MD07G1186700.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0010764
Physical Location & Seq
Reverse (-)
26772583 .. 26777633
5051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1186700.v1.1.491

Sequence Viewer

Length: 1815 bp
ATGGCGTCCCCAATTGAGCAAGGAGGTGATACGGATTTAGGGAAGGGTCTGATGACTGCACAGCCGAGCCAAAACCCTCTGGCCGAGCCATCGCCGTCGCCGTCTCCGTCTTCCACGGCGTCAGCGCCGGCTTTAGTGCTTTCCAACTCGCCGTCGCCGTCTTCCACCGCGTCGGCGCCGGCTTTGGTGCTTTCCAACTCAGGAAAGCGGATTGACCAGGCGGGGAAGAAGAAGTACGTGAAGCAGGTGACGGGGCGGCACAACGACACGGAGCTTCACTTGGCGGCTCAGCGCGCGGATCTAGCCGCCGTAAAGAAGATTCTCGGCGATATCGATTCGCAGATGGTGGGGACCGTAAGCGGTGCGGAGTTTGATACGGAGGTGGCTGAGGTCCGGGCGGCGGTGGTGAATGAGGTGAATGAGCTGGGAGAGACGGCTTTGTTCACGGCGGCGGATAAAGGGCACCTTGATGTGGTTAAGGAGCTGTTGAAGTATTCGAACAAGGAGACCGTTACGAAGAAGAACAGGTCCGGGTTCGACCCCTTGCATGCTGCTGCAAGTCAAGGACACCATGCCATTGTCCAGGTACTCCTAGATCATGATCCTGGGCTAAGCAAAACACTGGGCCCGGCAAATTCAACCCCACTTATATCCGCAGCTCATAAAGGGCATGTAGCGGTAGTTGATGAACTGCTGTCGAAGGATTCTACCTTGTTGGAAATTTCTAGATCAAACGGGAAGAATGCATTGCATCTAGCTGCAAGACAGGGGCATGCGGAGATTGTAAGGGCATTGCTTACAAAGGATCCACAATTGGCCCGAAGAACTGACAAGAAAGGGCAAACTGCATTGCATATGGCTGTAAAGGGGACAAACTGTGAGGTGGTGAAATTGCTACTAGGGGCAGACCCCGCAATTGTAATGCTTCCAGACAAATTTGGGAACACAGCGTTGCATATTGCCACCAGGAAGAAGCGAGCAGAGATAGTGAATGAGTTGTTACTCCTCCCAGACAGCAATGTTAATGCACTGAACAGAGACACAAAAACGGCTCTTGACATTGCTGATGCACTTCCACTTTCCGAAGAAGCCTCGGAAATAAGGGGTTGCCTTTATCGCTATGGTGCTGTGAAGGCTAATGAACTGAACCAACCAAGGGATGAACTGAGAAAGACTGTGACTCAGATCAAGAACGATGTCCATATCCAGCTTGAACAAACAAAGAAAACCAATAAAAACGTTCACAATATTTCCAAAGAACTTAGAAAGCTCCACAGGGAAGGAATCAACAACGCCACTAACTCAGTGACTGTGGTCGCTGTTCTATTCGCAACAGTTGCCTTTGCCGCTATCTTTACTGTGCCAGGTGGTGATCATGATGACGGGACTGCCGTGGTAGTGAAGGGCCTTCCTTTCAAAATCTTCTTCATTTTCAACGCCATTGCCCTCTTTACGTCTTTGGCTGTTGTGGTTGTCCAAATTACCTTGGTCAGAGGCGAGACAAAGGCAGAGAAACGGGTGGTGGAGATAATAAACAAGTTGATGTGGCTGGCTTCAGTCTGCACTTCGGTGGCATTCATAGCCTCCTCTTACATAGTGGTTGGCCAGCGCCACAAATGGGCCGCCATTCTTGTAACAATGGTTGGGGGAATGATAATGGCGGCTGTTCTCGGCACCATGACATACTACGTGGTGAAGTCAAAGCGGACACGGTCCATGAGGAAGAGGGAGAAGCACACGAGGAGGAGCGGTTCCAACTCATGGATGCCCTCTGACTATTCCAATTCGGAAATCGACCGAATTTATGCCCTTTGA

Protein Analysis

605

Amino Acids

65.09

Weight (kDa)

9.4

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 92 - 201 2.2e-14 Ankyrin repeats (3 copies)
Ank_KRIT1 PF24521 143 - 235 3.7e-07 KRIT1 ankyrin-repeats domain
Ank_4 PF13637 147 - 198 8.7e-08 Ankyrin repeats (many copies)
