Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010764

Physical Location & Seq

Genomic Coordinates Reverse (-)

23295311 .. 23299333

Gene Structure 4023 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1118500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGGCGTCCCCAATCGAACAAGGAGGTGATGGAGACTTAGAGAAGGGTCCGATGACTGCACAGCCTAACGCGGAACCATCGCCGTCGCCGTCTCCGTCTTCCACGGCGTCGGCGCCGGCTTTGGTGCTTTCCAACTCTGGAAAGCGGATGGACCAGGCGGGGAAGAAGAAGTACGTGAAGCAGGTCACTGGGCGGCACAACGACACGGAGCTCCACTTGGCGGCTCAGCGCGCGGATCTAGCCGCCGTGAAGAAGATTCTCGGCGATATCGATTCGCAGATTGTGGGGACAGTTAGCGGTGCGGAGTTTGATACGGAGGTGGCTGAGGTCCGGGCGGCGGTGGTGAATGAGGTCAATGAGCTCGGAGAGACGGCTTTGTTCACGGCGGCGGATAAAGGGCACCTTGATGTGGTTAAGGAGCTGTTGAAATATTCGAATCAGGAGACCGTTACGAAGAAGAACCGGTCCGGGTTTGATCCCTTGCATGCTGCTGCAAGTCAAGGACACCATGCCATTGTCCAGGTGCTCCTAGATCATGACCCTGGGCTAAGCAAAACAATTGGTCCGTCGAATTCAACCCCACTTATATCTGCAGCTCATAAAGGGCATATAGCAGTAGTTGATGAACTGCTCTCGAAAGATTCTACCTTGTTGGAGATTTCTAAATCAAATGGGAAGAATGCATTGCATCTGGCTGCAAGACAGGGGCATACAGAGATTGTAAGCGCATTGCTAACTAAGGATCCACAGTTGGCACGAAGAACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGACGAACTGTGAGGTGGTGAAATTGCTTCTTGAGGCAGACCCCGCAATTGTAATGCTGCCAGACAAATTTGGTAACACAGCATTACATATTGCCACCAGGAAGAGGCGAGCAGAGATAGTGAATGAGTTGTTACTCCTCCCAGACACCAATGTTAATGCACTGAACAGGGACACAAAAACGGCTCTTGACATTGCTGATGCACTTCCACTTTCCGAAGAATCCTCAGAAATAAGGGGTTGTCTTTATCGCTATGGTGCAGTCAAGGCAAATGAACTGAACCAACCAAGGGATGAATTGAGGAAAACGGTGACTCAGATCAAGAACGATGTTCATATCCAGCTTGAACAAACAAAGAAAACCAACAAAAATGTTCACAATATATCCAAAGAACTGAGAAAGCTCCACAGGGAAGGAATCAACAACGCCACCAACTCAGTCACTGTGGTGGCTGTTCTATTCGCAACAGTTGCGTTTGCGGCTATCTTTACTGTGCCCGGTGGTGATAATGATGACGGGACTGCCGTGGTAGTTAAGAGCACTCCTTTCAAAATCTTCTTCATTTTCAATGCCATTGCCCTTTTTACGTCGCTGGCTGTTGTGGTTGTCCAAATTACCTTGGTCAGAGGCGAGACAAAGGCAGAGAAACGGGTGGTGGAGATAATCAACAAGTTGATGTGGCTGGCTTCTGTCTGCACTTCAGTGGCATTCATGGCCTCCTCTTACATAGTGGTTGGCCGGCACCATAAGTGGGCCGCCATTCTGGTAACAGTCGTTGGGGGAATGATAATGGCTGCTGTTCTCGGCACCATGACATACTACGTAGTGAAGTCGAAGCGGATACGTTCCATGAGGAAGAAGCACCCGAAGAGGAGCGGTTCCAACTCGTGGATGCACTCTGACCATTCAAATTCGGAAATTGAACGGATTTATGCCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0009628 GO:0009651 GO:0009987 GO:0016020 GO:0023052 GO:0033036 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0065007 GO:0070727 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 PGG

