(s)	2520
BseXI	GCAGC	5 cut(s)	920, 1521, 1543, 1963, 2734
Bsh1236I	CGCG	2 cut(s)	860, 2110
Bsh1285I	CGRYCG	1 cut(s)	2523
BshFI	GGCC	9 cut(s)	597, 960, 1390, 1464, 1722, 1824, 2375, 2454, 2522
BshNI	GGYRCC	2 cut(s)	36, 1447
BsiEI	CGRYCG	1 cut(s)	2523
BsiHKAI	GWGCWC	4 cut(s)	505, 1060, 1495, 2367
BsiHKCI	CYCGRG	1 cut(s)	1303
BsiSI	CCGG	6 cut(s)	62, 748, 1213, 1304, 1732, 2523
BslFI	GGGAC	3 cut(s)	20, 441, 644
BsmAI	GTCTC	1 cut(s)	2123
BsmFI	GGGAC	3 cut(s)	20, 441, 644
BsmI	GAATGC	2 cut(s)	418, 448
BsnI	GGCC	9 cut(s)	597, 960, 1390, 1464, 1722, 1824, 2375, 2454, 2522
BsoBI	CYCGRG	1 cut(s)	1303
Bsp119I	TTCGAA	1 cut(s)	532
Bsp120I	GGGCCC	1 cut(s)	595
Bsp1286I	GDGCHC	6 cut(s)	41, 505, 599, 1060, 1495, 2367
Bsp13I	TCCGGA	1 cut(s)	61
Bsp68I	TCGCGA	1 cut(s)	2110
BspACI	CCGC	7 cut(s)	858, 860, 1216, 1584, 1976, 2059, 2519
BspANI	GGCC	9 cut(s)	597, 960, 1390, 1464, 1722, 1824, 2375, 2454, 2522
BspCNI	CTCAG	7 cut(s)	313, 723, 1882, 2084, 2137, 2521, 2648
BspEI	TCCGGA	1 cut(s)	61
BspFNI	CGCG	2 cut(s)	860, 2110
BspLI	GGNNCC	9 cut(s)	38, 457, 597, 639, 807, 1039, 1262, 1350, 1449
BspMAI	CTGCAG	1 cut(s)	1533
BspMI	ACCTGC	2 cut(s)	836, 1813
BspOI	GCTAGC	2 cut(s)	981, 2693
BspPI	GGATC	7 cut(s)	744, 809, 1022, 1032, 1045, 1130, 1742
BspQI	GCTCTTC	2 cut(s)	2270, 2661
BspT104I	TTCGAA	1 cut(s)	532
BspT107I	GGYRCC	2 cut(s)	36, 1447
BsrDI	GCAATG	1 cut(s)	488
BsrFI	RCCGGY	2 cut(s)	747, 1212
BssAI	RCCGGY	2 cut(s)	747, 1212
BssECI	CCNNGG	7 cut(s)	858, 1073, 1303, 1304, 1337, 2455, 2680
BssSI	CACGAG	1 cut(s)	1850
BssT1I	CCWWGG	1 cut(s)	2680
Bst2BI	CACGAG	1 cut(s)	1850
Bst2UI	CCWGG	6 cut(s)	894, 1075, 1296, 2403, 2457, 2648
Bst6I	CTCTTC	4 cut(s)	418, 2270, 2481, 2661
BstAPI	GCANNNNNTGC	1 cut(s)	914
BstBAI	YACGTR	1 cut(s)	2032
BstBI	TTCGAA	1 cut(s)	532
BstC8I	GCNNGC	9 cut(s)	339, 376, 501, 979, 1554, 1591, 1844, 2687, 2691
BstDEI	CTNAG	8 cut(s)	321, 731, 743, 1890, 2071, 2145, 2508, 2635
BstDSI	CCRYGG	1 cut(s)	858
BstEII	GGTNACC	1 cut(s)	2101
BstENI	CCTNNNNNAGG	2 cut(s)	1072, 2651
BstFNI	CGCG	2 cut(s)	860, 2110
BstMAI	GTCTC	1 cut(s)	2123
BstMCI	CGRYCG	1 cut(s)	2523
BstNI	CCWGG	6 cut(s)	894, 1075, 1296, 2403, 2457, 2648