Ank_2 PF12796 211 - 269 5.3e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 216 - 304 6.8e-17 Ankyrin repeats (3 copies)
Ank_4 PF13637 217 - 266 7e-06 Ankyrin repeats (many copies)
Ank_5 PF13857 304 - 355 1.7e-07 Ankyrin repeats (many copies)
PGG PF13962 427 - 533 1.1e-28 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 235
AccB1I GGYRCC 3 cut(s) 175, 462, 1673
AccB7I CCANNNNNTGG 1 cut(s) 1761
AccBSI CCGCTC 1 cut(s) 1749
AccII CGCG 3 cut(s) 170, 294, 296
AclI AACGTT 1 cut(s) 1239
AclWI GGATC 4 cut(s) 306, 596, 800, 813
AcoI YGGCCR 2 cut(s) 81, 1603
AcsI RAATTY 4 cut(s) 634, 720, 935, 1800
AcuI CTGAAG 1 cut(s) 1539
AcyI GRCGYC 3 cut(s) 5, 119, 176
AfaI GTAC 2 cut(s) 236, 588
AfiI CCNNNNNNNGG 5 cut(s) 400, 472, 1156, 1618, 1761
AgsI TTSAA 5 cut(s) 490, 639, 1214, 1417, 1435
AjnI CCWGG 5 cut(s) 216, 582, 604, 965, 1363
AleI CACNNNNGTG 1 cut(s) 1568
AloI GAACNNNNNNTCC 2 cut(s) 1735, 1767
AluBI AGCT 7 cut(s) 274, 424, 484, 659, 758, 1210, 1270
AluI AGCT 7 cut(s) 274, 424, 484, 659, 758, 1210, 1270
Alw26I GTCTC 5 cut(s) 108, 425, 500, 1032, 1493
AlwI GGATC 4 cut(s) 306, 596, 800, 813
AlwNI CAGNNNCTG 1 cut(s) 1310
AoxI GGCC 6 cut(s) 81, 625, 816, 1405, 1603, 1620
ApaI GGGCCC 1 cut(s) 629
ApeKI GCWGC 4 cut(s) 551, 554, 656, 758
ApoI RAATTY 4 cut(s) 634, 720, 935, 1800
AspLEI GCGC 5 cut(s) 127, 178, 294, 296, 1611
AspS9I GGNCC 9 cut(s) 351, 391, 528, 625, 626, 817, 1405, 1620, 1713
AsuC2I CCSGG 3 cut(s) 395, 532, 629
AsuHPI GGTGA 7 cut(s) 38, 259, 418, 427, 898, 1382, 1705
AsuII TTCGAA 1 cut(s) 497
AvaII GGWCC 4 cut(s) 351, 391, 528, 1713
BaeGI GKGCMC 2 cut(s) 465, 629
BaeI ACNNNNGTAYC 2 cut(s) 366, 399
BalI TGGCCA 1 cut(s) 1605
BamHI GGATCC 1 cut(s) 805
BanI GGYRCC 3 cut(s) 175, 462, 1673
BanII GRGCYC 1 cut(s) 629
BarI GAAGNNNNNNTAC 2 cut(s) 218, 250
BauI CACGAG 1 cut(s) 1738
BbsI GAAGAC 2 cut(s) 102, 153
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 4 cut(s) 538, 541, 668, 745
BccI CCATC 2 cut(s) 97, 337
BciT130I CCWGG 5 cut(s) 218, 584, 606, 967, 1365
BclI TGATCA 1 cut(s) 1372
BcnI CCSGG 3 cut(s) 395, 532, 629
BcoDI GTCTC 5 cut(s) 108, 425, 500, 1032, 1493
BfaI CTAG 5 cut(s) 302, 593, 726, 755, 899
BfoI RGCGCY 3 cut(s) 128, 179, 1612
BfuAI ACCTGC 1 cut(s) 235
BlpI GCTNAGC 2 cut(s) 288, 611
Bme1390I CCNGG 8 cut(s) 218, 395, 532, 584, 606, 629, 967, 1365
Bme18I GGWCC 4 cut(s) 351, 391, 528, 1713
BmgT120I GGNCC 9 cut(s) 351, 391, 528, 625, 626, 817, 1405, 1620, 1713
BmiI GGNNCC 7 cut(s) 177, 352, 464, 627, 807, 1675, 1753
