MD07G1191100.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0005782
Physical Location & Seq
Forward (+)
27103310 .. 27111138
7829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1191100.v1.1.491

Sequence Viewer

Length: 4224 bp
ATGATGATATCTCGGGGGCTGTTCGGGTGGTCCCCGCCCCACGTACAGCCCTTGACACCGGTCTCAGAGGTGTCGGAGCCGCCCGAGTCGCCGTCGCCGTACATGGAGCAGAGTATCGACGCGTCGGCACAGCCGATGGAGCAGGAGGAGGAGATGGATGAGCAGGAGGAGATGGAGCCGCCGCCAGCTGCCGTGCCCTTCTCAAGGCTGTTCACCTGCGCGGACCGGCTCGATTGGGTTCTGATGACTGTTGGGTCGATCGCCGCGGCGGCTCACGGGACGGCTCTGGTGGTGTACTTGCACTACTTCGCTAAGATTATTCATGTACTATGGATCGGCCATAACCCAAAAGGGGAGCCGCCACAAATGAACGACGAACAGTTCCAGAAGTTTATGGATCTTTCTTTGTCCATCATGTATATTGCCGTTGGTGTTTTTGCAGCTGGTTGGATTGAGGTCTCATGCTGGATTCTGACGGGAGAACGGCAGACTGCTGTCATCAGGTCAAAATATGTTCAAGTATTACTTAACCAGGATATGAGTTTTTTTGATACCTATGGGAACAACGGGGACATTGTGAGCCAAGTATTGAGTGATGTGTTGCTCATTCAGTCTGCTCTTAGTGAAAAAGTTGGAAATTATATTCATAACATGGCTACATTTTTCAGTGGTCTGATTATTGGATTCATCAACTGTTGGCAGATCGCGGCTATAACTTTAGCCACCGGTCCTTTTATTGTGGCTGCTGGAGGAATATCAAATATATTTCTTCATAGGCTTGCTGAAAATATTCAAGATGCATATGCTGAAGCAGCTAGCATAGCTGAACAGGCAGTCTCTTACATTAGGACGTTATATGCATTTACGAATGAAACGTTGGCCAAGTATTCGTATGCTACATCACTGCAAGCAACTCTCAGATATGGTATATTGATAAGTCTTGTGCAAGGACTTGGTCTTGGATTCACTTATGGCCTTGCCATTTGTTCTTGTGCCTTGCAACTGTGGGTTGGAAGGTTCCTGGTTAATAGTCATAAAGCTCATGGTGGTGAAATTATAACAGCGCTTTTTGCTGTAATATTAAGTGGCCTTGGGCTGAATCAAGCGGCAACAAACTTCTACTCATTTGATCAAGGTCGAATTGCTGCCTATAGACTTTTTGAGATGATAAGTCGATCATCCTCTACTGTTAATCACGAGGGAAGTTCCCTAGCGACTGTACAAGGAAATATTGAGTTTCGAAATGTATATTTCAGTTATCTATCTCGTCCTGAGATCCCTATATTGAGTGGTTTTTACCTCACCGTGCCTGCTAAGAAAGCTGTGGCACTCGTTGGCAGAAATGGTTCAGGAAAGAGCAGTATTATCCCCCTCATGGAGCGGTTTTATGATCCAACATTAGGAGAAGTTCTTTTGGATGGAGAAAATATCAAAAACCTGAGACTGGAGTGGCTTCGAAGTCAGATAGGGCTAGTCACACAGGAACCTGCTTTGCTGAGTTTAAGTATAAGAGATAATATTGCATATGGGCGGGATGCTACAATGGATCAAATTGAAGAAGCTGCTAAAATAGCTCATGCGCATACATTTATTACCTCACTTGAGGGAGGATACGATACACAGGTTGGAAGAGCAGGCTTAGCATTGACGGAAGAGCAAAAAATAAAACTTTCAATTGCCAGAGCAGTACTTCTGAACCCCTCAATTCTTTTGCTTGATGAGGTCACTGGTGGACTAGATTTTGAAGCTGAAAAAGCTGTTCAAGAAGCTCTAGATCTTCTTATGTTAGGGCGTTCAACTATAATTATAGCTCGGCGGCTTAGTCTTATAAGGAATGCTGATTACATAGCTGTGATGGAGGAAGGTCAACTAGTTGAAATGGGTACCCATGATGAGTTATTAACCCTGGATGGCCTCTATGCGGAGCTTCTCAAAAATGAAGAAGCTGCAAAACTTCCTAGGAGGATGCCAGTGAGGAACTACAAGGAAACTGCAACTTTCCAAATTGAAAAGGACTCTTCGGCAAGTAATAGCTTCCAAGAACCATCATCTCCGGAAATGATGAAATCACCCTCCCTTCAGAGAACTACTGGCATGTTCCGGATGGGTGATAACACTTTTAACTCACAGGAGTCACCAAAAGCTAAGAGCCCACCAGCAGAGAAAGTGTTGGAAAATGGTCAGGCCGTGGATTCAACAGATAAGGAACCATCAATAAAAAGGCAGGATAGTTTTGAAAGGAGACTACCAGAGTTACCCAAGATTGATGTCCAGTCTGCGAATTTCCAAACGTCAAATGGTTCGGACCCTGAATCCCCCGTGTCACCCCTTTTGATATCTGATCCAAAAAATGAGCGTTCCCATTCACAATCCTTTAGCCGCCCTCATAGTCATTCAGATGACTTTCCCATGAAAGCGAACGAAGTAAAAAGTACAAATTATAAGAAGGCACCATCATTTTGGAGACTTGCACAACTTAGTTTTGCTGAGTGGCTGTATGCTGTGTTAGGAAGCATTGGTGCTGCTATCTTTGGTTCCTTCAATCCTCTTCTTGCTTATGTAATCGCACTGATAGTAACATCGTACTATAGAGTTGATGAAGGTCGTCACTTGAGACAGGAAGTAGACAAATGGTGCTTGATCATTGCTTGTATGGGTATAGTGACAGTCGTTGCCAATTTCTTGCAGCATTTCTACTTTGGTATTATGGGTGAGAAAATGACTGAACGGGTTCGTAGAATGATGTTTTCAGCAATGCTGCGAAATGAAGCGGGATGGTTTGATGAAGAGGAAAACAGTGCTGATACCTTATCCATGCGCTTGGCCAACGATGCTACATTTGTACGAGCTGCTTTCAGCAATCGGCTGTCAATATTTATACAGGATAGTGCTGCTGTTATTGTGGCTCTTCTTATTGGGATGCTGCTACAATGGCGATTAGCACTTGTGGCTTTGGCAACCCTACCAGTTCTCACTCTTTCTGCCATTGCACAGAAATTATGGCTTGCTGGTTTTTCAAGGGGAATTCAGGAGATGCACAGGAAGGCATCTTTGGTTCTAGAGGATGCGGTTAGAAACATTTACACTGTTGTAGCATTCTGTGCTGGTAACAAGGTAATGGAGCTCTACAGGTTGCAGCTGAAGAAAATATTCAAGCAGAGTTTCTTTCATGGAATGGCCATTGGATTTGCATTTGGCTTTTCGCAGTTCCTTCTTTTTGCCTGCAATGCCCTTCTCCTCTGGTACACTGCACGCATGGTGAAAAATAAGCATATGGATCTACCTACTGCTATCAAGGAGTATATGGTTTTCTCTTTTGCTACATTTGCACTTGTAGAACCTTTTGGGTTGGCTCCTTACATCCTGAAACGCCGAAAATCTCTCATTTCAGTTTTTGAAATCATAGATCGAGTGCCGAAGATTGAGCCAGATGAAAACTCAGCGATGAAGCCACCTAATGTTTATGGAAGCCTTGAGTTGAAAAATGTTGACTTCTGTTATCCAACTCGCCCAGAACTGTTGGTACTGAGCAATTTCAGTCTCAAAGTAAATGGGGGGCAAACTGTAGCTGTGGTGGGAGTTTCAGGGTCAGGAAAGAGCACTATAATTTCCTTGATTGAGAGATTTTATGATCCAGTTGCTGGTCAAGTCTTACTGGATGGTCGAGATCTGAAAGTTTATAATTTGAGATGGTTGAGGAACCACCTAGGTCTTGTTCAGCAGGAGCCCATTATATTCTCAACAACCATTCGGGAAAATATTATATATGCAAGGCACAATGCTAGTGAGGCCGAGATGAAAGAGGCAGCCAGAATAGCAAATGCTCACCATTTCATCAGCAGCTTGCCTCATGGTTATGATACGCATGTGGGGATGAGAGGTGTTGACTTGACTCCAGGACAGAAACAGAGGATTGCAATAGCACGGGTCGTGCTAAAGAATGCCCCCATCTTATTATTGGATGAAGCAAGCTCGTCCATTGAATCCGAATCAAGTCGAGTGGTTCAGGAGGCTCTTGATACATTAATCATGGGGAACAAAACAACCATTTTGATAGCCCATAGAGCTGCAATGATGAGGCATGTTGACAACATTGTAGTTCTCAACGGAGGGAGAATCGTGGAAGAAGGGTCCCATGATAATTTAATGTCAAAGAATGGTCTCTATGTCCGTTTGATGCAACCGCACTATGGCAAGGGTTTGCGTCAGCATAGACTCGTCTAG

Protein Analysis

1408

Amino Acids

156.45

Weight (kDa)

6.15

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 87 - 361 3.6e-41 ABC transporter transmembrane region
ABC_tran PF00005 431 - 576 4e-22 ABC transporter
ABC_membrane PF00664 839 - 1102 1.7e-39 ABC transporter transmembrane region
