MD01G1121400.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0005782
Physical Location & Seq
Forward (+)
23503348 .. 23510954
7607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1121400.v1.1.491

Sequence Viewer

Length: 4221 bp
ATGATGATAAATCGGGGACTGTTCGGGTGGTCCCCGCCGCACATACAGCCCTTGACGCCGGTCTCAGAGGTGTCGGAGCCGCCAGAGTCGCCTTCGCCGTATATGGAGCAGAGTAACGACGCGTCGGCTCAGCCGATGGAGCAGGAGGAGGAGATGGAGGAGCAGGAGGAGATGGAGCCGCCGCCAGCTGCCGTGCCCTTCTCGAAGCTGTTCACCTGCGCTGACCGGCTCGATTGGGTTCTGATGACTGTTGGGTCGCTTGCCGCGGCGGCTCACGGGACGGCTCTGGTGGTGTACTTGCACTACTTCGCTAAGATTATTCATATACTCTGGGTCGAGCGTGACCACAAGGGCGAGCCGCCGCCAATGAACGACGAGCAGTTCCAGAAGTTTATGGATCTTTCTTTGTCCATCGTTTATATTGCCGCTGGTGTTTTTGCTGCTGGTTGGATTGAGGTCTCATGCTGGATTCTGACGGGAGAACGCCAGACTGCTGTCATCAGGTCAAACTATGTTCAAGTATTACTTAACCAGGATATGAGTTTTTTTGATACCTATGGGAACAACGGGGACATTGTGAGCCAAGTATTGAGTGATGTGTTGCTCATTCAGTCTGCTCTTAGTGAAAAAGTTGGAAATTATATTCATAACATGGCTACATTTTTCAGTGGTCTCATTATTGGATTCATCAACTGTTGGCAGATTGCGGCTATAACATTAGCCACCGGTCCTTTTATTGTGGCTGCTGGAGGAATATCAAATATATTTCTTCATAGGCTTGCTGAGAATATTCAAGATGCATATGCTGAAGCAGCTAGCATTGCTGAACAGGCAGTCTCTTACATTAGGACGTTATATGCATTTACAAATGAAACATTGGCCAAGTATTCGTATGCTACATCACTGCAAGCAACTCTAAGATATGGCATATTGATAAGTCTTGTGCAAGGACTTGGACTTGGATTCACTTATGGCCTTGCCATTTGTTCTTGTGCCCTGCAACTGTGGGTTGGAAGGTTCCTGGTTACTAGTCATGAAGCTCATGGTGGTGAAATTATAACAGCCCTTTTTGCTGTAATATTAAGTGGCCTTGGGCTGAATCAAGCGGCAACAAACTTCTACTCATTTGACCAAGGTCGAATTGCTGCTTATAGACTTTTTGAGATGATAAGTCGATCATCCTCTACTGTTAATCACGAGGGAAGTGCCCTAGCGACTGTACAAGGAAATATTGAGTTTCGAAATGTATATTTCAGTTATCTATCTCGTCCTGAGATCCCCATATTGAGTGGTTTTTACCTCACTGTACCTGCTAAGAAAGCTGTAGCACTCGTTGGCAGAAATGGTTCAGGAAAGAGCAGTATTATCCCCCTCATGGAGCGGTTTTATGATCCAACTTTAGGAGAAGTTCTTTTGGATGGAGAAAATATCAAAAACCTGAGACTGGAGTGGCTAAGAAGTCAGATAGGGCTAGTCACACAGGAGCCTGCTTTGCTGAGTTTAAGTATAAGAGACAATATTGCTTATGGGCGGGATGCTACAATGGACCAAATTGAAGAAGCTGCTAAAATAGCGCATGCGCATACATTTATTACCTCACTTGAGGGAGGATACGATACACAGGTGGGAAGAGCAGGCTTAGCATTGACGGAAGAGCAAAAAATAAAACTTTCAATTGCCAGAGCAGTACTTCTGAACCCCTCAATTCTTTTGCTTGATGAGGTCACTGGTGGACTTGATTTTGAAGCTGAAAAAGCCGTTCAAGAAGCTCTAGATGTCCTTATGTTGGGGCGTTCAACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGCCAACTAGTTGAAATGGGTACCCATGATGAGTTATTAAACCTGGATGGCCTCTATGCGGAGCTTCTCAAAAATGAAGAAGCTGCAAAACTTCCTAGGAGGATGCCAGTGAGCTACAAGGAAACTGCAACTTTCCAAATTGAAAAGGATTCTTTGGCAAGTAATAGCTTCCAAGAACCATCATCTCCGAAAATGATGAAATCACCCTCCCTTCAGAGAACTACTGGCATGTTCCGGATGGGTGATAACACTTTTAACTCACAGGAGTCACCAAAAGCTAAGAGCCCACCAGCAGAGAAAGTGTTGGAAAATGGTCAGGCCTTGGATTCAGAAGATAAGGAACCATCAATAAAAAGGCAGGATAGTTTTGAAATGAGACTACCAGAGTTACCCAAGATTGATGTCCATTCTGCTAATCACCAAACGTCAAATGGTTCGGACCCTGAATCCCCCGTTTCACCCCTTCTGACATCTGATCCGAAAAATGAGCGGTCCCATTCACAAACCTTTAGCCGCCCCCATAGTCATTCAGATGACTTTCCCATGAAAGTGAATGAAGCAAAATGTAGAAATTATAAGAAGGCACCATCATTTTGGAGGCTTGCACAACTTAGTTTTGCTGAATGGCTGTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCTTTCAATCCTCTTCTTGCTTATGTAATCGCACTGATAGTAACAGCATACTACAGAGTTGATGAAGGTCGTCACTTGAAACCGGAAGTAGACAAATGGTGCTTGCTCATTGCTTGTATGGGTATAGTGACAGTCGTTGCCAATTTCTTGCAGCATTTCTACTTTGGTATAATGGGTGAGAAAATGACTGAACGAGTTCGTAGAATGATGTTTTCAGCAATGCTACGAAATGAAGCAGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACCTTATCCATGCGCTTGGCCAATGATGCCACATTTGTACGAGCTGCTTTCAGCAATCGGCTGTCAATATTTATACAGGATAGTGCTGCTGTTGTTGTTGCTCTTCTTATCGGGGTGCTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCCGTTCTCACTATTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGAATTCAGGAGATGCACAGGAAGGCATCTTTGGTTCTTGAGGATGCAGTTAGAAACATTTACACTGTTGTAGCATTCTGTGCTGGTAATAAGGTAATGGAGCTCTACAGGTTGCAGCTAAAGAAAATATTCAAGCAGAGTTTCTTTCATGGAATGGCCATTGGCTTTGCATTTGGCTTTTCGCAGTTCCTTCTTTTTGCCTGCAATGCCCTTCTCCTCTGGCACACTGCACGCACTGTGAAAAATAAGCATATGGATCTACCTACTGCTCTCAAGGAGTATATGGTTTTCTCTTTTGCTACATTTGCACTTGTAGAGCCTTTTGGGCTGGCTCCTTACATCCTGAAGCGCCGAAAATCTCTCATTTCAGTTTTTGAAATCATAGATCGAGTGCCGAAGATTGAGCCAGATGAAAACTCAGCGATGAAACCACCTAATGTTTATGGAAGCATTGAGTTGAAAAATGTTGACTTCTGTTATCCAACCCGCCCAGAACTGTTGGTACTGAGCAATTTCAGTCTCAAAGTAAATGGGGGGCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCAGGAAAGAGCACTATAATTTCTTTGATTGAGAGATTTTATGATCCAGTTGCTGGTCAAGTCTTACTGGATGGTCGAGATCTGAAAGTTTATAATTTGAGATGGTTGAGGAACCACCTAGGTCTTGTTCAGCAGGAACCCATTATATTCTCGACAACCATTCGGGAAAATATTATATATGCAAGGCACAATGCTAGTGAGGCTGAGATGAAAGAGGCAGCCAGAATAGCAAATGCTCACCATTTCATCAGCAGCTTGCCTCATGGTTATGATACGCATGTGGGGATGAGAGGTGTTGACTTGACCCCGGGACAGAAACAGAGAATTGCAATAGCACGCGTCGTGCTAAAGAACGCCCCCATCTTACTATTGGATGAAGCAAGCTCGTCCATTGAATCTGAATCTAGTCGAGTGGTGCAGGAGGCTCTTGATACATTAATCATGGGGAACAAAACAACCATTTTGATAGCCCATAGAGCTGCAATGATGAGGCATGTCGATAACATTGTAGTTCTCAACGGAGGGAGAATTGTGGAAGAAGGGTCCCATGATAATTTAATGTCAAAGAATGGTCTCTATGTCCGTTTGATGCAACCACACTTTGGCAAGGGTTTGCGTCAGCGTCGCCTCGTTTAG

Protein Analysis

1407

Amino Acids

156.23

Weight (kDa)

6.06

Isoelectric Point (pI)

48.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 86 - 361 1.1e-41 ABC transporter transmembrane region
ABC_tran PF00005 431 - 576 4e-22 ABC transporter
ABC_membrane PF00664 838 - 1101 1.9e-38 ABC transporter transmembrane region
ABC_tran PF00005 1175 - 1323 9.7e-29 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1058, 1829, 2439, 3678