BmrFI CCNGG 8 cut(s) 218, 395, 532, 584, 606, 629, 967, 1365
BmrI ACTGGG 1 cut(s) 632
BmsI GCATC 3 cut(s) 760, 1057, 1755
BmuI ACTGGG 1 cut(s) 632
BoxI GACNNNNGTC 1 cut(s) 1313
BpiI GAAGAC 2 cut(s) 102, 153
Bpu10I CCTNAGC 1 cut(s) 387
Bpu1102I GCTNAGC 2 cut(s) 288, 611
Bpu14I TTCGAA 1 cut(s) 497
BpuMI CCSGG 3 cut(s) 395, 532, 629
Bsa29I ATCGAT 1 cut(s) 333
BsaAI YACGTR 2 cut(s) 238, 1690
BsaBI GATNNNNATC 1 cut(s) 600
BsaHI GRCGYC 3 cut(s) 5, 119, 176
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 6 cut(s) 114, 605, 1092, 1154, 1392, 1485
BsaXI ACNNNNNCTCC 2 cut(s) 1735, 1765
Bsc4I CCNNNNNNNGG 5 cut(s) 400, 472, 1156, 1618, 1761
Bse118I RCCGGY 2 cut(s) 127, 178
Bse1I ACTGG 1 cut(s) 627
Bse3DI GCAATG 6 cut(s) 746, 791, 848, 1024, 1059, 1440
Bse8I GATNNNNATC 1 cut(s) 600
BseBI CCWGG 5 cut(s) 218, 584, 606, 967, 1365
BseCI ATCGAT 1 cut(s) 333
BseDI CCNNGG 6 cut(s) 114, 605, 1092, 1154, 1392, 1485
BseGI GGATG 2 cut(s) 1165, 1770
BseJI GATNNNNATC 1 cut(s) 600
BseLI CCNNNNNNNGG 5 cut(s) 400, 472, 1156, 1618, 1761
BseMI GCAATG 6 cut(s) 746, 791, 848, 1024, 1059, 1440
BseMII CTCAG 6 cut(s) 213, 302, 378, 1157, 1196, 1317
BseNI ACTGG 1 cut(s) 627
BsePI GCGCGC 1 cut(s) 292
BseRI GAGGAG 4 cut(s) 995, 1576, 1756, 1759
BseSI GKGCMC 2 cut(s) 465, 629
BseXI GCAGC 4 cut(s) 538, 541, 668, 745
BseYI CCCAGC 1 cut(s) 424
BsgI GTGCAG 2 cut(s) 42, 1546
Bsh1236I CGCG 3 cut(s) 170, 294, 296
Bsh1285I CGRYCG 1 cut(s) 1798
BshFI GGCC 6 cut(s) 83, 627, 818, 1407, 1605, 1622
BshNI GGYRCC 3 cut(s) 175, 462, 1673
BshVI ATCGAT 1 cut(s) 333
BsiEI CGRYCG 1 cut(s) 1798
BsiSI CCGG 5 cut(s) 128, 179, 394, 531, 629
BslFI GGGAC 3 cut(s) 364, 883, 1399
BslI CCNNNNNNNGG 5 cut(s) 400, 472, 1156, 1618, 1761
BsmAI GTCTC 5 cut(s) 108, 425, 500, 1032, 1493
BsmBI CGTCTC 2 cut(s) 108, 425
BsmFI GGGAC 3 cut(s) 364, 883, 1399
BsmI GAATGC 2 cut(s) 748, 1574
BsnI GGCC 6 cut(s) 83, 627, 818, 1407, 1605, 1622
Bso31I GGTCTC 1 cut(s) 500
Bsp119I TTCGAA 1 cut(s) 497
Bsp120I GGGCCC 1 cut(s) 625
Bsp1286I GDGCHC 2 cut(s) 465, 629
Bsp143I GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
Bsp1720I GCTNAGC 2 cut(s) 288, 611
BspANI GGCC 6 cut(s) 83, 627, 818, 1407, 1605, 1622
BspCNI CTCAG 6 cut(s) 212, 301, 379, 1158, 1195, 1316
BspDI ATCGAT 1 cut(s) 333
BspFNI CGCG 3 cut(s) 170, 294, 296
BspHI TCATGA 2 cut(s) 598, 1375
BspLI GGNNCC 7 cut(s) 177, 352, 464, 627, 807, 1675, 1753