Protein Analysis

582

Amino Acids

62.91

Weight (kDa)

9.19

Isoelectric Point (pI)

35.5

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	71 - 180	1.6e-14	Ankyrin repeats (3 copies)
Ank_KRIT1	PF24521	122 - 214	2.6e-07	KRIT1 ankyrin-repeats domain
Ank_4	PF13637	126 - 177	5.1e-08	Ankyrin repeats (many copies)
Ank_2	PF12796	190 - 248	9.6e-11	Ankyrin repeats (3 copies)
Ank_2	PF12796	195 - 282	4.6e-17	Ankyrin repeats (3 copies)
Ank_4	PF13637	196 - 245	7.6e-06	Ankyrin repeats (many copies)
Ank_KRIT1	PF24521	217 - 315	8.7e-06	KRIT1 ankyrin-repeats domain
Ank_2	PF12796	254 - 321	1.4e-11	Ankyrin repeats (3 copies)
Ank_5	PF13857	278 - 334	4.6e-08	Ankyrin repeats (many copies)
PGG	PF13962	406 - 512	2.9e-29	Domain of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0010764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12360 AT3G12360
fragaria_vesca	FvH4_7g20900
malus_domestica	MD01G1118500.v1.1 MD07G1186700.v1.1
prunus_persica	Prupe.2G225600_v2.0.a1
pyrus_communis	pycom01g14570
rosa_chinensis	RchiOBHm_Chr1g0365211
rosa_laevigata	RLG00000027438
rosa_multiflora	Rmu_co8385283.1_g000001 Rmu_sc0008035.1_g000005 Rmu_sc0024070.1_g000002
rosa_roxburghii	Rroxscaffold_4G00291370 Rroxscaffold_4G00291910
rosa_rugosa	Rorug01G0320300
rosa_samantha	Rh1AG329300 Rh1BG290700 Rh1CG307600 Rh1DG322400
rosa_wichuraiana	Rw1G029200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	172
AccB1I	GGYRCC	4 cut(s)	112, 399, 1545, 1610
AccB7I	CCANNNNNTGG	1 cut(s)	1692
AccBSI	CCGCTC	1 cut(s)	1680
AccII	CGCG	3 cut(s)	71, 231, 233
AclWI	GGATC	4 cut(s)	243, 470, 737, 750
AcoI	YGGCCR	1 cut(s)	1540
AcsI	RAATTY	3 cut(s)	571, 872, 1714
AcuI	CTGAAG	1 cut(s)	1488
AcyI	GRCGYC	3 cut(s)	5, 107, 113
AfaI	GTAC	1 cut(s)	173
AfiI	CCNNNNNNNGG	7 cut(s)	220, 337, 409, 909, 1093, 1555, 1692
AgeI	ACCGGT	1 cut(s)	462
AgsI	TTSAA	7 cut(s)	427, 576, 1151, 1354, 1372, 1713, 1727
AjnI	CCWGG	4 cut(s)	153, 519, 541, 902
AleI	CACNNNNGTG	2 cut(s)	1250, 1505
AluBI	AGCT	6 cut(s)	211, 361, 421, 596, 1147, 1207
AluI	AGCT	6 cut(s)	211, 361, 421, 596, 1147, 1207
Alw21I	GWGCWC	4 cut(s)	213, 363, 528, 1346
Alw26I	GTCTC	5 cut(s)	27, 96, 362, 437, 1430
