MD01G1102400.v1.1

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0012853
Physical Location & Seq
Forward (+)
21476750 .. 21480318
3569 bp
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UTR
Exon/CDS
Intron
MD01G1102400.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGAGACAATGTCAGTGAATACTGGAAATTCCCATTCCAATAAAAGGCAAGCGTCAGAGCATTCGGAAACTACTAATGAAGAAAAGAGGCCTGCAGTAAAAGAAGTGAGCACTCCTGTATTTGTTAATCATGCTGCAATTGCTTGGCATGAGAGGAGAAAAAAGTGGGTTGGGAATCAACGGGTGCAAAGAGCGGAAAAGGATCCAATCATAAGCTGGTCAACATCATATGAAGATCTGCTGTCGACGAATGAATCTTTTCCTGAGCCAATCCCCTTAACTGAGATGGTAGACTTTTTAGTTGATATTTGGCACGACGAAGGTCTCTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

112

Amino Acids

12.82

Weight (kDa)

5.17

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 11 - 67 5.5e-06 Gag1-like, clamp domain
clamp_Gag1-like PF13259 72 - 111 3.1e-10 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54880
fragaria_vesca FvH4_7g18580 FvH4_7g18670
malus_domestica MD01G1102400.v1.1
prunus_persica Prupe.2G208000_v2.0.a1 Prupe.2G208000_v2.0.a1 Prupe.2G208000_v2.0.a1
pyrus_communis pycom01g12880
rosa_chinensis RchiOBHm_Chr1g0362401
rosa_laevigata RLG00000027649
rosa_multiflora Rmu_sc0013947.1_g000008
rosa_roxburghii Rroxscaffold_4G00294210
rosa_rugosa Rorug01G0297800
rosa_samantha Rh1AG307000 Rh1BG270400 Rh1CG287900 Rh1DG300600
rosa_wichuraiana Rw1G027260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 194
AccI GTMKAC 2 cut(s) 245, 291
AciI CCGC 1 cut(s) 194
AclWI GGATC 2 cut(s) 197, 210
AcsI RAATTY 1 cut(s) 28
AfiI CCNNNNNNNGG 1 cut(s) 45
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
Alw21I GWGCWC 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 2 cut(s) 197, 210
AoxI GGCC 1 cut(s) 89
ApeKI GCWGC 1 cut(s) 134
ApoI RAATTY 1 cut(s) 28
Asp700I GAANNNNTTC 1 cut(s) 258
BamHI GGATCC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 113
BbvI GCAGC 1 cut(s) 121
BccI CCATC 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 329
BfmI CTRYAG 1 cut(s) 93
BglII AGATCT 1 cut(s) 235
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 204
BoxI GACNNNNGTC 1 cut(s) 321
Bpu10I CCTNAGC 1 cut(s) 264
BsaI GGTCTC 1 cut(s) 329
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 1 cut(s) 45
Bse1I ACTGG 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 45
BseMII CTCAG 2 cut(s) 255, 273
BseNI ACTGG 1 cut(s) 28
BseRI GAGGAG 1 cut(s) 169
BseXI GCAGC 1 cut(s) 121
BshFI GGCC 1 cut(s) 91
BsiHKAI GWGCWC 1 cut(s) 113
BslI CCNNNNNNNGG 1 cut(s) 45
BsmAI GTCTC 1 cut(s) 329
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 1 cut(s) 91
Bso31I GGTCTC 1 cut(s) 329
Bsp1286I GDGCHC 1 cut(s) 113
Bsp143I GATC 2 cut(s) 202, 235
BspACI CCGC 1 cut(s) 194
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 2 cut(s) 256, 274
BspLI GGNNCC 1 cut(s) 204
BspMAI CTGCAG 1 cut(s) 97
BspPI GGATC 2 cut(s) 197, 210
BspTNI GGTCTC 1 cut(s) 329
BsrBI CCGCTC 1 cut(s) 194
BsrI ACTGG 1 cut(s) 28
BssMI GATC 2 cut(s) 202, 235
Bst6I CTCTTC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 51, 93
BstDEI CTNAG 2 cut(s) 264, 282
BstKTI GATC 2 cut(s) 205, 238
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 2 cut(s) 202, 235