BstNSI	RCATGY	4 cut(s)	917, 1048, 1509, 1593
BstPI	GGTNACC	1 cut(s)	2101
BstSCI	CCNGG	9 cut(s)	892, 1073, 1294, 1302, 1303, 1730, 2401, 2455, 2646
BstSFI	CTRYAG	3 cut(s)	18, 1529, 1914
BstSLI	GKGCMC	2 cut(s)	41, 599
BstUI	CGCG	2 cut(s)	860, 2110
BstV1I	GCAGC	5 cut(s)	920, 1521, 1543, 1963, 2734
BstV2I	GAAGAC	3 cut(s)	541, 561, 1466
BstX2I	RGATCY	5 cut(s)	814, 1037, 1734, 2615, 2631
BstXI	CCANNNNNNTGG	1 cut(s)	95
BstYI	RGATCY	5 cut(s)	814, 1037, 1734, 2615, 2631
BstZI	CGGCCG	1 cut(s)	2520
BsuI	GTATCC	1 cut(s)	136
BsuRI	GGCC	9 cut(s)	597, 960, 1390, 1464, 1722, 1824, 2375, 2454, 2522
BtgI	CCRYGG	1 cut(s)	858
BtgZI	GCGATG	2 cut(s)	1077, 1594
BtsI	GCAGTG	1 cut(s)	1239
BtsIMutI	CAGTG	8 cut(s)	27, 95, 1057, 1230, 1239, 1462, 2178, 2658
BtuMI	TCGCGA	1 cut(s)	2110
BveI	ACCTGC	2 cut(s)	836, 1813
Cac8I	GCNNGC	9 cut(s)	339, 376, 501, 979, 1554, 1591, 1844, 2687, 2691
Cfr10I	RCCGGY	2 cut(s)	747, 1212
Cfr13I	GGNCC	7 cut(s)	455, 595, 596, 890, 1316, 1823, 2453
Cfr42I	CCGCGG	1 cut(s)	861
Cfr9I	CCCGGG	1 cut(s)	1303
CsiI	ACCWGGT	1 cut(s)	892
Csp6I	GTAC	5 cut(s)	685, 883, 1704, 1884, 2170
CviQI	GTAC	5 cut(s)	685, 883, 1704, 1884, 2170
DdeI	CTNAG	8 cut(s)	321, 731, 743, 1890, 2071, 2145, 2508, 2635
EaeI	YGGCCR	4 cut(s)	958, 1388, 1462, 2520
EagI	CGGCCG	1 cut(s)	2520
Eam1104I	CTCTTC	4 cut(s)	418, 2270, 2481, 2661
EarI	CTCTTC	4 cut(s)	418, 2270, 2481, 2661
EciI	GGCGGA	1 cut(s)	1965
Ecl136II	GAGCTC	2 cut(s)	503, 1058
EclXI	CGGCCG	1 cut(s)	2520
Eco130I	CCWWGG	1 cut(s)	2680
Eco24I	GRGCYC	3 cut(s)	505, 599, 1060
Eco47I	GGWCC	3 cut(s)	455, 890, 1316
Eco52I	CGGCCG	1 cut(s)	2520
Eco53kI	GAGCTC	2 cut(s)	503, 1058
Eco57I	CTGAAG	2 cut(s)	734, 2266
Eco72I	CACGTG	1 cut(s)	2032
Eco88I	CYCGRG	1 cut(s)	1303
Eco91I	GGTNACC	1 cut(s)	2101
EcoICRI	GAGCTC	2 cut(s)	503, 1058
EcoNI	CCTNNNNNAGG	2 cut(s)	1072, 2651
EcoO109I	RGGNCCY	1 cut(s)	595
EcoO65I	GGTNACC	1 cut(s)	2101
EcoRI	GAATTC	2 cut(s)	1931, 2012
EcoRII	CCWGG	6 cut(s)	892, 1073, 1294, 2401, 2455, 2646
EcoT14I	CCWWGG	1 cut(s)	2680
EcoT22I	ATGCAT	2 cut(s)	999, 1165
EcoT38I	GRGCYC	3 cut(s)	505, 599, 1060
ErhI	CCWWGG	1 cut(s)	2680
