Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0010764

Physical Location & Seq

Genomic Coordinates Forward (+)

11650058 .. 11653865

Gene Structure 3808 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000027438

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1707 bp

ATGTTGACGACTCCAGCGGACCCGTCGCCGTCGCCGTCTCCGTCATCGACGGCATCGGCGCCGGCGCTGGTGCTGTCGAACTCCGGGAAGAGGATGGACCAGGCGGGGAAGAAGAAGTATGTGAAGCAGGTGACCGGGCGGCACAACGACACCGAGCTCCACCTGGCGGCGCAGAAGGGCGATCTGGCCGCCGTGAAGCAGATTCTGCGTGATATTGTTTCGCAGGTGGAGGGGACGGTGAGTGGGGCTGAGTTGGATTCGGAGGTGGCGGAGGTGAGGGCGGCGGTGGTGAATGATGTGAATGAGCTGGGGGAGACGGCGTTGTTCACGGCGGCGGATAGAGGGCATCTGGATGTGGTGAAGGAGTTGTTGCAGTATTCAAATAAGGAGAGTGTTGTGAAGAAGAATCGGTCCGGGTTTAGTCCCTTGCATATTGCTGCAACTCAAGGCCACCATGCCATTGTTCAGGTACTCCTGGATCATGACCCTGGGCTAAGCAGGACAATTGGCCCATCGAATGCAACCCCACTTATATCTGCAGCTCAAAGAGGGCATATAGCTGTGGTTGATGAGCTGTTGTCAAAGGATTCTACCTTGTTGGAGATTTCAAGATCAAATGGGAAGAATGCATTGCATCTGGCTGCAAGACCAGGGCATGTGGAGATTGTAAGAGCATTGCTAAATAGGGATCCACAATTGGCACGAAGGACTGACAAGAAAGGGCAAACTGCCTTGCATATGGCTGTTAAAGGGACAAACTGTGAGGTGGTGAAATTGCTACTGGATGCAGATGCTGCAATTGTGATGCTTCCTGACAAATTCGGTAACACAGCATTACATGTAGCCACCAGGAAGAAGCGAGCTGAGATAGTGAACGAGTTGTTATCCCTCCCAGACAGCAATGTTAATGCACTGAATAGGGACCACAAAACCGCTCTTGACATTGCTGAAGAGCTTCCACTTTCGGAAGAATCAACAGAAGTAAGGGCTTGCCTTTACAAATACGGTGCTGTTAGAGCTAATGAACTGAATCAACCAAGGGATGAACTGAGAAAAACAGTGACTCAGATCAAGAATGATGTTCATATCCAGCTTGAACAAACAAAGAAAACCAATAAAAATGTTCAGAATATTTCGAAAGACCTCAGGAAGCTCCATAGAGAAGGAATCAACAATGCCACCAACTCAGTCACTGTGGTGGCTGTTCTATTTGCAACAGTTGCTTTTGCAGCTATATTTACTGTGCCCGGTGGTGATAACGACGATGGGACGGCTGTGGTAGTGCACTCTTCTCCATTCAAAATCTTCTTCATTTTTAATGCCATTGCTCTTTTTACTTCTCTGGCCGTTGTTGTGGTTCAAATTACATTGGTCAGAGGCGAGACAAAAGCAGAGAAAAAGGTCGTAGTAATTATCAACAAGTTGATGTGGCTCGCGTCAGTGTGCACTTCAGTGGCATTCATGGCCTCCTCTTACATTGTGGTTGGCCGGCATCATAAGTGGGCTGCAATTCTGGTAACAGTTGTTGGGGGAATGATAATGGCAGCGGTTCTTGGCACCATGACATATTGTGTGGTGAAGTCAAAGCGGCACCGGGCCTTGAGAAAGAAGGATAAGAGCGCAAGGAGAAATGGACTTAGTGGATCCACCTCGTGGATGCCCTCAGACTACTCCAATTCAGAAATTGATCGAATCTATGCCCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005886 GO:0006950 GO:0006970 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0009628 GO:0009651 GO:0009987 GO:0016020 GO:0023052 GO:0033036 GO:0034613 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051641 GO:0051716 GO:0065007 GO:0070727 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank Ank_2 Ank_4 PGG

Protein Analysis

569

Amino Acids

61.57

Weight (kDa)

9.42

Isoelectric Point (pI)

