MD01G1085300.v1.1

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012267
Physical Location & Seq
Reverse (-)
19184951 .. 19187695
2745 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1085300.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGAGTTCTGCCCAGGTTGTGGTAACATGCTGCAGTATGAACTGCCGAATATGCACGATGCGAGATTCTTCTGTCCAACTTGTCCCTATGTAGCATACGTTGATAGGAAGGTGAAGATTAAAAGAAGGCAGCATCTAGTTAAAAAAGAGATACAGCCCATCTTTACCCTGGACGACTACAAGAATGCGCCCAAAACTGAAGAACCGTGCCCAAGATGCGGGTTTCCTGAGGCGGCTTACAGGACACAGCAAACACGATCTGCCGATGAACCAGAAACAAGATTCTACAGGTGTATGAACAACAATTGCGGACACACATGGGTTGATTACACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00230 ko00240 ko01100 ko03020 ko04623 ko05169 map00230 map00240 map01100 map03020 map04623 map05169
Pfam Domains
Protein Families
Nudix_N_2 RNA_POL_M_15KD TFIIS_C

Protein Analysis

112

Amino Acids

13.15

Weight (kDa)

8.11

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_TFIIS PF01096 69 - 107 1.5e-16 Transcription factor S-II (TFIIS)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 20
AciI CCGC 3 cut(s) 219, 233, 309
AcuI CTGAAG 1 cut(s) 219
AfiI CCNNNNNNNGG 2 cut(s) 20, 218
AjnI CCWGG 2 cut(s) 13, 168
ApeKI GCWGC 2 cut(s) 31, 130
AspLEI GCGC 1 cut(s) 190
AsuHPI GGTGA 1 cut(s) 124
AxyI CCTNAGG 1 cut(s) 228
BaeGI GKGCMC 1 cut(s) 212
BbvI GCAGC 2 cut(s) 18, 142
BccI CCATC 1 cut(s) 167
BciT130I CCWGG 2 cut(s) 15, 170
BfaI CTAG 1 cut(s) 137
BfmI CTRYAG 2 cut(s) 32, 286
BisI GCNGC 3 cut(s) 32, 131, 234
BlsI GCNGC 3 cut(s) 33, 132, 235
Bme1390I CCNGG 2 cut(s) 15, 170
BmrFI CCNGG 2 cut(s) 15, 170
BmsI GCATC 3 cut(s) 49, 142, 206
BsaJI CCNNGG 2 cut(s) 13, 168
Bsc4I CCNNNNNNNGG 2 cut(s) 20, 218
Bse21I CCTNAGG 1 cut(s) 228
BseBI CCWGG 2 cut(s) 15, 170
BseDI CCNNGG 2 cut(s) 13, 168
BseLI CCNNNNNNNGG 2 cut(s) 20, 218
BseMII CTCAG 1 cut(s) 219
BseSI GKGCMC 1 cut(s) 212
BseXI GCAGC 2 cut(s) 18, 142
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 2 cut(s) 20, 218
BsmFI GGGAC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 190
Bsp1286I GDGCHC 1 cut(s) 212
Bsp143I GATC 1 cut(s) 257
BspACI CCGC 3 cut(s) 219, 233, 309
BspCNI CTCAG 1 cut(s) 220
BspMAI CTGCAG 1 cut(s) 36
BssECI CCNNGG 2 cut(s) 13, 168
BssMI GATC 1 cut(s) 257
Bst2UI CCWGG 2 cut(s) 15, 170
Bst4CI ACNGT 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 228, 333
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 1 cut(s) 260
BstMBI GATC 1 cut(s) 257
BstMWI GCNNNNNNNGC 2 cut(s) 52, 216
BstNI CCWGG 2 cut(s) 15, 170
BstNSI RCATGY 1 cut(s) 31
BstSCI CCNGG 2 cut(s) 13, 168
BstSFI CTRYAG 2 cut(s) 32, 286
BstSLI GKGCMC 1 cut(s) 212
BstV1I GCAGC 2 cut(s) 18, 142
Bsu36I CCTNAGG 1 cut(s) 228
CfoI GCGC 1 cut(s) 190
CviAII CATG 2 cut(s) 28, 318