ABC_tran PF00005 1176 - 1324 9.7e-29 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 1058, 1829, 2442, 3681
AarI CACCTGC 1 cut(s) 224
AasI GACNNNNNNGTC 1 cut(s) 253
Acc16I TGCGCA 1 cut(s) 1581
Acc36I ACCTGC 2 cut(s) 224, 1495
Acc65I GGTACC 1 cut(s) 1883
AccB1I GGYRCC 2 cut(s) 1883, 2449
AccB7I CCANNNNNTGG 1 cut(s) 3641
AccBSI CCGCTC 1 cut(s) 1381
AccI GTMKAC 1 cut(s) 2625
AccII CGCG 4 cut(s) 122, 221, 266, 707
AccIII TCCGGA 2 cut(s) 2053, 2100
AclI AACGTT 1 cut(s) 875
AclWI GGATC 8 cut(s) 341, 405, 1270, 1385, 1554, 2336, 3285, 3626
AcoI YGGCCR 4 cut(s) 337, 879, 2823, 3177
AcsI RAATTY 2 cut(s) 2281, 3024
AcuI CTGAAG 3 cut(s) 828, 2063, 3161
AfeI AGCGCT 1 cut(s) 1065
AfiI CCNNNNNNNGG 8 cut(s) 204, 352, 1375, 1400, 1444, 1921, 3641, 4190
AflIII ACRYGT 1 cut(s) 120
AgeI ACCGGT 2 cut(s) 58, 725
AhlI ACTAGT 1 cut(s) 1870
AjnI CCWGG 4 cut(s) 531, 1020, 1905, 3895
AjuI GAANNNNNNNTTGG 4 cut(s) 860, 892, 3035, 3067
Alw21I GWGCWC 2 cut(s) 3126, 3602
Alw26I GTCTC 9 cut(s) 67, 463, 841, 1435, 2236, 2458, 2608, 3545, 4166
AlwI GGATC 8 cut(s) 341, 405, 1270, 1385, 1554, 2336, 3285, 3626
AlwNI CAGNNNCTG 1 cut(s) 3641
Ama87I CYCGRG 2 cut(s) 12, 83
Aor13HI TCCGGA 2 cut(s) 2053, 2100
Aor51HI AGCGCT 1 cut(s) 1065
AoxI GGCC 9 cut(s) 337, 879, 973, 1087, 1912, 2184, 2823, 3177, 3789
ApoI RAATTY 2 cut(s) 2281, 3024
AseI ATTAAT 1 cut(s) 4025
AsiGI ACCGGT 2 cut(s) 58, 725
Asp700I GAANNNNTTC 4 cut(s) 789, 1345, 2730, 3149
Asp718I GGTACC 1 cut(s) 1883
AspA2I CCTAGG 2 cut(s) 1958, 3706
AspLEI GCGC 4 cut(s) 221, 1066, 1582, 2820
AspS9I GGNCC 5 cut(s) 30, 223, 728, 2305, 4131
AsuII TTCGAA 2 cut(s) 1240, 1456
AsuNHI GCTAGC 1 cut(s) 815
AvaI CYCGRG 2 cut(s) 12, 83
AvaII GGWCC 5 cut(s) 30, 223, 728, 2305, 4131
AvrII CCTAGG 2 cut(s) 1958, 3706
BaeGI GKGCMC 1 cut(s) 198
BaeI ACNNNNGTAYC 4 cut(s) 1608, 1641, 3852, 3885
BalI TGGCCA 3 cut(s) 881, 2825, 3179
BanI GGYRCC 2 cut(s) 1883, 2449
BanII GRGCYC 3 cut(s) 2153, 3126, 3729
BauI CACGAG 1 cut(s) 1196
Bbv12I GWGCWC 2 cut(s) 3126, 3602
BceAI ACGGC 7 cut(s) 76, 82, 176, 297, 410, 500, 2171
BciT130I CCWGG 4 cut(s) 533, 1022, 1907, 3897
BciVI GTATCC 1 cut(s) 1604
BclI TGATCA 2 cut(s) 1129, 2640
BcoDI GTCTC 9 cut(s) 67, 463, 841, 1435, 2236, 2458, 2608, 3545, 4166
BcuI ACTAGT 1 cut(s) 1870
BfmI CTRYAG 4 cut(s) 1150, 2587, 3127, 3564
BfoI RGCGCY 1 cut(s) 1067
BfuAI ACCTGC 2 cut(s) 224, 1495