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 253
Acc16I TGCGCA 1 cut(s) 1581
Acc36I ACCTGC 2 cut(s) 224, 1318
Acc65I GGTACC 1 cut(s) 1883
AccB1I GGYRCC 2 cut(s) 1883, 2446
AccB7I CCANNNNNTGG 2 cut(s) 3638, 4187
AccBSI CCGCTC 2 cut(s) 1381, 2353
AccI GTMKAC 1 cut(s) 2622
AccII CGCG 3 cut(s) 122, 266, 3924
AccIII TCCGGA 1 cut(s) 2097
AclWI GGATC 6 cut(s) 405, 1270, 1385, 2333, 3282, 3623
AcoI YGGCCR 3 cut(s) 879, 2820, 3174
AcsI RAATTY 1 cut(s) 3021
AcuI CTGAAG 3 cut(s) 828, 2060, 3383
AcyI GRCGYC 1 cut(s) 56
AfaI GTAC 7 cut(s) 296, 1221, 1308, 1689, 1885, 2841, 3522
AfiI CCNNNNNNNGG 7 cut(s) 1375, 1400, 1444, 1786, 1921, 3638, 4187
AflIII ACRYGT 2 cut(s) 120, 3922
AgeI ACCGGT 1 cut(s) 725
AhlI ACTAGT 2 cut(s) 1028, 1870
AjnI CCWGG 3 cut(s) 531, 1020, 1905
AjuI GAANNNNNNNTTGG 2 cut(s) 3032, 3064
Alw21I GWGCWC 2 cut(s) 3123, 3599
Alw26I GTCTC 9 cut(s) 67, 463, 677, 841, 1435, 1506, 2233, 3542, 4163
AlwI GGATC 6 cut(s) 405, 1270, 1385, 2333, 3282, 3623
AlwNI CAGNNNCTG 1 cut(s) 3638
Ama87I CYCGRG 1 cut(s) 3892
Aor13HI TCCGGA 1 cut(s) 2097
AoxI GGCC 8 cut(s) 879, 973, 1087, 1864, 1912, 2181, 2820, 3174
ApoI RAATTY 1 cut(s) 3021
AseI ATTAAT 1 cut(s) 4022
AsiGI ACCGGT 1 cut(s) 725
Asp700I GAANNNNTTC 4 cut(s) 209, 1345, 2727, 3146
Asp718I GGTACC 1 cut(s) 1883
AspA2I CCTAGG 2 cut(s) 1958, 3703
AspLEI GCGC 5 cut(s) 221, 1576, 1582, 2817, 3369
AspS9I GGNCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
AsuC2I CCSGG 2 cut(s) 3893, 3894
AsuHPI GGTGA 9 cut(s) 205, 1061, 2058, 2117, 2124, 2273, 2313, 2720, 3815
AsuII TTCGAA 1 cut(s) 1240
AsuNHI GCTAGC 1 cut(s) 815
AvaI CYCGRG 1 cut(s) 3892
AvaII GGWCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
AvrII CCTAGG 2 cut(s) 1958, 3703
BaeGI GKGCMC 3 cut(s) 198, 997, 1210
BaeI ACNNNNGTAYC 4 cut(s) 1608, 1641, 3849, 3882
BalI TGGCCA 3 cut(s) 881, 2822, 3176
BanI GGYRCC 2 cut(s) 1883, 2446
BanII GRGCYC 2 cut(s) 2150, 3123
BauI CACGAG 1 cut(s) 1196
Bbv12I GWGCWC 2 cut(s) 3123, 3599
BceAI ACGGC 4 cut(s) 82, 176, 297, 1742
BcgI CGANNNNNNTGC 2 cut(s) 1187, 1221
BciT130I CCWGG 3 cut(s) 533, 1022, 1907
BciVI GTATCC 1 cut(s) 1604
BcnI CCSGG 2 cut(s) 3893, 3894
BcoDI GTCTC 9 cut(s) 67, 463, 677, 841, 1435, 1506, 2233, 3542, 4163
BcuI ACTAGT 2 cut(s) 1028, 1870
BfmI CTRYAG 4 cut(s) 1323, 2584, 3124, 3561
BfoI RGCGCY 1 cut(s) 3370
BfuAI ACCTGC 2 cut(s) 224, 1318
BfuI GTATCC 1 cut(s) 1604
BglI GCCNNNNNGGC 2 cut(s) 269, 3343
