pycom01g14570

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010764
Physical Location & Seq
Reverse (-)
14696743 .. 14699992
3250 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g14570.1

Sequence Viewer

Length: 1938 bp
ATGAACAATCCAATAAAATATCATCATCAATCAACAACTTGTCTTTTGTTTATTACTTTTGTCACATGCTTAATTTCATCATTCCTGATTGAACCCATATTTTATTTTCCCGGAAAGTTGCCTCCTTTATTTTCCTCCGAGCCAAACAGATTTTTTTTTATTTTTCTGAATATTTGCGTATTTTTTGTGATTATGATCGAACTTGTAGGAGGTGATGGGGATTTAGAGAAGGGTCCGATGACTGCACAGCCAAACCAGAACTCTCTGGCGGAGCCATCGCCGTCGCCGTCTTCCACGACGTTGGCGCCGGCTTTGGTGCTTTCCAACTCCGGAAAGCGGATGGACCAGGCGGGGAAGAAGAAGTACTTGAAGCAGGTCACCGGGCGGCACAACGACACGGAGCTCCACTTGGCGGCTCAGCGCGCGGATCTAGCCGCCGTGAAGAAGATTCTCGGCGATATCGATTCGCAGATTGTGGGGACAGTTAGCGGTGCGGAGTTTGATACGGAGGTGGCTGAGGTTCGGGCGGCGGTGGTGAATGAGGTCAATGAGCTGGGAGAGACGGCTTTGTTCACGGCGGCGGATAAGGGGCACCTTGATGTGGTTAAGGAGCTGTTGAAATATTCGAATCGGGAGACCGTTACGAAGAAGAACCGGTCCGGGTTTGATCCCTTGCATGCTGCTGCAAGTCAAGGACACCACGCCATTGTCCAGGTGCTCCTAGATCATGACCCTGGGCTAAGCAAAACAATTGGTCCATCGAATTCAACCCCACTTATATCTGCAGCTCATAAAGGGCATATAGCGGTAGTTGATGAACTGCTCTCGAAAGATTCTACCTTGTTGGAGATTTCTAAATCAAATGGGAAGAATGCATTGCATCTGGCTGCAAGACAGGGGCATACAGAGATTGTAAGCGCATTGCTAACTAAGGATCCACAGTTGGCACGAAGAACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAAGGGACGAACTGCGAGGTGGTGAAATTGCTTCTTGAGGCAGACCCCGCAATTGTAATGCTGCCAGACAAATTTGGTAACACAGCATTACATATTGCCACCAGGAAGAAGCGAGCAGAGATAGTGAATGAGTTGTTACTCCTCCCAGACACCAATGTTAATGCACTGAACAGGGATACAAAAACGGCTCTTGACATTGCTGATGCACTTCCACTTTCCGAAGAATCCTCAGAAATAAGGGGTTGTCTTTATCGCTATGGTGCTGTCAAGGCAAATGAACTGAACCAACCAAGGGATGAATTGAGGAAAACGGTCACTCAGATCAAGAACGATGTTCATATCCAGCTTGAACAAACAAGGAAAACCAACAAAAATGTTCACAATATATCCAAAGAGCTGAGAAAGCTCCACAGGGAAGGAATCAACAACGCCACCAACTCAGTCACTGTGGTGGCTGTTCTATTCGCAACAGTTGCCTTTGCAGCTATCTTTACGGTGCCTGGCGGTGATAATAATGACGGGACTGCCGTGGTAGTTAAGAGCACTCCTTTCAAAATCTTCTTCATTTTCAATGCCATTGCCCTTTTTACGTCGCTGGCTGTTGTGGTTGTCCAAATTACCTTGGTCAGAGGCGAGACAAAGGCAGAGAAACGGGTGGTGGAGATAATCAACAAGTTGATGTGGCTGGCTTCAGTCTGCACTTCAGTGGCATTCATGGCCTCCTCTTACATAGTGGTTGGCCAGCGCCATAAGTGGGCCGCCATTCTGGTAACAGTCGTTGGGGGAATGATAATGGCTGCTGTTCTCGGCACCATGACATACTACGTGGTGAAGTCGAAGCGGATACGTTCCATGAGGAAGAAGCACCCGAAGAGGAGCGGTTCCAACTCGTGGATGCACTCTGACCATTCAAATTCGGAAATCGAACGGATTTATGCCCTTTGA

Protein Analysis

646

Amino Acids

70.63

Weight (kDa)

9.23

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 135 - 244 2.6e-14 Ankyrin repeats (3 copies)
Ank_KRIT1 PF24521 186 - 278 3.7e-07 KRIT1 ankyrin-repeats domain