BspMI ACCTGC 1 cut(s) 235
BspPI GGATC 4 cut(s) 306, 596, 800, 813
BspT104I TTCGAA 1 cut(s) 497
BspT107I GGYRCC 3 cut(s) 175, 462, 1673
BspTNI GGTCTC 1 cut(s) 500
BsrBI CCGCTC 1 cut(s) 1749
BsrDI GCAATG 6 cut(s) 746, 791, 848, 1024, 1059, 1440
BsrFI RCCGGY 2 cut(s) 127, 178
BsrI ACTGG 1 cut(s) 627
BssAI RCCGGY 2 cut(s) 127, 178
BssECI CCNNGG 6 cut(s) 114, 605, 1092, 1154, 1392, 1485
BssHII GCGCGC 1 cut(s) 292
BssMI GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
BssNI GRCGYC 3 cut(s) 5, 119, 176
BssSI CACGAG 1 cut(s) 1738
BssT1I CCWWGG 2 cut(s) 1154, 1485
Bst2BI CACGAG 1 cut(s) 1738
Bst2UI CCWGG 5 cut(s) 218, 584, 606, 967, 1365
Bst4CI ACNGT 8 cut(s) 355, 511, 878, 1177, 1312, 1336, 1360, 1713
Bst6I CTCTTC 1 cut(s) 1718
BstACI GRCGYC 3 cut(s) 5, 119, 176
BstAPI GCANNNNNTGC 1 cut(s) 1337
BstBAI YACGTR 2 cut(s) 238, 1690
BstBI TTCGAA 1 cut(s) 497
BstC8I GCNNGC 8 cut(s) 129, 180, 294, 549, 774, 978, 1551, 1607
BstDEI CTNAG 8 cut(s) 199, 288, 387, 611, 1166, 1182, 1262, 1303
BstDSI CCRYGG 2 cut(s) 114, 1392
BstF5I GGATG 2 cut(s) 1165, 1770
BstFNI CGCG 3 cut(s) 170, 294, 296
BstH2I RGCGCY 3 cut(s) 128, 179, 1612
BstHHI GCGC 5 cut(s) 127, 178, 294, 296, 1611
BstKTI GATC 7 cut(s) 301, 598, 604, 731, 808, 1188, 1375
BstMAI GTCTC 5 cut(s) 108, 425, 500, 1032, 1493
BstMBI GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
BstMCI CGRYCG 1 cut(s) 1798
BstMWI GCNNNNNNNGC 8 cut(s) 293, 302, 911, 1116, 1133, 1337, 1346, 1580
BstNI CCWGG 5 cut(s) 218, 584, 606, 967, 1365
BstNSI RCATGY 3 cut(s) 551, 674, 776
BstPAI GACNNNNGTC 1 cut(s) 1313
BstSCI CCNGG 8 cut(s) 216, 393, 530, 582, 604, 627, 965, 1363
BstSLI GKGCMC 2 cut(s) 465, 629
BstUI CGCG 3 cut(s) 170, 294, 296
BstV1I GCAGC 4 cut(s) 538, 541, 668, 745
BstV2I GAAGAC 2 cut(s) 102, 153
BstX2I RGATCY 2 cut(s) 298, 805
BstYI RGATCY 2 cut(s) 298, 805
Bsu15I ATCGAT 1 cut(s) 333
BsuRI GGCC 6 cut(s) 83, 627, 818, 1407, 1605, 1622
BsuTUI ATCGAT 1 cut(s) 333
BtgI CCRYGG 2 cut(s) 114, 1392
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 2 cut(s) 1165, 1770
BtsIMutI CAGTG 3 cut(s) 620, 1028, 1311
BveI ACCTGC 1 cut(s) 235
Cac8I GCNNGC 8 cut(s) 129, 180, 294, 549, 774, 978, 1551, 1607
CaiI CAGNNNCTG 1 cut(s) 1310
CciI TCATGA 2 cut(s) 598, 1375
CfoI GCGC 5 cut(s) 127, 178, 294, 296, 1611
Cfr10I RCCGGY 2 cut(s) 127, 178
Cfr13I GGNCC 9 cut(s) 351, 391, 528, 625, 626, 817, 1405, 1620, 1713
ClaI ATCGAT 1 cut(s) 333