BglII AGATCT 1 cut(s) 3664
BlnI CCTAGG 2 cut(s) 1958, 3703
BlpI GCTNAGC 2 cut(s) 129, 1639
BmcAI AGTACT 1 cut(s) 1689
Bme1390I CCNGG 5 cut(s) 533, 1022, 1907, 3893, 3894
Bme18I GGWCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
BmeT110I CYCGRG 1 cut(s) 3892
BmgT120I GGNCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
BmrFI CCNGG 5 cut(s) 533, 1022, 1907, 3893, 3894
BmsI GCATC 8 cut(s) 787, 1525, 1956, 2818, 3021, 3052, 3053, 4164
BmtI GCTAGC 1 cut(s) 819
BoxI GACNNNNGTC 3 cut(s) 59, 494, 1134
BpmI CTGGAG 2 cut(s) 768, 1466
Bpu1102I GCTNAGC 2 cut(s) 129, 1639
Bpu14I TTCGAA 1 cut(s) 1240
BpuEI CTTGAG 3 cut(s) 1622, 3077, 3275
BpuMI CCSGG 2 cut(s) 3893, 3894
BsaHI GRCGYC 1 cut(s) 56
BsaI GGTCTC 4 cut(s) 67, 463, 677, 4163
BsaJI CCNNGG 8 cut(s) 264, 1090, 1132, 1958, 2184, 3703, 3891, 3892
BsaWI WCCGGW 3 cut(s) 725, 2097, 2614
Bsc4I CCNNNNNNNGG 7 cut(s) 1375, 1400, 1444, 1786, 1921, 3638, 4187
Bse118I RCCGGY 3 cut(s) 58, 225, 725
Bse1I ACTGG 6 cut(s) 1449, 1732, 1970, 2092, 3632, 3657
Bse3DI GCAATG 6 cut(s) 819, 2640, 2757, 2982, 3229, 4074
BseAI TCCGGA 1 cut(s) 2097
BseBI CCWGG 3 cut(s) 533, 1022, 1907
BseDI CCNNGG 8 cut(s) 264, 1090, 1132, 1958, 2184, 3703, 3891, 3892
BseLI CCNNNNNNNGG 7 cut(s) 1375, 1400, 1444, 1786, 1921, 3638, 4187
BseMI GCAATG 6 cut(s) 819, 2640, 2757, 2982, 3229, 4074
BseMII CTCAG 9 cut(s) 78, 143, 774, 1263, 1430, 1487, 3450, 3515, 3780
BseNI ACTGG 6 cut(s) 1449, 1732, 1970, 2092, 3632, 3657
BseRI GAGGAG 5 cut(s) 161, 164, 173, 182, 3224
BseSI GKGCMC 3 cut(s) 198, 997, 1210
BsgI GTGCAG 2 cut(s) 3231, 4022
Bsh1236I CGCG 3 cut(s) 122, 266, 3924
BshFI GGCC 8 cut(s) 881, 975, 1089, 1866, 1914, 2183, 2822, 3176
BshNI GGYRCC 2 cut(s) 1883, 2446
BshTI ACCGGT 1 cut(s) 725
BsiHKAI GWGCWC 2 cut(s) 3123, 3599
BsiHKCI CYCGRG 1 cut(s) 3892
BsiSI CCGG 6 cut(s) 59, 226, 726, 2098, 2615, 3893
BslFI GGGAC 7 cut(s) 16, 30, 292, 584, 2341, 3909, 4114
BslI CCNNNNNNNGG 7 cut(s) 1375, 1400, 1444, 1786, 1921, 3638, 4187
BsmAI GTCTC 9 cut(s) 67, 463, 677, 841, 1435, 1506, 2233, 3542, 4163
BsmFI GGGAC 7 cut(s) 16, 30, 292, 584, 2341, 3909, 4114
BsmI GAATGC 2 cut(s) 1840, 3092
BsnI GGCC 8 cut(s) 881, 975, 1089, 1866, 1914, 2183, 2822, 3176
Bso31I GGTCTC 4 cut(s) 67, 463, 677, 4163
BsoBI CYCGRG 1 cut(s) 3892
Bsp119I TTCGAA 1 cut(s) 1240
Bsp1286I GDGCHC 6 cut(s) 198, 997, 1210, 2150, 3123, 3599
Bsp13I TCCGGA 1 cut(s) 2097
Bsp1407I TGTACA 1 cut(s) 1219
Bsp143I GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
