Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0016923

Physical Location & Seq

Genomic Coordinates Reverse (-)

22310794 .. 22313309

Gene Structure 2516 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1110400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 576 bp

ATGGAGGTCCGACCCAACCCGACGGCCGACAACTCGTCGGCCATGCAGCAGCAGCGTCAGCGCCATACACCCGCTTCTAGTCAGAAAAAGCCGCCTGCTTCTTCCATCCCCGTCGACACCACCTCCGTTTCTCAAAGGCTTCAGAAGGAGTTGATGTCTCTCATGATGAGTGGAGGAGATCTTGGAGTATCGGCTTTTCCTGAAGGTGAGAGCATTTTTACATGGGTTGGCACAATCGAAGGTGGAAAAGGAACTATGTATGAGGGTTTTTCCTACAAGCTGTCTTTGCGTTTTCCTCTGGACTATCCTTTCAAGCCACCGCAAGTCAAGTTTGAGACAATGTGCTTCCATCCAAATGTTGATCAGTTTGGCAACATATGTCTTGATATCTTGCAGGACAAGTGGTCTTCAGCTTACGATTGCAGGACTATTCTACTCTCCATTCAAAGTCTGTTGGGAGAACCAAACCCGGAGAGCCCCCTCAACAGCTATGCTGCTGCTCTGTGGAGTAACAAGGAAGATTACAAAAAAATGGTCCACCGACAGTACTTTGCCGGAGAATCAATCGAGAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0007088 GO:0007346 GO:0008150 GO:0008152 GO:0008283 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0010564 GO:0010965 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019899 GO:0019941 GO:0030071 GO:0030163 GO:0031145 GO:0031625 GO:0032446 GO:0033043 GO:0033044 GO:0033045 GO:0033047 GO:0036211 GO:0043161 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044389 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051128 GO:0051603 GO:0051726 GO:0051783 GO:0051983 GO:0061630 GO:0061659 GO:0065007 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575 GO:1901987 GO:1901990 GO:1902099 GO:1905818

KEGG Pathways

Metabolic & Signaling

ko04120 map04120

Pfam Domains

Protein Families

UQ_con

Protein Analysis

192

Amino Acids

21.4

Weight (kDa)

5.63

Isoelectric Point (pI)