AlwI	GGATC	4 cut(s)	243, 470, 737, 750
AlwNI	CAGNNNCTG	1 cut(s)	1247
AoxI	GGCC	3 cut(s)	1518, 1540, 1557
ApeKI	GCWGC	6 cut(s)	488, 491, 593, 695, 862, 1598
ApoI	RAATTY	3 cut(s)	571, 872, 1714
AsiGI	ACCGGT	1 cut(s)	462
AspLEI	GCGC	4 cut(s)	115, 231, 233, 728
AspS9I	GGNCC	6 cut(s)	47, 151, 328, 465, 563, 1557
AsuC2I	CCSGG	3 cut(s)	332, 469, 1302
AsuHPI	GGTGA	5 cut(s)	38, 355, 835, 1126, 1319
AsuII	TTCGAA	1 cut(s)	434
AvaII	GGWCC	5 cut(s)	47, 151, 328, 465, 563
BaeGI	GKGCMC	2 cut(s)	402, 1302
BaeI	ACNNNNGTAYC	2 cut(s)	303, 336
BamHI	GGATCC	1 cut(s)	742
BanI	GGYRCC	4 cut(s)	112, 399, 1545, 1610
BanII	GRGCYC	2 cut(s)	213, 363
BarI	GAAGNNNNNNTAC	2 cut(s)	155, 187
BauI	CACGAG	1 cut(s)	1690
BbsI	GAAGAC	1 cut(s)	90
Bbv12I	GWGCWC	4 cut(s)	213, 363, 528, 1346
BbvCI	CCTCAGC	1 cut(s)	324
BbvI	GCAGC	6 cut(s)	475, 478, 605, 682, 849, 1585
BccI	CCATC	3 cut(s)	23, 85, 142
BceAI	ACGGC	8 cut(s)	67, 73, 120, 230, 387, 399, 1002, 1313
BciT130I	CCWGG	4 cut(s)	155, 521, 543, 904
BciVI	GTATCC	1 cut(s)	1638
BcnI	CCSGG	3 cut(s)	332, 469, 1302
BcoDI	GTCTC	5 cut(s)	27, 96, 362, 437, 1430
BfaI	CTAG	2 cut(s)	239, 530
BfmI	CTRYAG	1 cut(s)	591
BfoI	RGCGCY	1 cut(s)	116
BfuAI	ACCTGC	1 cut(s)	172
BfuI	GTATCC	1 cut(s)	1638
BlpI	GCTNAGC	2 cut(s)	225, 548
Bme1390I	CCNGG	7 cut(s)	155, 332, 469, 521, 543, 904, 1302
Bme18I	GGWCC	5 cut(s)	47, 151, 328, 465, 563
BmgT120I	GGNCC	6 cut(s)	47, 151, 328, 465, 563, 1557
BmiI	GGNNCC	8 cut(s)	48, 75, 114, 401, 744, 1547, 1612, 1684
BmrFI	CCNGG	7 cut(s)	155, 332, 469, 521, 543, 904, 1302
BmrI	ACTGGG	1 cut(s)	198
BmsI	GCATC	3 cut(s)	697, 994, 1686
BmuI	ACTGGG	1 cut(s)	198
BpiI	GAAGAC	1 cut(s)	90
Bpu10I	CCTNAGC	1 cut(s)	324
Bpu1102I	GCTNAGC	2 cut(s)	225, 548
Bpu14I	TTCGAA	1 cut(s)	434
BpuEI	CTTGAG	1 cut(s)	857
BpuMI	CCSGG	3 cut(s)	332, 469, 1302
Bsa29I	ATCGAT	1 cut(s)	270
BsaAI	YACGTR	2 cut(s)	175, 1627
BsaHI	GRCGYC	3 cut(s)	5, 107, 113
BsaI	GGTCTC	1 cut(s)	437
BsaJI	CCNNGG	6 cut(s)	102, 541, 542, 1091, 1329, 1422
BsaWI	WCCGGW	1 cut(s)	462
Bsc4I	CCNNNNNNNGG	7 cut(s)	220, 337, 409, 909, 1093, 1555, 1692
Bse118I	RCCGGY	3 cut(s)	115, 462, 1542
Bse1I	ACTGG	1 cut(s)	193
Bse3DI	GCAATG	5 cut(s)	683, 728, 785, 996, 1377
BseBI	CCWGG	4 cut(s)	155, 521, 543, 904