36.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ank_2	PF12796	53 - 162	6.6e-16	Ankyrin repeats (3 copies)
Ank_KRIT1	PF24521	104 - 196	4.1e-07	KRIT1 ankyrin-repeats domain
Ank_4	PF13637	108 - 159	5.6e-08	Ankyrin repeats (many copies)
Ank_2	PF12796	172 - 230	1.9e-10	Ankyrin repeats (3 copies)
Ank_4	PF13637	211 - 261	2.3e-10	Ankyrin repeats (many copies)
Ank_5	PF13857	226 - 264	6.1e-06	Ankyrin repeats (many copies)
Ank_2	PF12796	237 - 303	1e-11	Ankyrin repeats (3 copies)
Ank_5	PF13857	260 - 316	4.6e-07	Ankyrin repeats (many copies)
PGG	PF13962	388 - 494	6.9e-29	Domain of unknown function

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010764)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G12360 AT3G12360
fragaria_vesca	FvH4_7g20900
malus_domestica	MD01G1118500.v1.1 MD07G1186700.v1.1
prunus_persica	Prupe.2G225600_v2.0.a1
pyrus_communis	pycom01g14570
rosa_chinensis	RchiOBHm_Chr1g0365211
rosa_laevigata	RLG00000027438
rosa_multiflora	Rmu_co8385283.1_g000001 Rmu_sc0008035.1_g000005 Rmu_sc0024070.1_g000002
rosa_roxburghii	Rroxscaffold_4G00291370 Rroxscaffold_4G00291910
rosa_rugosa	Rorug01G0320300
rosa_samantha	Rh1AG329300 Rh1BG290700 Rh1CG307600 Rh1DG322400
rosa_wichuraiana	Rw1G029200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	118, 214
Acc36I	ACCTGC	2 cut(s)	118, 214
AccB1I	GGYRCC	3 cut(s)	58, 1556, 1590
AccB7I	CCANNNNNTGG	1 cut(s)	1653
AccBSI	CCGCTC	1 cut(s)	935
AccII	CGCG	1 cut(s)	1436
AclWI	GGATC	5 cut(s)	486, 683, 696, 1638, 1651
AcoI	YGGCCR	3 cut(s)	186, 1344, 1486
AcsI	RAATTY	1 cut(s)	818
AcuI	CTGAAG	2 cut(s)	969, 1434
AcyI	GRCGYC	1 cut(s)	59
AdeI	CACNNNGTG	1 cut(s)	1653
AfaI	GTAC	1 cut(s)	471
AfiI	CCNNNNNNNGG	3 cut(s)	90, 166, 1653
AflIII	ACRYGT	1 cut(s)	838
AgsI	TTSAA	5 cut(s)	381, 609, 1097, 1300, 1361
AjnI	CCWGG	6 cut(s)	99, 162, 474, 487, 649, 848
AleI	CACNNNNGTG	2 cut(s)	1196, 1451
Alw21I	GWGCWC	3 cut(s)	159, 1287, 1448
Alw26I	GTCTC	3 cut(s)	42, 308, 1376
Alw44I	GTGCAC	2 cut(s)	1283, 1444
AlwI	GGATC	5 cut(s)	486, 683, 696, 1638, 1651
AlwNI	CAGNNNCTG	3 cut(s)	205, 794, 1193
AoxI	GGCC	7 cut(s)	186, 448, 508, 1344, 1464, 1486, 1596
ApaLI	GTGCAC	2 cut(s)	1283, 1444
ApeKI	GCWGC	7 cut(s)	437, 539, 641, 794, 1229, 1505, 1544
ApoI	RAATTY	1 cut(s)	818
Asp700I	GAANNNNTTC	1 cut(s)	954
AspLEI	GCGC	4 cut(s)	61, 67, 172, 1622
AspS9I	GGNCC	6 cut(s)	19, 97, 411, 509, 922, 1596
