RLG00000027649

Protein of unknown function (DUF4050)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0012853
Physical Location & Seq
Reverse (-)
13535023 .. 13536560
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027649

Sequence Viewer

Length: 321 bp
ATGCGTACTGGAAATTCCCGTTCCAATAAAAAGCAATCTTTAGAGAATAACAAATCTGCCACTGAAGAGAAAAGTCCTGCAGAAAAAGAAGCAAACACTTCTGTATTTGTCAACCATGCTGCAATTGCTTGGCATGACAATAGAAGAAAGTGGGTCGGGGATCAGCGGGTGCAAAGAATGGCCAAGGATCCAATTATAAGCTGGTCAACAGCGTATGAGGATCTACTTTCAACCAATGAGTGTTTCCCTGAGCCAATCCCTTTAACTGAGATGGTAGACTTTTTAGTTGATATTTGGCATGATGAAGGCCTGTTCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4050

Protein Analysis

107

Amino Acids

12.22

Weight (kDa)

5.16

Isoelectric Point (pI)

52.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
clamp_Gag1-like PF13259 67 - 106 5e-10 Gag1-like, clamp domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012853)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54880
fragaria_vesca FvH4_7g18580 FvH4_7g18670
malus_domestica MD01G1102400.v1.1
prunus_persica Prupe.2G208000_v2.0.a1 Prupe.2G208000_v2.0.a1 Prupe.2G208000_v2.0.a1
pyrus_communis pycom01g12880
rosa_chinensis RchiOBHm_Chr1g0362401
rosa_laevigata RLG00000027649
rosa_multiflora Rmu_sc0013947.1_g000008
rosa_roxburghii Rroxscaffold_4G00294210
rosa_rugosa Rorug01G0297800
rosa_samantha Rh1AG307000 Rh1BG270400 Rh1CG287900 Rh1DG300600
rosa_wichuraiana Rw1G027260