Bsp1720I GCTNAGC 2 cut(s) 129, 1639
BspANI GGCC 8 cut(s) 881, 975, 1089, 1866, 1914, 2183, 2822, 3176
BspCNI CTCAG 9 cut(s) 77, 142, 775, 1264, 1431, 1488, 3449, 3516, 3781
BspEI TCCGGA 1 cut(s) 2097
BspFNI CGCG 3 cut(s) 122, 266, 3924
BspHI TCATGA 1 cut(s) 1033
BspMI ACCTGC 2 cut(s) 224, 1318
BspOI GCTAGC 1 cut(s) 819
BspPI GGATC 6 cut(s) 405, 1270, 1385, 2333, 3282, 3623
BspQI GCTCTTC 3 cut(s) 1624, 1647, 2910
BspT104I TTCGAA 1 cut(s) 1240
BspT107I GGYRCC 2 cut(s) 1883, 2446
BspTNI GGTCTC 4 cut(s) 67, 463, 677, 4163
BsrBI CCGCTC 2 cut(s) 1381, 2353
BsrDI GCAATG 6 cut(s) 819, 2640, 2757, 2982, 3229, 4074
BsrFI RCCGGY 3 cut(s) 58, 225, 725
BsrGI TGTACA 1 cut(s) 1219
BsrI ACTGG 6 cut(s) 1449, 1732, 1970, 2092, 3632, 3657
BssAI RCCGGY 3 cut(s) 58, 225, 725
BssECI CCNNGG 8 cut(s) 264, 1090, 1132, 1958, 2184, 3703, 3891, 3892
BssMI GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
BssNI GRCGYC 1 cut(s) 56
BssSI CACGAG 1 cut(s) 1196
BssT1I CCWWGG 5 cut(s) 1090, 1132, 1958, 2184, 3703
Bst2BI CACGAG 1 cut(s) 1196
Bst2UI CCWGG 3 cut(s) 533, 1022, 1907
Bst6I CTCTTC 5 cut(s) 1624, 1647, 2550, 2778, 2910
BstACI GRCGYC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 3098
BstAUI TGTACA 1 cut(s) 1219
BstBI TTCGAA 1 cut(s) 1240
BstDSI CCRYGG 1 cut(s) 264
BstFNI CGCG 3 cut(s) 122, 266, 3924
BstH2I RGCGCY 1 cut(s) 3370
BstHHI GCGC 5 cut(s) 221, 1576, 1582, 2817, 3369
BstKTI GATC 9 cut(s) 400, 1178, 1278, 1393, 2341, 3277, 3406, 3631, 3667
BstMAI GTCTC 9 cut(s) 67, 463, 677, 841, 1435, 1506, 2233, 3542, 4163
BstMBI GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
BstNI CCWGG 3 cut(s) 533, 1022, 1907
BstNSI RCATGY 4 cut(s) 1580, 2095, 3866, 4082
BstPAI GACNNNNGTC 3 cut(s) 59, 494, 1134
BstSCI CCNGG 5 cut(s) 531, 1020, 1905, 3891, 3892
BstSFI CTRYAG 4 cut(s) 1323, 2584, 3124, 3561
BstSLI GKGCMC 3 cut(s) 198, 997, 1210
BstUI CGCG 3 cut(s) 122, 266, 3924
BstX2I RGATCY 4 cut(s) 397, 1275, 3274, 3664
BstXI CCANNNNNNTGG 2 cut(s) 2457, 2818
BstYI RGATCY 4 cut(s) 397, 1275, 3274, 3664
BsuI GTATCC 1 cut(s) 1604
BsuRI GGCC 8 cut(s) 881, 975, 1089, 1866, 1914, 2183, 2822, 3176
BtgI CCRYGG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 3455
BtsI GCAGTG 2 cut(s) 902, 3243
BveI ACCTGC 2 cut(s) 224, 1318
CaiI CAGNNNCTG 1 cut(s) 3638
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 5 cut(s) 221, 1576, 1582, 2817, 3369
Cfr10I RCCGGY 3 cut(s) 58, 225, 725
Cfr13I GGNCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