AsuC2I	CCSGG	5 cut(s)	85, 136, 415, 1248, 1595
AsuHPI	GGTGA	8 cut(s)	142, 250, 286, 301, 370, 781, 1265, 1588
AsuII	TTCGAA	1 cut(s)	1136
AvaII	GGWCC	4 cut(s)	19, 97, 411, 922
AxyI	CCTNAGG	1 cut(s)	1145
BaeGI	GKGCMC	3 cut(s)	1248, 1287, 1448
BamHI	GGATCC	2 cut(s)	688, 1643
BanI	GGYRCC	3 cut(s)	58, 1556, 1590
BanII	GRGCYC	1 cut(s)	159
BauI	CACGAG	1 cut(s)	1651
Bbv12I	GWGCWC	3 cut(s)	159, 1287, 1448
BbvI	GCAGC	7 cut(s)	424, 551, 628, 781, 1241, 1492, 1556
BccI	CCATC	3 cut(s)	88, 520, 1259
BceAI	ACGGC	8 cut(s)	13, 19, 66, 176, 333, 345, 1287, 1331
BciT130I	CCWGG	6 cut(s)	101, 164, 476, 489, 651, 850
BcnI	CCSGG	5 cut(s)	85, 136, 415, 1248, 1595
BcoDI	GTCTC	3 cut(s)	42, 308, 1376
BfmI	CTRYAG	1 cut(s)	537
BfoI	RGCGCY	2 cut(s)	62, 68
BfuAI	ACCTGC	2 cut(s)	118, 214
BlpI	GCTNAGC	1 cut(s)	494
Bme18I	GGWCC	4 cut(s)	19, 97, 411, 922
BmgT120I	GGNCC	6 cut(s)	19, 97, 411, 509, 922, 1596
BmiI	GGNNCC	7 cut(s)	21, 60, 690, 923, 1558, 1592, 1645
BmsI	GCATC	8 cut(s)	62, 355, 643, 775, 781, 795, 1501, 1647
Bpu1102I	GCTNAGC	1 cut(s)	494
Bpu14I	TTCGAA	1 cut(s)	1136
BpuEI	CTTGAG	2 cut(s)	429, 1621
BpuMI	CCSGG	5 cut(s)	85, 136, 415, 1248, 1595
BsaHI	GRCGYC	1 cut(s)	59
BsaJI	CCNNGG	4 cut(s)	487, 488, 650, 1037
BsaXI	ACNNNNNCTCC	2 cut(s)	305, 335
Bsc4I	CCNNNNNNNGG	3 cut(s)	90, 166, 1653
Bse118I	RCCGGY	2 cut(s)	61, 1488
Bse1I	ACTGG	1 cut(s)	786
Bse21I	CCTNAGG	1 cut(s)	1145
Bse3DI	GCAATG	5 cut(s)	629, 674, 907, 942, 1323
BseBI	CCWGG	6 cut(s)	101, 164, 476, 489, 651, 850
BseDI	CCNNGG	4 cut(s)	487, 488, 650, 1037
BseGI	GGATG	5 cut(s)	99, 358, 790, 1048, 1662
BseLI	CCNNNNNNNGG	3 cut(s)	90, 166, 1653
BseMI	GCAATG	5 cut(s)	629, 674, 907, 942, 1323
BseMII	CTCAG	7 cut(s)	240, 855, 1040, 1079, 1159, 1200, 1677
BseNI	ACTGG	1 cut(s)	786
BseRI	GAGGAG	1 cut(s)	1459
BseSI	GKGCMC	3 cut(s)	1248, 1287, 1448
BseXI	GCAGC	7 cut(s)	424, 551, 628, 781, 1241, 1492, 1556
BseYI	CCCAGC	1 cut(s)	307
Bsh1236I	CGCG	1 cut(s)	1436
BshFI	GGCC	7 cut(s)	188, 450, 510, 1346, 1466, 1488, 1598
BshNI	GGYRCC	3 cut(s)	58, 1556, 1590
BsiHKAI	GWGCWC	3 cut(s)	159, 1287, 1448
BsiSI	CCGG	7 cut(s)	62, 84, 135, 414, 1248, 1489, 1594
BslFI	GGGAC	5 cut(s)	247, 408, 766, 935, 1282
BslI	CCNNNNNNNGG	3 cut(s)	90, 166, 1653
BsmAI	GTCTC	3 cut(s)	42, 308, 1376
BsmBI	CGTCTC	2 cut(s)	42, 308
BsmFI	GGGAC	5 cut(s)	247, 408, 766, 935, 1282
BsmI	GAATGC	3 cut(s)	523, 631, 1457