BfuI GTATCC 1 cut(s) 1604
BglI GCCNNNNNGGC 1 cut(s) 269
BglII AGATCT 2 cut(s) 1774, 3667
BlnI CCTAGG 2 cut(s) 1958, 3706
BlpI GCTNAGC 1 cut(s) 1639
BmcAI AGTACT 1 cut(s) 1689
Bme1390I CCNGG 4 cut(s) 533, 1022, 1907, 3897
Bme18I GGWCC 5 cut(s) 30, 223, 728, 2305, 4131
BmeT110I CYCGRG 2 cut(s) 12, 83
BmgT120I GGNCC 5 cut(s) 30, 223, 728, 2305, 4131
BmrFI CCNGG 4 cut(s) 533, 1022, 1907, 3897
BmsI GCATC 9 cut(s) 787, 1525, 1956, 2821, 2910, 3024, 3055, 3056, 4167
BmtI GCTAGC 1 cut(s) 819
BoxI GACNNNNGTC 2 cut(s) 59, 494
BpmI CTGGAG 3 cut(s) 768, 1466, 3879
Bpu1102I GCTNAGC 1 cut(s) 1639
Bpu14I TTCGAA 2 cut(s) 1240, 1456
BpuEI CTTGAG 4 cut(s) 187, 1622, 2632, 3494
BsaAI YACGTR 1 cut(s) 43
BsaI GGTCTC 3 cut(s) 67, 463, 4166
BsaJI CCNNGG 6 cut(s) 264, 1090, 1905, 1958, 2187, 3706
BsaWI WCCGGW 4 cut(s) 58, 725, 2053, 2100
Bsc4I CCNNNNNNNGG 8 cut(s) 204, 352, 1375, 1400, 1444, 1921, 3641, 4190
Bse118I RCCGGY 3 cut(s) 58, 225, 725
Bse1I ACTGG 8 cut(s) 1449, 1732, 1970, 2095, 2272, 2966, 3635, 3660
Bse3DI GCAATG 5 cut(s) 2643, 2760, 2985, 3232, 4077
BseAI TCCGGA 2 cut(s) 2053, 2100
BseBI CCWGG 4 cut(s) 533, 1022, 1907, 3897
BseDI CCNNGG 6 cut(s) 264, 1090, 1905, 1958, 2187, 3706
BseLI CCNNNNNNNGG 8 cut(s) 204, 352, 1375, 1400, 1444, 1921, 3641, 4190
BseMI GCAATG 5 cut(s) 2643, 2760, 2985, 3232, 4077
BseMII CTCAG 8 cut(s) 78, 931, 1263, 1430, 1487, 2478, 3453, 3518
BseNI ACTGG 8 cut(s) 1449, 1732, 1970, 2095, 2272, 2966, 3635, 3660
BseRI GAGGAG 4 cut(s) 161, 164, 182, 3227
BseSI GKGCMC 1 cut(s) 198
BsgI GTGCAG 1 cut(s) 3234
Bsh1236I CGCG 4 cut(s) 122, 221, 266, 707
Bsh1285I CGRYCG 1 cut(s) 261
BshFI GGCC 9 cut(s) 339, 881, 975, 1089, 1914, 2186, 2825, 3179, 3791
BshNI GGYRCC 2 cut(s) 1883, 2449
BshTI ACCGGT 2 cut(s) 58, 725
BsiEI CGRYCG 1 cut(s) 261
BsiHKAI GWGCWC 2 cut(s) 3126, 3602
BsiHKCI CYCGRG 2 cut(s) 12, 83
BsiSI CCGG 5 cut(s) 59, 226, 726, 2054, 2101
BslFI GGGAC 4 cut(s) 16, 292, 584, 4117
BslI CCNNNNNNNGG 8 cut(s) 204, 352, 1375, 1400, 1444, 1921, 3641, 4190
BsmAI GTCTC 9 cut(s) 67, 463, 841, 1435, 2236, 2458, 2608, 3545, 4166
BsmFI GGGAC 4 cut(s) 16, 292, 584, 4117
BsmI GAATGC 3 cut(s) 1840, 3095, 3946
BsnI GGCC 9 cut(s) 339, 881, 975, 1089, 1914, 2186, 2825, 3179, 3791
Bso31I GGTCTC 3 cut(s) 67, 463, 4166
BsoBI CYCGRG 2 cut(s) 12, 83
Bsp119I TTCGAA 2 cut(s) 1240, 1456
Bsp1286I GDGCHC 5 cut(s) 198, 2153, 3126, 3602, 3729