Cfr42I CCGCGG 1 cut(s) 267
Cfr9I CCCGGG 1 cut(s) 3892
CseI GACGC 6 cut(s) 64, 111, 128, 3913, 4190, 4196
Csp6I GTAC 7 cut(s) 295, 1220, 1307, 1688, 1884, 2840, 3521
CspAI ACCGGT 1 cut(s) 725
CviQI GTAC 7 cut(s) 295, 1220, 1307, 1688, 1884, 2840, 3521
DpnI GATC 9 cut(s) 399, 1177, 1277, 1392, 2340, 3276, 3405, 3630, 3666
DpnII GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 3 cut(s) 879, 2820, 3174
Eam1104I CTCTTC 5 cut(s) 1624, 1647, 2550, 2778, 2910
EarI CTCTTC 5 cut(s) 1624, 1647, 2550, 2778, 2910
Ecl136II GAGCTC 1 cut(s) 3121
Eco130I CCWWGG 5 cut(s) 1090, 1132, 1958, 2184, 3703
Eco147I AGGCCT 1 cut(s) 2183
Eco24I GRGCYC 2 cut(s) 2150, 3123
Eco31I GGTCTC 4 cut(s) 67, 463, 677, 4163
Eco47I GGWCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
Eco53kI GAGCTC 1 cut(s) 3121
Eco57I CTGAAG 3 cut(s) 828, 2060, 3383
Eco88I CYCGRG 1 cut(s) 3892
EcoICRI GAGCTC 1 cut(s) 3121
EcoO109I RGGNCCY 1 cut(s) 4128
EcoRI GAATTC 1 cut(s) 3021
EcoRII CCWGG 3 cut(s) 531, 1020, 1905
EcoT14I CCWWGG 5 cut(s) 1090, 1132, 1958, 2184, 3703
EcoT22I ATGCAT 2 cut(s) 802, 862
EcoT38I GRGCYC 2 cut(s) 2150, 3123
ErhI CCWWGG 5 cut(s) 1090, 1132, 1958, 2184, 3703
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 7 cut(s) 16, 30, 292, 584, 2341, 3909, 4114
FauI CCCGC 3 cut(s) 42, 1524, 3512
FauNDI CATATG 2 cut(s) 802, 3270
FblI GTMKAC 1 cut(s) 2622
FriOI GRGCYC 2 cut(s) 2150, 3123
FspAI RTGCGCAY 1 cut(s) 1581
FspI TGCGCA 1 cut(s) 1581
GlaI GCGC 5 cut(s) 220, 1575, 1581, 2816, 3368
GsuI CTGGAG 2 cut(s) 768, 1466
HaeII RGCGCY 1 cut(s) 3370
HaeIII GGCC 8 cut(s) 881, 975, 1089, 1866, 1914, 2183, 2822, 3176
HapII CCGG 6 cut(s) 59, 226, 726, 2098, 2615, 3893
HgaI GACGC 6 cut(s) 64, 111, 128, 3913, 4190, 4196
HhaI GCGC 5 cut(s) 221, 1576, 1582, 2817, 3369
Hin1I GRCGYC 1 cut(s) 56
Hin6I GCGC 5 cut(s) 219, 1574, 1580, 2815, 3367
HinP1I GCGC 5 cut(s) 219, 1574, 1580, 2815, 3367
HincII GTYRAC 2 cut(s) 3487, 3883
HindII GTYRAC 2 cut(s) 3487, 3883
HpaII CCGG 6 cut(s) 59, 226, 726, 2098, 2615, 3893
HphI GGTGA 9 cut(s) 205, 1061, 2058, 2117, 2124, 2273, 2313, 2720, 3815
Hpy166II GTNNAC 6 cut(s) 213, 295, 1733, 2623, 3487, 3883
Hpy8I GTNNAC 6 cut(s) 213, 295, 1733, 2623, 3487, 3883
Hpy99I CGWCG 5 cut(s) 122, 127, 377, 3929, 4212
HpyCH4IV ACGT 2 cut(s) 851, 2288
HpySE526I ACGT 2 cut(s) 851, 2288
Hsp92I GRCGYC 1 cut(s) 56
HspAI GCGC 5 cut(s) 219, 1574, 1580, 2815, 3367
KflI GGGWCCC 1 cut(s) 4128
Kpn2I TCCGGA 1 cut(s) 2097
KpnI GGTACC 1 cut(s) 1887
KspI CCGCGG 1 cut(s) 267