BseCI	ATCGAT	1 cut(s)	270
BseDI	CCNNGG	6 cut(s)	102, 541, 542, 1091, 1329, 1422
BseGI	GGATG	3 cut(s)	153, 1102, 1701
BseLI	CCNNNNNNNGG	7 cut(s)	220, 337, 409, 909, 1093, 1555, 1692
BseMI	GCAATG	5 cut(s)	683, 728, 785, 996, 1377
BseMII	CTCAG	6 cut(s)	239, 315, 1044, 1133, 1190, 1254
BseNI	ACTGG	1 cut(s)	193
BsePI	GCGCGC	1 cut(s)	229
BseRI	GAGGAG	3 cut(s)	932, 1513, 1690
BseSI	GKGCMC	2 cut(s)	402, 1302
BseXI	GCAGC	6 cut(s)	475, 478, 605, 682, 849, 1585
BsgI	GTGCAG	3 cut(s)	42, 1083, 1483
Bsh1236I	CGCG	3 cut(s)	71, 231, 233
BshFI	GGCC	3 cut(s)	1520, 1542, 1559
BshNI	GGYRCC	4 cut(s)	112, 399, 1545, 1610
BshTI	ACCGGT	1 cut(s)	462
BshVI	ATCGAT	1 cut(s)	270
BsiHKAI	GWGCWC	4 cut(s)	213, 363, 528, 1346
BsiSI	CCGG	6 cut(s)	116, 331, 463, 468, 1302, 1543
BslFI	GGGAC	4 cut(s)	301, 820, 989, 1336
BslI	CCNNNNNNNGG	7 cut(s)	220, 337, 409, 909, 1093, 1555, 1692
BsmAI	GTCTC	5 cut(s)	27, 96, 362, 437, 1430
BsmBI	CGTCTC	2 cut(s)	96, 362
BsmFI	GGGAC	4 cut(s)	301, 820, 989, 1336
BsmI	GAATGC	2 cut(s)	685, 1511
BsnI	GGCC	3 cut(s)	1520, 1542, 1559
Bso31I	GGTCTC	1 cut(s)	437
Bsp119I	TTCGAA	1 cut(s)	434
Bsp1286I	GDGCHC	6 cut(s)	213, 363, 402, 528, 1302, 1346
Bsp143I	GATC	5 cut(s)	235, 475, 532, 742, 1122
Bsp1720I	GCTNAGC	2 cut(s)	225, 548
BspANI	GGCC	3 cut(s)	1520, 1542, 1559
BspCNI	CTCAG	6 cut(s)	238, 316, 1043, 1132, 1191, 1253
BspDI	ATCGAT	1 cut(s)	270
BspFNI	CGCG	3 cut(s)	71, 231, 233
BspHI	TCATGA	1 cut(s)	535
BspLI	GGNNCC	8 cut(s)	48, 75, 114, 401, 744, 1547, 1612, 1684
BspMAI	CTGCAG	1 cut(s)	595
BspMI	ACCTGC	1 cut(s)	172
BspPI	GGATC	4 cut(s)	243, 470, 737, 750
BspT104I	TTCGAA	1 cut(s)	434
BspT107I	GGYRCC	4 cut(s)	112, 399, 1545, 1610
BspTNI	GGTCTC	1 cut(s)	437
BsrBI	CCGCTC	1 cut(s)	1680
BsrDI	GCAATG	5 cut(s)	683, 728, 785, 996, 1377
BsrFI	RCCGGY	3 cut(s)	115, 462, 1542
BsrI	ACTGG	1 cut(s)	193
BssAI	RCCGGY	3 cut(s)	115, 462, 1542
BssECI	CCNNGG	6 cut(s)	102, 541, 542, 1091, 1329, 1422
BssHII	GCGCGC	1 cut(s)	229
BssMI	GATC	5 cut(s)	235, 475, 532, 742, 1122
BssNI	GRCGYC	3 cut(s)	5, 107, 113
BssSI	CACGAG	1 cut(s)	1690
BssT1I	CCWWGG	2 cut(s)	1091, 1422
Bst2BI	CACGAG	1 cut(s)	1690
Bst2UI	CCWGG	4 cut(s)	155, 521, 543, 904
Bst4CI	ACNGT	9 cut(s)	292, 448, 750, 815, 1114, 1249, 1273, 1297, 1576