CseI GACGC 2 cut(s) 108, 159
Csp6I GTAC 2 cut(s) 235, 587
CviAII CATG 9 cut(s) 548, 572, 599, 671, 773, 1376, 1678, 1717, 1761
CviQI GTAC 2 cut(s) 235, 587
DdeI CTNAG 8 cut(s) 199, 288, 387, 611, 1166, 1182, 1262, 1303
DinI GGCGCC 1 cut(s) 177
DpnI GATC 7 cut(s) 300, 597, 603, 730, 807, 1187, 1374
DpnII GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
EaeI YGGCCR 2 cut(s) 81, 1603
Eam1104I CTCTTC 1 cut(s) 1718
EarI CTCTTC 1 cut(s) 1718
EciI GGCGGA 1 cut(s) 467
Eco130I CCWWGG 2 cut(s) 1154, 1485
Eco24I GRGCYC 1 cut(s) 629
Eco31I GGTCTC 1 cut(s) 500
Eco32I GATATC 1 cut(s) 331
Eco47I GGWCC 4 cut(s) 351, 391, 528, 1713
Eco57I CTGAAG 1 cut(s) 1539
EcoO109I RGGNCCY 1 cut(s) 1405
EcoRII CCWGG 5 cut(s) 216, 582, 604, 965, 1363
EcoRV GATATC 1 cut(s) 331
EcoT14I CCWWGG 2 cut(s) 1154, 1485
EcoT22I ATGCAT 1 cut(s) 748
EcoT38I GRGCYC 1 cut(s) 629
EgeI GGCGCC 1 cut(s) 177
EheI GGCGCC 1 cut(s) 177
ErhI CCWWGG 2 cut(s) 1154, 1485
Esp3I CGTCTC 2 cut(s) 108, 425
FaeI CATG 9 cut(s) 551, 575, 602, 674, 776, 1379, 1681, 1720, 1764
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FaqI GGGAC 3 cut(s) 364, 883, 1399
FatI CATG 9 cut(s) 547, 571, 598, 670, 772, 1375, 1677, 1716, 1760
FauI CCCGC 2 cut(s) 214, 919
FauNDI CATATG 1 cut(s) 855
FbaI TGATCA 1 cut(s) 1372
FokI GGATG 2 cut(s) 1172, 1777
FriOI GRGCYC 1 cut(s) 629
FspBI CTAG 5 cut(s) 302, 593, 726, 755, 899
GlaI GCGC 5 cut(s) 126, 177, 293, 295, 1610
GsaI CCCAGC 1 cut(s) 428
HaeII RGCGCY 3 cut(s) 128, 179, 1612
HaeIII GGCC 6 cut(s) 83, 627, 818, 1407, 1605, 1622
HapII CCGG 5 cut(s) 128, 179, 394, 531, 629
HgaI GACGC 2 cut(s) 108, 159
HhaI GCGC 5 cut(s) 127, 178, 294, 296, 1611
Hin1I GRCGYC 3 cut(s) 5, 119, 176
Hin1II CATG 9 cut(s) 551, 575, 602, 674, 776, 1379, 1681, 1720, 1764
Hin6I GCGC 5 cut(s) 125, 176, 292, 294, 1609
HinP1I GCGC 5 cut(s) 125, 176, 292, 294, 1609
HinfI GANTC 5 cut(s) 319, 335, 704, 1180, 1284
HpaII CCGG 5 cut(s) 128, 179, 394, 531, 629
HphI GGTGA 7 cut(s) 38, 259, 418, 427, 898, 1382, 1705
Hpy166II GTNNAC 2 cut(s) 444, 1243
Hpy188I TCNGA 7 cut(s) 51, 1084, 1096, 1185, 1493, 1774, 1789
Hpy188III TCNNGA 7 cut(s) 201, 599, 726, 929, 1055, 1189, 1376
Hpy8I GTNNAC 2 cut(s) 444, 1243
Hpy99I CGWCG 3 cut(s) 100, 157, 175
HpyAV CCTTC 6 cut(s) 37, 694, 1126, 1274, 1396, 1418
HpyCH4III ACNGT 8 cut(s) 355, 511, 878, 1177, 1312, 1336, 1360, 1713
HpyCH4IV ACGT 4 cut(s) 237, 1239, 1454, 1689