FalI	AAGNNNNNCTT	6 cut(s)	207, 239, 1536, 1568, 2264, 2296
FaqI	GGGAC	3 cut(s)	20, 441, 644
FauI	CCCGC	2 cut(s)	1209, 1577
FauNDI	CATATG	3 cut(s)	841, 1021, 1321
FbaI	TGATCA	2 cut(s)	283, 1324
Fnu4HI	GCNGC	9 cut(s)	15, 861, 909, 1532, 1535, 1952, 2520, 2745, 2748
FriOI	GRGCYC	3 cut(s)	505, 599, 1060
Fsp4HI	GCNGC	9 cut(s)	15, 861, 909, 1532, 1535, 1952, 2520, 2745, 2748
FspBI	CTAG	6 cut(s)	978, 1275, 1631, 1997, 2138, 2690
GluI	GCNGC	9 cut(s)	15, 861, 909, 1532, 1535, 1952, 2520, 2745, 2748
GsuI	CTGGAG	1 cut(s)	795
HaeIII	GGCC	9 cut(s)	597, 960, 1390, 1464, 1722, 1824, 2375, 2454, 2522
HapII	CCGG	6 cut(s)	62, 748, 1213, 1304, 1732, 2523
HindIII	AAGCTT	3 cut(s)	1417, 1861, 2732
HinfI	GANTC	7 cut(s)	8, 52, 203, 2195, 2249, 2433, 2643
HpaII	CCGG	6 cut(s)	62, 748, 1213, 1304, 1732, 2523
HphI	GGTGA	8 cut(s)	527, 1163, 1202, 1720, 1983, 2095, 2552, 2675
Hpy166II	GTNNAC	4 cut(s)	85, 899, 1171, 1991
Hpy8I	GTNNAC	4 cut(s)	85, 899, 1171, 1991
Hpy99I	CGWCG	2 cut(s)	1470, 1669
HpyAV	CCTTC	5 cut(s)	295, 2044, 2365, 2544, 2701
HpyCH4IV	ACGT	2 cut(s)	2031, 2716
HpyF3I	CTNAG	8 cut(s)	321, 731, 743, 1890, 2071, 2145, 2508, 2635
HpySE526I	ACGT	2 cut(s)	2031, 2716
Kpn2I	TCCGGA	1 cut(s)	61
Ksp22I	TGATCA	2 cut(s)	283, 1324
KspI	CCGCGG	1 cut(s)	861
LguI	GCTCTTC	2 cut(s)	2270, 2661
LmnI	GCTCC	8 cut(s)	64, 790, 1055, 1063, 1498, 1927, 2218, 2554
Lsp1109I	GCAGC	5 cut(s)	920, 1521, 1543, 1963, 2734
MabI	ACCWGGT	1 cut(s)	892
MaeI	CTAG	6 cut(s)	978, 1275, 1631, 1997, 2138, 2690
MaeII	ACGT	2 cut(s)	2031, 2716
MaeIII	GTNAC	5 cut(s)	1394, 1501, 1726, 1809, 2101
MflI	RGATCY	5 cut(s)	814, 1037, 1734, 2615, 2631
MhlI	GDGCHC	6 cut(s)	41, 505, 599, 1060, 1495, 2367
MlsI	TGGCCA	2 cut(s)	960, 1390
MluNI	TGGCCA	2 cut(s)	960, 1390
MlyI	GAGTC	2 cut(s)	2, 2652
MmeI	TCCRAC	4 cut(s)	132, 157, 1647, 2199
Mox20I	TGGCCA	2 cut(s)	960, 1390
Mph1103I	ATGCAT	2 cut(s)	999, 1165
MroI	TCCGGA	1 cut(s)	61
MroXI	GAANNNNTTC	1 cut(s)	2279
MscI	TGGCCA	2 cut(s)	960, 1390
MseI	TTAA	8 cut(s)	255, 1362, 1401, 1674, 2163, 2271, 2337, 2348
Msp20I	TGGCCA	2 cut(s)	960, 1390
MspA1I	CMGCKG	3 cut(s)	860, 1534, 2747
MspI	CCGG	6 cut(s)	62, 748, 1213, 1304, 1732, 2523