BsnI	GGCC	7 cut(s)	188, 450, 510, 1346, 1466, 1488, 1598
Bsp119I	TTCGAA	1 cut(s)	1136
Bsp1286I	GDGCHC	4 cut(s)	159, 1248, 1287, 1448
Bsp143I	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
Bsp1720I	GCTNAGC	1 cut(s)	494
BspANI	GGCC	7 cut(s)	188, 450, 510, 1346, 1466, 1488, 1598
BspCNI	CTCAG	7 cut(s)	241, 856, 1041, 1078, 1158, 1199, 1676
BspFNI	CGCG	1 cut(s)	1436
BspHI	TCATGA	1 cut(s)	481
BspLI	GGNNCC	7 cut(s)	21, 60, 690, 923, 1558, 1592, 1645
BspMAI	CTGCAG	1 cut(s)	541
BspMI	ACCTGC	2 cut(s)	118, 214
BspPI	GGATC	5 cut(s)	486, 683, 696, 1638, 1651
BspQI	GCTCTTC	1 cut(s)	945
BspT104I	TTCGAA	1 cut(s)	1136
BspT107I	GGYRCC	3 cut(s)	58, 1556, 1590
BsrBI	CCGCTC	1 cut(s)	935
BsrDI	GCAATG	5 cut(s)	629, 674, 907, 942, 1323
BsrFI	RCCGGY	2 cut(s)	61, 1488
BsrI	ACTGG	1 cut(s)	786
BssAI	RCCGGY	2 cut(s)	61, 1488
BssECI	CCNNGG	4 cut(s)	487, 488, 650, 1037
BssMI	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	1 cut(s)	1651
BssT1I	CCWWGG	1 cut(s)	1037
Bst2BI	CACGAG	1 cut(s)	1651
Bst2UI	CCWGG	6 cut(s)	101, 164, 476, 489, 651, 850
Bst4CI	ACNGT	8 cut(s)	238, 761, 1007, 1060, 1195, 1219, 1243, 1522
Bst6I	CTCTTC	3 cut(s)	83, 945, 1294
BstACI	GRCGYC	1 cut(s)	59
BstAPI	GCANNNNNTGC	3 cut(s)	205, 794, 1220
BstBI	TTCGAA	1 cut(s)	1136
BstC8I	GCNNGC	5 cut(s)	63, 861, 991, 1434, 1490
BstDEI	CTNAG	9 cut(s)	249, 494, 864, 1049, 1065, 1145, 1186, 1637, 1663
BstEII	GGTNACC	1 cut(s)	130
BstF5I	GGATG	5 cut(s)	99, 358, 790, 1048, 1662
BstFNI	CGCG	1 cut(s)	1436
BstH2I	RGCGCY	2 cut(s)	62, 68
BstHHI	GCGC	4 cut(s)	61, 67, 172, 1622
BstKTI	GATC	7 cut(s)	184, 481, 614, 691, 1071, 1646, 1690
BstMAI	GTCTC	3 cut(s)	42, 308, 1376
BstMBI	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
BstMWI	GCNNNNNNNGC	6 cut(s)	205, 794, 1016, 1220, 1229, 1463
BstNI	CCWGG	6 cut(s)	101, 164, 476, 489, 651, 850
BstNSI	RCATGY	2 cut(s)	659, 842
BstPI	GGTNACC	1 cut(s)	130
BstSFI	CTRYAG	1 cut(s)	537
BstSLI	GKGCMC	3 cut(s)	1248, 1287, 1448
BstUI	CGCG	1 cut(s)	1436
BstV1I	GCAGC	7 cut(s)	424, 551, 628, 781, 1241, 1492, 1556
BstX2I	RGATCY	2 cut(s)	688, 1643
BstYI	RGATCY	2 cut(s)	688, 1643
Bsu36I	CCTNAGG	1 cut(s)	1145
BsuRI	GGCC	7 cut(s)	188, 450, 510, 1346, 1466, 1488, 1598
BtsCI	GGATG	5 cut(s)	99, 358, 790, 1048, 1662
BtsIMutI	CAGTG	5 cut(s)	911, 1065, 1191, 1446, 1458
BveI	ACCTGC	2 cut(s)	118, 214
Cac8I	GCNNGC	5 cut(s)	63, 861, 991, 1434, 1490