Bst6I	CTCTTC	2 cut(s)	902, 1667
BstACI	GRCGYC	3 cut(s)	5, 107, 113
BstAPI	GCANNNNNTGC	1 cut(s)	1274
BstBAI	YACGTR	2 cut(s)	175, 1627
BstBI	TTCGAA	1 cut(s)	434
BstC8I	GCNNGC	7 cut(s)	117, 231, 486, 915, 1398, 1488, 1544
BstDEI	CTNAG	9 cut(s)	37, 225, 324, 548, 738, 1030, 1119, 1199, 1240
BstDSI	CCRYGG	2 cut(s)	102, 1329
BstF5I	GGATG	3 cut(s)	153, 1102, 1701
BstFNI	CGCG	3 cut(s)	71, 231, 233
BstH2I	RGCGCY	1 cut(s)	116
BstHHI	GCGC	4 cut(s)	115, 231, 233, 728
BstKTI	GATC	5 cut(s)	238, 478, 535, 745, 1125
BstMAI	GTCTC	5 cut(s)	27, 96, 362, 437, 1430
BstMBI	GATC	5 cut(s)	235, 475, 532, 742, 1122
BstMWI	GCNNNNNNNGC	7 cut(s)	230, 239, 848, 1070, 1274, 1283, 1517
BstNI	CCWGG	4 cut(s)	155, 521, 543, 904
BstNSI	RCATGY	1 cut(s)	488
BstSCI	CCNGG	7 cut(s)	153, 330, 467, 519, 541, 902, 1300
BstSFI	CTRYAG	1 cut(s)	591
BstSLI	GKGCMC	2 cut(s)	402, 1302
BstSNI	TACGTA	1 cut(s)	1627
BstUI	CGCG	3 cut(s)	71, 231, 233
BstV1I	GCAGC	6 cut(s)	475, 478, 605, 682, 849, 1585
BstV2I	GAAGAC	1 cut(s)	90
BstX2I	RGATCY	2 cut(s)	235, 742
BstYI	RGATCY	2 cut(s)	235, 742
Bsu15I	ATCGAT	1 cut(s)	270
BsuI	GTATCC	1 cut(s)	1638
BsuRI	GGCC	3 cut(s)	1520, 1542, 1559
BsuTUI	ATCGAT	1 cut(s)	270
BtgI	CCRYGG	2 cut(s)	102, 1329
BtgZI	GCGATG	1 cut(s)	63
BtsCI	GGATG	3 cut(s)	153, 1102, 1701
BtsIMutI	CAGTG	4 cut(s)	186, 965, 1245, 1512
BveI	ACCTGC	1 cut(s)	172
Cac8I	GCNNGC	7 cut(s)	117, 231, 486, 915, 1398, 1488, 1544
CaiI	CAGNNNCTG	1 cut(s)	1247
CciI	TCATGA	1 cut(s)	535
CfoI	GCGC	4 cut(s)	115, 231, 233, 728
Cfr10I	RCCGGY	3 cut(s)	115, 462, 1542
Cfr13I	GGNCC	6 cut(s)	47, 151, 328, 465, 563, 1557
ClaI	ATCGAT	1 cut(s)	270
CpoI	CGGWCCG	1 cut(s)	465
CseI	GACGC	1 cut(s)	96
Csp6I	GTAC	1 cut(s)	172
CspAI	ACCGGT	1 cut(s)	462
CspI	CGGWCCG	1 cut(s)	465
CviAII	CATG	6 cut(s)	485, 509, 536, 1516, 1615, 1654
CviQI	GTAC	1 cut(s)	172
DdeI	CTNAG	9 cut(s)	37, 225, 324, 548, 738, 1030, 1119, 1199, 1240
DinI	GGCGCC	1 cut(s)	114
DpnI	GATC	5 cut(s)	237, 477, 534, 744, 1124
DpnII	GATC	5 cut(s)	235, 475, 532, 742, 1122
EaeI	YGGCCR	1 cut(s)	1540
Eam1104I	CTCTTC	2 cut(s)	902, 1667
EarI	CTCTTC	2 cut(s)	902, 1667
EciI	GGCGGA	1 cut(s)	404
Ecl136II	GAGCTC	2 cut(s)	211, 361
Eco105I	TACGTA	1 cut(s)	1627