Kzo9I GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
LguI GCTCTTC 3 cut(s) 1624, 1647, 2910
LweI GCATC 8 cut(s) 787, 1525, 1956, 2818, 3021, 3052, 3053, 4164
MaeII ACGT 2 cut(s) 851, 2288
MalI GATC 9 cut(s) 399, 1177, 1277, 1392, 2340, 3276, 3405, 3630, 3666
MbiI CCGCTC 2 cut(s) 1381, 2353
MboI GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
MfeI CAATTG 1 cut(s) 1674
MflI RGATCY 4 cut(s) 397, 1275, 3274, 3664
MhlI GDGCHC 6 cut(s) 198, 997, 1210, 2150, 3123, 3599
MlsI TGGCCA 3 cut(s) 881, 2822, 3176
MluI ACGCGT 2 cut(s) 120, 3922
MluNI TGGCCA 3 cut(s) 881, 2822, 3176
MlyI GAGTC 2 cut(s) 95, 2138
MmeI TCCRAC 7 cut(s) 54, 428, 613, 991, 1418, 2148, 3524
Mox20I TGGCCA 3 cut(s) 881, 2822, 3176
Mph1103I ATGCAT 2 cut(s) 802, 862
MroI TCCGGA 1 cut(s) 2097
MroXI GAANNNNTTC 4 cut(s) 209, 1345, 2727, 3146
MscI TGGCCA 3 cut(s) 881, 2822, 3176
MseI TTAA 8 cut(s) 528, 1082, 1191, 1502, 1901, 2118, 4022, 4142
MslI CAYNNNNRTG 5 cut(s) 1047, 1850, 2394, 2411, 3852
Msp20I TGGCCA 3 cut(s) 881, 2822, 3176
MspA1I CMGCKG 3 cut(s) 188, 266, 428
MspI CCGG 6 cut(s) 59, 226, 726, 2098, 2615, 3893
MspR9I CCNGG 5 cut(s) 533, 1022, 1907, 3893, 3894
MunI CAATTG 1 cut(s) 1674
Mva1269I GAATGC 2 cut(s) 1840, 3092
MvaI CCWGG 3 cut(s) 533, 1022, 1907
MvnI CGCG 3 cut(s) 122, 266, 3924
NciI CCSGG 2 cut(s) 3893, 3894
NdeI CATATG 2 cut(s) 802, 3270
NdeII GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
NheI GCTAGC 1 cut(s) 815
NmeAIII GCCGAG 1 cut(s) 1792
NmuCI GTSAC 6 cut(s) 341, 1474, 1723, 2130, 2603, 2659
NsbI TGCGCA 1 cut(s) 1581
NsiI ATGCAT 2 cut(s) 802, 862
NspI RCATGY 4 cut(s) 1580, 2095, 3866, 4082
NspV TTCGAA 1 cut(s) 1240
PaeI GCATGC 1 cut(s) 1580
PagI TCATGA 1 cut(s) 1033
PaqCI CACCTGC 1 cut(s) 224
PceI AGGCCT 1 cut(s) 2183
PciSI GCTCTTC 3 cut(s) 1624, 1647, 2910
PcsI WCGNNNNNNNCGW 2 cut(s) 95, 131
PctI GAATGC 2 cut(s) 1840, 3092
PdmI GAANNNNTTC 4 cut(s) 209, 1345, 2727, 3146
PfeI GAWTC 9 cut(s) 469, 684, 963, 1099, 2012, 2189, 2309, 3980, 3986
PflMI CCANNNNNTGG 2 cut(s) 3638, 4187
PinAI ACCGGT 1 cut(s) 725
PleI GAGTC 2 cut(s) 94, 2137
PpsI GAGTC 2 cut(s) 94, 2137
PpuMI RGGWCCY 1 cut(s) 4128
PshAI GACNNNNGTC 3 cut(s) 59, 494, 1134
PshBI ATTAAT 1 cut(s) 4022
PsiI TTATAA 4 cut(s) 1058, 1829, 2439, 3678
Psp124BI GAGCTC 1 cut(s) 3123
Psp5II RGGWCCY 1 cut(s) 4128
Psp6I CCWGG 3 cut(s) 531, 1020, 1905
PspGI CCWGG 3 cut(s) 531, 1020, 1905
PspPI GGNCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
PspPPI RGGWCCY 1 cut(s) 4128