Bsp13I TCCGGA 2 cut(s) 2053, 2100
Bsp1407I TGTACA 1 cut(s) 1219
Bsp1720I GCTNAGC 1 cut(s) 1639
BspANI GGCC 9 cut(s) 339, 881, 975, 1089, 1914, 2186, 2825, 3179, 3791
BspCNI CTCAG 8 cut(s) 77, 930, 1264, 1431, 1488, 2479, 3452, 3519
BspEI TCCGGA 2 cut(s) 2053, 2100
BspFNI CGCG 4 cut(s) 122, 221, 266, 707
BspMI ACCTGC 2 cut(s) 224, 1495
BspOI GCTAGC 1 cut(s) 819
BspPI GGATC 8 cut(s) 341, 405, 1270, 1385, 1554, 2336, 3285, 3626
BspQI GCTCTTC 3 cut(s) 1624, 1647, 2913
BspT104I TTCGAA 2 cut(s) 1240, 1456
BspT107I GGYRCC 2 cut(s) 1883, 2449
BspTNI GGTCTC 3 cut(s) 67, 463, 4166
BsrBI CCGCTC 1 cut(s) 1381
BsrDI GCAATG 5 cut(s) 2643, 2760, 2985, 3232, 4077
BsrFI RCCGGY 3 cut(s) 58, 225, 725
BsrGI TGTACA 1 cut(s) 1219
BsrI ACTGG 8 cut(s) 1449, 1732, 1970, 2095, 2272, 2966, 3635, 3660
BssAI RCCGGY 3 cut(s) 58, 225, 725
BssECI CCNNGG 6 cut(s) 264, 1090, 1905, 1958, 2187, 3706
BssSI CACGAG 1 cut(s) 1196
BssT1I CCWWGG 3 cut(s) 1090, 1958, 3706
Bst2BI CACGAG 1 cut(s) 1196
Bst2UI CCWGG 4 cut(s) 533, 1022, 1907, 3897
Bst6I CTCTTC 6 cut(s) 1624, 1647, 2023, 2553, 2781, 2913
BstAPI GCANNNNNTGC 1 cut(s) 3101
BstAUI TGTACA 1 cut(s) 1219
BstBAI YACGTR 1 cut(s) 43
BstBI TTCGAA 2 cut(s) 1240, 1456
BstDSI CCRYGG 2 cut(s) 264, 2187
BstENI CCTNNNNNAGG 1 cut(s) 202
BstFNI CGCG 4 cut(s) 122, 221, 266, 707
BstH2I RGCGCY 1 cut(s) 1067
BstHHI GCGC 4 cut(s) 221, 1066, 1582, 2820
BstMAI GTCTC 9 cut(s) 67, 463, 841, 1435, 2236, 2458, 2608, 3545, 4166
BstMCI CGRYCG 1 cut(s) 261
BstNI CCWGG 4 cut(s) 533, 1022, 1907, 3897
BstNSI RCATGY 3 cut(s) 2098, 3869, 4085
BstPAI GACNNNNGTC 2 cut(s) 59, 494
BstSCI CCNGG 4 cut(s) 531, 1020, 1905, 3895
BstSFI CTRYAG 4 cut(s) 1150, 2587, 3127, 3564
BstSLI GKGCMC 1 cut(s) 198
BstUI CGCG 4 cut(s) 122, 221, 266, 707
BstX2I RGATCY 5 cut(s) 397, 1275, 1774, 3277, 3667
BstXI CCANNNNNNTGG 2 cut(s) 2460, 2821
BstYI RGATCY 5 cut(s) 397, 1275, 1774, 3277, 3667
BsuI GTATCC 1 cut(s) 1604
BsuRI GGCC 9 cut(s) 339, 881, 975, 1089, 1914, 2186, 2825, 3179, 3791
BtgI CCRYGG 2 cut(s) 264, 2187
BtgZI GCGATG 1 cut(s) 3458
BtsI GCAGTG 2 cut(s) 902, 3246
BtsIMutI CAGTG 8 cut(s) 673, 902, 1725, 1977, 2567, 2803, 3084, 3246
BveI ACCTGC 2 cut(s) 224, 1495
CaiI CAGNNNCTG 1 cut(s) 3641
CfoI GCGC 4 cut(s) 221, 1066, 1582, 2820
Cfr10I RCCGGY 3 cut(s) 58, 225, 725
Cfr13I GGNCC 5 cut(s) 30, 223, 728, 2305, 4131
Cfr42I CCGCGG 1 cut(s) 267
CpoI CGGWCCG 1 cut(s) 223