Eco130I	CCWWGG	2 cut(s)	1091, 1422
Eco24I	GRGCYC	2 cut(s)	213, 363
Eco31I	GGTCTC	1 cut(s)	437
Eco32I	GATATC	1 cut(s)	268
Eco47I	GGWCC	5 cut(s)	47, 151, 328, 465, 563
Eco53kI	GAGCTC	2 cut(s)	211, 361
Eco57I	CTGAAG	1 cut(s)	1488
EcoICRI	GAGCTC	2 cut(s)	211, 361
EcoRI	GAATTC	1 cut(s)	571
EcoRII	CCWGG	4 cut(s)	153, 519, 541, 902
EcoRV	GATATC	1 cut(s)	268
EcoT14I	CCWWGG	2 cut(s)	1091, 1422
EcoT22I	ATGCAT	1 cut(s)	685
EcoT38I	GRGCYC	2 cut(s)	213, 363
EgeI	GGCGCC	1 cut(s)	114
EheI	GGCGCC	1 cut(s)	114
ErhI	CCWWGG	2 cut(s)	1091, 1422
Esp3I	CGTCTC	2 cut(s)	96, 362
FaeI	CATG	6 cut(s)	488, 512, 539, 1519, 1618, 1657
FalI	AAGNNNNNCTT	2 cut(s)	387, 419
FaqI	GGGAC	4 cut(s)	301, 820, 989, 1336
FatI	CATG	6 cut(s)	484, 508, 535, 1515, 1614, 1653
FauI	CCCGC	2 cut(s)	151, 856
FauNDI	CATATG	1 cut(s)	792
FokI	GGATG	3 cut(s)	160, 1109, 1708
FriOI	GRGCYC	2 cut(s)	213, 363
FspBI	CTAG	2 cut(s)	239, 530
GlaI	GCGC	4 cut(s)	114, 230, 232, 727
HaeII	RGCGCY	1 cut(s)	116
HaeIII	GGCC	3 cut(s)	1520, 1542, 1559
HapII	CCGG	6 cut(s)	116, 331, 463, 468, 1302, 1543
HgaI	GACGC	1 cut(s)	96
HhaI	GCGC	4 cut(s)	115, 231, 233, 728
Hin1I	GRCGYC	3 cut(s)	5, 107, 113
Hin1II	CATG	6 cut(s)	488, 512, 539, 1519, 1618, 1657
Hin6I	GCGC	4 cut(s)	113, 229, 231, 726
HinP1I	GCGC	4 cut(s)	113, 229, 231, 726
HinfI	GANTC	7 cut(s)	256, 272, 436, 641, 1025, 1117, 1221
HpaII	CCGG	6 cut(s)	116, 331, 463, 468, 1302, 1543
HphI	GGTGA	5 cut(s)	38, 355, 835, 1126, 1319
Hpy166II	GTNNAC	2 cut(s)	381, 1180
Hpy188I	TCNGA	8 cut(s)	51, 365, 1021, 1033, 1122, 1430, 1705, 1720
Hpy188III	TCNNGA	7 cut(s)	138, 440, 536, 634, 836, 992, 1126
Hpy8I	GTNNAC	2 cut(s)	381, 1180
Hpy99I	CGWCG	4 cut(s)	88, 112, 571, 1396
HpyAV	CCTTC	2 cut(s)	37, 1211
HpyCH4III	ACNGT	9 cut(s)	292, 448, 750, 815, 1114, 1249, 1273, 1297, 1576
HpyCH4IV	ACGT	4 cut(s)	174, 1391, 1626, 1648
HpyF10VI	GCNNNNNNNGC	7 cut(s)	230, 239, 848, 1070, 1274, 1283, 1517
HpyF3I	CTNAG	9 cut(s)	37, 225, 324, 548, 738, 1030, 1119, 1199, 1240
HpySE526I	ACGT	4 cut(s)	174, 1391, 1626, 1648
Hsp92I	GRCGYC	3 cut(s)	5, 107, 113
Hsp92II	CATG	6 cut(s)	488, 512, 539, 1519, 1618, 1657
HspAI	GCGC	4 cut(s)	113, 229, 231, 726
KasI	GGCGCC	1 cut(s)	112
KroI	GCCGGC	2 cut(s)	115, 1542