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 302, 911, 1116, 1133, 1337, 1346, 1580
HpyF3I CTNAG 8 cut(s) 199, 288, 387, 611, 1166, 1182, 1262, 1303
HpySE526I ACGT 4 cut(s) 237, 1239, 1454, 1689
Hsp92I GRCGYC 3 cut(s) 5, 119, 176
Hsp92II CATG 9 cut(s) 551, 575, 602, 674, 776, 1379, 1681, 1720, 1764
HspAI GCGC 5 cut(s) 125, 176, 292, 294, 1609
KasI GGCGCC 1 cut(s) 175
KroI GCCGGC 2 cut(s) 127, 178
KroNI GCCGGC 2 cut(s) 129, 180
Ksp22I TGATCA 1 cut(s) 1372
Kzo9I GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
LmnI GCTCC 4 cut(s) 271, 481, 1275, 1746
Lsp1109I GCAGC 4 cut(s) 538, 541, 668, 745
LweI GCATC 3 cut(s) 760, 1057, 1755
MaeI CTAG 5 cut(s) 302, 593, 726, 755, 899
MaeII ACGT 4 cut(s) 237, 1239, 1454, 1689
MaeIII GTNAC 6 cut(s) 247, 511, 999, 1177, 1306, 1633
MalI GATC 7 cut(s) 300, 597, 603, 730, 807, 1187, 1374
MbiI CCGCTC 1 cut(s) 1749
MboI GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
MfeI CAATTG 3 cut(s) 12, 812, 915
MflI RGATCY 2 cut(s) 298, 805
MhlI GDGCHC 2 cut(s) 465, 629
MlsI TGGCCA 1 cut(s) 1605
MluNI TGGCCA 1 cut(s) 1605
Mly113I GGCGCC 1 cut(s) 176
MlyI GAGTC 1 cut(s) 1174
MmeI TCCRAC 4 cut(s) 168, 219, 696, 1779
Mox20I TGGCCA 1 cut(s) 1605
Mph1103I ATGCAT 1 cut(s) 748
MroNI GCCGGC 2 cut(s) 127, 178
MscI TGGCCA 1 cut(s) 1605
MseI TTAA 2 cut(s) 477, 1023
MslI CAYNNNNRTG 2 cut(s) 468, 1568
Msp20I TGGCCA 1 cut(s) 1605
MspI CCGG 5 cut(s) 128, 179, 394, 531, 629
MspR9I CCNGG 8 cut(s) 218, 395, 532, 584, 606, 629, 967, 1365
MunI CAATTG 3 cut(s) 12, 812, 915
Mva1269I GAATGC 2 cut(s) 748, 1574
MvaI CCWGG 5 cut(s) 218, 584, 606, 967, 1365
MvnI CGCG 3 cut(s) 170, 294, 296
MwoI GCNNNNNNNGC 8 cut(s) 293, 302, 911, 1116, 1133, 1337, 1346, 1580
NaeI GCCGGC 2 cut(s) 129, 180
NarI GGCGCC 1 cut(s) 176
NciI CCSGG 3 cut(s) 395, 532, 629
NdeI CATATG 1 cut(s) 855
NdeII GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
NgoMIV GCCGGC 2 cut(s) 127, 178
NlaIII CATG 9 cut(s) 551, 575, 602, 674, 776, 1379, 1681, 1720, 1764
NlaIV GGNNCC 7 cut(s) 177, 352, 464, 627, 807, 1675, 1753
NmeAIII GCCGAG 4 cut(s) 90, 109, 303, 1650
NmuCI GTSAC 3 cut(s) 247, 1177, 1306
NsiI ATGCAT 1 cut(s) 748
NspI RCATGY 3 cut(s) 551, 674, 776
NspV TTCGAA 1 cut(s) 497
OliI CACNNNNGTG 1 cut(s) 1568
PaeI GCATGC 2 cut(s) 551, 776
PagI TCATGA 2 cut(s) 598, 1375
PaqCI CACCTGC 1 cut(s) 235
PauI GCGCGC 1 cut(s) 292
PcsI WCGNNNNNNNCGW 5 cut(s) 98, 104, 155, 330, 1389
PctI GAATGC 2 cut(s) 748, 1574