Ank_4 PF13637 190 - 241 1.3e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 250 - 312 9.6e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 259 - 346 6.4e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 260 - 309 8.8e-06 Ankyrin repeats (many copies)
Ank_KRIT1 PF24521 281 - 379 7.9e-06 KRIT1 ankyrin-repeats domain
Ank_2 PF12796 318 - 385 1.8e-11 Ankyrin repeats (3 copies)
Ank_5 PF13857 342 - 398 8.8e-08 Ankyrin repeats (many copies)
PGG PF13962 470 - 576 5.3e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 364
AccB1I GGYRCC 4 cut(s) 304, 591, 1489, 1802
AccB7I CCANNNNNTGG 1 cut(s) 1884
AccBSI CCGCTC 1 cut(s) 1872
AccII CGCG 2 cut(s) 423, 425
AccIII TCCGGA 1 cut(s) 329
AclWI GGATC 4 cut(s) 435, 662, 929, 942
AcoI YGGCCR 1 cut(s) 1732
AcsI RAATTY 3 cut(s) 763, 1064, 1906
AcuI CTGAAG 2 cut(s) 1668, 1680
AcyI GRCGYC 1 cut(s) 305
AfaI GTAC 1 cut(s) 365
AfiI CCNNNNNNNGG 6 cut(s) 336, 412, 601, 1285, 1747, 1884
AgeI ACCGGT 1 cut(s) 654
AgsI TTSAA 8 cut(s) 92, 370, 619, 768, 1343, 1546, 1564, 1905
AjnI CCWGG 5 cut(s) 345, 711, 733, 1094, 1492
AleI CACNNNNGTG 2 cut(s) 1442, 1697
AluBI AGCT 8 cut(s) 403, 553, 613, 788, 1339, 1390, 1399, 1478
AluI AGCT 8 cut(s) 403, 553, 613, 788, 1339, 1390, 1399, 1478
Alw21I GWGCWC 3 cut(s) 405, 720, 1538
Alw26I GTCTC 3 cut(s) 554, 629, 1622
AlwI GGATC 4 cut(s) 435, 662, 929, 942
AlwNI CAGNNNCTG 1 cut(s) 1439
Aor13HI TCCGGA 1 cut(s) 329
AoxI GGCC 3 cut(s) 1710, 1732, 1749
ApeKI GCWGC 7 cut(s) 680, 683, 785, 887, 1054, 1475, 1790
ApoI RAATTY 3 cut(s) 763, 1064, 1906
AsiGI ACCGGT 1 cut(s) 654
AspLEI GCGC 5 cut(s) 307, 423, 425, 920, 1740
AspS9I GGNCC 5 cut(s) 233, 343, 657, 755, 1749
AsuC2I CCSGG 3 cut(s) 111, 382, 661
AsuHPI GGTGA 6 cut(s) 224, 370, 547, 1027, 1511, 1834
AsuII TTCGAA 1 cut(s) 626
AvaII GGWCC 4 cut(s) 233, 343, 657, 755
BaeGI GKGCMC 1 cut(s) 594
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BalI TGGCCA 1 cut(s) 1734
BamHI GGATCC 1 cut(s) 934
BanI GGYRCC 4 cut(s) 304, 591, 1489, 1802
BanII GRGCYC 1 cut(s) 405
BarI GAAGNNNNNNTAC 2 cut(s) 347, 379
BauI CACGAG 1 cut(s) 1882
BbsI GAAGAC 1 cut(s) 282
Bbv12I GWGCWC 3 cut(s) 405, 720, 1538
BbvCI CCTCAGC 1 cut(s) 516
BbvI GCAGC 7 cut(s) 667, 670, 797, 874, 1041, 1487, 1777
BccI CCATC 4 cut(s) 209, 283, 334, 766
BceAI ACGGC 7 cut(s) 265, 271, 422, 579, 591, 1194, 1505
BciT130I CCWGG 5 cut(s) 347, 713, 735, 1096, 1494
BciVI GTATCC 2 cut(s) 1162, 1830
BcnI CCSGG 3 cut(s) 111, 382, 661
BcoDI GTCTC 3 cut(s) 554, 629, 1622
BfaI CTAG 2 cut(s) 431, 722
BfmI CTRYAG 1 cut(s) 783
BfoI RGCGCY 2 cut(s) 308, 1741
BfuAI ACCTGC 1 cut(s) 364