CseI GACGC 3 cut(s) 111, 128, 4193
CspAI ACCGGT 2 cut(s) 58, 725
CspCI CAANNNNNGTGG 2 cut(s) 3981, 4016
CspI CGGWCCG 1 cut(s) 223
DrdI GACNNNNNNGTC 1 cut(s) 253
DseDI GACNNNNNNGTC 1 cut(s) 253
EaeI YGGCCR 4 cut(s) 337, 879, 2823, 3177
Eam1104I CTCTTC 6 cut(s) 1624, 1647, 2023, 2553, 2781, 2913
EarI CTCTTC 6 cut(s) 1624, 1647, 2023, 2553, 2781, 2913
Ecl136II GAGCTC 1 cut(s) 3124
Eco130I CCWWGG 3 cut(s) 1090, 1958, 3706
Eco24I GRGCYC 3 cut(s) 2153, 3126, 3729
Eco31I GGTCTC 3 cut(s) 67, 463, 4166
Eco32I GATATC 2 cut(s) 9, 2337
Eco47I GGWCC 5 cut(s) 30, 223, 728, 2305, 4131
Eco47III AGCGCT 1 cut(s) 1065
Eco53kI GAGCTC 1 cut(s) 3124
Eco57I CTGAAG 3 cut(s) 828, 2063, 3161
Eco88I CYCGRG 2 cut(s) 12, 83
EcoICRI GAGCTC 1 cut(s) 3124
EcoNI CCTNNNNNAGG 1 cut(s) 202
EcoO109I RGGNCCY 1 cut(s) 4131
EcoRI GAATTC 1 cut(s) 3024
EcoRII CCWGG 4 cut(s) 531, 1020, 1905, 3895
EcoRV GATATC 2 cut(s) 9, 2337
EcoT14I CCWWGG 3 cut(s) 1090, 1958, 3706
EcoT22I ATGCAT 2 cut(s) 802, 862
EcoT38I GRGCYC 3 cut(s) 2153, 3126, 3729
ErhI CCWWGG 3 cut(s) 1090, 1958, 3706
FalI AAGNNNNNCTT 2 cut(s) 510, 542
FaqI GGGAC 4 cut(s) 16, 292, 584, 4117
FauI CCCGC 3 cut(s) 42, 1524, 2764
FauNDI CATATG 3 cut(s) 802, 1525, 3273
FbaI TGATCA 2 cut(s) 1129, 2640
FblI GTMKAC 1 cut(s) 2625
FriOI GRGCYC 3 cut(s) 2153, 3126, 3729
FspAI RTGCGCAY 1 cut(s) 1581
FspI TGCGCA 1 cut(s) 1581
GlaI GCGC 4 cut(s) 220, 1065, 1581, 2819
GsuI CTGGAG 3 cut(s) 768, 1466, 3879
HaeII RGCGCY 1 cut(s) 1067
HaeIII GGCC 9 cut(s) 339, 881, 975, 1089, 1914, 2186, 2825, 3179, 3791
HapII CCGG 5 cut(s) 59, 226, 726, 2054, 2101
HgaI GACGC 3 cut(s) 111, 128, 4193
HhaI GCGC 4 cut(s) 221, 1066, 1582, 2820
Hin6I GCGC 4 cut(s) 219, 1064, 1580, 2818
HinP1I GCGC 4 cut(s) 219, 1064, 1580, 2818
HincII GTYRAC 4 cut(s) 1868, 3490, 3886, 4087
HindII GTYRAC 4 cut(s) 1868, 3490, 3886, 4087
HpaII CCGG 5 cut(s) 59, 226, 726, 2054, 2101
Hpy166II GTNNAC 9 cut(s) 213, 295, 1733, 1868, 2626, 3246, 3490, 3886, 4087
Hpy8I GTNNAC 9 cut(s) 213, 295, 1733, 1868, 2626, 3246, 3490, 3886, 4087
Hpy99I CGWCG 4 cut(s) 97, 122, 127, 377
HpyCH4IV ACGT 4 cut(s) 42, 851, 875, 2291
HpySE526I ACGT 4 cut(s) 42, 851, 875, 2291
HspAI GCGC 4 cut(s) 219, 1064, 1580, 2818
KflI GGGWCCC 1 cut(s) 4131
Kpn2I TCCGGA 2 cut(s) 2053, 2100
KpnI GGTACC 1 cut(s) 1887
Ksp22I TGATCA 2 cut(s) 1129, 2640
KspI CCGCGG 1 cut(s) 267
LguI GCTCTTC 3 cut(s) 1624, 1647, 2913