PdiI GCCGGC 2 cut(s) 129, 180
PfeI GAWTC 4 cut(s) 319, 335, 704, 1284
PflFI GACNNNGTC 1 cut(s) 1711
PflMI CCANNNNNTGG 1 cut(s) 1761
PleI GAGTC 1 cut(s) 1174
PluTI GGCGCC 1 cut(s) 179
PpsI GAGTC 1 cut(s) 1174
Ppu21I YACGTR 2 cut(s) 238, 1690
PshAI GACNNNNGTC 1 cut(s) 1313
Psp1406I AACGTT 1 cut(s) 1239
Psp6I CCWGG 5 cut(s) 216, 582, 604, 965, 1363
PspFI CCCAGC 1 cut(s) 424
PspGI CCWGG 5 cut(s) 216, 582, 604, 965, 1363
PspN4I GGNNCC 7 cut(s) 177, 352, 464, 627, 807, 1675, 1753
PspOMI GGGCCC 1 cut(s) 625
PspPI GGNCC 9 cut(s) 351, 391, 528, 625, 626, 817, 1405, 1620, 1713
PstNI CAGNNNCTG 1 cut(s) 1310
PsuI RGATCY 2 cut(s) 298, 805
PsyI GACNNNGTC 1 cut(s) 1711
PteI GCGCGC 1 cut(s) 292
RsaI GTAC 2 cut(s) 236, 588
RsaNI GTAC 2 cut(s) 235, 587
RseI CAYNNNNRTG 2 cut(s) 468, 1568
SaqAI TTAA 2 cut(s) 477, 1023
Sau3AI GATC 7 cut(s) 298, 595, 601, 728, 805, 1185, 1372
Sau96I GGNCC 9 cut(s) 351, 391, 528, 625, 626, 817, 1405, 1620, 1713
SchI GAGTC 1 cut(s) 1174
ScrFI CCNGG 8 cut(s) 218, 395, 532, 584, 606, 629, 967, 1365
SduI GDGCHC 2 cut(s) 465, 629
SfaNI GCATC 3 cut(s) 760, 1057, 1755
SfoI GGCGCC 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 497
SinI GGWCC 4 cut(s) 351, 391, 528, 1713
SmiMI CAYNNNNRTG 2 cut(s) 468, 1568
SphI GCATGC 2 cut(s) 551, 776
SspDI GGCGCC 1 cut(s) 175
SspI AATATT 1 cut(s) 1249
SspMI CTAG 5 cut(s) 302, 593, 726, 755, 899
StyD4I CCNGG 8 cut(s) 216, 393, 530, 582, 604, 627, 965, 1363
StyI CCWWGG 2 cut(s) 1154, 1485
TaaI ACNGT 8 cut(s) 355, 511, 878, 1177, 1312, 1336, 1360, 1713
TaiI ACGT 4 cut(s) 240, 1242, 1457, 1692
TaqI TCGA 5 cut(s) 333, 497, 537, 698, 1794
TaqII GACCGA 1 cut(s) 1812
TauI GCSGC 8 cut(s) 259, 287, 308, 401, 452, 1349, 1625, 1664
TfiI GAWTC 4 cut(s) 319, 335, 704, 1284
Tru1I TTAA 2 cut(s) 477, 1023
Tru9I TTAA 2 cut(s) 477, 1023
TscAI CASTG 3 cut(s) 627, 1035, 1311
TseFI GTSAC 3 cut(s) 247, 1177, 1306
TseI GCWGC 4 cut(s) 551, 554, 656, 758
Tsp45I GTSAC 3 cut(s) 247, 1177, 1306
TspDTI ATGAA 5 cut(s) 702, 1155, 1176, 1417, 1567
TspGWI ACGGA 4 cut(s) 47, 96, 284, 392
TspRI CASTG 3 cut(s) 627, 1035, 1311
Tth111I GACNNNGTC 1 cut(s) 1711
Van91I CCANNNNNTGG 1 cut(s) 1761
VpaK11BI GGWCC 4 cut(s) 351, 391, 528, 1713
XapI RAATTY 4 cut(s) 634, 720, 935, 1800
XbaI TCTAGA 1 cut(s) 725
XceI RCATGY 3 cut(s) 551, 674, 776
XspI CTAG 5 cut(s) 302, 593, 726, 755, 899
Zsp2I ATGCAT 1 cut(s) 748
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.