BfuI GTATCC 2 cut(s) 1162, 1830
BlpI GCTNAGC 2 cut(s) 417, 740
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 8 cut(s) 111, 347, 382, 661, 713, 735, 1096, 1494
Bme18I GGWCC 4 cut(s) 233, 343, 657, 755
BmgT120I GGNCC 5 cut(s) 233, 343, 657, 755, 1749
BmiI GGNNCC 8 cut(s) 234, 273, 306, 593, 936, 1491, 1804, 1876
BmrFI CCNGG 8 cut(s) 111, 347, 382, 661, 713, 735, 1096, 1494
BmsI GCATC 3 cut(s) 889, 1186, 1878
BpiI GAAGAC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 516
Bpu1102I GCTNAGC 2 cut(s) 417, 740
Bpu14I TTCGAA 1 cut(s) 626
BpuEI CTTGAG 1 cut(s) 1049
BpuMI CCSGG 3 cut(s) 111, 382, 661
Bsa29I ATCGAT 1 cut(s) 462
BsaAI YACGTR 1 cut(s) 1819
BsaBI GATNNNNATC 1 cut(s) 194
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 629
BsaJI CCNNGG 5 cut(s) 733, 734, 1283, 1521, 1614
BsaWI WCCGGW 2 cut(s) 329, 654
Bsc4I CCNNNNNNNGG 6 cut(s) 336, 412, 601, 1285, 1747, 1884
Bse118I RCCGGY 2 cut(s) 307, 654
Bse3DI GCAATG 5 cut(s) 875, 920, 977, 1188, 1569
Bse8I GATNNNNATC 1 cut(s) 194
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 5 cut(s) 347, 713, 735, 1096, 1494
BseCI ATCGAT 1 cut(s) 462
BseDI CCNNGG 5 cut(s) 733, 734, 1283, 1521, 1614
BseGI GGATG 3 cut(s) 345, 1294, 1893
BseJI GATNNNNATC 1 cut(s) 194
BseLI CCNNNNNNNGG 6 cut(s) 336, 412, 601, 1285, 1747, 1884
BseMI GCAATG 5 cut(s) 875, 920, 977, 1188, 1569
BseMII CTCAG 6 cut(s) 431, 507, 1236, 1325, 1382, 1446
BsePI GCGCGC 1 cut(s) 421
BseRI GAGGAG 3 cut(s) 1124, 1705, 1882
BseSI GKGCMC 1 cut(s) 594
BseXI GCAGC 7 cut(s) 667, 670, 797, 874, 1041, 1487, 1777
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 2 cut(s) 228, 1675
Bsh1236I CGCG 2 cut(s) 423, 425
BshFI GGCC 3 cut(s) 1712, 1734, 1751
BshNI GGYRCC 4 cut(s) 304, 591, 1489, 1802
BshTI ACCGGT 1 cut(s) 654
BshVI ATCGAT 1 cut(s) 462
BsiHKAI GWGCWC 3 cut(s) 405, 720, 1538
BsiSI CCGG 6 cut(s) 111, 308, 330, 381, 655, 660
BslFI GGGAC 3 cut(s) 493, 1012, 1528
BslI CCNNNNNNNGG 6 cut(s) 336, 412, 601, 1285, 1747, 1884
BsmAI GTCTC 3 cut(s) 554, 629, 1622
BsmBI CGTCTC 1 cut(s) 554
BsmFI GGGAC 3 cut(s) 493, 1012, 1528
BsmI GAATGC 2 cut(s) 877, 1703
BsnI GGCC 3 cut(s) 1712, 1734, 1751
Bso31I GGTCTC 1 cut(s) 629
Bsp119I TTCGAA 1 cut(s) 626
Bsp1286I GDGCHC 4 cut(s) 405, 594, 720, 1538
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
Bsp1720I GCTNAGC 2 cut(s) 417, 740
BspANI GGCC 3 cut(s) 1712, 1734, 1751
BspCNI CTCAG 6 cut(s) 430, 508, 1235, 1324, 1383, 1445
BspDI ATCGAT 1 cut(s) 462
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 2 cut(s) 423, 425