MspR9I	CCNGG	9 cut(s)	894, 1075, 1296, 1304, 1305, 1732, 2403, 2457, 2648
Mva1269I	GAATGC	2 cut(s)	418, 448
MvaI	CCWGG	6 cut(s)	894, 1075, 1296, 2403, 2457, 2648
MvnI	CGCG	2 cut(s)	860, 2110
NciI	CCSGG	3 cut(s)	1304, 1305, 1732
NdeI	CATATG	3 cut(s)	841, 1021, 1321
NheI	GCTAGC	2 cut(s)	977, 2689
NlaIV	GGNNCC	9 cut(s)	38, 457, 597, 639, 807, 1039, 1262, 1350, 1449
NmeAIII	GCCGAG	1 cut(s)	730
NmuCI	GTSAC	3 cut(s)	1394, 1726, 2101
NruI	TCGCGA	1 cut(s)	2110
NsiI	ATGCAT	2 cut(s)	999, 1165
NspI	RCATGY	4 cut(s)	917, 1048, 1509, 1593
NspV	TTCGAA	1 cut(s)	532
PaeI	GCATGC	1 cut(s)	1593
PaqCI	CACCTGC	1 cut(s)	836
PciI	ACATGT	1 cut(s)	1505
PciSI	GCTCTTC	2 cut(s)	2270, 2661
PcsI	WCGNNNNNNNCGW	1 cut(s)	375
PctI	GAATGC	2 cut(s)	418, 448
PdmI	GAANNNNTTC	1 cut(s)	2279
PfeI	GAWTC	5 cut(s)	52, 203, 2195, 2249, 2433
PflFI	GACNNNGTC	1 cut(s)	1470
PflMI	CCANNNNNTGG	2 cut(s)	1457, 1484
PfoI	TCCNGGA	3 cut(s)	1294, 2401, 2646
PkrI	GCNGC	9 cut(s)	16, 862, 910, 1533, 1536, 1953, 2521, 2746, 2749
PleI	GAGTC	2 cut(s)	2, 2651
PmaCI	CACGTG	1 cut(s)	2032
PmlI	CACGTG	1 cut(s)	2032
PpsI	GAGTC	2 cut(s)	2, 2651
Ppu21I	YACGTR	1 cut(s)	2032
PscI	ACATGT	1 cut(s)	1505
Psp124BI	GAGCTC	2 cut(s)	505, 1060
Psp6I	CCWGG	6 cut(s)	892, 1073, 1294, 2401, 2455, 2646
PspCI	CACGTG	1 cut(s)	2032
PspEI	GGTNACC	1 cut(s)	2101
PspGI	CCWGG	6 cut(s)	892, 1073, 1294, 2401, 2455, 2646
PspN4I	GGNNCC	9 cut(s)	38, 457, 597, 639, 807, 1039, 1262, 1350, 1449
PspOMI	GGGCCC	1 cut(s)	595
PspPI	GGNCC	7 cut(s)	455, 595, 596, 890, 1316, 1823, 2453
PsrI	GAACNNNNNNTAC	2 cut(s)	1794, 1826
PstI	CTGCAG	1 cut(s)	1533
PsuI	RGATCY	5 cut(s)	814, 1037, 1734, 2615, 2631
PsyI	GACNNNGTC	1 cut(s)	1470
PvuII	CAGCTG	2 cut(s)	1534, 2747
RruI	TCGCGA	1 cut(s)	2110
RsaI	GTAC	5 cut(s)	686, 884, 1705, 1885, 2171
RsaNI	GTAC	5 cut(s)	685, 883, 1704, 1884, 2170
SacI	GAGCTC	2 cut(s)	505, 1060
SacII	CCGCGG	1 cut(s)	861
SapI	GCTCTTC	2 cut(s)	2270, 2661
SaqAI	TTAA	8 cut(s)	255, 1362, 1401, 1674, 2163, 2271, 2337, 2348
SatI	GCNGC	9 cut(s)	15, 861, 909, 1532, 1535, 1952, 2520, 2745, 2748
Sau96I	GGNCC	7 cut(s)	455, 595, 596, 890, 1316, 1823, 2453