59.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
UQ_con	PF00179	46 - 181	5.7e-45	Ubiquitin-conjugating enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0016923)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g19780
malus_domestica	MD01G1110400.v1.1 MD07G1176200.v1.1
prunus_persica	Prupe.2G215000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr1g0363711
rosa_laevigata	RLG00000027551
rosa_multiflora	Rmu_co8478827.1_g000001 Rmu_sc0000548.1_g000015
rosa_roxburghii	Rroxscaffold_4G00292980
rosa_rugosa	Rorug01G0308600 Rorug01G0308700 Rorug01G0308800
rosa_samantha	Rh1CG297300
rosa_wichuraiana	Rw1G028170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	114
AciI	CCGC	3 cut(s)	72, 92, 320
AcoI	YGGCCR	2 cut(s)	24, 39
AcuI	CTGAAG	3 cut(s)	125, 222, 393
AfaI	GTAC	1 cut(s)	548
AgsI	TTSAA	2 cut(s)	313, 446
AhdI	GACNNNNNGTC	2 cut(s)	34, 403
AluBI	AGCT	4 cut(s)	280, 413, 489, 573
AluI	AGCT	4 cut(s)	280, 413, 489, 573
Alw26I	GTCTC	2 cut(s)	162, 329
AoxI	GGCC	2 cut(s)	24, 39
ApeKI	GCWGC	5 cut(s)	46, 49, 52, 494, 497
ArsI	GACNNNNNNTTYG	2 cut(s)	519, 551
AspLEI	GCGC	1 cut(s)	63
AspS9I	GGNCC	2 cut(s)	7, 535
AsuC2I	CCSGG	1 cut(s)	470
AsuHPI	GGTGA	1 cut(s)	218
AvaII	GGWCC	2 cut(s)	7, 535
BanII	GRGCYC	1 cut(s)	479
BbsI	GAAGAC	1 cut(s)	399
BbvI	GCAGC	5 cut(s)	58, 61, 64, 481, 484
BccI	CCATC	2 cut(s)	113, 357
BceAI	ACGGC	1 cut(s)	39
BclI	TGATCA	1 cut(s)	361
BcnI	CCSGG	1 cut(s)	470
BcoDI	GTCTC	2 cut(s)	162, 329
BfaI	CTAG	1 cut(s)	78
BfoI	RGCGCY	1 cut(s)	64
BglII	AGATCT	1 cut(s)	178
BisI	GCNGC	6 cut(s)	47, 50, 53, 92, 495, 498
BlsI	GCNGC	6 cut(s)	48, 51, 54, 93, 496, 499
BmcAI	AGTACT	1 cut(s)	548
Bme1390I	CCNGG	1 cut(s)	470
Bme18I	GGWCC	2 cut(s)	7, 535
BmeRI	GACNNNNNGTC	2 cut(s)	34, 403
BmgT120I	GGNCC	2 cut(s)	7, 535
BmrFI	CCNGG	1 cut(s)	470
BpiI	GAAGAC	1 cut(s)	399
BpuMI	CCSGG	1 cut(s)	470
BsaXI	ACNNNNNCTCC	4 cut(s)	107, 137, 140, 170
BseGI	GGATG	2 cut(s)	105, 349
BseRI	GAGGAG	1 cut(s)	189
BseX3I	CGGCCG	1 cut(s)	24
BseXI	GCAGC	5 cut(s)	58, 61, 64, 481, 484
Bsh1285I	CGRYCG	1 cut(s)	27
BshFI	GGCC	2 cut(s)	26, 41
BsiEI	CGRYCG	1 cut(s)	27
BsiSI	CCGG	2 cut(s)	470, 555
BsmAI	GTCTC	2 cut(s)	162, 329
BsnI	GGCC	2 cut(s)	26, 41
Bsp1286I	GDGCHC	1 cut(s)	479
Bsp143I	GATC	2 cut(s)	178, 361
BspACI	CCGC	3 cut(s)	72, 92, 320
BspANI	GGCC	2 cut(s)	26, 41
BspHI	TCATGA	1 cut(s)	162
BssMI	GATC	2 cut(s)	178, 361
Bst4CI	ACNGT	1 cut(s)	546
BstC8I	GCNNGC	1 cut(s)	96
BstF5I	GGATG	2 cut(s)	105, 349
BstH2I	RGCGCY	1 cut(s)	64
BstHHI	GCGC	1 cut(s)	63
BstKTI	GATC	2 cut(s)	181, 364
BstMAI	GTCTC	2 cut(s)	162, 329
BstMBI	GATC	2 cut(s)	178, 361
BstMCI	CGRYCG	1 cut(s)	27
BstMWI	GCNNNNNNNGC	3 cut(s)	52, 58, 286
BstSCI	CCNGG	1 cut(s)	468
BstV1I	GCAGC	5 cut(s)	58, 61, 64, 481, 484
BstV2I	GAAGAC	1 cut(s)	399
BstX2I	RGATCY	1 cut(s)	178
BstYI	RGATCY	1 cut(s)	178
BstZI	CGGCCG	1 cut(s)	24
BsuRI	GGCC	2 cut(s)	26, 41
BtsCI	GGATG	2 cut(s)	105, 349
Cac8I	GCNNGC	1 cut(s)	96
CciI	TCATGA	1 cut(s)	162
CfoI	GCGC	1 cut(s)	63
Cfr13I	GGNCC	2 cut(s)	7, 535
CseI	GACGC	1 cut(s)	44
Csp6I	GTAC	1 cut(s)	547
CviAII	CATG	3 cut(s)	43, 163, 222
CviQI	GTAC	1 cut(s)	547
DpnI	GATC	2 cut(s)	180, 363
DpnII	GATC	2 cut(s)	178, 361
DriI	GACNNNNNGTC	2 cut(s)	34, 403