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 8 cut(s) 234, 273, 306, 593, 936, 1491, 1804, 1876
BspMAI CTGCAG 1 cut(s) 787
BspMI ACCTGC 1 cut(s) 364
BspPI GGATC 4 cut(s) 435, 662, 929, 942
BspT104I TTCGAA 1 cut(s) 626
BspT107I GGYRCC 4 cut(s) 304, 591, 1489, 1802
BspTNI GGTCTC 1 cut(s) 629
BsrBI CCGCTC 1 cut(s) 1872
BsrDI GCAATG 5 cut(s) 875, 920, 977, 1188, 1569
BsrFI RCCGGY 2 cut(s) 307, 654
BssAI RCCGGY 2 cut(s) 307, 654
BssECI CCNNGG 5 cut(s) 733, 734, 1283, 1521, 1614
BssHII GCGCGC 1 cut(s) 421
BssMI GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
BssNI GRCGYC 1 cut(s) 305
BssSI CACGAG 1 cut(s) 1882
BssT1I CCWWGG 2 cut(s) 1283, 1614
Bst2BI CACGAG 1 cut(s) 1882
Bst2UI CCWGG 5 cut(s) 347, 713, 735, 1096, 1494
Bst4CI ACNGT 8 cut(s) 484, 640, 942, 1306, 1441, 1465, 1489, 1768
Bst6I CTCTTC 1 cut(s) 1859
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 1466
BstBAI YACGTR 1 cut(s) 1819
BstBI TTCGAA 1 cut(s) 626
BstC8I GCNNGC 7 cut(s) 309, 423, 678, 1107, 1590, 1680, 1736
BstDEI CTNAG 8 cut(s) 417, 516, 740, 930, 1222, 1311, 1391, 1432
BstDSI CCRYGG 1 cut(s) 1521
BstEII GGTNACC 1 cut(s) 376
BstF5I GGATG 3 cut(s) 345, 1294, 1893
BstFNI CGCG 2 cut(s) 423, 425
BstH2I RGCGCY 2 cut(s) 308, 1741
BstHHI GCGC 5 cut(s) 307, 423, 425, 920, 1740
BstKTI GATC 6 cut(s) 198, 430, 670, 727, 937, 1317
BstMAI GTCTC 3 cut(s) 554, 629, 1622
BstMBI GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
BstMWI GCNNNNNNNGC 8 cut(s) 422, 431, 1040, 1262, 1396, 1466, 1475, 1709
BstNI CCWGG 5 cut(s) 347, 713, 735, 1096, 1494
BstNSI RCATGY 2 cut(s) 69, 680
BstPI GGTNACC 1 cut(s) 376
BstSCI CCNGG 8 cut(s) 109, 345, 380, 659, 711, 733, 1094, 1492
BstSFI CTRYAG 1 cut(s) 783
BstSLI GKGCMC 1 cut(s) 594
BstUI CGCG 2 cut(s) 423, 425
BstV1I GCAGC 7 cut(s) 667, 670, 797, 874, 1041, 1487, 1777
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 2 cut(s) 427, 934
BstXI CCANNNNNNTGG 1 cut(s) 301
BstYI RGATCY 2 cut(s) 427, 934
Bsu15I ATCGAT 1 cut(s) 462
BsuI GTATCC 2 cut(s) 1162, 1830
BsuRI GGCC 3 cut(s) 1712, 1734, 1751
BsuTUI ATCGAT 1 cut(s) 462
BtgI CCRYGG 1 cut(s) 1521
BtgZI GCGATG 1 cut(s) 261
BtsCI GGATG 3 cut(s) 345, 1294, 1893
BtsIMutI CAGTG 3 cut(s) 1157, 1437, 1704
BveI ACCTGC 1 cut(s) 364
Cac8I GCNNGC 7 cut(s) 309, 423, 678, 1107, 1590, 1680, 1736
CaiI CAGNNNCTG 1 cut(s) 1439
CciI TCATGA 1 cut(s) 727
CfoI GCGC 5 cut(s) 307, 423, 425, 920, 1740
Cfr10I RCCGGY 2 cut(s) 307, 654
Cfr13I GGNCC 5 cut(s) 233, 343, 657, 755, 1749
ClaI ATCGAT 1 cut(s) 462