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 197
AccI GTMKAC 1 cut(s) 276
AciI CCGC 1 cut(s) 166
AclWI GGATC 4 cut(s) 168, 182, 195, 228
AcoI YGGCCR 1 cut(s) 180
AcsI RAATTY 1 cut(s) 13
AcuI CTGAAG 1 cut(s) 84
AfaI GTAC 1 cut(s) 7
AgsI TTSAA 1 cut(s) 231
AjuI GAANNNNNNNTTGG 2 cut(s) 227, 259
AluBI AGCT 1 cut(s) 201
AluI AGCT 1 cut(s) 201
AlwI GGATC 4 cut(s) 168, 182, 195, 228
AoxI GGCC 2 cut(s) 180, 307
ApeKI GCWGC 1 cut(s) 119
ApoI RAATTY 1 cut(s) 13
BalI TGGCCA 1 cut(s) 182
BamHI GGATCC 1 cut(s) 187
BbvI GCAGC 1 cut(s) 106
BccI CCATC 1 cut(s) 265
BfmI CTRYAG 1 cut(s) 78
BisI GCNGC 1 cut(s) 120
BlsI GCNGC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 249
BsaJI CCNNGG 1 cut(s) 183
Bse1I ACTGG 1 cut(s) 13
BseDI CCNNGG 1 cut(s) 183
BseMII CTCAG 2 cut(s) 240, 258
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 106
BshFI GGCC 2 cut(s) 182, 309
BsnI GGCC 2 cut(s) 182, 309
Bsp143I GATC 3 cut(s) 160, 187, 220
BspACI CCGC 1 cut(s) 166
BspANI GGCC 2 cut(s) 182, 309
BspCNI CTCAG 2 cut(s) 241, 259
BspLI GGNNCC 1 cut(s) 189
BspMAI CTGCAG 1 cut(s) 82
BspPI GGATC 4 cut(s) 168, 182, 195, 228
BsrI ACTGG 1 cut(s) 13
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 3 cut(s) 160, 187, 220
BssT1I CCWWGG 1 cut(s) 183
Bst6I CTCTTC 1 cut(s) 60
BstDEI CTNAG 2 cut(s) 249, 267
BstKTI GATC 3 cut(s) 163, 190, 223
BstMBI GATC 3 cut(s) 160, 187, 220
BstMWI GCNNNNNNNGC 1 cut(s) 125
BstSFI CTRYAG 1 cut(s) 78
BstV1I GCAGC 1 cut(s) 106
BstX2I RGATCY 2 cut(s) 187, 220
BstYI RGATCY 2 cut(s) 187, 220
BsuRI GGCC 2 cut(s) 182, 309
BtsIMutI CAGTG 1 cut(s) 60
Csp6I GTAC 1 cut(s) 6
CviAII CATG 3 cut(s) 116, 134, 299
CviJI RGCY 4 cut(s) 182, 201, 253, 309
CviKI_1 RGCY 4 cut(s) 182, 201, 253, 309
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 2 cut(s) 249, 267
DpnI GATC 3 cut(s) 162, 189, 222
DpnII GATC 3 cut(s) 160, 187, 220
EaeI YGGCCR 1 cut(s) 180
Eam1104I CTCTTC 1 cut(s) 60
EarI CTCTTC 1 cut(s) 60
Eco130I CCWWGG 1 cut(s) 183
Eco147I AGGCCT 1 cut(s) 309
Eco57I CTGAAG 1 cut(s) 84
EcoT14I CCWWGG 1 cut(s) 183
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 3 cut(s) 119, 137, 302
FaiI YATR 5 cut(s) 117, 135, 197, 216, 300
FatI CATG 3 cut(s) 115, 133, 298
FauI CCCGC 1 cut(s) 159
FblI GTMKAC 1 cut(s) 276
Fnu4HI GCNGC 1 cut(s) 120
Fsp4HI GCNGC 1 cut(s) 120
GluI GCNGC 1 cut(s) 120
HaeIII GGCC 2 cut(s) 182, 309
Hin1II CATG 3 cut(s) 119, 137, 302
HincII GTYRAC 2 cut(s) 112, 207
HindII GTYRAC 2 cut(s) 112, 207
Hpy166II GTNNAC 3 cut(s) 112, 207, 277
Hpy8I GTNNAC 3 cut(s) 112, 207, 277
HpyAV CCTTC 1 cut(s) 299
HpyCH4V TGCA 3 cut(s) 80, 122, 172
HpyF10VI GCNNNNNNNGC 1 cut(s) 125
HpyF3I CTNAG 2 cut(s) 249, 267
Hsp92II CATG 3 cut(s) 119, 137, 302
Kzo9I GATC 3 cut(s) 160, 187, 220
LpnPI CCDG 3 cut(s) 90, 187, 261
Lsp1109I GCAGC 1 cut(s) 106
MalI GATC 3 cut(s) 162, 189, 222
MboI GATC 3 cut(s) 160, 187, 220
MboII GAAGA 2 cut(s) 77, 156
MfeI CAATTG 1 cut(s) 123
MflI RGATCY 2 cut(s) 187, 220
MlsI TGGCCA 1 cut(s) 182
MluCI AATT 3 cut(s) 13, 123, 192
MluNI TGGCCA 1 cut(s) 182
MnlI CCTC 1 cut(s) 211
Mox20I TGGCCA 1 cut(s) 182
MscI TGGCCA 1 cut(s) 182
MseI TTAA 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 182
MspA1I CMGCKG 1 cut(s) 166
MunI CAATTG 1 cut(s) 123
MwoI GCNNNNNNNGC 1 cut(s) 125
NdeII GATC 3 cut(s) 160, 187, 220
NlaIII CATG 3 cut(s) 119, 137, 302
NlaIV GGNNCC 1 cut(s) 189
PceI AGGCCT 1 cut(s) 309
PkrI GCNGC 1 cut(s) 121
PsiI TTATAA 1 cut(s) 197
PspN4I GGNNCC 1 cut(s) 189
PstI CTGCAG 1 cut(s) 82
PsuI RGATCY 2 cut(s) 187, 220
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SaqAI TTAA 1 cut(s) 263
SatI GCNGC 1 cut(s) 120
Sau3AI GATC 3 cut(s) 160, 187, 220
SetI ASST 1 cut(s) 203
SfcI CTRYAG 1 cut(s) 78
Sse9I AATT 3 cut(s) 13, 123, 192
SseBI AGGCCT 1 cut(s) 309
SsiI CCGC 1 cut(s) 166
StuI AGGCCT 1 cut(s) 309
StyI CCWWGG 1 cut(s) 183
TaqI TCGA 1 cut(s) 315
TasI AATT 3 cut(s) 13, 123, 192
Tru1I TTAA 1 cut(s) 263
Tru9I TTAA 1 cut(s) 263
TscAI CASTG 1 cut(s) 67
TseI GCWGC 1 cut(s) 119
TspDTI ATGAA 1 cut(s) 318
TspRI CASTG 1 cut(s) 67
XapI RAATTY 1 cut(s) 13
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.