KroNI	GCCGGC	2 cut(s)	117, 1544
Kzo9I	GATC	5 cut(s)	235, 475, 532, 742, 1122
LmnI	GCTCC	6 cut(s)	208, 216, 418, 531, 1212, 1677
Lsp1109I	GCAGC	6 cut(s)	475, 478, 605, 682, 849, 1585
LweI	GCATC	3 cut(s)	697, 994, 1686
MaeI	CTAG	2 cut(s)	239, 530
MaeII	ACGT	4 cut(s)	174, 1391, 1626, 1648
MaeIII	GTNAC	7 cut(s)	184, 448, 878, 936, 1114, 1243, 1570
MalI	GATC	5 cut(s)	237, 477, 534, 744, 1124
MbiI	CCGCTC	1 cut(s)	1680
MboI	GATC	5 cut(s)	235, 475, 532, 742, 1122
MfeI	CAATTG	2 cut(s)	558, 852
MflI	RGATCY	2 cut(s)	235, 742
MhlI	GDGCHC	6 cut(s)	213, 363, 402, 528, 1302, 1346
MluCI	AATT	9 cut(s)	558, 571, 827, 852, 872, 1100, 1416, 1714, 1722
Mly113I	GGCGCC	1 cut(s)	113
MlyI	GAGTC	1 cut(s)	1111
MmeI	TCCRAC	3 cut(s)	156, 633, 1710
Mph1103I	ATGCAT	1 cut(s)	685
MroNI	GCCGGC	2 cut(s)	115, 1542
MseI	TTAA	3 cut(s)	414, 960, 1338
MslI	CAYNNNNRTG	3 cut(s)	405, 1250, 1505
MspI	CCGG	6 cut(s)	116, 331, 463, 468, 1302, 1543
MspR9I	CCNGG	7 cut(s)	155, 332, 469, 521, 543, 904, 1302
MunI	CAATTG	2 cut(s)	558, 852
Mva1269I	GAATGC	2 cut(s)	685, 1511
MvaI	CCWGG	4 cut(s)	155, 521, 543, 904
MvnI	CGCG	3 cut(s)	71, 231, 233
MwoI	GCNNNNNNNGC	7 cut(s)	230, 239, 848, 1070, 1274, 1283, 1517
NaeI	GCCGGC	2 cut(s)	117, 1544
NarI	GGCGCC	1 cut(s)	113
NciI	CCSGG	3 cut(s)	332, 469, 1302
NdeI	CATATG	1 cut(s)	792
NdeII	GATC	5 cut(s)	235, 475, 532, 742, 1122
NgoMIV	GCCGGC	2 cut(s)	115, 1542
NlaIII	CATG	6 cut(s)	488, 512, 539, 1519, 1618, 1657
NlaIV	GGNNCC	8 cut(s)	48, 75, 114, 401, 744, 1547, 1612, 1684
NmeAIII	GCCGAG	2 cut(s)	240, 1587
NmuCI	GTSAC	3 cut(s)	184, 1114, 1243
NsiI	ATGCAT	1 cut(s)	685
NspI	RCATGY	1 cut(s)	488
NspV	TTCGAA	1 cut(s)	434
OliI	CACNNNNGTG	2 cut(s)	1250, 1505
PaeI	GCATGC	1 cut(s)	488
PagI	TCATGA	1 cut(s)	535
PasI	CCCWGGG	1 cut(s)	542
PauI	GCGCGC	1 cut(s)	229
PcsI	WCGNNNNNNNCGW	4 cut(s)	86, 92, 267, 1326
PctI	GAATGC	2 cut(s)	685, 1511
PdiI	GCCGGC	2 cut(s)	117, 1544
PfeI	GAWTC	6 cut(s)	256, 272, 436, 641, 1025, 1221
PflMI	CCANNNNNTGG	1 cut(s)	1692
PinAI	ACCGGT	1 cut(s)	462
PleI	GAGTC	1 cut(s)	1111
PluTI	GGCGCC	1 cut(s)	116
PpsI	GAGTC	1 cut(s)	1111
Ppu21I	YACGTR	2 cut(s)	175, 1627
Psp124BI	GAGCTC	2 cut(s)	213, 363
Psp6I	CCWGG	4 cut(s)	153, 519, 541, 902
PspGI	CCWGG	4 cut(s)	153, 519, 541, 902