CpoI CGGWCCG 1 cut(s) 657
Csp6I GTAC 1 cut(s) 364
CspAI ACCGGT 1 cut(s) 654
CspI CGGWCCG 1 cut(s) 657
CviAII CATG 6 cut(s) 66, 677, 728, 1708, 1807, 1846
CviQI GTAC 1 cut(s) 364
DdeI CTNAG 8 cut(s) 417, 516, 740, 930, 1222, 1311, 1391, 1432
DinI GGCGCC 1 cut(s) 306
DpnI GATC 6 cut(s) 197, 429, 669, 726, 936, 1316
DpnII GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
EaeI YGGCCR 1 cut(s) 1732
Eam1104I CTCTTC 1 cut(s) 1859
EarI CTCTTC 1 cut(s) 1859
EciI GGCGGA 2 cut(s) 284, 596
Ecl136II GAGCTC 1 cut(s) 403
Eco130I CCWWGG 2 cut(s) 1283, 1614
Eco24I GRGCYC 1 cut(s) 405
Eco31I GGTCTC 1 cut(s) 629
Eco32I GATATC 1 cut(s) 460
Eco47I GGWCC 4 cut(s) 233, 343, 657, 755
Eco53kI GAGCTC 1 cut(s) 403
Eco57I CTGAAG 2 cut(s) 1668, 1680
Eco91I GGTNACC 1 cut(s) 376
EcoICRI GAGCTC 1 cut(s) 403
EcoO65I GGTNACC 1 cut(s) 376
EcoRI GAATTC 1 cut(s) 763
EcoRII CCWGG 5 cut(s) 345, 711, 733, 1094, 1492
EcoRV GATATC 1 cut(s) 460
EcoT14I CCWWGG 2 cut(s) 1283, 1614
EcoT22I ATGCAT 1 cut(s) 877
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 2 cut(s) 1283, 1614
Esp3I CGTCTC 1 cut(s) 554
FaeI CATG 6 cut(s) 69, 680, 731, 1711, 1810, 1849
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 3 cut(s) 493, 1012, 1528
FatI CATG 6 cut(s) 65, 676, 727, 1707, 1806, 1845
FauI CCCGC 2 cut(s) 343, 1048
FauNDI CATATG 1 cut(s) 984
FokI GGATG 3 cut(s) 352, 1301, 1900
FriOI GRGCYC 1 cut(s) 405
FspBI CTAG 2 cut(s) 431, 722
GlaI GCGC 5 cut(s) 306, 422, 424, 919, 1739
GsaI CCCAGC 1 cut(s) 557
HaeII RGCGCY 2 cut(s) 308, 1741
HaeIII GGCC 3 cut(s) 1712, 1734, 1751
HapII CCGG 6 cut(s) 111, 308, 330, 381, 655, 660
HhaI GCGC 5 cut(s) 307, 423, 425, 920, 1740
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 6 cut(s) 69, 680, 731, 1711, 1810, 1849
Hin6I GCGC 5 cut(s) 305, 421, 423, 918, 1738
HinP1I GCGC 5 cut(s) 305, 421, 423, 918, 1738
HinfI GANTC 6 cut(s) 448, 464, 628, 833, 1217, 1413
HpaII CCGG 6 cut(s) 111, 308, 330, 381, 655, 660
HphI GGTGA 6 cut(s) 224, 370, 547, 1027, 1511, 1834
Hpy166II GTNNAC 2 cut(s) 573, 1372
Hpy188I TCNGA 9 cut(s) 139, 168, 237, 1213, 1225, 1314, 1622, 1897, 1912
Hpy188III TCNNGA 8 cut(s) 85, 330, 632, 728, 826, 1028, 1184, 1318
Hpy8I GTNNAC 2 cut(s) 573, 1372
Hpy99I CGWCG 3 cut(s) 286, 301, 1588
HpyAV CCTTC 2 cut(s) 223, 1403
HpyCH4III ACNGT 8 cut(s) 484, 640, 942, 1306, 1441, 1465, 1489, 1768
HpyCH4IV ACGT 4 cut(s) 299, 1583, 1818, 1840
HpyF10VI GCNNNNNNNGC 8 cut(s) 422, 431, 1040, 1262, 1396, 1466, 1475, 1709
HpyF3I CTNAG 8 cut(s) 417, 516, 740, 930, 1222, 1311, 1391, 1432