ScaI	AGTACT	3 cut(s)	884, 1705, 2171
SchI	GAGTC	2 cut(s)	2, 2652
ScrFI	CCNGG	9 cut(s)	894, 1075, 1296, 1304, 1305, 1732, 2403, 2457, 2648
SduI	GDGCHC	6 cut(s)	41, 505, 599, 1060, 1495, 2367
SexAI	ACCWGGT	1 cut(s)	892
SfcI	CTRYAG	3 cut(s)	18, 1529, 1914
Sfr303I	CCGCGG	1 cut(s)	861
SfuI	TTCGAA	1 cut(s)	532
SgrAI	CRCCGGYG	1 cut(s)	1212
SgrBI	CCGCGG	1 cut(s)	861
SinI	GGWCC	3 cut(s)	455, 890, 1316
SmaI	CCCGGG	1 cut(s)	1305
SmlI	CTYRAG	2 cut(s)	504, 2366
SmoI	CTYRAG	2 cut(s)	504, 2366
SpeI	ACTAGT	1 cut(s)	1630
SphI	GCATGC	1 cut(s)	1593
SsiI	CCGC	7 cut(s)	858, 860, 1216, 1584, 1976, 2059, 2519
SspI	AATATT	1 cut(s)	2411
SspMI	CTAG	6 cut(s)	978, 1275, 1631, 1997, 2138, 2690
SstI	GAGCTC	2 cut(s)	505, 1060
StyD4I	CCNGG	9 cut(s)	892, 1073, 1294, 1302, 1303, 1730, 2401, 2455, 2646
StyI	CCWWGG	1 cut(s)	2680
TaiI	ACGT	2 cut(s)	2034, 2719
TaqI	TCGA	7 cut(s)	115, 369, 472, 532, 875, 2021, 2628
TaqII	GACCGA	2 cut(s)	714, 1304
TatI	WGTACW	3 cut(s)	882, 1703, 2169
TauI	GCSGC	2 cut(s)	863, 2522
TfiI	GAWTC	5 cut(s)	52, 203, 2195, 2249, 2433
Tru1I	TTAA	8 cut(s)	255, 1362, 1401, 1674, 2163, 2271, 2337, 2348
Tru9I	TTAA	8 cut(s)	255, 1362, 1401, 1674, 2163, 2271, 2337, 2348
TscAI	CASTG	8 cut(s)	27, 95, 1057, 1230, 1246, 1462, 2185, 2665
TseFI	GTSAC	3 cut(s)	1394, 1726, 2101
TseI	GCWGC	7 cut(s)	14, 908, 1531, 1534, 1951, 2744, 2747
Tsp45I	GTSAC	3 cut(s)	1394, 1726, 2101
TspDTI	ATGAA	9 cut(s)	407, 417, 828, 978, 1415, 1856, 1863, 2241, 2744
TspGWI	ACGGA	1 cut(s)	1653
TspMI	CCCGGG	1 cut(s)	1303
TspRI	CASTG	8 cut(s)	27, 95, 1057, 1230, 1246, 1462, 2185, 2665
Tth111I	GACNNNGTC	1 cut(s)	1470
Van91I	CCANNNNNTGG	2 cut(s)	1457, 1484
VpaK11BI	GGWCC	3 cut(s)	455, 890, 1316
XagI	CCTNNNNNAGG	2 cut(s)	1072, 2651
XapI	RAATTY	4 cut(s)	1524, 1931, 1939, 2012
XbaI	TCTAGA	1 cut(s)	1996
XceI	RCATGY	4 cut(s)	917, 1048, 1509, 1593
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1049
XmaI	CCCGGG	1 cut(s)	1303
XmnI	GAANNNNTTC	1 cut(s)	2279
XspI	CTAG	6 cut(s)	978, 1275, 1631, 1997, 2138, 2690
ZrmI	AGTACT	3 cut(s)	884, 1705, 2171
Zsp2I	ATGCAT	2 cut(s)	999, 1165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.