PspN4I	GGNNCC	8 cut(s)	48, 75, 114, 401, 744, 1547, 1612, 1684
PspPI	GGNCC	6 cut(s)	47, 151, 328, 465, 563, 1557
PstI	CTGCAG	1 cut(s)	595
PstNI	CAGNNNCTG	1 cut(s)	1247
PsuI	RGATCY	2 cut(s)	235, 742
PteI	GCGCGC	1 cut(s)	229
RsaI	GTAC	1 cut(s)	173
RsaNI	GTAC	1 cut(s)	172
RseI	CAYNNNNRTG	3 cut(s)	405, 1250, 1505
Rsr2I	CGGWCCG	1 cut(s)	465
RsrII	CGGWCCG	1 cut(s)	465
SacI	GAGCTC	2 cut(s)	213, 363
SaqAI	TTAA	3 cut(s)	414, 960, 1338
Sau3AI	GATC	5 cut(s)	235, 475, 532, 742, 1122
Sau96I	GGNCC	6 cut(s)	47, 151, 328, 465, 563, 1557
SchI	GAGTC	1 cut(s)	1111
ScrFI	CCNGG	7 cut(s)	155, 332, 469, 521, 543, 904, 1302
SduI	GDGCHC	6 cut(s)	213, 363, 402, 528, 1302, 1346
SfaNI	GCATC	3 cut(s)	697, 994, 1686
SfcI	CTRYAG	1 cut(s)	591
SfoI	GGCGCC	1 cut(s)	114
SfuI	TTCGAA	1 cut(s)	434
SinI	GGWCC	5 cut(s)	47, 151, 328, 465, 563
SmiMI	CAYNNNNRTG	3 cut(s)	405, 1250, 1505
SmlI	CTYRAG	1 cut(s)	836
SmoI	CTYRAG	1 cut(s)	836
SnaBI	TACGTA	1 cut(s)	1627
SphI	GCATGC	1 cut(s)	488
Sse9I	AATT	9 cut(s)	558, 571, 827, 852, 872, 1100, 1416, 1714, 1722
SspDI	GGCGCC	1 cut(s)	112
SspI	AATATT	1 cut(s)	431
SspMI	CTAG	2 cut(s)	239, 530
SstI	GAGCTC	2 cut(s)	213, 363
StyD4I	CCNGG	7 cut(s)	153, 330, 467, 519, 541, 902, 1300
StyI	CCWWGG	2 cut(s)	1091, 1422
TaaI	ACNGT	9 cut(s)	292, 448, 750, 815, 1114, 1249, 1273, 1297, 1576
TaiI	ACGT	4 cut(s)	177, 1394, 1629, 1651
TaqI	TCGA	6 cut(s)	15, 270, 434, 569, 635, 1637
TasI	AATT	9 cut(s)	558, 571, 827, 852, 872, 1100, 1416, 1714, 1722
TauI	GCSGC	7 cut(s)	196, 224, 245, 338, 389, 1286, 1562
TfiI	GAWTC	6 cut(s)	256, 272, 436, 641, 1025, 1221
Tru1I	TTAA	3 cut(s)	414, 960, 1338
Tru9I	TTAA	3 cut(s)	414, 960, 1338
TscAI	CASTG	4 cut(s)	193, 972, 1252, 1512
TseFI	GTSAC	3 cut(s)	184, 1114, 1243
TseI	GCWGC	6 cut(s)	488, 491, 593, 695, 862, 1598
Tsp45I	GTSAC	3 cut(s)	184, 1114, 1243
TspDTI	ATGAA	6 cut(s)	639, 1092, 1113, 1127, 1354, 1504
TspGWI	ACGGA	5 cut(s)	84, 221, 329, 555, 1744
TspRI	CASTG	4 cut(s)	193, 972, 1252, 1512
Van91I	CCANNNNNTGG	1 cut(s)	1692
VpaK11BI	GGWCC	5 cut(s)	47, 151, 328, 465, 563
XapI	RAATTY	3 cut(s)	571, 872, 1714
XceI	RCATGY	1 cut(s)	488
XspI	CTAG	2 cut(s)	239, 530
Zsp2I	ATGCAT	1 cut(s)	685

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.