HpySE526I ACGT 4 cut(s) 299, 1583, 1818, 1840
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 6 cut(s) 69, 680, 731, 1711, 1810, 1849
HspAI GCGC 5 cut(s) 305, 421, 423, 918, 1738
KasI GGCGCC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 329
KroI GCCGGC 1 cut(s) 307
KroNI GCCGGC 1 cut(s) 309
Kzo9I GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
LmnI GCTCC 7 cut(s) 271, 400, 408, 610, 723, 1404, 1869
Lsp1109I GCAGC 7 cut(s) 667, 670, 797, 874, 1041, 1487, 1777
LweI GCATC 3 cut(s) 889, 1186, 1878
MaeI CTAG 2 cut(s) 431, 722
MaeII ACGT 4 cut(s) 299, 1583, 1818, 1840
MaeIII GTNAC 8 cut(s) 61, 376, 640, 1070, 1128, 1306, 1435, 1762
MalI GATC 6 cut(s) 197, 429, 669, 726, 936, 1316
MbiI CCGCTC 1 cut(s) 1872
MboI GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
MfeI CAATTG 2 cut(s) 750, 1044
MflI RGATCY 2 cut(s) 427, 934
MhlI GDGCHC 4 cut(s) 405, 594, 720, 1538
MlsI TGGCCA 1 cut(s) 1734
MluCI AATT 9 cut(s) 72, 750, 763, 1019, 1044, 1064, 1292, 1608, 1906
MluNI TGGCCA 1 cut(s) 1734
Mly113I GGCGCC 1 cut(s) 305
MmeI TCCRAC 3 cut(s) 348, 825, 1902
Mox20I TGGCCA 1 cut(s) 1734
Mph1103I ATGCAT 1 cut(s) 877
MroI TCCGGA 1 cut(s) 329
MroNI GCCGGC 1 cut(s) 307
MscI TGGCCA 1 cut(s) 1734
MseI TTAA 4 cut(s) 71, 606, 1152, 1530
MslI CAYNNNNRTG 3 cut(s) 597, 1442, 1697
Msp20I TGGCCA 1 cut(s) 1734
MspI CCGG 6 cut(s) 111, 308, 330, 381, 655, 660
MspR9I CCNGG 8 cut(s) 111, 347, 382, 661, 713, 735, 1096, 1494
MunI CAATTG 2 cut(s) 750, 1044
Mva1269I GAATGC 2 cut(s) 877, 1703
MvaI CCWGG 5 cut(s) 347, 713, 735, 1096, 1494
MvnI CGCG 2 cut(s) 423, 425
MwoI GCNNNNNNNGC 8 cut(s) 422, 431, 1040, 1262, 1396, 1466, 1475, 1709
NaeI GCCGGC 1 cut(s) 309
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 3 cut(s) 111, 382, 661
NdeI CATATG 1 cut(s) 984
NdeII GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
NgoMIV GCCGGC 1 cut(s) 307
NlaIII CATG 6 cut(s) 69, 680, 731, 1711, 1810, 1849
NlaIV GGNNCC 8 cut(s) 234, 273, 306, 593, 936, 1491, 1804, 1876
NmeAIII GCCGAG 2 cut(s) 432, 1779
NmuCI GTSAC 4 cut(s) 61, 376, 1306, 1435
NsiI ATGCAT 1 cut(s) 877
NspI RCATGY 2 cut(s) 69, 680
NspV TTCGAA 1 cut(s) 626
OliI CACNNNNGTG 2 cut(s) 1442, 1697
PaeI GCATGC 1 cut(s) 680
PagI TCATGA 1 cut(s) 727
PasI CCCWGGG 1 cut(s) 734
PauI GCGCGC 1 cut(s) 421
PcsI WCGNNNNNNNCGW 3 cut(s) 284, 459, 1518
PctI GAATGC 2 cut(s) 877, 1703
PdiI GCCGGC 1 cut(s) 309
PfeI GAWTC 6 cut(s) 448, 464, 628, 833, 1217, 1413
PflMI CCANNNNNTGG 1 cut(s) 1884
PfoI TCCNGGA 1 cut(s) 109
PinAI ACCGGT 1 cut(s) 654