CviJI RGCY 2 cut(s) 157, 236
CviKI_1 RGCY 2 cut(s) 157, 236
DdeI CTNAG 2 cut(s) 228, 333
DpnI GATC 1 cut(s) 259
DpnII GATC 1 cut(s) 257
Eco57I CTGAAG 1 cut(s) 219
Eco81I CCTNAGG 1 cut(s) 228
EcoRII CCWGG 2 cut(s) 13, 168
FaeI CATG 2 cut(s) 31, 321
FaiI YATR 7 cut(s) 29, 39, 53, 90, 97, 296, 319
FaqI GGGAC 1 cut(s) 69
FatI CATG 2 cut(s) 27, 317
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 32, 131, 234
Fsp4HI GCNGC 3 cut(s) 32, 131, 234
FspBI CTAG 1 cut(s) 137
GlaI GCGC 1 cut(s) 189
GluI GCNGC 3 cut(s) 32, 131, 234
HhaI GCGC 1 cut(s) 190
Hin1II CATG 2 cut(s) 31, 321
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HinfI GANTC 2 cut(s) 66, 282
HphI GGTGA 1 cut(s) 124
Hpy188III TCNNGA 1 cut(s) 227
HpyAV CCTTC 2 cut(s) 103, 120
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 1 cut(s) 99
HpyCH4V TGCA 2 cut(s) 34, 55
HpyF10VI GCNNNNNNNGC 2 cut(s) 52, 216
HpyF3I CTNAG 2 cut(s) 228, 333
HpySE526I ACGT 1 cut(s) 99
Hsp92II CATG 2 cut(s) 31, 321
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 1 cut(s) 257
LpnPI CCDG 7 cut(s) 27, 155, 182, 226, 240, 274, 285
Lsp1109I GCAGC 2 cut(s) 18, 142
LweI GCATC 3 cut(s) 49, 142, 206
MaeI CTAG 1 cut(s) 137
MaeII ACGT 1 cut(s) 99
MaeIII GTNAC 1 cut(s) 23
MalI GATC 1 cut(s) 259
MboI GATC 1 cut(s) 257
MboII GAAGA 3 cut(s) 61, 127, 212
MfeI CAATTG 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 212
MluCI AATT 1 cut(s) 304
MmeI TCCRAC 1 cut(s) 101
MnlI CCTC 1 cut(s) 223
MseI TTAA 2 cut(s) 120, 141
MspR9I CCNGG 2 cut(s) 15, 170
MunI CAATTG 1 cut(s) 304
Mva1269I GAATGC 1 cut(s) 190
MvaI CCWGG 2 cut(s) 15, 170
MwoI GCNNNNNNNGC 2 cut(s) 52, 216
NdeII GATC 1 cut(s) 257
NlaIII CATG 2 cut(s) 31, 321
NspI RCATGY 1 cut(s) 31
PctI GAATGC 1 cut(s) 190
PfeI GAWTC 2 cut(s) 66, 282
PflMI CCANNNNNTGG 1 cut(s) 20
PkrI GCNGC 3 cut(s) 33, 132, 235
Psp6I CCWGG 2 cut(s) 13, 168
PspGI CCWGG 2 cut(s) 13, 168
PstI CTGCAG 1 cut(s) 36
SaqAI TTAA 2 cut(s) 120, 141
SatI GCNGC 3 cut(s) 32, 131, 234
Sau3AI GATC 1 cut(s) 257
ScrFI CCNGG 2 cut(s) 15, 170
SduI GDGCHC 1 cut(s) 212
SetI ASST 4 cut(s) 19, 102, 114, 293
SfaNI GCATC 3 cut(s) 49, 142, 206
SfcI CTRYAG 2 cut(s) 32, 286
Sse9I AATT 1 cut(s) 304
SsiI CCGC 3 cut(s) 219, 233, 309
SspMI CTAG 1 cut(s) 137
StyD4I CCNGG 2 cut(s) 13, 168
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 1 cut(s) 102
TasI AATT 1 cut(s) 304
TauI GCSGC 1 cut(s) 236
TfiI GAWTC 2 cut(s) 66, 282
Tru1I TTAA 2 cut(s) 120, 141
Tru9I TTAA 2 cut(s) 120, 141
TseI GCWGC 2 cut(s) 31, 130
TspDTI ATGAA 3 cut(s) 54, 282, 311
Van91I CCANNNNNTGG 1 cut(s) 20
XceI RCATGY 1 cut(s) 31
XcmI CCANNNNNNNNNTGG 1 cut(s) 166
XspI CTAG 1 cut(s) 137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.