CaiI	CAGNNNCTG	3 cut(s)	205, 794, 1193
CciI	TCATGA	1 cut(s)	481
CfoI	GCGC	4 cut(s)	61, 67, 172, 1622
Cfr10I	RCCGGY	2 cut(s)	61, 1488
Cfr13I	GGNCC	6 cut(s)	19, 97, 411, 509, 922, 1596
CpoI	CGGWCCG	1 cut(s)	411
CseI	GACGC	1 cut(s)	1425
Csp6I	GTAC	1 cut(s)	470
CspI	CGGWCCG	1 cut(s)	411
CviAII	CATG	6 cut(s)	455, 482, 656, 839, 1462, 1561
CviQI	GTAC	1 cut(s)	470
DdeI	CTNAG	9 cut(s)	249, 494, 864, 1049, 1065, 1145, 1186, 1637, 1663
DinI	GGCGCC	1 cut(s)	60
DpnI	GATC	7 cut(s)	183, 480, 613, 690, 1070, 1645, 1689
DpnII	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
DraIII	CACNNNGTG	1 cut(s)	1653
EaeI	YGGCCR	3 cut(s)	186, 1344, 1486
Eam1104I	CTCTTC	3 cut(s)	83, 945, 1294
EarI	CTCTTC	3 cut(s)	83, 945, 1294
EciI	GGCGGA	2 cut(s)	284, 350
Ecl136II	GAGCTC	1 cut(s)	157
Eco130I	CCWWGG	1 cut(s)	1037
Eco24I	GRGCYC	1 cut(s)	159
Eco47I	GGWCC	4 cut(s)	19, 97, 411, 922
Eco53kI	GAGCTC	1 cut(s)	157
Eco57I	CTGAAG	2 cut(s)	969, 1434
Eco81I	CCTNAGG	1 cut(s)	1145
Eco91I	GGTNACC	1 cut(s)	130
EcoICRI	GAGCTC	1 cut(s)	157
EcoO65I	GGTNACC	1 cut(s)	130
EcoRII	CCWGG	6 cut(s)	99, 162, 474, 487, 649, 848
EcoT14I	CCWWGG	1 cut(s)	1037
EcoT22I	ATGCAT	1 cut(s)	631
EcoT38I	GRGCYC	1 cut(s)	159
EgeI	GGCGCC	1 cut(s)	60
EheI	GGCGCC	1 cut(s)	60
ErhI	CCWWGG	1 cut(s)	1037
Esp3I	CGTCTC	2 cut(s)	42, 308
FaeI	CATG	6 cut(s)	458, 485, 659, 842, 1465, 1564
FaqI	GGGAC	5 cut(s)	247, 408, 766, 935, 1282
FatI	CATG	6 cut(s)	454, 481, 655, 838, 1461, 1560
FauI	CCCGC	1 cut(s)	97
FauNDI	CATATG	1 cut(s)	738
FokI	GGATG	5 cut(s)	106, 365, 797, 1055, 1669
FriOI	GRGCYC	1 cut(s)	159
GlaI	GCGC	4 cut(s)	60, 66, 171, 1621
GsaI	CCCAGC	1 cut(s)	311
HaeII	RGCGCY	2 cut(s)	62, 68
HaeIII	GGCC	7 cut(s)	188, 450, 510, 1346, 1466, 1488, 1598
HapII	CCGG	7 cut(s)	62, 84, 135, 414, 1248, 1489, 1594
HgaI	GACGC	1 cut(s)	1425
HhaI	GCGC	4 cut(s)	61, 67, 172, 1622
Hin1I	GRCGYC	1 cut(s)	59
Hin1II	CATG	6 cut(s)	458, 485, 659, 842, 1465, 1564
Hin6I	GCGC	4 cut(s)	59, 65, 170, 1620
HinP1I	GCGC	4 cut(s)	59, 65, 170, 1620
HincII	GTYRAC	1 cut(s)	6
HindII	GTYRAC	1 cut(s)	6
HpaII	CCGG	7 cut(s)	62, 84, 135, 414, 1248, 1489, 1594
HphI	GGTGA	8 cut(s)	142, 250, 286, 301, 370, 781, 1265, 1588
Hpy166II	GTNNAC	5 cut(s)	6, 327, 874, 1285, 1446
Hpy188I	TCNGA	7 cut(s)	262, 967, 1068, 1128, 1376, 1666, 1681
Hpy188III	TCNNGA	7 cut(s)	350, 482, 609, 812, 938, 1072, 1147
Hpy8I	GTNNAC	5 cut(s)	6, 327, 874, 1285, 1446