PluTI GGCGCC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 1819
Psp124BI GAGCTC 1 cut(s) 405
Psp6I CCWGG 5 cut(s) 345, 711, 733, 1094, 1492
PspEI GGTNACC 1 cut(s) 376
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 5 cut(s) 345, 711, 733, 1094, 1492
PspN4I GGNNCC 8 cut(s) 234, 273, 306, 593, 936, 1491, 1804, 1876
PspPI GGNCC 5 cut(s) 233, 343, 657, 755, 1749
PstI CTGCAG 1 cut(s) 787
PstNI CAGNNNCTG 1 cut(s) 1439
PsuI RGATCY 2 cut(s) 427, 934
PteI GCGCGC 1 cut(s) 421
RsaI GTAC 1 cut(s) 365
RsaNI GTAC 1 cut(s) 364
RseI CAYNNNNRTG 3 cut(s) 597, 1442, 1697
Rsr2I CGGWCCG 1 cut(s) 657
RsrII CGGWCCG 1 cut(s) 657
SacI GAGCTC 1 cut(s) 405
SaqAI TTAA 4 cut(s) 71, 606, 1152, 1530
Sau3AI GATC 6 cut(s) 195, 427, 667, 724, 934, 1314
Sau96I GGNCC 5 cut(s) 233, 343, 657, 755, 1749
ScaI AGTACT 1 cut(s) 365
ScrFI CCNGG 8 cut(s) 111, 347, 382, 661, 713, 735, 1096, 1494
SduI GDGCHC 4 cut(s) 405, 594, 720, 1538
SfaNI GCATC 3 cut(s) 889, 1186, 1878
SfcI CTRYAG 1 cut(s) 783
SfoI GGCGCC 1 cut(s) 306
SfuI TTCGAA 1 cut(s) 626
SinI GGWCC 4 cut(s) 233, 343, 657, 755
SmiMI CAYNNNNRTG 3 cut(s) 597, 1442, 1697
SmlI CTYRAG 1 cut(s) 1028
SmoI CTYRAG 1 cut(s) 1028
SphI GCATGC 1 cut(s) 680
Sse9I AATT 9 cut(s) 72, 750, 763, 1019, 1044, 1064, 1292, 1608, 1906
SspDI GGCGCC 1 cut(s) 304
SspI AATATT 2 cut(s) 172, 623
SspMI CTAG 2 cut(s) 431, 722
SstI GAGCTC 1 cut(s) 405
StyD4I CCNGG 8 cut(s) 109, 345, 380, 659, 711, 733, 1094, 1492
StyI CCWWGG 2 cut(s) 1283, 1614
TaaI ACNGT 8 cut(s) 484, 640, 942, 1306, 1441, 1465, 1489, 1768
TaiI ACGT 4 cut(s) 302, 1586, 1821, 1843
TaqI TCGA 7 cut(s) 198, 462, 626, 761, 827, 1829, 1917
TasI AATT 9 cut(s) 72, 750, 763, 1019, 1044, 1064, 1292, 1608, 1906
TatI WGTACW 1 cut(s) 363
TauI GCSGC 6 cut(s) 388, 416, 437, 530, 581, 1754
TfiI GAWTC 6 cut(s) 448, 464, 628, 833, 1217, 1413
Tru1I TTAA 4 cut(s) 71, 606, 1152, 1530
Tru9I TTAA 4 cut(s) 71, 606, 1152, 1530
TscAI CASTG 3 cut(s) 1164, 1444, 1704
TseFI GTSAC 4 cut(s) 61, 376, 1306, 1435
TseI GCWGC 7 cut(s) 680, 683, 785, 887, 1054, 1475, 1790
Tsp45I GTSAC 4 cut(s) 61, 376, 1306, 1435
TspDTI ATGAA 8 cut(s) 17, 66, 831, 1284, 1305, 1319, 1546, 1696
TspGWI ACGGA 3 cut(s) 413, 521, 1936
TspRI CASTG 3 cut(s) 1164, 1444, 1704
Van91I CCANNNNNTGG 1 cut(s) 1884
VpaK11BI GGWCC 4 cut(s) 233, 343, 657, 755
XapI RAATTY 3 cut(s) 763, 1064, 1906
XceI RCATGY 2 cut(s) 69, 680
XspI CTAG 2 cut(s) 431, 722
ZrmI AGTACT 1 cut(s) 365
Zsp2I ATGCAT 1 cut(s) 877
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.