LweI GCATC 9 cut(s) 787, 1525, 1956, 2821, 2910, 3024, 3055, 3056, 4167
MaeII ACGT 4 cut(s) 42, 851, 875, 2291
MaeIII GTNAC 9 cut(s) 1474, 1723, 2133, 2253, 2322, 2575, 2606, 2662, 3107
MbiI CCGCTC 1 cut(s) 1381
MfeI CAATTG 1 cut(s) 1674
MflI RGATCY 5 cut(s) 397, 1275, 1774, 3277, 3667
MhlI GDGCHC 5 cut(s) 198, 2153, 3126, 3602, 3729
MlsI TGGCCA 3 cut(s) 881, 2825, 3179
MluI ACGCGT 1 cut(s) 120
MluNI TGGCCA 3 cut(s) 881, 2825, 3179
MlyI GAGTC 5 cut(s) 95, 2009, 2141, 3886, 4209
MmeI TCCRAC 8 cut(s) 54, 428, 613, 991, 1418, 1606, 2151, 3527
Mox20I TGGCCA 3 cut(s) 881, 2825, 3179
Mph1103I ATGCAT 2 cut(s) 802, 862
MroI TCCGGA 2 cut(s) 2053, 2100
MroXI GAANNNNTTC 4 cut(s) 789, 1345, 2730, 3149
MscI TGGCCA 3 cut(s) 881, 2825, 3179
MseI TTAA 9 cut(s) 528, 1026, 1082, 1191, 1502, 1901, 2121, 4025, 4145
MslI CAYNNNNRTG 4 cut(s) 1047, 1850, 2397, 3855
Msp20I TGGCCA 3 cut(s) 881, 2825, 3179
MspA1I CMGCKG 4 cut(s) 188, 266, 443, 3139
MspI CCGG 5 cut(s) 59, 226, 726, 2054, 2101
MspR9I CCNGG 4 cut(s) 533, 1022, 1907, 3897
MunI CAATTG 1 cut(s) 1674
Mva1269I GAATGC 3 cut(s) 1840, 3095, 3946
MvaI CCWGG 4 cut(s) 533, 1022, 1907, 3897
MvnI CGCG 4 cut(s) 122, 221, 266, 707
NdeI CATATG 3 cut(s) 802, 1525, 3273
NheI GCTAGC 1 cut(s) 815
NmeAIII GCCGAG 2 cut(s) 1792, 3817
NmuCI GTSAC 6 cut(s) 1474, 1723, 2133, 2322, 2606, 2662
NsbI TGCGCA 1 cut(s) 1581
NsiI ATGCAT 2 cut(s) 802, 862
NspI RCATGY 3 cut(s) 2098, 3869, 4085
NspV TTCGAA 2 cut(s) 1240, 1456
PaqCI CACCTGC 1 cut(s) 224
PciSI GCTCTTC 3 cut(s) 1624, 1647, 2913
PcsI WCGNNNNNNNCGW 3 cut(s) 95, 131, 872
PctI GAATGC 3 cut(s) 1840, 3095, 3946
PdmI GAANNNNTTC 4 cut(s) 789, 1345, 2730, 3149
PfeI GAWTC 9 cut(s) 469, 684, 963, 1099, 2192, 2312, 3983, 3989, 4116
PflFI GACNNNGTC 1 cut(s) 954
PflMI CCANNNNNTGG 1 cut(s) 3641
PfoI TCCNGGA 1 cut(s) 3895
PinAI ACCGGT 2 cut(s) 58, 725
Ple19I CGATCG 1 cut(s) 261
PleI GAGTC 5 cut(s) 94, 2009, 2140, 3886, 4209
PpsI GAGTC 5 cut(s) 94, 2009, 2140, 3886, 4209
Ppu21I YACGTR 1 cut(s) 43
PpuMI RGGWCCY 1 cut(s) 4131
PshAI GACNNNNGTC 2 cut(s) 59, 494
PshBI ATTAAT 1 cut(s) 4025
PsiI TTATAA 4 cut(s) 1058, 1829, 2442, 3681
Psp124BI GAGCTC 1 cut(s) 3126
Psp1406I AACGTT 1 cut(s) 875
Psp5II RGGWCCY 1 cut(s) 4131
Psp6I CCWGG 4 cut(s) 531, 1020, 1905, 3895
PspGI CCWGG 4 cut(s) 531, 1020, 1905, 3895
PspPI GGNCC 5 cut(s) 30, 223, 728, 2305, 4131
PspPPI RGGWCCY 1 cut(s) 4131