Hpy99I	CGWCG	4 cut(s)	28, 34, 52, 1265
HpyAV	CCTTC	5 cut(s)	169, 355, 699, 1157, 1603
HpyCH4III	ACNGT	8 cut(s)	238, 761, 1007, 1060, 1195, 1219, 1243, 1522
HpyF10VI	GCNNNNNNNGC	6 cut(s)	205, 794, 1016, 1220, 1229, 1463
HpyF3I	CTNAG	9 cut(s)	249, 494, 864, 1049, 1065, 1145, 1186, 1637, 1663
Hsp92I	GRCGYC	1 cut(s)	59
Hsp92II	CATG	6 cut(s)	458, 485, 659, 842, 1465, 1564
HspAI	GCGC	4 cut(s)	59, 65, 170, 1620
KasI	GGCGCC	1 cut(s)	58
KroI	GCCGGC	2 cut(s)	61, 1488
KroNI	GCCGGC	2 cut(s)	63, 1490
Kzo9I	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
LguI	GCTCTTC	1 cut(s)	945
LmnI	GCTCC	2 cut(s)	162, 1158
Lsp1109I	GCAGC	7 cut(s)	424, 551, 628, 781, 1241, 1492, 1556
LweI	GCATC	8 cut(s)	62, 355, 643, 775, 781, 795, 1501, 1647
MaeIII	GTNAC	5 cut(s)	130, 824, 1060, 1189, 1516
MalI	GATC	7 cut(s)	183, 480, 613, 690, 1070, 1645, 1689
MbiI	CCGCTC	1 cut(s)	935
MboI	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
MfeI	CAATTG	3 cut(s)	504, 695, 798
MflI	RGATCY	2 cut(s)	688, 1643
MhlI	GDGCHC	4 cut(s)	159, 1248, 1287, 1448
Mly113I	GGCGCC	1 cut(s)	59
MlyI	GAGTC	2 cut(s)	4, 1057
MmeI	TCCRAC	2 cut(s)	234, 579
Mph1103I	ATGCAT	1 cut(s)	631
MreI	CGCCGGCG	1 cut(s)	61
MroNI	GCCGGC	2 cut(s)	61, 1488
MroXI	GAANNNNTTC	1 cut(s)	954
MseI	TTAA	3 cut(s)	747, 906, 1317
MslI	CAYNNNNRTG	3 cut(s)	351, 1196, 1451
MspA1I	CMGCKG	2 cut(s)	17, 1547
MspI	CCGG	7 cut(s)	62, 84, 135, 414, 1248, 1489, 1594
MunI	CAATTG	3 cut(s)	504, 695, 798
Mva1269I	GAATGC	3 cut(s)	523, 631, 1457
MvaI	CCWGG	6 cut(s)	101, 164, 476, 489, 651, 850
MvnI	CGCG	1 cut(s)	1436
MwoI	GCNNNNNNNGC	6 cut(s)	205, 794, 1016, 1220, 1229, 1463
NaeI	GCCGGC	2 cut(s)	63, 1490
NarI	GGCGCC	1 cut(s)	59
NciI	CCSGG	5 cut(s)	85, 136, 415, 1248, 1595
NdeI	CATATG	1 cut(s)	738
NdeII	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
NgoMIV	GCCGGC	2 cut(s)	61, 1488
NlaIII	CATG	6 cut(s)	458, 485, 659, 842, 1465, 1564
NlaIV	GGNNCC	7 cut(s)	21, 60, 690, 923, 1558, 1592, 1645
NmuCI	GTSAC	3 cut(s)	130, 1060, 1189
NsiI	ATGCAT	1 cut(s)	631
NspI	RCATGY	2 cut(s)	659, 842
NspV	TTCGAA	1 cut(s)	1136
OliI	CACNNNNGTG	2 cut(s)	1196, 1451
PagI	TCATGA	1 cut(s)	481
PaqCI	CACCTGC	2 cut(s)	118, 214
PasI	CCCWGGG	1 cut(s)	488
PciI	ACATGT	1 cut(s)	838
PciSI	GCTCTTC	1 cut(s)	945
PcsI	WCGNNNNNNNCGW	2 cut(s)	32, 38
PctI	GAATGC	3 cut(s)	523, 631, 1457
PdiI	GCCGGC	2 cut(s)	63, 1490