PsrI GAACNNNNNNTAC 2 cut(s) 3504, 3536
PstNI CAGNNNCTG 1 cut(s) 3638
PsuI RGATCY 4 cut(s) 397, 1275, 3274, 3664
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 7 cut(s) 296, 1221, 1308, 1689, 1885, 2841, 3522
RsaNI GTAC 7 cut(s) 295, 1220, 1307, 1688, 1884, 2840, 3521
RseI CAYNNNNRTG 5 cut(s) 1047, 1850, 2394, 2411, 3852
SacI GAGCTC 1 cut(s) 3123
SacII CCGCGG 1 cut(s) 267
SapI GCTCTTC 3 cut(s) 1624, 1647, 2910
SaqAI TTAA 8 cut(s) 528, 1082, 1191, 1502, 1901, 2118, 4022, 4142
Sau3AI GATC 9 cut(s) 397, 1175, 1275, 1390, 2338, 3274, 3403, 3628, 3664
Sau96I GGNCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
ScaI AGTACT 1 cut(s) 1689
SchI GAGTC 2 cut(s) 95, 2138
ScrFI CCNGG 5 cut(s) 533, 1022, 1907, 3893, 3894
SduI GDGCHC 6 cut(s) 198, 997, 1210, 2150, 3123, 3599
SfaNI GCATC 8 cut(s) 787, 1525, 1956, 2818, 3021, 3052, 3053, 4164
SfcI CTRYAG 4 cut(s) 1323, 2584, 3124, 3561
Sfr303I CCGCGG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 1240
SgrBI CCGCGG 1 cut(s) 267
SinI GGWCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
SmaI CCCGGG 1 cut(s) 3894
SmiMI CAYNNNNRTG 5 cut(s) 1047, 1850, 2394, 2411, 3852
SmlI CTYRAG 3 cut(s) 1601, 3056, 3290
SmoI CTYRAG 3 cut(s) 1601, 3056, 3290
SpeI ACTAGT 2 cut(s) 1028, 1870
SphI GCATGC 1 cut(s) 1580
SseBI AGGCCT 1 cut(s) 2183
SspI AATATT 7 cut(s) 790, 1080, 1231, 1519, 2871, 3147, 3757
SstI GAGCTC 1 cut(s) 3123
StuI AGGCCT 1 cut(s) 2183
StyD4I CCNGG 5 cut(s) 531, 1020, 1905, 3891, 3892
StyI CCWWGG 5 cut(s) 1090, 1132, 1958, 2184, 3703
TaiI ACGT 2 cut(s) 854, 2291
TatI WGTACW 3 cut(s) 294, 1219, 1687
TfiI GAWTC 9 cut(s) 469, 684, 963, 1099, 2012, 2189, 2309, 3980, 3986
Tru1I TTAA 8 cut(s) 528, 1082, 1191, 1502, 1901, 2118, 4022, 4142
Tru9I TTAA 8 cut(s) 528, 1082, 1191, 1502, 1901, 2118, 4022, 4142
TseFI GTSAC 6 cut(s) 341, 1474, 1723, 2130, 2603, 2659
Tsp45I GTSAC 6 cut(s) 341, 1474, 1723, 2130, 2603, 2659
TspGWI ACGGA 3 cut(s) 1664, 4119, 4157
TspMI CCCGGG 1 cut(s) 3892
Van91I CCANNNNNTGG 2 cut(s) 3638, 4187
VpaK11BI GGWCC 6 cut(s) 30, 728, 1546, 2302, 2355, 4128
VspI ATTAAT 1 cut(s) 4022
XapI RAATTY 1 cut(s) 3021
XbaI TCTAGA 1 cut(s) 1771
XceI RCATGY 4 cut(s) 1580, 2095, 3866, 4082
XcmI CCANNNNNNNNNTGG 2 cut(s) 2291, 3952
XmaI CCCGGG 1 cut(s) 3892
XmaJI CCTAGG 2 cut(s) 1958, 3703
XmiI GTMKAC 1 cut(s) 2622
XmnI GAANNNNTTC 4 cut(s) 209, 1345, 2727, 3146
ZrmI AGTACT 1 cut(s) 1689
Zsp2I ATGCAT 2 cut(s) 802, 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.