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstPAI GACNNNNGTC 1 cut(s) 321
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 1 cut(s) 121
BstX2I RGATCY 2 cut(s) 202, 235
BstYI RGATCY 2 cut(s) 202, 235
BsuRI GGCC 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 21
Cac8I GCNNGC 2 cut(s) 51, 93
CseI GACGC 1 cut(s) 42
CviAII CATG 2 cut(s) 131, 149
CviJI RGCY 3 cut(s) 91, 216, 268
CviKI_1 RGCY 3 cut(s) 91, 216, 268
DdeI CTNAG 2 cut(s) 264, 282
DpnI GATC 2 cut(s) 204, 237
DpnII GATC 2 cut(s) 202, 235
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Eco147I AGGCCT 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 329
FaeI CATG 2 cut(s) 134, 152
FaiI YATR 5 cut(s) 132, 150, 212, 229, 231
FatI CATG 2 cut(s) 130, 148
FauNDI CATATG 1 cut(s) 229
FblI GTMKAC 2 cut(s) 245, 291
Fnu4HI GCNGC 1 cut(s) 135
Fsp4HI GCNGC 1 cut(s) 135
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 1 cut(s) 91
HgaI GACGC 1 cut(s) 42
Hin1II CATG 2 cut(s) 134, 152
HincII GTYRAC 2 cut(s) 222, 246
HindII GTYRAC 2 cut(s) 222, 246
HinfI GANTC 2 cut(s) 175, 254
Hpy166II GTNNAC 3 cut(s) 222, 246, 292
Hpy188I TCNGA 2 cut(s) 58, 67
Hpy188III TCNNGA 1 cut(s) 263
Hpy8I GTNNAC 3 cut(s) 222, 246, 292
Hpy99I CGWCG 2 cut(s) 250, 320
HpyAV CCTTC 1 cut(s) 314
HpyCH4V TGCA 3 cut(s) 95, 137, 187
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 2 cut(s) 264, 282
Hsp92II CATG 2 cut(s) 134, 152
Kzo9I GATC 2 cut(s) 202, 235
LpnPI CCDG 5 cut(s) 9, 105, 129, 202, 276
Lsp1109I GCAGC 1 cut(s) 121
MalI GATC 2 cut(s) 204, 237
MbiI CCGCTC 1 cut(s) 194
MboI GATC 2 cut(s) 202, 235
MboII GAAGA 3 cut(s) 92, 245, 319
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 2 cut(s) 202, 235
MhlI GDGCHC 1 cut(s) 113
MluCI AATT 2 cut(s) 28, 138
MnlI CCTC 2 cut(s) 81, 147
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 2 cut(s) 126, 278
MunI CAATTG 1 cut(s) 138
Mva1269I GAATGC 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeI CATATG 1 cut(s) 229
NdeII GATC 2 cut(s) 202, 235
NlaIII CATG 2 cut(s) 134, 152
NlaIV GGNNCC 1 cut(s) 204
PceI AGGCCT 1 cut(s) 91
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 2 cut(s) 175, 254
PflFI GACNNNGTC 1 cut(s) 10
PkrI GCNGC 1 cut(s) 136
PshAI GACNNNNGTC 1 cut(s) 321
PspN4I GGNNCC 1 cut(s) 204
PstI CTGCAG 1 cut(s) 97
PsuI RGATCY 2 cut(s) 202, 235
PsyI GACNNNGTC 1 cut(s) 10
SalI GTCGAC 1 cut(s) 244
SaqAI TTAA 2 cut(s) 126, 278
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 2 cut(s) 202, 235
SduI GDGCHC 1 cut(s) 113
SetI ASST 2 cut(s) 218, 325
SfcI CTRYAG 1 cut(s) 93
Sse9I AATT 2 cut(s) 28, 138
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 1 cut(s) 194
StuI AGGCCT 1 cut(s) 91
TaqI TCGA 2 cut(s) 245, 330
TasI AATT 2 cut(s) 28, 138
TfiI GAWTC 2 cut(s) 175, 254
Tru1I TTAA 2 cut(s) 126, 278
Tru9I TTAA 2 cut(s) 126, 278
TscAI CASTG 1 cut(s) 21
TseI GCWGC 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 93, 246, 267
TspRI CASTG 1 cut(s) 21
Tth111I GACNNNGTC 1 cut(s) 10
XapI RAATTY 1 cut(s) 28
XcmI CCANNNNNNNNNTGG 1 cut(s) 213
XmiI GTMKAC 2 cut(s) 245, 291
XmnI GAANNNNTTC 1 cut(s) 258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.