PdmI	GAANNNNTTC	1 cut(s)	954
PfeI	GAWTC	8 cut(s)	202, 257, 406, 587, 971, 1030, 1167, 1692
PflMI	CCANNNNNTGG	1 cut(s)	1653
PfoI	TCCNGGA	2 cut(s)	83, 474
PleI	GAGTC	2 cut(s)	4, 1057
PluTI	GGCGCC	1 cut(s)	62
PpsI	GAGTC	2 cut(s)	4, 1057
PscI	ACATGT	1 cut(s)	838
Psp124BI	GAGCTC	1 cut(s)	159
Psp6I	CCWGG	6 cut(s)	99, 162, 474, 487, 649, 848
PspEI	GGTNACC	1 cut(s)	130
PspFI	CCCAGC	1 cut(s)	307
PspGI	CCWGG	6 cut(s)	99, 162, 474, 487, 649, 848
PspN4I	GGNNCC	7 cut(s)	21, 60, 690, 923, 1558, 1592, 1645
PspPI	GGNCC	6 cut(s)	19, 97, 411, 509, 922, 1596
PstI	CTGCAG	1 cut(s)	541
PstNI	CAGNNNCTG	3 cut(s)	205, 794, 1193
PsuI	RGATCY	2 cut(s)	688, 1643
RsaI	GTAC	1 cut(s)	471
RsaNI	GTAC	1 cut(s)	470
RseI	CAYNNNNRTG	3 cut(s)	351, 1196, 1451
Rsr2I	CGGWCCG	1 cut(s)	411
RsrII	CGGWCCG	1 cut(s)	411
SacI	GAGCTC	1 cut(s)	159
SapI	GCTCTTC	1 cut(s)	945
SaqAI	TTAA	3 cut(s)	747, 906, 1317
Sau3AI	GATC	7 cut(s)	181, 478, 611, 688, 1068, 1643, 1687
Sau96I	GGNCC	6 cut(s)	19, 97, 411, 509, 922, 1596
SchI	GAGTC	2 cut(s)	4, 1057
SduI	GDGCHC	4 cut(s)	159, 1248, 1287, 1448
SfaNI	GCATC	8 cut(s)	62, 355, 643, 775, 781, 795, 1501, 1647
SfcI	CTRYAG	1 cut(s)	537
SfoI	GGCGCC	1 cut(s)	60
SfuI	TTCGAA	1 cut(s)	1136
SgrAI	CRCCGGYG	1 cut(s)	61
SinI	GGWCC	4 cut(s)	19, 97, 411, 922
SmiMI	CAYNNNNRTG	3 cut(s)	351, 1196, 1451
SmlI	CTYRAG	2 cut(s)	444, 1600
SmoI	CTYRAG	2 cut(s)	444, 1600
SspDI	GGCGCC	1 cut(s)	58
SspI	AATATT	1 cut(s)	1132
SstI	GAGCTC	1 cut(s)	159
StyI	CCWWGG	1 cut(s)	1037
TaaI	ACNGT	8 cut(s)	238, 761, 1007, 1060, 1195, 1219, 1243, 1522
TaqI	TCGA	5 cut(s)	47, 77, 515, 1136, 1690
TaqII	GACCGA	1 cut(s)	399
TauI	GCSGC	6 cut(s)	142, 170, 191, 284, 335, 1591
TfiI	GAWTC	8 cut(s)	202, 257, 406, 587, 971, 1030, 1167, 1692
Tru1I	TTAA	3 cut(s)	747, 906, 1317
Tru9I	TTAA	3 cut(s)	747, 906, 1317
TscAI	CASTG	5 cut(s)	918, 1065, 1198, 1446, 1458
TseFI	GTSAC	3 cut(s)	130, 1060, 1189
TseI	GCWGC	7 cut(s)	437, 539, 641, 794, 1229, 1505, 1544
Tsp45I	GTSAC	3 cut(s)	130, 1060, 1189
TspDTI	ATGAA	5 cut(s)	1038, 1059, 1073, 1300, 1450
TspGWI	ACGGA	1 cut(s)	30
TspRI	CASTG	5 cut(s)	918, 1065, 1198, 1446, 1458
Van91I	CCANNNNNTGG	1 cut(s)	1653
VneI	GTGCAC	2 cut(s)	1283, 1444
VpaK11BI	GGWCC	4 cut(s)	19, 97, 411, 922
XapI	RAATTY	1 cut(s)	818
XceI	RCATGY	2 cut(s)	659, 842
XmnI	GAANNNNTTC	1 cut(s)	954
Zsp2I	ATGCAT	1 cut(s)	631

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.