PsrI GAACNNNNNNTAC 2 cut(s) 3507, 3539
PstNI CAGNNNCTG 1 cut(s) 3641
PsuI RGATCY 5 cut(s) 397, 1275, 1774, 3277, 3667
PsyI GACNNNGTC 1 cut(s) 954
PvuI CGATCG 1 cut(s) 261
PvuII CAGCTG 3 cut(s) 188, 443, 3139
RseI CAYNNNNRTG 4 cut(s) 1047, 1850, 2397, 3855
Rsr2I CGGWCCG 1 cut(s) 223
RsrII CGGWCCG 1 cut(s) 223
SacI GAGCTC 1 cut(s) 3126
SacII CCGCGG 1 cut(s) 267
SapI GCTCTTC 3 cut(s) 1624, 1647, 2913
SaqAI TTAA 9 cut(s) 528, 1026, 1082, 1191, 1502, 1901, 2121, 4025, 4145
Sau96I GGNCC 5 cut(s) 30, 223, 728, 2305, 4131
ScaI AGTACT 1 cut(s) 1689
SchI GAGTC 5 cut(s) 95, 2009, 2141, 3886, 4209
ScrFI CCNGG 4 cut(s) 533, 1022, 1907, 3897
SduI GDGCHC 5 cut(s) 198, 2153, 3126, 3602, 3729
SfaNI GCATC 9 cut(s) 787, 1525, 1956, 2821, 2910, 3024, 3055, 3056, 4167
SfcI CTRYAG 4 cut(s) 1150, 2587, 3127, 3564
Sfr303I CCGCGG 1 cut(s) 267
SfuI TTCGAA 2 cut(s) 1240, 1456
SgrBI CCGCGG 1 cut(s) 267
SinI GGWCC 5 cut(s) 30, 223, 728, 2305, 4131
SmiMI CAYNNNNRTG 4 cut(s) 1047, 1850, 2397, 3855
SmlI CTYRAG 4 cut(s) 202, 1601, 2611, 3473
SmoI CTYRAG 4 cut(s) 202, 1601, 2611, 3473
SpeI ACTAGT 1 cut(s) 1870
SspI AATATT 7 cut(s) 790, 1080, 1231, 1519, 2874, 3150, 3760
SstI GAGCTC 1 cut(s) 3126
StyD4I CCNGG 4 cut(s) 531, 1020, 1905, 3895
StyI CCWWGG 3 cut(s) 1090, 1958, 3706
TaiI ACGT 4 cut(s) 45, 854, 878, 2294
TatI WGTACW 5 cut(s) 294, 325, 1219, 1687, 2432
TfiI GAWTC 9 cut(s) 469, 684, 963, 1099, 2192, 2312, 3983, 3989, 4116
Tru1I TTAA 9 cut(s) 528, 1026, 1082, 1191, 1502, 1901, 2121, 4025, 4145
Tru9I TTAA 9 cut(s) 528, 1026, 1082, 1191, 1502, 1901, 2121, 4025, 4145
TscAI CASTG 8 cut(s) 673, 909, 1732, 1977, 2574, 2803, 3091, 3253
TseFI GTSAC 6 cut(s) 1474, 1723, 2133, 2322, 2606, 2662
Tsp45I GTSAC 6 cut(s) 1474, 1723, 2133, 2322, 2606, 2662
TspGWI ACGGA 3 cut(s) 1664, 4122, 4160
TspRI CASTG 8 cut(s) 673, 909, 1732, 1977, 2574, 2803, 3091, 3253
Tth111I GACNNNGTC 1 cut(s) 954
Van91I CCANNNNNTGG 1 cut(s) 3641
VpaK11BI GGWCC 5 cut(s) 30, 223, 728, 2305, 4131
VspI ATTAAT 1 cut(s) 4025
XagI CCTNNNNNAGG 1 cut(s) 202
XapI RAATTY 2 cut(s) 2281, 3024
XbaI TCTAGA 2 cut(s) 1771, 3058
XceI RCATGY 3 cut(s) 2098, 3869, 4085
XcmI CCANNNNNNNNNTGG 2 cut(s) 2294, 3955
XmaJI CCTAGG 2 cut(s) 1958, 3706
XmiI GTMKAC 1 cut(s) 2625
XmnI GAANNNNTTC 4 cut(s) 789, 1345, 2730, 3149
ZrmI AGTACT 1 cut(s) 1689
Zsp2I ATGCAT 2 cut(s) 802, 862
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.