EaeI	YGGCCR	2 cut(s)	24, 39
EagI	CGGCCG	1 cut(s)	24
Eam1105I	GACNNNNNGTC	2 cut(s)	34, 403
EclXI	CGGCCG	1 cut(s)	24
Eco24I	GRGCYC	1 cut(s)	479
Eco32I	GATATC	1 cut(s)	388
Eco47I	GGWCC	2 cut(s)	7, 535
Eco52I	CGGCCG	1 cut(s)	24
Eco57I	CTGAAG	3 cut(s)	125, 222, 393
EcoRV	GATATC	1 cut(s)	388
EcoT38I	GRGCYC	1 cut(s)	479
FaeI	CATG	3 cut(s)	46, 166, 225
FaiI	YATR	9 cut(s)	44, 66, 164, 223, 257, 261, 377, 379, 492
FatI	CATG	3 cut(s)	42, 162, 221
FauI	CCCGC	1 cut(s)	79
FauNDI	CATATG	1 cut(s)	377
FbaI	TGATCA	1 cut(s)	361
FblI	GTMKAC	1 cut(s)	114
Fnu4HI	GCNGC	6 cut(s)	47, 50, 53, 92, 495, 498
FokI	GGATG	2 cut(s)	92, 336
FriOI	GRGCYC	1 cut(s)	479
Fsp4HI	GCNGC	6 cut(s)	47, 50, 53, 92, 495, 498
FspBI	CTAG	1 cut(s)	78
GlaI	GCGC	1 cut(s)	62
GluI	GCNGC	6 cut(s)	47, 50, 53, 92, 495, 498
HaeII	RGCGCY	1 cut(s)	64
HaeIII	GGCC	2 cut(s)	26, 41
HapII	CCGG	2 cut(s)	470, 555
HgaI	GACGC	1 cut(s)	44
HhaI	GCGC	1 cut(s)	63
Hin1II	CATG	3 cut(s)	46, 166, 225
Hin6I	GCGC	1 cut(s)	61
HinP1I	GCGC	1 cut(s)	61
HincII	GTYRAC	1 cut(s)	115
HindII	GTYRAC	1 cut(s)	115
HinfI	GANTC	1 cut(s)	560
HpaII	CCGG	2 cut(s)	470, 555
HphI	GGTGA	1 cut(s)	218
Hpy166II	GTNNAC	2 cut(s)	115, 538
Hpy188I	TCNGA	3 cut(s)	11, 84, 144
Hpy188III	TCNNGA	5 cut(s)	163, 200, 299, 383, 568
Hpy8I	GTNNAC	2 cut(s)	115, 538
Hpy99I	CGWCG	3 cut(s)	25, 40, 116
HpyAV	CCTTC	3 cut(s)	139, 197, 233
HpyCH4III	ACNGT	1 cut(s)	546
HpyCH4V	TGCA	3 cut(s)	46, 394, 423
HpyF10VI	GCNNNNNNNGC	3 cut(s)	52, 58, 286
Hsp92II	CATG	3 cut(s)	46, 166, 225
HspAI	GCGC	1 cut(s)	61
Ksp22I	TGATCA	1 cut(s)	361
Kzo9I	GATC	2 cut(s)	178, 361
LpnPI	CCDG	7 cut(s)	108, 213, 284, 380, 409, 483, 568
Lsp1109I	GCAGC	5 cut(s)	58, 61, 64, 481, 484
MaeI	CTAG	1 cut(s)	78
MaeIII	GTNAC	1 cut(s)	509
MalI	GATC	2 cut(s)	180, 363
MboI	GATC	2 cut(s)	178, 361
MboII	GAAGA	3 cut(s)	93, 399, 530
MflI	RGATCY	1 cut(s)	178
MhlI	GDGCHC	1 cut(s)	479
MmeI	TCCRAC	1 cut(s)	34
MnlI	CCTC	5 cut(s)	133, 167, 256, 306, 491
MslI	CAYNNNNRTG	1 cut(s)	354
MspI	CCGG	2 cut(s)	470, 555
MspR9I	CCNGG	1 cut(s)	470
MwoI	GCNNNNNNNGC	3 cut(s)	52, 58, 286
NciI	CCSGG	1 cut(s)	470
NdeI	CATATG	1 cut(s)	377
NdeII	GATC	2 cut(s)	178, 361
NlaIII	CATG	3 cut(s)	46, 166, 225
PagI	TCATGA	1 cut(s)	162
PfeI	GAWTC	1 cut(s)	560
PkrI	GCNGC	6 cut(s)	48, 51, 54, 93, 496, 499
PspPI	GGNCC	2 cut(s)	7, 535
PsuI	RGATCY	1 cut(s)	178
RsaI	GTAC	1 cut(s)	548
RsaNI	GTAC	1 cut(s)	547
RseI	CAYNNNNRTG	1 cut(s)	354
SalI	GTCGAC	1 cut(s)	113
SatI	GCNGC	6 cut(s)	47, 50, 53, 92, 495, 498
Sau3AI	GATC	2 cut(s)	178, 361
Sau96I	GGNCC	2 cut(s)	7, 535
ScaI	AGTACT	1 cut(s)	548
ScrFI	CCNGG	1 cut(s)	470
SduI	GDGCHC	1 cut(s)	479
SetI	ASST	8 cut(s)	9, 125, 208, 244, 282, 415, 491, 575
SinI	GGWCC	2 cut(s)	7, 535
SmiMI	CAYNNNNRTG	1 cut(s)	354
SsiI	CCGC	3 cut(s)	72, 92, 320
SspMI	CTAG	1 cut(s)	78
StyD4I	CCNGG	1 cut(s)	468
TaaI	ACNGT	1 cut(s)	546
TaqI	TCGA	3 cut(s)	114, 237, 567
TatI	WGTACW	1 cut(s)	546
TauI	GCSGC	1 cut(s)	94
TfiI	GAWTC	1 cut(s)	560
TseI	GCWGC	5 cut(s)	46, 49, 52, 494, 497
TspGWI	ACGGA	1 cut(s)	115
VpaK11BI	GGWCC	2 cut(s)	7, 535
XmiI	GTMKAC	1 cut(s)	114
XspI	CTAG	1 cut(s)	78
ZrmI	AGTACT	1 cut(s)	548

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.